WO2010052342A2 - Capteur de messager chimique - Google Patents

Capteur de messager chimique Download PDF

Info

Publication number
WO2010052342A2
WO2010052342A2 PCT/EP2009/064942 EP2009064942W WO2010052342A2 WO 2010052342 A2 WO2010052342 A2 WO 2010052342A2 EP 2009064942 W EP2009064942 W EP 2009064942W WO 2010052342 A2 WO2010052342 A2 WO 2010052342A2
Authority
WO
WIPO (PCT)
Prior art keywords
cyclodextrin
conducting
electrode
anionic
substrate
Prior art date
Application number
PCT/EP2009/064942
Other languages
English (en)
Other versions
WO2010052342A3 (fr
Inventor
John Colleran
Claire Harley
Bernadette Alcock
Carmel Breslin
Niall Finnerty
Original Assignee
National University Of Ireland, Maynooth
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by National University Of Ireland, Maynooth filed Critical National University Of Ireland, Maynooth
Publication of WO2010052342A2 publication Critical patent/WO2010052342A2/fr
Publication of WO2010052342A3 publication Critical patent/WO2010052342A3/fr

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
    • G01N33/5438—Electrodes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/94—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving narcotics or drugs or pharmaceuticals, neurotransmitters or associated receptors
    • G01N33/9406—Neurotransmitters
    • G01N33/942—Serotonin, i.e. 5-hydroxy-tryptamine

