WO2009145489A3 - 세포투과성 엔도스타틴 재조합 단백질, 이를 코딩하는 폴리뉴클레오티드 및 이를 유효성분으로 함유하는 항암 조성물 - Google Patents

세포투과성 엔도스타틴 재조합 단백질, 이를 코딩하는 폴리뉴클레오티드 및 이를 유효성분으로 함유하는 항암 조성물 Download PDF

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Publication number
WO2009145489A3
WO2009145489A3 PCT/KR2009/001726 KR2009001726W WO2009145489A3 WO 2009145489 A3 WO2009145489 A3 WO 2009145489A3 KR 2009001726 W KR2009001726 W KR 2009001726W WO 2009145489 A3 WO2009145489 A3 WO 2009145489A3
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WIPO (PCT)
Prior art keywords
cell
recombinant protein
same
permeable
cancer
Prior art date
Application number
PCT/KR2009/001726
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English (en)
French (fr)
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WO2009145489A2 (ko
Inventor
조대웅
이종민
박경호
동민탐
Original Assignee
주식회사 프로셀제약
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Application filed by 주식회사 프로셀제약 filed Critical 주식회사 프로셀제약
Priority to US12/936,334 priority Critical patent/US8586544B2/en
Priority to EP09754954A priority patent/EP2270055A4/en
Publication of WO2009145489A2 publication Critical patent/WO2009145489A2/ko
Publication of WO2009145489A3 publication Critical patent/WO2009145489A3/ko

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/78—Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin or cold insoluble globulin [CIG]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Public Health (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Epidemiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Toxicology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

본 발명은 혈관형성 억제인자(angiogenic inhibitor) 엔도스타틴(endostatin)에 거대분자 전달 도메인(macromolecule transduction domain, MTD)이 융합되어 세포투과성을 갖는 엔도스타틴 재조합 단백질, 상기 세포투과성 엔도스타틴 재조합 단백질을 코딩하는 폴리뉴클레오티드, 상기 세포투과성 엔도스타틴 재조합 단백질의 발현벡터, 및 상기 세포투과성 엔도스타틴 재조합 단백질을 유효성분으로 함유하는 암 혈관형성 억제활성이 강화된 항암제용 약학 조성물에 관한 것이다. 본 발명에 따른 세포투과성을 갖는 엔도스타틴 재조합 단백질은 혈관 형성 억제인자 엔도스타틴을 세포 내로 고효율로 도입하여 종양 조직 내에 존재하는 혈관내피세포의 이동, 증식, 침투, 튜브 형성 등을 억제하고 신생 미세혈관의 형성을 차단함으로써 우수한 항암 활성을 나타내므로, 다양한 암에 대한 항암제로서 유용하게 사용될 수 있다.
PCT/KR2009/001726 2008-04-04 2009-04-03 세포투과성 엔도스타틴 재조합 단백질, 이를 코딩하는 폴리뉴클레오티드 및 이를 유효성분으로 함유하는 항암 조성물 WO2009145489A2 (ko)

Priority Applications (2)

Application Number Priority Date Filing Date Title
US12/936,334 US8586544B2 (en) 2008-04-04 2009-04-03 Cell-permeable endostatin recombinant protein, a polynucleotide encoding the same, and an anti-cancer preparation containing the same as an active component
EP09754954A EP2270055A4 (en) 2008-04-04 2009-04-03 CELLPERMEABLE RECOMBINANT ENDOSTATINE PROTEIN, POLYNUCLEOTIDE FOR ITS CODING AND ANTICIPATED PREPARATION WITH THE PROTEIN AS AN ACTIVE SUBSTANCE

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US4231208P 2008-04-04 2008-04-04
US61/042,312 2008-04-04

Publications (2)

Publication Number Publication Date
WO2009145489A2 WO2009145489A2 (ko) 2009-12-03
WO2009145489A3 true WO2009145489A3 (ko) 2010-01-21

Family

ID=41377733

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2009/001726 WO2009145489A2 (ko) 2008-04-04 2009-04-03 세포투과성 엔도스타틴 재조합 단백질, 이를 코딩하는 폴리뉴클레오티드 및 이를 유효성분으로 함유하는 항암 조성물

Country Status (4)

