WO2010060085A2 - Appareil et procédé pour le traitement des microorganismes dans des procédés de production de sucre et de fermentation à base de sucre - Google Patents

Appareil et procédé pour le traitement des microorganismes dans des procédés de production de sucre et de fermentation à base de sucre Download PDF

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WO2010060085A2
WO2010060085A2 PCT/US2009/065737 US2009065737W WO2010060085A2 WO 2010060085 A2 WO2010060085 A2 WO 2010060085A2 US 2009065737 W US2009065737 W US 2009065737W WO 2010060085 A2 WO2010060085 A2 WO 2010060085A2
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clo
solution
sugar
vessel
stream
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PCT/US2009/065737
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WO2010060085A3 (fr
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Allen M. Ziegler
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Resonant Biosciences, Llc
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Publication of WO2010060085A3 publication Critical patent/WO2010060085A3/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/005—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor after treatment of microbial biomass not covered by C12N1/02 - C12N1/08
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/12—Bioreactors or fermenters specially adapted for specific uses for producing fuels or solvents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M43/00—Combinations of bioreactors or fermenters with other apparatus
    • C12M43/02—Bioreactors or fermenters combined with devices for liquid fuel extraction; Biorefineries
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C12N1/18—Baker's yeast; Brewer's yeast
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
    • C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Definitions

  • the technical field involves sugar production and sugar-based fermentation processes. Specifically, it is a method of reducing the concentration of undesirable microorganisms during sugar production and/or sugar-based fermentation processes while simultaneously encouraging propagation and/or conditioning of desirable microorganisms and increasing the efficiency of desirable microorganisms during sugar-based fermentation processes.
  • Sugars are a class of water-soluble crystalline carbohydrates. Examples of sugars include sucrose, fructose, glucose and lactose. Sugars have a characteristically sweet taste and are commonly used as sweeteners in many foods, drinks and medicines.
  • Sugars can also be used in fermentation processes.
  • microorganisms such as yeast, fungi and bacteria convert the sugars into cellular energy and produce aliphatic alcohols as byproducts.
  • These fermentation processes can be used to produce items such as industrial grade ethanol, distilled spirits, beer, wine, pharmaceuticals and nutraceuticals (foodstuff that provides health benefits, such as fortified foods and dietary supplements).
  • Sugars for consumption and/or fermentation processes can be derived from a number of sources. Sugars primarily come from sugar cane and from sugar beets, but also appear in fruit, honey, sorghum, sugar maple and in many other sources.
  • the starting material goes through a treatment process which produces an extraction which can then be treated for sale as consumable sugar or sent into a fermentation process. It is typical for a single facility to treat the starting materials and then alternate between sugar production and production of a fermentation product, such as ethanol.
  • the stream of material being treated can become contaminated with bacteria or other undesirable microorganisms. This can occur in one of the many vessels used in the starting material preparation process, the sugar production process and/or the overall fermentation process
  • bacteria or other undesirable microorganisms can grow much more rapidly than the desirable producing microorganisms.
  • the bacteria or other microorganisms compete with the desirable producing microorganisms for fermentable sugars and retard the desired bio-chemical reaction resulting in a lower product yield.
  • Bacteria also produce unwanted chemical by-products, which can cause spoilage of entire fermentation batches. Removing these bacteria or other undesirable microorganisms allows the desirable producing microorganisms to thrive, which results in higher efficiency.
  • antibiotics are expensive and can add greatly to the costs of large-scale production. Improved technology that refines and improves the efficiency of existing techniques would be of considerable value to the industry. Moreover, antibiotics are not effective against all strains of bacteria, such as antibiotic-resistant strains of bacteria. Overuse of antibiotics can lead to the creation of additional variants of antibiotic-resistant strains of bacteria. Antibiotic residues and establishment of antibiotic-resistant strains is a global issue. These concerns may lead to future regulatory action against the use of antibiotics.
  • Another approach involves washing the desirable producing microorganisms with phosphoric acid. This method does not effectively kill bacteria and other microorganisms. It can also stress the desirable producing microorganisms, thereby lowering their efficiency.
