WO2009086264A1 - Benzofuropyrimidinones as protein kinase inhibitors - Google Patents

Benzofuropyrimidinones as protein kinase inhibitors Download PDF

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Publication number
WO2009086264A1
WO2009086264A1 PCT/US2008/087939 US2008087939W WO2009086264A1 WO 2009086264 A1 WO2009086264 A1 WO 2009086264A1 US 2008087939 W US2008087939 W US 2008087939W WO 2009086264 A1 WO2009086264 A1 WO 2009086264A1
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WIPO (PCT)
Prior art keywords
pyrimidin
bromo
benzofuro
methyl
alkyl
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PCT/US2008/087939
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French (fr)
Inventor
S. David Brown
Hongwang Du
Maurizio Franzini
Adam Antoni Galan
Ping Huang
Patrick Kearney
Moon Hwan Kim
Elena S. Koltun
Steven James Richards
Amy L. Tsuhako
Cristiana A. Zaharia
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Exelixis, Inc.
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Priority to JP2010539930A priority Critical patent/JP2011507908A/en
Priority to EP08832770A priority patent/EP2097419B1/en
Priority to MX2010006799A priority patent/MX2010006799A/en
Priority to CA2712358A priority patent/CA2712358A1/en
Priority to AU2008345560A priority patent/AU2008345560A1/en
Application filed by Exelixis, Inc. filed Critical Exelixis, Inc.
Priority to EA201070786A priority patent/EA019785B1/en
Priority to NZ586936A priority patent/NZ586936A/en
Priority to BRPI0821573A priority patent/BRPI0821573A2/en
Priority to AT08832770T priority patent/ATE510839T1/en
Priority to CN2008801272275A priority patent/CN101965351A/en
Publication of WO2009086264A1 publication Critical patent/WO2009086264A1/en
Priority to ZA2010/04243A priority patent/ZA201004243B/en
Priority to IL206416A priority patent/IL206416A0/en

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D491/00—Heterocyclic compounds containing in the condensed ring system both one or more rings having oxygen atoms as the only ring hetero atoms and one or more rings having nitrogen atoms as the only ring hetero atoms, not provided for by groups C07D451/00 - C07D459/00, C07D463/00, C07D477/00 or C07D489/00
    • C07D491/02—Heterocyclic compounds containing in the condensed ring system both one or more rings having oxygen atoms as the only ring hetero atoms and one or more rings having nitrogen atoms as the only ring hetero atoms, not provided for by groups C07D451/00 - C07D459/00, C07D463/00, C07D477/00 or C07D489/00 in which the condensed system contains two hetero rings
    • C07D491/04—Ortho-condensed systems
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A61P35/02—Antineoplastic agents specific for leukemia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00

Definitions

  • This disclosure relates to certain benzofuropyrimidinone compounds.
  • this disclosure relates to certain benzofuropyrimidinone compounds useful as inhibitors of protein kinases.
  • PIM protein kinase family which consists of the closely related PIM- 1, 2, and 3, have been implicated in diverse biological processes such as cell survival, proliferation, and differentiation.
  • PIM-I is involved in a number of signaling pathways that are highly relevant to tumorigenesis [reviewed in Bachmann & Moray, Internat. J. Biochem. Cell Biol, 37, 726-730 (2005)]. Many of these are involved in cell cycle progression and apoptosis. It has been shown that PIM-I acts as an anti- apoptotic factor via inactivation of the pro-apoptotic factor Bad.
  • PIM-I has also been recognized as a positive regulator of cell cycle progression. PIM-I binds and phosphorylates CDC25A, which leads to an increase in its phosphatase activity and promotion of Gl /S transition [reviewed in Losman et al., JBC, 278, 4800-4805 (1999)].
  • cyclin kinase inhibitor p21 Waf which inhibits Gl /S progression was found to be inactivated by PIM-I [Wang et al., Biochim. Biophys. Act. 1593, 45-55 (2002)]. Furthermore, by means of phosphorylation, Pim-1 inactivates C-TAKl and activates Cdc25C which results in acceleration of G2/M transition [Bachman et al., JBC, 279, 48319-48 (2004)]. [0004] PIM-I appears to be an essential player in hematopoetic proliferation. Kinase active PIM-I is required for the gpl30-mediated STAT3 proliferation signal [Hirano et al., Oncogene 19, 2548-2556, (2000)]
  • PIM-I is overexpressed or even mutated in a number of tumors and different types of tumor cell lines and leads to genomic instability.
  • Examples for a possible involvement of PIM-I in human tumors are prostate cancer, oral cancer, and Burkitt lymphoma (Gaidano & Dalla Faver, 1993). All these findings point to an important role of PIM-I in the initiation and progression of human cancer, and it appears that small molecule inhibition of PIM-I activity is a promising therapeutic strategy.
  • PIM -2 and PIM-3 have overlapping functions with PIM-I and inhibition of more than one isoform may provide additional therapeutic benefits.
  • CDC7 a serine/threonine kinase
  • MCM minichromosome maintenance
  • CDC7 requires association with one of its co factors, ASK (also known as DBF4) or ASKLl (also known as Drfl), for kinase activation (Ogino et al., J Biol Chem 276:31376 (2001); Sato et al., Genes to Cells 8:451 (2003); Montagnoli et al., EMBO J 21 :3171 (2002); Yoshizawa-Sugata et al., J Biol Chem 280, 13062 (2005)). Mice deficient for CDC7 die between day 3.5 and 6.5 indicating that CDC7 plays a role for early embryonic development (Kim et al., EMBO J 21 :2168 (2002)).
  • CDC7-/-tg Conditional knock-down of CDC7 in mouse ES cell lines revealed immediate inhibition of cell proliferation, rapid cessation of DNA synthesis and arrest in S phase progression (Kim et al. (2002)).
  • CDC7 has been implicated in DNA damage checkpoint signaling in response to Etoposide treatment or DNA single strand breaks (Costanzo et al., J MoI Cell 11 :203 (2003)).
  • a role for CDC7 in DNA damage response is supported by the observation that CDC7 depleted mouse ES cells accumulate RAD51 foci in the nucleus (Kim et al. (2002)). Deletion of CDC7 in yeast results in hypersensitivity to hydroxyurea treatment (Weinheim et al., EMBO J 18:5334 (1999)).
  • the serine/threonine kinase CDC7 plays an important role in the initiation of DNA replication and recently has been implicated in S phase checkpoint signaling(reviewed in Kim, Yamada and Masai, "Functions of mammalian CDC7 kinase in initiation/monitoring of DNA replication and development" Mutat Res 532(l-2):29-40 (2003)).
  • the CDC7 kinase forms a complex with Dbf4, its regulatory subunit also known as ASK to generate an active Ser/Thr kinase.
  • CDC7/Dbf4 kinase activity is required for initiation of DNA replication and subsequent transition into S- phase of the cell cycle.
  • Drfl A second activator protein of CDC7 called Drfl or ASKLl has been identified in human cells, and appears to be involved in both S and M phase progression (Montagnoli et al., "Drfl, a novel regulatory subunit for human CDC7 kinase” EMBO J 21(12):3171-81 (2002); Yoshizawa-Sugata, "A second human Dbf4/ASK-related protein, Drfl /ASKLl, is required for efficient progression of S and M phases" Biol Chem 280(13): 13062-70 (2005)).
  • CDC7 knock-out mice are embryonic lethal between E3.5 and E6.5 (Kim et al., "Inactivation of CDC7 kinase in mouse ES cells results in S-phase arrest and p53-dependent cell death” EMBO J 21(9):2168-79 (2002)).
  • conditional CDC7 as well as conditional Dbf4 knock-out ES cell lines revealed the essential roles of both proteins in mammalian cell proliferation and DNA synthesis (Kim et al., "Hypomorphic mutation in an essential cell-cycle kinase causes growth retardation and impaired spermatogenesis" EMBO J 22(19):5260-72 (2003); Yamashita et al, "Functional analyses of mouse ASK, an activation subunit for CDC7 kinase, using conditional ASK knockout ES cells” Genes Cells 10(6):551-63 (2005)).
  • CDC7 has emerged as an attractive target for cancer therapy. Depletion of CDC7 using siRNA oligonucleotides results in induction of apoptosis in cancer cell lines while normal dermal fibroblast cells are spared) Montagnoli et al., Cancer Res 64, 7110 (2004)).
  • CDC7 mediated phosphorylation sites on MCM2, MCM4 and MCM6 in tumor cells have been identified, but the functional relevance of those sites remains to be determined (Montagnoli et al., J of Biol Chem 281 :10281 (2006); Tsuji et al., MoI Biol Cell 17:4459-4472 (2006); Masai et al., J Biol Chem 281 :39249-39261 (2006); Sheu et al., MoI Cell 24:101-113 (2006)).
  • the CDC7/Dbf4 complex is a target of the S checkpoint response to genotoxic stress. In HU-treated S.
  • This disclosure relates to compounds and pharmaceutical compositions of the compounds for inhibiting protein kinases such as PIM (PIM-I, PIM-2 and/or PIM- 3), CDC7 or CK2.
  • PIM protein kinases
  • PIM-I, PIM-2 and/or PIM- 3 protein kinases
  • CDC7 protein kinases
  • CK2 protein kinases
  • Another aspect of this dislcosure relates to a pharmaceutical composition, comprising a compound according to Formula I and a pharmaceutically acceptable carrier, excipient, or diluent.
  • Another aspect of this dislcosure relates to a method of inhibiting PIM, CDC7 or CK2 in a cell, comprising contacting the cell, in which inhibition of PIM, CDC7 or CK2 is desired, with a compound according to Formula I.
  • Another aspect of this dislcosure relates to a method of inhibiting PIM, CDC7 or CK2 in a cell, comprising contacting a cell in which inhibition of PIM is desired with a pharmaceutical composition comprising a compound according to Formula I and a pharmaceutically acceptable carrier, excipient, or diluent.
  • Another aspect of this dislcosure relates to a method of treating a disease or condition that involves PIM, CDC7 or CK2, comprising administering to a patient, in need of said treatment, a compound according to Formula I.
  • Another aspect of this dislcosure relates to a method of treating a disease or condition that involves PIM, CDC7 or CK2, comprising administering to a patient, in need of said treatment, a pharmaceutical composition comprising a compound according to Formula I and a pharmaceutically acceptable carrier, excipient, or diluent.
  • a pharmaceutical composition comprising a compound according to Formula I and a pharmaceutically acceptable carrier, excipient, or diluent.
  • the disease or condition that can be treated by the compounds of Formula I, and the pharmaceutical compositions thereof, include cancer.
  • Non- limiting examples of the types of cancer that can be treated include ovarian cancer, pancreatic cancer, prostate cancer, hepatocellular carcinoma, lymphomas, leukemias, cervical cancer, breast cancer (including breast carcinoma), colorectal cancer (including colorectal carcinoma), malignant melanoma, non-small cell lung cancer (NSCL) or glioblastomas.
  • the disease or condition that can be treated by the compound of formula I includes pancreatic cancer, prostate cancer, hepatocellular carcinoma, lymphomas, leukemias, colorectal cancer, breast carcinoma, colorectal carcinoma, malignant melanoma, and non-small cell lung cancer.
  • the disease or condition that can be treated by the compound of formula I includes breast carcinoma, colorectal carcinoma, malignant melanoma, and non- small cell lung cancer (NSCL).
  • NSC non- small cell lung cancer
  • R 1 is hydrogen or alkyl
  • R 2 is selected from aminocarbonylalkylaminoalkyl, aminoalkylaminoalkyl, dialkylaminoalkylaminoalkyl, carboxyalkylaminoalkyl, cycloakylaminoalkyl, dialkylaminoarylalkylaminoalkyl, heteroarylalkylaminoalkyl, arylalkyl optionally substituted at any aryl or alkyl position with 1-3 groups selected from halo and -NH 2 , heterocycloalkylarylalkylaminoalkyl optionally substituted at the heterocycloalkyl portion with alkyl, aminoalkyl optionally substituted with 1 , 2 or 3 -OH, alkylamino optionally substituted with 1 , 2 or 3 -OH, alkylaminoalkyl optionally substituted with 1, 2 or 3 -OH, alkoxyalkylaminoalkyl, heterocycloalkylaminoalkyl optionally substituted with alkyl at
  • R 3a is selected from halo, alkyl, -NO 2 , alkoxy, alkynyl optionally substituted with R 14 , alkoxycarbonylalkyl, arylalkoxy, -C(O)N(H)alkyl, -N(H)-C(O)-alkyl, - C(O)-alkyl,
  • R 3b , R 3c and R 3d are each independently selected from H, -OH, -N + (O)OH, alkoxyl, and halo; or R 3a is hydrogen and R 3b , R 3c and R 3d are each independently selected from -CF 3 , -OH, alkoxy, and halo; or R 3a and R 3d , together with the carbons to which they are attached, join to form a 5 membered heteroaryl optionally substituted with methyl or -NH 2 , or a 5-6 membered heterocycloalkyl;
  • R 4 is selected from -OH, amino, aminoalkyl, halo, alkyl optionally substituted with -OH, alkoxy, alkylaminoalkyl, heteroarylalkyl, -C(O)OH, -C(O)-O-alkyl, -C(O)- alkyl, oxo, aryl optionally substituted with alkyl, arylalkyl or halo, heteroaryl, -OH, dialkylamino, dialkylaminoalkyl, alkylamino, spiro-heterocycloalkyl, -NHC(O)R 8 , -C(O)NHR 9 , arylalkylaminocarbonyl optionally substituted with halo at any ring position of the aryl, heterocycloalkylalkylamino, dialkylaminoalkylcarbonyl, dialkylaminocarbonylalkyl, heterocycloalkylalkyl optionally
  • R 5 is selected from alkyl, -OH, amino, aminoalkyl, -C(O)N(H)- heteroarylalkyl, halo, -NO 2 , -C(O)-N(H)-heterocycloalkylalkyl, alkylaminoalkyl, heteroaryl, cycloalkylaminoalkyl, alkylamino, dialkylamino, -C(O)Oalkyl, -C(O)OH, heterocycloalkyl, -N(H)-alkylheterocycloalkylC(O)-O-alkyl, -O-alkyl-C(O)-N(H)- alkylcycloalkyl, -C(O)-N(H)-alkyl, -C(O)N(H)alkyla
  • R 6 is selected from dialkylaminoalkyl, heteroarylamino, heterocycloalkyl, heterocycloalkylalkyl optionally substituted with -OH, cycloalkyl, heteroarylalkyl, alkoxyalkyl, heterocycloalkyl, heteroaryl optionally substituted with 1 , 2 or 3 groups selected from halo, -NH 2 , aminoalkylaminocarbonyl, heteroaryl, hydroxyalkyl, alkoxy, alkyl, -C(O)-O-alkyl and -C(O)-O-H, alkyl, alkoxy, and aryl optionally substituted 1, 2 or 3 halo, -N(H)C(O)CH 3 , alkyl or alkoxy;
  • R 7 is selected from heterocycloalkylalkyl, arylalkyl optionally substituted at any ring position with 1, 2 or 3 halo groups, dialkylaminoalkyl, heterocycloalkyl, heteroaryl optionally substituted at any ring position with 1 , 2 or 3 groups selected from halo and -COOH, and alkoxyalkyl;
  • R 8 is selected from arylalkyl, heterocycloalkyl and alkyl
  • R 9 is selected from H, alkyl, arylalkyl optionally substituted with halo at any ring position of the aryl, heterocycloalkyl, arylalkylaminocarbonyl optionally substituted with halo and dialkylaminoalkyl;
  • R 10 is selected from alkyl, oxo, heteroaryl, dialkylaminoalkylcarbonyl, dialkylaminocarbonylalkyl, aminoalkyl, -OH, halo, heteroarylcarbonyl, dialkylaminoalkyl, heterocycloalkyl optionally substituted with alkyl,
  • arylalkylcarbonyl arylcarbonyl, alkylcarbonyl, alkoxyalkylcarbonyl, heterocycloalkylcarbonyl, heteroarylalkyl, -O-heterocycloalkyl and arylalkyl optionally substituted with alkoxy or arylalkoxy;
  • R 11 is selected from aryl optionally substituted with halo, heteroarylalkyl, cycloalkyl, spiro-cycloalkyl and arylalkyl optionally substituted with alkoxy or phenylmethylmethoxy;
  • R 12 is selected from -NH 2 and heterocycloalkyl optionally substituted with alkyl;
  • R 13 is selected from arylalkyl wherein the aryl portion of arylalkyl is optionally substituted with 1, 2 or 3 alkoxy, halo, methyl, methoxy, -CF 3 , cycloalkyl (such as cyclohexyl), and heteroarylalkyl (such as pyridinealkyl); and
  • R 14 is selected from hydroxylalkyl, H and TMS.
  • R 3a is selected from halo, alkyl, - NO 2 , alkoxy, alkynyl optionally substituted with R 14 , alkoxycarbonylalkyl, arylalkoxy,
  • R 3b , R 3c and R 3d are each H. [0022] In another embodiment of formula I, R 3a is halo, alkoxy or -OCF 3; and
  • R 3b , R 3c and R 3d are each H.
  • Aspect (B) of this disclosure relates to a compound of Formula I,
  • R 1 is hydrogen or alkyl
  • R 2 is selected from aminocarbonylalkylaminoalkyl, aminoalkylaminoalkyl, dialkylaminoalkylaminoalkyl, carboxyalkylaminoalkyl, cycloakylaminoalkyl, dialkylaminoarylalkylaminoalkyl, heteroarylalkylaminoalkyl, arylalkyl, heterocycloalkylarylalkylaminoalkyl optionally substituted at the heterocycloalkyl portion with alkyl, alkoxyalkylaminoalkyl, heterocycloalkylaminoalkyl optionally substituted with alkyl at the heterocycloalkyl portion, hydroxyalkyl, cycloalkylaminoalkyl, arylamino(alkyl)alkyl optionally substituted at any ring position with 1, 2 or 3 halo, heterocycloalkylalkylamino
  • R 3b , R 3c and R 3d are each independently selected from H, -OH, -N + (O)OH, alkoxyl, and halo; or R 3a is hydrogen and R 3b , R 3c and R 3d are each independently selected from -CF 3 , -OH, alkoxy, and halo; or R 3a and R 3d , together with the carbons to which they are attached, join to form a 5 membered heteroaryl optionally substituted with methyl or -NH 2 , or a 5-6 membered heterocycloalkyl;
  • R 4 is selected from -OH, amino, aminoalkyl, halo, alkyl optionally substituted with -OH, alkoxy, alkylaminoalkyl, heteroarylalkyl, -C(O)OH, -C(O)-O-alkyl, -C(O)- alkyl, oxo, aryl optionally substituted with alkyl or halo, heteroaryl, -OH, dialkylamino, dialkylaminoalkyl, alkylamino, spiro-heterocycloalkyl, -NHC(O)R 8 , -C(O)NHR 9 , arylalkylaminocarbonyl optionally substituted with halo at any ring position of the aryl, heterocycloalkylalkylamino, dialkylaminoalkylcarbonyl, dialkylaminocarbonylalkyl, heterocycloalkylalkyl optionally substituted with -CF 3
  • R 5 is selected from -C(O)N(H)-heteroarylalkyl, -C(O)-N(H)- heterocycloalkylalkyl, alkylaminoalkyl, cycloalkylaminoalkyl, -N(H)-alkylheterocycloalkylC(O)-O-alkyl, -O-alkyl-C(O)-N(H)-alkylcycloalkyl, -C(O)N(H)alkylaryl, -C(O)N(H)-cycloalkyl, -O-alkylheterocycloalkyl, heteroarylalkylamino, heterocycloalkylalkylamino optionally substituted with alkyl at any ring position, alkoxycarbonylheterocycloalkylaminoalkyl, heterocycloalkylaminoalkyl optionally substituted at the heterocycloalkyl portion with alkoxy
  • R 6 is selected from dialkylaminoalkyl, heteroarylamino, heterocycloalkyl, heterocycloalkylalkyl optionally substituted with -OH, cycloalkyl, heterocycloalkylalkyl, heteroarylalkyl, alkoxyalkyl, heterocycloalkyl, heteroaryl optionally substituted with 1, 2 or 3 groups selected from halo, -NH 2 , aminoalkylaminocarbonyl, heteroaryl, hydroxyalkyl, alkoxy, alkyl, -C(O)-O-alkyl and -C(O)-O-H, alkyl, alkoxy, and aryl optionally substituted 1, 2 or 3 halo, - N(H)C(O)CH 3 , alkyl or alkoxy;
  • R 7 is selected from heterocycloalkylalkyl, arylalkyl optionally substituted at any ring position with 1, 2 or 3 halo groups, dialkylaminoalkyl, heterocycloalkyl, heteroaryl optionally substituted at any ring position with 1, 2 or 3 groups selected from halo and -COOH, and alkoxyalkyl;
  • R 8 is selected from arylalkyl, heterocycloalkyl and alkyl
  • R 9 is selected from H, alkyl, arylalkyl optionally substituted with halo at any ring position of the aryl, heterocycloalkyl, arylalkylaminocarbonyl optionally substituted with halo and dialkylaminoalkyl;
  • R 10 is selected from alkyl, oxo, heteroaryl, dialkylaminoalkylcarbonyl, dialkylaminocarbonylalkyl, aminoalkyl, -OH, halo, heteroarylcarbonyl, dialkylaminoalkyl, heterocycloalkyl (piperidinyl) optionally substituted with alkyl, - C(O)-O-alkyl, arylalkylcarbonyl, arylcarbonyl, alkylcarbonyl, alkoxyalkylcarbonyl, heterocycloalkylcarbonyl, heteroarylalkyl, -O-heterocycloalkyl and arylalkyl;
  • R 11 is selected from aryl optionally substituted with halo, heteroarylalkyl, cycloalkyl, spiro-cycloalkyl and arylalkyl optionally substituted with alkoxy or phenylmethylmethoxy;
  • R 12 is selected from -NH 2 and heterocycloalkyl optionally substituted with alkyl;
  • R 13 is selected from arylalkyl wherein the aryl portion of arylalkyl is optionally substituted with 1, 2 or 3 alkoxy, halo, methyl, methoxy, -CF 3 , cycloalkyl (such as cyclohexyl), and heteroarylalkyl (such as pyridinealkyl); and
  • R 14 is selected from hydroxylalkyl, H and TMS.
  • All of the compounds disclosed herein include either their free base form or their pharmaceutically acceptable salts whether it is stated in the specification that these compounds can exist as their pharmaceutically acceptable salt or not. So, for instance, for any given embodiment of the compound of Formula I (including embodiments relating to the compounds themselves or method of use thereof), this embodiment includes either its free base form or any of its pharmaceutically acceptable salts, whether this is stated within this embodiment or not.
  • R 2 is -CH 2 -Ri S , wherein Ri 5 is selected from aminocarbonylalkylamino, dialkylaminoalkylamino, carboxyalkylamino, cycloakylamino, dialkylaminoarylalkylamino, heteroarylalkylamino, heterocycloalkylarylalkylaminol optionally substituted at the heterocycloalkyl portion with alkyl, amino, alkylamino optionally substituted with 1, 2 or 3 -OH, alkoxy alky lamino, heterocycloalkylamino optionally substituted with alkyl at the heterocycloalkyl portion, cycloalkylamino, arylamino(alkyl) optionally substituted at any ring position with 1, 2 or 3 halo, 4-(4-methylpiperazine-lyl) phenyl] methyloxy, heterocycloalkylalkylamino optionally substituted at any ring position with al
  • R 3a is halo, alkyl, -NO 2 , alkoxy, alkynyl optionally substituted with R 14 , alkoxycarbonylalkyl, arylalkoxy, - C(O)N(H)alkyl,
  • R 3b , R 3c and R 3d are each H.
  • R 3a is halo, alkoxy or -OCF 3; and R 3b , R 3c and R 3d are each H.
  • R 2 in formula I is a heterocycloalkyl selected from azetidinyl, pyrrolidinyl, piperazinyl and piperidinyl optionally substituted with 1, 2 or 3 R 10 .
  • R 2 in formula I is a heterocycloalkyl selected from azetidinyl, pyrrolidinyl, piperazinyl and piperidinyl optionally substituted with 1, 2 or 3 R 10 .
  • R 2 in formula I is a heterocycloalkyl selected from azetidinyl, pyrrolidinyl, piperazinyl and piperidinyl optionally substituted with 1, 2 or 3 R 10 ;
  • R 3a is halo, alkoxy or -OCF 3; and
  • R 3b , R 3c and R 3d are each H.
  • R 2 in formula I is a heteroaryl optionally substituted at any ring position with 1, 2 or 3 substituents selected from amino, alkylamino, halo, -O-heterocycloalkyl, alkoxy, aminoalkyl, dialkylaminoalkylamino, heterocycloalkylalkylamino and heterocycloalkyl.
  • R 2 in formula I is a heteroaryl optionally substituted at any ring position with 1, 2 or 3 substituents selected from amino, alkylamino, halo, -O-heterocycloalkyl, alkoxy, aminoalkyl, dialkylaminoalkylamino, heterocycloalkylalkylamino and heterocycloalkyl;
  • R 3a is halo, alkoxy or -OCF 3; and
  • R 3b , R 3c and R 3d are each H.
  • R 2 in formula I is a heterocycloalkylalkyl optionally substituted with 1 , 2 or 3 R 4 groups at any ring position.
  • R 1 in formula I is H.
  • R 2 in formula I is a heterocycloalkylalkyl optionally substituted with 1, 2 or 3 R 4 groups at any ring position;
  • R 3a is halo, alkoxy or -OCF 3; and
  • R 3b , R 3c and R 3d are each H.
  • R 2 in formula I is an aryl substituted with 1, 2 or 3 R 5 groups at any ring position.
  • R 2 in formula I is an aryl substituted with 1, 2 or 3 R 5 groups at any ring position;
  • R 3a is halo, alkoxy or -OCF 3; and
  • R 3b , R 3c and R 3d are each H.
  • R 2 in formula I is a heterocycloalkyl optionally substituted with 1, 2 or 3 R 10 groups.
  • R 2 in formula I is a heterocycloalkyl optionally substituted with 1, 2 or 3 R 10 groups; R 3a is halo, alkoxy or -OCF 3; and R 3b , R 3c and R 3d are each H.
  • R 2 in formula I is a heteroarylalkyl optionally substituted at any ring position with 1, 2 or 3 alkyl or aryl groups.
  • R 2 in formula I is a heteroarylalkyl optionally substituted at any ring position with 1 2 or 3 alkyl or aryl groups;
  • R 3a is halo, alkoxy or -OCF 3 ; and
  • R 3b , R 3c and R 3d are each H.
  • R 7 in formula I is heteroaryl, such as pyridinyl or imidazolyl, which can be optionally substituted as described above in formula I.
  • R 2 in formula I is heterocycloalkyl, such as morpholinyl, piperidinyl, pyrrolidinyl, piperazinyl or tetrahydrofuan, which can be optionally substituted as described above in formula I.
  • R 2 in formula I is heterocycloalkylaminoalkyl, wherein the heterocycloalkyl potion is piperidinyl, piperazinyl, pyrrolidinyl or imidazolyl, which can be optionally substituted as described above in formula I.
  • R 2 in formula I is heterocycloalkylalkyl, wherein the heterocycloalkyl potion is morpholinyl, piperidinyl, pyrrolidinyl, piperazinyl or tetrahydrofuanyl, which can be optionally substituted as described above in formula I.
  • R 2 in formula I is alkylheterocycloalkyl, wherein the heterocycloalkyl potion is piperizinyl, which can be optionally substituted as described above in formula I.
  • R 2 in formula I is heteroaryl, such as thienyl, furan, pyrazol, thiazol, isoxazol, tetrahydroisoquinolinyl and imidazol, which can be optionally substituted as described above in formula I.
  • R 2 in formula I is phenyl which is substituted as described above in formula I.
  • R 6 is heteroaryl, such as imidazol, oxazole, pyridine, pyrimidine, isoxazole or furanyl, which can be optionally substituted as described above in formula I.
  • R 3a is Br, Cl or -OCH 3
  • R 3b , R 3c and R 3d are each H.
  • R 1 is H.
  • R 2 is heterocycloalkyl, such as piperidinyl, piperazinyl, pyrrolidinyl and morpholinyl, which can be optionally substituted as described above in formula I.
  • R 2 is selected from -(Ci-C 3 )alkyl-phenyl optionally substituted with 1-3 halo, -NH- phenyl,
  • Xa is selected from halo, phenyl substituted with a group selected from - COOH, -COOCH 3 , NO 2 , -(Ci-C 3 )alkoxy, methylthio, -(Ci-C 3 )alkyl, -NH 2 , -OH, -Nt(C 1 - C 3 )alkyl] 2 , -CF 3 and methylsulfonyl;
  • Xb when present, is independently selected from alkyl, -NH 2 and halo;
  • Xc is selected from -(5-6 membered)heteroaryl (imidazole), -(5-6 membered)heterocycloalkyl (piperazinyl), alkylcarbonylamino, alkylaminocarbonyl, cycloalkylaminoalkyl (cyclohexylaminoalkyl), -NH(Ci-C 3 )alkyl-(3-6 membered)heterocycloalkyl, dimethylamino-(Ci-C 3 )alkylcarbonylamino, cycloalkylaminocarbonyl, dialkylaminoalkylcarbonylamino, cycloalkylmethylaminocarbonylmethyloxy, phenylalkylaminocarbonyl, heterocycloalkylaminoalkyl optionally substituted with alkoxycarbonyl, dialkylaminoal
  • -N(H)C (O)phenyl optionally substituted with 1 or 2 groups selected from halo, methyl, methoxy and -NHC(O)CH 3 , -N(H)C(O)alkyl, -N(H)C(O)(C 1 - C 3 )alkylpyridinyl,
  • Xd is selected from alkyl, 1-3 halo, -COOH, phenyl optionally substituted with 1 or 2 groups selected from halo, methyl and methylphenyl, phenylmethyl, spiro- piperidine, trifluoromethylphenylmethyl, -(Ci-C 3 )alkoxy, pyridinyl, dimethylaminoalkyl, dimethylamino, hydroxylalkyl, dimethylaminoalkylaminocarbonyl, alkylamino, aminoalkyl, dimethylaminocarbonylalkyl, diethylaminoalkylcarbonyl, -(Ci-C 3 )alkyl-(5-6 membered)heterocycloalkyl, (5-6 membered)heterocycloalkyl optionally substituted with -(Ci-C 3 )alkyl, -NH 2
  • Xe is selected from dialkylamino, amino, 1-3 -OH, alkoxy, 4- methylpiperazinylphenyl, dimethylaminophenyl, phenyl optionally substituted with 1-
  • Xf is selected from cycloalkyl, spirocycloalkyl, phenyl, phenylalkyl optionally substituted with phenylmethyloxy or alkoxy and thienylalkyl;
  • Xg is selected from alkyl, alkylcarbonyl, heterocycloalkylcarbonyl, dialkylaminoalkylcarbonyl, 1 -methylpiperidinyl, dialkylaminoalkyl, heteroarylcarbonyl, alkoxyalkylcarbonyl, phenylcarbonyl, phenylalkylcarbonyl, oxo, phenylalkyl, -(5-6 membered)heteroarylalkyl, piperidinyloxy, -OH, oxo, 1-2 halo and 1-2 methyl;
  • Rza is H or methyl; and Rzb is H or alkyl optionally substituted with 1-3 -OH.
  • R 2 is selected -NH-phenyl, -NH-piperidinyl, -NH- pyridinyl, -NH(Ci-C 3 )alkylphenyl optionally substituted at any phenyl position with piperazinyl or methylpiperazinyl, -NH(Ci-C 3 )alkyl-N(CH 3 ) 2 , -NH(d-C 3 )alkyl-OH, - (Ci-C 3 )alkyl-O-phenyl, -(Ci-C 3 )alkyl-O-(Ci-C 3 )alkyl-(5-6 membered)heterocycloalkyl, -(Ci-C 3 )alkyl-N(H)-heteroaryl, -(Ci-C 3 )alkyl-(5-
  • Xd is selected from alkyl, 1-3 halo, -COOH, phenyl optionally substituted with 1 or 2 groups selected from halo, methyl and methylphenyl, phenylmethyl, spiro- piperidine, trifluoromethylphenylmethyl, -(Ci-C 3 )alkoxy, pyridinyl, dimethylaminoalkyl, dimethylamino, hydroxylalkyl, dimethylaminoalkylaminocarbonyl, alkylamino, aminoalkyl, dimethylaminocarbonylalkyl, diethylaminoalkylcarbonyl, -(C i -C 3 )alkyl-(5 -6 membered)heterocycloalkyl, (5-6 membered)heterocycloalkyl optionally substituted with -(Ci-C 3 )alkyl, -NH 2 , -OH, heterocycloalkylalkylamino
  • Xe is selected from dialkylamino, amino, 1-3 -OH, alkoxy, A- methylpiperazinylphenyl, dimethylaminophenyl, phenyl optionally substituted with 1- 3 groups selected from halo and methoxy, heteroaryl, -(Ci-C3)alkylC(O)NH 2 , - C(O)NH 2 , -C(O)OH, -(Ci-C 3 )alkylC(O)OH, heterocycloalkyl optionally substituted with 1-2 alkyl;
  • Xf is selected from cycloalkyl, spirocycloalkyl, phenyl, phenylalkyl optionally substituted with phenylmethyloxy or alkoxy and thienylalkyl;
  • Xg is selected from alkyl, alkylcarbonyl, heterocycloalkylcarbonyl, dialkylaminoalkylcarbonyl, 1-methylpiperidinyl, dialkylaminoalkyl, heteroarylcarbonyl, alkoxyalkylcarbonyl, phenylcarbonyl, phenylalkylcarbonyl, oxo, phenylalkyl, -(5-6 membered)heteroarylalkyl, piperidinyloxy, -OH, oxo, 1-2 halo and 1-2 methyl; and
  • Rzb is H or alkyl optionally substituted with 1-3 -OH.
  • R 2 can be any one of the following (22) embodiments for R 2 : [0055] In another embodiment (1), R 2 is -(Ci-C 3 )alkyl-phenyl optionally substituted with 1-3 halo.
  • R 2 is selected from -NH-phenyl, -NH- piperidinyl, -NH-pyridinyl, -NH(Ci-C 3 )alkylphenyl optionally substituted at any phenyl position with piperazinyl or methylpiperazinyl, -NH(Ci-C 3 )alkyl-N(CH 3 ) 2 and -NH(Ci-C 3 )alkyl- OH.
  • R 2 is phenyl substituted with 1-3 Xa groups, wherein Xa is selected from halo, -COOH, -COOCH 3 , NO 2 , -(C r C 3 )alkoxy, methylthio, -(Ci-C 3 )alkyl, -NH 2 , -OH, -N[(Ci-C 3 )alkyl] 2 , -CF 3 and methylsulfonyl.
  • Xa is selected from halo, -COOH, -COOCH 3 , NO 2 , -(C r C 3 )alkoxy, methylthio, -(Ci-C 3 )alkyl, -NH 2 , -OH, -N[(Ci-C 3 )alkyl] 2 , -CF 3 and methylsulfonyl.
  • R 2 is phenyl substituted with 0-2 Xb and 1 Xc group, wherein Xb, when present, is selected from alkyl, -NH 2 and halo, and Xc is selected from -(5-6 membered)heteroaryl (such as, for example, imidazole), -(5-6 membered)heterocycloalkyl (such as, for example, piperazinyl), alkylcarbonylamino, alkylaminocarbonyl, cycloalkylaminoalkyl (such as, for example, cyclohexylaminoalkyl), -NH(Ci-C 3 )alkyl-(3-6 membered)heterocycloalkyl, dimethylamino-(Ci-C 3 )alkylcarbonylamino, cycloalkylaminocarbonyl, dialkylaminoalkylcarbonylamino, cycloalkyl
  • -C(O)OCH 3 -N(H)C(O)imidazolyl optionally substituted with methyl, -N(H)C(O)quinoxalinyl, -N(H)C(O)pyrimidinyl optionally substituted with alkyl, -N(H)C(O)furanyl, -N(H)C(O)pyrazinyl, -N(H)C(O)isoxazolyl optionally substituted with methyl, -N(H)C(O)oxazolyl, or -N(H)C(O)indazolyl optionally substituted with amino), -N(H)C(O)phenyl optionally substituted with 1 or 2 groups selected from halo, methyl, methoxy and -NHC(O)CH 3 , -N(H)C(O)alkyl, -N(H)C(O)(C 1 - C 3 )alkylpyridinyl
  • R 2 is methylpiperazinylphenylalkoxyalkyl (methylpiperazinylphenylmethoxymethyl), methylpiperazinylcarbonyl, 2- chlorophenyl-4-methylpiperazinylmethyl, (4-methylpiperazin- 1 -yl)(phenyl)methyl, 1 - (4-methylpiperazin- 1 -yl)-2-phenylethyl, or 2-chlorophenyl(4-methylpiperazin- 1 - yl)methyl, 4-oxo-3-phenyl-l,3,8-triazaspiro[4.5]dec-l-yl)methyl.
  • R 2 is -(Ci-C 3 )alkylC(O)OH.
  • R 2 is hydroxyalkyl.
  • R 2 is -(Ci-C 3 )alkyl-N(Rz)-aryl (wherein aryl can be, for example, phenyl or 2,3-dihydro-lH-indenyl) optionally substituted with chloro, fluoro, piperazinyl, methylpiperazinyl or dialkylamino(dimethylamino), and Rz is H or methyl.
  • aryl can be, for example, phenyl or 2,3-dihydro-lH-indenyl
  • Rz is H or methyl.
  • R 2 is -(5-10)membered heteroaryl (such as, for example, thienyl, pyridinyl, indazolyl, imidazolyl, thiazolyl, isoxazolyl, pyrazolyl, pyrimidinyl, benzimidazoly, pyrazolo[l,5-a]pyrimidinyl, tetrahydroisoquinolinyl, or 5,6,7,8-tetrahydroimidazo[l,2-a]pyrazinyl), optionally substituted with 1 or 2 groups selected from halo, -(Ci-Ce)alkyl, -(Ci-Ce)alkoxy, piperidinylalkylamino, piperdinylalkylamino, alkylamino, aminoalkyl, dialkylaminoalkylamino, piperidinyloxy, piperidinyl, and amino.
  • heteroaryl such as, for example, thien
  • Non- limiting examples of this embodiment include 5-chloro-2-thienyl, thienyl, 3 -methyl- lH-indazo IyI, 3 -methyl- lH-indazo IyI, 3- [(2-methylpropyl)oxy]pyridin-4-yl, 3-amino-lH-indazolyl, 3 -amino-5 -chloro- IH- indazolyl, lH-benzimidazolyl, lH-imidazolyl, 1,3-thiazolyl, 2-amino-5- chloropyrimidin-4-yl, 3-[(piperidin-4-ylmethyl)amino]-lH-indazolyl, and (2S)-2,3- dihydro- 1 H-indol-2-yl.
  • R 2 is -(C 1 -C 3 )alkyl-O-phenyl.
  • R 2 is -(Ci-C 3 )alkyl-O-(Ci-C 3 )alkyl-(5-6 membered)heterocycloalkyl, wherein the heterocycloalkyl can be, for example, pyrrolidinyl.
  • R 2 is -(Ci-C 3 )alkyl-N(H)-heteroaryl wherein the heteroaryl can be, for example, pyridinyl.
  • R 2 is -(Ci-C 3 )alkyl-(5-10)membered heteroaryl (wherein the heteroaryl can be, for example, imidazolyl or pyrazolyl) optionally substituted with -(Ci-C 3 )alkyl, halo and phenyl.
  • R 2 is oxopyrrolidinyl optionally substituted with OH and/or piperidinyl.
  • R 2 is -(Ci-C 4 )alkyl-(3-9 membered)heterocycloalkyl optionally substituted at any position of the (3-9 membered)heterocycloalkyl with Xd, wherein Xd is selected from alkyl( such as, for example, methyl), 1-3 halo, -COOH, phenyl optionally substituted with 1 or 2 groups selected from halo, methyl and methylphenyl, phenylmethyl, spiro-piperidine, trifluoromethylphenylmethyl, -(Ci-C 3 )alkoxy, pyridinyl, dimethylaminoalkyl (such as, for example, dimethylamino-(Ci-C 3 )alkyl), dimethylamino, hydroxylalkyl, dimethylaminoalkylaminocarbonyl (such as, for example, dimethylamino(Ci-
  • alkylaminocarbonyl alkylamino, aminoalkyl (such as, for example, aminomethyl or aminoethyl), dimethylamino carbonylalkyl (such as, for example, dimethylaminocarbonylmethyl), diethylaminoalkylcarbonyl (such as, for example, diethylaminomethylcarbonyl), -(Ci-C 3 )alkyl-(5-6 membered)heterocycloalkyl, (5-6 membered)heterocycloalkyl (such as, for example, piperidinyl or morpholinyl) optionally substituted with -(Ci-C 3 )alkyl, -NH 2 , -OH, heterocycloalkylalkylamino, alkoxyalkyl (such as for example, methoxy ethyl), -C(O)CH 3 , -C(O)NH(Ci- C 3 )alkylphenyl optionally
  • the (3-9 membered)heterocycloalkyl can be, for example, piperazinyl, piperidinyl, pyrrolidinyl, azetidinyl, morpholinyl, 1 ,4-diazepanyl, 2,5-diazabicyclo[2.2.1]heptyl, azabicyclo[2.2.1]heptane such as anti-7-hydroxy-2-azabicyclo[2.2.1]heptane, 7- hydroxy-2-azabicyclo[2.2.1]heptanyl and (7S)-7-hydroxy-2- azabicyclo[2.2.1]heptanyl, 8-azabicylo[3.2.1]oct-8-yl such as 3-hydroxy-8- azabicylo[3.2.1]oct-8-yl, (lS,4S)-2,5-diazabicyclo[2.2.1]heptyl such as (lS,4S)-5- methyl-2,5-diazabicyl, (
  • the -(Ci-C4)alkyl-(3-9 membered)heterocycloalkyl described above is -CH 2 -(5-7 membered)heterocycloalkyl, which can be optionally substituted with any of the optional substituents described above for -(Ci-C4)alkyl-(3-9 membered)heterocycloalkyl.
  • R 2 is -(CH 2 )-(5-6 membered)heterocycloalkyl optionally substituted with any of the optional substituents described above for -(Ci-C4)alkyl-(3-9 membered)heterocycloalkyl.
  • Xd is bonded to the -(3-9 membered)heterocycloalkyl portion of the -(Ci-C 4 )alkyl-(3-9 membered)heterocycloalkyl in the (S) stereochemical configuration. In another embodiment, Xd is bonded to the -(3-9 membered)heterocycloalkyl portion of the -(Ci-C4)alkyl-(3-9 membered)heterocycloalkyl in the (R) stereochemical configuration.
  • R 2 is -(C 1 -C 6 )alkyl-NRz-(C 1 -C 4 )alkyl wherein the -(Ci-C 4 )alkyl portion is optionally substituted with Xe, and Xe is selected from dialkylamino (such as, for example, dimethylamino), amino, 1-3 -OH, alkoxy, A- methylpiperazinylphenyl, dimethylaminophenyl, phenyl optionally substituted with 1- 3 groups selected from halo and methoxy, heteroaryl (such as, for example, furanyl, pyridinyl or imidizolyl), -(Ci-C 3 )alkylC(O)NH 2 , -C(O)NH 2 , -C(O)OH, -(C 1 -
  • C 3 )alkylC(O)OH C 3 )alkylC(O)OH, heterocycloalkyl (such as, for example, morpholinyl, pyrrolidinyl piperidinyl or piperazinyl) optionally substituted with 1-2 alkyl (non-limiting examples include l,l-dimethyl-2 -pyrrolidinyl, l,l-dimethyl-2-piperidinyl, and 4- methylpiperazinyl), wherein Rz is H or alkyl optionally substituted with 1-3 -OH.
  • heterocycloalkyl such as, for example, morpholinyl, pyrrolidinyl piperidinyl or piperazinyl
  • Rz is H or alkyl optionally substituted with 1-3 -OH.
  • the -(C 1 -C 6 )alkyl-NRz-(C 1 -C 4 )alkyl above is -(C 1 -C 3 )alkyl- NRz-(C 1 -C 4 )alkyl which can be optionally substituted with any of the optional substituents described above for -(C 1 -C6)alkyl-NRz-(C 1 -C 4 )alkyl.
  • R 2 is -(Ci-C 3 )alkyl-NH-(C 3 -C 6 )cycloalkyl such as, for example, -(CH 2 )-NH-(C 3 -C 6 )cyclohexyl.
  • R 2 is -(Ci-C 3 )alkyl-NH 2 , wherein the -(C 1 - C 3 )alkyl- potion is optionally substituted with Xf, and Xf is selected from cycloalkyl (such as, for example, cyclohexyl), spirocycloalkyl, phenyl, phenylalkyl optionally substituted with phenylmethyloxy or alkoxy and thienylalkyl.
  • cycloalkyl such as, for example, cyclohexyl
  • spirocycloalkyl such as, for example, cyclohexyl
  • spirocycloalkyl such as, for example, cyclohexyl
  • spirocycloalkyl such as, for example, cyclohexyl
  • spirocycloalkyl such as, for example, cyclohexyl
  • spirocycloalkyl
  • Non-limiting examples of this embodiment include any one or more of the following groups: -(CH 2 )S-NH 2 , - CH 2 -NH 2 , -(CH 2 ) 2 -NH 2 , -CH 2 -CH(CH 3 )-NH 2 , or -C(CH 3 ) 2 -NH 2.
  • R 2 is (3-9 membered)heterocycloalkyl (such as, for example, piperazinyl, piperidinyl, pyrrolidinyl, isoxazolyl, azetidinyl, morpholinyl, tetrahydrofuranyl, thiazolidinyl or octahydro-lH-indolyl) optionally substituted with Xg, and Xg is selected from alkyl (such as, for example (C 1 - C 3 )alkyl), alkylcarbonyl (such as, for example, -C(O)CH 3 ), heterocycloalkylcarbonyl, dialkylaminoalkylcarbonyl (such as, for example, 4- (dimethylamino)butanoyl, 4-(dimethylamino)propanoyl or 2- (dimethylamino)ethanoyl) 1 -methylpiperidinyl, dialkylaminoalkylcarbon
  • R 2 is (3-9 membered)heterocycloalkyl
  • R 2 is bonded to the parent moiety in the (S) stereochemical configuration.
  • R 2 is (3-9 membered)heterocycloalkyl
  • R 2 is bonded to the parent moiety in the (R) stereochemical configuration.
  • R 2 is (5 membered)heterocycloalkyl optionally substituted with methyl.
  • R 2 is (5 membered)heterocycloalkyl optionally substituted with halo.
  • R 2 is (5 membered)heterocycloalkyl optionally substituted with-OH.
  • R 2 is (5 membered)heterocycloalkyl optionally substituted with phenylmethyl. In another embodiment, R 2 is (5 membered)heterocycloalkyl optionally substituted with -C(O)CH 3 . In another embodiment, R 2 is (5 membered)heterocycloalkyl optionally substituted with dialkylaminoalkylcarbonyl. In another embodiment, R 2 is (5 membered)heterocycloalkyl optionally substituted with dialkylaminoalkyl heteroarylcarbonyl. In another embodiment, R 2 is (6 membered)heterocycloalkyl optionally substituted with methyl.
  • R 2 is (6 membered)heterocycloalkyl optionally substituted with halo. In another embodiment, R 2 is (6 membered)heterocycloalkyl optionally substituted with-OH. In another embodiment, R 2 is (6 membered)heterocycloalkyl optionally substituted with phenylmethyl. In another embodiment, R 2 is (6 membered)heterocycloalkyl optionally substituted with -C(O)CH 3 . In another embodiment, R 2 is (6 membered)heterocycloalkyl optionally substituted with dialkylaminoalkylcarbonyl. In another embodiment, R 2 is (6 membered)heterocycloalkyl optionally substituted with dialkylaminoalkyl heteroarylcarbonyl.
  • R 2 is -(C 3 -C 6 )-cycloalkyl (cyclohexyl) optionally substituted with amino, -NHC(O)-O-(CH 3 ) 3 or aminoalkyl (such as, for example, aminomethyl).
  • R 2 is dialkylaminoalkylamino, such as, for example 3-(dimethylamino)propylamino.
  • R 2 is
  • Ri 5 is selected from H or -(Ci -C 6 )alkyl
  • Ri 6 is selected from H, phenyl and -(Ci-C 6 )alkyl
  • R n is selected from H, -(Ci-C 3 )alkylC(O)NH 2 , -(Ci-C 3 )alkylC(O)OH and heterocycloalkylalkyl (such as, for example, 1,1-dimethyl- 2-pyrrolidin- 1 -ylethyl or 1 , 1 -dimethy 1-2-piperidin- 1 -ylethyl) .
  • All compounds of formula I for each of Aspect A (and Aspect B as applicable) disclosed above include any of the disclosed alternative aspects or embodiments for each of Ri, R 2 , R3 a , R3b, R3 C , or R3d, in combination with any other of the disclosed alternative aspects or embodiments of R 1 , R 2 , R3 a , R3b, R3c, or R 3 d, as well as any pharmaceutically acceptable salt and stereoisomer of any such combination.
  • any of the alkyl groups referred to in any of the above embodiments, including alkyl portions attached to other groups can be a -(C 1 - Ce)alkyl group.
  • any of the alkyl groups referred to in any of the above embodiments, including alkyl portions attached to other groups, can be a -(C 1 - C 3 )alkyl group.
  • any of the alkoxy groups referred to in any of the above embodiments, including alkoxy portions attached to other groups can be a - (Ci-Ce)alkoxy group.
  • any of the alkoxy groups referred to in any of the above embodiments, including alkoxy portions attached to other groups can be a - (Ci-C 3 )alkoxy group.
  • any of the heterocycloalkyl groups referred to in any of the above embodiments, including heterocycloalkyl portions attached to other groups, can be a (4-6 membered) heterocycloalkyl group.
  • any of the cycloalkyl groups referred to in any of the above embodiments can be a -(C3-Ce)cycloalkyl group.
  • Activity A in Table 1 is meant to mean the compound has a CDC7 IC 50 value of less than 10,000 nanomolar (nm).
  • Activity B in Table 1 is meant to mean the compound has a PIM IC50 value of less than 10,000 nanomolar (nm).
  • Activity C in Table 1 is meant to mean the compound has a CK2 IC50 value of less than 10,000 nanomolar (nm).
  • Another aspect of this dislcosure relates to a pharmaceutical composition
  • a pharmaceutical composition comprising a compound according to Formula I, or a pharmaceutically acceptable salt thereof, and a pharmaceutically acceptable carrier, excipient, or diluent.
  • Another aspect of this dislcosure relates to a method of inhibiting PIM, CDC7 or CK2 in a cell, comprising contacting a cell in which inhibition of PIM, CDC7 or CK2 is desired with a compound according to Formula I, or a pharmaceutically acceptable salt thereof.
  • Another aspect of this dislcosure relates to a method of inhibiting PIM, CDC7 or CK2 in a cell, comprising contacting a cell in which inhibition of PIM, CDC7 or CK2 is desired with a pharmaceutical composition comprising a compound according to Formula I, or a pharmaceutically acceptable salt thereof, and a pharmaceutically acceptable carrier, excipient, or diluent.
