WO2010038145A3 - Kit pour l’optimisation de synthèse/sécrétion de protéine - Google Patents

Kit pour l’optimisation de synthèse/sécrétion de protéine Download PDF

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Publication number
WO2010038145A3
WO2010038145A3 PCT/IB2009/007021 IB2009007021W WO2010038145A3 WO 2010038145 A3 WO2010038145 A3 WO 2010038145A3 IB 2009007021 W IB2009007021 W IB 2009007021W WO 2010038145 A3 WO2010038145 A3 WO 2010038145A3
Authority
WO
WIPO (PCT)
Prior art keywords
kit
secretion
optimisation
protein synthesis
coding sequence
Prior art date
Application number
PCT/IB2009/007021
Other languages
English (en)
Other versions
WO2010038145A2 (fr
Inventor
John E. Hesketh
Ian Fraser Pryme
Beate Stern
Hanne Ravneberg
Original Assignee
Unitargeting Research As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Unitargeting Research As filed Critical Unitargeting Research As
Priority to US13/122,378 priority Critical patent/US9018001B2/en
Priority to EP09786284.1A priority patent/EP2344524B1/fr
Publication of WO2010038145A2 publication Critical patent/WO2010038145A2/fr
Publication of WO2010038145A3 publication Critical patent/WO2010038145A3/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43509—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from crustaceans
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/48—Vector systems having a special element relevant for transcription regulating transport or export of RNA, e.g. RRE, PRE, WPRE, CTE

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Physics & Mathematics (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Insects & Arthropods (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

La présente invention concerne un kit destiné à être utilisé pour l’identification d’une cassette de sécrétion, qui, dans des cellules eucaryotes, produit un taux plus élevé de synthèse/sécrétion d’une protéine d’intérêt, dont la séquence codante est flanquée par le premier et le second ensemble d’éléments génétiques, respectivement, comparée aux autres cassettes de sécrétion présentes dans le kit contenant la séquence codante à la position correspondante, ainsi qu’un procédé dans lequel le kit est utilisé et l’utilisation dudit kit, de telle manière que la meilleure cassette de sécrétion soit obtenue.
PCT/IB2009/007021 2008-10-02 2009-10-01 Kit pour l’optimisation de synthèse/sécrétion de protéine WO2010038145A2 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
US13/122,378 US9018001B2 (en) 2008-10-02 2009-10-01 Kit for the optimisation of protein synthesis/secretion
EP09786284.1A EP2344524B1 (fr) 2008-10-02 2009-10-01 Kit pour l optimisation de synthèse/sécrétion de protéine

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US10213108P 2008-10-02 2008-10-02
SE0802078-6 2008-10-02
US61/102,131 2008-10-02
SE0802078 2008-10-02
SE0900297 2009-03-09
SE0900297-3 2009-03-09

Publications (2)

Publication Number Publication Date
WO2010038145A2 WO2010038145A2 (fr) 2010-04-08
WO2010038145A3 true WO2010038145A3 (fr) 2010-05-27

Family

ID=41800691

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB2009/007021 WO2010038145A2 (fr) 2008-10-02 2009-10-01 Kit pour l’optimisation de synthèse/sécrétion de protéine

Country Status (3)

Country Link
US (1) US9018001B2 (fr)
EP (1) EP2344524B1 (fr)
WO (1) WO2010038145A2 (fr)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20120238456A1 (en) * 2009-08-12 2012-09-20 Uni'TargetingResearch AS Rational library
JP6298039B2 (ja) * 2012-03-27 2018-03-20 キュアバック アーゲー 人工核酸分子
GB201518792D0 (en) * 2015-10-23 2015-12-09 Univ Manchester Production of proteins
KR20240001721A (ko) * 2016-09-12 2024-01-03 제네똥 산-알파 글루코시다제 변이체 및 이의 용도
IL257269A (en) * 2018-01-31 2018-04-09 Olender Tsviya Signaling is directed to the endoplasmic reticulum

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002068660A1 (fr) * 2001-02-20 2002-09-06 Aventis Pharma Deutschland Gmbh Proteine de fusion destinee a la secretion d'une proteine d'interet dans le surnageant de la culture bacterienne
WO2005001099A2 (fr) * 2003-06-25 2005-01-06 Unitargeting Research As Systeme d'expression de proteine

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002068660A1 (fr) * 2001-02-20 2002-09-06 Aventis Pharma Deutschland Gmbh Proteine de fusion destinee a la secretion d'une proteine d'interet dans le surnageant de la culture bacterienne
WO2005001099A2 (fr) * 2003-06-25 2005-01-06 Unitargeting Research As Systeme d'expression de proteine

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
J-L VEYRUNE ET AL: "A localisation signal in the 3' untranslated region of c-mac mRNA targets c-myc mRNA and beta-globin reporter sequences to the prinuclear cytoplasm and cytoskeletal-bound polysomes", JOURNAL OF CELL SCIENCE, CAMBRIDGE UNIVERSITY PRESS, LONDON, GB, vol. 109, 1 January 1996 (1996-01-01), pages 1185 - 1194, XP002086116, ISSN: 0021-9533 *
KRISTINE A PARTRIDGE ET AL: "Competition between the signal sequence and a 3'UTR localisation signal during redirection of beta-globin mRNA to the endoplasmic reticulum: implications for biotechnology", CYTOTECHNOLOGY, KLUWER ACADEMIC PUBLISHERS, DO, vol. 30, no. 1-3, 1 May 1999 (1999-05-01), pages 37 - 47, XP019236605, ISSN: 1573-0778 *

Also Published As

Publication number Publication date
EP2344524B1 (fr) 2019-12-04
US9018001B2 (en) 2015-04-28
EP2344524A2 (fr) 2011-07-20
US20110262905A1 (en) 2011-10-27
WO2010038145A2 (fr) 2010-04-08

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