WO2010003759A3 - Cell culturing method - Google Patents

Cell culturing method Download PDF

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Publication number
WO2010003759A3
WO2010003759A3 PCT/EP2009/057281 EP2009057281W WO2010003759A3 WO 2010003759 A3 WO2010003759 A3 WO 2010003759A3 EP 2009057281 W EP2009057281 W EP 2009057281W WO 2010003759 A3 WO2010003759 A3 WO 2010003759A3
Authority
WO
WIPO (PCT)
Prior art keywords
cell culturing
culturing method
cells
bioreactor
growing
Prior art date
Application number
PCT/EP2009/057281
Other languages
French (fr)
Other versions
WO2010003759A2 (en
Inventor
Gerben Meile Zijlstra
Original Assignee
Dsm Ip Assets B.V.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dsm Ip Assets B.V. filed Critical Dsm Ip Assets B.V.
Publication of WO2010003759A2 publication Critical patent/WO2010003759A2/en
Publication of WO2010003759A3 publication Critical patent/WO2010003759A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/16—Hollow fibers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/12—Pulsatile flow
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation

Landscapes

  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The present invention relates to the use of a retention device with tangential flow for the improvement of mass transfer in a bioreactor. The method according to this invention is particularly suited for growing of cells and maintaining cells at high cell density.
PCT/EP2009/057281 2008-06-17 2009-06-12 Cell culturing method WO2010003759A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP08158442 2008-06-17
EP08158442.7 2008-06-17

Publications (2)

Publication Number Publication Date
WO2010003759A2 WO2010003759A2 (en) 2010-01-14
WO2010003759A3 true WO2010003759A3 (en) 2010-03-04

Family

ID=40030361

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2009/057281 WO2010003759A2 (en) 2008-06-17 2009-06-12 Cell culturing method

Country Status (1)

Country Link
WO (1) WO2010003759A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107190034A (en) * 2010-10-05 2017-09-22 诺沃—诺迪斯克保健股份有限公司 Produce method of protein
US20190169559A1 (en) 2016-04-15 2019-06-06 Boehringer Ingelheim International Gmbh Cell retention device and method

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0224734A1 (en) * 1985-11-04 1987-06-10 Endotronics Inc. Apparatus and method for culturing cells, removing waste and concentrating product
US20030054544A1 (en) * 2001-09-14 2003-03-20 Medcell Biologics, Inc. Oxygen enriched bioreactor and method of culturing cells
US20050019914A1 (en) * 2003-07-24 2005-01-27 Aventis Pharma Deutschland Gmbh Perfusion process for producing erythropoietin
WO2005042768A2 (en) * 2003-11-03 2005-05-12 Centocor, Inc. Method for maintaining low shear in a bioprocessing system
WO2005095578A1 (en) * 2004-03-05 2005-10-13 Dsm Ip Assets B.V. Process for cell culturing by continuous perfusion and alternating tangential flow

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0224734A1 (en) * 1985-11-04 1987-06-10 Endotronics Inc. Apparatus and method for culturing cells, removing waste and concentrating product
US20030054544A1 (en) * 2001-09-14 2003-03-20 Medcell Biologics, Inc. Oxygen enriched bioreactor and method of culturing cells
US20050019914A1 (en) * 2003-07-24 2005-01-27 Aventis Pharma Deutschland Gmbh Perfusion process for producing erythropoietin
WO2005042768A2 (en) * 2003-11-03 2005-05-12 Centocor, Inc. Method for maintaining low shear in a bioprocessing system
WO2005095578A1 (en) * 2004-03-05 2005-10-13 Dsm Ip Assets B.V. Process for cell culturing by continuous perfusion and alternating tangential flow

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
CASTILHO L R ET AL: "CELL RETENTION DEVICES FOR SUSPENDED-CELL PERFUSION CULTURES", 1 January 2002, ADVANCES IN BIOCHEMICAL ENGINEERING, BIOTECHNOLOGY, SPRINGER, BERLIN, DE, PAGE(S) 129 - 169, ISSN: 0724-6145, XP009038515 *
WOODSIDE S M ET AL: "Mammalian cell retention devices for stirred perfusion bioreactors", 1 January 1998, CYTOTECHNOLOGY, KLUWER ACADEMIC PUBLISHERS, DORDRECHT, NL, PAGE(S) 163 - 175, ISSN: 0920-9069, XP002301951 *

Also Published As

Publication number Publication date
WO2010003759A2 (en) 2010-01-14

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