Definitions

  • a sensor for the detection of chemical messengers is described herein.
  • a sensor for the detection of serotonin is reported.
  • Methods of constructing sensors according to the present invention are also described. Suitable materials for the construction of such sensors are disclosed with a view to developing a sensor capable of real-time in-vivo serotonin monitoring.
  • serotonin (1 ) plays a pivotal role as a neurotransmitter in the modulation of a myriad of physiological responses including anger, aggression, mood, sleep, sexuality, and appetite.
  • the English language abstract of Japanese Patent Publication number 9127056 describes a sensor for selectively detecting serotonin in the presence of NO and NO 2 .
  • the sensor is comprised of three carbon fibre electrodes in a single assembly wherein an operational electrode is moved up and down upon a central axis.
  • the English language abstract of Japanese Patent Publication number 3068858 discloses a simple arrangement for the electrochemical detection of serotonin. This publication is silent to the detection of serotonin the presence of interferants such as ascorbic acid.
  • CD - cyclodextrin ⁇ -CD - ⁇ -cyclodextrin
  • the present invention provides for an electrode for detecting serotonin comprising:
  • the electrode of the present invention comprises a conducting substrate.
  • the conducting substrate may comprise a metal selected from the group consisting of Pt, Ag, Au, Ru, Rh, Pd, Re, Os, Ir, Ti, Indium tin oxide (ITO) coated glass and combinations thereof.
  • the conducting substrate may comprise a non-metallic conductor such as carbon fibres, graphite, glassy carbon, diamond, carbon paste and pyrolithic carbon electrodes, or boron doped diamond.
  • the conducting substrate comprises Au.
  • the conducting polymer is a biocompatible conducting polymer.
  • biocompatible is a reference to materials that are non-toxic to biological tissues.
  • the conducting polymer of the electrode of the present invention may be selected from the group consisting of polythiophenes, polypyrroles and combinations thereof. Desirably, the conducting polymer comprises a polythiophene. Suitably, the conducting polymer comprises PEDOT [polyethylenedioxythiophene] (6) wherein n ⁇ 1.
  • the cyclodextrin macrocycle comprises an anionic cyclodextrin macrocycle.
  • the anionic cyclodextrin macrocycle comprises an anionic ⁇ -cyclodextrin, ⁇ -cyclodextrin, ⁇ -cyclodextrin and combinations thereof.
  • the anionic cyclodextrin macrocycle comprises an anionic ⁇ - cyclodextrin.
  • the cyclodextrin macrocycle of the electrode of the present invention is anionic (negatively charged).
  • metabolites known to poison prior art electrodes for example DOPAC and homovanillic acid
  • the electrode construction of the present invention should not be affected by these metabolites to the same extent, as the anionic cyclodextrin should repel these anionic metabolites.
  • the anionic cyclodextrin macrocycle comprises a sulfonated cyclodextrin macrocycle.
  • the sulfonated cyclodextrin macrocycle may comprise a sulfonated ⁇ -cyclodextrin, a sulfonated ⁇ -cyclodextrin, a sulfonated v- cyclodextrin and combinations thereof.
  • the anionic cyclodextrin macrocycle comprises a sulfonated ⁇ -cyclodextrin.
  • sulfonated cyclodextrin macrocycle refers to any cyclodextrin wherein one or more of the hydroxy groups of the glucopyranoside rings are sulfonated.
  • the term is sometimes used interchangeably with sulfated cyclodextrin.
  • sulfated cyclodextrin and sulfonated cyclodextrin are to be interpreted as one in the same provided the definition above is satisfied, i.e. having one or more of the hydroxy groups of the glucopyranoside rings sulfonated.
  • sulfonated ⁇ -cyclodextrin (7) is commercially available from Sigma-Alrich ® as sulfated ⁇ -cyclodextrin.
  • the electrode of the present invention may further comprise a deposit of a halogenated material on the polymer material.
  • Suitable halogenated materials include fluorinated or chlorinated materials, such as perfluoro, perchloro and perfluorochloro polymers.
  • halogenated ionomers such as a sulfonated tetrafluorethylene copolymer.
  • Nafion® is a commercially available material by DuPont. Nafion® may be considered a tetrafluoroethylene-perfluoro-3,6-dioxa-4- methyl-7-octenesulfonic acid copolymer (see for example CAS 31 175-20-9).
  • the deposit of the halogenated material may form a coating around the electrode.
  • the invention extends to a method of preparing an electrode for detecting serotonin comprising:
