WO2010017518A3 - Connecting microrna genes to the core transcriptional regulatory circuitry of embryonic stem cells - Google Patents

Connecting microrna genes to the core transcriptional regulatory circuitry of embryonic stem cells Download PDF

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Publication number
WO2010017518A3
WO2010017518A3 PCT/US2009/053214 US2009053214W WO2010017518A3 WO 2010017518 A3 WO2010017518 A3 WO 2010017518A3 US 2009053214 W US2009053214 W US 2009053214W WO 2010017518 A3 WO2010017518 A3 WO 2010017518A3
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WO
WIPO (PCT)
Prior art keywords
stem cells
embryonic stem
transcriptional regulatory
microrna genes
regulatory circuitry
Prior art date
Application number
PCT/US2009/053214
Other languages
French (fr)
Other versions
WO2010017518A2 (en
Inventor
Rudolph Jaenisch
Richard A. Young
Alexander Marson
Stuart Levine
Original Assignee
Whitehead Institute For Biomedical Research
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Whitehead Institute For Biomedical Research filed Critical Whitehead Institute For Biomedical Research
Publication of WO2010017518A2 publication Critical patent/WO2010017518A2/en
Publication of WO2010017518A3 publication Critical patent/WO2010017518A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1089—Design, preparation, screening or analysis of libraries using computer algorithms
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B20/00—ICT specially adapted for functional genomics or proteomics, e.g. genotype-phenotype associations
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B20/00—ICT specially adapted for functional genomics or proteomics, e.g. genotype-phenotype associations
    • G16B20/20—Allele or variant detection, e.g. single nucleotide polymorphism [SNP] detection
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B20/00—ICT specially adapted for functional genomics or proteomics, e.g. genotype-phenotype associations
    • G16B20/30—Detection of binding sites or motifs

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Evolutionary Biology (AREA)
  • Medical Informatics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Theoretical Computer Science (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biochemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present invention provides, among other things, promoters for mouse and human microRNA genes and methods of use thereof.
PCT/US2009/053214 2008-08-07 2009-08-07 Connecting microrna genes to the core transcriptional regulatory circuitry of embryonic stem cells WO2010017518A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US18821108P 2008-08-07 2008-08-07
US61/188,211 2008-08-07

Publications (2)

Publication Number Publication Date
WO2010017518A2 WO2010017518A2 (en) 2010-02-11
WO2010017518A3 true WO2010017518A3 (en) 2010-06-03

Family

ID=41664224

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2009/053214 WO2010017518A2 (en) 2008-08-07 2009-08-07 Connecting microrna genes to the core transcriptional regulatory circuitry of embryonic stem cells

Country Status (1)

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WO (1) WO2010017518A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103484544B (en) * 2013-09-17 2015-11-25 遵义医学院 A kind of method of Microrna promoter function analysis
CN107358062B (en) * 2017-06-02 2020-05-22 西安电子科技大学 Construction method of double-layer gene regulation and control network

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007030678A2 (en) * 2005-09-07 2007-03-15 Whitehead Institute For Biomedical Research Methods of genome-wide location analysis in stem cells

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007030678A2 (en) * 2005-09-07 2007-03-15 Whitehead Institute For Biomedical Research Methods of genome-wide location analysis in stem cells

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
BOYER ET AL.: "Core Transcriptional Regulatory Circuitry in Human Embryonic Stem Cells.", CELL., vol. 122, 23 September 2005 (2005-09-23), pages 947 - 956 *
MARSON, ALEXANDER., PROGRAMMING AND REPROGRAMMING CELLULAR IDENTITY [THESIS]., 29 May 2008 (2008-05-29), Retrieved from the Internet <URL:http://dspace.mit.edu/bitstream/handle/1721.1/43223/259233539.pdf?sequence=1> [retrieved on 20100106] *

Also Published As

Publication number Publication date
WO2010017518A2 (en) 2010-02-11

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