  • Another aspect of this dislcosure relates to a method of treating a disease or condition, including those that involve PIM, CDC7 or CK2, comprising administering to a patient, in need of the treatment, a compound according to Formula I, or a pharmaceutically acceptable salt thereof.
  • Non- limiting examples of the disease or condition that can be treated include cancer such as ovarian cancer, pancreatic cancer, prostate cancer, hepatocellular carcinoma, lymphomas, leukemias, cervical cancer, breast cancer, colorectal cancer, non-small cell lung cancer (NSCL) or glioblastomas.
  • the disease or condition that can be treated is selected from pancreatic cancer, prostate cancer, hepatocellular carcinoma, lymphomas, leukemias, colorectal cancer and non-small cell lung cancer.
  • the disease or condition that can be treated is selected from pancreatic cancer, prostate cancer, hepatocellular carcinoma, lymphomas and leukemias.
  • the disease or condition that can be treated is selected from colorectal cancer and non-small cell lung cancer.
  • Another aspect of this disclosure relates to a compound in Table 1 that has a CDC7 IC50 value of less than 3000 nm, or a pharmaceutically acceptable salt of such a compound.
  • the compounds in Table 1 that have a CDC7 IC50 value of less than 3000 nm are compounds 1-3, 5, 13, 24, 28, 32-35, 40, 43, 45, 48, 49, 52, 60, 64, 100, 103, 107, 108-109, 111-112, 119, 121, 127-128, 130, 138, 140-144, 147-148, 153- 155, 157-161, 164, 166-167, 169, 173-175, 178, 181, 182-186, 189, 191, 194-195, 197-199, 201, 205, 214, 216-217, 220-221, 223-224, 226, 231-232, 235, 248, 252, 259, 262, 264, 266, 269, 270, 273, 277, 283, 287, 289-290, 298, 302, 305, 307, 308, 311, 313, 315, 317, 319, 323, 324, 330-333, 344, 346, 348, 349
  • Another aspect of this disclosure relates to a compound in Table 1 that has a CDC7 IC50 value of less than 100 nm, or a pharmaceutically acceptable salt of such a compound.
  • the compounds in Table 1 that have a CDC7 IC50 value of less than 100 nm are compounds 1-2, 5, 28, 49, 103, 108, 119, 128, 147, 157-158, 164, 166- 167, 169, 173-175, 183-186, 191, 198-199, 201, 214, 216, 221, 223, 231-232, 242, 252, 259, 262, 269, 270, 273, 277, 287, 289, 298, 307, 308, 317, 331-333, 344, 346, 348, 364, 366, 368-369, 375, 379-380, 383, 400-402, 404, 407-408, 412-413, 421, 426, 429, 432, 437, 442, 452, 453, 458, 470-471
  • Another aspect of this dislcosure relates to a method of treating a disease or condition, including those that involve PIM, CDC7 or CK2, comprising administering to a patient, in need of the treatment, a pharmaceutical composition comprising a compound according to Formula I, or a pharmaceutically acceptable salt thereof, and a pharmaceutically acceptable carrier, excipient, or diluent.
  • a pharmaceutical composition comprising a compound according to Formula I, or a pharmaceutically acceptable salt thereof, and a pharmaceutically acceptable carrier, excipient, or diluent.
  • the disease or condition that can be treated include cancer such as ovarian cancer, pancreatic cancer, prostate cancer, hepatocellular carcinoma, lymphomas, leukemias, cervical cancer, breast cancer, colorectal cancer, non-small cell lung cancer (NSCL) or glioblastomas.
  • NSC non-small cell lung cancer
  • the disease or condition that can be treated is selected from pancreatic cancer, prostate cancer, hepatocellular carcinoma, lymphomas, leukemias, colorectal cancer and non-small cell lung cancer. In another embodiment, the disease or condition that can be treated is selected from pancreatic cancer, prostate cancer, hepatocellular carcinoma, lymphomas and leukemias. In another embodiment, the disease or condition that can be treated is selected from colorectal cancer and non-small cell lung cancer.
  • Another aspect of this dislcosure relates to a method of treating a disease or condition, including those that involve PIM, CDC7 or CK2 comprising administering to a patient, in need of the treatment, a compound according to Formula I, or a pharmaceutically acceptable salt thereof, in combination with radiation treatment and/or one or more therapeutic angents selected from Camptothecin, Topotecan, 9-Nitrocamptothecin, 9-Aminocamptothecin, Karenitecin, Irinotecan, Etoposide, Etoposide Phosphate, Teniposide, Amsacrine, Razoxane, Dexrazoxane, Mechlorethamine, Cyclophosphamide, Ifosfamide, Chlorambucil, Melphalan, Thiotepa, Trenimon, Triethylenemelamine, Rapamycin, Dianhydrogalactitol, Dibromodulcitol, Busulfan, dimethylsulfate,
  • a substituent "R” can reside on any atom of the ring system, assuming replacement of a depicted, implied, or expressly defined hydrogen from one of the ring atoms, so long as a stable structure is formed. [0097] If a group "R” is depicted as floating on a fused ring system, as for example in the formulae:
  • the "R” group can reside on either the 5-membered or the 6-membered ring of the fused ring system.
  • the two "R's" can reside on any two atoms of the ring system, again assuming each replaces a depicted, implied, or expressly defined hydrogen on the ring.
  • administering means introducing the compound or a prodrug of the compound into the system of the animal in need of treatment.
  • administering When a compound of this disclosure or prodrug thereof is provided in combination with one or more other active agents (e.g., surgery, radiation, and chemotherapy, etc.), "administration" and its variants are each understood to include concurrent and sequential introduction of the compound or prodrug thereof and other agents.
  • active agents e.g., surgery, radiation, and chemotherapy, etc.
  • Alkyl is intended to include molecules having 1-12 carbons in size (C 1 - Ci2)alkyl, which can be straight chained or branched.
  • C 6 alkyl can refer to an n-hexyl, ⁇ o-hexyl, cyclobutylethyl, and the like.
  • Alkyl is intended to include lower alkyl groups of from 1-6 carbons in size, such as methyl, ethyl, propyl, isopropyl, butyl, s-butyl, t-butyl, isobutyl, pentyl, hexyl and the like.
  • Higher alkyl refers to alkyl groups containing more that six carbon atoms.
  • alkyl residue having a specific number of carbons is named, all geometric isomers having that number of carbons are intended to be encompassed; thus, for example, either "butyl” or “C 4 alkyl” is meant to include n-butyl, sec -butyl, isobutyl, t-butyl, and for example,
  • propyl or "C3 alkyl” each include n-propyl and isopropyl.
  • -(Ci-Ce)alkyl is a subset of alkyl groups that are from one to six carbon atoms in length, and can be straight chained or branched.
  • -(Ci-C 3 )alkyl is a subset of alkyl groups that are from one to three carbon atoms in length, and can be straight chained or branched.
  • alkenyl is intended to be an alkyl that contains at least one double bond between two carbons.
  • Non-limiting examplels of alkenyl include vinyl, allyl, isoprenyl, and the like.
  • alkynyl is intended to be an alkyl that contains at least one triple bond between two carbons.
  • Cycloalkyl means a non-aromatic mono- or multicyclic ring system comprising about 3 to about 14 carbon atoms.
  • Non-limiting examples of monocyclic cycloalkyls include cyclopropyl, cyclopentyl, cyclohexyl, cycloheptyl and the like.
  • Non- limiting examples of multicyclic cycloalkyls include 1-decalin, norbornyl, adamantyl and the like. Cycloalkyls can be fused or bridge ring systems or spirocyclic systems.
  • "-(C 3 -C 6 )cycloalkyl” is a subset of cycloalkyl and means a non-aromatic monocyclic ring system comprising from 3 to 6 carbon atoms.
  • Alkoxy or "alkoxyl” both refer to the group -O-alkyl, wherein the “alkyl” portion is as defined hereinabove. Examples include methoxy, ethoxy, propoxy, isopropoxy, and the like.
  • Aryl means a monovalent six- to fourteen-membered mono- or multicyclic ring, wherein the monocyclic ring is aromatic and at least one of the rings in the multicyclic ring is aromatic.
  • a multicyclic ring that contains only one aryl ring is intended to be included within the definition of aryl.
  • An aryl can also be six- to ten membered, or six membered. Representative non-limiting examples of aryl include phenyl, naphthyl, and the like.
  • Arylalkyl means a residue in which an aryl moiety, as defined above, is attached to a parent structure via one of an alkyl (i.e, alkylene, alkenylene, or alkynylene), wherein the "aryl” and “alkyl” portions are as defined herein. Examples include benzyl, phenethyl, phenylvinyl, phenylallyl and the like.
  • the "alkyl” portion of the group can be one to ten carbons, and in another embodiment, one to six carbons; the latter can also be referred to as C ⁇ . ⁇ arylalkyl.
  • aryl moiety is attached to a parent structure via an alkylene group.
  • alkylene group examples include benzyl, phenethyl, and the like.
  • two adjacent groups on an aromatic system can be fused together to form a ring structure.
  • the fused ring structure can contain heteroatoms and can be optionally substituted with one or more groups. It should additionally be noted that saturated carbons of such fused groups (i.e. saturated ring structures) can contain two substitution groups.
  • fused-polycyclic or "fused ring system” refers to a polycyclic ring system that contains bridged or fused rings; that is, where two rings have more than one shared atom in their ring structures.
  • fused-polycyclics and fused ring systems includes non-aromatic and aromatic systems.
  • fused-polycyclics share a vicinal set of atoms, for example naphthalene or 1,2,3,4-tetrahydro-naphthalene.
  • a spiro ring system is not a fused-polycyclic by this definition, but fused polycyclic ring systems of the compounds disclosed herein can themselves have spiro rings attached thereto via a single ring atom of the fused- polycyclic.
  • "Halogen” or “halo” both refer to fluorine, chlorine, bromine or iodine.
  • Haloalkyl (which includes alkyl, as defined herein, optionally substituted with up to 8 halo) and "haloaryl” refer generically to alkyl and aryl groups that are substituted with one or more halo, respectively.
  • haloalkyl examples include 3,3,3-trifluoro-l-methylpropyl, 2-methyl-l-(trifluoromethyl)propyl, -CH 2 F, - CHCl 2 and -CF 3 .
  • Heteroatom refers to O, S, N, or P. In another example, the heteroatom is O or N. In another example, the heteroatom is O. In another example, the heteroatom is N.
  • Heterocyclyl refers to a stable three- to f ⁇ fteen-membered ring substituent that consists of carbon atoms and from one to five heteroatoms selected from the group consisting of nitrogen, phosphorus, oxygen and sulfur. For purposes of this disclosure, the heterocyclyl substituent can be a monocyclic, bicyclic or tricyclic ring system, which can include fused or bridged ring systems as well as spirocyclic systems.
  • heterocycloalkyl and “heteroaryl” are groups that are encompassed by the broader term “heterocyclyl.”
  • the nitrogen, phosphorus, carbon or sulfur atoms in the heterocyclyl group can be optionally oxidized to various oxidation states.
  • the group -S(O) 0-2 - refers to -S- (sulfide), - S(O)- (sulfoxide), and -SO 2 - (sulfone) respectively.
  • nitrogens particularly but not exclusively, those defined as annular aromatic nitrogens, are meant to include their corresponding JV-oxide form, although not explicitly defined as such in a particular example.
  • annular nitrogen atoms can be optionally quaternized; and the ring substituent can be partially or fully saturated or aromatic.
  • heterocyclyl groups include, but are not limited to, azetidinyl, acridinyl, benzodioxolyl, benzodioxanyl, benzofuranyl, carbazoyl, cinnolinyl, dioxolanyl, indolizinyl, naphthyridinyl, perhydroazepinyl, phenazinyl, phenothiazinyl, phenoxazinyl, phthalazinyl, pteridinyl, purinyl, quinazolinyl, quinoxalinyl, quinolinyl, isoquinolinyl, tetrazoyl, tetrahydroisoquinolyl, piperidinyl, piperazinyl, 2- oxopiperazinyl, 2-oxopiperidinyl, 2-oxopyrrolidinyl, 2-oxoazepinyl, azepiny
  • Heterocycloalkyl refers to a stable 4-12 membered monocyclic or multicyclic ring, wherein at least one of the rings contain at least one heteroatom and wherein there are no aromatic rings. Heterocycloalkyl is meant to include multicyclic rings wherein one ring contains a heteroatom and another ring does not contain a heteroatom. [00117] "Heterocycloalkylalkyl” refers to a heterocycloalkyl, as defined herein, attached to the parent moiety through an "alkyl,” as defined herein.
  • One non-limiting example of heterocycloalkyl includes piperadinyl. Another non-limiting example of heterocycloalkyl includes piperazinyl. Another non-limiting example of heterocycloalkyl includes furanyl. Another non-limiting example of heterocycloalkyl includes pyrrolidinyl. Another non-limiting example of heterocycloalkyl includes morpholinyl.
  • Amino refers to -NH 2 .
  • Alkylamino refers to -NH(alkyl), wherein “alkyl” portion is as defined above, and wherein the parent moiety is attached to the nitrogen atom.
  • “Dialkylamino” refers to -N(alkyl) 2 , wherein the "alkyl” portiobs are as defined above, and wherein the parent moiety is attached to the nitrogen atom.
  • “Dialkylaminoalkyl” refers to -(alky l)N(alky I) 2 , wherein the "alkyl” portions are as defined above.
  • dialkylaminoalkyl includes -CH 2 C(CH3) 2 CH 2 N(CH3) 2 .
  • aminoalkyl refers to -(alkyl)NH 2 , wherein “alkyl” is as defined above, and wherein the parent moiety is attached to the alkyl group.
  • the amino group can be attached at any point along the alkyl group.
  • Non-limiting examples of aminoalkyl include -CH 2 NH 2 , -CH 2 CH 2 NH 2 , -C(CH 3 ) 2 NH 2 , and -CH(NH 2 )CH 3 .
  • Heteroaryl means a 5- to 12-membered, monocyclic aromatic heterocyclyl (where heterocyclyl is defined herein) or bicyclic heterocyclyl ring system (where at least one of the rings in the bicyclic system is aromatic) where the monocyclic ring and at least one of the rings in the bicyclic ring system contains one, two, three, four, or five heteroatom(s) selected from nitrogen, oxygen, phosphorous, and sulfur.
  • the ring containing the heteroatom can be aromatic or non-aromatic.
  • Representative examples include pyridinyl, imidazolyl, pyrimidinyl, pyrazolyl, triazolyl, pyrazinyl, tetrazolyl, furyl, thienyl, isoxazolyl, thiazolyl, oxazolyl, isothiazolyl, pyrrolyl, quinolinyl, isoquinolinyl, indolyl, benzimidazolyl, benzdioxolyl, benzofuranyl, cinnolinyl, indazolyl, indolizinyl, phthalazinyl, pyridazinyl, triazinyl, isoindolyl, pteridinyl, purinyl, oxadiazolyl, triazolyl, thiadiazolyl, thiadiazolyl, furazanyl, benzofurazanyl, benzothiophenyl, benzothiazolyl
  • heteroaryl groups described herein are selected from thienyl, oxazolyl, furyl, pyrrolyl, imidazolyl, thiazolyl, pyridinyl, imidazolyl and pyrimidinyl.
  • “Carbonyl” refers to the group “-C(O)-", which is bivalent.
  • Aminocarbonyl refers to the group “-C(O)-NH 2 " wherein the parent moiety is attached to the amino group.
  • Alkoxycarbonyl refers to the group “-C(O)alkoxy,” wherein alkoxy is as defined above, and the parent moiety is attached to the carbonyl.
  • a non-limiting example includes -C(O)-OC(CH 3 ) 3 .
  • Hydroxyalkynyl refers to a group wherein the parent moiety is attached to the alkynyl group, as defined above, and a hydroxyl group is attached to the alkynyl.
  • a non- limiting example includes 4-hydroxybut-l-yn-l-yl.
  • “Hydroxyalkyl” refers to a group wherein the paret moiety is attached to the alkyl group, and a hydroxyl group is attached to the alkyl, wherein the alkyl portion is as defined in the term “alkyl” herein
  • alkyl portion is as defined in the term "alkyl” herein.
  • a non- limiting example includes amino(imino)methyl.
  • “Dihydroxyalkyl” refers to a group wherein the parent moiety is attached to the alkyl group, and a two hydroxyl groups are attached to the alkyl, wherein the alkyl portion is as defined in the term “alkyl” herein.
  • “Alkylaminoalkyl” refers to -(alkyl)NH(alkyl), wherein the alkyl portion is as defined in the term “alkyl” herein.
  • Alkylaminoalkylamino refers to -N(H)(alkyl)NH(alkyl), wherein the alkyl portion is as defined in the term “alkyl” herein.
  • Aminoalkylamino refers to -N(H)(alky I)NH 2 , wherein the alkyl portion is as defined in the term “alkyl” herein.
  • Aminoaryalkylamino refers to -N(H)(alkyl)aryl, wherein the "alkyl” and
  • alkylsulfonylheterocycloalkylamino refers to -N(H)-heterocycloalkyl-
  • Cycloalkylalkylamino refers to -N(H)-alkylcycloalkyl, wherein the amino is attached to the parent moiety, and wherein the "alkyl” and “cycloalkyl” portions are as defined herein .
  • Dialkylaminoalkoxy refers to -(alkoxy)N(alkyl) 2 , wherein the "alkoxy” and “alkyl” portions are as defined herein.
  • alkoxy and “alkyl” portions are as defined herein.
  • dialkylaminoalkoxy includes dimethylaminoethyloxy represented by -O-
  • Alkylsulfonylalkylamino refers to -NH 2 -alkyl-S(O) 2 -alkyl, wherein the amino portion of this group is attached to the parent moieity, and wherein the "alkyl" portions are as defined above.
  • a non-limiting example includes methy lsulfony lethy lamino .
  • Aminocarbonylalkylaminoalkyl refers to the group “-alkyl-N(H)-alkyl- C(O)-NH 2 " wherein the parent moiety is attached to the alkyl group, and wherein the alkyl portions are as defined herein.
  • Aminoalkylaminoalkyl refers to the group “-alkyl-N(H)-alkyl-NH 2 " wherein the parent moiety is attached to the alkyl group, and wherein the alkyl portions are as defined herein.
  • Dialkylaminoalkylaminoalkyl refers to the group -alkyl-N(H)-alkyl- N(alkyl) 2 , wherein the parent moiety is attached to the alkyl group, and wherein the
  • alkyl portions are as defined herein. [00142] "Carboxyalkylaminoalkyl” refers to the group -alkyl-N(H)-alkyl-
  • alkyl portions are as defined herein.
  • Cycloakylaminoalkyl refers to the group -alkyl-N(H)-alkyl-cycloalkyl, wherein the parent moiety is attached to the alkyl group, and wherein the "alkyl” and cycloalkyl portions are as defined herein.
  • Dialkylaminoarylalkylaminoalkyl refers to the group -alkyl-N(H)-alkyl- aryl-N(alkyl) 2 , wherein the parent moiety is attached to the alkyl group, and wherein the "alkyl” and “aryl” portions are as defined herein.
  • Heteroarylalkylaminoalkyl refers to the group -alkyl-N(H)-alkyl- heteroaryl, wherein the parent moiety is attached to the alkyl group, and wherein the
  • alkyl and heteroaryl are as defined herein.
  • Heterocycloalkylarylalkylaminoalkyl refers to the group -alkyl-N(H)- alkyl-aryl-heterocycloalkyl, wherein the parent moiety is attached to the alkyl group, and wherein the "alkyl” and “heterocycloalkyl” portions are as defined herein.
  • Alkoxyalkylaminoalkyl refers to the group -alkyl-N(H)-alkyl-O-alkyl, wherein the parent moiety is attached to the alkyl group, and wherein the "alkyl" portions are as defined herein.
  • Hydroxymethylalkynyl refers to the group -alkynyl-CH 2 -OH, wherein the parent moiety is attached to the alkynyl group, and wherein the "alkynyl” portion is as defined herein.
  • Heteroarylalkyl refers to the group -alkyl-heteroaryl, wherein the parent moiety is attached to the alkyl group, and wherein the "alkyl” and heteroaryl portions are as defined herein.
  • Heterocycloalkylaminoalkyl refers to the group -alkyl-N(H)-alkyl- heterocylcoalkyl, wherein the parent moiety is attached to the alkyl group, and wherein the "alkyl” and “heterocycloalkyl” portions are as defined herein.
  • Cycloalkylaminoalkyl refers to the group -alkyl-N(H)-cycloalkyl, wherein the parent moiety is attached to the alkyl group, and wherein the "alkyl" and
  • cycloalkyl portions are as defined herein.
  • Heterocycloalkylalkylaminoalkyl refers to the group -alkyl-N(H)-alkyl- heterocycloalkyl, wherein the parent moiety is attached to the alkyl group, and wherein the "alkyl” and “heterocycloalkyl” portions are as defined herein.
  • Arylalkylaminoalkyl refers to the group -alkyl-N(H)-alkyl-aryl, wherein the parent moiety is attached to the alkyl group, and wherein the "alkyl” and “aryl” portions are as defined herein.
  • Alkylheterocycloalkyl refers to the group -alkyl-heterocycoalkyl, wherein the parent moiety is attached to the alkyl group, and wherein the "alkyl" and
  • Heterocycloalkyl portions are as defined herein.
  • Heteroarylaminoalkyl refers to the group -alkyl-N(H)-heteroaryl, wherein the parent moiety is attached to the alkyl group, and wherein the "alkyl” and “heteroaryl” portions are as defined herein.
  • Arylamino refers to the group -N(H)-aryl, wherein the parent moiety is attached to the amino group, and wherein the "aryl” portion is as defined herein.
  • Aryloxyalkyl refers to the group -alkyl-O-aryl, wherein the parent moiety is attached to the alkyl group, and wherein the "alkyl” and “aryl” portions are as defined herein.
  • Heteroarylalkyl refers to the group -alkyl-heteroaryl, wherein the parent moiety is attached to the alkyl group, and wherein the " alkyl” and “heteroaryl” portions are as defined herein.
  • Alkylpiperazinylcarbonyl refers to the group -C(O)-piperazinyl-alkyl, wherein the parent moiety is attached to the carbonyl group, and wherein the "alkyl” portion is as defined herein.
  • Alkylheterocycloakylarylalkoxyalkyl refers to the group -alkyl-O- alkyl-aryl-heterocycloalkyl-alkyl, wherein the parent moiety is attached to the alkyl group, and wherein the "aryl,” “aryl” and “heterocycloalkyl” portions are as defined herein.
  • Alkylheteroarylalkoxyalkyl refers to the group -alkyl-O-alkyl- heteroaryl-alkyl, wherein the parent moiety is attached to the alkyl group, and wherein the "aryl” and “heteroaryl” portions are as defined herein.
  • Dialkylaminoalkylamino refers to the group -N(H)-alkyl-N(alkyl) 2 , wherein the parent moiety is attached to the amino group, and wherein the "alkyl” portions are as defined herein.
  • Heterocycloalkylalkylamino refers to the group -N(H)-alkyl- heterocycloalkyl, wherein the parent moiety is attached to the amino group, and wherein the "alkyl” and “heterocycloalkyl” portions are as defined herein.
  • Heteroarylamino refers to the group -N(H)-heteroaryl, wherein the parent moiety is attached to the amino group, and wherein the "heteroaryl” portion is as defined herein.
  • Heterocycloalkylalkoxyalkyl refers to the group -alkyl-O-alkyl- heterocycloalkyl, wherein the parent moiety is attached to the alkyl group, and wherein the "alkyl” and heterocycloalkyl portions are as defined herein.
  • Carboxyalkyl refers to the group -alkyl-C(O)-OH, wherein the parent moiety is attached to the alkyl group, and wherein the "alkyl” portion is as defined herein.
  • Heterocycloalkyloxyalkyl refers to the group -alkyl-O- heterocycloalkyl, wherein the parent moiety is attached to the alkyl group, and wherein the "alkyl” and “heterocycloalkyl” portions are as defined herein.
  • Alkoxycarbonylalkyl refers to the group -alkyl-C(O)-O-alkyl, wherein the parent moiety is attached to the alkyl group, and wherein the "alkyl” portions are as defined herein.
  • Arylalkoxy refers to the group -alkyl-O-aryl, wherein the parent moiety is attached to the alkyl group, and wherein the "alkyl” and “aryl” portions are as defined herein.
  • Spiro-heterocycloalkyl refers to the group:
  • Arylalkylaminocarbonyl refers to the group -C(O)-N(H)-alkyl-aryl, wherein the parent moiety is attached to the carbonyl group, and wherein the "alkyl” and “aryl” portions are as defined herein.
  • Heterocycloalkylalkylamino refers to the group -N(H)-alkyl- heterocycloalkyl, wherein the parent moiety is attached to the amino group, and wherein the "alkyl” and “heterocycloalkyl” portions are as defined herein.
  • Dialkylaminoalkylcarbonyl refers to the group -C(O)-alkyl-N(alkyl) 2 , wherein the parent moiety is attached to the carbonyl group, and wherein the "alkyl” portions are as defined herein.
  • Dialkylaminocarbonylalkyl refers to the group -alkyl-C(O)-N(alkyl) 2 , wherein the parent moiety is attached to the alkyl group, and wherein the "alkyl” portions are as defined herein.
  • “Heterocycloalkylcarbonyl” refers to the group -C(O)-heterocycloalkyl, wherein the parent moiety is attached to the carbonyl group, and wherein the "heterocycloalkyl” portion is as defined herein.
  • Alkylthio refers to the group -S-alkyl, wherein the parent moiety is attached to the thio group (-S-), and wherein the "alkyl” portion is as defined herein.
  • Alkylsulfonyl refers to the group -S(O) 2 -alkyl, wherein the parent moiety is attached to the sulfonyl group [-S(O) 2 -], and wherein the "alkyl” portion is as defined herein
  • Alkylheterocycloalkyl refers to the group -alkyl-heterocycloalkyl, wherein the parent moiety is attached to the alkyl group, and wherein the "alkyl" and
  • heterocycloalkyl portions are as defined herein.
  • Alkoxycarbonylheterocycloalkylaminoalkyl refers to the group -alkyl- N(H)-heterocycloalkyl-C(O)-O-alkyl, wherein the parent moiety is attached to the alkyl group, and wherein the "alkyl” and “heterocycloalkyl” portions are as defined herein.
  • Heterocycloalkylaminoalkyl refers to the group -alkyl-N(H)- heterocycloalkyl, wherein the parent moiety is attached to the alkyl group, and wherein the "alkyl” and “heterocycloalkyl” portions are as defined herein.
  • Aminoalkylaminocarbonyl refers to the group -N(H)-alkyl- heterocycloalkyl, wherein the parent moiety is attached to the amino group, and wherein the "alkyl” and “heterocycloalkyl” portions are as defined herein.
  • Heteroarylcarbonyl refers to the group -C(O)-heteroaryl, wherein the parent moiety is attached to the carbonyl group, and wherein the "heteroaryl” portion is defined herein.
  • Arylalkylcarbonyl refers to the group -C(O)-alkyl-aryl, wherein the parent moiety is attached to the carbonyl group, and wherein the "alkyl” and “aryl” portions are as defined herein.
  • Arylcarbonyl refers to the group -C(O)-aryl, wherein the parent moiety is attached to the carbonyl group, and wherein the "aryl” portion is defined herein.
  • ⁇ lkylcarbonyl refers to the group -C(O)-alkyl, wherein the parent moiety is attached to the carbonyl group, and wherein the "alkyl” portion is defined herein.
  • Alkoxyalkylcarbonyl refers to the group -C(O)-alkyl-O-alkyl, wherein the parent moiety is attached to the carbonyl group, and wherein the "alkyl” portions are as defined herein.
  • Spiro-cycloalkyl refers to the group:
  • Cyc represents a cycloalkyl group and P represents the parent moiety, wherein the cycloalkyl portion is as defined herein.
  • saturated bridged ring system refers to a bicyclic or polycyclic ring system that is not aromatic. Such a system can contain isolated or conjugated unsaturation, but not aromatic or heteroaromatic rings in its core structure (but can have aromatic substitution thereon). For example, hexahydro-furo[3,2-b]furan, 2,3,3a,4,7,7a-hexahydro-lH-indene, 7-aza-bicyclo[2.2.1]-heptane, and l,2,3,4,4a,5,8,8a-octahydro-naphthalene are all included in the class "saturated bridged ring system.
  • Spirocyclyl or "spirocyclic ring” refers to a ring originating from a particular annular carbon of another ring.
  • a ring atom of a saturated bridged ring system (rings B and B'), but not a bridgehead atom, can be a shared atom between the saturated bridged ring system and a spirocyclyl (ring A) attached thereto.
  • a spirocyclyl can be carbocyclic or heteroalicyclic.
  • Some of the compounds of the disclosure can have imino, amino, oxo or hydroxy substituents off aromatic heterocyclyl systems.
  • imino, amino, oxo or hydroxy substituents can exist in their corresponding tautomeric form, i.e., amino, imino, hydroxy or oxo, respectively.
  • Animal for the purposes of this disclosure includes humans (including patients receiving treatment) and other animals, particularly mammals, and other organisms. Thus, the methods are applicable to both human therapy and veterinary applications.
  • the patient is a mammal, and in a most preferred embodiment the patient is human.
  • Kinase-dependent diseases or conditions refer to pathologic conditions that depend on the activity of one or more protein kinases.
  • Kinases either directly or indirectly participate in the signal transduction pathways of a variety of cellular activities including proliferation, adhesion, migration, differentiation and invasion.
  • Diseases associated with kinase activities include tumor growth, the pathologic neovascularization that supports solid tumor growth, and associated with other diseases where excessive local vascularization is involved such as ocular diseases (diabetic retinopathy, age-related macular degeneration, and the like) and inflammation (psoriasis, rheumatoid arthritis, and the like).
  • phosphatases can also play a role in "kinase-dependent diseases or conditions" as cognates of kinases; that is, kinases phosphorylate and phosphatases dephosphorylate, for example protein substrates. Therefore compounds of this disclosure, while modulating kinase activity as described herein, can also modulate, either directly or indirectly, phosphatase activity. This additional modulation, if present, can be synergistic (or not) to activity of compounds of this disclosure toward a related or otherwise interdependent kinase or kinase family. In any case, as stated previously, the compounds of this disclosure are useful for treating diseases characterized in part by abnormal levels of cell proliferation (i.e. tumor growth), programmed cell death (apoptosis), cell migration and invasion and angiogenesis associated with tumor growth.
  • abnormal levels of cell proliferation i.e. tumor growth
  • apoptosis programmed cell death
  • “Therapeutically effective amount” is an amount of a compound of this disclosue, that when administered to a patient, ameliorates a symptom of the disease.
  • the amount of a compound of this disclosure which constitutes a “therapeutically effective amount” will vary depending on the compound, the disease state and its severity, the age of the patient to be treated, and the like. The therapeutically effective amount can be determined routinely by one of ordinary skill in the art having regard to their knowledge and to this disclosure.
  • Cardiac sarcoma (angiosarcoma, fibrosarcoma, rhabdomyosarcoma, liposarcoma), myxoma, rhabdomyoma, fibroma, lipoma and teratoma;
  • Lung bronchogenic carcinoma (squamous cell, undifferentiated small cell, undifferentiated large cell, adenocarcinoma), alveolar (bronchiolar) carcinoma, bronchial adenoma, sarcoma, lymphoma, chondromatous hamartoma, mesothelioma;
  • Gastrointestinal esophagus (squamous cell carcinoma, adenocarcinoma, leiomyosarcoma, lymphoma), stomach (carcinoma, lymphoma, leioma, leioma, leioma, f
  • a "pharmaceutically acceptable salt” of a compound means a salt that is pharmaceutically acceptable and that possesses the desired pharmacological activity of the parent compound. It is understood that the pharmaceutically acceptable salts are non-toxic. Additional information on suitable pharmaceutically acceptable salts can be found in Remington 's Pharmaceutical Sciences, 17 th ed., Mack Publishing Company, Easton, PA, 1985, which is incorporated herein by reference or S. M. Berge, et al, "Pharmaceutical Salts,” J. Pharm. ScL, 1977;66:1-19 both of which are incorporated herein by reference.
  • Examples of pharmaceutically acceptable acid addition salts include those formed with inorganic acids such as hydrochloric acid, hydrobromic acid, sulfuric acid, nitric acid, phosphoric acid, and the like; as well as organic acids such as acetic acid, trifluoroacetic acid, propionic acid, hexanoic acid, cyclopentanepropionic acid, glycolic acid, pyruvic acid, lactic acid, oxalic acid, maleic acid, malonic acid, succinic acid, fumaric acid, tartaric acid, citric acid, benzoic acid, cinnamic acid, 3-(4- hydroxybenzoyl)benzoic acid, mandelic acid, methanesulfonic acid, ethanesulfonic acid, 1 ,2-ethanedisulfonic acid, 2-hydroxyethanesulfonic acid, benzenesulfonic acid,
  • inorganic acids such as hydrochloric acid, hydrobromic acid, sulfuric acid, n
  • Examples of a pharmaceutically acceptable base addition salts include those formed when an acidic proton present in the parent compound is replaced by a metal ion, such as sodium, potassium, lithium, ammonium, calcium, magnesium, iron, zinc, copper, manganese, aluminum salts and the like.
  • a metal ion such as sodium, potassium, lithium, ammonium, calcium, magnesium, iron, zinc, copper, manganese, aluminum salts and the like.
  • Preferable salts are the ammonium, potassium, sodium, calcium, and magnesium salts.
  • Salts derived from pharmaceutically acceptable organic non-toxic bases include, but are not limited to, salts of primary, secondary, and terriary amines, substituted amines including naturally occurring substituted amines, cyclic amines and basic ion exchange resins.
  • organic bases include isopropylamine, trimethylamine, diethylamine, triethylamine, tripropylamine, ethanolamine, 2-dimethylaminoethanol, 2- diethylaminoethanol, dicyclohexylamine, lysine, arginine, histidine, caffeine, procaine, hydrabamine, choline, betaine, ethylenediamine, glucosamine, methylglucamine, theobromine, purines, piperazine, piperidine, N-ethylpiperidine, tromethamine, JV-methylglucamine, polyamine resins, and the like.
  • Exemplary organic bases are isopropylamine, diethylamine, ethanolamine, trimethylamine, dicyclohexylamine, choline, and caffeine.
  • “Prodrug” refers to compounds that are transformed (typically rapidly) in vivo to yield the parent compound of the above formulae, for example, by hydrolysis in blood. Common examples include, but are not limited to, ester and amide forms of a compound having an active form bearing a carboxylic acid moiety.
  • Examples of pharmaceutically acceptable esters of the compounds of this disclosure include, but are not limited to, alkyl esters (for example with between about one and about six carbons) the alkyl group is a straight or branched chain.
  • Acceptable esters also include cycloalkyl esters and arylalkyl esters such as, but not limited to benzyl.
  • Examples of pharmaceutically acceptable amides of the compounds of this disclosure include, but are not limited to, primary amides, and secondary and tertiary alkyl amides (for example with between about one and about six carbons).
  • Amides and esters of the compounds of this disclosure can be prepared according to conventional methods. A thorough discussion of prodrugs is provided in T. Higuchi and V. Stella, "Pro-drugs as Novel Delivery Systems," Vol. 14 of the A.C.S. Symposium Series, and in
  • Methodabolite refers to the break-down or end product of a compound or its salt produced by metabolism or biotransformation in the animal or human body; for example, biotransformation to a more polar molecule such as by oxidation, reduction, or hydrolysis, or to a conjugate (see Goodman and Gilman, "The Pharmacological Basis of Therapeutics” 8.sup.th Ed., Pergamon Press, Gilman et al.. (eds), 1990 for a discussion of biotransformation).
  • the metabolite of a compound of this disclosure or its salt can be the biologically active form of the compound in the body.
  • a prodrug can be used such that the biologically active form, a metabolite, is released in vivo.
  • a biologically active metabolite is discovered serendipitously, that is, no prodrug design per se was undertaken.
  • An assay for activity of a metabolite of a compound of this disclosure is known to one of skill in the art in light of the present disclosure.
  • the compounds of this disclosure also include N-oxide derivatives and protected derivatives of compounds of Formula I, II or III.
  • compounds of Formula I when compounds of Formula I contain an oxidizable nitrogen atom, the nitrogen atom can be converted to an N-oxide by methods well known in the art.
  • compounds of Formula I when compounds of Formula I contain groups such as hydroxy, carboxy, thiol or any group containing a nitrogen atom(s), these groups can be protected with a suitable "protecting group” or "protective group”.
  • a comprehensive list of suitable protective groups can be found in T.W. Greene, Protective Groups in Organic Synthesis, John Wiley & Sons, Inc. 1991, the disclosure of which is incorporated herein by reference in its entirety.
  • the protected derivatives of compounds of Formula I can be prepared by methods well known in the art.
  • Treating" or "treatment” of a disease, disorder, or syndrome includes (i) preventing the disease, disorder, or syndrome from occurring in a human, i.e. causing the clinical symptoms of the disease, disorder, or syndrome not to develop in an animal that can be exposed to or predisposed to the disease, disorder, or syndrome but does not yet experience or display symptoms of the disease, disorder, or syndrome; (ii) inhibiting the disease, disorder, or syndrome, i.e., arresting its development; and (iii) relieving the disease, disorder, or syndrome, i.e., causing regression of the disease, disorder, or syndrome.
  • Such suitable x-ray quality crystals can be used as part of a method of identifying a candidate agent capable of binding to and modulating the activity of kinases.
  • Such methods can be characterized by the following aspects: a) introducing into a suitable computer program, information defining a ligand binding domain of a kinase in a conformation (e.g.
  • aspects a-d are not necessarily carried out in the aforementioned order. Such methods can further entail: performing rational drug design with the model of the three-dimensional structure, and selecting a potential candidate agent in conjunction with computer modeling.
  • Such methods can further entail: employing a candidate agent, so-determined to fit spatially into the ligand binding domain, in a biological activity assay for kinase modulation, and determining whether said candidate agent modulates kinase activity in the assay. Such methods can also include administering the candidate agent, determined to modulate kinase activity, to a mammal suffering from a condition treatable by kinase modulation, such as those described above.
  • compounds disclosed herein can be used in a method of evaluating the ability of a test agent to associate with a molecule or molecular complex comprising a ligand binding domain of a kinase.
  • a method of evaluating the ability of a test agent to associate with a molecule or molecular complex comprising a ligand binding domain of a kinase can be characterized by the following aspects: a) creating a computer model of a kinase binding pocket using structure coordinates obtained from suitable x-ray quality crystals of the kinase, b) employing computational algorithms to perform a fitting operation between the test agent and the computer model of the binding pocket, and c) analyzing the results of the fitting operation to quantify the association between the test agent and the computer model of the binding pocket.
  • administration can preferably be by the oral route.
  • Administration of the compounds of this disclosure, or their pharmaceutically acceptable salts, in pure form or in an appropriate pharmaceutical composition can be carried out via any of the accepted modes of administration or agents for serving similar utilities.
  • administration can be, for example, orally, nasally, parenterally (intravenous, intramuscular, or subcutaneous), topically, transdermally, intravaginally, intravesically, intracistemally, or rectally, in the form of solid, semi-solid, lyophilized powder, or liquid dosage forms, such as for example, tablets, suppositories, pills, soft elastic and hard gelatin capsules, powders, solutions, suspensions, or aerosols, or the like, preferably in unit dosage forms suitable for simple administration of precise dosages.
  • compositions will include a conventional pharmaceutical carrier or excipient and a compound of this disclosure as the/an active agent, and, in addition, can include carriers and adjuvants, etc.
  • Adjuvants include preserving, wetting, suspending, sweetening, flavoring, perfuming, emulsifying, and dispensing agents. Prevention of the action of microorganisms can be ensured by various antibacterial and antifungal agents, for example, parabens, chlorobutanol, phenol, sorbic acid, and the like. It can also be desirable to include isotonic agents, for example sugars, sodium chloride, and the like.
  • Prolonged absorption of the injectable pharmaceutical form can be brought about by the use of agents delaying absorption, for example, aluminum monostearate and gelatin.
  • a pharmaceutical composition of the compounds in this disclosure can also contain minor amounts of auxiliary substances such as wetting or emulsifying agents, pH buffering agents, antioxidants, and the like, such as, for example, citric acid, sorbitan monolaurate, triethanolamine oleate, butylalted hydroxytoluene, etc.
  • auxiliary substances such as wetting or emulsifying agents, pH buffering agents, antioxidants, and the like, such as, for example, citric acid, sorbitan monolaurate, triethanolamine oleate, butylalted hydroxytoluene, etc.
  • formulations depend on various factors such as the mode of drug administration (e.g., for oral administration, formulations in the form of tablets, pills or capsules are preferred) and the bioavailability of the drug substance.
  • pharmaceutical formulations have been developed especially for drugs that show poor bioavailability based upon the principle that bioavailability can be increased by increasing the surface area i.e., decreasing particle size.
  • U.S. Pat. No. 4,107,288 describes a pharmaceutical formulation having particles in the size range from 10 to 1,000 nm in which the active material is supported on a crosslinked matrix of macromolecules.
  • 5,145,684 describes the production of a pharmaceutical formulation in which the drug substance is pulverized to nanoparticles (average particle size of 400 nm) in the presence of a surface modifier and then dispersed in a liquid medium to give a pharmaceutical formulation that exhibits remarkably high bioavailability.
  • compositions suitable for parenteral injection can comprise physiologically acceptable sterile aqueous or nonaqueous solutions, dispersions, suspensions or emulsions, and sterile powders for reconstitution into sterile injectable solutions or dispersions.
  • suitable aqueous and nonaqueous carriers, diluents, solvents or vehicles include water, ethanol, polyols (propyleneglycol, polyethyleneglycol, glycerol, and the like), suitable mixtures thereof, vegetable oils (such as olive oil) and injectable organic esters such as ethyl oleate.
  • Proper fluidity can be maintained, for example, by the use of a coating such as lecithin, by the maintenance of the required particle size in the case of dispersions and by the use of surfactants.
  • One preferable route of administration is oral, using a convenient daily dosage regimen that can be adjusted according to the degree of severity of the disease- state to be treated.
  • Solid dosage forms for oral administration include capsules, tablets, pills, powders, and granules.
  • the active compound is admixed with at least one inert customary excipient (or carrier) such as sodium citrate or dicalcium phosphate or
  • fillers or extenders as for example, starches, lactose, sucrose, glucose, mannitol, and silicic acid
  • binders as for example, cellulose derivatives, starch, alignates, gelatin, polyvinylpyrrolidone, sucrose, and gum acacia
  • humectants as for example, glycerol
  • disintegrating agents as for example, agar-agar, calcium carbonate, potato or tapioca starch, alginic acid, croscarmellose sodium, complex silicates, and sodium carbonate
  • solution retarders as for example paraffin
  • absorption accelerators as for example
  • Solid dosage forms can be prepared with coatings and shells, such as enteric coatings and others well known in the art. They can contain pacifying agents, and can also be of such composition that they release the active compound or compounds in a certain part of the intestinal tract in a delayed manner. Examples of embedded compositions that can be used are polymeric substances and waxes. The active compounds can also be in microencapsulated form, if appropriate, with one or more of the above-mentioned excipients. [00223] Liquid dosage forms for oral administration include pharmaceutically acceptable emulsions, solutions, suspensions, syrups, and elixirs.
  • Such dosage forms are prepared, for example, by dissolving, dispersing, etc., a compound(s) of this disclosure, or a pharmaceutically acceptable salt thereof, and optional pharmaceutical adjuvants in a carrier, such as, for example, water, saline, aqueous dextrose, glycerol, ethanol and the like; solubilizing agents and emulsifiers, as for example, ethyl alcohol, isopropyl alcohol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propyleneglycol, 1,3-butyleneglycol, dimethylformamide; oils, in particular, cottonseed oil, groundnut oil, corn germ oil, olive oil, castor oil and sesame oil, glycerol, tetrahydrofurfuryl alcohol, polyethyleneglycols and fatty acid esters of sorbitan; or mixtures of these substances, and the like, to thereby form a solution
  • Suspensions in addition to the active compounds, can contain suspending agents, as for example, ethoxylated isostearyl alcohols, polyoxyethylene sorbitol and sorbitan esters, microcrystalline cellulose, aluminum metahydroxide, bentonite, agar- agar and tragacanth, or mixtures of these substances, and the like.
  • suspending agents as for example, ethoxylated isostearyl alcohols, polyoxyethylene sorbitol and sorbitan esters, microcrystalline cellulose, aluminum metahydroxide, bentonite, agar- agar and tragacanth, or mixtures of these substances, and the like.
  • compositions for rectal administrations are, for example, suppositories that can be prepared by mixing the compounds of this disclosure with, for example, suitable non-irritating excipients or carriers such as cocoa butter, polyethyleneglycol or a suppository wax, which are solid at ordinary temperatures but liquid at body temperature and therefore, melt while in a suitable body cavity and release the active component therein.
  • suitable non-irritating excipients or carriers such as cocoa butter, polyethyleneglycol or a suppository wax, which are solid at ordinary temperatures but liquid at body temperature and therefore, melt while in a suitable body cavity and release the active component therein.
  • Dosage forms for topical administration of a compound of this disclosure include ointments, powders, sprays, and inhalants.
  • the active component is admixed under sterile conditions with a physiologically acceptable carrier and any preservatives, buffers, or propellants as can be required.
  • Ophthalmic formulations, eye ointments, powders, and solutions are also contemplated for the comounds in this disclosure.
  • Compressed gases can be used to disperse a compound of this disclosure in aerosol form. Inert gases suitable for this purpose are nitrogen, carbon dioxide, etc.
  • the pharmaceutically acceptable compositions will contain about 1% to about 99% by weight of a compound(s) of this disclosure, or a pharmaceutically acceptable salt thereof, and 99% to 1% by weight of a suitable pharmaceutical excipient.
  • the composition will be between about 5% and about 75% by weight of a compound(s) of this disclosure, or a pharmaceutically acceptable salt thereof, with the rest being suitable pharmaceutical excipients.
  • Actual methods of preparing such dosage forms are known, or will be apparent, to those skilled in this art; for example, see Remington's Pharmaceutical
  • composition to be administered will, in any event, contain a therapeutically effective amount of a compound of this disclosure, or a pharmaceutically acceptable salt thereof, for treatment of a disease-state in accordance with the teachings of this disclosure.
  • the compounds of this disclosure are administered in a therapeutically effective amount which will vary depending upon a variety of factors including the activity of the specific compound employed, the metabolic stability and length of action of the compound, the age, body weight, general health, sex, diet, mode and time of administration, rate of excretion, drug combination, the severity of the particular disease-states, and the host undergoing therapy.
  • the compounds of this disclosure can be administered to a patient at dosage levels in the range of about 0.1 to about 1,000 mg per day. For a normal human adult having a body weight of about 70 kilograms, a dosage in the range of about 0.01 to about 100 mg per kilogram of body weight per day is an example. The specific dosage used, however, can vary.
  • the dosage can depend on a number of factors including the requirements of the patient, the severity of the condition being treated, and the pharmacological activity of the compound being used.
  • the determination of optimum dosages for a particular patient is well known to one of ordinary skill in the art.
  • compositions will include a conventional pharmaceutical carrier or excipient and a compound of this disclosure as the/an active agent, and, in addition, can include other medicinal agents and pharmaceutical agents.
  • Compositions of the compounds in this disclosure can be used in combination with anticancer and/or other agents that are generally administered to a patient being treated for cancer, e.g. surgery, radiation and/or chemotherapeutic agent(s).
  • Chemotherapeutic agents that can be useful for administration in combination with compounds of Formula I in treating cancer include alkylating agents, platinum containing agents.
  • combination products employ the compounds of this disclosure within the dosage range described above and the other pharmaceutically active agent(s) within its approved dosage range.
  • Compounds of this disclosure can alternatively be used sequentially with known pharmaceutically acceptable agent(s) when a combination formulation is inappropriate.
  • Representative pharmaceutical formulations containing the compounds disclosed herein are described below. Synthetic Procedures
  • the compounds disclosed herein, or their pharmaceutically acceptable salts can have asymmetric carbon atoms, oxidized sulfur atoms or quaternized nitrogen atoms in their structure.
  • the compounds disclosed herein and their pharmaceutically acceptable salts can exist as single stereoisomers, racemates, and as mixtures of enantiomers and diastereomers.
  • the compounds disclosed herein can also exist as geometric isomers.
  • Enantiomers can be resolved by methods known to one of ordinary skill in the art, for example by: formation of diastereoisomeric salts or complexes which can be separated, for example, by crystallization; via formation of diastereoisomeric derivatives which can be separated, for example, by crystallization, selective reaction of one enantiomer with an enantiomer-specific reagent, for example enzymatic oxidation or reduction, followed by separation of the modified and unmodified enantiomers; or gas-liquid or liquid chromatography in a chiral environment, for example on a chiral support, such as silica with a bound chiral ligand or in the presence of a chiral solvent.
  • enantiomer can be synthesized by asymmetric synthesis using optically active reagents, substrates, catalysts or solvents or by converting on enantiomer to the other by asymmetric transformation.
  • enantiomer enriched in a particular enantiomer, the major component enantiomer can be further enriched (with concomitant loss in yield) by recrystallization.
  • the compounds of this disclosure can exist in unsolvated as well as solvated forms with pharmaceutically acceptable solvents such as water, ethanol, and the like.
  • the solvated forms are considered equivalent to the unsolvated forms for the purposes of the compounds of this disclosure.
  • IR spectra were collected by reflectance on a Perkin Elmer SpectrumTM 100 FT-IR.
  • 1 H NMR were collected on a Varian 400 MHz with Mercury and Mercury consoles.
  • 8-bromo-2- ([(U -dimethyl-2-piperidin- 1 -ylethyl)amino]methyl
  • the resulting slurry was diluted with additional anhydrous ethanol (10 ml) and the resulting slurry was heated at 80 0 C for 16 hours.
  • the resulting precipitate was filtered off and washed with ethyl acetate (2 x 50 ml) and methanol (2 x 5 ml) to give 8- bromo-2-(2-hydroxyphenyl)[l]benzofuro[3,2-d]pyrimidin-4(3H)-one.
  • N- ⁇ (3S)-l-[(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2- yl)methyl]pyrrolidin-3-yl ⁇ acetamide was synthesized in a manner similar to Example 1, Compound 2, wherein piperidine was substituted with (3S)-(-)-3- acetamidopyrrolidine. Purification by preparative HPLC (reverse-phase, acetonitrile/water with 0.01% ammonium acetate), followed by concentration in vacuo and lyophilization afforded the title compound as a white solid.
  • 8-bromo-2-[(piperidin-4-ylamino)methyl][l]benzofuro[3,2-d]pyrimidin- 4(3H)-one was synthesized in a manner similar to Example 2, wherein (4-(4- methylpiperazin-l-yl)phenyl)methanamine was substituted with tert-butyl 4- aminopiperidine-1-carboxylate.
  • tert-butyl 8-bromo-2-[(piperidin-4- ylamino)methyl][l]benzofuro[3,2-d]pyrimidin-4(3H)-one-carboxylate was taken in 4 mL methanol and 2mL of 4N HCl/dioxanes.