  • the monomeric precursor to a conducting polymer comprises a monomeric precursor to a polythiophene, a monomeric precursor to a polypyrrole and combinations thereof. Further desirably, the monomeric precursor to a conducting polymer comprises a monomeric precursor to a polythiophene. Preferably, the monomeric precursor to a conducting polymer comprises ethylenedioxythiophene (EDOT), a precursor to polyethylenedioxythiophene (PEDOT).
  • EDOT ethylenedioxythiophene
  • PEDOT polyethylenedioxythiophene
  • the anionic cyclodextrin is incorporated into the PEDOT during polymerisation as a counter ion in order to neutralise the positive charge formed on the PEDOT chain during the oxidation of the monomer.
  • the substrate comprises a conducting substrate.
  • the conducting substrate may comprise a metal selected from the group consisting of Pt, Ag, Au, Ru, Rh, Pd, Re, Os, Ir, Ti, Indium tin oxide (ITO) coated glass and combinations thereof.
  • the conducting substrate may comprise a non- metallic conductor such as carbon fibres, graphite, glassy carbon, diamond, carbon paste and pyrolithic carbon electrodes, or boron doped diamond.
  • the conducting substrate comprises Au.
  • the cyclodextrin macrocycle comprises an anionic cyclodextrin macrocycle.
  • the anionic cyclodextrin macrocycle comprises an anionic ⁇ -cyclodextrin, ⁇ -cyclodextrin, ⁇ -cyclodextrin and combinations thereof.
  • the anionic cyclodextrin macrocycle comprises an anionic ⁇ -cyclodextrin.
  • the anionic cyclodextrin macrocycle comprises a sulfonated cyclodextrin macrocycle.
  • the sulfonated cyclodextrin macrocycle may comprise a sulfonated ⁇ -cyclodextrin, a sulfonated ⁇ -cyclodextrin, a sulfonated ⁇ -cyclodextrin and combinations thereof.
  • the anionic cyclodextrin macrocycle comprises a sulfonated ⁇ - cyclodextrin.
  • the step of applying an electrical potential may comprise potentiostatic methods, and potentiodynamic methods such as cyclic voltammetry and combinations thereof.
  • the method of the present invention may further comprise the step of: (v) applying a solution of a halogenated material to the electrode.
  • the electrode may be coated with a solution of the halogenated material.
  • the electrode may be dipped into a solution of the halogenated material.
  • the halogenated material my be applied using the dip-coating or pre-coating methods described in the detailed description of the invention.
  • Suitable halogenated materials include fluorinated or chlorinated materials, such as perfluoro, perchloro and perfluorochloro polymers.
  • halogenated ionomers such as a sulfonated tetrafluorethylene copolymer.
  • a sulfonated tetrafluorethylene copolymer is one suitable commercially available material.
  • Nafion® may be considered a tetrafluoroethylene-perfluoro-3,6-dioxa-4-methyl-7-octenesulfonic acid copolymer (see for example CAS 31 175-20-9).
  • the present invention further provides for a sensor for selective detection of serotonin in the presence of dopamine, epinephrine, norepinephrine, ascorbic acid and combinations thereof comprising: an electrode comprising:
  • a conducting or semi-conducting substrate (i) a conducting or semi-conducting substrate; and (ii) a polymer material on said substrate, wherein said polymer comprises a conducting polymer doped with a sulfonated ⁇ -cyclodextrin macrocycle.
  • ascorbic acid comprises neutral ascorbic acid and the anionic derivative ascorbate.
  • ascorbic acid when dissolved in water will dissociate into ascorbate anions and protons (in an amount proportional to the pKa of ascorbic acid).
  • the senor of the present invention comprises a conducting substrate.
  • the conducting substrate may comprise a metal selected from the group consisting of Pt, Ag, Au, Ru, Rh, Pd, Re, Os, Ir, Ti, Indium tin oxide (ITO) coated glass and combinations thereof.
  • the conducting substrate may comprise a non-metallic conductor such as carbon fibres, graphite, glassy carbon, diamond, carbon paste and pyrolithic carbon electrodes, or boron doped diamond.
  • the conducting substrate comprises
  • the conducting polymer is a biocompatible conducting polymer.
  • biocompatible is a reference to materials that are non-toxic to biological tissues.
  • the conducting polymer of the sensor of the present invention may be selected from the group consisting of polythiophene materials, polypyrrole materials and combinations thereof. Desirably, the conducting polymer comprises a polythiophene material. Suitably, the conducting polymer comprises PEDOT
  • the electrode of the sensor of the present invention may further comprise a deposit of a halogenated material on the polymer material.
  • Suitable halogenated materials included fluorinated or chlorinated materials, such as perfluoro, perchloro and perfluorochloro polymers.
  • halogenated ionomers such as a sulfonated tetrafluorethylene copolymer.
