WO2009154378A3 - Method for preparing oxides using germs in corynebacterium - Google Patents

Method for preparing oxides using germs in corynebacterium Download PDF

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Publication number
WO2009154378A3
WO2009154378A3 PCT/KR2009/003181 KR2009003181W WO2009154378A3 WO 2009154378 A3 WO2009154378 A3 WO 2009154378A3 KR 2009003181 W KR2009003181 W KR 2009003181W WO 2009154378 A3 WO2009154378 A3 WO 2009154378A3
Authority
WO
WIPO (PCT)
Prior art keywords
corynebacterium
preparing
oxides
germs
substrate
Prior art date
Application number
PCT/KR2009/003181
Other languages
French (fr)
Korean (ko)
Other versions
WO2009154378A2 (en
Inventor
박진병
두은희
서진호
Original Assignee
이화여자대학교 산학협력단
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 이화여자대학교 산학협력단 filed Critical 이화여자대학교 산학협력단
Publication of WO2009154378A2 publication Critical patent/WO2009154378A2/en
Publication of WO2009154378A3 publication Critical patent/WO2009154378A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/02—Oxygen as only ring hetero atoms
    • C12P17/06—Oxygen as only ring hetero atoms containing a six-membered hetero ring, e.g. fluorescein
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/77—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Corynebacterium; for Brevibacterium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0073—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14) with NADH or NADPH as one donor, and incorporation of one atom of oxygen 1.14.13
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C12Y114/13—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with NADH or NADPH as one donor, and incorporation of one atom of oxygen (1.14.13)
    • C12Y114/13022—Cyclohexanone monooxygenase (1.14.13.22)

Abstract

The present invention relates to a method for preparing oxides characterized in that strains in corynebacterium transformed with genes that code an oxygenase capable of adding oxygen to a substrate are employed in a method for preparing an oxide by inducing an oxidation wherein oxygen is added to a substrate, thus remarkably increasing the yield rate of oxides as compared to conventional methods which use existing coliform bacilli.
PCT/KR2009/003181 2008-06-17 2009-06-15 Method for preparing oxides using germs in corynebacterium WO2009154378A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1020080056856A KR20090131073A (en) 2008-06-17 2008-06-17 Method for the production of oxygen compounds using corynebacterium sp
KR10-2008-0056856 2008-06-17

Publications (2)

Publication Number Publication Date
WO2009154378A2 WO2009154378A2 (en) 2009-12-23
WO2009154378A3 true WO2009154378A3 (en) 2010-04-01

Family

ID=41434539

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2009/003181 WO2009154378A2 (en) 2008-06-17 2009-06-15 Method for preparing oxides using germs in corynebacterium

Country Status (2)

Country Link
KR (1) KR20090131073A (en)
WO (1) WO2009154378A2 (en)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6365376B1 (en) * 1999-02-19 2002-04-02 E. I. Du Pont De Nemours And Company Genes and enzymes for the production of adipic acid intermediates
WO2007101867A1 (en) * 2006-03-09 2007-09-13 Basf Se PROCESS FOR THE PRODUCTION OF β-LYSINE
WO2008013966A2 (en) * 2006-07-28 2008-01-31 Johns Hopkins University Use of 8-quinolinol and its analogs to target cancer stem cells

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6365376B1 (en) * 1999-02-19 2002-04-02 E. I. Du Pont De Nemours And Company Genes and enzymes for the production of adipic acid intermediates
US20030113886A1 (en) * 1999-02-19 2003-06-19 Brzostowicz Patricia C. Oxidation of a cyclohexanone derivative using a brevibacterium cyclohexanone monooxygenase
WO2007101867A1 (en) * 2006-03-09 2007-09-13 Basf Se PROCESS FOR THE PRODUCTION OF β-LYSINE
WO2008013966A2 (en) * 2006-07-28 2008-01-31 Johns Hopkins University Use of 8-quinolinol and its analogs to target cancer stem cells

Also Published As

Publication number Publication date
KR20090131073A (en) 2009-12-28
WO2009154378A2 (en) 2009-12-23

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