  • 8-bromo-2- ⁇ [4-(N,N-diethylglycyl)piperazin-l- yl]methyl ⁇ [l]benzofuro[3,2-d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 2, wherein (4-(4-methylpiperazin-l-yl)phenyl)methanamine was substituted with 2-(diethylamino)-l-(piperazin-l-yl)ethanone.
  • N-2-[(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2- yl)methyl]-2-methylalaninamide was synthesized in a manner similar to Example 2, wherein (4-(4-methylpiperazin-l-yl)phenyl)methanamine was substituted with methyl- ⁇ -aminoisobutyrate.
  • N-[(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2- yl)methyl]-2-methylalanine was synthesized in a manner similar to Example 2, wherein (4-(4-methylpiperazin-l-yl)phenyl)methanamine was substituted with methyl- ⁇ -aminoisobutyrate.
  • 8-bromo-2- ([(U -dimethyl-2-pyrrolidin- 1 -ylethyl)amino "
  • R 3a is as defined in the disclosure above and R15 is described within the compounds within this example.
  • 8-bromo-2-chlorobenzofuro[3,2-d]pyrimidin-4(3H)-one 27 To a solution of 8-bromo-2,4-dichlorobenzofuro[3,2-d]pyrimidine (26) (0.372 g, 1.12 mmol) in 5 mL dioxane was added 2N NaOH (2 mL) and heated to 85 0C for 10 minutes. The reaction mixture was cooled to room temperature, acidified with IN HCl (10 mL) and extracted with ethyl acetate (2 x 100 mL).
  • R3 a is as defined in the disclosure above and R 5 is described within the compounds within this example.
  • the precipitate was suspended in 3 mL of dimethylacetamide and heated to 150 0 C for 2 hr. Sodium bisulfite (80 mg, 0.76 mmol) was added and the reaction was heated at 150 0 C for another 45 min. After cooling, the reaction mixture was filtered and the filtrate was diluted with 5 mL of water. The precipitate was filtered, washed with 1 mL of methanol and dried under vacuum to give 19 mg of 8-bromo-2-(2-chlorophenyl)[l]benzofuro[3,2-d]pyrimidin- 4(3H)-one.
  • 8-Bromo-2-(2-bromophenyl)rilbenzofuror3,2-dlpyrimidin-4(3H)-one 8-Bromo-2-(2-bromophenyl)[l]benzofuro[3,2-d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 8, wherein 2-chlorobenzaldehyde was substituted with 2-bromobenzaldehyde.
  • 8-bromo-2-(2-iodophenyl)[llbenzofuro[3,2-dlpyrimidin-4(3H)-one 8-bromo-2-(2-iodophenyl)[l]benzofuro[3,2-d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 8, wherein 2-chlorobenzaldehyde was substituted with 2-iodobenzaldehyde.
  • 8-bromo-2-(3-bromopyridin-4-yl)[ 1 ]benzofuro[3,2-d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 8, wherein 2-chlorobenzaldehyde was substituted with 3-bromo-4-pyridinecarboxaldehyde.
  • 8-Bromo-2-pyridin-2-yl[llbenzofuro[3,2-dlpyrimidin-4(3H)-one was synthesized manner similar to Example 8, wherein 2-chlorobenzaldehyde was substituted with pyridine-2-carboxaldehyde.

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Abstract

A compound according to formula I: or a pharmaceutically acceptable salt thereof; wherein R1, R2, R3a, R3b, R3c and R3d are as defined in the specification, pharmaceutical compositions thereof, and methods of use thereof.

Description

BENZOFUROPYRIMIDINONES AS PROTEIN KINASE INHIBITORS
CROSS-REFERENCE TO RELATED APPLICATIONS
[0001] This application claims priority to U.S. provisional application 61/008,907, filed December 21, 2007, and U.S. provisional application 61/070,971 filed on March 25, 2008.
FIELD OF THE INVENTION
[0002] This disclosure relates to certain benzofuropyrimidinone compounds. In particular, this disclosure relates to certain benzofuropyrimidinone compounds useful as inhibitors of protein kinases.
BACKGROUND OF THE INVENTION
[0003] The PIM protein kinase family which consists of the closely related PIM- 1, 2, and 3, have been implicated in diverse biological processes such as cell survival, proliferation, and differentiation. PIM-I is involved in a number of signaling pathways that are highly relevant to tumorigenesis [reviewed in Bachmann & Moray, Internat. J. Biochem. Cell Biol, 37, 726-730 (2005)]. Many of these are involved in cell cycle progression and apoptosis. It has been shown that PIM-I acts as an anti- apoptotic factor via inactivation of the pro-apoptotic factor Bad. This finding suggested a direct role of PIM-I in preventing cell death since the inactivation of Bad can enhance Bcl-2 activity and thereby promotes cell survival [Aho et al., FEBS Letters, 571, 43-49 (2004)]. PIM-I has also been recognized as a positive regulator of cell cycle progression. PIM-I binds and phosphorylates CDC25A, which leads to an increase in its phosphatase activity and promotion of Gl /S transition [reviewed in Losman et al., JBC, 278, 4800-4805 (1999)]. In addition, the cyclin kinase inhibitor p21Waf which inhibits Gl /S progression was found to be inactivated by PIM-I [Wang et al., Biochim. Biophys. Act. 1593, 45-55 (2002)]. Furthermore, by means of phosphorylation, Pim-1 inactivates C-TAKl and activates Cdc25C which results in acceleration of G2/M transition [Bachman et al., JBC, 279, 48319-48 (2004)]. [0004] PIM-I appears to be an essential player in hematopoetic proliferation. Kinase active PIM-I is required for the gpl30-mediated STAT3 proliferation signal [Hirano et al., Oncogene 19, 2548-2556, (2000)]
[0005] PIM-I is overexpressed or even mutated in a number of tumors and different types of tumor cell lines and leads to genomic instability. Examples for a possible involvement of PIM-I in human tumors are prostate cancer, oral cancer, and Burkitt lymphoma (Gaidano & Dalla Faver, 1993). All these findings point to an important role of PIM-I in the initiation and progression of human cancer, and it appears that small molecule inhibition of PIM-I activity is a promising therapeutic strategy. Finally, PIM -2 and PIM-3 have overlapping functions with PIM-I and inhibition of more than one isoform may provide additional therapeutic benefits. [0006] CDC7, a serine/threonine kinase, plays an essential role in initiation of DNA replication in eukaryotic cells (Jiang et al, EMBO J 18:5703 (1999)). After assembly of the pre -replication complex to the replication origin, the CDC7 kinase phosphorylates MCM (minichromosome maintenance) proteins and allows for recruitment of CDC45 and DNA polymerase thereby initiating DNA replication (Kim et al., Mutation Research 532:29(2003)). CDC7 requires association with one of its co factors, ASK (also known as DBF4) or ASKLl (also known as Drfl), for kinase activation (Ogino et al., J Biol Chem 276:31376 (2001); Sato et al., Genes to Cells 8:451 (2003); Montagnoli et al., EMBO J 21 :3171 (2002); Yoshizawa-Sugata et al., J Biol Chem 280, 13062 (2005)). Mice deficient for CDC7 die between day 3.5 and 6.5 indicating that CDC7 plays a role for early embryonic development (Kim et al., EMBO J 21 :2168 (2002)). Conditional knock-down of CDC7 in mouse ES cell lines (CDC7-/-tg) revealed immediate inhibition of cell proliferation, rapid cessation of DNA synthesis and arrest in S phase progression (Kim et al. (2002)). CDC7 has been implicated in DNA damage checkpoint signaling in response to Etoposide treatment or DNA single strand breaks (Costanzo et al., J MoI Cell 11 :203 (2003)). A role for CDC7 in DNA damage response is supported by the observation that CDC7 depleted mouse ES cells accumulate RAD51 foci in the nucleus (Kim et al. (2002)). Deletion of CDC7 in yeast results in hypersensitivity to hydroxyurea treatment (Weinreich et al., EMBO J 18:5334 (1999)).
[0007] The serine/threonine kinase CDC7 plays an important role in the initiation of DNA replication and recently has been implicated in S phase checkpoint signaling(reviewed in Kim, Yamada and Masai, "Functions of mammalian CDC7 kinase in initiation/monitoring of DNA replication and development" Mutat Res 532(l-2):29-40 (2003)). The CDC7 kinase forms a complex with Dbf4, its regulatory subunit also known as ASK to generate an active Ser/Thr kinase. CDC7/Dbf4 kinase activity is required for initiation of DNA replication and subsequent transition into S- phase of the cell cycle. A second activator protein of CDC7 called Drfl or ASKLl has been identified in human cells, and appears to be involved in both S and M phase progression (Montagnoli et al., "Drfl, a novel regulatory subunit for human CDC7 kinase" EMBO J 21(12):3171-81 (2002); Yoshizawa-Sugata, "A second human Dbf4/ASK-related protein, Drfl /ASKLl, is required for efficient progression of S and M phases" Biol Chem 280(13): 13062-70 (2005)). CDC7 knock-out mice are embryonic lethal between E3.5 and E6.5 (Kim et al., "Inactivation of CDC7 kinase in mouse ES cells results in S-phase arrest and p53-dependent cell death" EMBO J 21(9):2168-79 (2002)). However, the analysis of conditional CDC7 as well as conditional Dbf4 knock-out ES cell lines revealed the essential roles of both proteins in mammalian cell proliferation and DNA synthesis (Kim et al., "Hypomorphic mutation in an essential cell-cycle kinase causes growth retardation and impaired spermatogenesis" EMBO J 22(19):5260-72 (2003); Yamashita et al, "Functional analyses of mouse ASK, an activation subunit for CDC7 kinase, using conditional ASK knockout ES cells" Genes Cells 10(6):551-63 (2005)).
[0008] Recently, CDC7 has emerged as an attractive target for cancer therapy. Depletion of CDC7 using siRNA oligonucleotides results in induction of apoptosis in cancer cell lines while normal dermal fibroblast cells are spared) Montagnoli et al., Cancer Res 64, 7110 (2004)). Further, CDC7 mediated phosphorylation sites on MCM2, MCM4 and MCM6 in tumor cells have been identified, but the functional relevance of those sites remains to be determined (Montagnoli et al., J of Biol Chem 281 :10281 (2006); Tsuji et al., MoI Biol Cell 17:4459-4472 (2006); Masai et al., J Biol Chem 281 :39249-39261 (2006); Sheu et al., MoI Cell 24:101-113 (2006)). There is evidence that the CDC7/Dbf4 complex is a target of the S checkpoint response to genotoxic stress. In HU-treated S. cerevisiae, Rad53 phosphorylates Dbf4 resulting in a removal of the kinase complex from chromatin and in inhibition of CDC7/Dbf4 kinase activity. Deletion of CDC7 results in HU hypersensitivity (Weinreich M and Stillman B, 1999). Further, Xenopus egg extracts treated with Etoposide, a Topoisomerase II inhibitor used in the clinic as anti-cancer agent, resulted in activation of a DNA damage checkpoint that required ATR, blocking CDC7/Dbf4 kinase activity (Costanzo 2003). This is contrary to recent data indicating that the CDC7/Dbf4 kinase is active during replication stress and contributes to hyper- phosphorylation of MCM2 in response to HU and Etoposide treatment (Tenca P et al., 2007). Further depletion of CDC7 using siRNA in the presence of those drugs increased cell death.
[0009] Accordingly, there is a need for potent and specific inhibitors of PIM, CDC7 or CK2, or any combination thereof. There is also a need for methods of treating PIM, CDC7, or CK2 mediated diseases, such as cancer, are also needed
SUMMARY OF THE INVENTION
[0010] This disclosure relates to compounds and pharmaceutical compositions of the compounds for inhibiting protein kinases such as PIM (PIM-I, PIM-2 and/or PIM- 3), CDC7 or CK2. [0011] One aspect of this dislcosure relates to compounds exemplified by Formula I as described herein.
[0012] Another aspect of this dislcosure relates to a pharmaceutical composition, comprising a compound according to Formula I and a pharmaceutically acceptable carrier, excipient, or diluent. [0013] Another aspect of this dislcosure relates to a method of inhibiting PIM, CDC7 or CK2 in a cell, comprising contacting the cell, in which inhibition of PIM, CDC7 or CK2 is desired, with a compound according to Formula I. [0014] Another aspect of this dislcosure relates to a method of inhibiting PIM, CDC7 or CK2 in a cell, comprising contacting a cell in which inhibition of PIM is desired with a pharmaceutical composition comprising a compound according to Formula I and a pharmaceutically acceptable carrier, excipient, or diluent. [0015] Another aspect of this dislcosure relates to a method of treating a disease or condition that involves PIM, CDC7 or CK2, comprising administering to a patient, in need of said treatment, a compound according to Formula I. [0016] Another aspect of this dislcosure relates to a method of treating a disease or condition that involves PIM, CDC7 or CK2, comprising administering to a patient, in need of said treatment, a pharmaceutical composition comprising a compound according to Formula I and a pharmaceutically acceptable carrier, excipient, or diluent. The disease or condition that can be treated by the compounds of Formula I, and the pharmaceutical compositions thereof, include cancer. Non- limiting examples of the types of cancer that can be treated include ovarian cancer, pancreatic cancer, prostate cancer, hepatocellular carcinoma, lymphomas, leukemias, cervical cancer, breast cancer (including breast carcinoma), colorectal cancer (including colorectal carcinoma), malignant melanoma, non-small cell lung cancer (NSCL) or glioblastomas. In another embodiment, the disease or condition that can be treated by the compound of formula I includes pancreatic cancer, prostate cancer, hepatocellular carcinoma, lymphomas, leukemias, colorectal cancer, breast carcinoma, colorectal carcinoma, malignant melanoma, and non-small cell lung cancer. In another embodiment, the disease or condition that can be treated by the compound of formula I includes breast carcinoma, colorectal carcinoma, malignant melanoma, and non- small cell lung cancer (NSCL). [0017] There are many different aspects of the compounds, pharmaceutical compositions thereof, and methods of use thereof, as described hereinbelow, and each aspect is non-limiting in regard to the scope of the invention. The transitional term "comprising" as used herein, which is synonymous with "including," "containing," or "characterized by," is inclusive or open-ended and does not exclude additional, unrecited elements or method steps.
[0018] The foregoing only summarizes certain aspects of this dislcosure and is not intended to be limiting in nature. These aspects and other aspects and embodiments are described more fully below.
DETAILED DESCRIPTION OF THE INVENTION [0019] There are many different aspects of the disclosure described hereinbelow, and each aspect is non-limiting in regard to the scope of the disclosure. The terms "aspects" and "embodiments" are meant to be non-limiting regardless of where the terms "aspect" or "embodiment" appears in this specification. The transitional term "comprising" as used herein, which is synonymous with "including," "containing," or "characterized by," is inclusive or open-ended and does not exclude additional, unrecited elements or methods. [0020] Aspect (A) of this disclosure relates to a compound according to formula I:
Figure imgf000006_0001
or a pharmaceutically acceptable salt thereof; wherein: R1 is hydrogen or alkyl;
R2 is selected from aminocarbonylalkylaminoalkyl, aminoalkylaminoalkyl, dialkylaminoalkylaminoalkyl, carboxyalkylaminoalkyl, cycloakylaminoalkyl, dialkylaminoarylalkylaminoalkyl, heteroarylalkylaminoalkyl, arylalkyl optionally substituted at any aryl or alkyl position with 1-3 groups selected from halo and -NH2, heterocycloalkylarylalkylaminoalkyl optionally substituted at the heterocycloalkyl portion with alkyl, aminoalkyl optionally substituted with 1 , 2 or 3 -OH, alkylamino optionally substituted with 1 , 2 or 3 -OH, alkylaminoalkyl optionally substituted with 1, 2 or 3 -OH, alkoxyalkylaminoalkyl, heterocycloalkylaminoalkyl optionally substituted with alkyl at the heterocycloalkyl portion, hydroxyalkyl, cycloalkylaminoalkyl, arylamino(alkyl)alkyl optionally substituted at any ring position with 1, 2 or 3 halo, heterocycloalkylalkylaminoalkyl optionally substituted at any ring position with 1-2 alkyl, arylalkylaminoalkyl optionally substituted at the aryl position with dialkylamino, halo, alkoxy, heteroaryl, or alkylheterocycloalkyl, dialkylaminoalkyl optionally substituted with 1 , 2 or 3 -OH, arylaminoalkyl optionally substituted at the aryl portion with 1, 2 or 3 groups selected from halo, heterocycloalkyl, alkylheterocycloalkyl and dialkylamino, heteroarylaminoalkyl, arylamino, aryloxyalkyl, unsubstituted heteroarylalkyl, heteroarylalkyl substituted at any alkyl position with alkyl, aryl, arylalkyl or amino, -(C4-Cy)cycloalkyl optionally substituted with -NH2, -NHC(O)-O-(CH3)3, or aminoalkyl, -N(H)C(O)-O-alkyl, alkylpiperazinylcarbonyl, alkylheterocycloarylalkoxyalkyl, heteroaryl optionally substituted at any ring position with 1, 2 or 3 substituents selected from amino, alkyl, alkylamino, halo, -O-heterocycloalkyl, alkoxy, aminoalkyl, dialkylaminoalkylamino, heterocycloalkylalkylamino and heterocycloalkyl, heterocycloalkylalkylamino, heterocycloalkylalkyl optionally substituted with 1 , 2 or 3 R4 groups at any ring position, aryl substituted with 1, 2 or 3 R5 groups at any ring position, -NHC(O)R7, aminoalkylamino, -CR11R12, heterocycloalkyl optionally substituted with 1, 2 or 3 R10 groups, heteroarylalkyl substituted at any ring position with 1, 2 or 3 alkyl, halo, aryl or arylalkyl groups, heteroarylamino, heterocycloalkylalkoxyalkyl, dialkylaminoalkylamino, heterocycloalkylamino, carboxyalkyl, arylalkylamino optionally substituted with heterocycloalkyl or heterocycloalkylalkyl, and heterocycloalkyloxyalkyl; or R1 and R2, together with the carbon atoms to which they are attached, join to form a five membered heterocycloalkyl ring;
R3a is selected from halo, alkyl, -NO2, alkoxy, alkynyl optionally substituted with R14, alkoxycarbonylalkyl, arylalkoxy, -C(O)N(H)alkyl, -N(H)-C(O)-alkyl, - C(O)-alkyl,
-CN, phenyl, -OCF3, -N(H)R13, -OH, -CF3, -S-CH3 and hydroxymethylalkynyl;
R3b, R3c and R3d are each independently selected from H, -OH, -N+(O)OH, alkoxyl, and halo; or R3a is hydrogen and R3b, R3c and R3d are each independently selected from -CF3, -OH, alkoxy, and halo; or R3a and R3d, together with the carbons to which they are attached, join to form a 5 membered heteroaryl optionally substituted with methyl or -NH2, or a 5-6 membered heterocycloalkyl;
R4 is selected from -OH, amino, aminoalkyl, halo, alkyl optionally substituted with -OH, alkoxy, alkylaminoalkyl, heteroarylalkyl, -C(O)OH, -C(O)-O-alkyl, -C(O)- alkyl, oxo, aryl optionally substituted with alkyl, arylalkyl or halo, heteroaryl, -OH, dialkylamino, dialkylaminoalkyl, alkylamino, spiro-heterocycloalkyl, -NHC(O)R8, -C(O)NHR9, arylalkylaminocarbonyl optionally substituted with halo at any ring position of the aryl, heterocycloalkylalkylamino, dialkylaminoalkylcarbonyl, dialkylaminocarbonylalkyl, heterocycloalkylalkyl optionally substituted with -CF3, heterocycloalkyl optionally substituted with alkyl, arylalkyl optionally substituted with
-CF3, alkoxyalkyl and heterocycloalkylcarbonyl optionally substituted with -OH or halo; R5 is selected from alkyl, -OH, amino, aminoalkyl, -C(O)N(H)- heteroarylalkyl, halo, -NO2, -C(O)-N(H)-heterocycloalkylalkyl, alkylaminoalkyl, heteroaryl, cycloalkylaminoalkyl, alkylamino, dialkylamino, -C(O)Oalkyl, -C(O)OH, heterocycloalkyl, -N(H)-alkylheterocycloalkylC(O)-O-alkyl, -O-alkyl-C(O)-N(H)- alkylcycloalkyl, -C(O)-N(H)-alkyl, -C(O)N(H)alkylaryl, -C(O)N(H)-cycloalkyl, alkylthio, alkylsulfonyl, -O-alkylheterocycloalkyl, heteroarylalkylamino, -CF3, heterocycloalkylalkylamino optionally substituted with alkyl at any ring position, alkylsulfonyl, -NHC(O)R6, alkoxycarbonylheterocycloalkylaminoalkyl, heterocycloalkylaminoalkyl optionally substituted at the heterocycloalkyl portion with alkoxycarbonyl, dialkylaminoalkyl, dialkylaminoalkylamino, and alkoxy;
R6 is selected from dialkylaminoalkyl, heteroarylamino, heterocycloalkyl, heterocycloalkylalkyl optionally substituted with -OH, cycloalkyl, heteroarylalkyl, alkoxyalkyl, heterocycloalkyl, heteroaryl optionally substituted with 1 , 2 or 3 groups selected from halo, -NH2, aminoalkylaminocarbonyl, heteroaryl, hydroxyalkyl, alkoxy, alkyl, -C(O)-O-alkyl and -C(O)-O-H, alkyl, alkoxy, and aryl optionally substituted 1, 2 or 3 halo, -N(H)C(O)CH3, alkyl or alkoxy;
R7 is selected from heterocycloalkylalkyl, arylalkyl optionally substituted at any ring position with 1, 2 or 3 halo groups, dialkylaminoalkyl, heterocycloalkyl, heteroaryl optionally substituted at any ring position with 1 , 2 or 3 groups selected from halo and -COOH, and alkoxyalkyl;
R8 is selected from arylalkyl, heterocycloalkyl and alkyl; R9 is selected from H, alkyl, arylalkyl optionally substituted with halo at any ring position of the aryl, heterocycloalkyl, arylalkylaminocarbonyl optionally substituted with halo and dialkylaminoalkyl;
R10 is selected from alkyl, oxo, heteroaryl, dialkylaminoalkylcarbonyl, dialkylaminocarbonylalkyl, aminoalkyl, -OH, halo, heteroarylcarbonyl, dialkylaminoalkyl, heterocycloalkyl optionally substituted with alkyl,
-C(O)-O-alkyl, arylalkylcarbonyl, arylcarbonyl, alkylcarbonyl, alkoxyalkylcarbonyl, heterocycloalkylcarbonyl, heteroarylalkyl, -O-heterocycloalkyl and arylalkyl optionally substituted with alkoxy or arylalkoxy;
R11 is selected from aryl optionally substituted with halo, heteroarylalkyl, cycloalkyl, spiro-cycloalkyl and arylalkyl optionally substituted with alkoxy or phenylmethylmethoxy;
R12 is selected from -NH2 and heterocycloalkyl optionally substituted with alkyl;
R13 is selected from arylalkyl wherein the aryl portion of arylalkyl is optionally substituted with 1, 2 or 3 alkoxy, halo, methyl, methoxy, -CF3, cycloalkyl (such as cyclohexyl), and heteroarylalkyl (such as pyridinealkyl); and
R14 is selected from hydroxylalkyl, H and TMS. [0021] In another embodiment of formula I, R3a is selected from halo, alkyl, - NO2, alkoxy, alkynyl optionally substituted with R14, alkoxycarbonylalkyl, arylalkoxy,
-C(O)N(H)alkyl, -N(H)-C(O)-alkyl, -C(O)-alkyl, -CN, phenyl, -OCF3, -N(H)R13, - OH,
-CF3, -S-CH3 and hydroxymethylalkynyl; and
R3b, R3c and R3d are each H. [0022] In another embodiment of formula I, R3a is halo, alkoxy or -OCF3; and
R3b, R3c and R3d are each H. Aspect (B) of this disclosure relates to a compound of Formula I,
Figure imgf000010_0001
or a pharmaceutically acceptable salt thereof; wherein: R1 is hydrogen or alkyl; R2 is selected from aminocarbonylalkylaminoalkyl, aminoalkylaminoalkyl, dialkylaminoalkylaminoalkyl, carboxyalkylaminoalkyl, cycloakylaminoalkyl, dialkylaminoarylalkylaminoalkyl, heteroarylalkylaminoalkyl, arylalkyl, heterocycloalkylarylalkylaminoalkyl optionally substituted at the heterocycloalkyl portion with alkyl, alkoxyalkylaminoalkyl, heterocycloalkylaminoalkyl optionally substituted with alkyl at the heterocycloalkyl portion, hydroxyalkyl, cycloalkylaminoalkyl, arylamino(alkyl)alkyl optionally substituted at any ring position with 1, 2 or 3 halo, heterocycloalkylalkylaminoalkyl optionally substituted at any ring position with 1-2 alkyl, arylalkylaminoalkyl optionally substituted at the aryl position with dialkylamino, halo, alkoxy, heteroaryl, or alkylheterocycloalkyl, arylamino alkyl optionally substituted at the aryl portion with 1, 2 or 3 groups selected from halo, alkylheterocycloalkyl and dialkylamino, heteroarylaminoalkyl, arylamino, aryloxyalkyl, heteroarylalkyl optionally substituted with arylalkyl, alkyl or aryl, - N(H)C(O)-O-alkyl, alkylpiperazinylcarbonyl, alkylheterocycloarylalkoxyalkyl, heteroaryl optionally substituted at any ring position with 1 , 2 or 3 substituents selected from -O-heterocycloalkyl, dialkylaminoalkylamino and heterocycloalkylalkylamino, heterocycloalkylalkylamino, heterocycloalkylalkyl optionally substituted with 1 , 2 or 3 R4 groups at any ring position, aryl substituted with 1, 2 or 3 R5 groups at any ring position, -NHC(O)R7, aminoalkylamino, - CR11R12, heterocycloalkyl optionally substituted with 1, 2 or 3 R10 groups, heteroarylalkyl optionally substituted at any ring position with 1 , 2 or 3 alkyl or aryl groups, heteroarylamino, heterocycloalkylalkoxyalkyl, dialkylaminoalkylamino, heterocycloalkylamino, carboxyalkyl, and heterocycloalkyloxyalkyl; or R1 and R2, together with the carbon atoms to which they are attached, join to form a five membered heterocycloalkyl ring; R3a is selected from halo, alkyl, -NO2, alkoxy, alkynyl optionally substituted with R14, alkoxycarbonylalkyl, arylalkoxy, -C(O)N(H)alkyl, -N(H)-C(O)-alkyl, - C(O)-alkyl, -CN, phenyl, -OCF3, -N(H)R13, -OH, -CF3, -S-CH3 and hydroxymethylalkynyl;
R3b, R3c and R3d are each independently selected from H, -OH, -N+(O)OH, alkoxyl, and halo; or R3a is hydrogen and R3b, R3c and R3d are each independently selected from -CF3, -OH, alkoxy, and halo; or R3a and R3d, together with the carbons to which they are attached, join to form a 5 membered heteroaryl optionally substituted with methyl or -NH2, or a 5-6 membered heterocycloalkyl;
R4 is selected from -OH, amino, aminoalkyl, halo, alkyl optionally substituted with -OH, alkoxy, alkylaminoalkyl, heteroarylalkyl, -C(O)OH, -C(O)-O-alkyl, -C(O)- alkyl, oxo, aryl optionally substituted with alkyl or halo, heteroaryl, -OH, dialkylamino, dialkylaminoalkyl, alkylamino, spiro-heterocycloalkyl, -NHC(O)R8, -C(O)NHR9, arylalkylaminocarbonyl optionally substituted with halo at any ring position of the aryl, heterocycloalkylalkylamino, dialkylaminoalkylcarbonyl, dialkylaminocarbonylalkyl, heterocycloalkylalkyl optionally substituted with -CF3, heterocycloalkyl optionally substituted with alkyl, alkoxyalkyl, arylalkyl optionally substituted with -CF3, and heterocycloalkylcarbonyl optionally substituted with -OH or halo;
R5 is selected from -C(O)N(H)-heteroarylalkyl, -C(O)-N(H)- heterocycloalkylalkyl, alkylaminoalkyl, cycloalkylaminoalkyl, -N(H)-alkylheterocycloalkylC(O)-O-alkyl, -O-alkyl-C(O)-N(H)-alkylcycloalkyl, -C(O)N(H)alkylaryl, -C(O)N(H)-cycloalkyl, -O-alkylheterocycloalkyl, heteroarylalkylamino, heterocycloalkylalkylamino optionally substituted with alkyl at any ring position, alkoxycarbonylheterocycloalkylaminoalkyl, heterocycloalkylaminoalkyl optionally substituted at the heterocycloalkyl portion with alkoxycarbonyl, dialkylaminoalkyl and dialkylaminoalkylamino;
R6 is selected from dialkylaminoalkyl, heteroarylamino, heterocycloalkyl, heterocycloalkylalkyl optionally substituted with -OH, cycloalkyl, heterocycloalkylalkyl, heteroarylalkyl, alkoxyalkyl, heterocycloalkyl, heteroaryl optionally substituted with 1, 2 or 3 groups selected from halo, -NH2, aminoalkylaminocarbonyl, heteroaryl, hydroxyalkyl, alkoxy, alkyl, -C(O)-O-alkyl and -C(O)-O-H, alkyl, alkoxy, and aryl optionally substituted 1, 2 or 3 halo, - N(H)C(O)CH3, alkyl or alkoxy;
R7 is selected from heterocycloalkylalkyl, arylalkyl optionally substituted at any ring position with 1, 2 or 3 halo groups, dialkylaminoalkyl, heterocycloalkyl, heteroaryl optionally substituted at any ring position with 1, 2 or 3 groups selected from halo and -COOH, and alkoxyalkyl;
R8 is selected from arylalkyl, heterocycloalkyl and alkyl;
R9 is selected from H, alkyl, arylalkyl optionally substituted with halo at any ring position of the aryl, heterocycloalkyl, arylalkylaminocarbonyl optionally substituted with halo and dialkylaminoalkyl;
R10 is selected from alkyl, oxo, heteroaryl, dialkylaminoalkylcarbonyl, dialkylaminocarbonylalkyl, aminoalkyl, -OH, halo, heteroarylcarbonyl, dialkylaminoalkyl, heterocycloalkyl (piperidinyl) optionally substituted with alkyl, - C(O)-O-alkyl, arylalkylcarbonyl, arylcarbonyl, alkylcarbonyl, alkoxyalkylcarbonyl, heterocycloalkylcarbonyl, heteroarylalkyl, -O-heterocycloalkyl and arylalkyl;
R11 is selected from aryl optionally substituted with halo, heteroarylalkyl, cycloalkyl, spiro-cycloalkyl and arylalkyl optionally substituted with alkoxy or phenylmethylmethoxy; R12 is selected from -NH2 and heterocycloalkyl optionally substituted with alkyl; R13 is selected from arylalkyl wherein the aryl portion of arylalkyl is optionally substituted with 1, 2 or 3 alkoxy, halo, methyl, methoxy, -CF3, cycloalkyl (such as cyclohexyl), and heteroarylalkyl (such as pyridinealkyl); and
R14 is selected from hydroxylalkyl, H and TMS. [0024] All of the compounds disclosed herein include either their free base form or their pharmaceutically acceptable salts whether it is stated in the specification that these compounds can exist as their pharmaceutically acceptable salt or not. So, for instance, for any given embodiment of the compound of Formula I (including embodiments relating to the compounds themselves or method of use thereof), this embodiment includes either its free base form or any of its pharmaceutically acceptable salts, whether this is stated within this embodiment or not. [0025] In another embodiment, R2 is -CH2-RiS, wherein Ri5 is selected from aminocarbonylalkylamino, dialkylaminoalkylamino, carboxyalkylamino, cycloakylamino, dialkylaminoarylalkylamino, heteroarylalkylamino, heterocycloalkylarylalkylaminol optionally substituted at the heterocycloalkyl portion with alkyl, amino, alkylamino optionally substituted with 1, 2 or 3 -OH, alkoxy alky lamino, heterocycloalkylamino optionally substituted with alkyl at the heterocycloalkyl portion, cycloalkylamino, arylamino(alkyl) optionally substituted at any ring position with 1, 2 or 3 halo, 4-(4-methylpiperazine-lyl) phenyl] methyloxy, heterocycloalkylalkylamino optionally substituted at any ring position with alkyl, arylalkylamino optionally substituted at the aryl position with dialkylamino, halo, alkoxy, heteroaryl, or alkylheterocycloalkyl, dialkylamino optionally substituted with 1 , 2 or 3 -OH, and arylamino optionally substituted at the aryl portion with 1 , 2 or 3 groups selected from halo, alkylheterocycloalkyl and dialkylamino. [0026] In another embodiment of formula I, R3a is halo, alkyl, -NO2, alkoxy, alkynyl optionally substituted with R14, alkoxycarbonylalkyl, arylalkoxy, - C(O)N(H)alkyl,
-N(H)-C(O)-alkyl, -C(O)-alkyl, -CN, phenyl, -OCF3, -N(H)R13, -OH, -CF3, -S-CH3 and hydroxymethylalkynyl; and R3b, R3c and R3d are each H.
[0027] In another embodiment of formula I, R3a is halo, alkoxy or -OCF3; and R3b, R3c and R3d are each H. [0028] In other embodiments of aspect (A) (and aspect (B) where it can apply) described above, R2 in formula I is a heterocycloalkyl selected from azetidinyl, pyrrolidinyl, piperazinyl and piperidinyl optionally substituted with 1, 2 or 3 R10. [0029] In other embodiments of aspect (A) (and aspect (B) where it can apply) described above, R2 in formula I is a heterocycloalkyl selected from azetidinyl, pyrrolidinyl, piperazinyl and piperidinyl optionally substituted with 1, 2 or 3 R10. [0030] In other embodiments of aspect (A) (and aspect (B) where it can apply) described above, R2 in formula I is a heterocycloalkyl selected from azetidinyl, pyrrolidinyl, piperazinyl and piperidinyl optionally substituted with 1, 2 or 3 R10; R3a is halo, alkoxy or -OCF3; and R3b, R3c and R3d are each H.
[0031] In other embodiments of aspect (A) (and aspect (B) where it can apply) described above, R2 in formula I is a heteroaryl optionally substituted at any ring position with 1, 2 or 3 substituents selected from amino, alkylamino, halo, -O-heterocycloalkyl, alkoxy, aminoalkyl, dialkylaminoalkylamino, heterocycloalkylalkylamino and heterocycloalkyl.
[0032] In other embodiments of aspect (A) (and aspect (B) where it can apply) described above, R2 in formula I is a heteroaryl optionally substituted at any ring position with 1, 2 or 3 substituents selected from amino, alkylamino, halo, -O-heterocycloalkyl, alkoxy, aminoalkyl, dialkylaminoalkylamino, heterocycloalkylalkylamino and heterocycloalkyl; R3a is halo, alkoxy or -OCF3; and R3b, R3c and R3d are each H.
[0033] In other embodiments of aspect (A) (and aspect (B) where it can apply) described above, R2 in formula I is a heterocycloalkylalkyl optionally substituted with 1 , 2 or 3 R4 groups at any ring position. [0034] In other embodiments of aspect (A) (and aspect (B) where it can apply) described above, R1 in formula I is H.
[0035] In other embodiments of aspect (A) (and aspect (B) where it can apply) described above, R2 in formula I is a heterocycloalkylalkyl optionally substituted with 1, 2 or 3 R4 groups at any ring position; R3a is halo, alkoxy or -OCF3; and R3b, R3c and R3d are each H.
[0036] In other embodiments of aspect (A) (and aspect (B) where it can apply) described above, R2 in formula I is an aryl substituted with 1, 2 or 3 R5 groups at any ring position. [0037] In other embodiments of aspect (A) (and aspect (B) where it can apply) described above, R2 in formula I is an aryl substituted with 1, 2 or 3 R5 groups at any ring position; R3a is halo, alkoxy or -OCF3; and R3b, R3c and R3d are each H. [0038] In other embodiments of aspect (A) (and aspect (B) where it can apply) described above, R2 in formula I is a heterocycloalkyl optionally substituted with 1, 2 or 3 R10 groups.
[0039] In other embodiments of aspect (A) (and aspect (B) where it can apply) described above, R2 in formula I is a heterocycloalkyl optionally substituted with 1, 2 or 3 R10 groups; R3a is halo, alkoxy or -OCF3; and R3b, R3c and R3d are each H. [0040] In other embodiments of aspect (A) (and aspect (B) where it can apply) described above, R2 in formula I is a heteroarylalkyl optionally substituted at any ring position with 1, 2 or 3 alkyl or aryl groups.
[0041] In other embodiments of aspect (A) (and aspect (B) where it can apply) described above, R2 in formula I is a heteroarylalkyl optionally substituted at any ring position with 1 2 or 3 alkyl or aryl groups; R3a is halo, alkoxy or -OCF3; and R3b, R3c and R3d are each H.
[0042] In other embodiments of aspect (A) (and aspect (B) where it can apply) described above, R7 in formula I is heteroaryl, such as pyridinyl or imidazolyl, which can be optionally substituted as described above in formula I. [0043] In other embodiments of aspect (A) (and aspect (B) where it can apply) described above, R2 in formula I is heterocycloalkyl, such as morpholinyl, piperidinyl, pyrrolidinyl, piperazinyl or tetrahydrofuan, which can be optionally substituted as described above in formula I. [0044] In other embodiments of aspect (A) (and aspect (B) where it can apply) described above, R2 in formula I is heterocycloalkylaminoalkyl, wherein the heterocycloalkyl potion is piperidinyl, piperazinyl, pyrrolidinyl or imidazolyl, which can be optionally substituted as described above in formula I. [0045] In other embodiments of aspect (A) (and aspect (B) where it can apply) described above, R2 in formula I is heterocycloalkylalkyl, wherein the heterocycloalkyl potion is morpholinyl, piperidinyl, pyrrolidinyl, piperazinyl or tetrahydrofuanyl, which can be optionally substituted as described above in formula I. [0046] In other embodiments of aspect (A) (and aspect (B) where it can apply) described above, R2 in formula I is alkylheterocycloalkyl, wherein the heterocycloalkyl potion is piperizinyl, which can be optionally substituted as described above in formula I.
[0047] In other embodiments of aspect (A) (and aspect (B) where it can apply) described above, R2 in formula I is heteroaryl, such as thienyl, furan, pyrazol, thiazol, isoxazol, tetrahydroisoquinolinyl and imidazol, which can be optionally substituted as described above in formula I.
[0048] In other embodiments of aspect (A) (and aspect (B) where it can apply) described above, R2 in formula I is phenyl which is substituted as described above in formula I. [0049] In other embodiments of aspect (A) (and aspect (B) where it can apply) described above, R6 is heteroaryl, such as imidazol, oxazole, pyridine, pyrimidine, isoxazole or furanyl, which can be optionally substituted as described above in formula I.
[0050] In other embodiments of aspect (A) (and aspect (B) where it can apply) described above, R3a is Br, Cl or -OCH3, and R3b, R3c and R3d are each H.
[0051] In other embodiments of aspect (A) (and aspect (B) where it can apply) described above, R1 is H.
[0052] In other embodiments of aspect (A) (and aspect (B) where it can apply) described above, R2 is heterocycloalkyl, such as piperidinyl, piperazinyl, pyrrolidinyl and morpholinyl, which can be optionally substituted as described above in formula I.
[0053] In other embodiments of aspect (A) (and aspect (B) where it can apply), R2 is selected from -(Ci-C3)alkyl-phenyl optionally substituted with 1-3 halo, -NH- phenyl,
-NH-piperidinyl, -NH-pyridinyl, -NH(Ci-C3)alkylphenyl optionally substituted at any phenyl position with piperazinyl or methylpiperazinyl, -NH(Ci-C3)alkyl-N(CH3)2,
-NH(Ci-C3)alkyl-OH, phenyl substituted with 1, 2 or 3 Xa, phenyl substituted with
0-2 Xb and 1 Xc group, methylpiperazinylphenylalkoxyalkyl
(methylpiperazinylphenylmethoxymethyl), methylpiperazinylcarbonyl, 2- chlorophenyl-4-methylpiperazinylmethyl, (4-methylpiperazin- 1 -yl)(phenyl)methyl, 1 - (4-methylpiperazin- 1 -yl)-2-phenylethyl, 2-chlorophenyl(4-methylpiperazin- 1 - yl)methyl, 4-oxo-3-phenyl-l,3,8-triazaspiro[4.5]dec-l-yl)methyl, -(Ci-
C3)alkylC(O)OH, hydroxyalkyl, -(Ci-C3)alkyl-N(Rza)-aryl optionally substituted with chloro, fluoro, piperazinyl, methylpiperazinyl and dialkylamino, -(5-10)membered heteroaryl optionally substituted with 1 or 2 groups selected from halo, -(Ci-Ce)alkyl, -(Ci-Ce)alkoxy, piperidinylalkylamino, piperdinylalkylamino, alkylamino, aminoalkyl, dialkylaminoalkylamino, piperidinyloxy, piperidinyl, and amino, -(Ci-C3)alkyl-O-phenyl, -(Ci-C3)alkyl-O-(Ci-C3)alkyl-(5-6 membered)heterocycloalkyl, -(Ci-C3)alkyl-N(H)-heteroaryl, -(Ci-C3)alkyl-(5- 10)membered heteroaryl optionally substituted with -(Ci-C3)alkyl, halo or phenyl, oxopyrrolidinyl optionally substituted with OH or piperidinyl, -(Ci-C4)alkyl-(3-9 membered)heterocycloalkyl optionally substituted at the (3-9 membered)heterocycloalkyl with Xd, -(Ci-C6)alkyl-NRzb-(Ci-C4)alkyl wherein the - (Ci-C4)alkyl portion is optionally substituted with Xe, -(Ci-C3)alkyl-NH-(C3- C6)cycloalkyl, -(CH2)-NH-(C3-C6)cyclohexyl, -(CrC3)alkyl-NH2, wherein the -(C1- C3)alkyl- portion of -(Ci-C3)alkyl-NH2 is optionally substituted with Xf, -(3-9 membered)heterocycloalkyl optionally substituted with Xg, -(C3-C6)-cycloalkyl (cyclohexyl) optionally substituted with amino, -NHC(O)-O-(CH3)3, aminoalkyl and dialkylaminoalkylamino;
Xa is selected from halo, phenyl substituted with a group selected from - COOH, -COOCH3, NO2, -(Ci-C3)alkoxy, methylthio, -(Ci-C3)alkyl, -NH2, -OH, -Nt(C1- C3)alkyl]2, -CF3 and methylsulfonyl;
Xb, when present, is independently selected from alkyl, -NH2 and halo; Xc is selected from -(5-6 membered)heteroaryl (imidazole), -(5-6 membered)heterocycloalkyl (piperazinyl), alkylcarbonylamino, alkylaminocarbonyl, cycloalkylaminoalkyl (cyclohexylaminoalkyl), -NH(Ci-C3)alkyl-(3-6 membered)heterocycloalkyl, dimethylamino-(Ci-C3)alkylcarbonylamino, cycloalkylaminocarbonyl, dialkylaminoalkylcarbonylamino, cycloalkylmethylaminocarbonylmethyloxy, phenylalkylaminocarbonyl, heterocycloalkylaminoalkyl optionally substituted with alkoxycarbonyl, dialkylaminoalkyl, morpholinylalkoxy, alkylaminoalkyl, dialkylaminoalkylamino, alkoxycarbonylheterocycloalkylalkylamino, heterocycloalkylalkylamino optionally substituted with methyl, heterocycloalkylalkylcarbonylamino optionally substituted with -OH, , heterocycloalkylcarbonylamino optionally substituted with 1 or 2 groups selected from halo and methyl, heteroarylcarbonylamino optionally substituted with 1 or 2 groups selected from amino, alkyl, halo, -C(O)OH, pyrazolyl, -OCH3 and -
C(O)OCH3,
-N(H)C (O)phenyl optionally substituted with 1 or 2 groups selected from halo, methyl, methoxy and -NHC(O)CH3, -N(H)C(O)alkyl, -N(H)C(O)(C1- C3)alkylpyridinyl,
-N(H)C(O)(Ci-C3)alkylpiperidinyl, -N(H)C(O)-lH-pyrrolo[2,3-b]pyridinyl,
-N(H)C(O)cyclohexyl, N(H)C(O)cyclopentyl, -N(H)C(O)(C i-C3)alkylmorpholinyl,
-N(H)C(O)(Ci-C3)alkylpyridinyl, -N(H)C(O)(Ci-C3)alkylimidazolyl, aminoalkyl, and
-N(H)C(O)N(H)pyrimidinyl; Xd is selected from alkyl, 1-3 halo, -COOH, phenyl optionally substituted with 1 or 2 groups selected from halo, methyl and methylphenyl, phenylmethyl, spiro- piperidine, trifluoromethylphenylmethyl, -(Ci-C3)alkoxy, pyridinyl, dimethylaminoalkyl, dimethylamino, hydroxylalkyl, dimethylaminoalkylaminocarbonyl, alkylamino, aminoalkyl, dimethylaminocarbonylalkyl, diethylaminoalkylcarbonyl, -(Ci-C3)alkyl-(5-6 membered)heterocycloalkyl, (5-6 membered)heterocycloalkyl optionally substituted with -(Ci-C3)alkyl, -NH2, -OH, heterocycloalkylalkylamino, alkoxyalkyl,
-C(O)CH3, -C(O)NH(C i-C3)alkylphenyl optionally substituted with 1-3 halo at any phenyl position, one -OH and one -C(O)NH(C i-C3)alkylphenyl optionally substituted with 1-3 halo at any phenyl position, -C(O)-heterocycloalkyl optionally substituted with
-OH or halo, alkoxycarbonyl, aminocarbonyl, one -OH and one methyl, one -OH and one -C(O)OH, one -OH and one -NHC(O)piperidinyl, one -OH and one alkyl;
Xe is selected from dialkylamino, amino, 1-3 -OH, alkoxy, 4- methylpiperazinylphenyl, dimethylaminophenyl, phenyl optionally substituted with 1-
3 groups selected from halo and methoxy, heteroaryl, -(Ci-C3)alkylC(O)NH2, -
C(O)NH2,
-C(O)OH, -(Ci-C3)alkylC(O)OH, heterocycloalkyl optionally substituted with 1-2 alkyl; Xf is selected from cycloalkyl, spirocycloalkyl, phenyl, phenylalkyl optionally substituted with phenylmethyloxy or alkoxy and thienylalkyl;
Xg is selected from alkyl, alkylcarbonyl, heterocycloalkylcarbonyl, dialkylaminoalkylcarbonyl, 1 -methylpiperidinyl, dialkylaminoalkyl, heteroarylcarbonyl, alkoxyalkylcarbonyl, phenylcarbonyl, phenylalkylcarbonyl, oxo, phenylalkyl, -(5-6 membered)heteroarylalkyl, piperidinyloxy, -OH, oxo, 1-2 halo and 1-2 methyl;
Rza is H or methyl; and Rzb is H or alkyl optionally substituted with 1-3 -OH.
