WO2009128644A3 - 게놈 수준에서의 부탄올 생산 미생물의 대사 네트워크 모델 및 이를 이용한 부탄올 생성 미생물의 대사특성분석 및 결실 표적 스크리닝 방법 - Google Patents

게놈 수준에서의 부탄올 생산 미생물의 대사 네트워크 모델 및 이를 이용한 부탄올 생성 미생물의 대사특성분석 및 결실 표적 스크리닝 방법 Download PDF

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Publication number
WO2009128644A3
WO2009128644A3 PCT/KR2009/001923 KR2009001923W WO2009128644A3 WO 2009128644 A3 WO2009128644 A3 WO 2009128644A3 KR 2009001923 W KR2009001923 W KR 2009001923W WO 2009128644 A3 WO2009128644 A3 WO 2009128644A3
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WIPO (PCT)
Prior art keywords
metabolic
butanol
analysis
network model
producing
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PCT/KR2009/001923
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English (en)
French (fr)
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WO2009128644A9 (ko
WO2009128644A2 (ko
Inventor
이상엽
이종민
윤홍석
정광섭
Original Assignee
한국과학기술원
바이오퓨얼켐 주식회사
지에스칼텍스 주식회사
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Publication of WO2009128644A2 publication Critical patent/WO2009128644A2/ko
Publication of WO2009128644A3 publication Critical patent/WO2009128644A3/ko
Publication of WO2009128644A9 publication Critical patent/WO2009128644A9/ko

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C12Q1/32—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving dehydrogenase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/16—Butanols
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/527—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving lyase
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Physics & Mathematics (AREA)
  • General Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Cell Biology (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

본 발명은 부탄올 생성 미생물의 대사 특성 분석용 대사 네트워크 모델 및 이를 이용한 부탄올 생성 미생물의 대사특성분석 및 결실 표적 스크리닝 방법에 관한 것으로, 보다 상세하게는 유전자-단백질-생화학 반응관계를 이용한 부탄올 생성 미생물의 대사 네트워크 모델의 구축 및 구축된 모델을 이용한 대사흐름 등 대사특성의 분석과, 대사흐름분석에 기반한 시뮬레이션을 이용하여 결실 시 특정 대사산물의 생성능을 향상시키는 결실 표적을 스크리닝하는 방법에 관한 것이다. 본 발명에 따른 대사 네트워크 모델은 부탄올 생성 미생물의 대사흐름 등 대사특성 분석 및 특정 대사산물의 생산을 증가시키기 위한 결실 표적 효소 또는 그 유전자의 스크리닝에 유용하며, 본 발명에 따른 결실 표적 스크리닝 방법은 기존의 방법과 달리, 효율적으로 결실 표적들을 예측할 수 있게 하며, 시간 및 비용을 절약할 수 있게 하고, 특정 대사산물을 고효율로 생산할 수 있는 변이 미생물을 제공하는 효과가 있으므로, 유용하다.
PCT/KR2009/001923 2008-04-14 2009-04-14 게놈 수준에서의 부탄올 생산 미생물의 대사 네트워크 모델 및 이를 이용한 부탄올 생성 미생물의 대사특성분석 및 결실 표적 스크리닝 방법 WO2009128644A2 (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR20080034238 2008-04-14
KR10-2008-0034238 2008-04-14

Publications (3)

Publication Number Publication Date
WO2009128644A2 WO2009128644A2 (ko) 2009-10-22
WO2009128644A3 true WO2009128644A3 (ko) 2010-01-21
WO2009128644A9 WO2009128644A9 (ko) 2010-04-15

Family

ID=41199565

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2009/001923 WO2009128644A2 (ko) 2008-04-14 2009-04-14 게놈 수준에서의 부탄올 생산 미생물의 대사 네트워크 모델 및 이를 이용한 부탄올 생성 미생물의 대사특성분석 및 결실 표적 스크리닝 방법

