WO2010053941A3 - Affinity displacement molecular separation - Google Patents

Affinity displacement molecular separation Download PDF

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Publication number
WO2010053941A3
WO2010053941A3 PCT/US2009/063206 US2009063206W WO2010053941A3 WO 2010053941 A3 WO2010053941 A3 WO 2010053941A3 US 2009063206 W US2009063206 W US 2009063206W WO 2010053941 A3 WO2010053941 A3 WO 2010053941A3
Authority
WO
WIPO (PCT)
Prior art keywords
ligand
proteome
molecular separation
protein
binding
Prior art date
Application number
PCT/US2009/063206
Other languages
French (fr)
Other versions
WO2010053941A2 (en
Inventor
Timothy A.J. Haystead
Original Assignee
Duke University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Duke University filed Critical Duke University
Publication of WO2010053941A2 publication Critical patent/WO2010053941A2/en
Publication of WO2010053941A3 publication Critical patent/WO2010053941A3/en

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6845—Methods of identifying protein-protein interactions in protein mixtures

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Provided herein are methods for identifying molecules that compete with a ligand for binding to a protein. The method comprises contacting a ligand with a proteome to allow protein members of the proteome to bind with the ligand, followed by the removal of the unbound ligand. At least one test molecule is added to the mixture and after passing the mixture through an ultrafiltration membrane, the filtrate is assayed for the displaced ligand. The presence of the ligand within the filtrate indicates the molecule competes with the ligand for binding to at least one protein member within the proteome.
PCT/US2009/063206 2008-11-04 2009-11-04 Affinity displacement molecular separation WO2010053941A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US11113408P 2008-11-04 2008-11-04
US61/111,134 2008-11-04

Publications (2)

Publication Number Publication Date
WO2010053941A2 WO2010053941A2 (en) 2010-05-14
WO2010053941A3 true WO2010053941A3 (en) 2010-09-10

Family

ID=42153516

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2009/063206 WO2010053941A2 (en) 2008-11-04 2009-11-04 Affinity displacement molecular separation

Country Status (1)

Country Link
WO (1) WO2010053941A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102565414B (en) * 2010-12-13 2014-01-29 天津市新药安全评价研究中心 Method for determining combination of polypeptide drug and plasma protein
CA3146258A1 (en) * 2019-09-13 2021-03-18 Eurofins Cerep Methods for using mass spectroscopy in multiplex target evaluations
CN110638818A (en) * 2019-10-11 2020-01-03 中山大学附属第一医院 Application of chloroquine or derivative hydroxychloroquine

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030138788A1 (en) * 1998-01-05 2003-07-24 Neogenesis Pharmaceuticals, Inc. Method for producing and screening mass-coded combinatorial libraries for drug discovery and target validation
US20050032243A1 (en) * 2001-07-25 2005-02-10 Pollack Gary M. Method and apparatus for rapid determination of ligand-protein binding using charcoal adsorption
US20060019410A1 (en) * 2004-07-21 2006-01-26 Qualyst, Inc. Apparatus, kits and methods for evaluating binding interactions, for detecting and quantifying binding molecules, and for sample preparation
US7179592B2 (en) * 1999-02-12 2007-02-20 Cetek Corporation Size-exclusion-based extraction of affinity ligands and active compounds from natural samples

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030138788A1 (en) * 1998-01-05 2003-07-24 Neogenesis Pharmaceuticals, Inc. Method for producing and screening mass-coded combinatorial libraries for drug discovery and target validation
US7179592B2 (en) * 1999-02-12 2007-02-20 Cetek Corporation Size-exclusion-based extraction of affinity ligands and active compounds from natural samples
US20050032243A1 (en) * 2001-07-25 2005-02-10 Pollack Gary M. Method and apparatus for rapid determination of ligand-protein binding using charcoal adsorption
US20060019410A1 (en) * 2004-07-21 2006-01-26 Qualyst, Inc. Apparatus, kits and methods for evaluating binding interactions, for detecting and quantifying binding molecules, and for sample preparation

Also Published As

Publication number Publication date
WO2010053941A2 (en) 2010-05-14

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