Country Link
US (1) US8586544B2 (ko)
EP (1) EP2270055A4 (ko)
KR (1) KR101263424B1 (ko)
WO (1) WO2009145489A2 (ko)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10647968B2 (en) 2011-09-09 2020-05-12 Tsinghua University Endostatin mutants with mutations at ATP binding sites
WO2013115476A1 (ko) * 2012-01-31 2013-08-08 국립암센터 검체 시료의 응집용 조성물, 이를 이용한 파라핀 블록의 제작방법 및 상기 파라핀 블록을 이용한 세포 마이크로어레이용 세포군집 표본의 제조방법
CN103992400B (zh) * 2014-05-29 2017-01-04 江苏吴中医药集团有限公司苏州中凯生物制药厂 重组人血管内皮抑素的复性液及其制备、使用方法
CN104407153B (zh) * 2014-12-03 2016-04-06 上海交通大学医学院附属上海儿童医学中心 Map2k1作为诊断动脉导管闭合或开放的标志物

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999029855A1 (en) * 1997-12-08 1999-06-17 Beth Israel Deaconess Medical Center Mutants of endostatin, 'em 1' having anti-angiogenic activity and methods of use thereof
WO1999062944A2 (en) * 1998-06-03 1999-12-09 The Children's Medical Center Corporation Protein oligomer compositions comprising endostatin protein and methods of using the same
US20020123458A1 (en) * 1995-10-23 2002-09-05 O'reilly Michael S. Endostatin protein and fragments thereof
WO2003102187A1 (en) * 2002-05-31 2003-12-11 Scegen Pty Ltd Self-coalescing or self-aggregating proteins derived from a membrane translocating sequence
US7470667B2 (en) * 2002-12-05 2008-12-30 Medgenn (Hong Kong) Ltd Methods of treating cancer using a modified endostatin protein

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CA2029273A1 (en) * 1989-12-04 1991-06-05 Christine L. Brakel Modified nucleotide compounds
US6346510B1 (en) * 1995-10-23 2002-02-12 The Children's Medical Center Corporation Therapeutic antiangiogenic endostatin compositions
US5854205A (en) * 1995-10-23 1998-12-29 The Children's Medical Center Corporation Therapeutic antiangiogenic compositions and methods
US6174861B1 (en) * 1996-10-22 2001-01-16 The Children's Medical Center Corporation Methods of inhibiting angiogenesis via increasing in vivo concentrations of endostatin protein
US6852691B1 (en) * 1997-12-08 2005-02-08 Beth Israel Deaconess Medical Center Anti-angiogenic peptides and methods of use thereof
US6797488B1 (en) * 1997-12-08 2004-09-28 Beth Israel Deaconess Medical Center Methods of producing anti-angiogenic proteins
EP1501861A4 (en) * 2002-05-06 2007-06-20 Univ Texas TARGETED BINDING PROTEINS FOR THE ADMINISTRATION OF THERAPEUTIC OR DIAGNOSTIC REAGENTS
US7078485B2 (en) * 2002-12-05 2006-07-18 Yantai Medgenn Ltd. N-terminal modified recombinant human endostatin and its production
AU2008211854C1 (en) 2007-01-29 2014-01-23 Procell Therapeutics Inc. Novel macromolecule transduction domains and methods for identification and uses thereof

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020123458A1 (en) * 1995-10-23 2002-09-05 O'reilly Michael S. Endostatin protein and fragments thereof
WO1999029855A1 (en) * 1997-12-08 1999-06-17 Beth Israel Deaconess Medical Center Mutants of endostatin, 'em 1' having anti-angiogenic activity and methods of use thereof
WO1999062944A2 (en) * 1998-06-03 1999-12-09 The Children's Medical Center Corporation Protein oligomer compositions comprising endostatin protein and methods of using the same
WO2003102187A1 (en) * 2002-05-31 2003-12-11 Scegen Pty Ltd Self-coalescing or self-aggregating proteins derived from a membrane translocating sequence
US7470667B2 (en) * 2002-12-05 2008-12-30 Medgenn (Hong Kong) Ltd Methods of treating cancer using a modified endostatin protein

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of EP2270055A4 *

Also Published As

Publication number Publication date
EP2270055A4 (en) 2011-06-29
KR101263424B1 (ko) 2013-05-14
KR20110013365A (ko) 2011-02-09
EP2270055A2 (en) 2011-01-05
WO2009145489A2 (ko) 2009-12-03
US20110092441A1 (en) 2011-04-21
US8586544B2 (en) 2013-11-19

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