WO2009130709A3 - Antigène membranaire non recombinant, kit de diagnostic l'employant et procédé de détection de la leishmaniose viscérale et de la pkdl - Google Patents

Antigène membranaire non recombinant, kit de diagnostic l'employant et procédé de détection de la leishmaniose viscérale et de la pkdl Download PDF

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Publication number
WO2009130709A3
WO2009130709A3 PCT/IN2009/000238 IN2009000238W WO2009130709A3 WO 2009130709 A3 WO2009130709 A3 WO 2009130709A3 IN 2009000238 W IN2009000238 W IN 2009000238W WO 2009130709 A3 WO2009130709 A3 WO 2009130709A3
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WO
WIPO (PCT)
Prior art keywords
leishmaniasis
present
pkdl
membrane antigen
serum
Prior art date
Application number
PCT/IN2009/000238
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English (en)
Other versions
WO2009130709A2 (fr
Inventor
Nahid Ali
Samiran Saha
Original Assignee
Council Of Scientific & Industrial Research
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Council Of Scientific & Industrial Research filed Critical Council Of Scientific & Industrial Research
Publication of WO2009130709A2 publication Critical patent/WO2009130709A2/fr
Publication of WO2009130709A3 publication Critical patent/WO2009130709A3/fr

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54386—Analytical elements
    • G01N33/54387—Immunochromatographic test strips
    • G01N33/54388—Immunochromatographic test strips based on lateral flow
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/44—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from protozoa
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56905—Protozoa
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/44—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans from protozoa

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Organic Chemistry (AREA)
  • Virology (AREA)
  • Toxicology (AREA)
  • Zoology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Peptides Or Proteins (AREA)

Abstract

La présente invention concerne un antigène membranaire non recombinant (LAg) directement obtenu à partir de promastigotes de Leishmania donovani souche AG83. L'antigène membranaire (LAg) a les caractéristiques suivantes d'un complexe de 25-35 polypeptides et une masse moléculaire dans la plage de 18 - 155 KD. Ladite membrane est sensible et spécifique jusqu'à la plage de 95 % à 100 % aux anticorps IgG anti-Leishmania dans le sérum d'un patient soufrant de leishmaniose viscérale (VL) ou de leishmaniose cutanée post kala-azar (PKDL). La présente invention concerne aussi un procédé de préparation dudit antigène membranaire et son procédé d'utilisation pour la détection d'anticorps IgG anti-Leishmania présents dans le sérum d'un patient soufrant de leishmaniose viscérale (VL) ou de leishmaniose cutanée post kala-azar (PKDL). La présente invention concerne en outre un kit de diagnostic comprenant ledit antigène membranaire non recombinant, utile pour détecter des anticorps IgG anti-Leishmania présents dans le sérum d'un patient soufrant de leishmaniose viscérale (VL) ou de leishmaniose cutanée post kala-azar (PKDL). La présente invention concerne également un procédé de détection et de quantification d'anticorps IgG anti-Leishmania présents dans le sérum de patients soufrant de leishmaniose viscérale (VL) ou de leishmaniose cutanée post kala-azar (PKDL) au moyen du kit de diagnostic.
PCT/IN2009/000238 2008-04-23 2009-04-17 Antigène membranaire non recombinant, kit de diagnostic l'employant et procédé de détection de la leishmaniose viscérale et de la pkdl WO2009130709A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IN1039DE2008 2008-04-23
IN1039/DEL/2008 2008-04-23

Publications (2)

Publication Number Publication Date
WO2009130709A2 WO2009130709A2 (fr) 2009-10-29
WO2009130709A3 true WO2009130709A3 (fr) 2010-03-11

Family

ID=40935675

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IN2009/000238 WO2009130709A2 (fr) 2008-04-23 2009-04-17 Antigène membranaire non recombinant, kit de diagnostic l'employant et procédé de détection de la leishmaniose viscérale et de la pkdl

Country Status (1)