  • Nafion® is a tetrafluoroethylene-perfluoro-3,6- dioxa-4-methyl-7-octenesulfonic acid copolymer (see for example CAS 31 175-20-9).
  • the deposit of the halogenated material may form a coating around the electrode.
  • the electrode and sensor of the present invention provide for detecting serotonin in solution.
  • solution comprises bodily fluids such as plasma, blood, extra-cellular fluid, etc. having serotonin dissolved therein.
  • the electrodes utilised in detecting serotonin levels have the potential to be miniaturised and conveniently placed in the living organism to give in- vivo data at the sub-second timescale.
  • the electrode and sensor of the present invention may optimally sense serotonin at concentrations below 60 ⁇ M.
  • the electrode and sensor of the present invention may optimally sense serotonin at concentrations below 40 ⁇ M. At serotonin concentrations above these values the sensitivity of the electrode and sensor may decrease.
  • the electrode and sensor of the present invention provide for real-time measurement of serotonin, both in-vivo and in-vitro. Further still, the electrode and sensor of the present invention for detecting serotonin has the potential for in-situ monitoring. [0040] Potential applications of the electrode and sensor of the present invention include the evaluation of test compounds on serotonin concentrations in the brain, and the resulting neurological response.
  • Figure 1 illustrates electropolymerisation of PEDOT/cyclodextrin film at a Gold electrode according to the present invention. Potential cycled from -0.5 to +1.08 V vs
  • Figure 2 illustrates the cyclic voltammetric response of the PEDOT/sulfonated ⁇ -CD film on a gold electrode according to the present invention to 5x10 "5 M 5-HT and a mixture of 5x10 "5 M 5-HT & 5x10 "4 M AA.
  • Figure 3 depicts the cyclic voltammetric response of the PEDOT/sulfonated ⁇ -
  • CD film on a gold electrode according to the present invention to 5x10 "5 M 5-HT & DA, and a mixture of 5x10 "5 M 5-HT, DA & AA.
  • Figure 4 depicts the cyclic voltammetric response of the PEDOT/sulfonated ⁇ -
  • CD film on a gold electrode according to the present invention to a mixture of 5-HT, AA,
  • Figure 5 illustrates the current response of 5.0 x10 "6 M 5-HT at the Au microelectrode using cyclic voltammetry.
  • Figure 6 illustrates the current response of AA using constant potential amperometry at a polymer modified platinum (Pt) microlectrode in the presence and absence of Nafion®.
  • Figure 7 illustrates the current response of 5-HT using constant potential amperometry at a polymer modified platinum (Pt) microlectrode in the presence and absence of Nafion®.
  • Cyclodextrins have been used in place of surfactants, 4 ' 5 owing to the ability of cyclodextrins to form a host guest interaction with EDOT, thus increasing the solubility of the EDOT monomer in water.
  • sulfonated ⁇ - cyclodextrin ( ⁇ -CD) was utilised as the dopant anion necessary for film formation to occur.
  • the films were electropolymerised onto gold electrodes from an aqueous solution of 0.1 M ethylenedioxythiophene and 0.01 M sulfonated ⁇ -cyclodextrin, sodium salt. Polymerisation was carried out by cycling the potential between -0.5 and 1.08
  • V/SCE at a scan rate of 50 mV s "1 for a total of three cycles.
  • the PEDOT/sulfonated ⁇ -cyclodextrin film properties vary depending on: a) the EDOT:sulfonated ⁇ -CD solution concentrations (and ratio); and b) the polymerisation technique utilised - when cyclic voltammetry is utilised the following parameters can be modified to vary the PEDOT/sulfonated ⁇ - cyclodextrin film properties; i) the upper (anodic) potential of the voltammetric sweep used when fabricating the polymer film. This upper potential is important for system optimisation; and ii) the sweep rate.
  • the conditions resulting in optimal serotonin sensing comprise:
  • the electrode array of the present invention should be capable of detecting 5-HT in the presence of DA.
  • Figure 3 shows the cyclic voltammetric response of the PEDOT/sulfonated ⁇ -CD film on a gold electrode according to the present invention to 5x10 "5 M 5-HT & DA (lower trace 301 ), and a mixture of 5x10 "5 M 5-HT, DA & AA (upper trace 302).
  • DA Dopamine
  • 5-HT Serotonin
  • the electrode comprising the PEDOT/sulfonated ⁇ -CD film according to the present invention was capable of selectively detecting all three species as independent peaks.
  • FIG. 4 shows the cyclic voltammetric response of the PEDOT/sulfonated ⁇ -CD film on a gold electrode according to the present invention to a mixture of 1.67x10 "5 M 5-HT, 2x10 "4 M AA, 2x10 "5 M DA, 1x10 "5 M EP and 6.67x10 "6 M norEP.
  • the signals labelled 401 at -0.035V, -0.15V & -0.5V correspond to 5-HT.