In another embodiment, R2 is selected -NH-phenyl, -NH-piperidinyl, -NH- pyridinyl, -NH(Ci-C3)alkylphenyl optionally substituted at any phenyl position with piperazinyl or methylpiperazinyl, -NH(Ci-C3)alkyl-N(CH3)2, -NH(d-C3)alkyl-OH, - (Ci-C3)alkyl-O-phenyl, -(Ci-C3)alkyl-O-(Ci-C3)alkyl-(5-6 membered)heterocycloalkyl, -(Ci-C3)alkyl-N(H)-heteroaryl, -(Ci-C3)alkyl-(5-
10)membered heteroaryl optionally substituted with -(Ci-C3)alkyl, halo or phenyl, oxopyrrolidinyl optionally substituted with OH or piperidinyl, -(d-C4)alkyl-(3-9 membered)heterocycloalkyl optionally substituted at the (3-9 membered)heterocycloalkyl with Xd, -(Ci-C6)alkyl-NRzb-(Ci-C4)alkyl wherein the - (Ci-C4)alkyl portion is substituted with Xe, -(Ci-C3)alkyl-NH-(C3-C6)cycloalkyl, - (CH2)-NH-(C3-C6)cyclohexyl, -(d-C3)alkyl-NH2, wherein the -(CrC3)alkyl- portion of -(Ci-C3)alkyl-NH2 is substituted with Xf, and -(3-9 membered)heterocycloalkyl optionally substituted with Xg;
Xd is selected from alkyl, 1-3 halo, -COOH, phenyl optionally substituted with 1 or 2 groups selected from halo, methyl and methylphenyl, phenylmethyl, spiro- piperidine, trifluoromethylphenylmethyl, -(Ci-C3)alkoxy, pyridinyl, dimethylaminoalkyl, dimethylamino, hydroxylalkyl, dimethylaminoalkylaminocarbonyl, alkylamino, aminoalkyl, dimethylaminocarbonylalkyl, diethylaminoalkylcarbonyl, -(C i -C3)alkyl-(5 -6 membered)heterocycloalkyl, (5-6 membered)heterocycloalkyl optionally substituted with -(Ci-C3)alkyl, -NH2, -OH, heterocycloalkylalkylamino, alkoxyalkyl, -C(O)CH3, -C(O)NH(C i-C3)alkylphenyl optionally substituted with 1-3 halo at any phenyl position, one -OH and one -C(O)NH(C i-C3)alkylphenyl optionally substituted with 1-3 halo at any phenyl position, -C(O)-heterocycloalkyl optionally substituted with
-OH or halo, alkoxycarbonyl, aminocarbonyl, one -OH and one methyl, one -OH and one -C(O)OH, one -OH and one -NHC(O)piperidinyl, one -OH and one alkyl; Xe is selected from dialkylamino, amino, 1-3 -OH, alkoxy, A- methylpiperazinylphenyl, dimethylaminophenyl, phenyl optionally substituted with 1- 3 groups selected from halo and methoxy, heteroaryl, -(Ci-C3)alkylC(O)NH2, - C(O)NH2, -C(O)OH, -(Ci-C3)alkylC(O)OH, heterocycloalkyl optionally substituted with 1-2 alkyl;
Xf is selected from cycloalkyl, spirocycloalkyl, phenyl, phenylalkyl optionally substituted with phenylmethyloxy or alkoxy and thienylalkyl;
Xg is selected from alkyl, alkylcarbonyl, heterocycloalkylcarbonyl, dialkylaminoalkylcarbonyl, 1-methylpiperidinyl, dialkylaminoalkyl, heteroarylcarbonyl, alkoxyalkylcarbonyl, phenylcarbonyl, phenylalkylcarbonyl, oxo, phenylalkyl, -(5-6 membered)heteroarylalkyl, piperidinyloxy, -OH, oxo, 1-2 halo and 1-2 methyl; and
Rzb is H or alkyl optionally substituted with 1-3 -OH. [0054] In other embodiments of Aspect A of this disclosure (and Aspect B where it applies), R2 can be any one of the following (22) embodiments for R2: [0055] In another embodiment (1), R2 is -(Ci-C3)alkyl-phenyl optionally substituted with 1-3 halo. [0056] In another embodiment (2), R2 is selected from -NH-phenyl, -NH- piperidinyl, -NH-pyridinyl, -NH(Ci-C3)alkylphenyl optionally substituted at any phenyl position with piperazinyl or methylpiperazinyl, -NH(Ci-C3)alkyl-N(CH3)2 and -NH(Ci-C3)alkyl- OH. [0057] In another embodiment (3), R2 is phenyl substituted with 1-3 Xa groups, wherein Xa is selected from halo, -COOH, -COOCH3, NO2, -(CrC3)alkoxy, methylthio, -(Ci-C3)alkyl, -NH2, -OH, -N[(Ci-C3)alkyl]2, -CF3 and methylsulfonyl. [0058] In another embodiment (4), R2 is phenyl substituted with 0-2 Xb and 1 Xc group, wherein Xb, when present, is selected from alkyl, -NH2 and halo, and Xc is selected from -(5-6 membered)heteroaryl (such as, for example, imidazole), -(5-6 membered)heterocycloalkyl (such as, for example, piperazinyl), alkylcarbonylamino, alkylaminocarbonyl, cycloalkylaminoalkyl (such as, for example, cyclohexylaminoalkyl), -NH(Ci-C3)alkyl-(3-6 membered)heterocycloalkyl, dimethylamino-(Ci-C3)alkylcarbonylamino, cycloalkylaminocarbonyl, dialkylaminoalkylcarbonylamino, cycloalkylmethylaminocarbonylmethyloxy, phenylalkylaminocarbonyl, heterocycloalkylaminoalkyl optionally substituted with alkoxycarbonyl (such as, for example, piperidinylaminomethyl or methylpiperdinylaminomethyl), heterocycloalkylalkylaminoalkyl (such as, for example, piperidinylethylaminomethyl), dialkylaminoalkyl, morpholinylalkoxy, alkylaminoalkyl, dialkylaminoalkylamino (such as, for example, dimethylamino(Ci- C5)alkylamino such as 3-(dimethylamino)-2,2-dimethylpropyl]amino), alkoxycarbonylheterocycloalkylalkylamino, heterocycloalkylalkylamino optionally substituted with methyl (such as, for example, piperidinylmethylamino, piperidinylethylamino, tetrahydrofuranmethylamino, methylpiperidinylmethylamino or imidazolemethylamino), heterocycloalkylcarbonylamino (pyrrolidinylcarbonylamino), heterocycloalkylalkylcarbonylamino optionally substituted with -OH (such as, for example, 2-piperidin-4-ylacetamide or 3- morpholinylpropanamide), heterocycloalkylcarbonylamino optionally substituted with 1-2 groups selected from halo and methyl (such as, for example, -N(H)C(O)piperidinyl optionally substituted with 1 or 2 groups selected from halo and methyl, -N(H)C(O)pyrrolidinyl, or -N(H)C(O)tetrahydrofuranyl,), heteroarylcarbonylamino optionally substituted with 1 or 2 groups selected from amino, alkyl, halo, -C(O)OH, pyrazolyl, -OCH3 and -C(O)OCH3 (such as, for example, -N(H)C(O)pyridinyl optionally substituted with 1 or 2 groups selected from -NH2, methyl, halo, -C(O)OH, pyrazolyl, -OCH3 and
-C(O)OCH3, -N(H)C(O)imidazolyl optionally substituted with methyl, -N(H)C(O)quinoxalinyl, -N(H)C(O)pyrimidinyl optionally substituted with alkyl, -N(H)C(O)furanyl, -N(H)C(O)pyrazinyl, -N(H)C(O)isoxazolyl optionally substituted with methyl, -N(H)C(O)oxazolyl, or -N(H)C(O)indazolyl optionally substituted with amino), -N(H)C(O)phenyl optionally substituted with 1 or 2 groups selected from halo, methyl, methoxy and -NHC(O)CH3, -N(H)C(O)alkyl, -N(H)C(O)(C1- C3)alkylpyridinyl, -N(H)C(O)(Ci-C3)alkylpiperidinyl, -N(H)C(O)-lH-pyrrolo[2,3-b]pyridinyl,
-N(H)C(O)cyclohexyl, N(H)C(O)cyclopentyl, -N(H)C(O)(C i-C3)alkylmorpholinyl, -N(H)C(O)(Ci-C3)alkylpyridinyl, -N(H)C(O)(Ci-C3)alkylimidazolyl, aminoalkyl (such as, for example, aminomethyl,), and -N(H)C(O)N(H)pyrimidinyl. [0059] In another embodiment (5), R2 is methylpiperazinylphenylalkoxyalkyl (methylpiperazinylphenylmethoxymethyl), methylpiperazinylcarbonyl, 2- chlorophenyl-4-methylpiperazinylmethyl, (4-methylpiperazin- 1 -yl)(phenyl)methyl, 1 - (4-methylpiperazin- 1 -yl)-2-phenylethyl, or 2-chlorophenyl(4-methylpiperazin- 1 - yl)methyl, 4-oxo-3-phenyl-l,3,8-triazaspiro[4.5]dec-l-yl)methyl. In another embodiment (6), R2 is -(Ci-C3)alkylC(O)OH. In another embodiment (7), R2 is hydroxyalkyl.
In another embodiment (8), R2 is -(Ci-C3)alkyl-N(Rz)-aryl (wherein aryl can be, for example, phenyl or 2,3-dihydro-lH-indenyl) optionally substituted with chloro, fluoro, piperazinyl, methylpiperazinyl or dialkylamino(dimethylamino), and Rz is H or methyl.
[0060] In another embodiment (9), R2 is -(5-10)membered heteroaryl (such as, for example, thienyl, pyridinyl, indazolyl, imidazolyl, thiazolyl, isoxazolyl, pyrazolyl, pyrimidinyl, benzimidazoly, pyrazolo[l,5-a]pyrimidinyl, tetrahydroisoquinolinyl, or 5,6,7,8-tetrahydroimidazo[l,2-a]pyrazinyl), optionally substituted with 1 or 2 groups selected from halo, -(Ci-Ce)alkyl, -(Ci-Ce)alkoxy, piperidinylalkylamino, piperdinylalkylamino, alkylamino, aminoalkyl, dialkylaminoalkylamino, piperidinyloxy, piperidinyl, and amino. Non- limiting examples of this embodiment include 5-chloro-2-thienyl, thienyl, 3 -methyl- lH-indazo IyI, 3 -methyl- lH-indazo IyI, 3- [(2-methylpropyl)oxy]pyridin-4-yl, 3-amino-lH-indazolyl, 3 -amino-5 -chloro- IH- indazolyl, lH-benzimidazolyl, lH-imidazolyl, 1,3-thiazolyl, 2-amino-5- chloropyrimidin-4-yl, 3-[(piperidin-4-ylmethyl)amino]-lH-indazolyl, and (2S)-2,3- dihydro- 1 H-indol-2-yl. [0061] In another embodiment(lθ), R2 is -(C1-C3)alkyl-O-phenyl. [0062] In another embodiment (11), R2 is -(Ci-C3)alkyl-O-(Ci-C3)alkyl-(5-6 membered)heterocycloalkyl, wherein the heterocycloalkyl can be, for example, pyrrolidinyl.
[0063] In another embodiment (12), R2 is -(Ci-C3)alkyl-N(H)-heteroaryl wherein the heteroaryl can be, for example, pyridinyl. [0064] In another embodiment (13), R2 is -(Ci-C3)alkyl-(5-10)membered heteroaryl (wherein the heteroaryl can be, for example, imidazolyl or pyrazolyl) optionally substituted with -(Ci-C3)alkyl, halo and phenyl. [0065] In another embodiment (14), R2 is oxopyrrolidinyl optionally substituted with OH and/or piperidinyl.
[0066] In another embodiment (15), R2 is -(Ci-C4)alkyl-(3-9 membered)heterocycloalkyl optionally substituted at any position of the (3-9 membered)heterocycloalkyl with Xd, wherein Xd is selected from alkyl( such as, for example, methyl), 1-3 halo, -COOH, phenyl optionally substituted with 1 or 2 groups selected from halo, methyl and methylphenyl, phenylmethyl, spiro-piperidine, trifluoromethylphenylmethyl, -(Ci-C3)alkoxy, pyridinyl, dimethylaminoalkyl (such as, for example, dimethylamino-(Ci-C3)alkyl), dimethylamino, hydroxylalkyl, dimethylaminoalkylaminocarbonyl (such as, for example, dimethylamino(Ci-
C3)alkylaminocarbonyl), alkylamino, aminoalkyl (such as, for example, aminomethyl or aminoethyl), dimethylamino carbonylalkyl (such as, for example, dimethylaminocarbonylmethyl), diethylaminoalkylcarbonyl (such as, for example, diethylaminomethylcarbonyl), -(Ci-C3)alkyl-(5-6 membered)heterocycloalkyl, (5-6 membered)heterocycloalkyl (such as, for example, piperidinyl or morpholinyl) optionally substituted with -(Ci-C3)alkyl, -NH2, -OH, heterocycloalkylalkylamino, alkoxyalkyl (such as for example, methoxy ethyl), -C(O)CH3, -C(O)NH(Ci- C3)alkylphenyl optionally substituted with 1-3 halo at any phenyl position, one -OH and one -C(O)NH(C i-C3)alkylphenyl optionally substituted with 1-3 halo at any phenyl position,-C(O)-heterocycloalkyl ( such as, for example, morpholinyl or piperidinyl) optionally substituted with -OH or halo, alkoxycarbonyl, aminocarbonyl, one hydroxyl and one methyl, one -OH and one -C(O)OH, one -OH and one - NHC(O)piperidinyl, and one -OH and one alkyl. In this embodiment, the (3-9 membered)heterocycloalkyl can be, for example, piperazinyl, piperidinyl, pyrrolidinyl, azetidinyl, morpholinyl, 1 ,4-diazepanyl, 2,5-diazabicyclo[2.2.1]heptyl, azabicyclo[2.2.1]heptane such as anti-7-hydroxy-2-azabicyclo[2.2.1]heptane, 7- hydroxy-2-azabicyclo[2.2.1]heptanyl and (7S)-7-hydroxy-2- azabicyclo[2.2.1]heptanyl, 8-azabicylo[3.2.1]oct-8-yl such as 3-hydroxy-8- azabicylo[3.2.1]oct-8-yl, (lS,4S)-2,5-diazabicyclo[2.2.1]heptyl such as (lS,4S)-5- methyl-2,5-diazabicyclo[2.2.1]heptyl and (lS,4S)-5-ethyl-2,5- diazabicyclo[2.2.1]heptyl, 2,5-dihydro-lH-pyrrolyl, (lR,4R)-2,5- diazabicyclo[2.2.1]heptan-2-yl such as (lR,4R)-5-methyl-2,5- diazabicyclo[2.2.1]heptan-2-yl, (lR,5S)-8-azabicyclo[3.2.1]octyl such as (lR,5S)-3- amino-8-azabicyclo[3.2.1]octyl, (8aS)-hexahydropyrrolo[l,2-a]pyrazin-2(lH)-yl, dimethylpiperazinyl such as (2R,6S)-2,6-dimethylpiperazinyl or (3aR,6aS)- hexahydropyrrolo[3,4-c]pyrrolyl. In another embodiment, the -(Ci-C4)alkyl-(3-9 membered)heterocycloalkyl described above is -CH2-(5-7 membered)heterocycloalkyl, which can be optionally substituted with any of the optional substituents described above for -(Ci-C4)alkyl-(3-9 membered)heterocycloalkyl. In another embodiment, R2 is -(CH2)-(5-6 membered)heterocycloalkyl optionally substituted with any of the optional substituents described above for -(Ci-C4)alkyl-(3-9 membered)heterocycloalkyl. In another embodiment, Xd is bonded to the -(3-9 membered)heterocycloalkyl portion of the -(Ci-C4)alkyl-(3-9 membered)heterocycloalkyl in the (S) stereochemical configuration. In another embodiment, Xd is bonded to the -(3-9 membered)heterocycloalkyl portion of the -(Ci-C4)alkyl-(3-9 membered)heterocycloalkyl in the (R) stereochemical configuration. [0067] In another embodiment (16), R2 is -(C1-C6)alkyl-NRz-(C1-C4)alkyl wherein the -(Ci-C4)alkyl portion is optionally substituted with Xe, and Xe is selected from dialkylamino (such as, for example, dimethylamino), amino, 1-3 -OH, alkoxy, A- methylpiperazinylphenyl, dimethylaminophenyl, phenyl optionally substituted with 1- 3 groups selected from halo and methoxy, heteroaryl (such as, for example, furanyl, pyridinyl or imidizolyl), -(Ci-C3)alkylC(O)NH2, -C(O)NH2, -C(O)OH, -(C1-
C3)alkylC(O)OH, heterocycloalkyl (such as, for example, morpholinyl, pyrrolidinyl piperidinyl or piperazinyl) optionally substituted with 1-2 alkyl (non-limiting examples include l,l-dimethyl-2 -pyrrolidinyl, l,l-dimethyl-2-piperidinyl, and 4- methylpiperazinyl), wherein Rz is H or alkyl optionally substituted with 1-3 -OH. In another embodiment, the -(C1-C6)alkyl-NRz-(C1-C4)alkyl above is -(C1-C3)alkyl- NRz-(C 1-C4)alkyl which can be optionally substituted with any of the optional substituents described above for -(C1-C6)alkyl-NRz-(C1-C4)alkyl. [0068] In another embodiment (17), R2 is -(Ci-C3)alkyl-NH-(C3-C6)cycloalkyl such as, for example, -(CH2)-NH-(C3-C6)cyclohexyl. [0069] In another embodiment (18), R2 is -(Ci-C3)alkyl-NH2 , wherein the -(C1- C3)alkyl- potion is optionally substituted with Xf, and Xf is selected from cycloalkyl (such as, for example, cyclohexyl), spirocycloalkyl, phenyl, phenylalkyl optionally substituted with phenylmethyloxy or alkoxy and thienylalkyl. Non-limiting examples of this embodiment include any one or more of the following groups: -(CH2)S-NH2, - CH2-NH2, -(CH2)2-NH2, -CH2-CH(CH3)-NH2, or -C(CH3)2-NH2. [0070] In another embodiment (19), R2 is (3-9 membered)heterocycloalkyl ( such as, for example, piperazinyl, piperidinyl, pyrrolidinyl, isoxazolyl, azetidinyl, morpholinyl, tetrahydrofuranyl, thiazolidinyl or octahydro-lH-indolyl) optionally substituted with Xg, and Xg is selected from alkyl (such as, for example (C1- C3)alkyl), alkylcarbonyl (such as, for example, -C(O)CH3), heterocycloalkylcarbonyl, dialkylaminoalkylcarbonyl (such as, for example, 4- (dimethylamino)butanoyl, 4-(dimethylamino)propanoyl or 2- (dimethylamino)ethanoyl) 1 -methylpiperidinyl, dialkylaminoalkyl (such as, for example, -(dimethylamino)ethyl or 3-(dimethylamino)propyl), heteroarylcarbonyl (such as, for example, l-(lH-benzimidazol-5-ylcarbonyl)piperidinyl or pyridinylcarbonyl), alkoxyalkylcarbonyl (such as, for example, 3- (methyloxy)propanoyl), phenylcarbonyl, phenylalkylcarbonyl, oxo, phenylalkyl, (5-6 membered)heteroarylalkyl (such as, for example, pyridinylmethyl), piperidinyloxy, - OH, oxo, 1-2 halo and 1-2 methyl. In another embodiment where R2 is (3-9 membered)heterocycloalkyl, R2 is bonded to the parent moiety in the (S) stereochemical configuration. In another embodiment where R2 is (3-9 membered)heterocycloalkyl, R2 is bonded to the parent moiety in the (R) stereochemical configuration. In another embodiment, R2 is (5 membered)heterocycloalkyl optionally substituted with methyl. In another embodiment, R2 is (5 membered)heterocycloalkyl optionally substituted with halo. In another embodiment, R2 is (5 membered)heterocycloalkyl optionally substituted with-OH. In another embodiment, R2 is (5 membered)heterocycloalkyl optionally substituted with phenylmethyl. In another embodiment, R2 is (5 membered)heterocycloalkyl optionally substituted with -C(O)CH3. In another embodiment, R2 is (5 membered)heterocycloalkyl optionally substituted with dialkylaminoalkylcarbonyl. In another embodiment, R2 is (5 membered)heterocycloalkyl optionally substituted with dialkylaminoalkyl heteroarylcarbonyl. In another embodiment, R2 is (6 membered)heterocycloalkyl optionally substituted with methyl. In another embodiment, R2 is (6 membered)heterocycloalkyl optionally substituted with halo. In another embodiment, R2 is (6 membered)heterocycloalkyl optionally substituted with-OH. In another embodiment, R2 is (6 membered)heterocycloalkyl optionally substituted with phenylmethyl. In another embodiment, R2 is (6 membered)heterocycloalkyl optionally substituted with -C(O)CH3. In another embodiment, R2 is (6 membered)heterocycloalkyl optionally substituted with dialkylaminoalkylcarbonyl. In another embodiment, R2 is (6 membered)heterocycloalkyl optionally substituted with dialkylaminoalkyl heteroarylcarbonyl.
[0071] In another embodiment (20), R2 is -(C3-C6)-cycloalkyl (cyclohexyl) optionally substituted with amino, -NHC(O)-O-(CH3)3 or aminoalkyl (such as, for example, aminomethyl). [0072] In another embodiment (21), R2 is dialkylaminoalkylamino, such as, for example 3-(dimethylamino)propylamino. In another embodiment (22), R2 is
Figure imgf000026_0001
, wherein Ri 5 is selected from H or -(Ci -C6)alkyl, Ri6 is selected from H, phenyl and -(Ci-C6)alkyl, and Rn is selected from H, -(Ci-C3)alkylC(O)NH2, -(Ci-C3)alkylC(O)OH and heterocycloalkylalkyl (such as, for example, 1,1-dimethyl- 2-pyrrolidin- 1 -ylethyl or 1 , 1 -dimethy 1-2-piperidin- 1 -ylethyl) .
[0073] All compounds of formula I for each of Aspect A (and Aspect B as applicable) disclosed above include any of the disclosed alternative aspects or embodiments for each of Ri, R2, R3a, R3b, R3C, or R3d, in combination with any other of the disclosed alternative aspects or embodiments of R1, R2, R3a, R3b, R3c, or R3d, as well as any pharmaceutically acceptable salt and stereoisomer of any such combination.
[0074] Within this disclosure, when a chemical moiety is said to have one or more optional substituents on any ring portion, this is meant to mean the same as when a chemical moiety can have one or more optional substituents on any ring position, which is meant to be the same as when as when a chemical moiety can have one or more optional substituents on a ring, wherein each of the one or more optional substituents replaces any hydrogen atom on any position of the ring, and if there is more than one substituent, then the remaining substituent(s) can replace any other of the remaining hydrogens on this ring. [0075] In other embodiments, any of the alkyl groups referred to in any of the above embodiments, including alkyl portions attached to other groups, can be a -(C1- Ce)alkyl group.
[0076] In other embodiments, any of the alkyl groups referred to in any of the above embodiments, including alkyl portions attached to other groups, can be a -(C1- C3)alkyl group.
[0077] In other embodiments, any of the alkoxy groups referred to in any of the above embodiments, including alkoxy portions attached to other groups, can be a - (Ci-Ce)alkoxy group. [0078] In other embodiments, any of the alkoxy groups referred to in any of the above embodiments, including alkoxy portions attached to other groups, can be a - (Ci-C3)alkoxy group.
[0079] In other embodiments, any of the heterocycloalkyl groups referred to in any of the above embodiments, including heterocycloalkyl portions attached to other groups, can be a (4-6 membered) heterocycloalkyl group.
[0080] In other embodiments, any of the cycloalkyl groups referred to in any of the above embodiments, can be a -(C3-Ce)cycloalkyl group.
[0081] All of the compounds disclosed herein include either their free base form or their pharmaceutically acceptable salts whether it is stated in the specification that these compounds can exist as their pharmaceutically acceptable salt or not. So, for instance, for any given embodiment of the compound of Formula I (including embodiments relating to the compounds themselves or method of use thereof), this embodiment includes either its free base form or any of its pharmaceutically acceptable salts, whether this is stated within this embodiment or not. [0082] Table 1 illustrates some examples of the compounds of this disclosure that are encompassed within formula I, and their pharmaceutically acceptable salts. The examples in Table 1 are merely illustrative, and do not limit the scope of the invention in any way. TABLE 1 , ,
Figure imgf000028_0001
Figure imgf000029_0001
,
Figure imgf000030_0001
,
[ [
Figure imgf000031_0001
Figure imgf000032_0001
,
Figure imgf000033_0001
Figure imgf000034_0001
Figure imgf000035_0001
Figure imgf000036_0001
[
[
Figure imgf000037_0001
[
Figure imgf000038_0001
Figure imgf000039_0001
,
,
Figure imgf000040_0001
,
Figure imgf000041_0001
[
Figure imgf000042_0001
,
Figure imgf000043_0001
,2
[
Figure imgf000044_0001
[
[
Figure imgf000045_0001
,2
[3
Figure imgf000046_0001
[
Figure imgf000047_0001
[
[
Figure imgf000048_0001
, , [
Figure imgf000049_0001
,
Figure imgf000050_0001
[
Figure imgf000051_0001
[ [ ,
Figure imgf000052_0001
,
Figure imgf000053_0001
,
Figure imgf000054_0001
Figure imgf000055_0001
Figure imgf000056_0001
Cpd
Structure NAME / ACTIVITY
No.
2-azetidin-3-yl-8- bromo[ 1 ]benzofuro[3,2-
198 d]pyrimidin-4(3H)-one Activity = A, B and C
8-bromo-2-
[(cyclopentylamino)methy
199 l][l]benzofuro[3,2- d]pyrimidin-4(3H)-one Activity = A, B and C
8-bromo-2-({[2- (dimethylamino)ethyl] ami
200 no}methyl)[ 1 ]benzofuro[3 ,2-d]pyrimidin-4(3H)-one Activity = B
8-bromo-2-[(4- methylpiperidin- 1 - yl)methyl] [ 1 ]benzofuro [3 ,
201 2-d]pyrimidin-4(3H)-one
Figure imgf000057_0001
Activity = A, B and C
2-(l,4'-bipiperidin-r- ylmethyl)-8- bromo[ 1 ]benzofuro[3,2-
202 d]pyrimidin-4(3H)-one
Figure imgf000057_0002
Activity = B
2-(l -acetylpiperidin-4-yl)- 8-bromo[ 1 ]benzofuro[3,2- d]pyrimidin-4(3H)-one
203 Activity = B and C
Figure imgf000057_0003
2-{[(3S)-3- hydroxypyrrolidin- 1 - yljmethyl} [l]benzofuro[3,
204 2-d]pyrimidin-4(3H)-one Activity = B and C
Figure imgf000057_0004
Figure imgf000058_0001
[ 1 ]
[
Figure imgf000059_0001
Figure imgf000060_0001
,
Figure imgf000061_0001
,2 , 2-
Figure imgf000062_0001
Cpd
Structure NAME / ACTIVITY
No.
8-bromo-2-({[(2- chloropheny l)methy 1] amin o}methyl)[ 1 ]benzofuro[3,
235 2-d]pyrimidin-4(3H)-one Activity = A, B and C
Figure imgf000063_0001
8-bromo-2-[ 1 -(pyridin-4- ylcarbonyl)piperidin-4- yl][l]benzofuro[3,2-
236 d]pyrimidin-4(3H)-one Activity = B and C
8-bromo-2-[l- (phenylacetyl)piperidin-4- yl][l]benzofuro[3,2-
237 d]pyrimidin-4(3H)-one Activity = B and C
Figure imgf000063_0002
8-bromo-2-[(4- phenylpiperidin- 1 - yl)methyl] [ 1 Jbenzofuro [3 ,
238 2-d]pyrimidin-4(3H)-one
Figure imgf000063_0003
Activity = B and C
8-bromo-2-
{ [(phenylmethyl)amino]m ethyl} [1 Jbenzofuro [3, 2-
239 d]pyrimidin-4(3H)-one
Activity = B and C
Figure imgf000063_0004
8-bromo-2-[(4-pyridin-3- ylpiperazin-1-
"NH yl)methyl] [ 1 Jbenzofuro [3 ,
240 2-d]pyrimidin-4(3H)-one Activity = B and C ,
,
Figure imgf000064_0001
Cpd Structure NAME / ACTIVITY
No.
8-bromo-2-{[4-(2- hydroxyethyl)piperazin- 1 - yl]methyl} [l]benzofuro[3,
247 2-d]pyrimidin-4(3H)-one Activity = B and C
Figure imgf000065_0001
N-[4-(8-bromo-4-oxo-3,4- dihydro [ 1 ]benzofuro [3 ,2- d]pyrimidin-2-yl)-3 -
248 chlorophenyl]-3- morpholin-4- ylpropanamide Activity = A, B and C
N-[4-(8-bromo-4-oxo-3,4- dihydro [ 1 ]benzofuro [3 ,2- d]pyrimidin-2-yl)-3 -
249 chlorophenyljbenzamide Activity = B and C
N-[4-(8-bromo-4-oxo-3,4- dihydro [ 1 ]benzofuro [3 ,2- d]pyrimidin-2-yl)-3 -
250 chloropheny 1] cy clohexane carboxamide Activity = B and C
N-[4-(8-bromo-4-oxo-3,4- dihydro [ 1 ]benzofuro [3 ,2- d]pyrimidin-2-yl)-3 -
251 chlorophenyljcyclopentan ecarboxamide
Figure imgf000065_0002
Activity = B and C
8-bromo-2-
[(cyclobutylamino)methyl ][l]benzofuro[3,2-
252 d]pyrimidin-4(3H)-one Activity = A, B and C
Figure imgf000065_0003
,
Figure imgf000066_0001
[ 1 ]
,
Figure imgf000067_0001
Figure imgf000068_0001
Figure imgf000069_0001
-
Figure imgf000070_0001
,
Figure imgf000071_0001
Cpd
Structure NAME / ACTIVITY
No.
8-bromo-2-(l- methylpyrrolidin-3 - yl)[l]benzofuro[3,2-
289 d]pyrimidin-4(3H)-one Activity = A, B and C
Figure imgf000072_0001
8-bromo-2-[ 1 -(pyridin-4- ylmethyl)pyrrolidin-3 - yl][l]benzofuro[3,2-
290 d]pyrimidin-4(3H)-one Activity = A, B and C
N-[4-(8-bromo-4-oxo-3,4- dihydro [ 1 ]benzofuro [3 ,2- d]pyrimidin-2-yl)-3 -
291 chlorophenyl] -6- methylpyridine-3 -
Figure imgf000072_0002
carboxamide Activity = A, B and C
N-[4-(8-bromo-4-oxo-3,4- dihydro [ 1 ]benzofuro [3 ,2- d]pyrimidin-2-yl)-3 -
292 chlorophenyl]pyridine-3 - carboxamide
Figure imgf000072_0003
Activity = B and C
N-[4-(8-bromo-4-oxo-3,4- dihydro [ 1 ]benzofuro [3 ,2- d]pyrimidin-2-yl)-3-
293 chlorophenyl]pyridine-2- carboxamide
Figure imgf000072_0004
Activity = A, B and C
N-[4-(8-bromo-4-oxo-3,4- dihydro [ 1 ]benzofuro [3 ,2- d]pyrimidin-2-yl)-3-
294 chloropheny 1] -2,6- dichloropyridine-4-
Figure imgf000072_0005
carboxamide
Activity = A, B and C Cpd
Structure NAME / ACTIVITY
No.
8-bromo-2-{[(lS,4S)-5- ethyl-2,5- diazabicyclo[2.2.1]hept-2-
295 yljmethyl} [l]benzofuro[3, 2-d]pyrimidin-4(3H)-one Activity = B and C
8-bromo-2-{[(3R,5S)-3,5- dimethylpiperazin- 1 - yljmethyl} [l]benzofuro[3,
296 2-d]pyrimidin-4(3H)-one Activity = B
Figure imgf000073_0001
l,l-dimethylethyl 2-(8- bromo-4-oxo-3,4- dihydro [ 1 ]benzofuro [3 ,2-
297 d]pyrimidin-2- yl)morpholine-4- carboxylate
Activity = B and C
8-bromo-2-morpholin-2- yl[l]benzofuro[3,2- d]pyrimidin-4(3H)-one
298 Activity = A, B and C
8-bromo-2-[3-(piperidin- 4-yloxy)isoxazol-5 - yl][l]benzofuro[3,2-
299 d]pyrimidin-4(3H)-one Activity = B and C
8-bromo-2-(l,2,3,4- tetrahydroisoquinolin-6- yl)[l]benzofuro[3,2-
300 d]pyrimidin-4(3H)-one Activity = B and C
Figure imgf000073_0002
- ,2 ,2
Figure imgf000074_0001
Figure imgf000075_0001
,2
, ,
Figure imgf000076_0001
Figure imgf000077_0001
Cpd
Structure NAME / ACTIVITY
No. l-[(8-bromo-4-oxo-3,4- dihydro [ 1 ]benzofuro [3 ,2- d]pyrimidin-2-yl)methyl]-
325 N-(pyridin-4- ylmethyl)pyrrolidine-3 -
Figure imgf000078_0001
carboxamide
Activity = B and C
N-[3-(8-bromo-4-oxo-3,4- dihydro [ 1 ]benzofuro [3 ,2- d]pyrimidin-2-yl)-4-
326 chlorophenyl] -2- chloropyridine-4-
Figure imgf000078_0002
carboxamide
Activity = B and C
N-[3-(8-bromo-4-oxo-3,4- dihydro [ 1 ]benzofuro [3 ,2- d]pyrimidin-2-yl)-4-
327 chlorophenyl]pyridine-3 -
Figure imgf000078_0003
carboxamide Activity = B
N-[4-(8-bromo-4-oxo-3,4- dihydro [ 1 ]benzofuro [3 ,2- d]pyrimidin-2-yl)-3-
328 chlorophenyljpyrimidine- 5 -carboxamide
Figure imgf000078_0004
Activity = A, B and C
N-[3-(8-bromo-4-oxo-3,4- dihydro [ 1 ]benzofuro [3 ,2- d]pyrimidin-2-yl)-4-
329 chlorophenyljpyrimidine- 5 -carboxamide
Figure imgf000078_0005
Activity = B and C
8-bromo-2-{[(lS,4S)-5- methy 1-2,5 - diazabicyclo[2.2.2]oct-2-
330 yl]methyl} [l]benzofuro[3, 2-d]pyrimidin-4(3H)-one
Figure imgf000078_0006
Activity = A, B and C
Figure imgf000079_0001
Figure imgf000080_0001
,
Figure imgf000081_0001
,
Figure imgf000082_0001
Figure imgf000083_0001
Cpd
Structure NAME / ACTIVITY
No.
N-[4-(8-bromo-4-oxo-3,4- dihydro [ 1 ]benzofuro [3 ,2- d]pyrimidin-2-
360 yl)phenyl]pyridine-4- carboxamide
Figure imgf000084_0001
Activity = B and C
N-[3-(8-bromo-4-oxo-3,4- dihydro [ 1 ]benzofuro [3 ,2- d]pyrimidin-2-yl)-4-
361 chlorophenyljtetrahydrofu
Figure imgf000084_0002
ran-3 -carboxamide Activity = B and C
N-[4-(8-bromo-4-oxo-3,4- dihydro [ 1 ]benzofuro [3 ,2- d]pyrimidin-2-yl)-3 -
362 chlorophenyl]-5- methylisoxazole-3-
Figure imgf000084_0003
carboxamide
Activity = A, B and C
N-[4-(8-bromo-4-oxo-3,4- dihydro [ 1 ]benzofuro [3 ,2- d]pyrimidin-2-yl)-3 -
363 chlorophenyl]isoxazole-3- carboxamide
Figure imgf000084_0004
Activity = A, B and C
N-{(3R)-l-[(8-bromo-4- oxo-3,4- dihydro [ 1 ]benzofuro [3 ,2-
364 d]pyrimidin-2- yl)methyl]pyrrolidin-3 -
Figure imgf000084_0005
yl}acetamide
Activity = A, B and C
N-{(3S)-l-[(8-bromo-4- oxo-3,4- dihydro [ 1 ]benzofuro [3 ,2-
365 d]pyrimidin-2- yl)methyl]pyrrolidin-3 -
Figure imgf000084_0006
yl}acetamide
Activity = B and C Cpd
Structure NAME / ACTIVITY
No.
8-bromo-2-{[(2S)-2-
(hydroxymethyl)pyrrolidin
-1- yljmethyl} [l]benzofuro[3,
2-d]pyrimidin-4(3H)-one
366 Activity = A, B and C
8-fluoro-2-[(4- methylpiperazin- 1 - yl)methyl] [ 1 ]benzofuro [3 ,
367 2-d]pyrimidin-4(3H)-one Activity = B
Figure imgf000085_0001
l-[(8-bromo-4-oxo-3,4- dihydro [ 1 ]benzofuro [3 ,2- d]pyrimidin-2-yl)methyl]-
368 3 -hydroxypyrrolidine-3 - carboxylic acid
Figure imgf000085_0002
Activity = A, B and C
8-bromo-2-{[(3S)-3- (methyloxy)pyrrolidin- 1 - yl]methyl} [l]benzofuro[3,
369 2-d]pyrimidin-4(3H)-one Activity = A, B and C
Figure imgf000085_0003
l-[(8-bromo-4-oxo-3,4- dihydro [ 1 ]benzofuro [3 ,2- d]pyrimidin-2-
370 yl)methyl]azetidine-3- carboxylic acid
Figure imgf000085_0004
Activity = A, B and C
N-[3-(8-bromo-4-oxo-3,4- dihydro [ 1 ]benzofuro [3 ,2- d]pyrimidin-2-yl)-4-
371 chlorophenyljbenzamide Activity = B and C
Figure imgf000085_0005
Figure imgf000086_0001
Figure imgf000087_0001
, phe 1-
Figure imgf000088_0001
1-
1-
Figure imgf000089_0001
,
,
Figure imgf000090_0001
,
Figure imgf000091_0001
,
Figure imgf000092_0001
Figure imgf000093_0001
,
[
Figure imgf000094_0001
[
Figure imgf000095_0001
,
Figure imgf000096_0001
[
[
Figure imgf000097_0001
- , [
-
Figure imgf000098_0001
Figure imgf000099_0001
Figure imgf000100_0001
Figure imgf000101_0001
-
-
Figure imgf000102_0001
Figure imgf000103_0001
Figure imgf000104_0001
Cpd
Structure NAME / ACTIVITY
No.
2-[(2S)-pyrrolidin-2-
506 yl][l]benzofuro[3,2- d]pyrimidin-4(3H)-one Activity = A, B and C
8-chloro-2-[(2S)- pyrrolidin-2-
507 yl][l]benzofuro[3,2- d]pyrimidin-4(3H)-one Activity = A, B and C
8-bromo-2-{l-[(3S)-3- hydroxypyrrolidin- 1 -
508 yl]ethyl} [l]benzofuro[3,2- d]pyrimidin-4(3H)-one Activity = A, B and C
2-[(2S)-azetidin-2-yl]-8-
509 bromo[ 1 ]benzofuro[3,2- d]pyrimidin-4(3H)-one Activity = A, B and C
8-bromo-2-[(2S)-2,3-
510 dihydro-lH-indol-2- yl][l]benzofuro[3,2- d]pyrimidin-4(3H)-one Activity = A, B and C
8-bromo-2-[(2S,4S)-4- hydroxypyrrolidin-2-
511 yl][l]benzofuro[3,2- d]pyrimidin-4(3H)-one Activity = A, B and C
8-bromo-2-[(2S)-4,4-
512 difluoropyrrolidin-2- yl][l]benzofuro[3,2- d]pyrimidin-4(3H)-one
Figure imgf000105_0001
Activity = A, B and C Cpd
Structure NAME / ACTIVITY
No.
8-bromo-2-[(2S,4S)-4-
513 fluoropyrrolidin-2- yl][l]benzofuro[3,2- d]pyrimidin-4(3H)-one Activity = A, B and C
8-bromo-2-[(2S,4R)-4-
514 fluoropyrrolidin-2- yl][l]benzofuro[3,2- d]pyrimidin-4(3H)-one Activity = A, B and C
8-bromo-2-[(2S)-5,5-
515 dimethylpyrrolidin-2- yl][l]benzofuro[3,2- d]pyrimidin-4(3H)-one Activity = A, B and C
8-bromo-2-
[(methylamino)methyl] [ 1 ]
516 benzofuro[3,2- d]pyrimidin-4(3H)-one Activity = A, B and C
8-bromo-2-
[(dimethylamino)methyl] [
517 l]benzofuro[3,2- d]pyrimidin-4(3H)-one Activity = A, B and C
8-bromo-2- { [methyl( 1 -
518 methylpropyl)amino]meth yl}[l]benzofuro[3,2- d]pyrimidin-4(3H)-one Activity = A, B and C
8-bromo-2-({[(lR)-l-
519 methy lpropy 1] amino } meth yl)[l]benzofuro[3,2- d]pyrimidin-4(3H)-one
Figure imgf000106_0001
Activity = A, B and C
Figure imgf000107_0001
[ [ [
Figure imgf000108_0001
Figure imgf000109_0001
}
Figure imgf000110_0001
[0083] Activity A in Table 1 is meant to mean the compound has a CDC7 IC50 value of less than 10,000 nanomolar (nm).
[0084] Activity B in Table 1 is meant to mean the compound has a PIM IC50 value of less than 10,000 nanomolar (nm). [0085] Activity C in Table 1 is meant to mean the compound has a CK2 IC50 value of less than 10,000 nanomolar (nm).
[0086] When the activity for any of the compounds in Table 1 lists more than one target for which the compound has activity against (as defined by Activity A, B and C above), then the compound in question is meant to be active against each of the targets that is listed next to the compound.
[0087] Another aspect of this dislcosure relates to a pharmaceutical composition comprising a compound according to Formula I, or a pharmaceutically acceptable salt thereof, and a pharmaceutically acceptable carrier, excipient, or diluent. [0088] Another aspect of this dislcosure relates to a method of inhibiting PIM, CDC7 or CK2 in a cell, comprising contacting a cell in which inhibition of PIM, CDC7 or CK2 is desired with a compound according to Formula I, or a pharmaceutically acceptable salt thereof. [0085] Another aspect of this dislcosure relates to a method of inhibiting PIM, CDC7 or CK2 in a cell, comprising contacting a cell in which inhibition of PIM, CDC7 or CK2 is desired with a pharmaceutical composition comprising a compound according to Formula I, or a pharmaceutically acceptable salt thereof, and a pharmaceutically acceptable carrier, excipient, or diluent. [0086] Another aspect of this dislcosure relates to a method of treating a disease or condition, including those that involve PIM, CDC7 or CK2, comprising administering to a patient, in need of the treatment, a compound according to Formula I, or a pharmaceutically acceptable salt thereof. Non- limiting examples of the disease or condition that can be treated include cancer such as ovarian cancer, pancreatic cancer, prostate cancer, hepatocellular carcinoma, lymphomas, leukemias, cervical cancer, breast cancer, colorectal cancer, non-small cell lung cancer (NSCL) or glioblastomas. In another embodiment, the disease or condition that can be treated is selected from pancreatic cancer, prostate cancer, hepatocellular carcinoma, lymphomas, leukemias, colorectal cancer and non-small cell lung cancer. In another embodiment, the disease or condition that can be treated is selected from pancreatic cancer, prostate cancer, hepatocellular carcinoma, lymphomas and leukemias. In another embodiment, the disease or condition that can be treated is selected from colorectal cancer and non-small cell lung cancer. [0087] Another aspect of this disclosure relates to a compound in Table 1 that has a CDC7 IC50 value of less than 3000 nm, or a pharmaceutically acceptable salt of such a compound. The compounds in Table 1 that have a CDC7 IC50 value of less than 3000 nm are compounds 1-3, 5, 13, 24, 28, 32-35, 40, 43, 45, 48, 49, 52, 60, 64, 100, 103, 107, 108-109, 111-112, 119, 121, 127-128, 130, 138, 140-144, 147-148, 153- 155, 157-161, 164, 166-167, 169, 173-175, 178, 181, 182-186, 189, 191, 194-195, 197-199, 201, 205, 214, 216-217, 220-221, 223-224, 226, 231-232, 235, 248, 252, 259, 262, 264, 266, 269, 270, 273, 277, 283, 287, 289-290, 298, 302, 305, 307, 308, 311, 313, 315, 317, 319, 323, 324, 330-333, 344, 346, 348, 349, 353, 363-364, 366, 368-370, 373-375, 379-381, 383, 385-389, 395, 400-402, 404, 406-408, 412-413, 418, 420, 425-427, 429, 430-435, 436-439, 442, 448, 451-453, 458-460, 469-471, 476, 488-490, 492-509, 511-535, and 537-544.
[0088] Another aspect of this disclosure relates to a compound in Table 1 that has a CDC7 IC50 value of less than 100 nm, or a pharmaceutically acceptable salt of such a compound. The compounds in Table 1 that have a CDC7 IC50 value of less than 100 nm are compounds 1-2, 5, 28, 49, 103, 108, 119, 128, 147, 157-158, 164, 166- 167, 169, 173-175, 183-186, 191, 198-199, 201, 214, 216, 221, 223, 231-232, 242, 252, 259, 262, 269, 270, 273, 277, 287, 289, 298, 307, 308, 317, 331-333, 344, 346, 348, 364, 366, 368-369, 375, 379-380, 383, 400-402, 404, 407-408, 412-413, 421, 426, 429, 432, 437, 442, 452, 453, 458, 470-471, 476, 488-490, 492, 493, 495, 496, 498, 501-503, 505, 507-509, 511-534, and 540-544.
[0089] Another aspect of this dislcosure relates to a method of treating a disease or condition, including those that involve PIM, CDC7 or CK2, comprising administering to a patient, in need of the treatment, a pharmaceutical composition comprising a compound according to Formula I, or a pharmaceutically acceptable salt thereof, and a pharmaceutically acceptable carrier, excipient, or diluent. Non-limiting examples of the disease or condition that can be treated include cancer such as ovarian cancer, pancreatic cancer, prostate cancer, hepatocellular carcinoma, lymphomas, leukemias, cervical cancer, breast cancer, colorectal cancer, non-small cell lung cancer (NSCL) or glioblastomas. In another embodiment, the disease or condition that can be treated is selected from pancreatic cancer, prostate cancer, hepatocellular carcinoma, lymphomas, leukemias, colorectal cancer and non-small cell lung cancer. In another embodiment, the disease or condition that can be treated is selected from pancreatic cancer, prostate cancer, hepatocellular carcinoma, lymphomas and leukemias. In another embodiment, the disease or condition that can be treated is selected from colorectal cancer and non-small cell lung cancer.
[0090] Another aspect of this dislcosure relates to a method of treating a disease or condition, including those that involve PIM, CDC7 or CK2 comprising administering to a patient, in need of the treatment, a compound according to Formula I, or a pharmaceutically acceptable salt thereof, in combination with radiation treatment and/or one or more therapeutic angents selected from Camptothecin, Topotecan, 9-Nitrocamptothecin, 9-Aminocamptothecin, Karenitecin, Irinotecan, Etoposide, Etoposide Phosphate, Teniposide, Amsacrine, Razoxane, Dexrazoxane, Mechlorethamine, Cyclophosphamide, Ifosfamide, Chlorambucil, Melphalan, Thiotepa, Trenimon, Triethylenemelamine, Rapamycin, Dianhydrogalactitol, Dibromodulcitol, Busulfan, dimethylsulfate, Chloroethylnitrosourea, BCNU, CCNU, Methyl-CCNU, Streptozotocin, Chlorozotocin, Prednimustine, Estramustine, Procarbazine, Dacarbazine, Hexamethylmelamine, Pentamethylmelamine, Temozolomide, Cisplatin, Carboplatin, Oxaliplatin, Bleomycin, Dactinomycin, Mithramycin, Mitomycin C, Daunorubicin, Doxorubicin, Epirubicin, Idarubicin, Methotrexate, Edatrexate, Trimethoprim, Nolatrexed, Raltitrexed, Hydroxyurea, 5- fluorouracil, Ftorafur, Capecitabine, Furtulon, Eniluracil, ara-C, 5-azacytidine, Gemcitabine, Mercaptopurine, Thioguanine, Pentostatin, antisense DNA, antisense RNA, an antisense DNA/RNA hybrid, a ribozyme, ultraviolet radiation, Vincristine, Vinblastine, Paclitaxel, Docetaxel, L-Asparaginase, a kinase inhibitor, Imatinib, Mitotane, Aminoglutethimide, Diethylstilbestrol, Ethinyl estradiol, Tamoxifen, Anastrozole, Testosterone propionate, Fluoxymesterone, ixabepilone, Flutamide, Leuprolide, Prednisone, Hydroxyprogesterone caproate, Medroxyprogesterone acetate, Megestrol acetate, Interferon-alfa, and Interleukin. In a more specific embodiment, the combination is with ixabepilone or etoposide.
[0091] Other aspects of this disclosure include the compounds from Table 1 that fall within any of the above embodiments of this disclosure, incuding Aspect B.
Abbreviations and Definitions
[0092] The following abbreviations and terms have the indicated meanings throughout:
Figure imgf000114_0001
Figure imgf000115_0001
Abbreviation Meanin
Figure imgf000116_0003
[0093] As used in the present specification, the following words and phrases are generally intended to have the meanings as set forth below, except to the extent that the context in which they are used indicates otherwise or they are expressly defined to mean something different. [0094] The symbol "-" means a single bond, "=" means a double bond, "=" means a triple bond, " " means a single or double bond. When a group is depicted removed from its parent formula, the 'ζ iΛΛΛ " symbol will be used at the end of the bond which was theoretically cleaved in order to separate the group from its parent structural formula.
[0095] When chemical structures are depicted or described, unless explicitly stated otherwise, all carbons are assumed to have hydrogen substitution to conform to a valence of four. For example, in the structure on the left-hand side of the schematic below there are nine hydrogens implied. The nine hydrogens are depicted in the right- hand structure. Sometimes a particular atom in a structure is described in textual formula as having a hydrogen or hydrogens as substitution (expressly defined hydrogen), for example,
-CH2CH2-. It is understood by one of ordinary skill in the art that the aforementioned descriptive techniques are common in the chemical arts to provide brevity and simplicity to description of otherwise complex structures.
Figure imgf000116_0001
[0096] If a group "R" is depicted as "floating" on a ring system, as for example in the formula:
Figure imgf000116_0002
then, unless otherwise defined, a substituent "R" can reside on any atom of the ring system, assuming replacement of a depicted, implied, or expressly defined hydrogen from one of the ring atoms, so long as a stable structure is formed. [0097] If a group "R" is depicted as floating on a fused ring system, as for example in the formulae:
Figure imgf000117_0001
then, unless otherwise defined, a substituent "R" can reside on any atom of the fused ring system, assuming replacement of a depicted hydrogen (for example the -NH- in the formula above), implied hydrogen (for example as in the formula above, where the hydrogens are not shown but understood to be present), or expressly defined hydrogen (for example where in the formula above, "X" equals =CH-) from one of the ring atoms, so long as a stable structure is formed. In the example depicted, the "R" group can reside on either the 5-membered or the 6-membered ring of the fused ring system. In the formula depicted above, when y is 2 for example, then the two "R's" can reside on any two atoms of the ring system, again assuming each replaces a depicted, implied, or expressly defined hydrogen on the ring.
[0098] When a group "R" is depicted as existing on a ring system containing saturated carbons, as for example in the formula:
Figure imgf000117_0002
where, in this example, "y" can be more than one, assuming each replaces a currently depicted, implied, or expressly defined hydrogen on the ring; then, unless otherwise defined, where the resulting structure is stable, two "R's" can reside on the same carbon. A simple example is when R is a methyl group; there can exist a geminal dimethyl on a carbon of the depicted ring (an "annular" carbon). In another example, two R's on the same carbon, including that carbon, can form a ring, thus creating a spirocyclic ring (a "spirocyclyl" group) structure with the depicted ring as for example in the formula:
Figure imgf000117_0003
[0099] "Administration" and variants thereof (e.g., "administering" a compound) in reference to a compound of this disclosure (i.e., a compound of Formula I as described herein) means introducing the compound or a prodrug of the compound into the system of the animal in need of treatment. When a compound of this disclosure or prodrug thereof is provided in combination with one or more other active agents (e.g., surgery, radiation, and chemotherapy, etc.), "administration" and its variants are each understood to include concurrent and sequential introduction of the compound or prodrug thereof and other agents.
[00100] "Alkyl" is intended to include molecules having 1-12 carbons in size (C1- Ci2)alkyl, which can be straight chained or branched. For example, "C6 alkyl" can refer to an n-hexyl, ώo-hexyl, cyclobutylethyl, and the like. Alkyl is intended to include lower alkyl groups of from 1-6 carbons in size, such as methyl, ethyl, propyl, isopropyl, butyl, s-butyl, t-butyl, isobutyl, pentyl, hexyl and the like. Higher alkyl refers to alkyl groups containing more that six carbon atoms. An alkyl residue having a specific number of carbons is named, all geometric isomers having that number of carbons are intended to be encompassed; thus, for example, either "butyl" or "C4 alkyl" is meant to include n-butyl, sec -butyl, isobutyl, t-butyl, and for example,
"propyl" or "C3 alkyl" each include n-propyl and isopropyl.
[00101] -(Ci-Ce)alkyl is a subset of alkyl groups that are from one to six carbon atoms in length, and can be straight chained or branched.
[00102] -(Ci-C3)alkyl is a subset of alkyl groups that are from one to three carbon atoms in length, and can be straight chained or branched.
[00103] "alkenyl" is intended to be an alkyl that contains at least one double bond between two carbons. Non-limiting examplels of alkenyl include vinyl, allyl, isoprenyl, and the like.
[00104] "alkynyl" is intended to be an alkyl that contains at least one triple bond between two carbons.
[00105] "Cycloalkyl" means a non-aromatic mono- or multicyclic ring system comprising about 3 to about 14 carbon atoms. Non-limiting examples of monocyclic cycloalkyls include cyclopropyl, cyclopentyl, cyclohexyl, cycloheptyl and the like. Non- limiting examples of multicyclic cycloalkyls include 1-decalin, norbornyl, adamantyl and the like. Cycloalkyls can be fused or bridge ring systems or spirocyclic systems. [00106] "-(C3-C6)cycloalkyl" is a subset of cycloalkyl and means a non-aromatic monocyclic ring system comprising from 3 to 6 carbon atoms.
[00107] "Alkoxy" or "alkoxyl" both refer to the group -O-alkyl, wherein the "alkyl" portion is as defined hereinabove. Examples include methoxy, ethoxy, propoxy, isopropoxy, and the like.
[00108] "Aryl" means a monovalent six- to fourteen-membered mono- or multicyclic ring, wherein the monocyclic ring is aromatic and at least one of the rings in the multicyclic ring is aromatic. A multicyclic ring that contains only one aryl ring is intended to be included within the definition of aryl. An aryl can also be six- to ten membered, or six membered. Representative non-limiting examples of aryl include phenyl, naphthyl, and the like.