  • Chlorine dioxide (ClO 2 ) has many industrial and municipal uses. When produced and handled properly, ClO 2 is an effective and powerful biocide, disinfectant and oxidizer. ClO 2 has been used as a disinfectant in the food and beverage industries, wastewater treatment, industrial water treatment, cleaning and disinfections of medical wastes, textile bleaching, odor control for the rendering industry, circuit board cleansing in the electronics industry, and uses in the oil and gas industry. It is an effective biocide at low concentrations and over a wide pH range. ClO 2 is desirable because when it reacts with an organism in water, it reduces to chlorite ion and then to chloride, which studies to date have shown does not pose a significant adverse risk to human health. ClO 2 is, however, unstable in the gas phase and will readily undergo decomposition into chlorine gas (Cl 2 ), oxygen gas (O 2 ), and heat.
  • the current disclosure relates to a method for reducing the concentration of bacteria and other undesirable microorganisms during sugar production and sugar-based fermentation processes while simultaneously encouraging propagation and/or conditioning of desirable microorganisms and increasing the efficiency of those desirable microorganisms in the sugar-based fermentation processes and an apparatus for carrying out this method.
  • Certain embodiments of the current method comprise the steps of:
  • Certain embodiments of the current method comprise the steps of: (a) employing an aqueous fluid stream in a sugar production process; and
  • Certain embodiments of the current method comprise the steps of:
  • Certain embodiments of the current method comprise the steps of:
  • Certain embodiments of the present apparatus comprise:
  • a batch tank fluidly connected to the ClO 2 generator outlet, the batch tank receiving the ClO 2 gas stream from the ClO 2 generator outlet, the batch tank comprising an inlet for introducing a second water stream and an outlet for exhausting an aqueous ClO 2 solution from the batch tank;
  • FTG. 1 is a flow diagram of the process for production of sugar and sugar-based fermentation products.
  • FTG. 2 is a schematic of combined sugar and sugar-based fermentation equipment with an integrated ClO 2 system in accordance with one embodiment.
  • the current disclosure relates to a method for reducing the concentration of bacteria and other undesirable microorganisms during sugar production and/or sugar-based fermentation processes while simultaneously encouraging propagation and/or conditioning of desirable microorganisms and increasing the efficiency of those desirable microorganisms in the sugar-based fermentation processes and an apparatus for carrying out this method.
  • FTG. 1 illustrates the process for production of sugar and/or a sugar-based fermentation product. Production of sugar and production of a sugar-based fermentation product begin in a similar manner. At a certain point the processes diverge to obtain different end products. It is typical for a single facility to alternate between sugar production and production of a sugar-based fermentation product. For this reason the current disclosure examines the two processes together.
  • Examples of possible starting materials include sugar cane, sugar beets, fruit (such as citrus pulp), honey, sorghum, and sugar maple.
  • the starting material undergoes processing to extract the sugar. This processing can involve washing the starting material and cutting it into small pieces. These pieces can then be mixed with water and repeatedly crushed between rollers. This crushing or milling extracts a liquid containing about 15-20 percent by weight sucrose from the starting material. This liquid is sometimes called thin juice or sugar juice. The thin juice begins to ferment almost immediately. A solid also remains. The solid can be used for animal feed, in paper manufacture, or burned as fuel.
  • non-oxidizing biocides include Glutaraldehyde, 1,5 pentanedial Use Rat (1-100 ppm), 2,2-dibromo-3-nitrilopropionamide (1 -30 ppm), 5- chloro-N-methylisothiazolone & N-methylisothiazolone ( Typically referred to as Isothiazolone) (1-30 ppm), 1,2-benzisothiazolone (1-30 ppm), Thiocarbamates, Potassium N-dimethyldithiocarbamate (1-30 ppm), Poly-Quats, Poly[oxyethylene(dimethylinimio)ethylene- (dimethylinimio)ethylene dichloride] (1-30 ppm). Oxidizing biocides were also used. Examples of oxidizing biocides include bromine, sodium hypochlorite and calcium hypochlorite
  • the thin juice can then undergo further treatment.
