WO2010034428A3 - Verfahren zur reduzierung von ablagerungen bei der kultivierung von organismen - Google Patents

Verfahren zur reduzierung von ablagerungen bei der kultivierung von organismen Download PDF

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Publication number
WO2010034428A3
WO2010034428A3 PCT/EP2009/006722 EP2009006722W WO2010034428A3 WO 2010034428 A3 WO2010034428 A3 WO 2010034428A3 EP 2009006722 W EP2009006722 W EP 2009006722W WO 2010034428 A3 WO2010034428 A3 WO 2010034428A3
Authority
WO
WIPO (PCT)
Prior art keywords
organisms
cultivation
deposits during
reducing deposits
adhere
Prior art date
Application number
PCT/EP2009/006722
Other languages
English (en)
French (fr)
Other versions
WO2010034428A2 (de
Inventor
Helmut Brod
Björn FRAHM
Joerg Kauling
Original Assignee
Bayer Technology Services Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bayer Technology Services Gmbh filed Critical Bayer Technology Services Gmbh
Priority to CN2009801377765A priority Critical patent/CN102272285A/zh
Priority to EP09778578A priority patent/EP2342315A2/de
Priority to US13/062,556 priority patent/US20110165677A1/en
Publication of WO2010034428A2 publication Critical patent/WO2010034428A2/de
Publication of WO2010034428A3 publication Critical patent/WO2010034428A3/de

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F23/00—Mixing according to the phases to be mixed, e.g. dispersing or emulsifying
    • B01F23/20—Mixing gases with liquids
    • B01F23/23—Mixing gases with liquids by introducing gases into liquid media, e.g. for producing aerated liquids
    • B01F23/231—Mixing gases with liquids by introducing gases into liquid media, e.g. for producing aerated liquids by bubbling
    • B01F23/23105—Arrangement or manipulation of the gas bubbling devices
    • B01F23/2312—Diffusers
    • B01F23/23124—Diffusers consisting of flexible porous or perforated material, e.g. fabric
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F23/00—Mixing according to the phases to be mixed, e.g. dispersing or emulsifying
    • B01F23/20—Mixing gases with liquids
    • B01F23/23—Mixing gases with liquids by introducing gases into liquid media, e.g. for producing aerated liquids
    • B01F23/231—Mixing gases with liquids by introducing gases into liquid media, e.g. for producing aerated liquids by bubbling
    • B01F23/23105—Arrangement or manipulation of the gas bubbling devices
    • B01F23/2312—Diffusers
    • B01F23/23124—Diffusers consisting of flexible porous or perforated material, e.g. fabric
    • B01F23/231244—Dissolving, hollow fiber membranes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F23/00—Mixing according to the phases to be mixed, e.g. dispersing or emulsifying
    • B01F23/20—Mixing gases with liquids
    • B01F23/23—Mixing gases with liquids by introducing gases into liquid media, e.g. for producing aerated liquids
    • B01F23/231—Mixing gases with liquids by introducing gases into liquid media, e.g. for producing aerated liquids by bubbling
    • B01F23/23105—Arrangement or manipulation of the gas bubbling devices
    • B01F23/2312—Diffusers
    • B01F23/23126—Diffusers characterised by the shape of the diffuser element
    • B01F23/231265—Diffusers characterised by the shape of the diffuser element being tubes, tubular elements, cylindrical elements or set of tubes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F31/00—Mixers with shaking, oscillating, or vibrating mechanisms
    • B01F31/44—Mixers with shaking, oscillating, or vibrating mechanisms with stirrers performing an oscillatory, vibratory or shaking movement
    • B01F31/445—Mixers with shaking, oscillating, or vibrating mechanisms with stirrers performing an oscillatory, vibratory or shaking movement performing an oscillatory movement about an axis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/24—Gas permeable parts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/02—Stirrer or mobile mixing elements
    • C12M27/04—Stirrer or mobile mixing elements with introduction of gas through the stirrer or mixing element
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M39/00—Means for cleaning the apparatus or avoiding unwanted deposits of microorganisms

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Clinical Laboratory Science (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Die Erfindung betrifft ein Verfahren zur Reduzierung von Ablagerungen bei der Kultivierung von Organismen, insbesondere von Zellkulturen, welche zur Agglomeration bzw. Anhaftung an den Bioreaktor und seine Elemente neigen, oder bei denen Zelldebris oder Substanzen leicht agglomerieren bzw. anhaften.
PCT/EP2009/006722 2008-09-26 2009-09-17 Verfahren zur reduzierung von ablagerungen bei der kultivierung von organismen WO2010034428A2 (de)

Priority Applications (3)

Application Number Priority Date Filing Date Title
CN2009801377765A CN102272285A (zh) 2008-09-26 2009-09-17 在生物体培养期间减少沉积数量的方法
EP09778578A EP2342315A2 (de) 2008-09-26 2009-09-17 Verfahren zur reduzierung von ablagerungen bei der kultivierung von organismen
US13/062,556 US20110165677A1 (en) 2008-09-26 2009-09-17 Method for reducing deposits during the cultivation of organisms

