WO2009108223A2 - Détection d’agents pathogènes par mesure simultanée de la taille/de la fluorescence - Google Patents
Détection d’agents pathogènes par mesure simultanée de la taille/de la fluorescence Download PDFInfo
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- WO2009108223A2 WO2009108223A2 PCT/US2008/083052 US2008083052W WO2009108223A2 WO 2009108223 A2 WO2009108223 A2 WO 2009108223A2 US 2008083052 W US2008083052 W US 2008083052W WO 2009108223 A2 WO2009108223 A2 WO 2009108223A2
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- particle
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Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Electro-optical investigation, e.g. flow cytometers
- G01N15/1456—Electro-optical investigation, e.g. flow cytometers without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
- G01N15/1459—Electro-optical investigation, e.g. flow cytometers without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals the analysis being performed on a sample stream
-
- G01N2015/019—
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Electro-optical investigation, e.g. flow cytometers
- G01N2015/1493—Particle size
Abstract
La présente invention concerne un procédé et un appareil permettant de détecter des agents pathogènes et des particules dans un fluide. Dans ledit procédé et ledit appareil, il est possible de déterminer la taille particulaire et la fluorescence intrinsèque d’une particule. La présente invention concerne la classification de particules en tant que particules biologiques ou inertes au moyen d’un ou de plusieurs seuils.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US269207P | 2007-11-09 | 2007-11-09 | |
US61/002,692 | 2007-11-09 |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2009108223A2 true WO2009108223A2 (fr) | 2009-09-03 |
WO2009108223A3 WO2009108223A3 (fr) | 2010-01-21 |
Family
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Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US2008/083052 WO2009108223A2 (fr) | 2007-11-09 | 2008-11-10 | Détection d’agents pathogènes par mesure simultanée de la taille/de la fluorescence |
Country Status (1)
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WO (1) | WO2009108223A2 (fr) |
Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20130030715A1 (en) * | 2011-07-29 | 2013-01-31 | Honeywell Asca Inc. | Apparatus and method for monitoring autotroph cultivation |
EP2610832A3 (fr) * | 2011-12-27 | 2014-03-12 | Agency For Defense Development | Procédé de génération d'alarme dynamique de surveillance de particules biologiques |
EP2960640A1 (fr) * | 2014-06-26 | 2015-12-30 | Azbil Corporation | Dispositif de detection de particules et procede de detection de particules |
CN105203445A (zh) * | 2014-06-26 | 2015-12-30 | 阿自倍尔株式会社 | 粒子检测装置及粒子检测方法 |
WO2017056592A1 (fr) * | 2015-10-01 | 2017-04-06 | シャープ株式会社 | Dispositif de détection de fluorescence |
CN109074033A (zh) * | 2016-04-01 | 2018-12-21 | 凯米罗总公司 | 用于优化水处理过程中的凝聚和/或絮凝的方法和系统 |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5290707A (en) * | 1991-11-25 | 1994-03-01 | The United States Of America As Represented By The Secretary Of The Army | Method for detection of microorganisms |
US5540494A (en) * | 1994-06-03 | 1996-07-30 | Purvis, Jr.; Norman B. | Method and apparatus for determining absolute particle size, surface area and volume normalized fluorescence using forward angle light scatter intensity in flow cytometry |
WO2005033283A2 (fr) * | 2003-09-30 | 2005-04-14 | Singulex, Inc. | Procedes pour ameliorer l'analyse de detection de particules |
US20050221497A1 (en) * | 1992-02-24 | 2005-10-06 | Carole Young | Quality control method |