  • the pH of the thin juice can be adjusted. This is sometimes done using lime. This adjustment arrests sucrose's decay into glucose and fructose, and precipitates out some impurities.
  • the mixture can then be allowed to sit, allowing suspended solids to settle out. This results in a clarified thin juice.
  • the clarified thin juice can alternatively enter into a sugar-based fermentation process.
  • the by-product liquid remaining after sugar crystalization could also enter into a sugar-based fermentation process.
  • the sugar source used in a fermentation process is typically referred to as molasses.
  • molasses There are several different types of molasses.
  • high test molasses is the name for the thin juice removed from milling or crushing sugar cane.
  • Blackstrap molasses is the by-product liquid produced during the milling and crushing of sugar cane for sugar production.
  • Refiners cane is the by-product liquid produced during the milling and crushing of brown sugar to produce white sugar.
  • Beet molasses is the by-product liquid produced during the milling and crushing of sugar beets for sugar production.
  • Citrus molasses is the name for sugar juices extracted from citrus pulp. Unlike carbohydrate- based fermentation processes which contain starch, all sugars in the molasses are present and readily available in a fermentable form. Molasses generally do not require cooking and are present in liquid form.
  • the yeast can undergo conditioning.
  • Conditioning is the second step in the overall fermentation process.
  • the objectives of both propagation and conditioning are to deliver a large volume of yeast to the fermentation tank with high viability, high budding and a low level of infection by other microorganisms.
  • conditioning is unlike propagation in that it does not involve growing a large quantity from a small lab culture.
  • conditions are provided to re-hydrate the yeast, bring them out of hibernation and allow for maximum anaerobic growth and reproduction.
  • the yeast Following propagation and/or conditioning, the yeast enter the fermentation step of the overall fermentation process.
  • the yeast produce energy by converting the sugars into carbon dioxide and aliphatic alcohols, such as ethanol.
  • the fermented molasses now called “beer” now enters the processing steps of the overall fermentation process.
  • the beer is distilled. This process removes the 190 proof ethanol, a type of alcohol, from the solids in the fermented molasses. After distillation, the alcohol is passed through a dehydration system to remove remaining water. At this point the product is 200 proof ethanol. This ethanol can then be denatured by adding a small amount of denaturant, such as gasoline, to make it unfit for human consumption.
  • denaturant such as gasoline
  • the overall fermentation process can be carried out using batch and continuous methods.
  • the batch process is used for small- scale production. Each batch is completed before a new one begins.
  • the continuous fermentation method is used for large-scale production because it produces a continuous supply without restarting every time.
  • the current method and apparatus are effective for both methods.
  • Sugar-based ethanol facilities typically recycle yeast. These facilities use a yeast centrifuge and yeast process tanks to remove yeast from completed fermentations for reuse. After two to four months, new yeast can be added to the system to recharge the system with fresh yeast. The current method and apparatus are effective for a facility that recycles yeast.
  • the "undesirable" microorganisms intended to be reduced are those that compete for nutrients with the desirable microorganisms, such as yeast that produce fermentation products in the fermentation processes involved here.
  • the aqueous ClO 2 solution employed in the present method does not appear to detrimentally affect the growth and viability of desirable, fermentation-promoting microorganisms, but does appear to eliminate or at least suppress the growth of undesirable microorganisms that interfere with the fermentation process.
  • the elimination or suppression of undesirable microorganisms appears to have a favorable effect on the growth and viability of desirable microorganisms, for the reasons set forth in the Background section.
  • Yeast can withstand and indeed thrive in a ClO 2 environment. However, bacteria, wild yeasts, killer yeasts and molds will succumb to the properties of ClO 2 allowing the producing, desirable yeast to thrive and achieve higher production
  • ClO 2 solution has many uses in disinfection, bleaching and chemical oxidation.
