WO2009097099A1 - Dispositif microfluidique pour la culture de cellules - Google Patents

Dispositif microfluidique pour la culture de cellules Download PDF

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Publication number
WO2009097099A1
WO2009097099A1 PCT/US2009/000459 US2009000459W WO2009097099A1 WO 2009097099 A1 WO2009097099 A1 WO 2009097099A1 US 2009000459 W US2009000459 W US 2009000459W WO 2009097099 A1 WO2009097099 A1 WO 2009097099A1
Authority
WO
WIPO (PCT)
Prior art keywords
micro
microfluidic
wells
membranes
base plate
Prior art date
Application number
PCT/US2009/000459
Other languages
English (en)
Inventor
Jiang Huang
Original Assignee
Gn Biosystems Incorporated
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gn Biosystems Incorporated filed Critical Gn Biosystems Incorporated
Publication of WO2009097099A1 publication Critical patent/WO2009097099A1/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/16—Microfluidic devices; Capillary tubes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/12—Well or multiwell plates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/02—Membranes; Filters
    • C12M25/04—Membranes; Filters in combination with well or multiwell plates, i.e. culture inserts

Definitions

  • FIG. 2 illustrates a cross-sectional view of a portion of microfluidic device 100 taken along axis Y1-Y2 of FIG. 1 , in accordance with an embodiment of the invention.
  • a microfluidic base plate 201 is placed below microtiter plate 101 in a manner such that a micro-porous membrane 202 is between microtiter plate 101 and microfluidic base plate 201.
  • micro-well 302 may have any other suitable shape, area and/or dimensions.
  • section 30Od includes micro-well 303.
  • micro-well 303 is shown to have a rectangular cross section.
  • micro-well 303 is 0.01 mm to 10 mm wide, 0.01 mm to 10 mm long and 0.01 mm to 5 mm deep.
  • micro-well 303 may have any other appropriate shape, area and/or dimensions.
  • micro-wells 302 and 303 may be identical.
  • micro-well 401 may be used to add reagents, culture media, nutrients, drug candidates or other molecules to micro-well 402 in a similar manner.
  • FIG. 5 illustrates the top view of microfluidic base plate 201 of microfluidic device 100 in accordance with another embodiment of the invention.
  • Microfluidic base plate 201 is placed below microtiter plate 101 , shown in FIG. 1 , in a manner such that micro- porous membrane 202 is present between microfluidic base plate 201 and microtiter plate 101.
  • microtiter plate 101 and micro-porous membrane 202 are not shown in FIG. 5.
  • Sections 500a and 500b of microfluidic base plate 201 lie under the wells of microtiter plate 101. According to the embodiment, sections 500a and 500b form a unit cell 501 of microfluidic base plate 201.
  • microfluidic base plate 201 is shown to include twelve unit cells. In other embodiments of the invention, microfluidic base plate 201 may include any number of unit cells.
  • Microfluidic base plate 201 may be micro-fabricated to include micro-wells 502 and 503.
  • Examples of the technologies used for micro-fabrication include micromachining, hot embossing, injection molding of plastics, photolithography, Deep Reactive Ion Etching (DRIE), and electrochemistry.
  • DRIE Deep Reactive Ion Etching
  • Opening 605 may be created by rupturing micro-porous membrane 604 by using a pointed article.
  • micro-porous membrane 604 may be pre-fabricated to include opening 605.
  • Micro-well 602 may be used to culture cells. The cells to be cultured are introduced into micro-well 601 , for example, by pipetting. These cells may be forced to move to micro-well 602 through micro-channel 603 by using techniques such as pneumatic pressure, centrifugal force, capillary flow, etc. In another embodiment, the cells may diffuse to micro-well 602 from micro-well 601 via micro-channel 603.
  • micro- channel 403 may also be used to remove the cell culture products and/or waste products from micro-well 402.
  • the cell culture products and/or waste products may diffuse out of micro-well 402 through micro-channel 403 and can be collected at micro- well 401.
  • the device may be used to study complex biological reactions, such as cell signaling, drug screening, cell- differentiation studies, cell-based biosensors, cell-cell interaction, cell-invasion and cell-migration studies, chemotaxis studies, drug transport assays, physiological and pathophysiological studies, etc.
  • complex biological reactions such as cell signaling, drug screening, cell- differentiation studies, cell-based biosensors, cell-cell interaction, cell-invasion and cell-migration studies, chemotaxis studies, drug transport assays, physiological and pathophysiological studies, etc.

