WO2010046377A3 - Immunoactivating conjugates comprising nanoparticles coated with peptides - Google Patents

Immunoactivating conjugates comprising nanoparticles coated with peptides Download PDF

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Publication number
WO2010046377A3
WO2010046377A3 PCT/EP2009/063777 EP2009063777W WO2010046377A3 WO 2010046377 A3 WO2010046377 A3 WO 2010046377A3 EP 2009063777 W EP2009063777 W EP 2009063777W WO 2010046377 A3 WO2010046377 A3 WO 2010046377A3
Authority
WO
WIPO (PCT)
Prior art keywords
immune system
conjugates
activation
innate immune
peptides
Prior art date
Application number
PCT/EP2009/063777
Other languages
French (fr)
Other versions
WO2010046377A2 (en
Inventor
Víctor FRANCO PUNTES
Neus GÓMEZ BASTÚS
Ester SÁNCHEZ TILLÓ
Consol Farrera Sinfreu
Antonio Celada Cotarelo
Jorge Lloberas Cavero
Ernest GIRALT LLEDÓ
Silvia PUJALS RIATÓS
Original Assignee
Fundació Privada Institut Català De Nanotecnologia
Institució Catalana De Recerca I Estudis Avançats
Universidad De Barcelona
Fundació Privada Institut De Recerca Biomèdica
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Fundació Privada Institut Català De Nanotecnologia, Institució Catalana De Recerca I Estudis Avançats, Universidad De Barcelona, Fundació Privada Institut De Recerca Biomèdica filed Critical Fundació Privada Institut Català De Nanotecnologia
Publication of WO2010046377A2 publication Critical patent/WO2010046377A2/en
Publication of WO2010046377A3 publication Critical patent/WO2010046377A3/en

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Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
    • A61K47/69—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit
    • A61K47/6921—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit the form being a particulate, a powder, an adsorbate, a bead or a sphere
    • A61K47/6923—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit the form being a particulate, a powder, an adsorbate, a bead or a sphere the form being an inorganic particle, e.g. ceramic particles, silica particles, ferrite or synsorb
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Veterinary Medicine (AREA)
  • General Health & Medical Sciences (AREA)
  • Epidemiology (AREA)
  • Ceramic Engineering (AREA)
  • Inorganic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Organic Chemistry (AREA)
  • Medicinal Preparation (AREA)
  • Peptides Or Proteins (AREA)

Abstract

The present invention relates to a conjugate having colloidal stability in a medium comprising a metallic nanoparticle coated with a non-activating peptide which is ordered on the nanoparticle surface. It also relates to a pharmaceutical composition and to a process for the preparation thereof. The conjugates of the invention are used for the activation of the immune system, in particular the innate immune system, as well as for the modulation of immune responses, production of antibodies and detection of substances via antigen-antibody interactions. Inventors have found that non-activating peptides which, in themselves, do not trigger any activation of the immune system, even when they are in an aggregated state, are capable to activate the immune system, in particular the innate immune system, when ordered onto the surface of a metallic nanoparticle. These conjugates are able to achieve activation of the innate immune system, in particular the innate immune system despite their small size. Thus, the use of the conjugates of the invention offers the possibility to render molecules, otherwise undetectable, visible to the immune system.
PCT/EP2009/063777 2008-10-23 2009-10-21 Immunoactivating conjugates comprising nanoparticles coated with peptides WO2010046377A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP08382058 2008-10-23
EP08382058.9 2008-10-23

Publications (2)

Publication Number Publication Date
WO2010046377A2 WO2010046377A2 (en) 2010-04-29
WO2010046377A3 true WO2010046377A3 (en) 2010-12-16

Family

ID=40377611

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2009/063777 WO2010046377A2 (en) 2008-10-23 2009-10-21 Immunoactivating conjugates comprising nanoparticles coated with peptides

Country Status (1)

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WO (1) WO2010046377A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ITTO20110561A1 (en) * 2011-06-27 2012-12-28 Consiglio Nazionale Ricerche VECTOR FOR THE RELEASE OF AN ACTIVE AGENT, PHOTOSHIPABLE FOR RADIATION IN THE VISIBLE
CN115737677A (en) * 2022-07-25 2023-03-07 天津医科大学总医院 Application of polypeptide-modified gold nanoparticles in preparation of medicine for inhibiting diabetic retinopathy