  • ClO 2 can be added at various points in the starting material preparation process, the raw sugar production process and/or the overall fermentation process to kill unwanted microorganisms and promote growth and survival of the desirable microorganisms.
  • This ClO 2 can be added as an aqueous solution or a gas.
  • the ClO 2 can be added during the starting material preparation process, the raw sugar production process and/or the overall fermentation process.
  • the ClO 2 solution can be added to milling vessels, thick juice treatment vessels, thin juice treatment vessels, vacuum pans, sugar crystallizers, evaporators, transfer lines, yeast recycle tanks, yeast separators, centrifuges, beer wells, cook vessels, fermentation tanks, propagation tanks, conditioning tanks, starter tanks or tanks used during liquefaction.
  • the ClO 2 solution can also be added to the interstage heat exchange system or heat exchangers.
  • the ClO 2 has an efficiency as ClO 2 in the stream of at least about 90%. Adding ClO 2 having a known purity allows for addition of a controlled amount of ClO 2 .
  • Chorine dioxide is a selective oxidizer. It provides microbial efficacy in high organic processes that exceeds that of other antimicrobials.
  • the selectivity of the chlorine dioxide allows for removal of the bio-film discussed above due to its affinity to oxidize disulfide bonds before reacting with other constituents. When the disulfide bonds that make up the backbone of the bio-film are broken, the film can no longer remain connected to the pipe. Initially when the bio-film is being destroyed more bacteria will be exposed to the process since they tend to inhabit the area under the film. Once the bio-film is removed a cleaner system can be realized.
  • ClO 2 can be added during the milling/crush of the starting material.
  • Chlorine dioxide can be added in an effective amount.
  • chlorine dioxide dosages of about 20 to about 80 mg/L can be applied during the milling/crush of the starting material.
  • ClO 2 can be added to the thin juice.
  • Chlorine dioxide can be added in an effective amount.
  • chlorine dioxide dosages of about 10 to about 50, mg/L, or about 20 to about 80 mg/L can be applied to the thin juice.
  • Application of chlorine dioxide to the thin juice line keeps bio-film from forming in the pipeline and reduces the initial count of bacteria going into the distillery.
  • the ClO 2 can also be added during the sugar juice treatment steps to either the thin juice or the thick juice.
  • Chlorine dioxide can be added in an effective amount. As one example, chlorine dioxide dosages of about 10 to about 50 mg/L can be applied directly to the thin juice or thick juice.
  • the ClO 2 can also be added to the evaporators, vacuum pans or crystallizers used during the raw sugar production process. Chlorine dioxide can be added in an effective amount. As one example, chlorine dioxide dosages of about 2 to about 50 mg/L can be applied to the evaporators, vacuum pans or crystallizers. [0059] The ClO 2 can also be added directly into the fermentation mixture. Chlorine dioxide can be added in an effective amount. As one example, chlorine dioxide dosages of about 2 to about 30 mg/L can be applied directly to the fermentation mixture.
  • Chlorine dioxide can also be added to the desirable microorganism recycle tank. Chlorine dioxide can be added in an effective amount. As one example, chlorine dioxide dosages of about 10 to about 85 mg/L can be added to the desirable microorganism recycle tank.
  • Chlorine dioxide can also be added to the yeast separator and/or centrifuge. Chlorine dioxide can be added in an effective amount. As one example, chlorine dioxide dosages of about 10 to about 85 mg/L can be added to the yeast separator and/or centrifuge.
  • Chlorine dioxide can also be added to the beer well. Chlorine dioxide can be added in an effective amount. As one example, chlorine dioxide dosages of about 2 to about 40 mg/L can be added to the beer well.
  • the ClO 2 can also be added to the transfer lines connecting the many vessels used in the starting material preparation process, the raw sugar production process and/or the overall fermentation process.
  • Chlorine dioxide can be added in an effective amount. As one example, chlorine dioxide dosages of about 1 to about 20 mg/L can be applied to the transfer lines.