[00109] "Arylalkyl" means a residue in which an aryl moiety, as defined above, is attached to a parent structure via one of an alkyl (i.e, alkylene, alkenylene, or alkynylene), wherein the "aryl" and "alkyl" portions are as defined herein. Examples include benzyl, phenethyl, phenylvinyl, phenylallyl and the like. The "alkyl" portion of the group can be one to ten carbons, and in another embodiment, one to six carbons; the latter can also be referred to as C \. β arylalkyl. When a group is referred to as or "-(Ci-Ce)alkylaryl," an aryl moiety is attached to a parent structure via an alkylene group. Examples include benzyl, phenethyl, and the like. [00110] In some examples, as appreciated by one of ordinary skill in the art, two adjacent groups on an aromatic system can be fused together to form a ring structure. The fused ring structure can contain heteroatoms and can be optionally substituted with one or more groups. It should additionally be noted that saturated carbons of such fused groups (i.e. saturated ring structures) can contain two substitution groups. [00111] "Fused-polycyclic" or "fused ring system" refers to a polycyclic ring system that contains bridged or fused rings; that is, where two rings have more than one shared atom in their ring structures. In this application, fused-polycyclics and fused ring systems includes non-aromatic and aromatic systems. Typically, but not necessarily, fused-polycyclics share a vicinal set of atoms, for example naphthalene or 1,2,3,4-tetrahydro-naphthalene. A spiro ring system is not a fused-polycyclic by this definition, but fused polycyclic ring systems of the compounds disclosed herein can themselves have spiro rings attached thereto via a single ring atom of the fused- polycyclic. [00112] "Halogen" or "halo" both refer to fluorine, chlorine, bromine or iodine. [00113] "Haloalkyl" (which includes alkyl, as defined herein, optionally substituted with up to 8 halo) and "haloaryl" refer generically to alkyl and aryl groups that are substituted with one or more halo, respectively. Non-limiting examples of "haloalkyl" include 3,3,3-trifluoro-l-methylpropyl, 2-methyl-l-(trifluoromethyl)propyl, -CH2F, - CHCl2 and -CF3.
[00114] "Heteroatom" refers to O, S, N, or P. In another example, the heteroatom is O or N. In another example, the heteroatom is O. In another example, the heteroatom is N. [00115] "Heterocyclyl" refers to a stable three- to fϊfteen-membered ring substituent that consists of carbon atoms and from one to five heteroatoms selected from the group consisting of nitrogen, phosphorus, oxygen and sulfur. For purposes of this disclosure, the heterocyclyl substituent can be a monocyclic, bicyclic or tricyclic ring system, which can include fused or bridged ring systems as well as spirocyclic systems. The terms "heterocycloalkyl" and "heteroaryl" are groups that are encompassed by the broader term "heterocyclyl." The nitrogen, phosphorus, carbon or sulfur atoms in the heterocyclyl group can be optionally oxidized to various oxidation states. In a specific example, the group -S(O)0-2-, refers to -S- (sulfide), - S(O)- (sulfoxide), and -SO2- (sulfone) respectively. For convenience, nitrogens, particularly but not exclusively, those defined as annular aromatic nitrogens, are meant to include their corresponding JV-oxide form, although not explicitly defined as such in a particular example. Thus, for a compound of this disclosure having, for example, a pyridyl ring; the corresponding pyridyl-iV-oxide is meant to be included as another compound of the disclosure. In addition, annular nitrogen atoms can be optionally quaternized; and the ring substituent can be partially or fully saturated or aromatic. Examples of heterocyclyl groups include, but are not limited to, azetidinyl, acridinyl, benzodioxolyl, benzodioxanyl, benzofuranyl, carbazoyl, cinnolinyl, dioxolanyl, indolizinyl, naphthyridinyl, perhydroazepinyl, phenazinyl, phenothiazinyl, phenoxazinyl, phthalazinyl, pteridinyl, purinyl, quinazolinyl, quinoxalinyl, quinolinyl, isoquinolinyl, tetrazoyl, tetrahydroisoquinolyl, piperidinyl, piperazinyl, 2- oxopiperazinyl, 2-oxopiperidinyl, 2-oxopyrrolidinyl, 2-oxoazepinyl, azepinyl, pyrrolyl, 4-piperidonyl, pyrrolidinyl, pyrazolyl, pyrazolidinyl, imidazolyl, imidazolinyl, imidazolidinyl, dihydropyridinyl, tetrahydropyridinyl, pyridinyl, pyrazinyl, pyrimidinyl, pyridazinyl, oxazolyl, oxazolinyl, oxazolidinyl, triazolyl, isoxazolyl, isoxazolidinyl, morpholinyl, thiazolyl, thiazolinyl, thiazolidinyl, isothiazolyl, quinuclidinyl, isothiazolidinyl, indolyl, isoindolyl, indolinyl, isoindolinyl, octahydroindolyl, octahydroisoindolyl, quinolyl, isoquinolyl, decahydroisoquinolyl, benzimidazolyl, thiadiazolyl, benzopyranyl, benzothiazolyl, benzoxazolyl, furyl, tetrahydrofuryl, tetrahydropyranyl, thienyl, benzothienyl, thiamorpholinyl, thiamorpholinyl sulfoxide, thiamorpholinyl sulfone, dioxaphospholanyl, oxadiazolyl, tetrahydrofuranyl, tetrahydroisoquinolinyl, and tetrahydroquinolinyl. [00116] "Heterocycloalkyl" refers to a stable 4-12 membered monocyclic or multicyclic ring, wherein at least one of the rings contain at least one heteroatom and wherein there are no aromatic rings. Heterocycloalkyl is meant to include multicyclic rings wherein one ring contains a heteroatom and another ring does not contain a heteroatom. [00117] "Heterocycloalkylalkyl" refers to a heterocycloalkyl, as defined herein, attached to the parent moiety through an "alkyl," as defined herein. One non-limiting example of heterocycloalkyl includes piperadinyl. Another non-limiting example of heterocycloalkyl includes piperazinyl. Another non-limiting example of heterocycloalkyl includes furanyl. Another non-limiting example of heterocycloalkyl includes pyrrolidinyl. Another non-limiting example of heterocycloalkyl includes morpholinyl.
[00118] "Amino" refers to -NH2.
[00119] "Alkylamino" refers to -NH(alkyl), wherein "alkyl" portion is as defined above, and wherein the parent moiety is attached to the nitrogen atom. [00120] "Dialkylamino" refers to -N(alkyl)2, wherein the "alkyl" portiobs are as defined above, and wherein the parent moiety is attached to the nitrogen atom. [00121] "Dialkylaminoalkyl" refers to -(alky l)N(alky I)2, wherein the "alkyl" portions are as defined above. One such non- limiting example of "dialkylaminoalkyl" includes -CH2C(CH3)2CH2N(CH3)2.
[00122] "Aminoalkyl" refers to -(alkyl)NH2, wherein "alkyl" is as defined above, and wherein the parent moiety is attached to the alkyl group. The amino group can be attached at any point along the alkyl group. Non-limiting examples of aminoalkyl include -CH2NH2, -CH2CH2NH2, -C(CH3)2NH2 , and -CH(NH2)CH3. [00123] "Heteroaryl" means a 5- to 12-membered, monocyclic aromatic heterocyclyl (where heterocyclyl is defined herein) or bicyclic heterocyclyl ring system (where at least one of the rings in the bicyclic system is aromatic) where the monocyclic ring and at least one of the rings in the bicyclic ring system contains one, two, three, four, or five heteroatom(s) selected from nitrogen, oxygen, phosphorous, and sulfur. The ring containing the heteroatom can be aromatic or non-aromatic. Representative examples include pyridinyl, imidazolyl, pyrimidinyl, pyrazolyl, triazolyl, pyrazinyl, tetrazolyl, furyl, thienyl, isoxazolyl, thiazolyl, oxazolyl, isothiazolyl, pyrrolyl, quinolinyl, isoquinolinyl, indolyl, benzimidazolyl, benzdioxolyl, benzofuranyl, cinnolinyl, indazolyl, indolizinyl, phthalazinyl, pyridazinyl, triazinyl, isoindolyl, pteridinyl, purinyl, oxadiazolyl, triazolyl, thiadiazolyl, thiadiazolyl, furazanyl, benzofurazanyl, benzothiophenyl, benzothiazolyl, benzoxazolyl, quinazolinyl, quinoxalinyl, naphthyridinyl, and furopyridinyl. Fused, bridged, and spiro moieties are also included within the scope of this definition. In another embodiment, the heteroaryl groups described herein are selected from thienyl, oxazolyl, furyl, pyrrolyl, imidazolyl, thiazolyl, pyridinyl, imidazolyl and pyrimidinyl. [00124] "Carbonyl" refers to the group "-C(O)-", which is bivalent.
[00125] "Aminocarbonyl" refers to the group "-C(O)-NH2 " wherein the parent moiety is attached to the amino group.
[00126] "Alkoxycarbonyl" refers to the group "-C(O)alkoxy," wherein alkoxy is as defined above, and the parent moiety is attached to the carbonyl. A non-limiting example includes -C(O)-OC(CH3)3.
[00127] "Hydroxyalkynyl" refers to a group wherein the parent moiety is attached to the alkynyl group, as defined above, and a hydroxyl group is attached to the alkynyl. A non- limiting example includes 4-hydroxybut-l-yn-l-yl. [00128] "Hydroxyalkyl" refers to a group wherein the paret moiety is attached to the alkyl group, and a hydroxyl group is attached to the alkyl, wherein the alkyl portion is as defined in the term "alkyl" herein [00129] "Amino(imino)alkyl" refers to a group represented by -alkyl-C(=NH)-
NH2, wherein the alkyl portion is as defined in the term "alkyl" herein. A non- limiting example includes amino(imino)methyl.
[00130] "Dihydroxyalkyl" refers to a group wherein the parent moiety is attached to the alkyl group, and a two hydroxyl groups are attached to the alkyl, wherein the alkyl portion is as defined in the term "alkyl" herein. [00131] "Alkylaminoalkyl" refers to -(alkyl)NH(alkyl), wherein the alkyl portion is as defined in the term "alkyl" herein.
[00132] "Alkylaminoalkylamino" refers to -N(H)(alkyl)NH(alkyl), wherein the alkyl portion is as defined in the term "alkyl" herein.
[00133] "Aminoalkylamino" refers to -N(H)(alky I)NH2, wherein the alkyl portion is as defined in the term "alkyl" herein. [00134] "Arylalkylamino" refers to -N(H)(alkyl)aryl, wherein the "alkyl" and
"aryl" portions are as defined in the terms "alkyl" and "aryl" herein. [00135] "Alkylsulfonylheterocycloalkylamino" refers to -N(H)-heterocycloalkyl-
S(O)2-alkyl, wherein the amino is attached to the parent moiety, wherein the "alkyl" and "heterocycloalkyl" portions are as defined herein. [00136] "Cycloalkylalkylamino" refers to -N(H)-alkylcycloalkyl, wherein the amino is attached to the parent moiety, and wherein the "alkyl" and "cycloalkyl" portions are as defined herein .
[00137] "Dialkylaminoalkoxy" refers to -(alkoxy)N(alkyl)2, wherein the "alkoxy" and "alkyl" portions are as defined herein. One such non-limiting example of
"dialkylaminoalkoxy" includes dimethylaminoethyloxy represented by -O-
(CH2)2N(CH3)2. [00138] "Alkylsulfonylalkylamino" refers to -NH2-alkyl-S(O)2-alkyl, wherein the amino portion of this group is attached to the parent moieity, and wherein the "alkyl" portions are as defined above. A non-limiting example includes methy lsulfony lethy lamino .
[00139] "Aminocarbonylalkylaminoalkyl" refers to the group "-alkyl-N(H)-alkyl- C(O)-NH2" wherein the parent moiety is attached to the alkyl group, and wherein the alkyl portions are as defined herein. [00140] "Aminoalkylaminoalkyl" refers to the group "-alkyl-N(H)-alkyl-NH2" wherein the parent moiety is attached to the alkyl group, and wherein the alkyl portions are as defined herein.
[00141] "Dialkylaminoalkylaminoalkyl" refers to the group -alkyl-N(H)-alkyl- N(alkyl)2, wherein the parent moiety is attached to the alkyl group, and wherein the
"alkyl" portions are as defined herein. [00142] "Carboxyalkylaminoalkyl" refers to the group -alkyl-N(H)-alkyl-
C(O)OH, wherein the parent moiety is attached to the alkyl group, and wherein the
"alkyl" portions are as defined herein. [00143] "Cycloakylaminoalkyl" refers to the group -alkyl-N(H)-alkyl-cycloalkyl, wherein the parent moiety is attached to the alkyl group, and wherein the "alkyl" and cycloalkyl portions are as defined herein. [00144] "Dialkylaminoarylalkylaminoalkyl" refers to the group -alkyl-N(H)-alkyl- aryl-N(alkyl)2, wherein the parent moiety is attached to the alkyl group, and wherein the "alkyl" and "aryl" portions are as defined herein.
[00145] "Heteroarylalkylaminoalkyl" refers to the group -alkyl-N(H)-alkyl- heteroaryl, wherein the parent moiety is attached to the alkyl group, and wherein the
"alkyl" and "heteroaryl" portions are as defined herein.
[00146] "Heterocycloalkylarylalkylaminoalkyl" refers to the group -alkyl-N(H)- alkyl-aryl-heterocycloalkyl, wherein the parent moiety is attached to the alkyl group, and wherein the "alkyl" and "heterocycloalkyl" portions are as defined herein. [00147] "Alkoxyalkylaminoalkyl" refers to the group -alkyl-N(H)-alkyl-O-alkyl, wherein the parent moiety is attached to the alkyl group, and wherein the "alkyl" portions are as defined herein. [00148] "Hydroxymethylalkynyl" refers to the group -alkynyl-CH2-OH, wherein the parent moiety is attached to the alkynyl group, and wherein the "alkynyl" portion is as defined herein. [00149] "Heteroarylalkyl" refers to the group -alkyl-heteroaryl, wherein the parent moiety is attached to the alkyl group, and wherein the "alkyl" and heteroaryl portions are as defined herein.
[00150] "Heterocycloalkylaminoalkyl" refers to the group -alkyl-N(H)-alkyl- heterocylcoalkyl, wherein the parent moiety is attached to the alkyl group, and wherein the "alkyl" and "heterocycloalkyl" portions are as defined herein. [00151] "Cycloalkylaminoalkyl" refers to the group -alkyl-N(H)-cycloalkyl, wherein the parent moiety is attached to the alkyl group, and wherein the "alkyl" and
"cycloalkyl" portions are as defined herein.
[00152] "Heterocycloalkylalkylaminoalkyl" refers to the group -alkyl-N(H)-alkyl- heterocycloalkyl, wherein the parent moiety is attached to the alkyl group, and wherein the "alkyl" and "heterocycloalkyl" portions are as defined herein. [00153] "Arylalkylaminoalkyl" refers to the group -alkyl-N(H)-alkyl-aryl, wherein the parent moiety is attached to the alkyl group, and wherein the "alkyl" and "aryl" portions are as defined herein. [00154] "Alkylheterocycloalkyl" refers to the group -alkyl-heterocycoalkyl, wherein the parent moiety is attached to the alkyl group, and wherein the "alkyl" and
"heterocycloalkyl" portions are as defined herein. [00155] "Heteroarylaminoalkyl" refers to the group -alkyl-N(H)-heteroaryl, wherein the parent moiety is attached to the alkyl group, and wherein the "alkyl" and "heteroaryl" portions are as defined herein.
[00156] "Arylamino" refers to the group -N(H)-aryl, wherein the parent moiety is attached to the amino group, and wherein the "aryl" portion is as defined herein. [00157] "Aryloxyalkyl" refers to the group -alkyl-O-aryl, wherein the parent moiety is attached to the alkyl group, and wherein the "alkyl" and "aryl" portions are as defined herein.
[00158] "Heteroarylalkyl" refers to the group -alkyl-heteroaryl, wherein the parent moiety is attached to the alkyl group, and wherein the " alkyl" and "heteroaryl" portions are as defined herein.
[00159] Alkylpiperazinylcarbonyl" refers to the group -C(O)-piperazinyl-alkyl, wherein the parent moiety is attached to the carbonyl group, and wherein the "alkyl" portion is as defined herein. [00160] "Alkylheterocycloakylarylalkoxyalkyl" refers to the group -alkyl-O- alkyl-aryl-heterocycloalkyl-alkyl, wherein the parent moiety is attached to the alkyl group, and wherein the "aryl," "aryl" and "heterocycloalkyl" portions are as defined herein.
[00161] "Alkylheteroarylalkoxyalkyl" refers to the group -alkyl-O-alkyl- heteroaryl-alkyl, wherein the parent moiety is attached to the alkyl group, and wherein the "aryl" and "heteroaryl" portions are as defined herein. [00162] "Dialkylaminoalkylamino" refers to the group -N(H)-alkyl-N(alkyl)2, wherein the parent moiety is attached to the amino group, and wherein the "alkyl" portions are as defined herein.
[00163] "Heterocycloalkylalkylamino" refers to the group -N(H)-alkyl- heterocycloalkyl, wherein the parent moiety is attached to the amino group, and wherein the "alkyl" and "heterocycloalkyl" portions are as defined herein. [00164] "Heteroarylamino" refers to the group -N(H)-heteroaryl, wherein the parent moiety is attached to the amino group, and wherein the "heteroaryl" portion is as defined herein. [00165] "Heterocycloalkylalkoxyalkyl" refers to the group -alkyl-O-alkyl- heterocycloalkyl, wherein the parent moiety is attached to the alkyl group, and wherein the "alkyl" and heterocycloalkyl portions are as defined herein. [00166] "Carboxyalkyl" refers to the group -alkyl-C(O)-OH, wherein the parent moiety is attached to the alkyl group, and wherein the "alkyl" portion is as defined herein.
[00167] "Heterocycloalkyloxyalkyl" refers to the group -alkyl-O- heterocycloalkyl, wherein the parent moiety is attached to the alkyl group, and wherein the "alkyl" and "heterocycloalkyl" portions are as defined herein. [00168] "Alkoxycarbonylalkyl" refers to the group -alkyl-C(O)-O-alkyl, wherein the parent moiety is attached to the alkyl group, and wherein the "alkyl" portions are as defined herein. [00169] "Arylalkoxy" refers to the group -alkyl-O-aryl, wherein the parent moiety is attached to the alkyl group, and wherein the "alkyl" and "aryl" portions are as defined herein. [00170] "Spiro-heterocycloalkyl" refers to the group:
Figure imgf000126_0001
wherein H represents a heterocycloalkyl group and P represents the parent moiety, wherein the heterocycloalkyl portion is as defined herein. [00171] "Arylalkylaminocarbonyl" refers to the group -C(O)-N(H)-alkyl-aryl, wherein the parent moiety is attached to the carbonyl group, and wherein the "alkyl" and "aryl" portions are as defined herein.
[00172] "Heterocycloalkylalkylamino" refers to the group -N(H)-alkyl- heterocycloalkyl, wherein the parent moiety is attached to the amino group, and wherein the "alkyl" and "heterocycloalkyl" portions are as defined herein. [00173] "Dialkylaminoalkylcarbonyl" refers to the group -C(O)-alkyl-N(alkyl)2, wherein the parent moiety is attached to the carbonyl group, and wherein the "alkyl" portions are as defined herein. [00174] "Dialkylaminocarbonylalkyl"refers to the group -alkyl-C(O)-N(alkyl)2, wherein the parent moiety is attached to the alkyl group, and wherein the "alkyl" portions are as defined herein. [00175] "Heterocycloalkylcarbonyl" refers to the group -C(O)-heterocycloalkyl, wherein the parent moiety is attached to the carbonyl group, and wherein the "heterocycloalkyl" portion is as defined herein.
[00176] "Alkylthio" refers to the group -S-alkyl, wherein the parent moiety is attached to the thio group (-S-), and wherein the "alkyl" portion is as defined herein. [00177] "Alkylsulfonyl" refers to the group -S(O)2-alkyl, wherein the parent moiety is attached to the sulfonyl group [-S(O)2-], and wherein the "alkyl" portion is as defined herein
[00178] "Alkylheterocycloalkyl" refers to the group -alkyl-heterocycloalkyl, wherein the parent moiety is attached to the alkyl group, and wherein the "alkyl" and
"heterocycloalkyl" portions are as defined herein.
[00179] "Alkoxycarbonylheterocycloalkylaminoalkyl" refers to the group -alkyl- N(H)-heterocycloalkyl-C(O)-O-alkyl, wherein the parent moiety is attached to the alkyl group, and wherein the "alkyl" and "heterocycloalkyl" portions are as defined herein. [00180] "Heterocycloalkylaminoalkyl" refers to the group -alkyl-N(H)- heterocycloalkyl, wherein the parent moiety is attached to the alkyl group, and wherein the "alkyl" and "heterocycloalkyl" portions are as defined herein.
[00181] "Aminoalkylaminocarbonyl" refers to the group -N(H)-alkyl- heterocycloalkyl, wherein the parent moiety is attached to the amino group, and wherein the "alkyl" and "heterocycloalkyl" portions are as defined herein. [00182] "Heteroarylcarbonyl" refers to the group -C(O)-heteroaryl, wherein the parent moiety is attached to the carbonyl group, and wherein the "heteroaryl" portion is defined herein.
[00183] "Arylalkylcarbonyl" refers to the group -C(O)-alkyl-aryl, wherein the parent moiety is attached to the carbonyl group, and wherein the "alkyl" and "aryl" portions are as defined herein. [00184] "Arylcarbonyl" refers to the group -C(O)-aryl, wherein the parent moiety is attached to the carbonyl group, and wherein the "aryl" portion is defined herein. [00185] Αlkylcarbonyl" refers to the group -C(O)-alkyl, wherein the parent moiety is attached to the carbonyl group, and wherein the "alkyl" portion is defined herein. [00186] "Alkoxyalkylcarbonyl" refers to the group -C(O)-alkyl-O-alkyl, wherein the parent moiety is attached to the carbonyl group, and wherein the "alkyl" portions are as defined herein. [00187] "Spiro-cycloalkyl" refers to the group:
Figure imgf000128_0001
wherein Cyc represents a cycloalkyl group and P represents the parent moiety, wherein the cycloalkyl portion is as defined herein.
[00188] When a portion of term (such as the "alkyl" portion of "arylalkyl") is referred to as being defined above or defined herein, this means that this portion has the same meaning as the definition of this term within this specification. [00189] The phrases "the compounds in this disclosure," the compounds in the disclosure, the compounds disclosed herein, compounds of this disclosure, and similar phrases that contain both of the words "compounds" and "disclosure" are meant to mean compounds of Formula I and all of the embodiments of Formula I described herein.
[00190] In the case where there is a point of attachment for a monovalent substituent, such as -CH3, -NH2, or -OH, the indication of where the point of attachment is is not necessary. That is, -CH3 has the same meaning as CH3, -NH2 has the same meaning as NH2, and -OH has the same meaning as OH. [00191] In Table 1 , where there appears to be an empty valence for oxygen or nitrogen for any of the compounds listed in this table, where the name of the structure requires that the empty valence is filled with hydrogen, it is assumed that the missing valence is filled with hydrogen for each of these cases. [00192] When a group is referred to as "-(Ci-Ce)alkyl heterocyclyl" the heterocyclyl is attached to a parent structure via an alkyl group.
[00193] "Optional" or "optionally" means that the subsequently described event or circumstance can or can not occur, and that the description includes instances where said event or circumstance occurs and instances in which it does not. One of ordinary skill in the art would understand that with respect to any molecule described as containing one or more optional substituents, only sterically practical and/or synthetically feasible compounds are meant to be included. "Optionally substituted" means substituted or unsubstituted and refers to all subsequent modifiers in a term unless otherwise specified. So, for example, in the term "optionally substituted arylalkyl," both the "alkyl" portion and the "aryl" portion of the molecule can be substituted or unsubstituted.
[00194] Unless otherwise specified, the term "optionally substituted" applies to the chemical moiety immediately preceding it. For instance, if a variable group (such as R) is defined as aryl, optionally substituted alkyl, or cycloalkyl, then only the alkyl group is optionally substituted.
[00195] "Saturated bridged ring system" refers to a bicyclic or polycyclic ring system that is not aromatic. Such a system can contain isolated or conjugated unsaturation, but not aromatic or heteroaromatic rings in its core structure (but can have aromatic substitution thereon). For example, hexahydro-furo[3,2-b]furan, 2,3,3a,4,7,7a-hexahydro-lH-indene, 7-aza-bicyclo[2.2.1]-heptane, and l,2,3,4,4a,5,8,8a-octahydro-naphthalene are all included in the class "saturated bridged ring system.
[00196] "Spirocyclyl" or "spirocyclic ring" refers to a ring originating from a particular annular carbon of another ring. For example, as depicted below, a ring atom of a saturated bridged ring system (rings B and B'), but not a bridgehead atom, can be a shared atom between the saturated bridged ring system and a spirocyclyl (ring A) attached thereto. A spirocyclyl can be carbocyclic or heteroalicyclic.
Figure imgf000130_0001
[00197] Some of the compounds of the disclosure can have imino, amino, oxo or hydroxy substituents off aromatic heterocyclyl systems. For purposes of this disclosure, it is understood that such imino, amino, oxo or hydroxy substituents can exist in their corresponding tautomeric form, i.e., amino, imino, hydroxy or oxo, respectively.
[00198] "Animal" for the purposes of this disclosure includes humans (including patients receiving treatment) and other animals, particularly mammals, and other organisms. Thus, the methods are applicable to both human therapy and veterinary applications. In a preferred embodiment the patient is a mammal, and in a most preferred embodiment the patient is human.
[00199] "Kinase-dependent diseases or conditions" refer to pathologic conditions that depend on the activity of one or more protein kinases. Kinases either directly or indirectly participate in the signal transduction pathways of a variety of cellular activities including proliferation, adhesion, migration, differentiation and invasion. Diseases associated with kinase activities include tumor growth, the pathologic neovascularization that supports solid tumor growth, and associated with other diseases where excessive local vascularization is involved such as ocular diseases (diabetic retinopathy, age-related macular degeneration, and the like) and inflammation (psoriasis, rheumatoid arthritis, and the like).
[00200] While not wishing to be bound to theory, phosphatases can also play a role in "kinase-dependent diseases or conditions" as cognates of kinases; that is, kinases phosphorylate and phosphatases dephosphorylate, for example protein substrates. Therefore compounds of this disclosure, while modulating kinase activity as described herein, can also modulate, either directly or indirectly, phosphatase activity. This additional modulation, if present, can be synergistic (or not) to activity of compounds of this disclosure toward a related or otherwise interdependent kinase or kinase family. In any case, as stated previously, the compounds of this disclosure are useful for treating diseases characterized in part by abnormal levels of cell proliferation (i.e. tumor growth), programmed cell death (apoptosis), cell migration and invasion and angiogenesis associated with tumor growth.
[00201] "Therapeutically effective amount" is an amount of a compound of this disclosue, that when administered to a patient, ameliorates a symptom of the disease. The amount of a compound of this disclosure which constitutes a "therapeutically effective amount" will vary depending on the compound, the disease state and its severity, the age of the patient to be treated, and the like. The therapeutically effective amount can be determined routinely by one of ordinary skill in the art having regard to their knowledge and to this disclosure. [00202] "Cancer" as referred to in the specification and in the claims refers to cellular-pro liferative disease states, including but not limited to: Cardiac: sarcoma (angiosarcoma, fibrosarcoma, rhabdomyosarcoma, liposarcoma), myxoma, rhabdomyoma, fibroma, lipoma and teratoma; Lung: bronchogenic carcinoma (squamous cell, undifferentiated small cell, undifferentiated large cell, adenocarcinoma), alveolar (bronchiolar) carcinoma, bronchial adenoma, sarcoma, lymphoma, chondromatous hamartoma, mesothelioma; Gastrointestinal: esophagus (squamous cell carcinoma, adenocarcinoma, leiomyosarcoma, lymphoma), stomach (carcinoma, lymphoma, leiomyosarcoma), pancreas (ductal adenocarcinoma, insulinoma, glucagonoma, gastrinoma, carcinoid tumors, vipoma), small bowel (adenocarcinoma, lymphoma, carcinoid tumors, Karposi's sarcoma, leiomyoma, hemangioma, lipoma, neurofibroma, fibroma), large bowel (adenocarcinoma, tubular adenoma, villous adenoma, hamartoma, leiomyoma); Genitourinary tract: kidney (adenocarcinoma, Wilm's tumor [nephroblastoma], lymphoma, leukemia), bladder and urethra (squamous cell carcinoma, transitional cell carcinoma, adenocarcinoma), prostrate (adenocarcinoma, sarcoma), testis (seminoma, teratoma, embryonal carcinoma, teratocarcinoma, choriocarcinoma, sarcoma, interstitial cell carcinoma, fibroma, fibroadenoma, adenomatoid tumors, lipoma); Liver: hepatoma (hepatocellular carcinoma), cholangiocarcinoma, hepatoblastoma, angiosarcoma, hepatocellular adenoma, hemangioma; Bone: osteogenic sarcoma (osteosarcoma), fibrosarcoma, malignant fibrous histiocytoma, chondrosarcoma, Ewing's sarcoma, malignant lymphoma (reticulum cell sarcoma), multiple myeloma, malignant giant cell tumor chordoma, osteochronfroma (osteocartilaginous exostoses), benign chondroma, chondroblastoma, chondromyxofϊbroma, osteoid osteoma and giant cell tumors; Nervous system: skull (osteoma, hemangioma, granuloma, xanthoma, osteitis deformans), meninges (meningioma, meningiosarcoma, gliomatosis), brain (astrocytoma, medulloblastoma, glioma, ependymoma, germinoma [pinealoma], glioblastoma multiform, oligodendroglioma, schwannoma, retinoblastoma, congenital tumors), spinal cord neurofibroma, meningioma, glioma, sarcoma); Gynecological: uterus (endometrial carcinoma), cervix (cervical carcinoma, pre -tumor cervical dysplasia), ovaries (ovarian carcinoma [serous cystadenocarcinoma, mucinous cystadenocarcinoma, unclassified carcinoma], granulosa-thecal cell tumors, Sertoli- Leydig cell tumors, dysgerminoma, malignant teratoma), vulva (squamous cell carcinoma, intraepithelial carcinoma, adenocarcinoma, fibrosarcoma, melanoma), vagina (clear cell carcinoma, squamous cell carcinoma, botryoid sarcoma (embryonal rhabdomyosarcoma], fallopian tubes (carcinoma); Hematologic: blood (myeloid leukemia [acute and chronic], acute lymphoblastic leukemia, chronic lymphocytic leukemia, myeloproliferative diseases, multiple myeloma, myelodysplastic syndrome), Hodgkin's disease, non-Hodgkin's lymphoma [malignant lymphoma]; Skin: malignant melanoma, basal cell carcinoma, squamous cell carcinoma, Karposi's sarcoma, moles dysplastic nevi, lipoma, angioma, dermatofibroma, keloids, psoriasis; and Adrenal glands: neuroblastoma. Thus, the term "cancerous cell" as provided herein, includes a cell afflicted by any one of the above-identified conditions. [00203] A "pharmaceutically acceptable salt" of a compound means a salt that is pharmaceutically acceptable and that possesses the desired pharmacological activity of the parent compound. It is understood that the pharmaceutically acceptable salts are non-toxic. Additional information on suitable pharmaceutically acceptable salts can be found in Remington 's Pharmaceutical Sciences, 17th ed., Mack Publishing Company, Easton, PA, 1985, which is incorporated herein by reference or S. M. Berge, et al, "Pharmaceutical Salts," J. Pharm. ScL, 1977;66:1-19 both of which are incorporated herein by reference.
[00204] Examples of pharmaceutically acceptable acid addition salts include those formed with inorganic acids such as hydrochloric acid, hydrobromic acid, sulfuric acid, nitric acid, phosphoric acid, and the like; as well as organic acids such as acetic acid, trifluoroacetic acid, propionic acid, hexanoic acid, cyclopentanepropionic acid, glycolic acid, pyruvic acid, lactic acid, oxalic acid, maleic acid, malonic acid, succinic acid, fumaric acid, tartaric acid, citric acid, benzoic acid, cinnamic acid, 3-(4- hydroxybenzoyl)benzoic acid, mandelic acid, methanesulfonic acid, ethanesulfonic acid, 1 ,2-ethanedisulfonic acid, 2-hydroxyethanesulfonic acid, benzenesulfonic acid,
4-chlorobenzenesulfonic acid, 2-naphthalenesulfonic acid, 4-toluenesulfonic acid, camphorsulfonic acid, glucoheptonic acid, 4,4'-methylenebis-(3-hydroxy-2-ene-l- carboxylic acid), 3-phenylpropionic acid, trimethylacetic acid, tertiary butylacetic acid, lauryl sulfuric acid, gluconic acid, glutamic acid, hydroxynaphthoic acid, salicylic acid, stearic acid, muconic acid, p-toluenesulfonic acid, and salicylic acid and the like.
[00205] Examples of a pharmaceutically acceptable base addition salts include those formed when an acidic proton present in the parent compound is replaced by a metal ion, such as sodium, potassium, lithium, ammonium, calcium, magnesium, iron, zinc, copper, manganese, aluminum salts and the like. Preferable salts are the ammonium, potassium, sodium, calcium, and magnesium salts. Salts derived from pharmaceutically acceptable organic non-toxic bases include, but are not limited to, salts of primary, secondary, and terriary amines, substituted amines including naturally occurring substituted amines, cyclic amines and basic ion exchange resins. Examples of organic bases include isopropylamine, trimethylamine, diethylamine, triethylamine, tripropylamine, ethanolamine, 2-dimethylaminoethanol, 2- diethylaminoethanol, dicyclohexylamine, lysine, arginine, histidine, caffeine, procaine, hydrabamine, choline, betaine, ethylenediamine, glucosamine, methylglucamine, theobromine, purines, piperazine, piperidine, N-ethylpiperidine, tromethamine, JV-methylglucamine, polyamine resins, and the like. Exemplary organic bases are isopropylamine, diethylamine, ethanolamine, trimethylamine, dicyclohexylamine, choline, and caffeine. [00206] "Prodrug" refers to compounds that are transformed (typically rapidly) in vivo to yield the parent compound of the above formulae, for example, by hydrolysis in blood. Common examples include, but are not limited to, ester and amide forms of a compound having an active form bearing a carboxylic acid moiety. Examples of pharmaceutically acceptable esters of the compounds of this disclosure include, but are not limited to, alkyl esters (for example with between about one and about six carbons) the alkyl group is a straight or branched chain. Acceptable esters also include cycloalkyl esters and arylalkyl esters such as, but not limited to benzyl. Examples of pharmaceutically acceptable amides of the compounds of this disclosure include, but are not limited to, primary amides, and secondary and tertiary alkyl amides (for example with between about one and about six carbons). Amides and esters of the compounds of this disclosure can be prepared according to conventional methods. A thorough discussion of prodrugs is provided in T. Higuchi and V. Stella, "Pro-drugs as Novel Delivery Systems," Vol. 14 of the A.C.S. Symposium Series, and in
Bioreversible Carriers in Drug Design, ed. Edward B. Roche, American
Pharmaceutical Association and Pergamon Press, 1987, both of which are incorporated herein by reference for all purposes.
[00207] "Metabolite" refers to the break-down or end product of a compound or its salt produced by metabolism or biotransformation in the animal or human body; for example, biotransformation to a more polar molecule such as by oxidation, reduction, or hydrolysis, or to a conjugate (see Goodman and Gilman, "The Pharmacological Basis of Therapeutics" 8.sup.th Ed., Pergamon Press, Gilman et al.. (eds), 1990 for a discussion of biotransformation). As used herein, the metabolite of a compound of this disclosure or its salt can be the biologically active form of the compound in the body. In one example, a prodrug can be used such that the biologically active form, a metabolite, is released in vivo. In another example, a biologically active metabolite is discovered serendipitously, that is, no prodrug design per se was undertaken. An assay for activity of a metabolite of a compound of this disclosure is known to one of skill in the art in light of the present disclosure.
[00208] The compounds of this disclosure also include N-oxide derivatives and protected derivatives of compounds of Formula I, II or III. For example, when compounds of Formula I contain an oxidizable nitrogen atom, the nitrogen atom can be converted to an N-oxide by methods well known in the art. When compounds of Formula I contain groups such as hydroxy, carboxy, thiol or any group containing a nitrogen atom(s), these groups can be protected with a suitable "protecting group" or "protective group". A comprehensive list of suitable protective groups can be found in T.W. Greene, Protective Groups in Organic Synthesis, John Wiley & Sons, Inc. 1991, the disclosure of which is incorporated herein by reference in its entirety. The protected derivatives of compounds of Formula I can be prepared by methods well known in the art.
[00209] "Treating" or "treatment" of a disease, disorder, or syndrome, as used herein, includes (i) preventing the disease, disorder, or syndrome from occurring in a human, i.e. causing the clinical symptoms of the disease, disorder, or syndrome not to develop in an animal that can be exposed to or predisposed to the disease, disorder, or syndrome but does not yet experience or display symptoms of the disease, disorder, or syndrome; (ii) inhibiting the disease, disorder, or syndrome, i.e., arresting its development; and (iii) relieving the disease, disorder, or syndrome, i.e., causing regression of the disease, disorder, or syndrome. As is known in the art, adjustments for systemic versus localized delivery, age, body weight, general health, sex, diet, time of administration, drug interaction and the severity of the condition can be necessary, and will be ascertainable with routine experimentation by one of ordinary skill in the art.
[00210] One of ordinary skill in the art would understand that certain crystallized, protein-ligand complexes, in particular HSP90-ligand complexes, and their corresponding x-ray structure coordinates can be used to reveal new structural information useful for understanding the biological activity of kinases as described herein. As well, the key structural features of the aforementioned proteins, particularly, the shape of the ligand binding site, are useful in methods for designing or identifying selective modulators of kinases and in solving the structures of other proteins with similar features. Such protein-ligand complexes, having compounds of this disclosure as their ligand component, are an aspect of this disclosure. [00211] As well, one of ordinary skill in the art would appreciate that such suitable x-ray quality crystals can be used as part of a method of identifying a candidate agent capable of binding to and modulating the activity of kinases. Such methods can be characterized by the following aspects: a) introducing into a suitable computer program, information defining a ligand binding domain of a kinase in a conformation (e.g. as defined by x-ray structure coordinates obtained from suitable x-ray quality crystals as described above) wherein the computer program creates a model of the three dimensional structures of the ligand binding domain, b) introducing a model of the three dimensional structure of a candidate agent in the computer program, c) superimposing the model of the candidate agent on the model of the ligand binding domain, and d) assessing whether the candidate agent model fits spatially into the ligand binding domain. Aspects a-d are not necessarily carried out in the aforementioned order. Such methods can further entail: performing rational drug design with the model of the three-dimensional structure, and selecting a potential candidate agent in conjunction with computer modeling.
[00212] Additionally, one skilled in the art would appreciate that such methods can further entail: employing a candidate agent, so-determined to fit spatially into the ligand binding domain, in a biological activity assay for kinase modulation, and determining whether said candidate agent modulates kinase activity in the assay. Such methods can also include administering the candidate agent, determined to modulate kinase activity, to a mammal suffering from a condition treatable by kinase modulation, such as those described above. [00213] Also, one skilled in the art would appreciate that compounds disclosed herein can be used in a method of evaluating the ability of a test agent to associate with a molecule or molecular complex comprising a ligand binding domain of a kinase. Such a method can be characterized by the following aspects: a) creating a computer model of a kinase binding pocket using structure coordinates obtained from suitable x-ray quality crystals of the kinase, b) employing computational algorithms to perform a fitting operation between the test agent and the computer model of the binding pocket, and c) analyzing the results of the fitting operation to quantify the association between the test agent and the computer model of the binding pocket.
General Administration [00214] In certain other preferred embodiments, administration can preferably be by the oral route. Administration of the compounds of this disclosure, or their pharmaceutically acceptable salts, in pure form or in an appropriate pharmaceutical composition, can be carried out via any of the accepted modes of administration or agents for serving similar utilities. Thus, administration can be, for example, orally, nasally, parenterally (intravenous, intramuscular, or subcutaneous), topically, transdermally, intravaginally, intravesically, intracistemally, or rectally, in the form of solid, semi-solid, lyophilized powder, or liquid dosage forms, such as for example, tablets, suppositories, pills, soft elastic and hard gelatin capsules, powders, solutions, suspensions, or aerosols, or the like, preferably in unit dosage forms suitable for simple administration of precise dosages.
[00215] The compositions will include a conventional pharmaceutical carrier or excipient and a compound of this disclosure as the/an active agent, and, in addition, can include carriers and adjuvants, etc. [00216] Adjuvants include preserving, wetting, suspending, sweetening, flavoring, perfuming, emulsifying, and dispensing agents. Prevention of the action of microorganisms can be ensured by various antibacterial and antifungal agents, for example, parabens, chlorobutanol, phenol, sorbic acid, and the like. It can also be desirable to include isotonic agents, for example sugars, sodium chloride, and the like.
Prolonged absorption of the injectable pharmaceutical form can be brought about by the use of agents delaying absorption, for example, aluminum monostearate and gelatin.
[00217] If desired, a pharmaceutical composition of the compounds in this disclosure can also contain minor amounts of auxiliary substances such as wetting or emulsifying agents, pH buffering agents, antioxidants, and the like, such as, for example, citric acid, sorbitan monolaurate, triethanolamine oleate, butylalted hydroxytoluene, etc.
[00218] The choice of formulation depends on various factors such as the mode of drug administration (e.g., for oral administration, formulations in the form of tablets, pills or capsules are preferred) and the bioavailability of the drug substance. Recently, pharmaceutical formulations have been developed especially for drugs that show poor bioavailability based upon the principle that bioavailability can be increased by increasing the surface area i.e., decreasing particle size. For example, U.S. Pat. No. 4,107,288 describes a pharmaceutical formulation having particles in the size range from 10 to 1,000 nm in which the active material is supported on a crosslinked matrix of macromolecules. U.S. Pat. No. 5,145,684 describes the production of a pharmaceutical formulation in which the drug substance is pulverized to nanoparticles (average particle size of 400 nm) in the presence of a surface modifier and then dispersed in a liquid medium to give a pharmaceutical formulation that exhibits remarkably high bioavailability.
[00219] Compositions suitable for parenteral injection can comprise physiologically acceptable sterile aqueous or nonaqueous solutions, dispersions, suspensions or emulsions, and sterile powders for reconstitution into sterile injectable solutions or dispersions. Examples of suitable aqueous and nonaqueous carriers, diluents, solvents or vehicles include water, ethanol, polyols (propyleneglycol, polyethyleneglycol, glycerol, and the like), suitable mixtures thereof, vegetable oils (such as olive oil) and injectable organic esters such as ethyl oleate. Proper fluidity can be maintained, for example, by the use of a coating such as lecithin, by the maintenance of the required particle size in the case of dispersions and by the use of surfactants.
[00220] One preferable route of administration is oral, using a convenient daily dosage regimen that can be adjusted according to the degree of severity of the disease- state to be treated.
[00221] Solid dosage forms for oral administration include capsules, tablets, pills, powders, and granules. In such solid dosage forms, the active compound is admixed with at least one inert customary excipient (or carrier) such as sodium citrate or dicalcium phosphate or (a) fillers or extenders, as for example, starches, lactose, sucrose, glucose, mannitol, and silicic acid, (b) binders, as for example, cellulose derivatives, starch, alignates, gelatin, polyvinylpyrrolidone, sucrose, and gum acacia, (c) humectants, as for example, glycerol, (d) disintegrating agents, as for example, agar-agar, calcium carbonate, potato or tapioca starch, alginic acid, croscarmellose sodium, complex silicates, and sodium carbonate, (e) solution retarders, as for example paraffin, (f) absorption accelerators, as for example, quaternary ammonium compounds, (g) wetting agents, as for example, cetyl alcohol, and glycerol monostearate, magnesium stearate and the like (h) adsorbents, as for example, kaolin and bentonite, and (i) lubricants, as for example, talc, calcium stearate, magnesium stearate, solid polyethylene glycols, sodium lauryl sulfate, or mixtures thereof. In the case of capsules, tablets, and pills, the dosage forms can also comprise buffering agents.
[00222] Solid dosage forms, as described above, can be prepared with coatings and shells, such as enteric coatings and others well known in the art. They can contain pacifying agents, and can also be of such composition that they release the active compound or compounds in a certain part of the intestinal tract in a delayed manner. Examples of embedded compositions that can be used are polymeric substances and waxes. The active compounds can also be in microencapsulated form, if appropriate, with one or more of the above-mentioned excipients. [00223] Liquid dosage forms for oral administration include pharmaceutically acceptable emulsions, solutions, suspensions, syrups, and elixirs. Such dosage forms are prepared, for example, by dissolving, dispersing, etc., a compound(s) of this disclosure, or a pharmaceutically acceptable salt thereof, and optional pharmaceutical adjuvants in a carrier, such as, for example, water, saline, aqueous dextrose, glycerol, ethanol and the like; solubilizing agents and emulsifiers, as for example, ethyl alcohol, isopropyl alcohol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propyleneglycol, 1,3-butyleneglycol, dimethylformamide; oils, in particular, cottonseed oil, groundnut oil, corn germ oil, olive oil, castor oil and sesame oil, glycerol, tetrahydrofurfuryl alcohol, polyethyleneglycols and fatty acid esters of sorbitan; or mixtures of these substances, and the like, to thereby form a solution or suspension.
[00224] Suspensions, in addition to the active compounds, can contain suspending agents, as for example, ethoxylated isostearyl alcohols, polyoxyethylene sorbitol and sorbitan esters, microcrystalline cellulose, aluminum metahydroxide, bentonite, agar- agar and tragacanth, or mixtures of these substances, and the like. [00225] Compositions for rectal administrations are, for example, suppositories that can be prepared by mixing the compounds of this disclosure with, for example, suitable non-irritating excipients or carriers such as cocoa butter, polyethyleneglycol or a suppository wax, which are solid at ordinary temperatures but liquid at body temperature and therefore, melt while in a suitable body cavity and release the active component therein.
[00226] Dosage forms for topical administration of a compound of this disclosure include ointments, powders, sprays, and inhalants. The active component is admixed under sterile conditions with a physiologically acceptable carrier and any preservatives, buffers, or propellants as can be required. Ophthalmic formulations, eye ointments, powders, and solutions are also contemplated for the comounds in this disclosure. [00227] Compressed gases can be used to disperse a compound of this disclosure in aerosol form. Inert gases suitable for this purpose are nitrogen, carbon dioxide, etc.
[00228] Generally, depending on the intended mode of administration, the pharmaceutically acceptable compositions will contain about 1% to about 99% by weight of a compound(s) of this disclosure, or a pharmaceutically acceptable salt thereof, and 99% to 1% by weight of a suitable pharmaceutical excipient. In one example, the composition will be between about 5% and about 75% by weight of a compound(s) of this disclosure, or a pharmaceutically acceptable salt thereof, with the rest being suitable pharmaceutical excipients. [00229] Actual methods of preparing such dosage forms are known, or will be apparent, to those skilled in this art; for example, see Remington's Pharmaceutical
Sciences, 18th Ed., (Mack Publishing Company, Easton, Pa., 1990). The composition to be administered will, in any event, contain a therapeutically effective amount of a compound of this disclosure, or a pharmaceutically acceptable salt thereof, for treatment of a disease-state in accordance with the teachings of this disclosure.
[00230] The compounds of this disclosure, or their pharmaceutically acceptable salts, are administered in a therapeutically effective amount which will vary depending upon a variety of factors including the activity of the specific compound employed, the metabolic stability and length of action of the compound, the age, body weight, general health, sex, diet, mode and time of administration, rate of excretion, drug combination, the severity of the particular disease-states, and the host undergoing therapy. The compounds of this disclosure can be administered to a patient at dosage levels in the range of about 0.1 to about 1,000 mg per day. For a normal human adult having a body weight of about 70 kilograms, a dosage in the range of about 0.01 to about 100 mg per kilogram of body weight per day is an example. The specific dosage used, however, can vary. For example, the dosage can depend on a number of factors including the requirements of the patient, the severity of the condition being treated, and the pharmacological activity of the compound being used. The determination of optimum dosages for a particular patient is well known to one of ordinary skill in the art.
[00231] The compositions will include a conventional pharmaceutical carrier or excipient and a compound of this disclosure as the/an active agent, and, in addition, can include other medicinal agents and pharmaceutical agents. Compositions of the compounds in this disclosure can be used in combination with anticancer and/or other agents that are generally administered to a patient being treated for cancer, e.g. surgery, radiation and/or chemotherapeutic agent(s). Chemotherapeutic agents that can be useful for administration in combination with compounds of Formula I in treating cancer include alkylating agents, platinum containing agents. [00232] If formulated as a fixed dose, such combination products employ the compounds of this disclosure within the dosage range described above and the other pharmaceutically active agent(s) within its approved dosage range. Compounds of this disclosure can alternatively be used sequentially with known pharmaceutically acceptable agent(s) when a combination formulation is inappropriate. [00233] Representative pharmaceutical formulations containing the compounds disclosed herein are described below. Synthetic Procedures
[00234] The compounds disclosed herein, or their pharmaceutically acceptable salts, can have asymmetric carbon atoms, oxidized sulfur atoms or quaternized nitrogen atoms in their structure. [00235] The compounds disclosed herein and their pharmaceutically acceptable salts can exist as single stereoisomers, racemates, and as mixtures of enantiomers and diastereomers. The compounds disclosed herein can also exist as geometric isomers.
All such single stereoisomers, racemates and mixtures thereof, and geometric isomers are intended to be within the scope of the compounds disclosed herein.
[00236] It is assumed that when considering generic descriptions of compounds of the disclosed herein for the purpose of constructing a compound, such construction results in the creation of a stable structure. That is, one of ordinary skill in the art would recognize that theoretically some constructs which would not normally be considered as stable compounds (that is, sterically practical and/or synthetically feasible, supra). [00237] Methods for the preparation and/or separation and isolation of single stereoisomers from racemic mixtures or non-racemic mixtures of stereoisomers are well known in the art. For example, optically active (R)- and (S)- isomers can be prepared using chiral synthons or chiral reagents, or resolved using conventional techniques. Enantiomers (R- and S-isomers) can be resolved by methods known to one of ordinary skill in the art, for example by: formation of diastereoisomeric salts or complexes which can be separated, for example, by crystallization; via formation of diastereoisomeric derivatives which can be separated, for example, by crystallization, selective reaction of one enantiomer with an enantiomer-specific reagent, for example enzymatic oxidation or reduction, followed by separation of the modified and unmodified enantiomers; or gas-liquid or liquid chromatography in a chiral environment, for example on a chiral support, such as silica with a bound chiral ligand or in the presence of a chiral solvent. It will be appreciated that where a desired enantiomer is converted into another chemical entity by one of the separation procedures described above, a further step can be required to liberate the desired enantiomeric form. Alternatively, specific enantiomer can be synthesized by asymmetric synthesis using optically active reagents, substrates, catalysts or solvents or by converting on enantiomer to the other by asymmetric transformation. For a mixture of enantiomers, enriched in a particular enantiomer, the major component enantiomer can be further enriched (with concomitant loss in yield) by recrystallization.
[00238] In addition, the compounds of this disclosure can exist in unsolvated as well as solvated forms with pharmaceutically acceptable solvents such as water, ethanol, and the like. In general, the solvated forms are considered equivalent to the unsolvated forms for the purposes of the compounds of this disclosure.
