WO2010013947A3 - Growth method for natural killer cells - Google Patents

Growth method for natural killer cells Download PDF

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Publication number
WO2010013947A3
WO2010013947A3 PCT/KR2009/004228 KR2009004228W WO2010013947A3 WO 2010013947 A3 WO2010013947 A3 WO 2010013947A3 KR 2009004228 W KR2009004228 W KR 2009004228W WO 2010013947 A3 WO2010013947 A3 WO 2010013947A3
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WO
WIPO (PCT)
Prior art keywords
natural killer
killer cells
growth method
present
cells
Prior art date
Application number
PCT/KR2009/004228
Other languages
French (fr)
Korean (ko)
Other versions
WO2010013947A2 (en
Inventor
안용운
정미영
허대석
황유경
Original Assignee
주식회사 녹십자
서울대학교병원
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 주식회사 녹십자, 서울대학교병원 filed Critical 주식회사 녹십자
Priority to JP2011521023A priority Critical patent/JP5358683B2/en
Priority to CN200980130121.5A priority patent/CN102112600B/en
Publication of WO2010013947A2 publication Critical patent/WO2010013947A2/en
Publication of WO2010013947A3 publication Critical patent/WO2010013947A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0646—Natural killers cells [NK], NKT cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/02—Preparation of hybrid cells by fusion of two or more cells, e.g. protoplast fusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/23—Interleukins [IL]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/50—Cell markers; Cell surface determinants
    • C12N2501/515—CD3, T-cell receptor complex

Abstract

The present invention relates to an improved growth method for natural killer cells (NK cells). More specifically, the present invention relates to a growth method for natural killer cells, which comprises a step of culturing natural killer cells in a medium containing anti-CD3 antibodies and interleukin proteins in the presence of peripheral blood leukocytes. The present invention provides an innovative growth method for natural killer cells, which can obtain a large amount of natural killer cells by remarkably increasing the growth rate compared with conventional growth methods for natural killer cells.
PCT/KR2009/004228 2008-07-29 2009-07-29 Growth method for natural killer cells WO2010013947A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
JP2011521023A JP5358683B2 (en) 2008-07-29 2009-07-29 Method of growing natural killer cells
CN200980130121.5A CN102112600B (en) 2008-07-29 2009-07-29 Growth method for natural killer cells

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR10-2008-0074069 2008-07-29
KR1020080074069A KR101133185B1 (en) 2008-07-29 2008-07-29 Method for Proliferating Natural Killer cell

Publications (2)

Publication Number Publication Date
WO2010013947A2 WO2010013947A2 (en) 2010-02-04
WO2010013947A3 true WO2010013947A3 (en) 2010-05-27

Family

ID=41610847

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2009/004228 WO2010013947A2 (en) 2008-07-29 2009-07-29 Growth method for natural killer cells

Country Status (4)

Country Link
JP (1) JP5358683B2 (en)
KR (1) KR101133185B1 (en)
CN (1) CN102112600B (en)
WO (1) WO2010013947A2 (en)

Cited By (1)

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CN103620022B (en) * 2011-06-24 2015-09-30 国立大学法人九州大学 The amplification method of NK cell