Country Status (2)

Country Link
KR (1) KR101100866B1 (ko)
WO (1) WO2009128644A2 (ko)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105603035B (zh) * 2014-11-19 2021-05-07 巴斯夫欧洲公司 为增加imp脱氢酶活性的假囊酵母属的遗传修饰

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* Cited by examiner, † Cited by third party
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DE102011006716A1 (de) * 2011-04-04 2012-10-04 Evonik Degussa Gmbh Mikroorganismus und Verfahren zur fermentativen Herstellung einer organisch-chemischen Verbindung
KR20240023684A (ko) 2016-09-13 2024-02-22 알레간 인코포레이티드 안정화된 비단백질 클로스트리듐 독소 조성물
CN106947717A (zh) * 2017-04-14 2017-07-14 江南大学 一种以猪粪为原料培养植物促生细菌制备稀土尾矿改良菌剂的技术
CN107988242B (zh) * 2017-12-14 2021-05-11 大连理工大学 manY/levF基因片段在生产丁醇中的应用
CN111887473B (zh) * 2020-07-14 2022-07-01 云南省烟草农业科学研究院 基于全基因组选择烟气有害成分释放量预测方法及应用
CN114657217A (zh) * 2020-12-22 2022-06-24 安徽华恒生物科技股份有限公司 一种高效的d-泛解酸的制备方法
CN114657216A (zh) * 2020-12-22 2022-06-24 安徽华恒生物科技股份有限公司 一种d-泛解酸及其制备方法和其应用
CN115011646A (zh) * 2022-07-11 2022-09-06 宁夏华吉生物有限公司 一种利用鸟苷制备鸟嘌呤和d-核糖的方法

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5831993A (ja) * 1981-08-20 1983-02-24 Idemitsu Kosan Co Ltd ブタノ−ルの製造法
EP0125983A2 (en) * 1983-05-10 1984-11-21 National Aeronautics And Space Administration Production of butanol by fermentation in the presence of cocultures of clostridium
US20070190605A1 (en) * 2004-06-29 2007-08-16 Cornelius Bessler Gene products of bacillus licheniformis which form odorous substances and improved biotechnological production methods based thereon
WO2007130521A2 (en) * 2006-05-02 2007-11-15 E. I. Du Pont De Nemours And Company Fermentive production of four carbon alcohols

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008072920A1 (en) * 2006-12-15 2008-06-19 Biofuelchem Co., Ltd. Method for preparing butanol through butyryl-coa as an intermediate using bacteria

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5831993A (ja) * 1981-08-20 1983-02-24 Idemitsu Kosan Co Ltd ブタノ−ルの製造法
EP0125983A2 (en) * 1983-05-10 1984-11-21 National Aeronautics And Space Administration Production of butanol by fermentation in the presence of cocultures of clostridium
US20070190605A1 (en) * 2004-06-29 2007-08-16 Cornelius Bessler Gene products of bacillus licheniformis which form odorous substances and improved biotechnological production methods based thereon
WO2007130521A2 (en) * 2006-05-02 2007-11-15 E. I. Du Pont De Nemours And Company Fermentive production of four carbon alcohols

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
LEE, SANG-YUP: "Microbial Biotechnology Powered by Genomics, Proteomics,Metabolomics and Bioinformatics", THE KOREAN SOCIETY FOR BIOINFORMATICS AND SYSTEMS BIOLOGY INTERNATIONAL SYMPOSIUM ON BIOINFORMATICS, - 1 November 2000 (2000-11-01), pages 13 - 16 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105603035B (zh) * 2014-11-19 2021-05-07 巴斯夫欧洲公司 为增加imp脱氢酶活性的假囊酵母属的遗传修饰

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WO2009128644A9 (ko) 2010-04-15
WO2009128644A2 (ko) 2009-10-22
KR101100866B1 (ko) 2012-01-02
KR20090109072A (ko) 2009-10-19

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