WO2010019813A3 - Production of fatty actds by genetically modified photosynthetic organisms - Google Patents

Production of fatty actds by genetically modified photosynthetic organisms Download PDF

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Publication number
WO2010019813A3
WO2010019813A3 PCT/US2009/053772 US2009053772W WO2010019813A3 WO 2010019813 A3 WO2010019813 A3 WO 2010019813A3 US 2009053772 W US2009053772 W US 2009053772W WO 2010019813 A3 WO2010019813 A3 WO 2010019813A3
Authority
WO
WIPO (PCT)
Prior art keywords
production
photosynthetic
compositions
fatty
genetically modified
Prior art date
Application number
PCT/US2009/053772
Other languages
French (fr)
Other versions
WO2010019813A2 (en
Inventor
Michael Mendez
Bryan O'neill
Michael Burkart
Craig Behnke
Soyan Lieberman
Vince Bielinski
Yan Poon
Original Assignee
Sapphire Energy, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sapphire Energy, Inc. filed Critical Sapphire Energy, Inc.
Publication of WO2010019813A2 publication Critical patent/WO2010019813A2/en
Publication of WO2010019813A3 publication Critical patent/WO2010019813A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0083—Miscellaneous (1.14.99)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/24—Preparation of oxygen-containing organic compounds containing a carbonyl group
    • C12P7/26—Ketones
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6463—Glycerides obtained from glyceride producing microorganisms, e.g. single cell oil

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Cell Biology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The present invention relates to compositions that facilitate the production of enzymes by genetically modified photosynthetic microorganisms wherein the enzymes contribute to the synthesis of fatty acids, lipids or oils. In some embodiments the compositions are one or more vectors that encode one or more polypeptides that increase production of fatty acids, lipids or oils. The vectors may comprise promoters and other nucleic acid sequences configured for expression of the polypeptides in the chloroplast of non-vascular, photosynthetic organisms. In some embodiments the compositions are genetically altered photosynthetic microorgansims or genetically altered chloroplasts. The present invention provides methods of producing one or more proteins, including fatty acid synthesizing enzymes in a photosynthetic microorganism. In some embodiments the present invention is a method of preparing a fuel. The methods disclosed herein allow for the production of crude or refined biofuels, including ethanol.
PCT/US2009/053772 2008-08-13 2009-08-13 Production of fatty actds by genetically modified photosynthetic organisms WO2010019813A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US8859108P 2008-08-13 2008-08-13
US61/088,591 2008-08-13

Publications (2)

Publication Number Publication Date
WO2010019813A2 WO2010019813A2 (en) 2010-02-18
WO2010019813A3 true WO2010019813A3 (en) 2010-07-22

Family

ID=41669694

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2009/053772 WO2010019813A2 (en) 2008-08-13 2009-08-13 Production of fatty actds by genetically modified photosynthetic organisms

Country Status (1)

Country Link
WO (1) WO2010019813A2 (en)