  • Chlorine dioxide can also be added prior to the heat exchangers at the distillery on the thin juice line to prevent bio-film formation and reduce bacteria that may be remaining in the thin juice feed. Chlorine dioxide can also be injected at the heat exchanger on each fermenter to keep bacterial counts low as the fermenters allow for a bacterial breeding area.
  • a side product of sugar production and/or sugar-based fermentation is vinasse. It can be used as a feed supplement . Chlorine dioxide can also be injected into the vinasse to keep bacterial counts low as this stream is another area where bacteria have a chance to increase and further infect the process.
  • ClO 2 reduces to form chlorite ion and then further reduces to form chloride ion and/or salt.
  • the reduction from ClO 2 to chloride ion happens quickly and is indeterminate compared to the background residual already present.
  • the chloride ion is a non-hazardous byproduct unlike those created by many antibiotics. Studies to date have shown that chloride ion does not pose a significant adverse risk to human health.
  • ClO 2 gas can decompose explosively, it is typically produced on-site. There are a number of methods of producing ClO 2 gas having a known purity, which are known to persons familiar with the technology involved here. One or more of these methods can be used. ClO 2 gas can be produced using electrochemical cells and a sodium chlorite or sodium chlorate solution. An equipment based sodium chlorate/hydrogen peroxide method also exists. Alternatively, non-equipment based binary, multiple precursor dry or liquid precursor technologies can be used. Examples of non-equipment based methods of ClO 2 generation include dry mix chlorine dioxide packets that include both a chlorite precursor packet and an acid activator packet.
  • hypochlorous acid reacts with water to form hypochlorous acid and hydrochloric acid. These acids then react with sodium chlorite to form chlorine dioxide, water and sodium chloride.
  • sodium hypochlorite is combined with hydrochloric or other acid to form hypochlorous acid. Sodium chlorite is then added to this reaction mixture to produce chlorine dioxide.
  • the third method combines sodium chlorite and sufficient hydrochloric acid.
  • the ClO 2 gas produced is between 0.0005 and 5.0 % by weight in air.
  • the ClO 2 gas is dissolved in a solvent in order to create a ClO 2 solution.
  • ClO 2 gas is readily soluble in water.
  • the water and ClO 2 gas are combined in quantities that create an effective solution for application during the milling/crush of the starting material, as one example a concentration of about 20 to about 80 mg/L.
  • the water and ClO 2 gas are combined in quantities that create an effective solution for application to the thin juice, for example concentrations of about 20 to about 80 mg/L or about 10 to about 50 mg/L.
  • the water and ClO 2 gas are combined in quantities that create an effective solution for application during the sugar juice treatment steps to either the thin juice or the thick juice, as one example a concentration of about 10 to about 50 mg/L.
  • the water and ClO 2 gas are combined in quantities that create an effective solution for application to the evaporators, vacuum pans or crystallizers used during the raw sugar production process, as one example a concentration of about 2 to about 50 mg/L. In another embodiment the water and ClO 2 gas are combined in quantities that create an effective solution for application directly into the fermentation mixture, as one example a concentration of about 2 to about 30 mg/L. In another embodiment the water and ClO 2 gas are combined in quantities that create an effective solution for application during propagation and/or conditioning, as one example a concentration of about 10 to about 85 mg/L.
  • the water and ClO 2 gas are combined in quantities that create an effective solution for application to the desirable microorganism recycle tank, as one example a concentration of about 10 to about 85 mg/L. In another embodiment the water and ClO 2 gas are combined in quantities that create an effective solution for application to the yeast separator and/or centrifuge, as one example a concentration of about 10 to about 85 mg/L. In another embodiment the water and ClO 2 gas are combined in quantities that create an effective solution for application to the beer well, as one example a concentration of about 2 to about 40 mg/L. In another embodiment the water and ClO 2 gas are combined in quantities that create an effective solution for application to the transfer lines, as one example a concentration of about 1 to about 20 mg/L. In the solution of one embodiment the ClO 2 solution has an efficiency as ClO 2 in the stream of at least about 90%.