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102008049120A DE102008049120A1 (de) 2008-09-26 2008-09-26 Verfahren zur Reduzierung von Ablagerungen bei der Kultivierung von Organismen
DE102008049120.9 2008-09-26

Publications (2)

Publication Number Publication Date
WO2010034428A2 WO2010034428A2 (de) 2010-04-01
WO2010034428A3 true WO2010034428A3 (de) 2010-07-08

Family

ID=41719800

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2009/006722 WO2010034428A2 (de) 2008-09-26 2009-09-17 Verfahren zur reduzierung von ablagerungen bei der kultivierung von organismen

Country Status (5)

Country Link
US (1) US20110165677A1 (de)
EP (1) EP2342315A2 (de)
CN (1) CN102272285A (de)
DE (1) DE102008049120A1 (de)
WO (1) WO2010034428A2 (de)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20120282677A1 (en) 2011-05-03 2012-11-08 Bayer Intellectual Property Gmbh Photobioreactor comprising rotationally oscillating light sources
EP2707387B1 (de) 2011-05-13 2019-12-25 Octapharma AG Verfahren zur erhöhung der produktivität von eukaryotischen zellen bei der herstellung von rekombinantem fviii
US9884295B2 (en) 2012-10-08 2018-02-06 Doosan Heavy Industries & Construction Co., Ltd. Membrane bioreactor system using reciprocating membrane
CN104403937B (zh) * 2014-12-17 2016-08-17 大连大学 旋转内循环气升式膜生物反应器及其工艺系统

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0172478A1 (de) * 1984-08-03 1986-02-26 Gesellschaft für Biotechnologische Forschung mbH (GBF) Verfahren und Vorrichtung zur blasenfreien Begasung von Flüssigkeiten, insbesondere von Kulturmedien zur Vermehrung von Gewebekulturen
DE9215153U1 (de) * 1992-11-06 1993-01-07 B. Braun Biotech International Gmbh, 3508 Melsungen, De
WO2007098850A1 (de) * 2006-02-24 2007-09-07 Bayer Technology Services Gmbh Verfahren und vorrichtung zur be- und entgasung von flüssigkeiten, insbesondere in der biotechnologie und speziell von zellkulturen

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8515636D0 (en) 1985-06-20 1985-07-24 Celltech Ltd Fermenter
DE3535183A1 (de) 1985-10-02 1987-04-16 Biotechnolog Forschung Gmbh Vorrichtung und verfahren zur blasenfreien begasung von fluessigkeiten, insbesondere von kulturmedien zur vermehrung von gewebekulturen
GB8607046D0 (en) 1986-03-21 1986-04-30 Fisons Plc Culture device
US4960706A (en) 1989-03-27 1990-10-02 Baxter International, Inc. Static oxygenator for suspension culture of animal cells
PT1451290E (pt) 2001-08-31 2011-03-09 Bayer Schering Pharma Ag Uma unidade e um processo para levar a cabo a fermentação com elevada densidade celular
CN1155690C (zh) * 2002-07-04 2004-06-30 中国人民解放军第三军医大学第一附属医院 循环和生理应力模拟工程化组织三维培养装置
CN1249217C (zh) * 2003-01-27 2006-04-05 英科新创(厦门)科技有限公司 细胞培养方法和装置
US7122121B1 (en) * 2004-05-28 2006-10-17 Jiang Ji Advanced submerged membrane modules, systems and processes
DE102005053334A1 (de) 2005-11-07 2007-05-24 Bayer Technology Services Gmbh Module zur Membranbegasung
DE102006062634A1 (de) 2006-12-23 2008-06-26 Stiftung Alfred-Wegener-Institut für Polar- und Meeresforschung Stiftung des öffentlichen Rechts Kulturvorrichtung für aquatische Organismen

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0172478A1 (de) * 1984-08-03 1986-02-26 Gesellschaft für Biotechnologische Forschung mbH (GBF) Verfahren und Vorrichtung zur blasenfreien Begasung von Flüssigkeiten, insbesondere von Kulturmedien zur Vermehrung von Gewebekulturen
DE9215153U1 (de) * 1992-11-06 1993-01-07 B. Braun Biotech International Gmbh, 3508 Melsungen, De
WO2007098850A1 (de) * 2006-02-24 2007-09-07 Bayer Technology Services Gmbh Verfahren und vorrichtung zur be- und entgasung von flüssigkeiten, insbesondere in der biotechnologie und speziell von zellkulturen

Also Published As

Publication number Publication date
CN102272285A (zh) 2011-12-07
DE102008049120A1 (de) 2010-04-01
US20110165677A1 (en) 2011-07-07
WO2010034428A2 (de) 2010-04-01
EP2342315A2 (de) 2011-07-13

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