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SG182157A1 (en) 2007-06-01 2012-07-30 Solazyme Inc Production of oil in microorganisms
US8048654B2 (en) 2010-06-09 2011-11-01 Joule Unlimited Technologies, Inc. Methods and compositions for the recombinant biosynthesis of fatty acids and esters
EP2265724A4 (en) 2008-04-09 2013-01-23 Solazyme Inc Direct chemical modification of microbial biomass and microbial oils
CN102712858B (en) 2008-11-28 2015-08-12 索拉兹米公司 Tailor-made oil is prepared in restructuring heterotroph microorganism
JP5670346B2 (en) 2008-12-23 2015-02-18 マトリックス ジェネティクス， エルエルシー Glycogen-reduced, modified photosynthetic microorganisms and their use in producing carbon-based products
WO2011127069A1 (en) 2010-04-06 2011-10-13 Targeted Growth, Inc. Modified photosynthetic microorganisms for producing lipids
CN103124499B (en) 2010-05-28 2016-09-28 泰拉瑞亚控股公司 Comprise the food compositions of tailor-made oil
MX354145B (en) 2010-11-03 2018-02-14 Terravia Holdings Inc Microbial oils with lowered pour points, dielectric fluids produced therefrom, and related methods.
WO2012087673A1 (en) 2010-12-23 2012-06-28 Exxonmobil Research And Engineering Company Prokaryotic acyl-acp thioesterases for producing fatty acids in genetically engineered microorganisms
BR112013016020A2 (en) 2010-12-23 2017-07-11 Exxonmobil Res & Eng Co genetically engineered microorganisms comprising 4-hydroxybenzoyl co-thioesterases and methods for using them for the production of free fatty acids and fatty acid derivatives
KR102117225B1 (en) 2011-02-02 2020-06-02 테라비아 홀딩스 인코포레이티드 Tailored oils produced from recombinant oleaginous microorganisms
AU2012253803A1 (en) 2011-05-06 2013-12-05 Terravia Holdings, Inc. Genetically engineered microorganisms that metabolize xylose
WO2013130406A1 (en) * 2012-02-24 2013-09-06 Sapphire Energy, Inc. Lipid and growth trait genes
CA2870364A1 (en) 2012-04-18 2013-10-24 Solazyme, Inc. Recombinant microbes with modified fatty acid synthetic pathway enzymes and uses thereof
US9719114B2 (en) 2012-04-18 2017-08-01 Terravia Holdings, Inc. Tailored oils
US10098371B2 (en) 2013-01-28 2018-10-16 Solazyme Roquette Nutritionals, LLC Microalgal flour
US9567615B2 (en) 2013-01-29 2017-02-14 Terravia Holdings, Inc. Variant thioesterases and methods of use
US9816079B2 (en) 2013-01-29 2017-11-14 Terravia Holdings, Inc. Variant thioesterases and methods of use
US9783836B2 (en) 2013-03-15 2017-10-10 Terravia Holdings, Inc. Thioesterases and cells for production of tailored oils
US9290749B2 (en) 2013-03-15 2016-03-22 Solazyme, Inc. Thioesterases and cells for production of tailored oils
US9249252B2 (en) 2013-04-26 2016-02-02 Solazyme, Inc. Low polyunsaturated fatty acid oils and uses thereof
FR3009619B1 (en) 2013-08-07 2017-12-29 Roquette Freres BIOMASS COMPOSITIONS OF MICROALGUES RICH IN PROTEINS OF SENSORY QUALITY OPTIMIZED
SG10201802834YA (en) 2013-10-04 2018-05-30 Terravia Holdings Inc Tailored oils
US9394550B2 (en) 2014-03-28 2016-07-19 Terravia Holdings, Inc. Lauric ester compositions
EP3167053B1 (en) 2014-07-10 2019-10-09 Corbion Biotech, Inc. Novel ketoacyl acp synthase genes and uses thereof
BR112017001404A2 (en) 2014-07-24 2017-11-21 Terravia Holdings Inc Variant thioesterases and methods of use
CN107208103A (en) 2014-09-18 2017-09-26 泰拉瑞亚控股公司 Acyl group ACP thioesterases and its mutant

Citations (3)

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WO2004000871A2 (en) * 2002-06-21 2003-12-31 Monsanto Technology Llc Thioesterase-related nucleic acid sequences and methods
EP1777296A2 (en) * 2005-10-14 2007-04-25 Metanomics GmbH Process for the production of fine chemicals
WO2007106905A2 (en) * 2006-03-15 2007-09-20 Martek Biosciences Corporation Polyunsaturated fatty acid production in heterologous organisms using pufa polyketide synthase systems

Patent Citations (3)

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Publication number Priority date Publication date Assignee Title
WO2004000871A2 (en) * 2002-06-21 2003-12-31 Monsanto Technology Llc Thioesterase-related nucleic acid sequences and methods
EP1777296A2 (en) * 2005-10-14 2007-04-25 Metanomics GmbH Process for the production of fine chemicals
WO2007106905A2 (en) * 2006-03-15 2007-09-20 Martek Biosciences Corporation Polyunsaturated fatty acid production in heterologous organisms using pufa polyketide synthase systems

Non-Patent Citations (4)

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DATABASE GENBANK 21 November 2007 (2007-11-21), Database accession no. EDP08596 *
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PNAS, vol. 104, no. 11, 2007, pages 4742 - 4747 *

Also Published As

Publication number Publication date
WO2010019813A2 (en) 2010-02-18

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