WO2010043418A3 - Integrierte amplifikation, prozessierung und analyse von biomolekülen in einem mikrofluidischen reaktionsträger - Google Patents
Integrierte amplifikation, prozessierung und analyse von biomolekülen in einem mikrofluidischen reaktionsträger Download PDFInfo
- Publication number
- WO2010043418A3 WO2010043418A3 PCT/EP2009/007477 EP2009007477W WO2010043418A3 WO 2010043418 A3 WO2010043418 A3 WO 2010043418A3 EP 2009007477 W EP2009007477 W EP 2009007477W WO 2010043418 A3 WO2010043418 A3 WO 2010043418A3
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- WO
- WIPO (PCT)
- Prior art keywords
- amplification
- biomolecules
- analysis
- processing
- reaction medium
- Prior art date
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
- C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
- C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Die Erfindung bezieht sich auf eine Kombination von Amplifϊkationsprozessen eines Probenmaterials mit den in der Patentanmeldung "IMPROVED MOLECULAR BIOLOGICAL PROCESSING SYSTEM" (WO/2008/080629 und PCT/EP2007/01147) beschriebenen Prozessen innerhalb einer verbesserten molekularbiologischen Prozessanlage. Als Amplifikationsprozesse können alle dem Fachmann bekannten Verfahren aber auch andere Verfahren angewendet werden, die sowohl isothermal als auch unter Anwendung bestimmter Temperaturprofϊle durchgeführt werden können. Dies sind insbesondere Whole Genome Amplification (WGA) Verfahren wie "Multiple Strand Displacement Amplification", "Linker mediated PCR", "Rolling Circle Amplification" sowie alle Arten der Polymerase-Kettenreaktion (PCR).
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE102008052084 | 2008-10-17 | ||
DE102008052084.5 | 2008-10-17 |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2010043418A2 WO2010043418A2 (de) | 2010-04-22 |
WO2010043418A3 true WO2010043418A3 (de) | 2010-07-08 |
Family
ID=41464560
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/EP2009/007477 WO2010043418A2 (de) | 2008-10-17 | 2009-10-19 | Integrierte amplifikation, prozessierung und analyse von biomolekülen in einem mikrofluidischen reaktionsträger |
Country Status (1)
Country | Link |
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WO (1) | WO2010043418A2 (de) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103890161A (zh) * | 2011-10-31 | 2014-06-25 | 株式会社日立高新技术 | 核酸扩增方法、核酸基板、核酸分析方法及核酸分析装置 |
Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2004020667A1 (en) * | 2002-09-02 | 2004-03-11 | Pamgene B.V. | Novel integrated microarray analysis |
WO2004099441A2 (en) * | 2003-05-09 | 2004-11-18 | Hyscite Discovery As | Selection and evolution of chemical libraries |
WO2006000647A1 (en) * | 2004-06-29 | 2006-01-05 | Wallac Oy | Integrated non-homogeneous nucleic acid amplification and detection |
EP1630237A2 (de) * | 2004-08-26 | 2006-03-01 | Agilent Technologies, Inc. | Isolierung von Nukleinsäurefragmenten durch Hybridisierung an eine feste Phase |
WO2007017699A2 (en) * | 2005-08-05 | 2007-02-15 | Genomica S.A.U. | In vitro diagnostic kit for identification of human papillomavirus in clinical samples |
WO2008080629A2 (de) * | 2006-12-29 | 2008-07-10 | Febit Holding Gmbh | Verbesserte molekularbiologische prozessanlage |
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2009
- 2009-10-19 WO PCT/EP2009/007477 patent/WO2010043418A2/de active Application Filing
Patent Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2004020667A1 (en) * | 2002-09-02 | 2004-03-11 | Pamgene B.V. | Novel integrated microarray analysis |
WO2004099441A2 (en) * | 2003-05-09 | 2004-11-18 | Hyscite Discovery As | Selection and evolution of chemical libraries |
WO2006000647A1 (en) * | 2004-06-29 | 2006-01-05 | Wallac Oy | Integrated non-homogeneous nucleic acid amplification and detection |
EP1630237A2 (de) * | 2004-08-26 | 2006-03-01 | Agilent Technologies, Inc. | Isolierung von Nukleinsäurefragmenten durch Hybridisierung an eine feste Phase |
WO2007017699A2 (en) * | 2005-08-05 | 2007-02-15 | Genomica S.A.U. | In vitro diagnostic kit for identification of human papillomavirus in clinical samples |
WO2008080629A2 (de) * | 2006-12-29 | 2008-07-10 | Febit Holding Gmbh | Verbesserte molekularbiologische prozessanlage |
Non-Patent Citations (2)
Title |
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AMBRETTI SIMONE ET AL: "High-throughput polymerase chain reaction chemiluminescent enzyme immunoassay for typing and quantifying human papillomavirus DNAs", 20040915, vol. 332, no. 2, 15 September 2004 (2004-09-15), pages 349 - 357, XP002421592 * |
ANONYMUS: "GeneChip TM Human Mapping 500K Array Set", INTERNET CITATION, 1 January 2006 (2006-01-01), XP002459512, Retrieved from the Internet <URL:http://www.affymetrix.com/support/technical/datasheets/500k_datasheet.pd> [retrieved on 20071122] * |
Also Published As
Publication number | Publication date |
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WO2010043418A2 (de) | 2010-04-22 |
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