[00239] In addition, it is intended that the present disclosure cover compounds made either using standard organic synthetic techniques, including combinatorial chemistry or by biological methods, such as bacterial digestion, metabolism, enzymatic conversion, and the like.
[00240] The examples and scheme below depict the general synthetic procedure for the compounds disclosed herein. Synthesis of the compounds disclosed herein is not limited by these examples and schemes. One skilled in the art will know that other procedures can be used to synthesize the compounds disclosed herein, and that the procedures described in the examples and schemes is only one such procedure. In the descriptions below, one of ordinary skill in the art would recognize that specific reaction conditions, added reagents, solvents, and reaction temperatures can be modified for the synthesis of specific compounds that fall within the scope of this disclosure. All intermediate compounds described below, for which there is no descripton of how to synthesize such intermediates within these examples below, are commercially available compounds unless otherwise specified.
EXAMPLES Instrumentation
[00241] IR spectra were collected by reflectance on a Perkin Elmer Spectrum™ 100 FT-IR. 1H NMR were collected on a Varian 400 MHz with Mercury and Mercury consoles.
[00242] All variables in the Examples below (e.g., R3a, Ri5, etc.) are described within the compounds within each of these examples unless otherwise specified. The variables in the examples below are not intended to change or limit the scope of the disclosure above or in the claims. When the variables within the examples below correspond to the same variables within the disclosure above or the claims (for instance, R3a), this does not intend to alter or change the meaning of the variables in this disclosure or in the claims.
EXAMPLE 1: (Scheme 1)
Scheme 1
Figure imgf000143_0001
wherein R3a is as defined in the disclosure above and R15 is described within the compounds within this example. 2-(4-bromo-2-cyanophenoxy)acetamide 2
[00243] To a solution of 5-bromo-2-hydroxybenzonitrile 1 (1O g, 50.5 mmol) in 50 mL acetone was added potassium carbonate (13.8 g, 100 mmol) and 2- bromoacetamide (6.9 g, 50.5 mmol). The reaction mixture was heated to 60 0C for 18 hours. After cooling, the solids were filtered off and dissolved in a large excess of water (1000 mL). The white solid was collected and dried to afford 11.2 g (89%) of 2- (4-bromo-2-cyanophenoxy)acetamide 2 as a white solid. 1H NMR (400 MHz, d6- DMSO): 8.03 (d, IH), 7.83 (dd, IH), 7.53 (br s, 1 H), 7.45 (br s, IH), 7.03 (d, IH), 4.7 (s, 2H).
3 -amino-5 -bromobenzofuran-2-carboxamide 3
[00244] To a solution of KOH (8.0 g, 142 mmol) in 200 mL ethanol was added 2- (4-bromo-2-cyanophenoxy)acetamide 2 (11.2 g, 44 mmol) and heated to 80 0C for an hour. After cooling down to room temperature, the reaction mixture was poured into a large excess of water and the resultant solids were filtered off and dried to afford 8.9 g (79%) of 3 -amino-5 -bromobenzofuran-2-carboxamide 3 as a white solid. 1H NMR (400 MHz, de-DMSO): 8.11 (d, IH), 7.56 (dd, IH), 7.40 (d, IH), 7.34 (br s, 2H), 6.04 (s, 2H).
5-bromo-3-(2-chloroacetamido)benzofuran-2-carboxamide 5
[00245] A solution of 3-amino-5-bromobenzofuran-2-carboxamide 3 (1.7 g, 6.72 mmol) in chloroacetyl chloride (10 rnL) was heated to 40 0C for 30 minutes. The reaction mixture was quenched with saturated aqueous NaHCO3 (100 mL) and extracted with ethyl acetate (2 x 150 mL). The combined organic phases were washed with brine, dried over Na2SO4 and concentrated in vacuo to afford 2.03 g (90%) of 5- bromo-3-(2-chloroacetamido)benzofuran-2-carboxamide 5 as a white solid. 1H NMR (400 MHz, de-DMSO): 10.75 (s, IH), 8.25 (br s, IH), 8.23 (d, IH), 8.0 (br s, IH), 7.66 (dd, IH), 7.59 (dd, IH), 4.5 (s, 2H); MS (EI) for CnH8BrClN2O3: 331 (MH+). 8-bromo-2-(chloromethyl)benzofuror3,2-dlpyrimidin-4(3H)-one 6 [00246] A solution of 5-bromo-3-(2-chloroacetamido)benzofuran-2-carboxamide 5 (2.0 g, 6.05 mmol) in 30 mL of 2N NaOH was heated to 40 0C for 20 minutes. The reaction mixture was brought to neutral pH with IN HCl and extracted with ethyl acetate (3 x 50 mL). The combined organic phases were washed with brine, dried over Na2SO4 and concentrated in vacuo. The product was purified by SiO2 flash chromatography (50:50 hexanes/ethyl acetate to 100% ethyl acetate) to afford 8- bromo-2-(chloromethyl)benzofuro[3,2-d]pyrimidin-4(3H)-one 6 (1.3 g, 63%) as a yellowish solid. 1U NMR (400 MHz, d6-DMSO): 13.4 (s, IH), 8.23 (m, IH), 7.85 (m, 2H), 4.66 (s, 2H); MS (EI) for CnH6BrClN2O2: 313 (MH+).
(Compound 2)
[00247] 8-bromo-2-(piperidin- 1 -ylmethyl)[ 1 lbenzofuro[3 ,2-dlpyrimidin-4(3H)-one To a solution of 8-bromo-2-(chloromethyl)[l]benzofuro[3,2-d]pyrimidin-4(3H)-one, 6, (100 mg, 0.319 mmol) in 5 mL anhydrous ethanol was added piperidine (100 mg, 1.18 mmol). The reaction mixture was heated to 80 0C for 16 hours, cooled down and concentrated in vacuo. Recrystallization from hot ethanol afforded 8-bromo-2- (piperidin-l-ylmethyl)[l]benzofuro[3,2-d]pyrimidin-4(3H)-one (22 mg, 20%) as a white solid. 1H NMR (400 MHz, d6-DMSO): 8.21 (m, IH), 7.81 (m, 2H), 3.50 (s, 2H), 2.50 (m, 4H, overlapped), 1.54 (m, 4H), 1.39 (m, 2H); MS (EI) for Ci6Hi6BrN3O2: 362 (MH+).
(Compound 1)
8-bromo-2-(pyrrolidin- 1 -ylmethyl)[ 11benzomro[3,2-d]pyrimidin-4(3H)-one [00248] 8-bromo-2-(pyrrolidin- 1 -ylmethyl)[ 1 ]benzofuro[3,2-d]pyrimidin-4(3H)- one was synthesized in a manner similar to Example 1, Compound 2, wherein piperidine was substituted with pyrrolidine. Purification by preparative HPLC, followed by concentration in vacuo and lyophilization afforded the title compound (112 mg, 50%) as a white solid. 1H NMR (400 MHz, d6-DMSO): 8.2 (m, IH), 7.82 (m, 2H), 3.68 (s, 2H), 2.60 (m, 4H), 1.73 (m, 4H); MS (EI) for Ci5Hi4BrN3O2: 348 (MH+).
(Compound 3)
8-bromo-2-r(4-methylpiperazin- 1 -yl)methyll [ 1 "IbenzofuroB ,2-d1pyrimidin-4(3H)-one [00249] 8-bromo-2-[(4-methylpiperazin- 1 -yl)methyl] [ 1 ]benzofuro[3 ,2- d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 1, Compound 2, wherein piperidine was substituted with N-Methylpiperazine. Purification by preparative HPLC resulting in 14 mg (18% Yield) of 8-bromo-2-[(4- methylpiperazin-l-yl)methyl][l]benzofuro[3,2-d]pyrimidin-4(3H)-one as the acetate salt. 1U NMR (400MHz, d6-DMSO + D2O): 8.26 (s, IH), 7.82 (m, 2H), 7.98 (m, IH), 4.0 (m, 4H), 3.60 (s, 2H), 2.61 (m, 4H) 2.34 (s, 3H), 1.89 (s, 2H); MS (EI) for Ci6Hi7BrN4O2: 377:379 (Bromine isotope MH+).
(Compound 476) 9- { [(3 S)- 3 -hydroxypyrrolidin- 1 - yllmethyl|[l,31dioxolo[4,51[llbenzo-uro3,2-dlpyrimidin-7(8H)-one [00250] 9-{[(3S)-3-hydroxypyrrolidin-l- yl]methyl}[l,3]dioxolo[4,5][l]benzofuro[3,2-d]pyrimidin-7(8H)-one was synthesized in a manner similar to Example 1, wherein 5-bromo-2-hydroxybenzonitrile was replaced by 5-hydroxybenzo[d][l,3]dioxole-4-carbonitrile at the start of the sequence and piperidine was substituted for S-(l)-3-pyrrolidinol in the final step. Purification of the desired compound was accomplished by preparative reverse phase chromatography to afford the title compound (1H NMR (400 MHz, d6-DMSO): 7.24 (AB, 2H), 6.24 (s, 2H), 4.19 (m, IH), 3.64(m, 2H), 2.76 (m, 2H), 2.51 (m, 2H), 2.02 (m, IH), 1.58 (m, IH); MS (EI) for Ci6Hi5N3O5: 330.2 (MH+).
(Compound 5) 8-chloro-2-(pyrrolidin-l-ylmethyl)rilbenzofuror3,2-dlpyrimidin-4(3H)-one (HCl salt) [00251] 8-chloro-2-(pyrrolidin-l-ylmethyl)[l]benzofuro[3,2-d]pyrimidin-4(3H)- one was synthesized in a manner similar to Example 1 wherein 8-bromo-2- (chloromethyl)benzofuro[3,2-d]pyrimidin-4(3H)-one 6 was substituted with 8-chloro- 2-(chloromethyl)benzofuro[3,2-d]pyrimidin-4(3H)-one and piperidine was substituted with pyrrolidine. Purification by preparative HPLC (reverse-phase, acetonitrile/water by preparative HPLC (reverse-phase, acetonitrile/water with 10 rnM ammonium acetate) and (reverse-phase, 0.1% TFA in acetonitrile/0.05% TFA in water) was followed by concentration in vacuo and lyophilization afforded the title compound (19.8 mg, 11.7%).
1H NMR (400 MHz, d6-DMSO): 8.17 (d, IH), 7.94 (d, IH), 7.75 (d, IH), 4.56 (s, 2H), 3.48-3.26 (m, 4H, overlapped), 2.01 (m, 4H); MS (EI) for Ci5Hi4ClN3O2: 304 (MH+). (Compound 7)
8-chloro-2-[(4-methylpiperazin-l-yl)methyll[llbenzofuro[3,2-dlpyrimidin-4(3H)-one (HCl salt)
[00252] 8-chloro-2-[(4-methylpiperazin-l-yl)methyl][l]benzofuro[3,2- d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 1, Compound 2, wherein 8-bromo-2-(chloromethyl)benzofuro[3,2-d]pyrimidin-4(3H)- one 6 was substituted with 8-chloro-2-(chloromethyl)benzofuro[3,2-d]pyrimidin- 4(3H)-one and piperidine was substituted with N-methylpiperazine. Purification by preparative HPLC (reverse-phase, acetonitrile/water with 10 mM ammonium acetate) and (reverse-phase, 0.1% TFA in acetonitrile/0.05% TFA in water) was followed by concentration in vacuo and lyophilization afforded the title compound (12 mg, 6.5%). 1H NMR (400 MHz, d6-DMSO): 8.08 (d, IH), 7.91 (d, IH), 7.72 (dd, IH), 3.66 (s, 2H), 3.05 (m, 5H), 2.78 (s, 3H), 2.59 (m, 3H); MS (EI) for Ci6Hi7ClN4O2: 333 (MH+).
(Compound 11) 8-bromo-2- { r(2-chlorophenyl)aminolmethyl| \ 1 lbenzofuro [3 ,2-d1pyrimidin-4(3H)- one
[00253] 8-bromo-2-{[(2-chlorophenyl)amino]methyl}[l]benzofuro[3,2- d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 1, Compound 2, wherein piperidine was substituted with commercially available 2- Chloro-aniline. Precipitation from ethanol gave 47 mg (53% Yield) of 8-bromo-2-
{[(2-chlorophenyl)amino]methyl} [l]benzofuro[3,2-d]pyrimidin-4(3H)-one. 1H NMR (400MHz, d6-DMSO): 13.05 (br s, IH), 8.20 (s,lH), 7.82 (s, 2H), 7.31 (d, IH), 7.19 (t, IH), 6.75 (d, 1 H), 6.63 (t, IH), 6.05 (br t, IH), 4.41 (br d, 2H); MS (EI) for Ci7HnBrClN3O2: 404 : 406 (Bromine isotope MH+).
Compound 12)
8-bromo-2-{r(3-fluorophenyl)aminolmethvUrilbenzofuror3,2-dlpyrimidin-4(3H)-one [00254] 8-bromo-2-{[(3-fluorophenyl)amino]methyl} [l]benzofuro[3,2- d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 1, Compound 2, wherein piperidine was substituted with 3-fluoroaniline. Purification by preparative HPLC, followed by concentration in vacuo and lyophilization afforded the title compound (9.8 mg, 8%) as a white solid. 1H NMR (400 MHz, d6-DMSO): 8.17 (m, IH), 7.82 (m, 2H), 7.10 (dd, IH), 6.49 (m, IH), 6.35 (td, IH), 4.32 (d, 2H) ); MS (EI) for Ci7HnBrFN3O2: 388 (MH+).
(Compound 13)
8-bromo-2-[(pyridin-3-ylamino)methyll[llbenzofuro[3,2-dlpyrimidin-4(3H)-one (acetate salt) [00255] 8-bromo-2-[(pyridin-3-ylamino)methyl][l]benzofuro[3,2-d]pyrimidin-
4(3H)-one was synthesized in a manner similar to Example 1, Compound 2, wherein piperidine was substituted with 3-aminopyridine. Purification by preparative HPLC, followed by concentration in vacuo and lyophilization afforded the title compound (42 mg, 52%) as a white solid. 1H NMR (400 MHz, d6-DMSO): 8.19 (m, IH), 8.13 (m, IH), 7.71 (dd, IH), 7.63 (m, 2H), 7.59 (dd, IH), 6.62 (br s, IH), 5.50 (s, IH), 1.9 (s, IH); MS (EI) for Ci6HnBrN4O2: 371 (MH+).
(Compound 16)
8-bromo-2-r(phenylamino)methyliπibenzofuror3,2-dlpyrimidin-4(3H)-one
[00256] 8-bromo-2-[(phenylamino)methyl][l]benzofuro[3,2-d]pyrimidin-4(3H)- one was synthesized in a manner similar to Example 1, Compound 2, wherein piperidine was substituted with aniline. Purification by preparative HPLC, followed by concentration in vacuo and lyophilization afforded the title compound (12 mg, 15%) as a white solid. 1U NMR (400 MHz, d6-DMSO): 8.19 (m, IH), 7.82 (m, 2H), 7.11 (td, 2H), 6.67 (dd, 2H), 6.58 (t, IH), 6.14 (m, IH), 4.33 (d, 2H); MS (EI) for CnHi2BrN3O2: 370 (MH+).
(Compound 19)
8-bromo-2- { [(2-fluorophenyl)amino"|methyl| [ 1 lbenzofuro[3 ,2-dlpyrimidin-4(3H)-one [00257] 8-bromo-2-{[(2-fluorophenyl)amino]methyl}[l]benzofuro[3,2- d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 1, Compound 2, wherein piperidine was substituted with 2-fluoroaniline. Purification by preparative HPLC, followed by concentration in vacuo and lyophilization afforded the title compound (51 mg, 41%) as a white solid. 1H NMR (400 MHz, d6-DMSO):
8.17 (m, IH), 7.82 (m, 2H), 7.07 (m, IH), 6.94 (m, IH), 6.74 (m, IH), 6.62 (m, IH), 6.00 (m, IH), 4.39 (d, 2H) ; MS (EI) for Ci7HnBrFN3O2: 388 (MH+).
(Compound 21)
8-bromo-2-[( { r3-(dimethylamino)phenvHmethyl| amino)methvH [ 1 lbenzofuro[3 ,2- dlpyrimidin-4(3H)-one
[00258] 8-bromo-2-[({[3-(dimethylamino)phenyl]methyl}amino)methyl][l]- benzofuro[3,2-d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 1, Compound 2, wherein piperidine was substituted with commercially available N-[3-(Aminomethyl)phenyl]-N,N-dimethylamine. Preparative HPLC gave 9 mg (10% Yield) of 8-bromo-2-[({[3-
(dimethylamino)phenyl]methyl} amino)methyl] [ 1 ]benzofuro[3 ,2-d]pyrimidin-4(3H)- one. 1H NMR (400MHz, d6-DMSO): 9.81 (br s, 2H), 8.20 (s,lH), 7.85 (m, 2H), 7.31 (t, IH), 7.05 (m, IH), 6.91 (m, 2H), 4.29 (d, 2 H), 2.99 (s, 6H); MS (EI) for C20Hi9BrN4O2: 427:429 (Bromine isotope MH+). (Compound 22)
8-bromo-2- { r(4-fluorophenyl)aminolmethyl| \ 1 lbenzofuroB ,2-d1pyrimidin-4(3H)-one
[00259] 8-bromo-2-{[(4-fluorophenyl)amino]methyl}[l]benzofuro[3,2- d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 1, Compound 2, wherein piperidine was substituted with 4-fluoroaniline. Purification by preparative HPLC, followed by concentration in vacuo and lyophilization afforded the title compound (9.2 mg, 7.5%) as a white solid. 1U NMR (400 MHz, d6-DMSO):
8.18 (m, 2H), 7.82 (m, 2H), 6.93 (m, 2H), 6.65 (m, 2H), 6.1 (m, IH), 4.30 (d, 2H); MS (EI) for Ci7HnBrFN3O2: 388 (MH+).
(Compound 24) 8-bromo-2-(morpholin-4-ylmethyl)[llbenzofuro[3,2-dlpyrimidin-4(3H)-one
[00260] 8-bromo-2-(morpholin-4-ylmethyl)[l]benzofuro[3,2-d]pyrimidin-4(3H)- one was synthesized in a manner similar to Example 1, Compound 2, wherein piperidine was substituted with morpholine. Purification via recrystallization from hot ethanol afforded the title compound (41 mg, 37%) as a white solid. 1H NMR (400 MHz, de-DMSO): 12.73 (s, IH), 8.2 (m, IH), 7.81 (m, 2 H), 3.60 (m, 6H), 3.5 (s, 2H), 2.50 (m, 2H, overlapped); MS (EI) for Ci5Hi4BrN3O3: 364 (MH+). (Compound 74)
8-bromo-2-|Y { [2-(4-methylpiperazin- 1 -yl)phenyl]methyl| amino)methyl] [ 1 ] benzofuro[3,2-d]pyrimidin-4(3H)-one
[00261] 8-bromo-2-[( {[2-(4-methylpiperazin- 1 - yl)phenyl]methyl}amino)methyl][l]-benzofuro[3,2-d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 1, Compound 2, wherein piperidine was substituted with (2-(4-methylpiperazin-l-yl)phenyl)methanamine. The reaction mixture was concentrated in vacuo and purified by preparative HPLC (reverse-phase, 0.1% TFA in acetonitrile/0.05% TFA in water). Concentration in vacuo and lyophilization afforded the title compound (5.6 mg, 3.6%). 1H NMR (400 MHz, d6- DMSO): 8.17 (m, IH), 7.90 (m, 2H), 7.60 (m, IH), 7.48 (m, IH), 7.35 (m, IH), 7.30 (m, IH), 4.42 (m, 2H), 4.28 (m, 2H), 3.49 (m, 4H, overlapped), 3.01 (m, 4H), 2.82 (s, 3H). MS (EI) for C23H24BrN5O2: 482 (MH+).
(Compound 84)
8-bromo-2- ([4-(I -methylpiperidin-4-yDpiperazin- 1 -yl]methyl| [ 1 Ibenzofuro [3 ,2- dlpyrimidin-4(3H)-one
[00262] 8-bromo-2- { [4-( 1 -methylpiperidin-4-yl)piperazin- 1 - yl]methyl}[l]benzofuro[3,2-d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 1, Compound 2, wherein piperidine was substituted with 1-(1- Methylpiperidin-4-yl)piperazine. The product was precipitated from 1 : 1 DMSO:MeOH to afforded the title compound (90 mg, 61%) as a white solid. 1H
NMR (400 MHz, MeOD): 8.23 (d, IH), 7.76 (m, IH), 7.65 (d, IH), 3.65 (s, 2H), 2.98 (m, 2H), 2.69 (br s, 4H), 2.32 (m, 4H), 2.16 (t, 2H), 1.92 (m, 2H), 1.60 (q, 2H); MS (EI) for C2IH26BrN5O2: 460:462 (Bromine isoptope MH+).
(Compound 133) 8-bromo-2- { [(3 S)-3 -hydroxypyrrolidin- 1 -yl"|methyl| [ 1 Ibenzofuro [3 ,2-d]pyrimidin- 4(3HVone [00263] 8-bromo-2- { [(3 S)-3-hydroxypyrrolidin- 1 -yl]methyl} [ 1 ]benzofuro[3 ,2- d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 1, Compound 2, wherein piperidine was substituted with S-(l)-3-pyrrolidinol. Purification by flash chromatography afforded the title compound (69 mg, 55%). 1H NMR (400 MHz, d6-DMSO+ D2O): 8.22 (s, IH), 7.81 (s, 2H), 4.21 (m, IH), 3.76(m, 2H), 2.86 (m, 2H), 2.61 (m, 2H), 2.08 (m, IH), 1.65 (m, IH); MS (EI) for Ci5Hi4BrN3O3: 364:366 (Bromine isotope MH+).
(Compound 167)
2-(azetidin- 1 -ylmethvD-8-bromor 11benzofuro[3,2-d1pyrimidin-4(3H)-one [00264] 2-(azetidin- 1 -ylmethyl)-8-bromo[ 1 ]benzofuro[3,2-d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 1, Compound 2, wherein piperidine was substituted with azetidine. The reaction mixture was concentrated in vacuo and purified by preparative HPLC. Concentration in vacuo and lyophilization afforded the title compound (25 mg, 29%). 1H NMR (400 MHz, d6-DMSO): 8.18 (m, IH), 7.80 (m, 2H), 3.65 (s, 2H), 3.38 (t, 4H), 2.05 (q, 2H). MS (EI) for Ci4Hi2BrN3O2: 334 (MH+).
(Compound 191)
8-bromo-2- ([(U -dimethyl-2-piperidin- 1 -ylethyl)amino]methyl| [ 1 lbenzofuro[3 ,2- d"|pyrimidin-4(3H)-one [00265] 8-bromo-2- {[(1,1 -dimethyl-2-piperidin- 1 -ylethyl)amino]methyl} [I]- benzofuro[3,2-d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 1, Compound 2, wherein piperidine was substituted with l,l-Dimethyl-2- piperidin-l-ylethyl)amine. Purification by preparative HPLC, followed by concentration in vacuo and lyophilization afforded the title compound (13 mg, 5%). 1H NMR (400 MHz, d6-DMSO): 8.10 (d, IH), 7.77 (m, 2H), 3.84 (s, 2H), 2.33 (s, 2H), 1.58 (m, 4H), 1.38 (br s, 2H), 1.07 (br s,6H); MS (EI) for C20H25BrN4O2: 433:435 (Bromine isoptope MH+).
(Compound 212)
8-bromo-2-( {[1,1 -dimethyl-2-(4-methylpiperazin- 1 - yl)ethyl]amino|methyl)[l]benzofuro[3,2-d]pyrimidin-4(3H)-one
[00266] 8-bromo-2-( {[1,1 -dimethyl-2-(4-methylpiperazin- 1 - yl)ethyl]amino}methyl)[l]benzofuro[3,2-d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 1, Compound 2, wherein piperidine was substituted with 2-Methyl-l-(4-methylpiperazine-l-yl)propane-2-amine. Purification by preparative HPLC, followed by concentration in vacuo and lyophilization afforded the title compound (68 mg, 25%). 1H NMR (400 MHz, d6-DMSO): 8.09 (d, IH), 7.65 (m, 2H), 4.37 (s, 2H), 3.70 (br s, 2H), 3.42 (m, 2H), 3.14 (s, 3H), 1.67 (s,3H), 1.13 (s, 6H); MS (EI) for C20H26BrN5O2: 448:450 (Bromine isoptope MH+).
(Compound 247)
8-bromo-2- { [4-(2-hydroxyethyl)piperazin- 1 -yl]methyl| [ 1 Ibenzofuro [3 ,2-d]pyrimidin-
4(3HVone [00267] 8-bromo-2- { [4-(2-hydroxyethyl)piperazin- 1 -yl]methyl} [ 1 ]benzofuro[3 ,2- d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 1, Compound 2, wherein piperidine was substituted with N-(2-
Hydroxyethyl)piperazine. Precipitation from EtOH, followed by rinsing with MeOH afforded the title compound (38 mg, 30%). 1H NMR (400 MHz, d6-DMSO): 8.21 (m, IH), 7.83 (m, 2H), 3.5 l(s, 2H), 3.47 (t, 2H), 2.39 (m, 2H), 2.38 (t, 2H); MS (EI) for Ci7Hi9BrN4O3: 407:409 (Bromine isoptope MH+).
(Compound 51)
8-bromo-2-(2-hydroxyphenyl)[llbenzofuro[3,2-dlpyrimidin-4(3H)-one [00268] The title compound was synthesized in a manner similar to Example 1. A solution of 3-amino-5-bromobenzofuran-2-carboxamide 3 (0.5Og , 0.196 mmol) and 2-hydroxybenzaldehyde (3.92 mmol) in 6 mL anhydrous ethanol were combined and stirred at room temperature for 10 minutes. The resulting suspension was treated with concentrated hydrochloric acid (40 μL) and a precipitate formed immediately. The resulting slurry was diluted with additional anhydrous ethanol (10 ml) and the resulting slurry was heated at 80 0C for 16 hours. The resulting precipitate was filtered off and washed with ethyl acetate (2 x 50 ml) and methanol (2 x 5 ml) to give 8- bromo-2-(2-hydroxyphenyl)[l]benzofuro[3,2-d]pyrimidin-4(3H)-one. 1H NMR (400 MHz, de-DMSO): 8.38 (s, IH), 8.17 (dd, IH), 7.88 (m, 2H), 7.42 (t, IH), 7.0 (m, IH); MS (EI) for Ci6H9BrIN2O3: 357 (MH+). (Compound 204)
2- { [(3 S)-3 -hydroxypyrrolidin- 1 -yllmethyll \ 1 Ibenzofuro [3 ,2-d1pyrimidin-4(3H)-one [00269] 2- { [(3 S)-3 -hydroxypyrrolidin- 1 -yl]methyl} [ 1 ]benzofuro [3 ,2-d]pyrimidin- 4(3H)-one was synthesized in a manner similar to Example 1, Compound 2, wherein 8-bromo-2-(chloromethyl)benzofuro[3,2-d]pyrimidin-4(3H)-one 6 was substituted with 2-(chloromethyl)benzofuro[3,2-d]pyrimidin-4(3H)-one and piperidine was substituted with S-(-)-3-hydroxypyrrolidine. Purification by preparative HPLC resulting in 130 mg (90% Yield) of 2-{[(3S)-3-hydroxypyrrolidin-l- yl]methyl}[l]benzofuro[3,2-d]pyrimidin-4(3H)-one. 1H NMR (400 MHz, d6-DMSO): 8.24 (s, IH), 8.08 (d, IH), 7.75-7.63 (m, 2H), 7.45 (dd, IH), 4.53-4.50 (m, IH), 4.21 (d, IH), 4.19 (d, IH), 3.40-3.30 (m, IH), 3.20-3.05 (m, 3H), 2.32-2.22 (m, IH), 2.0- 1.95 (m, IH); MS (EI) for Ci5Hi5N3O3: 286 (MH+).
(Compound 311)
8-bromo-2-(3,9-diazaspiro[5.51undec-3-ylmethyl)[llbenzofuro[3,2-dlpyrimidin- 4(3HVone [00270] The compound was synthesized in a manner similar to Example 1 wherein a solution of 8-bromo-2-(chloromethyl)[l]benzofuro[3,2-d]pyrimidin-4(3H)-one 6 (100 mg, 0.319 mmol) in 5 mL anhydrous ethanol was added 3,9-diaza- spiro[5,5]undecane-3-carboxylic acid tert-butyl ester (254 mg, 1 eq). The reaction mixture was heated to 80 0C for 16 hours, cooled down. 4 N HCl in dioxane (2 mL) was added. The reaction mixture was heated to 80 0C for 1 hour. The reaction was cooled and concentrated. The purification of the residue by preparative HPLC gave 8- bromo-2-(3,9-diazaspiro[5.5]undec-3-ylmethyl)[l]benzofuro[3,2-d]pyrimidin-4(3H)- one (90 mg) as a white solid. 1H NMR (400 MHz, d6-DMSO): 13.55 (s, IH), 10.78 (s, IH), 9.0 (s, 2H), 8.21 (m, IH), 7.90 (m, 2H), 4.53 (s, 2H), 3.60-3.40 (m, 4H), 3.02 (s, 4H), 1.95-1.75 (m, 8H); MS (EI) for C20H23BrN4O2: 431 (MH+). (Compound 331)
8-bromo-2-(3-pyrrolin-l-ylmethyl)πibenzofuror3,2-dlpyrimidin-4(3H)-one
[00271] 8-bromo-2-(3-pyrrolin- 1 -ylmethyl)[ 1 ]benzofuro[3,2-d]pyrimidin-4(3H)- one was synthesized in a manner similar to Example 1, Compound 2, wherein piperidine was substituted with 3-pyrroline. Purification by preparative HPLC (reverse-phase, acetonitrile/water with 0.01% ammonium acetate), followed by concentration in vacuo and lyophilization afforded the title compound as a white solid. 1H NMR (400 MHz, d6-DMSO): 8.20 (s, IH), 7.82 (m, 2H), 5.83 (s, 1 H), 3.93
(s, 2H), 3.67 (s, 4H); MS (EI) for Ci5Hi2BrN3O2: 346 (MH+). (Compound 332)
[00272] 8-bromo-2-((7-hydroxy-2-azabicyclo[2.2.1 ]heptan-2- yl)methyl)benzofuro[3,2-d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 1, Compound 2, wherein piperidine was substituted with anti-7-hydroxy- 2-azabicyclo[2.2. ljheptane. Purification by preparative HPLC (reverse-phase, acetonitrile/water with 0.01% ammonium acetate), followed by concentration in vacuo and lyophilization afforded the title compound as a racemer. 1H NMR (400 MHz, de-DMSO): 8.19 (s, IH), 7.82 (m, 2H), 4.12 (s, 1 H), 3.78 (d, IH), 3.65 (d, IH), 3.02 (m, 2H), 2.27 (d, IH), 2.0 (m, IH), 1.80 (m, 2H), 1.67 (m, IH), 1.27 (m, IH); MS (EI) for Ci7Hi6BrN3O3: 390 (MH+).
(Compound 333)
8-bromo-2-(((3S.4S)-3.4-dihvdroxypyrrolidin-l-vnmethvnbenzofuror3.2- d"|pyrimidin-4(3H)-one [00273] To a solution of 8-bromo-2-(chloromethyl)[l]benzofuro[3,2-d]pyrimidin- 4(3H)-one 6 (Example 1) (142 mg, 0.45 mmol) in 5 mL anhydrous ethanol was added (3S, 4S)-3,4-bis[(tert-butyl-dimethylsilyl)oxy]pyrrolidine (150 mg, 0.45 mmol). The reaction mixture was heated to 80 0C for 16 hours. 2 N HCl aq. (2 mL) was added. The reaction mixture was further heated at 80 0C for 1 hour. The reaction was cooled and concentrated. The purification of the residue by preparative HPLC gave the title compound as a white solid. 1H NMR (400 MHz, d6-DMSO): 8.21 (s, IH), 8.18 (s, IH), 7.82 (m, 2H), 3.87 (m, 2H), 3.75 (dd, 2H), 2.99 (m, 2H), 2.45 (m, 2H); MS (EI) for Ci5Hi4BrN3O4: 380 (MH+).
(Compound 344)
8-bromo-2- { r(2R)-2-(hvdroxymethyl)pyrrolidin- 1 -yllmethyll \ 1 lbenzofuro[3 ,2- dlpyrimidin-4(3H)-one
[00274] 8-bromo-2- { [(2R)-2-(hydroxymethyl)pyrrolidin- 1 - yl]methyl}[l]benzofuro[3,2-d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 1, Compound 2, wherein piperidine was substituted with D- prolinol. Purification by preparative HPLC (reverse-phase, acetonitrile/water with 0.01% ammonium acetate), followed by concentration in vacuo and lyophilization afforded the title compound as a white solid. 1H NMR (400 MHz, d6-DMSO): 8.19 (s, IH), 7.82 (m, 2H), 4.02 (d, 1 H), 3.57 (d, IH), 3.48 (m, 2H), 2.95 (m, 2H), 2.70 (m, IH), 2.37 (m, IH), 1.92 (m, IH), 1.67 (m, IH), 1.27 (m, IH); MS (EI) for Ci6Hi6BrN3O3: 378 (MH+).
(Compound 348)
8-bromo-2- { [ cis-3 ,4-dihvdroxypyrrolidin- 1 -yllmethyl) \ 1 ibenzofuroB ,2-dlpyrimidin- 4(3HVone
[00275] 8-bromo-2- { [cis-3 ,4-dihydroxypyrrolidin- 1 -yl]methyl} [ 1 ]benzofuro[3 ,2- d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 1, Compound 2, wherein piperidine was substituted with cis-3, 4-dihydroxypyrrolidine (prepared above). Purification by preparative HPLC (reverse-phase, acetonitrile/water with 0.01% ammonium acetate), followed by concentration in vacuo and lyophilization afforded the title compound as a white solid. 1H NMR (400 MHz, d4- CDOD3): 8.58 (s, IH), 7.81 (d, IH), 7.72 (d, IH), 4.65 (s, 2H), 4.42 (m, 2H), 3.75 (m, 2H), 3.65 (m, 2H); MS (EI) for Ci5Hi4BrN3O4: 380 (MH+).
(Compound 364) [00276] N-((3R)-l-r(8-bromo-4-oxo-3.4-dihvdrorilbenzofuror3.2-dlpyrimidin-2- yl)methyllpyrrolidin-3-vUacetamide
N-{(3R)-l-[(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2- yl)methyl]pyrrolidin-3-yl}acetamide was synthesized in a manner similar to Example 1, Compound 2, wherein piperidine was substituted with (3R)-(+)-3- acetamidopyrrolidine. Purification by preparative HPLC (reverse-phase, acetonitrile/water with 0.01% ammonium acetate), followed by concentration in vacuo and lyophilization afforded the title compound as a white solid. 1H NMR (400 MHz, d6-DMSO): 7.40 (m, IH), 6.98 (m, IH), 6.85 (d, IH), 3.60 (m, IH), 3.07 (s, 2H), 2.20 (m, 2H), 1.95 (m, 2H), 1.50 (m, IH), 1.09 (s, 3H), 0.99 (m, IH); MS (EI) for Ci7Hi7BrN4O3: 405 (MH+).
(Compound 365)
N-{(3S)-l-[(8-bromo-4-oxo-3,4-dihydro[llbenzofuro[3,2-dlpyrimidin-2- yl)methyl]pyrrolidin-3-yUacetamide
[00277] N-{(3S)-l-[(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2- yl)methyl]pyrrolidin-3-yl}acetamide was synthesized in a manner similar to Example 1, Compound 2, wherein piperidine was substituted with (3S)-(-)-3- acetamidopyrrolidine. Purification by preparative HPLC (reverse-phase, acetonitrile/water with 0.01% ammonium acetate), followed by concentration in vacuo and lyophilization afforded the title compound as a white solid. 1H NMR (400 MHz, d6-DMSO): 8.22 (s, IH), 8.10 (m, IH), 7.81 (m, 2H), 4.20 (m, IH), 3.70 (dd, 2H), 2.80 (m, 2H), 2.40 (m, 2H), 2.10 (m, IH), 1.80 (s, 3H), 1.58 (m, IH); MS (EI) for Ci7Hi7BrN4O3: 405 (MH+).
(Compound 369)
8-bromo-2- { [(3 S)- 3 -(methyloxy)pyrrolidin- 1 -yl"|methyl| [ 1 Ibenzofuro [3 ,2- d"|pyrimidin-4(3H)-one
[00278] 8-bromo-2- { [(3 S)-3 -(methyloxy)pyrrolidin- 1 -yl]methyl} [ 1 ]benzofuro [3 ,2- d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 1,
Compound 2, wherein piperidine was substituted with (S)-3-methoxypyrrolidine. Purification by preparative HPLC (reverse-phase, acetonitrile/water with 0.01% ammonium acetate), followed by concentration in vacuo and lyophilization afforded the title compound as a white solid. 1H NMR (400 MHz, d6-DMSO): 8.22 (s, IH), 7.81 (m, 2H), 3.93 (m, IH), 3.65 (s, 2H), 3.17 (s, 3H), 2.82 (dd, IH), 2.70 (m, IH), 2.58 (m, 2H), 2.0 (m, IH), 1.68 (m, IH); MS (EI) for Ci6Hi6BrN3O3: 378 (MH+).
(Compound 408)
8-bromo-2- { [(3R)-3-(methyloxy)pyrrolidin- 1 -yl"|methyl| [ 1 "Ibenzofuro [3 ,2- d"|pyrimidin-4(3H)-one [00279] 8-bromo-2-{[(3R)-3-(methyloxy)pyrrolidin-l-yl]methyl} [l]benzofuro[3,2- d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 1, Compound 2, wherein piperidine was substituted with (3K)-(-)-N-tert- butoxycarbonyl-3-hydroxypyrrolidine. 1U NMR (400 MHz, d6-DMSO): 8.22 (s, IH), 7.81 (m, 2H), 3.93 (m, IH), 3.65 (s, 2H), 3.17 (s, 3H), 2.82-2.65 (m, 2H), 2.58 (m, 2H), 1.95 (m, IH), 1.68 (m, IH); MS (EI) for Ci6Hi6BrN3O3: 378 (MH+).
(Compound 370) l-r(8-bromo-4-oxo-3,4-dihvdrorilbenzofuror3,2-dlpyrimidin-2-yl)methyllazetidine- 3-carboxylic acid
[00280] l-[(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2- yl)methyl]azetidine-3-carboxylic acid was synthesized in a manner similar to Example 1, Compound 2, wherein piperidine was substituted with azetidine-3- carboxylic acid. Purification by preparative HPLC (reverse-phase, acetonitrile/water with 0.01% ammonium acetate), followed by concentration in vacuo and lyophilization afforded the title compound as a white solid. 1H NMR (400 MHz, d6- DMSO): 8.29 (s, IH), 8.22 (s, IH), 7.82 (m, 2H), 3.67-3.55 (m, 4H), 3.40 (t, 2H), 3.22 (m, IH); MS (EI) for Ci5Hi2BrN3O4: 378 (MH+). (Compound 398)
8-bromo-2-[(3-hydroxy-8-azabicyclo[3.2.1 "|oct-8-yl)methyll [ 1 "|benzofuro|"3,2- d"|pyrimidin-4(3H)-one
[00281] 8-bromo-2-[(3-hydroxy-8-azabicyclo[3.2. l]oct-8- yl)methyl][l]benzofuro[3,2-d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 1, Compound 2, wherein piperidine was substituted with 8-aza- bicyclo[3,2,l]octan-3-ol. Purification by preparative HPLC (reverse-phase, acetonitrile/water with 0.01% ammonium acetate), followed by concentration in vacuo and lyophilization afforded the title compound as a white solid. 1H NMR (400 MHz, d6-DMSO): 8.22 (m, IH), 7.77 (m, 2H), 3.77-3.35 (m, 6H), 2.20-1.90 (m, 5H), 1.80-179 (m, 2H); MS (EI) for Ci8Hi8BrN3O3: 404 (MH+).
(Compound 366)
8-bromo-2- ( IY2Sy2-(hvdroxymethv0pyrrolidin- 1 -yllmethvU \ 1 ibenzofuroB .2- d"|pyrimidin-4(3H)-one
[00282] 8-bromo-2- { [(2S)-2-(hydroxymethyl)pyrrolidin- 1 - yljmethyl} [l]benzofuro[3,2-d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 1, Compound 2, wherein piperidine was substituted with L- prolinol. Purification by preparative HPLC (reverse-phase, acetonitrile/water with 0.01% ammonium acetate), followed by concentration in vacuo and lyophilization afforded the title compound as a white solid. 1H NMR (400 MHz, d6-DMSO): 8.21 (s, IH), 7.82 (m, 2H), 4.02 (d, 1 H), 3.57 (d, IH), 3.47 (dd, IH), 3.37 (dd, IH), 2.95 (m, 2H), 2.70 (m, IH), 2.37 (m, IH), 1.82 (m, IH), 1.69 (m, 3H); MS (EI) for Ci6Hi6BrN3O3: 378 (MH+).
(Compound 199)
8-bromo-2-[(cyclopentylamino)methyll[llbenzofuro[3,2-dlpyrimidin-4(3H)-one [00283] 8-bromo-2-[(cyclopentylamino)methyl] [ 1 ]benzofuro[3,2-d]pyrimidin-
4(3H)-one was synthesized in a manner similar to Example 1, Compound 2, wherein piperidine was substituted with cyclopentylamine. Purification by preparative HPLC (reverse-phase, acetonitrile/water with 0.1% formic acid), followed by concentration in vacuo and lyophilization afforded the title compound (11.2 mg). 1H NMR (400 MHz, de-DMSO): 8.51 (s, IH), 8.14-8.16 (m, IH), 7.76-7.79 (m, 2H), 3.79 (s, 2H), 3.12-3.19 (m, IH), 1.74-1.83 (m, 2H), 1.60-1.64 (m, 2H), 1.36-1.53 (m, 4H). MS (EI) for Ci6Hi6BrN3O2: 362 (MH+).
(Compound 200)
8-bromo-2-({[2-(dimethylamino)ethyllamino|methyl)[llbenzofuro[3,2-dlpyrimidin- 4(3H)-one
[00284] 8-bromo-2-({[2-(dimethylamino)ethyl]amino}methyl)[l]benzofuro[3,2- d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 1, Compound 2, wherein piperidine was substituted with N,N-dimethyl- ethylenediamine. Purification by preparative HPLC (reverse-phase, acetonitrile/water with 0.1% formic acid), followed by concentration in vacuo and lyophilization afforded the title compound (7.3 mg). 1H NMR (400 MHz, d6-DMSO): 8.23 (s, IH), 8.00-8.03 (m, IH), 7.64-7.70 (m, IH), 4.37 (s, 2H), 3.41-3.50 (m, 2H), 3.23 (s, 6H), 3.04-3.10 (m, 2H). MS (EI) for Ci5Hi7BrN4O2: 365 (MH+).
(Compound 201)
8-bromo-2-|Y4-methylpiperidin- 1 -yDmethyl] [ 1 ]benzofuro[3 ,2-dlpyrimidin-4(3H)-one
[00285] 8-bromo-2-[(4-methylpiperidin- 1 -yl)methyl] [ 1 ]benzofuro[3 ,2- d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 1, Compound 2, wherein piperidine was substituted with 4-methylpiperidine. Purification by preparative HPLC (reverse-phase, acetonitrile/water with 0.1% formic acid), followed by concentration in vacuo and lyophilization afforded the title compound (26 mg). 1H NMR (400 MHz, d6-DMSO): 8.21 (s, IH), 7.82 (s, 2H), 3.50 (s, 2H), 2.81-2.92 (m, 2H), 2.06-2.17 (m, 2H), 1.52-1.62 (m, 2H), 1.33 (s, IH), 1.11- 1.26 (m, 2H), 0.89 (d, 3H). MS (EI) for CnHi8BrN3O2: 376 (MH+).
(Compound 202)
2-(l ,4'-bipiperidin- 1 '-ylmethyl)-8-bromo[ 1 lbenzofuro|"3,2-d"|pyrimidin-4(3H)-one
[00286] 2-(l ,4'-bipiperidin- 1 '-ylmethyl)-8-bromo[ 1 ]benzofuro[3,2-d]pyrimidin- 4(3H)-one was synthesized in a manner similar to Example 1, Compound 2, wherein piperidine was substituted with 4-piperidinopiperidine. Purification by preparative HPLC (reverse-phase, acetonitrile/water with 0.1% formic acid), followed by concentration in vacuo and lyophilization afforded the title compound (27 mg). 1H NMR (400 MHz, d6-DMSO): 8.20-8.23 (m, IH), 7.80-7.85 (m, 2H), 3.51 (s, 4H), 2.93 (d, 2H), 2.23-2.35 (m, IH), 2.07-2.18 (m, 2H), 1.68 (d, 2H), 1.45-1.58 (m, 6H), 1.34-1.42 (m, 2H). MS (EI) for C2IH25BrN4O2: 445 (MH+). (Compound 232)
8-bromo-2- { [( 1 -methylpropyl)amino"|methyl| [ 1 lbenzofuro[3 ,2-d]pyrimidin-4(3H)- one
[00287] 8-bromo-2- { [( 1 -methylpropyl)amino]methyl} [ 1 ]benzofuro[3 ,2- d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 1, Compound 2, wherein piperidine was substituted with sec-butylamine. Purification by preparative HPLC (reverse-phase, acetonitrile/water with 0.1% formic acid), followed by concentration in vacuo and lyophilization afforded the title compound (19.2 mg). 1H NMR (400 MHz, d6-DMSO): 8.24 (s, IH), 8.16 (s, IH), 7.80 (m, IH), 3.78-3.87 (m, 2H), 2.61-2.74 (m, IH), 1.47-1.60 (m, IH), 1.27-1.41 (m, 2H), 1.02- 1.10 (m, 3H), 0.84-0.90 (m, 3H). MS (EI) for Ci5Hi6BrN3O2: 350 (MH+).
(Compound 235)
8-bromo-2-( { [(2-chlorophenyl)methyl"|amino I methyl) [ 1 Ibenzofuro [3 ,2-d]pyrimidin- 4(3H)-one
[00288] 8-bromo-2-({[(2-chlorophenyl)methyl]amino}methyl)[l]benzofuro[3,2- d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 1, Compound 2, wherein piperidine was substituted with 2-chlorobenzylamine. Purification by preparative HPLC (reverse-phase, acetonitrile/water with 0.1% formic acid), followed by concentration in vacuo and lyophilization afforded the title compound (17.8 mg). 1H NMR (400 MHz, d6-DMSO): 8.18-8.22 (m, IH), 7.80-7.86 (m, 2H), 7.54-7.59 (m, IH), 7.38-7.42 (m, IH), 7.22-7.35 (m, 2H), 3.86 (s, 2H), 3.80 (s, 2H). MS (EI) for Ci8Hi3BrClN3O2: 418 (MH+).
(Compound 238)
8-bromo-2-|Y4-phenylpiperidin- 1 -yPmethyl] [ 1 ]benzofuro|"3 ,2-d"|pyrimidin-4(3H)-one
[00289] 8-bromo-2-[(4-phenylpiperidin- 1 -yl)methyl] [ 1 ]benzofuro[3 ,2-d]pyrimidin- 4(3H)-one was synthesized in a manner similar to Example 1, Compound , wherein piperidine was substituted with 4-phenylpiperidine. Purification by preparative HPLC (reverse-phase, acetonitrile/water with 0.1% formic acid), followed by concentration in vacuo and lyophilization afforded the title compound (33.8 mg). 1H NMR (400 MHz, de-DMSO): 11.35 (s, IH), 8.65 (s, IH), 8.20-8.24 (m, IH), 7.80-7.86 (m, 2H), 7.14-7.36 (m, 4H), 3.59 (s, 2H), 2.97-3.06 (m, 2H), 2.22-2.36 (m, 2H), 1.90-1.97 (m, IH), 1.65-1.83 (m, 4H). MS (EI) for C22H20BrN3O2: 438 (MH+). (Compound 240)
8-bromo-2- |Y4-pyridin-3 -ylpiperazin- 1 -yPmethyl] [ 1 ]benzofuro [3 ,2-d]pyrimidin- 4(3H)-one
[00290] 8-bromo-2-[(4-pyridin-3-ylpiperazin- 1 -yl)methyl] [ 1 ]benzofuro[3 ,2- d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 1, Compound 2, wherein piperidine was substituted with l-pyridin-3-yl piperazine.
Purification by preparative HPLC (reverse-phase, acetonitrile/water with 0.1% formic acid), followed by concentration in vacuo and lyophilization afforded the title compound (15.4 mg). 1H NMR (400 MHz, d6-DMSO): 8.30 (s, IH), 8.21 (s, IH), 7.96-8.01 (m, IH), 7.79-7.85 (m, 2H), 7.28-7.34 (m, IH), 7.18-7.24 (m, IH), 3.60 (s, 2H), 3.22 (s, 4H), 2.69 (s, 4H). MS (EI) for C20Hi8BrN5O2: 440 (MH+).
(Compound 241)
8-bromo-2-|Y2,3-dihydro- 1 H-inden- 1 -ylamino)methyl] [ 1 ]benzofuro[3 ,2-d]pyrimidin- 4(3H)-one
[00291] 8-bromo-2-[(2,3-dihydro-lH-inden-l-ylamino)methyl][l]benzofuro[3,2- d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 1,
Compound 2, wherein piperidine was substituted with 1-aminoindan. Purification by preparative HPLC (reverse-phase, acetonitrile/water with 0.1% formic acid), followed by concentration in vacuo and lyophilization afforded the title compound (20.0 mg). 1H NMR (400 MHz, d6-DMSO): 8.21-8.24 (m, IH), 7.79-7.85 (m, 2H), 7.41-7.46 (m, IH), 7.17-7.26 (m, 3H), 4.20-4.26 (m, IH), 3.84 (s, 2H), 2.89-2.99 (m, IH), 2.64-2.80 (m, IH), 2.21-2.35 (m, IH), 1.74-1.87 (m, IH). MS (EI) for C20Hi6BrN3O2: 410 (MH+).
(Compound 242)
8-bromo-2-|Y4-hydroxypiperidin- 1 -yPmethyl] [ 1 ]benzofuro|"3 ,2-d"|pyrimidin-4(3H)- one
[00292] 8-bromo-2-[(4-hydroxypiperidin- 1 -yl)methyl] [ 1 ]benzofuro[3 ,2- d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 1, Compound 2, wherein piperidine was substituted with 4-hydroxy piperidine. Purification by preparative HPLC (reverse-phase, acetonitrile/water with 0.1% formic acid), followed by concentration in vacuo and lyophilization afforded the title compound (13.7 mg). 1H NMR (400 MHz, d6-DMSO): 8.20-8.26 (m, IH), 7.79-7.85 (m, 2H), 3.50 (s, 2H), 2.71-2.81 (m, IH), 2.17-2.27 (m, 2H), 1.67-1.76 (m, 2H), 1.35- 1.49 (m, 2H), 0.96-1.02 (m, 2H). MS (EI) for Ci6Hi6BrN3O3: 378 (MH+).