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Clinical Laboratory Science (AREA)
  • Immunology (AREA)
  • Dispersion Chemistry (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

L’invention concerne un système microfluidique et un procédé pour la culture de cellules. Le système microfluidique comprend une plaque de base microfluidique comprenant une pluralité de micropuits. Certains de ces micropuits sont recouverts d’une membrane microporeuse. En outre, au moins un micropuits est relié à au moins un autre micropuits par un ou plusieurs microcanaux. Une plaque de microtitration ouverte, comprenant une multitude de puits, est attachée à la plaque de base microfluidique de telle sorte que la membrane microporeuse est placée entre la plaque de microtitration et la plaque de base microfluidique. Le procédé décrit consiste à introduire des cellules et des liquides dans les micropuits par une ouverture dans la membrane microporeuse, et laisser les cellules et les liquides se diffuser entre les micropuits par les microcanaux.
PCT/US2009/000459 2008-01-29 2009-01-23 Dispositif microfluidique pour la culture de cellules WO2009097099A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US6273808P 2008-01-29 2008-01-29
US61/062,738 2008-01-29

Publications (1)

Publication Number Publication Date
WO2009097099A1 true WO2009097099A1 (fr) 2009-08-06

Family

ID=40913138

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2009/000459 WO2009097099A1 (fr) 2008-01-29 2009-01-23 Dispositif microfluidique pour la culture de cellules

Country Status (1)

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WO (1) WO2009097099A1 (fr)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011161480A1 (fr) 2010-06-25 2011-12-29 Imperial Innovations Ltd Plaque d'essai multi-puits
WO2013116449A1 (fr) * 2012-02-02 2013-08-08 Corning Incorporated Consommables de microplaque de culture cellulaire à perfusion continue automatique
WO2015142462A1 (fr) * 2014-03-21 2015-09-24 Iontox, Llc Plaque dynamique multi-organes
CN107478851A (zh) * 2017-08-23 2017-12-15 成都和合医学检验所有限公司 快速定量检测人体血液中25‑羟基维生素d3含量的方法
WO2018005389A1 (fr) * 2016-06-30 2018-01-04 General Automation Lab Technologies, Inc. Systèmes à haute résolution, trousses, appareil, et procédés utilisant des stratégies de supports combinatoires pour des applications de microbiologie à débit élevé.

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6943009B2 (en) * 2002-05-15 2005-09-13 Corning Incorporated Multi-well assembly for growing cultures in-vitro
US20070166816A1 (en) * 2002-03-12 2007-07-19 Surface Logix, Inc. Assay device that analyzes the absorption, metabolism, permeability and/or toxicity of a candidate compound

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070166816A1 (en) * 2002-03-12 2007-07-19 Surface Logix, Inc. Assay device that analyzes the absorption, metabolism, permeability and/or toxicity of a candidate compound
US6943009B2 (en) * 2002-05-15 2005-09-13 Corning Incorporated Multi-well assembly for growing cultures in-vitro

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011161480A1 (fr) 2010-06-25 2011-12-29 Imperial Innovations Ltd Plaque d'essai multi-puits
WO2013116449A1 (fr) * 2012-02-02 2013-08-08 Corning Incorporated Consommables de microplaque de culture cellulaire à perfusion continue automatique
WO2015142462A1 (fr) * 2014-03-21 2015-09-24 Iontox, Llc Plaque dynamique multi-organes
US9631167B2 (en) 2014-03-21 2017-04-25 Iontox, Llc Dynamic multi organ plate
WO2018005389A1 (fr) * 2016-06-30 2018-01-04 General Automation Lab Technologies, Inc. Systèmes à haute résolution, trousses, appareil, et procédés utilisant des stratégies de supports combinatoires pour des applications de microbiologie à débit élevé.
CN107478851A (zh) * 2017-08-23 2017-12-15 成都和合医学检验所有限公司 快速定量检测人体血液中25‑羟基维生素d3含量的方法

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