WO2009138855A3 - Methods and systems for the production of granulosa cells - Google Patents

Methods and systems for the production of granulosa cells Download PDF

Info

Publication number
WO2009138855A3
WO2009138855A3 PCT/IB2009/005577 IB2009005577W WO2009138855A3 WO 2009138855 A3 WO2009138855 A3 WO 2009138855A3 IB 2009005577 W IB2009005577 W IB 2009005577W WO 2009138855 A3 WO2009138855 A3 WO 2009138855A3
Authority
WO
WIPO (PCT)
Prior art keywords
granulosa cells
time periods
lif
prolonged time
cultured
Prior art date
Application number
PCT/IB2009/005577
Other languages
French (fr)
Other versions
WO2009138855A2 (en
Inventor
Christian De Geyter
Arnaud Scherberich
Katarzyna Kossowska
Original Assignee
Universitätsspital Basel
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Universitätsspital Basel filed Critical Universitätsspital Basel
Publication of WO2009138855A2 publication Critical patent/WO2009138855A2/en
Publication of WO2009138855A3 publication Critical patent/WO2009138855A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0681—Cells of the genital tract; Non-germinal cells from gonads
    • C12N5/0682—Cells of the female genital tract, e.g. endometrium; Non-germinal cells from ovaries, e.g. ovarian follicle cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0607—Non-embryonic pluripotent stem cells, e.g. MASC
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/23—Interleukins [IL]
    • C12N2501/235—Leukemia inhibitory factor [LIF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/31—Pituitary sex hormones, e.g. follicle-stimulating hormone [FSH], luteinising hormone [LH]; Chorionic gonadotropins

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Reproductive Health (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

The present invention concerns methods and related cultured granulosa cells, assays and kits for drug discovery, therapeutic, and diagnostic purposes based on the discovery that granulosa cells cultured as monolayers remain viable in vitro over prolonged time periods, and exhibit stem cell potential when supplemented with leukaemia-inhibiting factor (LIF), and that use of a three-dimensional culture system such as type I collagen together with the use of LIF allows for both the survival and growth of preantral human GC while supporting a significant subpopulation of GC to maintain their characteristics for prolonged time periods, such as their ability to produce follicle-stimulating hormone receptor and cytochrome P450 aromatase.
PCT/IB2009/005577 2008-05-12 2009-05-12 Methods and systems for the production of granulosa cells WO2009138855A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US5249608P 2008-05-12 2008-05-12
US61/052,496 2008-05-12

Publications (2)

Publication Number Publication Date
WO2009138855A2 WO2009138855A2 (en) 2009-11-19
WO2009138855A3 true WO2009138855A3 (en) 2010-05-20

Family

ID=41258616

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB2009/005577 WO2009138855A2 (en) 2008-05-12 2009-05-12 Methods and systems for the production of granulosa cells

Country Status (1)

Country Link
WO (1) WO2009138855A2 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102361431B1 (en) * 2020-06-12 2022-02-09 이화여자대학교 산학협력단 A method for differentiation of stem cell into estrogen secretory cells
CN114032207A (en) * 2021-08-02 2022-02-11 四川农业大学 Method for separating and culturing swine ovary granular cells
CN113999809A (en) * 2021-12-08 2022-02-01 江苏农牧科技职业学院 In-vitro separation and purification culture method of duck ovarian granulosa cells
CN114438015B (en) * 2022-01-28 2022-11-22 首都医科大学附属北京妇产医院 Human granular cell line from ovary and preparation method and application thereof
WO2024054644A2 (en) * 2022-09-09 2024-03-14 Gameto, Inc. Production and applications of ovarian and uterine organoids

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
DE GEYTER C ET AL: "Luteinizing granulosa cells collected from mature ovarian follicles can be developed into mesenchymal stem cells", HUMAN REPRODUCTION (OXFORD), vol. 22, no. Suppl. 1, July 2007 (2007-07-01), & 23RD ANNUAL MEETING OF THE EUROPEAN-SOCIETY-OF-HUMAN-REPRODUCTION-AND EMBRYOLOGY; LYON, FRANCE; JULY 01 -04, 2007, pages I171 - I172, XP002556209, ISSN: 0268-1161 *
HAIDARI K ET AL: "The effects of different concentrations of leukemia inhibitory factor on the development of isolated preantral follicles from fresh and vitrified mouse ovaries", IRANIAN BIOMEDICAL JOURNAL, PASTEUR INSTITUTE OF IRAN, IRAN, vol. 10, no. 4, 1 October 2006 (2006-10-01), pages 185 - 190, XP009125490, ISSN: 1028-852X *
KOSSOWSKA-TOMASZCZUK KATARZYNA ET AL: "The Multipotency of Luteinizing Granulosa Cells Collected from Mature Ovarian Follicles", STEM CELLS (MIAMISBURG), vol. 27, no. 1, 2009, pages 210 - 219, XP002556210, ISSN: 1066-5099 *

Also Published As

Publication number Publication date
WO2009138855A2 (en) 2009-11-19

Similar Documents

Publication Publication Date Title
WO2011150055A3 (en) Methods, substrates, and systems useful for cell seeding of medical grafts
WO2012056110A3 (en) Cell culture material based on microbial cellulose
EP2588594A4 (en) Methods for culturing undifferentiated cells using sustained release compositions
WO2011139628A8 (en) Generation of anterior foregut endoderm from pluripotent cells
GB2441488A (en) Suspension culture of human embryonic stem cells
MX353245B (en) METHODS FOR REPROGRAMMING CELLS and USES THEREOF.
MX2012000455A (en) Conditioned medium and extracellular matrix compositions from cells cultured under hypoxic conditions.
MX345861B (en) Recombinant laminin-521.
EP2410044A3 (en) Stem cells culture systems
WO2012135621A3 (en) Priming of pluripotent stem cells for neural differentiation
MX356756B (en) Pluripotent stem cell culture on micro-carriers.
EP2633036A4 (en) Differentiation methods for production of glial cell populations
WO2009138855A3 (en) Methods and systems for the production of granulosa cells
GB201111244D0 (en) Culture media for stem cells
GB0916370D0 (en) Compositions
EP3539380A3 (en) Improved cell composition and methods of making the same
WO2012149480A3 (en) Systems and methods for in vitro and in vivo imaging of cells on a substrate
IL212387A0 (en) Perfusion bioreactors, cell culture systems, and methods for production of cells and cell-derived products
MX354068B (en) Bioengineered tissue constructs and methods for producing and using thereof.
EP2588592A4 (en) Use of perfusion decellularized organs for matched recellularization
NZ609731A (en) Compositions of adult organ stem cells and uses thereof
WO2008056368A3 (en) Use of ex-vivo cultured hematopoietic cells for treatment of peripheral vascular diseases
WO2006055841A3 (en) Indefinite culture of human adult glia without immortalization and therapeutic uses thereof
MX343113B (en) Dry granulated cell culture media.
WO2019217493A3 (en) Cell populations and gene expression associated with in vitro beta cell differentiation

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 09746150

Country of ref document: EP

Kind code of ref document: A2

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 09746150

Country of ref document: EP

Kind code of ref document: A2