  • Signal 402 at -0.2V is from ascorbic acid, and signal 403 at -0.42V is a mixture of DA, EP and norEP.
  • FIG. 4 clearly illustrates that the gold electrode comprising the PEDOT/sulfonated ⁇ -CD film according to the present invention is capable of selectively detecting 5-HT in the presence of AA, DA, EP and norEP.
  • the microelectrode should have a diameter less than 200 ⁇ m.
  • the microelectrode selected should have a diameter of less than 180 ⁇ m. References to diameter are inclusive of any additional layers or deposits applied to the electrode surface.
  • a modified gold microelectrode having a diameter of 75 ⁇ m (0.075 mm) was prepared as follows:
  • a PEDOT/sulfonated ⁇ -cyclodextrin film was electropolymerised onto a gold electrode of 75 ⁇ m diameter from an aqueous solution of 0.1 M ethylenedioxythiophene and 0.01
  • Figure 5 illustrates the current response of 5.0 x10 "6 M 5-HT at the Au microelectrode using cyclic voltammetry.
  • the full sweep of the cyclic voltammegram is shown in the top right hand corner.
  • the main image is an exploded view of the region of the cyclic voltammegram showing the current response 501 of the 5-HT.
  • the peak 501 corresponding to oxidation of 5-HT is observed between 0.40 V and 0.50 V.
  • a PEDOT/sulfonated ⁇ -cyclodextrin film was coated on to a platinum (Pt) microelectrode using the same method disclosed for the gold microelectrode supra.
  • Pt platinum
  • Nafion® a perfluorinated polymer, may be applied to electrodes in order to establish selectivity against electroactive interferents. Methods of doing this include the dip-coat method and the pre-coat method.
  • the pre-coat method involves placing a fixed volume (e.g. 5 ml) of Nafion® onto a watch glass using a syringe.
  • the Nafion® droplet is allowed to air dry at room temperature for 5 minutes.
  • the solvent 5% Nafion® dissolved in aliphatic alcohols
  • further individual droplets are placed on top of the original droplet using the same procedures previously outlined for the initial drop. What results from solvent evaporation is a concentrated layer of Nafion® on the watch glass.
  • a final drop of Nafion® is placed onto the concentrated pre-coat of Nafion.
  • the active surface of the electrode is then dipped into the Nafion® concentrated layer.
  • the electrode is then removed immediately from the concentrated layer of Nafion® and let air dry at room temperature for 2 minutes.
  • the purpose of the fresh Nafion® droplet is to adhere the concentrated Nafion® layer to the electrode.
  • the electrode may optionally be annealed to help drying.
  • the electrode can be coated again using the same procedure if desired. This pre-coat fabrication can be carried out numerous times.
  • FIG. 6 illustrates the current response of AA at the Pt PEDOT/sulfonated ⁇ - cyclodextrin modified microelectrode using constant potential amperometry. Concentration of AA is plotted on the X-axis and Current (I) measured is plotted on the Y-axis.
  • Trace 601 illustrates the response of AA at the Pt-PEDOT/sulfonated ⁇ - cyclodextrin microelectrode. The current measured increases with increasing AA concentration.
  • Trace 602 illustrates the response of AA at the Pt-PEDOT/sulfonated ⁇ - cyclodextrin/Nafion® microelectrode. In the presence of Nafion® minimal AA is oxidised at the electrode, thus no change in current is registered. At high AA concentrations a very low current is observed. Thus indicating that National® is hindering oxidation of AA at the electrode.
  • Figure 7 illustrates the current response of 5-HT at the Pt PEDOT/sulfonated ⁇ - cyclodextrin modified microelectrode using constant potential amperometry. Concentration of 5-HT is plotted on the X-axis and Current (I) measured is plotted on the Y-axis.
  • Trace 701 illustrates the response of 5-HT at the Pt-PEDOT/sulfonated ⁇ - cyclodextrin microelectrode.
  • 5-HT concentrations above 60 ⁇ M result in no change in the current measured.
  • a threshold 5-HT concentration value has been reached, beyond which no further oxidation of 5-HT at the electrode is observable.
  • Trace 602 illustrates the response of 5-HT at the Pt-PEDOT/sulfonated ⁇ - cyclodextrin/Nafion® microelectrode.
  • a lower threshold 5-HT concentration of 40 ⁇ M is observable.
  • 5-HT concentrations greater than 40 ⁇ M the measured current appears to decrease.
  • In-vivo concentrations of 5-HT are significantly lower than 40 ⁇ M and the issues surrounding the threshold concentration values of 5-HT should not be problematic.
  • the Nafion/PEDOT/ sulfonated ⁇ - cyclodextrin modified microelectrode shows efficient qualities for the detection of 5-HT.
  • Microelectrode analyses were performed utilising AD Instruments Powerlab 8/30 in conjunction with an ACM Four Channel Biostat.