  • Pure or substantially pure ClO 2 is desirable because it allows the user to precisely maintain the amount of ClO 2 added to the yeast. (The single term “pure” will be used hereinafter to mean either pure or substantially pure.) If too little ClO 2 is added the dosage will not be effective in killing undesirable microorganisms. If too much ClO 2 is added it can kill the desirable yeast. If either of these situations occurs, the addition of ClO 2 will not result in more efficient ethanol production. Addition of pure ClO 2 allows the user to carefully monitor and adjust the amount of ClO 2 added to the yeast. This enables the user to add adequate ClO 2 to improve microbial efficacy without killing the yeast.
  • the ClO 2 solution is introduced at some point during the production of ethanol or sugar.
  • the ClO 2 solution can be added in the starting material preparation process, the raw sugar production process and/or the overall fermentation process.
  • the ClO 2 solution can be added to milling vessels, thick juice treatment vessels, thin juice treatment vessels, vacuum pans, sugar crystallizers, evaporators, transfer lines, yeast recycle tanks, yeast separators, centrifuges, beer wells, cook vessels, fermentation tanks, propagation tanks, conditioning tanks, starter tanks or tanks used during liquefaction.
  • the ClO 2 solution can also be added to the piping between these units or heat exchangers.
  • FTG. 2 illustrates an apparatus for carrying out the fermentation process with an integrated ClO 2 system.
  • the apparatus has a ClO 2 generator.
  • the ClO 2 generator has an input for electricity. There is also an inlet for at least one chlorine containing chemical.
  • the ClO 2 generator should also have an outlet for exhausting a ClO 2 gas stream from the generator. In one embodiment the ClO 2 gas stream exiting the generator is between 0.0005 and 5.0 % by weight in air.
  • a batch tank that receives the ClO 2 gas stream is fluidly connected to the ClO 2 generator outlet.
  • the ClO 2 gas is dissolved in water to form a ClO 2 solution.
  • the batch tank has an inlet for introducing a water stream.
  • the water stream and the ClO 2 gas stream are combined to form a ClO 2 solution.
  • the concentration of the ClO 2 solution in the batch tank can vary across a wide range. Concentrations of up to about 5,000 mg/L can be achieved and concentrations of up to about 8,000 mg/L can be achieved with additional equipment.
  • the ClO 2 solution is then exhausted from the batch tank through an outlet at a specified dosage rate to create a solution of the desired concentration.
  • the dosed ClO 2 solution, for application during the milling/crush of the starting material has an effective concentration, as one example about 20 to about 80 mg/L.
  • the dosed ClO 2 solution, for application to the thin juice has an effective concentration, for example about 20 to about 80 mg/L or about 10 to about 50 mg/L.
  • the dosed ClO 2 solution, for application during the sugar juice treatment steps to either the thin juice or the thick juice has an effective concentration, as one example about 10 to about 50 mg/L.
  • the dosed ClO 2 solution, for application to the evaporators, vacuum pans or crystallizers used during the raw sugar production process has an effective concentration, as one example about 2 to about 50 mg/L.
  • the dosed ClO 2 solution, for application directly into the fermentation mixture has an effective concentration, as one example about 2 to about 30 mg/L.
  • the dosed ClO 2 solution, for application during propagation and/or conditioning has an effective concentration, as one example about 10 to about 85 mg/L.
  • the dosed ClO 2 solution, for application to the desirable microorganism recycle tank has an effective concentration, as one example about 10 to about 85 mg/L.
  • the dosed ClO 2 solution, for application to the yeast separator and/or centrifuge has an effective concentration, as one example about 10 to about 85 mg/L.
  • the dosed ClO 2 solution, for application to the beer well has an effective concentration, as one example about 2 to about 40 mg/L.
  • the dosed ClO 2 solution, for application to the transfer lines has an effective concentration, as one example about 1 to about 20 mg/L.
  • the exiting ClO 2 solution has an efficiency as ClO 2 in the stream of at least about 90%.
  • a production vessel is fluidly connected to the batch tank via the ClO 2 solution outlet.