-
2008
- 2008-11-10 WO PCT/US2008/083052 patent/WO2009108223A2/fr active Application Filing
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5290707A (en) * | 1991-11-25 | 1994-03-01 | The United States Of America As Represented By The Secretary Of The Army | Method for detection of microorganisms |
US20050221497A1 (en) * | 1992-02-24 | 2005-10-06 | Carole Young | Quality control method |
US5540494A (en) * | 1994-06-03 | 1996-07-30 | Purvis, Jr.; Norman B. | Method and apparatus for determining absolute particle size, surface area and volume normalized fluorescence using forward angle light scatter intensity in flow cytometry |
WO2005033283A2 (fr) * | 2003-09-30 | 2005-04-14 | Singulex, Inc. | Procedes pour ameliorer l'analyse de detection de particules |
Non-Patent Citations (4)
Title |
---|
HAIRSTON ET AL.: 'Design of an Instrument for Real-Time Detection of Bioaerosols Using Simultaneous Measurement of Particle Aerodynamic Size and Intrinsic Fluorescence.' JOURNAL OF AEROSOL SCIENCE vol. 28, no. 3, 1997, pages 471 - 482 * |
'Searched for http://www.biovigilant.com/PDFs/Why Simultaneous Sizing and Fluorescence is Necessary for Microbial Detection 9-8-05-c.pdf.' WAYBACK MACHINE INTERNET ARCHIVE., [Online] 10 July 2009, Retrieved from the Internet: <URL:http://web.archive.org/web/"/http://ww w.biovigilant.com/PDFs/Why%20Simultaneous%2 0Sizing %20and%20Fluorescence%20is%20Necessary%20fo r%20Microbial%20Detection%209-8-05- c.pdf> [retrieved on 2009-07-10] * |
WEICHERT ET AL.: 'Determination of Fluorescence Cross-Sections of Biological Aerosols.' PARTICLE & PARTICLE SYSTEMS CHARACTERIZATION vol. 19, no. ISS.3, 01 July 2002, pages 216 - 222 * |
'Why Simultaneous Sizing and Fluorescence is Necessary in Order to Do Microbial Detection.' BIOVIGILANT.COM BIOVIGILANT WHITE PAPER, [Online] 21 October 2006, Retrieved from the Internet: <URL:http://www.biovigilant.com/PDFs/Why%20 Simultaneous%20Sizing%20and%20Fluorescence% 20is%20Necessary%20for%20Microbial%20Detect ion%209-8-05-c.pdf> [retrieved on 2009-07-06] * |
Cited By (13)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20130030715A1 (en) * | 2011-07-29 | 2013-01-31 | Honeywell Asca Inc. | Apparatus and method for monitoring autotroph cultivation |
US9540604B2 (en) * | 2011-07-29 | 2017-01-10 | Honeywell Limited | Apparatus and method for monitoring autotroph cultivation |
EP2610832A3 (fr) * | 2011-12-27 | 2014-03-12 | Agency For Defense Development | Procédé de génération d'alarme dynamique de surveillance de particules biologiques |
CN105203445A (zh) * | 2014-06-26 | 2015-12-30 | 阿自倍尔株式会社 | 粒子检测装置及粒子检测方法 |
CN105223112A (zh) * | 2014-06-26 | 2016-01-06 | 阿自倍尔株式会社 | 粒子检测装置及粒子检测方法 |
JP2016008957A (ja) * | 2014-06-26 | 2016-01-18 | アズビル株式会社 | 粒子検出装置及び粒子の検出方法 |
EP2960640A1 (fr) * | 2014-06-26 | 2015-12-30 | Azbil Corporation | Dispositif de detection de particules et procede de detection de particules |
US9671346B2 (en) | 2014-06-26 | 2017-06-06 | Azbil Corporation | Particle detecting and discriminating device and method |
CN105203445B (zh) * | 2014-06-26 | 2019-08-06 | 阿自倍尔株式会社 | 粒子检测装置及粒子检测方法 |
WO2017056592A1 (fr) * | 2015-10-01 | 2017-04-06 | シャープ株式会社 | Dispositif de détection de fluorescence |
CN109074033A (zh) * | 2016-04-01 | 2018-12-21 | 凯米罗总公司 | 用于优化水处理过程中的凝聚和/或絮凝的方法和系统 |
US10988392B2 (en) | 2016-04-01 | 2021-04-27 | Kemira Oyj | Method and system for optimization of coagulation and/or flocculation in a water treatment process |
CN109074033B (zh) * | 2016-04-01 | 2022-04-19 | 凯米罗总公司 | 用于优化水处理过程中的凝聚和/或絮凝的方法和系统 |
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WO2009108223A3 (fr) | 2010-01-21 |
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