WO2010049753A3 - A protein extraction buffer, a kit comprising it and method of its use - Google Patents

A protein extraction buffer, a kit comprising it and method of its use Download PDF

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Publication number
WO2010049753A3
WO2010049753A3 PCT/IB2008/003870 IB2008003870W WO2010049753A3 WO 2010049753 A3 WO2010049753 A3 WO 2010049753A3 IB 2008003870 W IB2008003870 W IB 2008003870W WO 2010049753 A3 WO2010049753 A3 WO 2010049753A3
Authority
WO
WIPO (PCT)
Prior art keywords
kit
extraction buffer
protein extraction
buffer
formalin
Prior art date
Application number
PCT/IB2008/003870
Other languages
French (fr)
Other versions
WO2010049753A2 (en
Inventor
Elias Georges
Corinne Benquet
Julie Lanthier
Original Assignee
Aurelium Biopharma Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Aurelium Biopharma Inc. filed Critical Aurelium Biopharma Inc.
Priority to PCT/IB2008/003870 priority Critical patent/WO2010049753A2/en
Publication of WO2010049753A2 publication Critical patent/WO2010049753A2/en
Publication of WO2010049753A3 publication Critical patent/WO2010049753A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/145—Extraction; Separation; Purification by extraction or solubilisation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/4055—Concentrating samples by solubility techniques
    • G01N2001/4061—Solvent extraction

Abstract

Disclosed are detergent-free protein extract buffers to extract proteins from formalin- fixed paraffin-embedded tissue sections, kit comprising the buffer, and methods for using the buffer to extract proteins from formalin- fixed, paraffin-embedded tissue sections.
PCT/IB2008/003870 2008-10-31 2008-10-31 A protein extraction buffer, a kit comprising it and method of its use WO2010049753A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PCT/IB2008/003870 WO2010049753A2 (en) 2008-10-31 2008-10-31 A protein extraction buffer, a kit comprising it and method of its use

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/IB2008/003870 WO2010049753A2 (en) 2008-10-31 2008-10-31 A protein extraction buffer, a kit comprising it and method of its use

Publications (2)

Publication Number Publication Date
WO2010049753A2 WO2010049753A2 (en) 2010-05-06
WO2010049753A3 true WO2010049753A3 (en) 2010-06-24

Family

ID=42129368

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB2008/003870 WO2010049753A2 (en) 2008-10-31 2008-10-31 A protein extraction buffer, a kit comprising it and method of its use

Country Status (1)

Country Link
WO (1) WO2010049753A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101985461A (en) * 2010-09-19 2011-03-16 生工生物工程(上海)有限公司 Method for extracting total proteins from formaldehyde-fixed tissues or formaldehyde-fixed paraffin-embedded tissues

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007062270A2 (en) * 2005-11-28 2007-05-31 Proteomtech Inc. Methods for production of recombinant alpha1-antitrypsin
WO2008054592A2 (en) * 2006-09-29 2008-05-08 Proteomtech, Inc. Methods for production of recombinant plasminogen and plasmin polypeptides

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007062270A2 (en) * 2005-11-28 2007-05-31 Proteomtech Inc. Methods for production of recombinant alpha1-antitrypsin
WO2008054592A2 (en) * 2006-09-29 2008-05-08 Proteomtech, Inc. Methods for production of recombinant plasminogen and plasmin polypeptides

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
FUNKENSTEIN, B. ET AL.: "Expression, purification, renaturation and activation of fish myostatin expressed in Escherichia coli: Facilitation of refolding and activity inhibition by myostatin prodomain", PROT. EXPR. PURIF., vol. 54, July 2007 (2007-07-01), pages 54 - 65 *
IKEDA, K. ET AL.: "Extraction and analysis of diagnostically useful proteins from formalin-fixed, paraffin-embedded tissue sections", J. HISTOCHEM. CYTOCHEM., vol. 46, no. 3, March 1998 (1998-03-01), pages 397 - 403 *
JIANG, X. ET AL.: "Development of efficient protein extraction methods for shotgun proteome analysis of formalin-fixed tissues", J. PROTEOME RES., vol. 6, March 2007 (2007-03-01), pages 1038 - 1047 *

Also Published As

Publication number Publication date
WO2010049753A2 (en) 2010-05-06

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