  • the production vessel could be a milling vessel, thick juice treatment vessel, thin juice treatment vessel, vacuum pan, sugar crystallizer, evaporator, transfer line, yeast recycle tank, yeast separator, centrifuge, beer well, cook vessel, fermentation tank, propagation tank, conditioning tank, starter tank or tank used during liquefaction.
  • Multiple production vessels could be fluidly connected to a single batch tank, as shown in FTG. 2.
  • Introducing the ClO 2 solution into the production vessel is capable of decreasing the concentration of undesirable microorganisms and potentially also promoting propagation of yeast present.
  • a thin juice line at a sugar plant that fed into a distillery for fermentation was treated with chlorine dioxide according to the present method.
  • the thin juice line had been treated using sulfuric acid to decrease the pH.
  • the trial evaluated the bacterial efficacy of chlorine dioxide at an elevated pH in order to reduce sulfuric acid use without causing a detrimental effect to the yeast or fermentation.
  • the data indicates a low level of lactate before chlorine dioxide was introduced at the normal pH of 3.5.
  • the lactate trended downward once the chlorine dioxide treatment was started at 20 ppm with a pH elevation to 4.
  • the equipment had to be shutdown due to an unrelated issue.
  • the data shows that the lactate level never recovered after the shutdown.

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Abstract

La présente invention concerne un procédé permettant de réduire la concentration de microorganismes indésirables dans un courant liquide aqueux employé dans un procédé de production de sucre ou un procédé de fermentation à base de sucre, qui consiste à (a) produire du ClO2 gazeux, (b) dissoudre le ClO2 gazeux pour former une solution de ClO2, et (c) introduire la solution aqueuse de ClO2 dans le courant liquide aqueux. Un autre procédé consiste à introduire du ClO2 ayant une efficacité en tant que Clo2 d'au moins environ 90 % dans le courant liquide aqueux. L’invention concerne également un appareil pour réduire la concentration de microorganismes indésirables qui comprend un générateur de ClO2 en communication fluide avec un réservoir de charge, lui-même en communication fluide avec une cuve de fabrication de sucre ou une cuve de fermentation à base de sucre.
PCT/US2009/065737 2008-11-24 2009-11-24 Appareil et procédé pour le traitement des microorganismes dans des procédés de production de sucre et de fermentation à base de sucre WO2010060085A2 (fr)

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WO2007097874A1 (fr) * 2006-02-22 2007-08-30 Resonant Biosciences, Llc Appareil et procédé pour traiter des micro-organismes pendant leur propagation, leur conditionnement, et leur fermentation

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JPH07157386A (ja) * 1993-12-01 1995-06-20 Toyo Dynam Kk 有機性廃棄物の処理方法および処理装置
US7078201B2 (en) * 2004-12-01 2006-07-18 Burmaster Brian M Ethanol fermentation using oxidation reduction potential

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WO2007097874A1 (fr) * 2006-02-22 2007-08-30 Resonant Biosciences, Llc Appareil et procédé pour traiter des micro-organismes pendant leur propagation, leur conditionnement, et leur fermentation

Non-Patent Citations (2)

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Title
ELPHICK A: "NO NASTY FLAVOURS FROM BREWING WITH CLO2-DOSED LIQUOR" BREWING AND DISTILLING INTERNATIONAL, LONDON, GB, vol. 24, no. 4, 1 January 1993 (1993-01-01), pages 37-39, XP008078428 ISSN: 0308-1265 *
RAMIREZ-OROZCO MARTIN ET AL: "DEBARYOMYCES HANSENII GROWTH IN NONSTERILE SEAWATER CLO2-PEPTONE-CONTAINING MEDIUM DEBARYOMYCES HANSENII GROWTH IN NONSTERILE SEAWATER CLO2-PEPTONE-CONTAINING MEDIUM" CANADIAN JOURNAL OF MICROBIOLOGY, NRC RESEARCH PRESS, CA, vol. 47, no. 7, 1 July 2001 (2001-07-01), pages 676-679, XP008078385 ISSN: 0008-4166 *

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