Country Link
WO (1) WO2009130709A2 (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10422806B1 (en) * 2013-07-25 2019-09-24 Theranos Ip Company, Llc Methods for improving assays of biological samples
WO2016113749A1 (fr) * 2015-01-13 2016-07-21 Council Of Scientific & Industrial Research Kit utile pour mesurer des anticorps dirigés contre un antigène membranaire non recombiné (lag) dans un échantillon d'urine

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0293827A2 (fr) * 1987-06-01 1988-12-07 Yeda Research And Development Company, Ltd. Essai pour leishmaniose

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0293827A2 (fr) * 1987-06-01 1988-12-07 Yeda Research And Development Company, Ltd. Essai pour leishmaniose

Non-Patent Citations (8)

* Cited by examiner, † Cited by third party
Title
AFRIN ET AL: "Adjuvancity and protective immunity elicted by Leishmania donovani antigens encapsulated in positively charged liposomes", INFECTION AND IMMUNITY, AMERICAN SOCIETY FOR MICROBIOLOGY, WASHINGTON, US, vol. 65, no. 6, 1 June 1997 (1997-06-01), pages 2371 - 2377, XP002991927, ISSN: 0019-9567 *
ANAM K ET AL: "Immunoglobulin subclass distribution and diagnostic value of Leishmania donovani antigen-specific immunoglobulin G3 in Indian kala-azar patients.", CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY MAR 1999, vol. 6, no. 2, March 1999 (1999-03-01), pages 231 - 235, XP002541685, ISSN: 1071-412X *
BANDYOPADHYAY S ET AL: "Purification, characterization of O-acetylated sialoglycoconjugates-specific IgM, and development of an enzyme-linked immunosorbent assay for diagnosis and follow-up of indian visceral leishmaniasis patients", DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASES, ELSEVIER SCIENCE PUBLISHING CO., AMSTERDAM, NL, vol. 50, no. 1, 1 September 2004 (2004-09-01), pages 15 - 24, XP004565933, ISSN: 0732-8893 *
MARTIN S K ET AL: "A DIAGNOSTIC ELISA FOR VISCERAL LEISHMANIASIS, BASED ON ANTIGEN FROM MEDIA CONDITIONED BY LEISHMANIA DONOVANI PROMASTIGOTES", ANNALS OF TROPICAL MEDICINE AND PARASITOLOGY, ACADEMIC PRESS, LONDON, GB, vol. 92, no. 5, 1 January 1998 (1998-01-01), pages 571 - 577, XP002942877, ISSN: 0003-4983 *
MUKERJI K ET AL: "Direct enzyme-linked immunosorbent assay: a simple immunoassay using Leishmania donovani promastigote for diagnosis of kala-azar.", JOURNAL OF CLINICAL LABORATORY ANALYSIS 1991, vol. 5, no. 4, 1991, pages 299 - 301, XP002942878, ISSN: 0887-8013 *
SAHA SAMIRAN ET AL: "Immune responses in kala-azar.", THE INDIAN JOURNAL OF MEDICAL RESEARCH MAR 2006, vol. 123, no. 3, March 2006 (2006-03-01), pages 245 - 266, XP002541684, ISSN: 0971-5916 *
SAHA SAMIRAN ET AL: "Leishmania promastigote membrane antigen-based enzyme-linked immunosorbent assay and immunoblotting for differential diagnosis of Indian post-kala-azar dermal leishmaniasis.", JOURNAL OF CLINICAL MICROBIOLOGY MAR 2005, vol. 43, no. 3, March 2005 (2005-03-01), pages 1269 - 1277, XP002541682, ISSN: 0095-1137 *
TRULLOLS E ET AL: "Validation of qualitative analytical methods", TRAC, TRENDS IN ANALYTICAL CHEMISTRY, ELSEVIER, AMSTERDAM, NL, vol. 23, no. 2, 1 February 2004 (2004-02-01), pages 137 - 145, XP004566146, ISSN: 0165-9936 *

Also Published As

Publication number Publication date
WO2009130709A2 (fr) 2009-10-29

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