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007063300A2 (en) * 2005-11-29 2007-06-07 Imperial Innovations Limited Particles
WO2007069068A2 (en) * 2005-12-16 2007-06-21 Diatos Cell penetrating peptide conjugates for delivering nucleic acids into cells

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007063300A2 (en) * 2005-11-29 2007-06-07 Imperial Innovations Limited Particles
WO2007069068A2 (en) * 2005-12-16 2007-06-21 Diatos Cell penetrating peptide conjugates for delivering nucleic acids into cells

Non-Patent Citations (11)

* Cited by examiner, † Cited by third party
Title
BASTUS ET AL: "Gold nanoparticles for selective and remote heating of beta-amyloid protein aggregates", MATERIALS SCIENCE AND ENGINEERING C, vol. 27, no. 5-8, 8 August 2007 (2007-08-08), pages 1236 - 1240, XP022190479, ISSN: 0928-4931 *
BECKER CHRISTIAN F W ET AL: "Functional immobilization of the small GTPase Rab6A on DNA-gold nanoparticles by using a site-specifically attached poly(ethylene glycol) linker and thiol place-exchange reaction", CHEMBIOCHEM - A EUROPEAN JOURNAL OF CHEMICAL BIOLOGY, vol. 8, no. 1, 1 January 2007 (2007-01-01), pages 32 - 36, XP009105104, ISSN: 1439-4227 *
CONTARINO MARK R ET AL: "Modular, self-assembling peptide linkers for stable and regenerable carbon nanotube biosensor interfaces", JOURNAL OF MOLECULAR RECOGNITION, vol. 19, no. 4, July 2006 (2006-07-01), pages 363 - 371, XP002517850, ISSN: 0952-3499 *
KELLY D F ET AL: "Identification of the beta1-integrin binding site on alpha-actinin by cryoelectron microscopy", JOURNAL OF STRUCTURAL BIOLOGY, vol. 149, no. 3, 1 March 2005 (2005-03-01), pages 290 - 302, XP004757128, ISSN: 1047-8477 *
KIM ET AL: "Protein kinase assay on peptide-conjugated gold nanoparticles", BIOSENSORS & BIOELECTRONICS, vol. 23, no. 7, 12 October 2007 (2007-10-12), pages 980 - 986, XP022440000, ISSN: 0956-5663 *
LEVY R ET AL: "Rational and combinatorial design of peptide capping ligands for gold nanoparticles", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, vol. 126, no. 32, 18 August 2004 (2004-08-18), pages 10076 - 10084, XP002332958, ISSN: 0002-7863 *
LIU Y ET AL: "Synthesis, stability and cellular internalization of gold nanoparticles containing mixed peptide-poly(ethylene glycol) monolayers", ANALYTICAL CHEMISTRY, vol. 79, 9 February 2007 (2007-02-09), pages 2221 - 2229, XP008079811 *
MO X ET AL: "Synthesis and application of gold nanoparticles functionalized with collagen mimetic peptides", MATERIALS RESEARCH SOCIETY SYMPOSIUM PROCEEDINGS, vol. 845, 2005, pages 143 - 147, XP002517849 *
MO X ET AL: "Synthesis and application of gold nanoparticles functionalized with collagen mimetic peptides", SYMPOSIUM NANOSCALE MATERIALS SCIENCE IN BIOLOGY AND MEDICINE, vol. 845, 28 November 2004 (2004-11-28) - 2 December 2004 (2004-12-02), pages 143 - 147 *
TKACHENKO ALEXANDER ET AL: "Assembly and characterization of biomolecule-gold nanoparticle conjugates and their use in intracellular imaging.", METHODS IN MOLECULAR BIOLOGY, vol. 303, 2005, pages 85 - 99, XP008103152, ISSN: 1064-3745 *
WANG ZHENXIN ET AL: "The peptide route to multifunctional gold nanoparticles", BIOCONJUGATE CHEMISTRY, vol. 16, no. 3, 1 May 2005 (2005-05-01), pages 497 - 500, XP002332959, ISSN: 1043-1802 *

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Publication number Publication date
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