Abstract

L'invention porte sur un capteur pour la détection de messagers chimiques, plus particulièrement sur un capteur pour la détection de la sérotonine. La sérotonine joue un rôle central en tant que neurotransmetteur dans la modulation d'une myriade de réponses physiologiques dont la peur, l'agression, l'humeur, le sommeil, la sexualité et l'appétit. Une électrode pour détecter la sérotonine comprend un substrat conducteur ou semi-conducteur, et un matériau polymère sur ledit substrat. Ledit polymère comprend un polymère conducteur dopé par un macrocycle de cyclodextrine. Des matériaux polymères appropriés comprennent des polypyrroles et des polythiophènes, par exemple, PEDOT. Des macrocycles de cyclodextrine appropriés comprennent des macrocycles de cyclodextrine anioniques, par exemple des β-cyclodextrines sulfonées (CD). L'invention porte également sur un capteur capable de détecter sélectivement la sérotonine en présence d'acide ascorbique, d'épinéphrine, de norépinéphrine et de dopamine.
PCT/EP2009/064942 2008-11-10 2009-11-10 Capteur de messager chimique WO2010052342A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IES20080900 IES20080900A2 (en) 2008-11-10 2008-11-10 Chemical messenger sensor
IES2008/0900 2008-11-10

Publications (2)

Publication Number Publication Date
WO2010052342A2 true WO2010052342A2 (fr) 2010-05-14
WO2010052342A3 WO2010052342A3 (fr) 2010-07-15

Family

ID=42107372

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2009/064942 WO2010052342A2 (fr) 2008-11-10 2009-11-10 Capteur de messager chimique

Country Status (2)

Country Link
IE (1) IES20080900A2 (fr)
WO (1) WO2010052342A2 (fr)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106353376A (zh) * 2016-08-30 2017-01-25 南京师范大学 一种β‑肾上腺受体激动剂多残留检测电化学传感器及其检测方法
CN107923865A (zh) * 2015-09-01 2018-04-17 株式会社源医疗 利用电化学检测方法的过敏原检测装置

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5587466A (en) * 1993-06-03 1996-12-24 Commissariat A L'energie Atomique Conductive polymer doped by a sulphonated cyclodextrin salt and device for obtaining and/or supplying an active substance incorporating said polymer
US20030027239A1 (en) * 2000-05-16 2003-02-06 Schaffar Berhard Peter Harald Creatinine biosensor
US6582583B1 (en) * 1998-11-30 2003-06-24 The United States Of America As Represented By The Department Of Health And Human Services Amperometric biomimetic enzyme sensors based on modified cyclodextrin as electrocatalysts
WO2006127023A2 (fr) * 2004-08-24 2006-11-30 University Of South Florida Membrane polymere amelioree par resine epoxyde, pour augmenter la longevite de capteurs biologiques

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5587466A (en) * 1993-06-03 1996-12-24 Commissariat A L'energie Atomique Conductive polymer doped by a sulphonated cyclodextrin salt and device for obtaining and/or supplying an active substance incorporating said polymer
US6582583B1 (en) * 1998-11-30 2003-06-24 The United States Of America As Represented By The Department Of Health And Human Services Amperometric biomimetic enzyme sensors based on modified cyclodextrin as electrocatalysts
US20030027239A1 (en) * 2000-05-16 2003-02-06 Schaffar Berhard Peter Harald Creatinine biosensor
WO2006127023A2 (fr) * 2004-08-24 2006-11-30 University Of South Florida Membrane polymere amelioree par resine epoxyde, pour augmenter la longevite de capteurs biologiques

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
BIDAN G ET AL: "Incorporation of sulphonated cyclodextrins into polypyrrole: An approach for the electro-controlled delivering of neutral drugs" BIOSENSORS AND BIOELECTRONICS, vol. 10, no. 1-2, 1995, pages 219-229, XP002579616 ISSN: 0956-5663 *
LAGROST ET AL: "Host-guest complexation: a new strategy for electrodeposition of processable polythiophene composites from aqueous medium" CHEMICAL COMMUNICATIONS - CHEMCOM, ROYAL SOCIETY OF CHEMISTRY, GB LNKD- DOI:10.1039/A707862E, no. 4, 1 January 1998 (1998-01-01), pages 489-490, XP002144876 ISSN: 1359-7345 *
VASANTHA V S ET AL: "Effect of hydroxypropyl-[beta]-cyclodextrin on the electrochemical oxidation and polymerization of 3,4-ethylenedioxythiophene" JOURNAL OF ELECTROANALYTICAL CHEMISTRY 20020222 ELSEVIER NL LNKD- DOI:10.1016/S0022-0728(01)00748-3, vol. 520, no. 1-2, 22 February 2002 (2002-02-22), pages 79-88, XP002579617 *