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NZ599825A (en) 2007-09-28 2014-10-31 Anthrogenesis Corp Tumor suppression using human placental perfusate and human placenta-derived intermediate natural killer cells
CN107760648A (en) 2010-07-13 2018-03-06 人类起源公司 Produce the method for NK, thus obtained cell colony and application thereof
KR101039843B1 (en) * 2010-08-30 2011-06-09 주식회사 엔케이바이오 A medium composition for cultivating self activated lymphocyte and the cultivation method using the same
KR101121101B1 (en) * 2010-09-08 2012-03-20 고려대학교 산학협력단 A composition for promoting nk cell proliferation comprising clusterin
TWI439275B (en) * 2010-12-31 2014-06-01 Glory Biomedical Co Ltd An ex vivo method for expanding human natural killer cells
JPWO2012099093A1 (en) * 2011-01-21 2014-06-30 株式会社日本バイオセラピー研究所 Method for producing NK cell-enhanced blood product
JP5840876B2 (en) * 2011-06-24 2016-01-06 テラ株式会社 Compositions and methods for amplifying NK cells
WO2013094988A1 (en) * 2011-12-22 2013-06-27 Mogam Biotechnology Research Institute Method for producing natural killer cells, natural killer cells produced thereby, and composition for treating cancers and infectious diseases containing the same
CN102839153A (en) * 2012-09-13 2012-12-26 济南泰生生物技术有限公司 Amplifying, freezing and storing and recovering method of activated lymphocyte with CD3+CD8+as major
KR101923848B1 (en) 2013-01-15 2018-11-29 히로유키 아베 Method for manufacturing immunocyte-containing composition, and cancer-treating composition
CN115282165A (en) 2013-02-05 2022-11-04 细胞结构公司 Natural killer cells from placenta
JP6588465B2 (en) * 2014-01-03 2019-10-09 アカデミア シニカＡｃａｄｅｍｉａ Ｓｉｎｉｃａ Modified natural killer cells, compositions and uses thereof
CN103923879B (en) * 2014-04-29 2016-03-30 湖北华赛生物医药技术有限公司 A kind of preparation method of NK cytokine mixture and application thereof
KR101697473B1 (en) 2014-11-26 2017-01-18 주식회사 녹십자랩셀 Method for Preparation of Natural Killer Cells Using T Cells
CN104593324B (en) * 2014-11-28 2018-04-13 广州赛莱拉干细胞科技股份有限公司 A kind of culture medium of natural killer cells and the amplification cultivation method of natural killer cells
WO2016122014A1 (en) * 2015-01-27 2016-08-04 한국생명공학연구원 Method for mass-producing natural killer cells and use of natural killer cells obtained by the method as anticancer agent
KR101909879B1 (en) * 2015-06-24 2018-10-19 주식회사 차바이오텍 Method and Composition for Proliferating of Natural Killer Cell
WO2016209021A1 (en) * 2015-06-24 2016-12-29 주식회사 차바이오텍 Method for proliferating natural killer cells and composition for proliferating natural killer cells
CN105441390A (en) * 2015-11-18 2016-03-30 深圳爱生再生医学科技有限公司 In-vitro three-dimensional amplification culture method for NK cells
CN106148282B (en) * 2016-07-29 2020-04-03 北京时合生物科技有限公司 Natural killer cell culture method
CN106434554B (en) * 2016-08-31 2019-07-26 北京同立海源生物科技有限公司 The preparation method of NK cell
US10300089B2 (en) * 2016-11-08 2019-05-28 University Of Central Florida Research Foundation, Inc. Methods for high scale therapeutic production of memory NK cells
KR101969045B1 (en) * 2016-11-22 2019-04-19 신동혁 Culture media kits for NK cell Cultivation, NK cell culture method using the same, NK Cell media solution without blood serum, and cosmetic composition comprising the serum free conditioned media
CN107541491A (en) * 2017-10-12 2018-01-05 广州金晟生物科技有限公司 A kind of NK cell injuring models method
CN108004210B (en) * 2017-12-18 2020-09-04 吉林省吉恩致合生物治疗技术有限公司 Method for inducing and amplifying NK cells with ADCC effect in large quantity
IL276365B2 (en) 2018-02-01 2023-10-01 Nkmax Co Ltd Method of producing natural killer cells and composition for treating cancer
WO2019182392A1 (en) * 2018-03-23 2019-09-26 주식회사 녹십자랩셀 Method for producing natural killer cells
KR102232321B1 (en) * 2018-03-23 2021-03-26 주식회사 녹십자랩셀 Method for Preparing NK Cell
EP3884041A2 (en) * 2018-11-21 2021-09-29 Indapta Therapeutics, Inc. Methods for expansion of natural killer (nk) cell subset and related compositions and methods
CN109810944A (en) * 2019-01-24 2019-05-28 安徽瑞达健康产业有限公司 A kind of stabilization in vitro amplification high-purity, the method for high cytotoxic activity NK cell
KR102137954B1 (en) * 2019-05-16 2020-07-27 (주)녹십자셀 Activated lymphocyte including cytokine induced killer cell and preparing method thereof
CN113832101A (en) * 2021-09-03 2021-12-24 秦红 Preparation method for efficient in-vitro amplification of natural killer cells

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Also Published As

Publication number Publication date
JP2011529341A (en) 2011-12-08
KR101133185B1 (en) 2012-04-06
WO2010013947A2 (en) 2010-02-04
CN102112600B (en) 2014-09-17
JP5358683B2 (en) 2013-12-04
CN102112600A (en) 2011-06-29
KR20100012586A (en) 2010-02-08

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