(Compound 252) 8-bromo-2-[(cyclobutylamino)methyll[llbenzofuro[3,2-dlpyrimidin-4(3H)-one
[00293] 8-bromo-2-[(cyclobutylamino)methyl][l]benzofuro[3,2-d]pyrimidin- 4(3H)-one was synthesized in a manner similar to Example 1, Compound 2, wherein piperidine was substituted with cyclobutylamine. Purification by preparative HPLC (reverse-phase, acetonitrile/water with 0.1% formic acid), followed by concentration in vacuo and lyophilization afforded the title compound (9.3 mg). 1H NMR (400 MHz, de-DMSO): 8.31 (s, IH), 8.17-8.19 (m, IH), 7.78-7.83 (m, 2H), 3.69 (s, 2H), 3.23-3.32 (m, IH), 1.99-2.10 (m, 2H), 1.67-1.79 (m, 2H), 1.46-1.66 (m, 2H). MS (EI) for Ci5Hi4BrN3O2: 348 (MH+).
(Compound 253)
8-bromo-2- { [4-(phenylmethyl)piperidin- 1 -yl]methyl| [ 1 lbenzofuro[3 ,2-d]pyrimidin- 4(3HVone [00294] 8-bromo-2-{[4-(phenylmethyl)piperidin-l-yl]methyl} [l]benzofuro[3,2- d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 1, Compound 2, wherein piperidine was substituted with 4-benzyl piperidine. Purification by preparative HPLC (reverse-phase, acetonitrile/water with 0.1% formic acid), followed by concentration in vacuo and lyophilization afforded the title compound (22.1 mg). 1H NMR (400 MHz, d6-DMSO): 8.31 (s, IH), 8.19-8.21 (m, IH), 7.78-7.85 (m, 2H), 7.23-7.31 (m, 2H), 7.11-7.20 (m, 3H), 3.49 (s, 2H), 2.86 (d, 2H), 2.06 (t, 2H), 1.43-1.58 (m, 3H), 1.18-1.30 (m, 2H). MS (EI) for C23H22BrN3O2: 452 (MH+).
(Compound 254) 8-bromo-2- { [(pyridin-2-ylmethyl)amino"|methyl| [ 1 lbenzofuro[3 ,2-d]pyrimidin- 4(3H)-one
[00295] 8-bromo-2- { [(pyridin-2-ylmethyl)amino]methyl} [ 1 ]benzofuro[3 ,2- d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 1, Compound 2, wherein piperidine was substituted with 2-picolylamine. Purification by preparative HPLC (reverse-phase, acetonitrile/water with 0.1% formic acid), followed by concentration in vacuo and lyophilization afforded the title compound (22.4 mg). 1U NMR (400 MHz, d6-DMSO): 8.49-8.52 (m, IH), 8.18-8.21 (m, IH), 7.80-7.85 (m, 2H), 7.71-7.78 (m, IH), 7.45 (d, IH), 7.22-7.27 (m, IH), 3.90 (s, 2H), 3.83 (s, 2H). MS (EI) for Ci7Hi3BrN4O2: 385 (MH+).
(Compound 255)
8-bromo-2-|Y { [3-(methyloxy)phenyl]methyl| amino)methyl] [ 1 "|benzofuro|"3 ,2- dlpyrimidin-4(3H)-one [00296] 8-bromo-2-[({[3-
(methyloxy)phenyl]methyl}amino)methyl][l]benzofuro[3,2-d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 1, Compound 2, wherein piperidine was substituted with 3-methoxybenzylamine. Purification by preparative HPLC (reverse-phase, acetonitrile/water with 0.1% formic acid), followed by concentration in vacuo and lyophilization afforded the title compound (20.7 mg). 1H NMR (400 MHz, de-DMSO): 8.22 (s, IH), 8.18-8.21 (m, IH), 7.80-7.85 (m, 2H), 7.19-7.25 (m, IH), 6.90-6.96 (m, 2H), 6.76-6.84 (m, 2H), 3.76 (s, 2H), 3.74 (s, 2H), 3.72 (s, 3H). MS (EI) for Ci9Hi6BrN3O3: 414 (MH+).
(Compound 256) 8-bromo-2-( { [(3 -chlorophenyl)methyl"|amino I methyl) [ 1 Ibenzofuro [3 ,2-d]pyrimidin- 4(3H)-one
[00297] 8-bromo-2-({[(3-chlorophenyl)methyl]amino}methyl)[l]benzofuro[3,2- d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 1, Compound 2, wherein piperidine was substituted with 3-chlorobenzylamine. Purification by preparative HPLC (reverse-phase, acetonitrile/water with 0.1% formic acid), followed by concentration in vacuo and lyophilization afforded the title compound (25.3 mg). 1H NMR (400 MHz, d6-DMSO): 8.18-8.21 (m, 2H), 7.80-7.85 (m, 2H), 7.46 (s, IH), 7.25-7.35 (m, 3H), 3.78 (s, 2H), 3.75 (s, 2H). MS (EI) for Ci8Hi3BrClN3O2: 418 (MH+). (Compound 257)
8-bromo-2-[(4-morpholin-4-ylpiperidin- 1 -yDmethyl] [ 1 "|benzofuro|"3 ,2-d]pyrimidin- 4(3HVone [00298] 8-bromo-2-[(4-morpholin-4-ylpiperidin- 1 -yl)methyl] [ 1 ]benzofuro[3 ,2- d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 1, Compound 2, wherein piperidine was substituted with 4-morpholino piperidine. Purification by preparative HPLC (reverse-phase, acetonitrile/water with 0.1% formic acid), followed by concentration in vacuo and lyophilization afforded the title compound (16.3 mg). 1H NMR (400 MHz, d6-DMSO): 8.26 (s, IH), 8.18-8.21 (m, IH), 7.80-7.85 (m, 2H), 3.53-3.59 (m, 7H), 2.92 (d, 2H), 2.39-2.48 (m, 3H), 2.06-2.19 (m, 3H), 1.74 (d, 2H), 1.37-1.49 (m, 2H). MS (EI) for C20H23BrN4O3: 447 (MH+).
(Compound 258) 8-bromo-2- { r(furan-2-ylmethyl)aminolmethvU \ 1 lbenzofuro[3 ,2-d1pyrimidin-4(3H)- one
[00299] 8-bromo-2- { [(furan-2-ylmethyl)amino]methyl} [ 1 ]benzofuro[3 ,2- d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 1, Compound 2, wherein piperidine was substituted with furfurylamine. Purification by preparative HPLC (reverse-phase, acetonitrile/water with 0.1% formic acid), followed by concentration in vacuo and lyophilization afforded the title compound (9.7 mg). 1H NMR (400 MHz, d6-DMSO): 8.35 (s, IH), 8.18-8.21 (m, IH), 7.80-7.83 (m, 2H), 7.53-7.57 (m, IH), 6.34-6.37 (m, IH), 6.25-6.28 (m, IH), 3.76 (s, 2H), 3.74 (s, 2H). MS (EI) for Ci6Hi2BrN3O3: 374 (MH+). (Compound 259)
8-bromo-2-({[2-(lH-imidazol-4-yl)ethyllamino|methyl)[llbenzofuro[3,2- dlpyrimidin-4(3H)-one
[00300] 8-bromo-2-({[2-(lH-imidazol-4-yl)ethyl]amino}methyl)[l]benzofuro[3,2- d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 1, Compound 2, wherein piperidine was substituted with histamine. Purification by preparative HPLC (reverse-phase, acetonitrile/water with 0.1% formic acid), followed by concentration in vacuo and lyophilization afforded the title compound (3.0 mg). 1H NMR (400 MHz, d6-DMSO): 8.20 (s, IH), 8.15-8.17 (m, IH), 7.78-7.83 (m, 2H), 7.54 (d, IH), 6.81 (s, IH), 3.84 (s, 2H), 2.86 (t, 2H), 2.66-2.73 (m, 2H). MS (EI) for Ci6Hi4BrN5O2: 388 (MH+).
(Compound 260)
8-bromo-2-r(4-phenylprperazin- 1 -yl)methyll [ 1 "IbenzofuroB ,2-d1pyrimidin-4(3H)-one [00301] 8-bromo-2-[(4-phenylpiperazin-l-yl)methyl][l]benzofuro[3,2- d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 1, Compound 2, wherein piperidine was substituted with 1 -phenyl piperazine. Purification by preparative HPLC (reverse-phase, acetonitrile/water with 0.1% formic acid), followed by concentration in vacuo and lyophilization afforded the title compound (46.2 mg). 1H NMR (400 MHz, d6-DMSO): 8.21-8.23 (m, IH), 7.80-7.85 (m, 2H), 7.20 (t, 2H), 6.93 (d, 2H), 6.77 (t, IH), 3.60 (s, 2H), 3.13-3.18 (m, 4H), 2.65- 2.70 (m, 4H). MS (EI) for C2IHi9BrN4O2: 439 (MH+).
(Compound 261) 8-bromo-2-[( { r4-(methyloxy)phenyl1methyl| amino)methyll [ 1 "IbenzofuroB ,2- dlpyrimidin-4(3H)-one
[00302] 8-bromo-2-[({[4-
(methyloxy)phenyl]methyl}amino)methyl][l]benzofuro[3,2-d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 1, Compound 2, wherein piperidine was substituted with 4-methoxybenzylamine. Purification by preparative HPLC
(reverse-phase, acetonitrile/water with 0.1% formic acid), followed by concentration in vacuo and lyophilization afforded the title compound (22.5 mg). 1H NMR (400 MHz, de-DMSO): 8.18-8.20 (m, IH), 8.15 (s, IH), 7.80-7.85 (m, 2H), 7.28 (d, 2H), 6.87 (d, 2H), 3.75 (s, 2H), 3.72 (s, 3H). MS (EI) for Ci9Hi6BrN3O3: 414 (MH+). (Compound 262)
8-bromo-2- { r(2,3-dihydroxypropyl)aminolmethvU \ 1 lbenzofuro [3 ,2-dlpyrimidin- 4(3H)-one
[00303] 8-bromo-2-{[(2,3-dihydroxypropyl)amino]methyl}[l]benzofuro[3,2- d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 1, Compound 2, wherein piperidine was substituted with (±) 3-amino-l ,2-propanediol. Purification by preparative HPLC (reverse-phase, acetonitrile/water with 0.1% formic acid), followed by concentration in vacuo and lyophilization afforded the title compound (27.9 mg). 1H NMR (400 MHz, d6-DMSO): 8.24 (s, IH), 8.17-8.20 (m, IH), 7.78-7.84 (m, 2H), 3.75-3.83 (m, 2H), 3.57 (s, IH), 3.27-3.39 (m, 2H), 2.66-2.75 (m, 2H). MS (EI) for Ci4Hi4BrN3O4: 368 (MH+). (Compound 263)
8-bromo-2-[(2,3-dihydro-lH-inden-2-ylamino)methyll[llbenzofuro[3,2-dlpyrimidin- 4(3HVone
[00304] 8-bromo-2-[(2,3-dihydro-lH-inden-2-ylamino)methyl][l]benzofuro[3,2- d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 1,
Compound 2, wherein piperidine was substituted with 2-aminoindan. Purification by preparative HPLC (reverse-phase, acetonitrile/water with 0.1% formic acid), followed by concentration in vacuo and lyophilization afforded the title compound (19.9 mg). 1H NMR (400 MHz, d6-DMSO): 8.19-8.21 (m, IH), 7.78-7.84 (m, 2H), 7.16-7.22 (m, 2H), 7.09-7.14 (m, 2H), 3.84 (s, 2H), 3.55-3.66 (m, IH), 3.04-3.12 (m, 2H), 2.71-2.80 (m, 2H). MS (EI) for C20Hi6BrN3O2: 410 (MH+).
(Compound 268)
8-bromo-2-|Y { [2-(methyloxy)phenyl]methyl| amino)methyl] [ 1 "|benzofuro|"3 ,2- dlpyrimidin-4(3H)-one [00305] 8-bromo-2-[({[2-
(methyloxy)phenyl]methyl}amino)methyl][l]benzofuro[3,2-d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 1, Compound 2, wherein piperidine was substituted with 2-methoxybenzylamine. Purification by preparative HPLC (reverse-phase, acetonitrile/water with 0.1% formic acid), followed by concentration in vacuo and lyophilization afforded the title compound (10 mg). 1H NMR (400
MHz, d6-DMSO): 8.18 (m, IH), 7.81 (s, 2H), 7.33 (d, IH), 7.20 (t, IH), 6.94 (d, IH), 6.88 (t, IH), 3.79 (s, 3H), 3.77 (s, 2H), 3.72 (s, 2H). MS (EI) for Ci9Hi6BrN3O3: 415 (MH+).
(Compound 277) 8-bromo-2-( { r2-(methyloxy)ethyl1amino I methyl) [ 1 lbenzofuro [3 ,2-dlpyrimidin- 4(3HVone
[00306] 8-bromo-2-({[2-(methyloxy)ethyl]amino}methyl)[l]benzofuro[3,2- d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 1, Compound 2, wherein piperidine was substituted with 2-methoxyethylamine. Purification by preparative HPLC (reverse-phase, acetonitrile/water with 0.1% formic acid), followed by concentration in vacuo and lyophilization afforded the title compound (20.2 mg). NMR (400 MHz, d6-DMSO): 8.24 (s, IH), 8.17-8.19 (m, IH), 7.79-7.84 (m, 2H), 3.78 (s, 2H), 3.39-3.44 (m, 3H), 2.71-2.77 (m, 2H). MS (EI) for Ci4Hi4BrN3O3: 352 (MH+).
(Compound 278)
8-bromo-2- { [4-(3 -chlorophenvDpiperazin- 1 -yllmethyl) \ 1 lbenzofuro [3 ,2-dlpyrimidin- 4(3HVone
[00307] 8-bromo-2- { [4-(3-chlorophenyl)piperazin- 1 -yl]methyl} [ 1 ]benzofuro[3 ,2- d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 1, Compound 2, wherein piperidine was substituted with l-(3-chlorophenyl)piperazine. Purification by preparative HPLC (reverse-phase, acetonitrile/water with 0.1% formic acid), followed by concentration in vacuo and lyophilization afforded the title compound (34.6 mg). NMR (400 MHz, d6-DMSO): 8.22-8.23 (m, IH), 7.80-7.86 (m, 2H), 7.20 (t, IH), 6.87-6.95 (m, 2H), 6.75-6.79 (m, IH), 3.60 (s, 2H), 3.16-3.24 (m, 4H), 2.61-2.70 (m, 4H). MS (EI) for C2IHi8BrClN4O2: 473 (MH+).
(Compound 431) 8-bromo-2- { [ (2-piperidin- 1 -ylethvDaminolmethyU \ 1 lbenzofuro[3 ,2-dlpyrimidin- 4(3HVone
[00308] 8-bromo-2- { [(2-piperidin- 1 -ylethyl)amino]methyl} [ 1 ]benzofuro[3 ,2- d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 1, Compound 2, wherein piperidine was substituted with l-(2-aminoethyl)piperidine piperidine. 1H NMR (400 MHz, d6-DMSO): 8.07 (s, IH), 7.72 (m, 2H), 3.77 (s, 2H), 2.73 (t, 2H), 2.39 (t, 2H), 2.33 (m, 4H), 1.51 (m, 4H), 1.36 (m, 2H). MS (EI) for C18 H21 Br N4 O2: 406 MH+). EXAMPLE 2: (Scheme 1 except via Microwave promoted displacement)
(Compound 4) 8-bromo-2-[( { [4-(4-methylpiperazin- 1 - vOphenyllmethyl) amino)methyll [ 1 lbenzofuro[3 ,2-d1pyrimidin-4(3H)-one (acetate salt) [00309] To a solution of 8-bromo-2-(chloromethyl)benzofuro[3,2-d]pyrimidin-
4(3H)-one 6 (70 mg, 0.22 mmol) in 1.5 mL anhydrous DMF was added (4-(4- methylpiperazin-l-yl)phenyl)methanamine (50 mg, 0.22 mmol) and Cs2CO3 (143 mg, 0.44 mmol). The reaction mixture was heated to 85 0C at 150 W for 10 minutes in a CEM-Discover microwave reactor. The reaction mixture was filtered and concentrated in vacuo. Purification by preparative HPLC, followed by concentration in vacuo and lyophilization afforded the title compound (18.2 mg, 17%) as a white solid. 1H NMR (400 MHz, d6-DMSO): 8.16 (m, IH), 7.79 (m, 2H), 7.19 (d, 2H), 6.86 (d, 2H), 3.72 (s, 2H), 3.68 (s, 2H), 3.07 (m, 4H), 2.50 (m, 4H), 2.21 (s, 3H), 1.90 (s, 3H); MS (EI) for C23H24BrN5O2: 482 (MH+). (Compound 8)
8-bromo-2-[(piperidin-4-ylamino)methyll[llbenzofuro[3,2-dlpyrimidin-4(3H)-one [00310] 8-bromo-2-[(piperidin-4-ylamino)methyl][l]benzofuro[3,2-d]pyrimidin- 4(3H)-one was synthesized in a manner similar to Example 2, wherein (4-(4- methylpiperazin-l-yl)phenyl)methanamine was substituted with tert-butyl 4- aminopiperidine-1-carboxylate. Purification by preparative HPLC, followed by concentration in vacuo and lyophilization afforded tert-butyl 8-bromo-2-[(piperidin-4- ylamino)methyl][l]benzofuro[3,2-d]pyrimidin-4(3H)-one-carboxylate. Tert-butyl 8- bromo-2-[(piperidin-4-ylamino)methyl][l]benzofuro[3,2-d]pyrimidin-4(3H)-one- carboxylate was taken in 4 mL methanol and 2mL of 4N HCl/dioxanes. After 18 hours, the reaction mixture was concentrated in vacuo and purified by preparative HPLC, followed by concentration in vacuo and lyophilization to afford the title compound (5 mg, 6%) as a white solid. 1H NMR (400 MHz, d6-DMSO): 8.12 (m, IH), 7.74 (m, 2H), 3.75 (s, 2H), 2.64 (m, 3H), 2.54 (m, 2H), 1.9 (s, 3H), 1.85 (m, 2H), 1.30 (m, 2H); MS (EI) for Ci6Hi7BrN4O2: 377 (MH+). (Compound 20) l-[(8-bromo-4-oxo-3,4-dihydro[llbenzofuro[3,2-dlpyrimidin-2-yl)methyll-N-[3- (dimethylamino)propyllprolinamide (HCl salt)
[00311] l-[(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2- yl)methyl]-N-[3-(dimethylamino)propyl]prolinamide was synthesized in a manner similar to Example 2, wherein (4-(4-methylpiperazin-l-yl)phenyl)methanamine was substituted with N-(3-(dimethylamino)propyl)pyrrolidine-2-carboxamide. The crude reaction mixture was purified by preparative HPLC (reverse-phase, acetonitrile/water with 10 mM ammonium acetate) and (reverse-phase, 0.1% TFA in acetonitrile/0.05% TFA in water) to afford the title compound (3.7 mg, 4.5%) as a white solid. 1H NMR (400 MHz, de-DMSO): 8.5 (br s, IH), 8.19 (m, IH), 7.87 (m, 2H), 3.36-3.12 (s, 2 H, overlapped), 3.21-3.05 (m, 7 H), 2.71 (s, 6H), 1.77 (m, 6H); MS (EI) for C2IH26BrN5O3: 476 (MH+). (Compound 6)
8-bromo-2-( 1 H-imidazol- 1 -ylmethyl)[ 1 ]benzofuro[3 ,2-d]pyrimidin-4(3H)-one [00312] 8-bromo-2-(lH-imidazol-l-ylmethyl)[l]benzofuro[3,2-d]pyrimidin-4(3H)- one was synthesized in a manner similar to Example 2, wherein (4-(4- methylpiperazin-l-yl)phenyl)methanamine was substituted with imidazole.
Purification by preparative HPLC resulting in 14 mg (13% Yield) of 8-bromo-2-(lH- imidazol-l-ylmethyl)[l]benzofuro[3,2-d]pyrimidin-4(3H)-one as the HCl salt. 1H NMR (400MHz, d6-DMSO+D2O): 9.22 (s, IH), 8.01 (s,lH), 7.85 (m, 3H), 7.78 (s, IH), 5.61 (s, 2H); MS (EI) for Ci4H9BrN4O2: 345:347 (Bromine isotope MH+). (Compound 9)
8-bromo-2-({4-[3-(dimethylamino)propyllpiperazin-l-yl|methyl)[llbenzofuro[3,2- dlpyrimidin-4(3H)-one
[00313] 8-bromo-2-( {4-[3-(dimethylamino)propyl]piperazin- 1 - yl}methyl)[l]benzofuro[3,2-d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 2, wherein (4-(4-methylpiperazin-l-yl)phenyl)methanamine was substituted with commercially available l-(3-dimethyl aminopropyl)-piperazine. Purification by preparative HPLC resulting in 54 mg (37% Yield) of 8-bromo-2-({4- [3-(dimethylamino)propyl]piperazin- 1 -yl}methyl)[ 1 ]benzofuro[3,2-d]pyrimidin- 4(3H)-one as the HCl salt. 1U NMR (400MHz, d6-DMSO + D2O): 8.20 (s, IH), 7.90 (s,2H), 3.44 (m, 6H), 3.05 (m, 6H), 2.78 (s, 6H), 1.98 (m, 2 H); MS (EI) for C20H26BrN5O2: 448 : 450 (Bromine isotope MH+).
(Compound 10)
8-bromo-2-[( { r2-(dimethylamino)phenyl1methvU amino)methyll [ 1 lbenzofuro[3 ,2- d"|pyrimidin-4(3H)-one [00314] 8-bromo-2-[( { [2-(dimethylamino)phenyl]methyl} amino)methyl] [I]- benzofuro[3,2-d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 2, wherein (4-(4-methylpiperazin-l-yl)phenyl)methanamine was substituted with commercially available N-[2-(aminomethyl) phenyl]-N,N- dimethylamine. Purification by preparative HPLC resulting in 27 mg (24% Yield) of 8-bromo-2-[( { [2-(dimethylamino)phenyl]methyl} amino)methyl] [ 1 ]benzofuro[3 ,2- d]pyrimidin-4(3H)-one as the HCl salt. 1H NMR (400MHz, d6-DMSO + D2O): 8.22 (s, IH), 7.86 (m,2H), 7.50 (m, 4H), 7.28 (t, IH), 4.55 (s, 2H), 4.32 (s, 2 H), 2.79 (s, 6H); MS (EI) for C20Hi9BrN4O2: 427 : 429 (Bromine isotope MH+). (Compound 14)
8-bromo-2-|Y { [3-(4-methylpiperazin- 1 -yl)phenyl]methyl| amino)- methyll [ 1 lbenzofuro [3 ,2-d1pyrimidin-4(3H)- one [00315] 8-bromo-2-[({[3-(4-methylpiperazin-l- yl)phenyl]methyl} amino)methyl] [ 1 ]benzofuro[3 ,2-d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 2, wherein (4-(4-methylpiperazin-l- yl)phenyl)methanamine was substituted with commercially available l-[3-(4-methyl piperazin-l-yl)phenyl]methanamine. Preparative HPLC purification gave 27 mg (25% Yield) of 8-bromo-2-[({[3-(4-methylpiperazin-l- yl)phenyl]methyl}amino)methyl][l]benzofuro[3,2-d]pyrimidin-4(3H)-one. 1H NMR (400MHz, de-DMSO): 11.21 (br s, IH), 10.20 (br s,2H), 8.20 (s, IH), 7.89 (m, 2H), 7.34 (m, 2H), 7.08 (d, 2 H), 4.30 (d, 4H), 3.89 (d, 2H), 3.50 (d, 2H), 3.19 (m, 4H), 2.81 (d, 3H), 2.48 (s, 3H); MS (EI) for C23H24BrN5O2: 482:484 (Bromine isotope MH+). (Compound 17)
8-bromo-2- { [3-(dimethylamino)pyrrolidin- 1 -yl]methyl| [ 1 lbenzofuro [3,2- dlpyrimidin-4(3H)-one [00316] 8-bromo-2- { [3-(dimethylamino)pyrrolidin- 1 -yljmethyl} [ 1 ]benzofuro[3,2- d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 2, wherein (4-(4-methylpiperazin-l-yl)phenyl)methanamine was substituted with commercially available 3-(dimethylamino)pyrrolidine. Preparative HPLC purification gave 32 mg
(37% Yield) of 8-bromo-2-{[3-(dimethylamino)pyrrolidin-l- yl]methyl}[l]benzofuro[3,2-d]pyrimidin-4(3H)-one. 1H NMR (400MHz, d6-DMSO +
D2O): 8.30 (s, IH), 7.85 (s,2H), 4.19 (s, 2H), 3.40 (m, 4H), 3.15 (m, IH), 2.81 (s, 6 H), 2.38 (m, IH), 2.19 (m, IH); MS (EI) for Ci7Hi9BrN4O2: 391 :393 (Bromine isotope MH+).
(Compound 18)
8-bromo-2- { [(2-chlorophenyl)(methyl)amino"|methyU [ 1 lbenzofuro[3 ,2-d]pyrimidin- 4(3H)-one [00317] 8-bromo-2-{[(2-chlorophenyl)(methyl)amino]methyl} [l]benzofuro[3,2- d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 2, wherein (4-(4-methylpiperazin-l-yl)phenyl)methanamine was substituted with commercially available 2-chloro-N-Methyl aniline. Precipitation gave 15 mg (37% Yield) of 8- bromo-2-{[(2-chlorophenyl)(methyl)amino]methyl}[l]benzofuro[3,2-d]pyrimidin- 4(3H)-one. 1H NMR (400MHz, d6-DMSO): 12.64 (s, IH), 8.15 (s,lH), 7.82 (m, 2H), 7.42 (d, 1H),7.38 (d, IH), 7.31 (t, IH), 7.15 (td, IH), 4.36 (s, 2 H), 2.92 (s, 3H); MS (EI) for Ci8Hi3BrClN3O2: 418:420 (Bromine isotope MH+). (Compound 25)
2-{4-[(8-bromo-4-oxo-3,4-dihydro[11benzofuro[3,2-d]pyrimidin-2- yl)methyl"|piperazin- 1 -yl| -N,N-dimethylacetamide [00318] 2-{4-[(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2- yl)methyl]piperazin-l-yl}-N,N-dimethylacetamide was synthesized in a manner similar to Example 2, wherein (4-(4-methylpiperazin-l-yl)phenyl)methanamine was substituted with commercially available [piperazino-N, N-dimethyl amide.
Preparative HPLC gave 40 mg (41% Yield) of 2-{4-[(8-bromo-4-oxo-3,4- dihydro [ 1 ]benzofuro [3 ,2-d]pyrimidin-2-yl)methyl]piperazin- 1 -yl} -N5N- dimethylacetamide. 1H NMR (400MHz, d6-DMSO): 8.21 (s, IH), 7.80 (m,2H), 3.55 (br s, 4H), 3.15 (s, 3H), 3.00 (s, 3H), 2.82 (s, 3H), 2.40 (br s, 8 H), 1.90 (s, 2H); MS
(EI) for Ci9H22BrN5O3: 448:450 (Bromine isotope MH+).
(Compound 26)
8-bromo-2- { r4-(N,N-diethylglvcvDpiperazin- 1 -yllmethyl) \ 1 lbenzofuroB ,2- dlpyrimidin-4(3H)-one [00319] 8-bromo-2-{[4-(N,N-diethylglycyl)piperazin-l- yl]methyl}[l]benzofuro[3,2-d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 2, wherein (4-(4-methylpiperazin-l-yl)phenyl)methanamine was substituted with 2-(diethylamino)-l-(piperazin-l-yl)ethanone. Preparative HPLC gave 40 mg (41% Yield) of 8-bromo-2-{[4-(N,N-diethylglycyl)piperazin-l- yl]methyl} [l]benzofuro[3,2-d]pyrimidin-4(3H)-one. 1H NMR (400MHz, d6-DMSO): 8.35 (s, IH), 7.85 (m,2H), 4.43 (br s, 2H), 4.29 (br s, 2H), 3.90 (br s, 2H), 3.79 (br s, 2H), 3.43 (m, 4H), 3.20 (m, 4 H), 1.25 (t, 6H); MS (EI) for C2IH26BrN5O3: 476:478 (Bromine isotope MH+).
(Compound 75) 8-bromo-2-( { [4-(4-methylpiperazin- 1 -yPphenyl] amino I methyl) [ 1 ]benzofuro [3,2- dlpyrimidin-4(3H)-one [00320] 8-bromo-2-( { [4-(4-methylpiperazin- 1 - yl)phenyl] amino } methyl) [ 1 Jbenzofuro- [3 ,2-d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 2, wherein (4-(4-methylpiperazin-l- yl)phenyl)methanamine was substituted with 4-(4-methylpiperazino)aniline. Purification by preparative, followed by concentration in vacuo and lyophilization afforded the title compound (14 mg, 23%) as a white solid. 1H NMR (400 MHz, d6- DMSO): 8.21 (s, IH), 7.85 (s, 2H), 6.88 (d, 2H), 6.71 (d, 2H), 4.32 (s, 2H), 3.22 (br q, 4H), 2.90 (br t, 4H), 2.80 (d, 3H); MS (EI) for C22H22BrN5O2: 468.1 :470.1 (Bromine isoptope MH+).
(Compound 76)
8-bromo-2-({r3-(dimethylamino)phenyllamino|methyl)πibenzofuror3,2- dlpyrimidin-4(3H)-one
[00321] 8-bromo-2-({[3-(dimethylamino)phenyl]amino}methyl)[l]benzofuro[3,2- d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 2, wherein (4-(4-methylpiperazin-l-yl)phenyl)methanamine was substituted with N,N-dimethyl- m-phenylene diamine HCl. Purification by preparative HPLC (reverse-phase, acetonitrile/water with 0.01% ammonium acetate), followed by concentration in vacuo and lyophilization afforded the title compound (6 mg, 12%) as a white solid. 1H NMR (400 MHz, d6-DMSO): 8.20 (s, IH), 7.81 (s, 2H), 6.88 (t, IH), 6.09 (s, IH), 6.02 (m, 2H), 5.90 (br s, IH), 4.29 (br s, 2H), 2.80 (s, 6H); MS (EI) for Ci9Hi7BrN4O2: 412:414 (Bromine isoptope MH+). (Compound 81)
8-bromo-2-({4-[2-(dimethylamino)ethyllpiperazin-l-yl|methyl)[llbenzofuro[3,2- dlpyrimidin-4(3H)-one
[00322] 8-bromo-2-( {4-[2-(dimethylamino)ethyl]piperazin- 1 - yl}methyl)[l]benzofuro-[3,2-d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 2, wherein (4-(4-methylpiperazin-l-yl)phenyl)methanamine was substituted with N,N-dimethyl-2-(piperazin-l-yl)ethanamine. The reaction mixture was concentrated in vacuo and purified by preparative HPLC. Concentration in vacuo and lyophilization afforded the title compound (27.5 mg, 22%). 1H NMR (400 MHz, de-DMSO): 8.20 (m, IH), 7.85 (m, 2H), 3.56 (m, 4H), 3.15 (m, 4H), 2.74 (s, 6H), 2.67 (m, 3H), 2.57 (m, 3H). MS (EI) for Ci9H24BrN5O2: 434.1 (MH+).
(Compound 82)
8-bromo-2- { r4-(2-morpholin-4-ylethyl)piperazin- 1 -yllmethyl} [ 1 lbenzofuroB ,2- dlpyrimidin-4(3H)-one [00323] 8-bromo-2-{[4-(2-morpholin-4-ylethyl)piperazin-l- yl]methyl}[l]benzofuro[3,2-d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 2, wherein (4-(4-methylpiperazin-l-yl)phenyl)methanamine was substituted with 4-(2-(piperazin-l-yl)ethyl)morpholine. The reaction mixture was concentrated in vacuo and purified by preparative HPLC. Concentration in vacuo and lyophilization afforded the title compound (15 mg, 31.5%). 1H NMR (400 MHz, d6- DMSO): 8.2 (m, IH), 7.81 (m, 2H), 3.53 (m, 6 H), 3.50 (s, 2H), 2.38 (m, 14 H). MS (EI) for C2IH26BrN5O3: 476.3 (MH+).
(Compound 100) 8-bromo-2-[(2 -methyl- lH-imidazol- 1 -vDmethyli [ 1 lbenzofuro[3 ,2-dlpyrimidin- 4(3H)-one
[00324] 8-bromo-2-[(2-methyl- lH-imidazol- 1 -yl)methyl] [ 1 ]benzofuro[3 ,2- d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 2, wherein (4-(4-methylpiperazin-l-yl)phenyl)methanamine was substituted with 2-methyl imidazole. Purification by preparative HPLC afforded the title compound (15 mg, 13%) as a white solid. 1H NMR (400 MHz, d6-DMSO): 8.05 (s, IH), 7.79 (s, 2H), 7.16 (s, IH), 6.76 (s, 2H), 5.12 (s, 2H), 2.36 (s, 3H), 1.91 (s, 3H); MS (EI) for Ci5HnBrN4O2: 359:361 (Bromine isoptope MH+).
(Compound 103) l-[(8-bromo-4-oxo-3,4-dihydro[llbenzofuro[3,2-dlpyrimidin-2- yl)methyl"|pyrrolidine-3-carboxylic acid
[00325] l-[(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2- yl)methyl]pyrrolidine-3-carboxylic acid was synthesized in a manner similar to Example 2, wherein (4-(4-methylpiperazin-l-yl)phenyl)methanamine was substituted with pyrrolidine-3-carboxylic acid. Purification by preparative HPLC (reverse-phase, acetonitrile/water with 0.01% ammonium acetate), followed by concentration in vacuo and lyophilization afforded the title compound (7 mg, 5.6%). 1H NMR (400 MHz, de-DMSO): 8.19 (m, IH), 7.78 (m, 2H), 3.65 (m, 2H), 2.90 (m, 2H), 2.78 (m, IH), 2.66 (m, 2H), 1.96 (m, 2H). MS (EI) for Ci6Hi4BrN3O4: 392 (MH+). (Compound 104)
8-bromo-2-|Y { [4-(dimethylamino)phenyl]methyU amino)methyl] [ 1 ]benzofuro[3 ,2- d1pyrimidin-4(3H)-one [00326] 8-bromo-2-[({[4-
(dimethylamino)phenyl]methyl} amino)methyl] [ 1 ]benzofuro-[3 ,2-d]pyrimidin-4(3H)- one was synthesized in a manner similar to Example 2, wherein (4-(4- methylpiperazin-l-yl)phenyl)methanamine was substituted with 4-(aminomethyl)- N,N-dimethylaniline. The reaction mixture was concentrated in vacuo and purified by preparative HPLC. Concentration in vacuo and lyophilization afforded the title compound (3.9 mg, 3%). 1H NMR (400 MHz, d6-DMSO): 8.17 (m, IH), 7.80 (m, 2H), 7.17 (d, 2H), 6.65 (d, 2H), 3.74 (s, 2H), 3.68 (s, 2H), 2.84 (s, 6H). MS (EI) for C20Hi9BrN4O2: 427 (MH+). (Compound 368) l-[(8-bromo-4-oxo-3,4-dihydro[llbenzofuro[3,2-dlpyrimidin-2-yl)methyll-3- hydroxypyrrolidine-3 -carboxylic acid
[00327] l-[(8-Bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2- yl)methyl] -3 -hydroxypyrrolidine-3 -carboxylic acid was synthesized in a manner similar to was synthesized in a manner similar to Example 2 wherein (4-(4- methylpiperazin-l-yl)phenyl)methanamine was substituted with 3-hydroxy-3- pyrrolidinecarboxylic acid. 1H NMR (400 MHz, d6-DMSO): 8.21 (m, IH), 7.78 (m, 2H), 3.68 (dd, 2H), 3.00 (d, IH), 2.90-2.62 (m, 2H), 2.45 (m, IH), 2.20-2.03 (m, IH), 1.90-1.82 (m, IH); MS (EI) for Ci6Hi4BrN3O5: 408 (MH+). (Compound 108)
2-{[(3S)-3-aminopyrrolidin-l-yllmethyl|-8-bromo[llbenzofuro[3,2-dlpyrimidin-
4(3HVone [00328] 2-{[(3S)-3-aminopyrrolidin-l-yl]methyl}-8-bromo[l]benzofuro[3,2- d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 2, wherein (4-(4-methylpiperazin-l-yl)phenyl)methanamine was substituted with (s)-(-)-3-(Boc amino) pyrrolidine. Purification by flash chromatography (60:40 EtOAc :Hexanes to
100% EtOAc) afforded the Boc protected title compound. The intermediate was then taken up in 1.0 mL MeOH and 0.8 mL 4N HCl/dioxane and stirred at RT for 5 hr.
The precipitate was filtered and rinsed with MeOH 3X to afford the title compound as an HCl salt (39 mg, 34%). 1H NMR (400 MHz, d6-DMSO): 8.29 (s, IH), 7.88 (s,
2H), 4.60 (br s, 2H), 4.01 (br s, IH), 2.45 (m, IH), 2.15 (m, IH); MS (EI) for
Ci5Hi5BrN4O2: 363:365 (Bromine isoptope MH+). (Compound 109)
2- {[(3R)-3-aminopyrroridin- 1 -yl]methyl| -8-bromo[ 1 lbenzofuro[3,2-dlpyrimidin- 4(3HVone [00329] 2-{[(3R)-3-aminopyrrolidin-l-yl]methyl}-8-bromo[l]benzofuro[3,2- d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 2, wherein (4-(4-methylpiperazin-l-yl)phenyl)methanamine was substituted with (R)-(-)-3-(Boc amino) pyrrolidine. Purification by flash chromatography (60:40 EtOAc :Hexanes to 100% EtOAc) afforded the Boc protected title compound. The intermediate was then taken up in 1.0 mL MeOH and 0.8 mL 4N HCl/dioxane and stirred at RT for 5 hr. The precipitate was filtered and rinsed with MeOH 3X to afford the title compound as an HCl salt (39 mg, 34%). 1H NMR (400 MHz, d6-DMSO): 8.29 (s, IH), 7.88 (s, 2H), 4.60 (br s, 2H), 4.01 (br s, IH), 2.45 (m, IH), 2.15 (m, IH); MS (EI) for Ci5Hi5BrN4O2: 363:365 (Bromine isoptope MH+).
(Compound 113) 8-bromo-2-({4-r2-(lH-imidazol-l-yl)ethyllpiperazin-l-vUmethyl)rilbenzofuror3,2- dlpyrimidin-4(3H)-one
[00330] 8-bromo-2-( {4-[2-( 1 H-imidazol- 1 -yl)ethyl]piperazin- 1 - yl}methyl)[l]benzofuro[3,2-d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 2, wherein (4-(4-methylpiperazin-l-yl)phenyl)methanamine was substituted with l-(2-Imidazol-l-yl ethyl)-piperazine. Purification by preparative HPLC afforded the title compound (63 mg, 43%). 1U NMR (400 MHz, d6-DMSO): 8.21 (s, IH), 7.80 (m, 2H), 7.68 (s, IH), 7.21 (s, IH), 6.90 (s, IH), 4.11 (m, 2H), 3.58 (s, 2H), 2.66 (m, 2H), 2.44 (m, 2H), 1.93 (s, IH); MS (EI) for C20H2IBrN6O2: 457:459 (Bromine isoptope MH+). (compound 114)
8-bromo-2-|Y4,4-difluoropiperidin- 1 -yQmethyl] [ 1 ]benzofuro|"3 ,2-d]pyrimidin-4(3H)- one
[00331] 8-bromo-2-[(4,4-difluoropiperidin- 1 -yl)methyl] [ 1 ]benzofuro[3 ,2- d]pyrimidin-4(3H)-one in a manner similar to Example 2, wherein (4-(4- methylpiperazin-l-yl)phenyl)methanamine was substituted with 4,4- difluoropiperidine. The reaction mixture was concentrated in vacuo and purified by preparative HPLC to afford the title compound (15 mg, 15%). 1H NMR (400 MHz, de-DMSO): 8.21 (m, IH), 7.83 (m, 2H), 3.63 (s, 2H), 2.66 (m, 4H), 2.0 (m, 4H). MS
(EI) for Ci6Hi4BrF2N3O2: 398 (MH+). (Compound 118)
8-bromo-2- { [3-(methylamino)pyrrolidin- 1 -yl]methyl| [ 1 lbenzofuro[3 ,2-d]pyrimidin- 4(3HVone
[00332] 8-bromo-2- { [3-(methylamino)pyrrolidin- 1 -yl]methyl} [ 1 ]benzofuro[3 ,2- d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 2, wherein (4-(4-methylpiperazin-l-yl)phenyl)methanamine was substituted with 3-(N-tert- butoxycarbonyl-N-methyl amino)pyrrolidine. Purification by flash chromatography (60:40 EtOAc:Hexanes to 100% EtOAc) afforded the Boc protected title compound. The intermediate was then taken up in 3.0 mL MeOH and 2.0 mL 4N HCl/dioxane and stirred at RT for 4 hr. The precipitate was filtered and rinsed with MeOH 3X to afford the title compound as an HCl salt (103 mg, 43%). 1U NMR (400 MHz, d6- DMSO): 8.25 (s, IH), 7.88 (s, 2H), 3.85 (br s, 2H), 3.44 (s, 3H), 2.60 (m, 4H); MS (EI) for Ci6Hi7BrN4O2: 379:381 (Bromine isoptope MH+).
(Compound 149) 8-bromo-2-IY2-ethyl- 1 H-imidazol- 1 -vDmethyll \ 1 ibenzofuroB .2-d1pyrimidin-4(3HV one
[00333] 8-bromo-2-[(2-ethyl-lH-imidazol-l-yl)methyl][l]benzofuro[3,2- d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 2, wherein (4-(4-methylpiperazin-l-yl)phenyl)methanamine was substituted with 2-ethyl imidazole. Purification by preparative HPLC afforded the title compound (16 mg, 13%) as a white solid. 1H NMR (400 MHz, d6-DMSO): 8.04 (s, IH), 7.83 (m, 2H), 7.21 (s, IH), 6.85 (s, IH), 5.19 (s, 2H), 2.74 (q, 2H), 1.23 (t, 3H); MS (EI) for Ci6Hi3BrN4O2: 373:375 (Bromine isoptope MH+).
(Compound 150) 8-bromo-2-[(4-ethylpiperazin- 1 -yDmethyl] [ 1 ]benzofuro[3 ,2-dlpyrimidin-4(3H)-one [00334] 8-bromo-2-[(4-ethylpiperazin- 1 -yl)methyl] [ 1 ]benzofuro[3 ,2-d]pyrimidin- 4(3H)-one was synthesized in a manner similar to Example 2, wherein (4-(4- methylpiperazin-l-yl)phenyl)methanamine was substituted with 1-ethylpiperazine. Purification by preparative HPLC, followed by concentration in vacuo and lyophilization afforded the title compound (65 mg, 53%) as a white solid. 1H NMR (400 MHz, de-DMSO): 8.21 (s, IH), 7.81 (m, 2H), 3.46 (s, 2H), 3.06 (br s, 4H), 2.45 (m, 4H), 2.32 (q, 2H), 1.90 (s, 2H), 0.973 (t, 3H); MS (EI) for CnHi9BrN4O2: 391 :393 (Bromine isoptope MH+). (Compound 151)
[00335] 8-bromo-2-( (4-r2-(methyloxy)ethyllpiperazin- 1 - vUmethyl)rilbenzofuror3,2-dlpyrimidin-4(3H)-one
8-bromo-2-({4-[2-(methyloxy)ethyl]piperazin-l-yl}methyl)[l]benzofuro[3,2- d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 2, wherein (4-(4-methylpiperazin-l-yl)phenyl)methanamine was substituted with l-(2- methoxyethyl)-piperazine. Purification by preparative HPLC, followed by concentration in vacuo and lyophilization afforded the title compound (72 mg, 55%). 1H NMR (400 MHz, d6-DMSO): 8.19 (s, IH), 7.80 (s, 2H), 3.39 (s, 2H), 3.37 (br s, 8H), 3.18 (s, 3H), 2.42 (t, 4H); MS (EI) for Ci8H2IBrN4O3: 421 :423 (Bromine isoptope MH+).
(Compound 165)
8-bromo-2- { r(pyridin-4-ylmethyl)aminolmethyl| \ 1 lbenzofuro[3 ,2-dlpyrimidin- 4(3HVone [00336] 8-bromo-2-{[(pyridin-4-ylmethyl)amino]methyl} [l]benzofuro[3,2- d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 2, wherein (4-(4-methylpiperazin-l-yl)phenyl)methanamine was substituted with 4-Amino methyl pyridine. Purification by preparative HPLC, followed by concentration in vacuo and lyophilization afforded the title compound (21 mg, 17%). 1H NMR (400 MHz, de-DMSO): 8.48 (d, 2H), 8.19 (s, IH), 7.82 (m, 2H), 7.38 (d, 2H), 3.81 (s, 2H), 3.76 (s, 2H); MS (EI) for Ci7Hi3BrN4O2: 385:387 (Bromine isoptope MH+).
(Compound 166)
8-bromo-2- { r(2-pyridin-4-ylethyl)amino"|methyl| \ 1 lbenzofuroB ,2-dlpyrimidin- 4(3H)-one [00337] 8-bromo-2-{[(2-pyridin-4-ylethyl)amino]methyl} [l]benzofuro[3,2- d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 2, wherein (4-(4-methylpiperazin-l-yl)phenyl)methanamine was substituted with 4-(2- aminoethyl)pyridine. Purification by preparative HPLC, followed by concentration in vacuo and lyophilization afforded the title compound (14 mg, 11%). 1H NMR (400 MHz, de-DMSO): 8.74 (d, 2H), 8.28 (s, IH), 7.87 (s, 2H), 7.83 (d, 2H), 4.32 (s, 2H), 3.54 (t, 2H), 3.30 (t, 2H); MS (EI) for Ci8Hi5BrN4O2: 399:401 (Bromine isoptope MH+). (Compound 177)
N-2-[(8-bromo-4-oxo-3,4-dihydro[llbenzofuro[3,2-dlpyrimidin-2-yl)methyll-2- methylalaninamide
[00338] N-2-[(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2- yl)methyl]-2-methylalaninamide was synthesized in a manner similar to Example 2, wherein (4-(4-methylpiperazin-l-yl)phenyl)methanamine was substituted with methyl-α-aminoisobutyrate. Purification by preparative HPLC, afforded methyl 2- ((8-bromo-4-oxo-3,4-dihydrobenzofuro[3,2-d]pyrimidin-2-yl)methylamino)-2- methylpropanoate which was then taken up in 2 mL of 7M ammonia in methanol and heated to 100 0C overnight in a sealed vessel. The resulting precipitate was filtered and dried affording the title compound (7.0mg, 47%). 1H NMR (400 MHz, d6-DMSO + D2O): 8.25 (s, IH), 7.83 (m, 2H), 3.73 (s, 2H), 1.31 (s, 6H); MS (EI) for Ci5Hi5BrN4O3: 379:381 (Bromine isoptope MH+).
(Compound 189) N-r(8-bromo-4-oxo-3,4-dihvdrorilbenzofuror3,2-dlpyrimidin-2-yl)methyll-2- methylalanine
[00339] N-[(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2- yl)methyl]-2-methylalanine was synthesized in a manner similar to Example 2, wherein (4-(4-methylpiperazin-l-yl)phenyl)methanamine was substituted with methyl-α-aminoisobutyrate. Purification by preparative HPLC, afforded methyl 2- ((8-bromo-4-oxo-3,4-dihydrobenzofuro[3,2-d]pyrimidin-2-yl)methylamino)-2- methylpropanoate which was then taken up in 1.0 mL of 2N LiOH and heated to 50 0C for 1.5 hr. The reaction was acidified to pH 4 with IN HCl and extracted with EtOAc, dried with Na2SO4 and concentrated to afford the title compound (3.0mg, 20%). 1H NMR (400 MHz, d6-DMSO + D2O): 8.25 (s, IH), 7.86 (m, 2H), 4.15 (m, 2H), 1.43 (s, 6H); MS (EI) for Ci5Hi4BrN3O4: 380:382 (Bromine isoptope MH+).
(Compound 190)
8-bromo-2- ([(U -dimethyl-2-pyrrolidin- 1 -ylethyl)amino"|methyl| [ 1 lbenzofuro[3 ,2- dlpyrimidin-4(3H)-one [00340] 8-bromo-2- {[(1,1 -dimethyl-2-pyrrolidin- 1- ylethyl)amino]methyl}[l]benzofuro[3,2-d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 2, wherein (4-(4-methylpiperazin-l- yl)phenyl)methanamine was substituted with 1,1 -dimethyl-2-pyrrolidin- 1- ylethyl)amine. Purification by preparative HPLC, followed by concentration in vacuo and lyophilization afforded the title compound (58mg, 23%). 1H NMR (400 MHz, d6- DMSO): 8.10 (d, IH), 7.75 (m, 2H), 3.70 (s, 2H), 2.69 (br s, 2H), 2.56 (s, IH), 2.48 (br s, 2H), 1.88 (br s, IH), 1.76 (br s, 4H), 1.11 (s, 6H); MS (EI) for Ci9H23BrN4O2: 419:421 (Bromine isoptope MH+).
(Compound 307)
8-bromo-2- { [(3R)-3-fluoropyrrolidin- 1 -yl"|methyl| [ 1 "|benzofuro|"3 ,2-d]pyrimidin- 4(3H)-one [00341] 8-bromo-2- {[(3R)-3-fluoropyrrolidin- 1 -yl]methyl} [ 1 ]benzofuro[3,2- d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 2, wherein (4-(4-methylpiperazin-l-yl)phenyl)methanamine was substituted with R-(-)-3- fluoropyrrolidine HCl. Purification by silica gel chromatography afforded the title compound (18mg, 16%). 1H NMR (400 MHz, d6-DMSO): 8.21 (d, IH), 7.83 (m, 2H), 5.29 (t, 0.5H), 5.15 (t, 0.5H), 4.12 (m, IH), 3.69 (s, 2H), 2.88 (m, 3H), 2.15 (m, IH), 1.90 (m, IH); MS (EI) for Ci5Hi3BrFN3O2: 366:367 (Bromine isoptope MH+).
(Compound 308)
8-bromo-2- { [(3 S)-3 -fluoropyrrolidin- 1 -yl]methyl| [ 1 Ibenzofuro [3 ,2-d]pyrimidin- 4(3HVone [00342] 8-bromo-2- { [(3 S)-3 -fluoropyrrolidin- 1 -yl]methyl} [ 1 ]benzofuro [3 ,2- d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 2, wherein (4-(4-methylpiperazin-l-yl)phenyl)methanamine was substituted with S-(-)-3- fluoropyrrolidine HCl. Purification by silica gel chromatography afforded the title compound (45mg, 40%). 1H NMR (400 MHz, d6-DMSO): 8.21 (d, IH), 7.83 (m, 2H), 5.29 (t, 0.5H), 5.15 (t, 0.5H), 4.12 (m, IH), 3.69 (s, 2H), 2.88 (m, 3H), 2.15 (m, IH), 1.90 (m, IH); MS (EI) for Ci5Hi3BrFN3O2: 366:367 (Bromine isoptope MH+).