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107923865A (zh) * 2015-09-01 2018-04-17 株式会社源医疗 利用电化学检测方法的过敏原检测装置
EP3346264A4 (fr) * 2015-09-01 2018-08-08 Wonmedical Corp. Appareil de détection d'allergènes à l'aide d'un procédé de détection électrochimique
JP2018526659A (ja) * 2015-09-01 2018-09-13 株式会社ウォンメディカル 電気化学的検出方法によるアレルゲン検出装置
CN106353376A (zh) * 2016-08-30 2017-01-25 南京师范大学 一种β‑肾上腺受体激动剂多残留检测电化学传感器及其检测方法

Also Published As

Publication number Publication date
IES20080900A2 (en) 2010-05-12
WO2010052342A3 (fr) 2010-07-15

Similar Documents

Publication Publication Date Title
Emir et al. Amperometric nonenzymatic glucose biosensor based on graphite rod electrode modified by Ni-nanoparticle/polypyrrole composite
Kim et al. Continuous glucose monitoring using a microneedle array sensor coupled with a wireless signal transmitter
Pappa et al. Organic electronics for point-of-care metabolite monitoring
US10492708B1 (en) Tissue-penetrating electrochemical sensor featuring a co-electrodeposited thin film comprised of polymer and bio-recognition element
Uang et al. Fabrication of glucose oxidase/polypyrrole biosensor by galvanostatic method in various pH aqueous solutions
Harreither et al. Carbon nanotube fiber microelectrodes show a higher resistance to dopamine fouling
US9309550B2 (en) Analyte sensors having nanostructured electrodes and methods for making and using them
Ates et al. Carbon fiber microelectrodes electrocoated with polycarbazole and poly (carbazole-co-p-tolylsulfonyl pyrrole) films for the detection of dopamine in presence of ascorbic acid
Çetin et al. An amperometric glucose biosensor based on PEDOT nanofibers
EP2492351B1 (fr) Détecteur électrochimique pour la détection d'analytes dans des milieux liquides
Yang et al. Glucose sensor using a microfabricated electrode and electropolymerized bilayer films
Park et al. Glucose biosensor constructed from capped conducting microtubules of PEDOT
Shi et al. An ascorbic acid amperometric sensor using over-oxidized polypyrrole and palladium nanoparticles composites
Barsan et al. New CNT/poly (brilliant green) and CNT/poly (3, 4-ethylenedioxythiophene) based electrochemical enzyme biosensors
Thakur et al. Ultrasensitive electrochemical biosensors for dopamine and cholesterol: recent advances, challenges and strategies
CN111693588B (zh) 柔性可植入纤维状有机电化学晶体管及其制备方法
Wang et al. Glassy carbon electrode modified with poly (dibromofluorescein) for the selective determination of dopamine and uric acid in the presence of ascorbic acid
Smith et al. Quantitative comparison of enzyme immobilization strategies for glucose biosensing in real‐time using fast‐scan cyclic voltammetry coupled with carbon‐fiber microelectrodes
Hernández‐Jiménez et al. Voltammetric Determination of Metronidazole Using a Sensor Based on Electropolymerization of α‐Cyclodextrin over a Carbon Paste Electrode
Sriprasertsuk et al. Polypyrrole-coated carbon fibre electrodes for paracetamol and clozapine drug sensing
Cho et al. Timed electrodeposition of PEDOT: Nafion onto carbon fiber-microelectrodes enhances dopamine detection in zebrafish retina
KR20180006835A (ko) 바이오 센서 및 그의 제작 방법
US20100116655A1 (en) Chemical messenger sensor
Aldea et al. Gold coated electrospun polymeric fibres as new electrode platform for glucose oxidase immobilization
Chung et al. Reproducible fabrication of miniaturized glucose sensors: preparation of sensing membranes for continuous monitoring

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 09748797

Country of ref document: EP

Kind code of ref document: A2

NENP Non-entry into the national phase in:

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 09748797

Country of ref document: EP

Kind code of ref document: A2