(Compound 317)
8-bromo-2-[(3-fluoropiperidin-l-yl)methyll[llbenzofuro[3,2-dlpyrimidin-4(3H)-one [00343] 8-bromo-2-[(3-fluoropiperidin-l-yl)methyl][l]benzofuro[3,2-d]pyrimidin- 4(3H)-one was synthesized in a manner similar to Example 2, wherein (4-(4- methylpiperazin-l-yl)phenyl)methanamine was substituted with 3-fluoropiperidine HCl. Purification by silica gel chromatography twice afforded the title compound (18 mg, 40%). 1H NMR (400 MHz, d6-DMSO): 8.21 (m, IH), 7.83 (m, 2H), 4.74 (m, 0.5H), 4.61 (m, 0.5H), 3.58 (s, 2H), 2.09 (m, IH), 2.51 (m, 2H), 1.80 (m, 2H), 1.50 (m, 2H); MS (EI) for Ci6Hi5BrFN3O2: 380:382 (Bromine isoptope MH+).
(Compound 318)
8-bromo-2-[(3 ,3-difluoropyrrolidin- 1 -vDmethyll [ 1 lbenzofuro[3 ,2-d1pyrimidin-4(3H)- one
[00344] 8-bromo-2-[(3 ,3-difluoropyrrolidin- 1 -yl)methyl] [ 1 ]benzofuro [3,2- d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 2, wherein (4-(4-methylpiperazin-l-yl)phenyl)methanamine was substituted with 3,3- difluoroazetidine HCl. Purification by silica gel chromatography twice afforded the title compound (21 mg, 17%). 1H NMR (400 MHz, d6-DMSO): 8.21 (m, IH), 7.83 (m, 2H), 3.71 (s, 2H), 3.08 (t, 2H), 2.88 (t, 2H), 2.28 (heptet, 2H); MS (EI) for Ci5Hi2BrF2N3O2: 380:382 (Bromine isoptope MH+).
EXAMPLE 3: (Scheme 1 - generating ethers as a product)
(Compound 15) 8-bromo-2-[(phenyloxy)methyll[llbenzofuro[3,2-dlpyrimidin-4(3H)-one
[00345] To a solution of 8-bromo-2-(chloromethyl)benzofuro[3,2-d]pyrimidin- 4(3H)-one 6 (111 mg, 0.35 mmol) in 5mL anhydrous DMF was added phenol (200 mg, 2.12 mmol) and K2CO3 (292 mg, 2.12 mmol). The reaction mixture was heated to 80 0C for 24 hours, after which it was cooled down and partitioned between water and ethyl acetate. The aqueous layer was extracted with ethyl acetate (2 x 50 mL) and the combined organic layers were washed with brine, dried over Na2SO4 and concentrated in vacuo. Purification by preparative HPLC, followed by concentration in vacuo and lyophilization afforded the title compound (4.8 mg, 4%) as a white solid. 1H NMR (400 MHz, de-DMSO): 8.21 (m, IH), 7.83 (m, 2H), 7.33 (m, 2H), 7.07 (m, 2H), 7.00 (m, IH), 5.09 (s, 2H); MS (EI) for Ci7HnBrN2O3: 371 (MH+).
(Compound 102)
8-bromo-2-[( { [4-(4-methylpiperazin- 1 - yl)phenyl]methyl| oxy)methyl] [ 1 ]benzofuro[3 ,2-dlpyrimidin-4(3H)-one
[00346] 8-bromo-2-[( { [4-(4-methylpiperazin- 1 - yl)phenyl]methyl}oxy)methyl][l]benzofuro[3,2-d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 3, Compound 15, wherein phenol was substituted with [4-(4-methylpiperazine-lyl) phenyl] methanol. Purification by preparative HPLC afforded the title compound (5 mg, 3%) as a white solid. 1H NMR (400 MHz, d6-DMSO): 8.01 (s, IH), 7.67 (m, 2H), 7.23 (d, 2H), 7.00 (d, 2H), 5.10 (t, IH), 4.53 (s, 2H), 4.39 (d, 2H), 3.93 (m, 2H), 3.75 (m, 2H), 3.52 (m, 2H), 3.29 (s, 3H); MS (EI) for C23H23BrN4O3: 483:485 (Bromine isoptope MH+).
Scheme 2
/ cat H2O
Figure imgf000179_0001
Figure imgf000179_0002
Figure imgf000179_0003
CICHpCOCl
Figure imgf000179_0004
wherein R45 is described within the compounds within this example. EXAMPLE 4: (Scheme 2) 4-Bromo-2-cyanophenyl tert-butyi carbonate 8
[00347] A 250-mL round bottom flask was charged with 4-bromo-2-cyanophenol (3.29 g, 16.64 mmol), ditert-butyl dicarbonate (3.99 g, 18.28 mmol), and DMAP (101 mg, 0.83 mmol). n-Hexane (50 mL), dichloromethane (10 mL) and acetonitrile (10 mL) were added at room temperature and under a positive pressure of nitrogen. The mixture was briefly heated at 50 0C until all material went into solution, then stirred for another 20 min at room temperature. The reaction was judged to have reached completion by TLC. The mixture was diluted with EtOAc (150 mL), washed with 1 N NaHSO4 (20 mL), water (20 mL) and brine (10 mL). The organic layer was dried over MgSO4. After purification by flash chromatography (9:1 hexane/EtOAc), the title compound (8) was obtained as colorless oil (4.89 g), 99% yield. 1H NMR (400 MHz, CDCl3) δ 7.78 (d, J= 2.5 Hz, IH), 7.72 (dd, J= 2.5, J= 11 Hz, IH), 7.24 (d, J= 9 Hz, IH), 1.58 (s, 9H); 13C NMR (100 MHz, CDCl3) δ 151.9, 150.3, 137.4, 135.8, 124.7, 119.1, 113.9, 109.2, 85.9, 53.7. MS (ESI+) for Ci2Hi2BrNO3: 299 (MH+). tert-Butyi 2-cyano-4-cyclopropylphenyl carbonate 9 [00348] A mixture of 4-bromo-2-cyanophenyl tert-butyi carbonate (8, 1.366 g, 4.58 mmol), cyclopropyl boronic acid (531 mg, 6.18 mmol), [Pd(OAc)2] (165 mg, 0.245 mmol), tricyclohexylphosphine (148 mg, 0.528 mmol), and K3PO4 (3.86 g, 18.2 mmol) in toluene (10 mL) and water (1 mL) was heated at 110 0C for 3 h. The mixture was cooled to room temperature and poured onto water, extracted twice with ethyl acetate. The combined organic layers were washed with water and brine, and dried over MgSO4. After purification by flash chromatography (85:15 hexane/EtOAc), the title compound (9) was obtained as yellowish oil (671 mg), 56% yield. 1H NMR (400 MHz, CDCl3) δ 7.33 (d, J= 2.5 Hz, IH), 7.29 (dd, J= 2.5, J= 8 Hz, IH), 7.20 (d, J= 8 Hz, IH), 1.91 (m, IH), 1.58 (s, 9H), 1.03 (m, 2H), 0.70 (m, 2H); 13C NMR (IOO MHz, CDCl3) δ 151.1, 150.4, 143.0, 131.7, 130.5, 122.8, 115.4, 107.1, 85.2, 27.8, 15.0, 9.8; MS (ESI+) for Ci5Hi7NO3: 260 (MH+). 5 -Cvclopropyl-2-hvdroxybenzonitrile 10
[00349] A mixture of tert-butyl 2-cyano-4-cyclopropylphenyl carbonate (9, 671 mg, 2.59 mmol) in dichloromethane (20 mL), trifluoroacetic acid (4 mL) and triethylsilane (2.5 mL) was stirred at room temperature for 16 h. Volatiles were removed in vacuo and replaced with same volume of fresh solvent and reagents. The mixture was heated at 45 0C for 1 h, then concentrated under reduced pressure. After purification by flash chromatography (8:2 hexane/EtOAc), the title compound (10) was obtained as yellowish oil (280 mg), 68% yield, along with N-tert-buty{-5- cyclopropyl-2-hydroxybenzamide (11, 119 mg) as side-product, 20% yield.
1H NMR (400 MHz, CDCl3) δ 7.17 (m, IH), 7.14 (m, IH), 6.94 (d, J= 9 Hz, IH), 1.82 (m, IH), 0.93 (m, 2H), 0.60 (m, 2H); 13C NMR (100 MHz, CDCl3) δ 157.4, 136.4, 132.8, 130.2, 116.7, 99.2, 21.4, 14.6; MS (ESI+) for Ci0H9NO: 160 (MH+). 2-(2-Cyano-4-cyclopropylphenoxy)acetamide 12
[00350] A 100-mL round bottom flask was charged with 5-cyclopropyl-2- hydroxybenzonitrile (11, 280 mg, 1.76 mmol), bromoacetamide (263 mg, 1.91 mmol), potassium carbonate (545 mg, 3.94 mmol) and acetone (20 mL). The mixture was heated at 65 0C for 18 h under nitrogen. The reaction was judged to have reached completion by LC-MS. The mixture was diluted with EtOAc (150 mL), washed with water (20 mL) and brine (10 mL). The organic layer was dried over MgSO4. After removal of solvents under reduced pressure, the title compound (12) was obtained as a white solid (366 mg), 96% yield, which was deemed pure enough for the following treatment.
1H NMR (400 MHz, CDCl3) δ 7.31 (m, 2H), 6.91 (m, IH), 4.55 (s, 2H), 3.64 (br s, 2H), 1.89 (m, IH), 1.01 (m, 2H), 0.66 (m, 2H); 13C NMR (100 MHz, CDCl3) δ 170.6, 156.9, 138.7, 132.7, 131.0, 116.5, 112.7, 101.8, 67.5, 14.5, 9.1; MS (ESI+) for 3-Amino-5-cvclopropylbenzofuran-2-carboxamide 13
[00351] To a solution of 2-(2-cyano-4-cyclopropylphenoxy)acetamide (12, 366 mg, 1.69 mmol) in ethanol (15 mL), a solution of KOH (322 mg, 5.74 mmol) in ethanol (10 mL) was added at 80 0C. Reaction was judged complete (TLC, LC-MS) after 1 h. The mixture was cooled to room temperature and diluted with ethyl acetate (100 mL), then washed with water (30 mL), IM pH=8 phosphate buffer. The combined aqueous layers were extracted again with ethyl acetate (50 mL) and chloroform (2x 40 mL). The combined organic layers were washed with brine (2x), and dried over MgSO4. After purification by flash chromatography (96:4 dichloromethane/methanol), the title compound (13) was obtained as a solid (324 mg), 91% yield.
1H NMR (400 MHz, CDCl3) δ 7.32 (s, IH), 7.24 (d, J= 8 Hz, IH), 7.17 (dd, J= 8, J= 2 Hz, IH), 6.4 (br s, 2H), 5.25 (br s, 2H), 2.00 (m, IH), 0.99 (m, 2H), 0.70 (m, 2H); 13C NMR (IOO MHZ, CDCl3) δ 164.4, 151.7, 138.1, 136.6, 126.9, 122.3, 116.4, 11.4, 15.1, 8.8; MS (ESI+) for Ci2Hi2N2O2: 217 (MH+). 3-(2-Chloroacetamido)-5-cvclopropylbenzofuran-2-carboxamide 14
[00352] A solution of 3-amino-5-cyclopropylbenzofuran-2-carboxamide 13 (183 mg, 0.846 mmol) in chloroacetyl chloride (3 mL) was heated to 40 0C for 1 h. The reaction mixture was quenched with saturated aqueous NaHCO3 (60 mL) and extracted with chloroform (3 x 50 mL). The combined organic phases were washed with water, dried over MgSO4 and concentrated in vacuo to afford the title compound (14) as a white solid (308 mg), contaminated by chloroacetic acid. 1H NMR (400 MHz, CDCl3, spiked with CD3OD) δ 8.10 (s, IH), 7.32 (m, IH), 7.20 (dd, J= 8, J= 2 Hz, IH), 6.8 (br s, IH), 4.09 (s, 2H), 2.03 (m, IH), 0.99 (m, 2H), 0.74 (m, 2H); 13C NMR (100 MHz, CDCl3 spiked with CD3OD) δ 169.8, 164.8, 152.3, 139.5, 127.3, 123.2, 121.6, 111.5, 41.3, 15.6, 9.3; MS (ESI+) for Ci4Hi3ClN2O3: 293 (MH+). 2-(Chloromethyl)-8-cyclopropylbenzofuro[3,2-(/|pyrimidin-4(3/-/)-one 15 [00353] A solution of 3 -(2-chloroacetamido)-5-cyclopropylbenzofuran-2- carboxamide 14 (0.846 mmol) in 10 mL of 2N NaOH was heated to 40 0C for 15 min. The reaction mixture was brought to acidic pH with IN NaHSO4 and extracted with ethyl acetate (2 x 50 mL). The combined organic phases were washed with water, brine, and dried over MgSO4. After purification by flash chromatography (97:3 to 94:6 dichloromethane/methanol), the title compound (15) was obtained as a solid (135 mg), 58% yield over two steps.
1H NMR (400 MHz, CDCl3, spiked with CD3OD) δ 7.74 (s, IH), 7.55 (d, J= 8 Hz, IH), 7.39 (dd, J= 8, J= 2 Hz, IH), 4.61 (s, 2H), 2.08 (m, IH), 1.05 (m, 2H), 0.79 (m, 2H); MS (ESI+) for C14H11ClN2O2: 275 (MH+). (Compound 23)
8-Cvclopropyl-2-r(4-methylpiperazin-l-yl)methylirilbenzofuror3,2-άπpyrimidin-
4(3ifl-one [00354] To a solution of 2-(chloromethyl)-8-cyclopropylbenzofuro[3,2-
</]pyrimidin-4(3H)-one 15 (135 mg, 0.49 mmol) in 8 mL anhydrous ethanol was added 4-methylpiperazine (125 mg, 1.25 mmol). The reaction mixture was heated to
80 0C for 16 hours, cooled down and concentrated in vacuo. After purification by flash chromatography (93:6:1 dichloromethane/methanol/28% (w/w) ammonium hydroxide), the title compound was obtained as a solid (140 mg), 84% yield.
1H NMR (400 MHz, CDCl3) δ 7.66 (s, IH), 7.51 (d, J= 8 Hz, IH), 7.36 (dd, J= 8, J= 2 Hz, IH), 3.69 (s, 2H), 2.68 (br s, 4H), 2.53 (br s, 4H), 2.33 (s, 3H), 2.04 (m, IH),
1.03 (m, 2H), 0.77 (m, 2H); 13C NMR (100 MHz, CDCl3) δ 155.9, 155.1, 153.2,
144.7, 140.5, 138.7, 129.1, 122.5, 117.5, 112.6, 60.3, 55.1, 53.5, 46.1, 15.6, 9.7; MS
(ESI+) for Ci9H22N4O2: 339 (MH+). Scheme 3
K2CO3
Figure imgf000183_0002
Figure imgf000183_0001
Figure imgf000183_0003
wherein X, R6, R7 and R15 are described within the compounds within this example.
EXAMPLE 5: (Scheme 3) ferf -Butyl 2-cyano-4-vinylphenyl carbonate 17 [00355] A mixture of 4-bromo-2-cyanophenyl tert-bvXy\ carbonate (8, 878 mg,
2.94 mmol), tributylvinylstannane (1.155 g, 3.64 mmol) and [Pd(PPh3)4] (72 mg,
0.062 mmol) in toluene (25 mL) was heated at 110 0C for 6 h. The mixture was cooled to room temperature and filtered through Celite®. The clear solution was concentrated under reduced pressure. After purification by flash chromatography (85:15 hexane/EtOAc), the title compound (17) was obtained as colorless oil (625 mg), 87% yield.
1H NMR (400 MHz, CDCl3) δ 7.66 (d, J= 2.5 Hz, IH), 7.62 (dd, J= 2.5, J= 8 Hz, IH), 7.28 (d, J= 8 Hz, IH), 6.66 (dd, J= 11, J= 17 Hz, IH ), 5.76 (d, J= 11 Hz, IH), 5.37
(d, J= 17 Hz, IH), 1.58 (s, 9H); 13C NMR (100 MHz, CDCl3) δ 151.9, 150.8, 136.3,
134.2, 131.8, 130.9, 123.2, 116.9, 115.2, 107.6, 85.5, 27.8; MS (ESI+) for
Figure imgf000183_0004
5 -( 1 -ChloroethvD-2-hvdroxybenzonitrile 18 [00356] A mixture of tert-butyi 2-cyano-4-vinylphenyl carbonate (17, 597 mg,
2.44 mmol) was treated with 4 N HCl in dioxane (6 mL) at 50 0C for 90 min. The reaction mixture was quenched with saturated aqueous NaHCO3 (40 mL) and extracted with EtOAc (2 x 50 mL). The combined organic phases were washed with water and brine, dried over MgSO4. After purification by flash chromatography (8:2 hexane/EtOAc), the title compound (18) was obtained as yellowish oil (301 mg), 68% yield. 1H NMR (400 MHz, CD3OD) δ 7.48 (d, J= 2.5 Hz, IH), 7.45 (dd, J= 2.5, J= 8 Hz, IH), 6.91 (d, J= 8 Hz, IH), 4.75 (q, J= 6 Hz, IH ), 1.39 (d, J= 6 Hz, 3H); 13C NMR (100 MHz, CD3OD) δ 159.5, 138.1, 132.1, 130.0, 116.9, 115.9, 99.0, 68.5, 24.2; MS (ESI+) for C9H8ClNO: 182 (MH+). 2-(4-(l-Chloroethyl)-2-cyanophenoxy)acetamide 19 [00357] A 100-mL round bottom flask was charged with 5-(l-chloroethyl)-2- hydroxybenzonitrile (18, 301 mg, 1.66 mmol), bromoacetamide (252 mg, 1.83 mmol), potassium carbonate (486 mg, 3.52 mmol) and acetone (20 mL). The mixture was heated at 65 0C for 24 h under nitrogen. The reaction was judged to have reached completion by LC-MS. The mixture was diluted with EtOAc (150 mL), washed with water (20 mL) and brine (10 mL). The organic layer was dried over MgSO4. After removal of solvents under reduced pressure, the title compound (19) was obtained as a white solid (322 mg), 81% yield, which was deemed pure enough for the following treatment. 1H NMR (400 MHz, CD3OD) δ 7.65 (m, IH), 7.62 (m, IH), 7.08 (d, J= 8 Hz, IH), 4.81 (q, J= 6 Hz, IH ), 4.67 (s, 2H), 1.40 (d, J= 6 Hz, 3H); 13C NMR (100 MHz, CD3OD) δ 171.5, 158.7, 140.8, 132.0, 130.6, 116.1, 112.8, 101.6, 68.2, 67.4, 24.3; MS (ESI+) for C11H11CIN2O2: 239 (MH+). 3 - Amino-5 -( 1 -hydroxy ethyl)benzofuran-2-carboxamide 20 [00358] To a solution of 2-(4-(l-chloroethyl)-2-cyanophenoxy)acetamide (19, 322 mg, 1.35 mmol) in ethanol (40 mL), a solution of KOH (223 mg, 3.97 mmol) in ethanol (20 mL) was added at 50 0C. The mixture was heated at 75 0C. Reaction was judged complete (TLC, LC-MS) after 1.5 h. The mixture was cooled to room temperature and diluted with ethyl acetate (100 mL), then washed with water (30 mL), IM pH=8 phosphate buffer. The combined aqueous layers were extracted again with ethyl acetate (50 mL) and chloroform (2x 40 mL). The combined organic layers were washed with brine (2x), and dried over MgSO4. After purification by flash chromatography (96:4 dichloromethane/methanol), the title compound (20) was obtained as a solid (324 mg), quantitative yield. 1H NMR (400 MHz, CD3OΔ) δ 7.74 (m, IH), 7.47 (m, IH), 7.35 (d, J= 8 Hz, IH), 4.93 (q, J= 6 Hz, IH ), 1.48 (d, J= 6 Hz, 3H); MS (ESI+) for CnHi2N2O3: 221 (MH+). 2-(Chloromethyl)-8-(l-hydroxyethyl)benzofuro[3,2-άπpyrimidin-4(3H)-one 21 [00359] A solution of 3-amino-5-(l-hydroxyethyl)benzofuran-2-carboxamide (20, 324 mg, 1.47 mmol) in chloroacetyl chloride (3 rnL) was heated to 40 0C for 1 h. The reaction mixture was quenched and vigorously stirred for 15 min with saturated aqueous NaHCO3 (60 mL), extracted with EtOAc (2 x 50 mL) and chloroform (2 x 50 mL). The combined organic phases were washed with water, dried over MgSO4 and concentrated in vacuo to afford the intermediate, l-(2-carbamoyl-3-(2- chloroacetamido)benzofuran-5-yl)ethyl 2-chloroacetate, as a white solid (1.005 g), contaminated by chloroacetic acid. A solution of this material, which did not undergo further purification, in 8 mL of 2 N NaOH was heated to 40 0C for 10 min, then stirred at room temperature for another 20 min. The reaction mixture was brought to acidic pH with IN NaHSO4 and extracted with ethyl acetate (2 x 50 mL) and dichloromethane (50 mL). The combined organic phases were washed with water, brine, and dried over MgSO4. After purification by flash chromatography (97:3 to 94:6 dichloromethane/methanol), the title compound (21) was obtained as a solid (197 mg), 48% yield over two steps. 1H NMR (400 MHz, 3:1 CD3OD/CDCI3) δ 8.07 (m, IH), 7.69 (m, IH), 7.64 (d, J= 8 Hz, IH), 5.02 (q, J= 6 Hz, IH ), 4.63 (s, 2H), 1.53 (d, J= 6 Hz, 3H); 13C NMR (100 MHz, 3:1 CD3OD/CDCI3) δ 156.6, 153.8, 143.4, 138.9, 128.3, 122.0, 118.0, 112.6, 69.4, 42.2, 24.9; MS (ESI+) for Ci3Hi 1ClN2O3: 279 (MH+).
(Compound 27) 8-( 1 -Hydroxyethyl)-2- [(4-methylpiperazin- 1 -yDmethyl) [ 1 Ibenzofuro 3 ,2-<i]pyrimidin-4(3/-/)-one [00360] To a solution of 2-(chloromethyl)-8-(l-hydroxyethyl)benzofuro[3,2- </]pyrimidin-4(3H)-one (21, 197 mg, 0.70 mmol) in 15 mL anhydrous ethanol was added 4-methylpiperazine (192 mg, 1.92 mmol). The reaction mixture was heated to 80 0C for 16 hours, cooled down and concentrated in vacuo. After purification by flash chromatography (90:8.5:1.5 dichloromethane/methanol/28% (w/w) ammonium hydroxide), the title compound was obtained as a solid (205 mg), 86% yield.
1H NMR (400 MHz, 3:1 CD3OD/CDC13) δ 8.06 (m, IH), 7.67 (dd, J= 2.5, J= 8 Hz, IH), 7.62 (d, J= 8 Hz, IH), 5.02 (q, J= 6 Hz, IH ), 3.68 (s, 2H), 2.69 (br, 4H), 2.59 (br, 4H), 2.33 (s, 3H), 1.53 (d, J= 6 Hz, 3H); 13C NMR (100 MHz, 3:1 CD3OD/CDCI3) δ 156.6, 156.1, 154.5, 144.5, 143.2, 138.7, 128.1, 122.1, 118.1, 112.5, 69.4, 60.4, 54.6, 52.6, 45.1, 25.0; MS (ESI+) for Ci8H22N4O3: 343 (MH+).
(Compound 29)
8-Acetyl-2-[(4-methylpiperazin- 1 -yDmethyl] [ 1 ]benzofuro[3 ,2-</|pyrimidin-4(3/-f)-one
[00361] To a solution of Compound 27 (54 mg, 0.158 mmol) in 5 rnL anhydrous dichloromethane was added anhydrous NMP (0.2 mL) and Dess-Martin periodinane (120 mg, 0.28 mmol). The reaction mixture was stirred at room temperature for 2 hours. The reaction mixture was quenched with saturated aqueous NaHCO3 (10 mL) and extracted with EtOAc (2x 40 mL) and chloroform (2 x 30 mL). The combined organic phases were washed with water, dried over MgSO4. After purification by flash chromatography (88:10:2 dichloromethane/methanol/28% (w/w) ammonium hydroxide), the title compound was obtained as a solid (56 mg), quantitative yield. 1H NMR (400 MHz, 3:1 CD3OD/CDC13) δ 8.77 (m, IH), 8.30 (dd, J= 2.5, J= 8 Hz, IH), 7.81 (m, IH), 3.72 (s, 2H), 2.75 (br, 8H), 2.74 (s, 3H), 2.47 (s, 3H); MS (ESI+) for Ci8H20N4O3: 341 (MH+).
Scheme 4
Figure imgf000186_0001
wherein R3a is as defined in the disclosure above and R15 is described within the compounds within this example.
EXAMPLE 6: (Scheme 4)
5-bromo-3-ureidobenzofuran-2-carboxamide 24
[00362] To a suspension of 3-amino-5-bromobenzofuran-2-carboxamide (1.0 g,
3.95 mmol) in 40 mL acetic acid and 20 mL water was added sodium cyanate (1.1 g, 18.6 mmol). The reaction was stirred for 48 hours at room temperature, diluted with water (100 mL) and extracted with ethyl acetate (2 x 150 mL). The combined organic phases were washed with brine, dried over Na2SO4 and concentrated in vacuo. The product was purified by SiO2 flash chromatography (20:80 hexanes/ethyl acetate to 20:80 methanol/ethyl acetate to afford 5-bromo-3-ureidobenzofuran-2-carboxamide (0.7 g, 60%) as a white solid. 1H NMR (400 MHz, d6-DMSO): 9.03 (s, IH), 8.37 (d, IH), 8.05 (br s, IH), 7.79 (br s, IH), 7.60 (dd, IH), 7.5 (d, IH), 6.7 (br s, 2H) ); MS (EI) for Ci0H8BrN3O3: 298.1 (MH+).
8-bromobenzofuro[3,2-dlpyrimidine-2,4(lH,3H)-dione 25
[00363] A suspension of 5-bromo-3-ureidobenzofuran-2-carboxamide (24) (0.7 g, 2.35 mmol) in 20 mL aqueous 2N NaOH was heated to 95 0C for 2 hours. The reaction mixture was cooled to room temperature and acidified with IN HCl (50 mL). The precipitate was washed with a large quantity of water (500 mL) and dried to afford 8-bromobenzofuro[3,2-d]pyrimidine-2,4(lH,3H)-dione (0.5 g, 76%) as a white solid. 1H NMR (400 MHz, d6-DMSO): 8.15 (m, IH), 7.76 (m, 2H); MS (EI) for Ci0H5BrN2O3: 281.1 (MH+). 8-bromo-2,4-dichlorobenzofuror3,2-dlpyrimidine 26 [00364] A suspension of 8-bromo-2,4-dichlorobenzofuro[3,2-d]pyrimidine (25) (0.820 g, 2.35 mmol) in 2 mL of phenylphosphonic dichloride was heated to 160 0C for 2 hours. After cooling down to room temperature, the reaction was poured on ice- water and extracted with ethyl acetate (2 x 150 mL). The combined organic phases were washed with brine, dried over Na2SO4 and concentrated in vacuo. The product was purified by SiO2 flash chromatography (90:10 hexanes/ethyl acetate) to afford 8- bromo-2,4-dichlorobenzofuro[3,2-d]pyrimidine (0.372 g, 48%) as a yellow solid. 1H NMR (400 MHz, d6-DMSO): 8.54 (m, IH), 8.05 (m, 2H). 8-bromo-2-chlorobenzofuro[3,2-d]pyrimidin-4(3H)-one 27 [00365] To a solution of 8-bromo-2,4-dichlorobenzofuro[3,2-d]pyrimidine (26) (0.372 g, 1.12 mmol) in 5 mL dioxane was added 2N NaOH (2 mL) and heated to 85 0C for 10 minutes. The reaction mixture was cooled to room temperature, acidified with IN HCl (10 mL) and extracted with ethyl acetate (2 x 100 mL). The combined organic phases were washed with brine, dried over Na2SO4 and concentrated in vacuo to afford 8-bromo-2-chlorobenzofuro[3,2-d]pyrimidin-4(3H)-one (0.338 g, quant.). 1H NMR (400 MHz, d6-DMSO): 8.24 (m, IH), 7.85 (m, 2H); MS (EI) for Ci0H4BrClN2O2: 297 (M-H).
(Compound 115)
8-bromo-2-(4-methylpiperazin-l-yl)πibenzofuror3,2-dlpyrimidin-4(3H)-one [00366] To a solution of 8-bromo-2-chlorobenzofuro[3,2-d]pyrimidin-4(3H)-one (27) (53 mg, 0.18 mmol) in 2 niL ethanol was added N-methylpiperazine (180 mg, 1.80 mmol) and heated to 80 0C for 14 hours. The reaction mixture was concentrated in vacuo and purified by preparative HPLC. Concentration in vacuo and lyophilization afforded 8-bromo-2-(4-methylpiperazin-l-yl)[l]benzofuro[3,2- d]pyrimidin-4(3H)-one (23.7 mg, 36%). 1H NMR (400 MHz, d6-DMSO): 8.05 (m, IH), 7.72 (m, 2H), 3.65 (m, 4H), 2.40 (m, 4H), 2.22 (s, 3H); MS (EI) for Ci5Hi5BrN4O2: 363 (MH+).
(Compound 180) 8-bromo-2-(phenylamino)rilbenzofuror3,2-dlpyrimidin-4(3H)-one
[00367] 8-bromo-2-(phenylamino)[l]benzofuro[3,2-d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 6, Compound 115, wherein N- methylpiperazine was substituted with aniline. The product was filtered off, washed with cold EtOH and dried in vacuo to afford the title compound (53.4 mg, 75%). 1H NMR (400 MHz, d6-DMSO): 11.25 (s, IH), 8.84 (m, IH), 8.18 (m, IH), 7.76 (m, 4H), 7.39 (m, 2H), 7.08 (m, IH). MS (EI) for Ci6Hi0BrN3O2: 356.1 (MH+).
(Compound 193)
8-bromo-2-{[3-(dimethylamino)propyllamino|[llbenzofuro[3,2-dlpyrimidin-4(3H)- one [00368] 8-bromo-2-{[3-(dimethylamino)propyl]amino} [l]benzofuro[3,2- d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 6, Compound 115, wherein N-methylpiperazine was substituted with N ,N- dimethylpropane-l,3-diamine. Purification by preparative HPLC was followed by concentration in vacuo and lyophilization to afford the title compound as an acetate salt (20.8 mg, 28%). 1H NMR (400 MHz, d6-DMSO): 7.95 (m, IH), 7.67 (m, 2H), 3.33 (m, 2H), 2.29 (t, 2H), 1.84 (s, 3H), 1.67 (m, 2H). MS (EI) for Ci5Hi7BrN4O2: 363 (M-H).
(Compound 196)
8-bromo-2-pyrrolidin- 1 -yl[ 1 lbenzofuro[3 ,2-dlpyrimidin-4(3H)-one [00369] 8-bromo-2-pyrrolidin-l-yl[l]benzofuro[3,2-d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 6, Compound 115, wherein N- methylpiperazine was substituted with pyrrolidine. Purification by preparative HPLC was followed by concentration in vacuo and lyophilization to afford the title compound (7.7 mg, 13.7%). 1H NMR (400 MHz, d6-DMSO): 8.00 (m, IH), 7.70 (m, 2H), 3.52 (m, 4H), 1.91 (m, 4H). MS (EI) for Ci4Hi2BrN3O2: 334 (MH+).
(Compound 213) 8-bromo-2-(piperidin-4-ylamino)πibenzofuror3,2-dlpyrimidin-4(3H)-one
[00370] t-butyl-4-(8-bromo-4-oxo-3,4-dihydrobenzofuro[3,2-d]pyrimidin-2- ylamino)piperidine-l-carboxylate was synthesized in a manner similar to Example 6, Compound 115, wherein N-methylpiperazine was substituted with tert-butyl 4- aminopiperidine-1-carboxylate (HCl salt) and Cs2CO3 was employed as a base. After heating at 100 0C for 48 hours, the reaction mixture was cooled to room temperature, diluted with water and extracted with ethyl acetate (2 x 50 mL). The combined organic phases were washed with brine, dried over Na2SO4 and concentrated in vacuo to afford tert-butyl 4-(8-bromo-4-oxo-3,4-dihydrobenzofuro[3,2-d]pyrimidin-2- ylamino)piperidine-l-carboxylate. To a suspension of the crude material in methanol (4 mL) was added 4N HCl/dioxane (2 mL) and stirred at room temperature for 12 hours. The reaction mixture was concentrated in vacuo and purified by preparative HPLC. Concentration in vacuo and lyophilization afforded the title compound (12 mg, 13%). 1H NMR (400 MHz, d6-DMSO): 7.99 (m, IH), 7.70 (m, 2H), 3.95 (m, IH), 3.08 (m, 2H), 2.72 (m, 2H), 1.97 (m, 2H), 1.46 (m, 2H). MS (EI) for Ci5Hi5BrN4O2: 363 (MH+).
(Compound 214)
8-bromo-2-(pyridin-3-ylamino)[llbenzofuro[3,2-dlpyrimidin-4(3H)-one
[00371] 8-bromo-2-(pyridin-3-ylamino)[l]benzofuro[3,2-d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 6, Compound 115, wherein N- methylpiperazine was substituted with pyridin-3-amine. The product was filtered off and dried in vacuo to afford the title compound (33 mg, 40%).
1H NMR (400 MHz, d6-DMSO): 9.35 (s, IH), 9.15 (m, IH), 8.11 (s, IH), 7.82 (m, IH), 7.74 (m, 3H), 6.77 (s, IH). MS (EI) for Ci5H9BrN4O2: 357.1 (MH+).
(Compound 225) 8-bromo-2-( { [4-(4-methylpiperazin- 1 -yl)phenyl]methyl| amino) [ 1 ]benzofuro [3,2- d"|pyrimidin-4(3H)-one [00372] 8-bromo-2-({[4-(4-methylpiperazin-l- yl)phenyl]methyl}amino)[l]benzofuro[3,2-d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 6, Compound 115, wherein N-methylpiperazine was substituted with (4-(4-methylpiperazin-l-yl)phenyl)methanamine and CS2CO3 was used as a base. The reaction mixture was concentrated in vacuo and purified by preparative HPLC. Concentration in vacuo and lyophilization afforded the title compound (12.4 mg, 13%). 1H NMR (400 MHz, d6-DMSO): 8.02 (m, IH), 7.73 (m, 2H), 7.33 (m, 2H), 6.99 (m, 2H), 4.9 (m, 2H), 3.82 (m, 2H), 3.52 (m, 2H, overlapped), 3.38 (s, 3H, overlapped), 2.85 (m, 4H). MS (EI) for C22H22BrN5O2: 467.9 (MH+). (Compound 227)
8-bromo-2- {4-[2-(dimethylamino)ethyl"|piperazin- 1 -yl| [ 1 lbenzofuro|"3,2-d]pyrimidin- 4(3H)-one
[00373] 8-bromo-2- {4-[2-(dimethylamino)ethyl]piperazin- 1 -yl} [ 1 ]benzofuro[3,2- d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 6, Compound 115, wherein N-methylpiperazine was substituted with N,N-dimethyl-2- (piperazin-l-yl)ethanamine. The reaction mixture was concentrated in vacuo and purified by preparative HPLC. Concentration in vacuo and lyophilization afforded the title compound (23.6 mg, 24 %). 1H NMR (400 MHz, d6-DMSO): 8.02 (m, IH), 7.69 (m, 2H), 3.62 (m, 4H), 2.49 (m, 4H), 2.44 (m, 4H), 2.2 (s, 6H). MS (EI) for Ci8H22BrN5O2: 420 (MH+).
(Compound 228)
8-bromo-2-[4-( 1 -methylpiperidin-4-yl)piperazin- 1 -yl] [ 1 ]benzofuro [3 ,2-d]pyrimidin- 4(3H)-one
[00374] 8-bromo-2-[4-(l -methylpiperidin-4-yl)piperazin- 1 -yl] [ 1 ]benzofuro[3 ,2- d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 6, Compound 115, wherein N-methylpiperazine was substituted with 1-(1- methylpiperidin-4-yl)piperazine. The reaction mixture was concentrated in vacuo and purified by preparative HPLC. Concentration in vacuo and lyophilization afforded the title compound (57 mg, 60%). 1H NMR (400 MHz, d6-DMSO): 8.03 (m, IH), 7.71 (m, 2H), 3.63 (m, 4H), 2.83 (m, 2H), 2.55 (m, 4H), 2.51 (m, 2H, overlapped), 2.16 (s, 3H), 1.87 (m, IH), 1.74 (m, 2H), 1.42 (m, 2H). MS (EI) for C20H24BrN5O2: 445.9 (MH+). (Compound 245)
8-bromo-2-[4-(N,N-diethylglycyl)piperazin- 1 -yl] [ 1 ]benzofuro[3 ,2-d]pyrimidin- 4(3HVone
[00375] 8-bromo-2-[4-(N,N-diethylglycyl)piperazin-l-yl][l]benzofuro[3,2- d]pyrimidin-4(3H)-one 8-bromo-2-[4-(l-methylpiperidin-4-yl)piperazin-l- yl][l]benzofuro[3,2-d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 6, Compound 115, wherein N-methylpiperazine was substituted with 2- (diethylamino)-l-(piperazin-l-yl)ethanone. The reaction mixture was concentrated in vacuo and purified by preparative HPLC reverse-phase, 0.1% TFA in acetonitrile/0.05% TFA in water). Concentration in vacuo and lyophilization afforded the title compound (15.3 mg, 16%). 1H NMR (400 MHz, d6-DMSO): 8.06 (m, IH), 7.76 (m, 2H), 4.30 (m, 2H), 3.75 (m, 4H), 3.66 (m, 2H), 3.54 (m, 2H), 3.14 (m, 4H), 1.22 (t, 6H). MS (EI) for C20H24BrN5O3: 461.9 (MH+).
(Compound 246)
8-bromo-2-{4-r3-(dimethylamino)propyllpiperazin-l-vUrilbenzofuror3,2- dlpyrimidin-4(3H)-one
[00376] 8-bromo-2-{4-[3-(dimethylamino)propyl]piperazin-l-yl}[l]benzofuro[3,2- d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 6, Compound 115, wherein N-methylpiperazine was substituted with N,N-dimethyl-3- (piperazin-l-yl)propan-l -amine. The reaction mixture was concentrated in vacuo and purified by preparative HPLC.
Concentration in vacuo and lyophilization afforded the title compound (23 mg, 24%). 1H NMR (400 MHz, d6-DMSO): 8.02 (m, IH), 7.70 (m, 2H), 3.63 (m, 4H), 2.44 (m, 4H), 2.32 (m, 4H), 2.20 (s, 6H), 1.61 (m, 2H). MS (EI) for Ci9H24BrN5O2: 433.9 (MH+).
Scheme 5
Figure imgf000191_0001
wherein R3a is as defined in the disclosure above and Ri6 is hydrogen or piperidin-4- ylmethyl)amino]pyrrolidin- 1 -yl. EXAMPLE 7: (Scheme 5)
(Compound 28)
2-[(3-aminopyrrolidin- 1 -yl)methyl"|-8-bromo[ 11benzofuro|"3,2-d]pyrimidin-4(3H)-one (HCl salt) [00377] To a solution of 8-bromo-2-(chloromethyl)benzofuro[3,2-d]pyrimidin- 4(3H)-one 6 (300 mg, 0.96 mmol) in 2.0 mL anhydrous DMF was added 3-(tert- butoxy carbonyl amino)pyrrolidine (178 mg, 0.96 mmol) and CS2CO3 (311 mg, 0.96 mmol). The reaction mixture was heated to 80 0C at 150 W for 10 minutes in a CEM- Discover microwave reactor. The reaction mixture was filtered and concentrated in vacuo. Purification by flash chromatography (60:40 ethyl acetate :hexanes to 100% ethyl acetate afforded the Boc protected title compound as a solid. The pure intermediate was then dissolved in MeOH (3.OmL) and 4M HCl in Dioxanes (2.0 mL) and stirred at room temperature for 6 h. The resulting precipitate was filtered and rinsed with MeOH 3X to afforded the title compound (163 mg, 47%) as a light tan solid. 1H NMR (400 MHz, d6-DMSO+D2O): 8.32 (s, IH), 7.79 (m, 2H), 4.40 (m, 3H), 4.00 (m, IH), 3.60 (m, 4H), 3.19 (m, IH), 2.48 (m, IH), 2.09 (m, IH); MS (EI) for Ci5Hi5BrN4O2: 363 (MH+).
(Compound 110)
8-bromo-2-( {3-r(piperidin-4-ylmethyl)aminolpyrrolidin- 1 - yl|methyl)[llbenzofuro[3,2-dlpyrimidin-4(3H)-one
[00378] To a solution of Compound 28 (30 mg, 0.083 mmol) in 2.0 mL anhydrous 1,2-DCE was added 4-formyl-piperidine-l-carboxylic acid t-butyl ester (19 mg, 0.091 mmol) and acetic acid (0.1 mL). The reaction mixture was stirred at ambient temp. Before adding sodium triacetoxy borohydride (52 mg, 0.249 mmol). After overnight stirring at room temperature, an additional 1.0 eq of sodium triacetoxy borohydride was added and stirred until the reaction went to completion. The reaction was quenched with EtOAc/ H2O, partitioned, dried with Na2SO4, concentrated and purified via flash chromatography to afford the Boc protected title compound as a solid. The pure intermediate was then dissolved in MeOH (1.OmL) and 4M HCl in Dioxanes (0.7 mL) and stirred at room temperature for 2 h. The reaction was then concentrated, dissolved in H2O and lyophilized 4X to afforded the title compound (40mg, 90%). 1H NMR (400 MHz, d6-DMSO+D2O): 8.34 (s, IH), 7.87 (m, 2H), 4.55 (br s, 2H), 3.25 (m, 4H), 2.83 (m, 3H), 1.79 (m, 5H), 1.29 (m, 3H); MS (EI) for C2IH26BrN5O2: 460:462 (Bromine isotope, MH+). EXAMPLE 8
Figure imgf000193_0001
wherein R3a is as defined in the disclosure above and R5 is described within the compounds within this example.
(Compound 30)
8-Bromo-2-(2-chlorophenyl)πibenzofuror3,2-dlpyrimidin-4(3H)-one [00379] A solution of 3-amino-5-bromobenzofuran-2-carboxamide 3 (200 mg, 0.78 mmol) and 2-chlorobenzaldehyde (175 μL, 1.55 mmol) in 3 mL anhydrous ethanol was heated to 80 0C. Concentrated hydrochloric acid (20 μL) was added and a precipitate formed immediately. The precipitate was filtered, washed with 1 mL ethanol and air dried to give 190 mg of imine. The precipitate was suspended in 3 mL of dimethylacetamide and heated to 150 0C for 2 hr. Sodium bisulfite (80 mg, 0.76 mmol) was added and the reaction was heated at 150 0C for another 45 min. After cooling, the reaction mixture was filtered and the filtrate was diluted with 5 mL of water. The precipitate was filtered, washed with 1 mL of methanol and dried under vacuum to give 19 mg of 8-bromo-2-(2-chlorophenyl)[l]benzofuro[3,2-d]pyrimidin- 4(3H)-one. 1H NMR (400 MHz, d6-DMSO): 13.34 (m, IH), 8.19 (d, IH), 7.82 (d, IH), 7.79 (dd, IH), 7.63 (dd, IH), 7.59 (dd, IH), 7.54 (m, IH), 7.46 (m, IH); MS (EI) for Ci6H8BrClN2O2: 375 (MH+).
(Compound 36)
8-Bromo-2-(2,6-dichlorophenyl)[llbenzofuro[3,2-dlpyrimidin-4(3H)-one [00380] 8-Bromo-2-(2,6-dichlorophenyl)[l]benzofuro[3,2-d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 8, wherein 2-chlorobenzaldehyde was substituted with 2,6-dichlorobenzaldehyde. 1H-NMR (400MHz, d6-DMSO): 13.64 (s, IH), 8.29 (s br, IH), 7.88 (m, 2H), 7.67 (m, 3H). MS (EI) for Ci6H7BrCl2N2O2: 410.8 (MH+). (Compound 37)
8-Bromo-2-(2,5-dichlorophenyl)[llbenzofuro[3,2-dlpyrimidin-4(3H)-one [00381] 8-Bromo-2-(2,5-dichlorophenyl)[l]benzoflιro[3,2-d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 8, wherein 2-chlorobenzaldehyde was substituted with 2,5-dichlorobenzaldehyde. 1H-NMR (400MHz, d6-DMSO): 13.47 (s, IH), 8.25 (d, IH), 7.87 (s, IH), 7.85 (m, 2H), 7.68 (d, 2H). MS (EI) for Ci6H7BrCl2N2O2: 410.7 (MH+).
(Compound 38)
8-Bromo-2-(2-bromophenyl)rilbenzofuror3,2-dlpyrimidin-4(3H)-one [00382] 8-Bromo-2-(2-bromophenyl)[l]benzofuro[3,2-d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 8, wherein 2-chlorobenzaldehyde was substituted with 2-bromobenzaldehyde. 1H NMR (400 MHz, J6-DMSO): 13.38 (br s, IH), 8.23 (d, IH), 7.85 (s, IH), 7.84 (d, IH), 7.81 (d, IH), 7.65 (dd, IH), 7.54 (td, IH), 7.50 (td, IH); MS (EI) for Ci6H8Br2N2O2: 421 (MH+). (Compound 39)
8-bromo-2-(2-chloro-6-fluorophenyl)rilbenzofuror3,2-dlpyrimidin-4(3H)-one [00383] 8-bromo-2-(2-chloro-6-fluorophenyl)[l]benzofuro[3,2-d]pyrimidin-4(3H)- one was synthesized in a manner similar to Example 8, wherein 2- chlorobenzaldehyde was substituted with 2-chloro-6-fluorobenzaldehyde. 1H NMR (400 MHz, J6-DMSO): 13.32 (br s, IH), 8.25 (d, IH), 7.87 (s, IH), 7.86 (d, IH), 7.81 (td, IH), 7.65 (m, IH), 7.41(q, 2H); MS (EI) for Ci6H7BrCl FN2O2: 394 (MH+).
(Compound 40)
8-bromo-2-(2-iodophenyl)[llbenzofuro[3,2-dlpyrimidin-4(3H)-one [00384] 8-bromo-2-(2-iodophenyl)[l]benzofuro[3,2-d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 8, wherein 2-chlorobenzaldehyde was substituted with 2-iodobenzaldehyde. 1H NMR (400 MHz, d6-DMSO): 13.35 (s, IH), 8.26 (d, IH), 8.01 (d, IH), 7.87 (m, 2H), 7.59 (m, 2H), 7.32 (m, IH); MS (EI) for Ci6H8BrIN2O2: 467 (MH+).
(Compound 41) 8-bromo-2-[2-chloro-4-(dimethylamino)phenyll[llbenzofuro[3,2-dlpyrimidin-4(3H)- one [00385] 8-bromo-2-[2-chloro-4-(dimethylamino)phenyl][l]benzofuro[3,2- d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 8, wherein 2- chlorobenzaldehyde was substituted with 2-chloro-4-(dimethylamino)benzaldehyde. 1H NMR (400 MHz, d6-DMSO): 13.05 (s, IH), 8.20 (d, IH), 7.82 (m, 2H), 7.46 (d, IH), 6.77 (m, 2H), 2.85 (s, 6H); MS (EI) for Ci8Hi3BrClN3O2: 420 (MH+).
(Compound 42)
8-bromo-2-(2-chloro-4-fluorophenyl)[llbenzofuro[3,2-dlpyrimidin-4(3H)-one [00386] 8-bromo-2-(2-chloro-4-fiuorophenyl)[l]benzofuro[3,2-d]pyrimidin-4(3H)- one was synthesized in a manner similar to Example 8, wherein 2- chlorobenzaldehyde was substituted with 2-chloro-4-fluorobenzaldehyde.
1H NMR (400 MHz, d6-DMSO): 13.45 (s, IH), 8.25 (d, IH), 7.86 (m, 2H), 7.77 (m, IH), 7.68 (m, IH), 7.43 (m, IH); MS (EI) for Ci6H7BrClN2O2: 395 (MH+).
(Compound 43)
8-bromo-2-(3-bromopyridin-4-yl)[llbenzofuro[3,2-dlpyrimidin-4(3H)-one [00387] 8-bromo-2-(3-bromopyridin-4-yl)[ 1 ]benzofuro[3,2-d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 8, wherein 2-chlorobenzaldehyde was substituted with 3-bromo-4-pyridinecarboxaldehyde.
1H NMR (400 MHz, d6-DMSO): 13.55 (s, IH), 8.96 (s, IH), 7.76 (d, IH), 8.27 (s, IH), 7.88 (m, 2H), 7.75 (d, IH); MS (EI) for Ci5H7Br2N3O2: 422 (MH+). (Compound 44)
8-bromo-2-(2-chloro-5-fluorophenyl)rilbenzofuror3,2-dlpyrimidin-4(3H)-one [00388] 8-bromo-2-(2-chloro-5-fiuorophenyl)[l]benzofuro[3,2-d]pyrimidin-4(3H)- one was synthesized in a manner similar to Example 8, wherein 2- chlorobenzaldehyde was substituted with 2-chloro-5-fluorobenzaldehyde was substituted. 1H NMR (400 MHz, d6-DMSO): 13.47 (s, IH), 8.26 (d, IH), 7.85-7.90 (m, 3H), 7.69 (d, 2H); MS (EI) for Ci6H7BrClFN2O2: 393 (MH+).
(Compound 52)
8-bromo-2-(2,4-dichlorophenyl)[llbenzofuro[3,2-dlpyrimidin-4(3H)-one [00389] 8-bromo-2-(2,4-dichlorophenyl)[l]benzofuro[3,2-d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 8, wherein 2-chlorobenzaldehyde was substituted with 2,4-dichlorobenzaldehyde. 1H NMR (400 MHz, d6-DMSO): 13.45 (s, IH), 8.24 (s, IH), 7.86 (m, 3H), 7.73 (d, IH), 7.62 (dd, IH); MS (EI) for Ci6H7BrCl2N2O2: 411 (MH+).
(Compound 55)
8-bromo-2-(2,3-dichlorophenyl)[llbenzofuro[3,2-dlpyrimidin-4(3H)-one [00390] 8-bromo-2-(2,4-dichlorophenyl)[l]benzofuro[3,2-d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 8, wherein 2-chlorobenzaldehyde was substituted with 2,3-dichlorobenzaldehyde. 1H NMR (400 MHz, d6-DMSO): 13.47 (s, IH), 8.25 (s, IH), 7.86 (m, 3H), 7.69 (d, IH), 7.57 (t, IH); MS (EI) for Ci6H7BrCl2N2O2: 411 (MH+). (Compound 87)
2-(2-chloro-6-fluorophenyl)-8-cvclopropyiπibenzofuror3,2-dlpyrimidin-4(3H)-one [00391] The title compound was synthesized in a manner similar to Example 8, wherein 3-amino-5-bromobenzofuran-2-carboxamide was substituted with 3-amino-5- cyclopropylbenzofuran-2-carboxamide (whose preparation is described in Example 4) and 2-chlorobenzaldehyde with 2-chloro-6-fluorobenzaldehyde. After purification by flash chromatography (91 :8:1 dichloromethane/methanol/28% (w/w) ammonium hydroxide), the title compound was obtained as a solid (89 mg), 36% yield. 1H NMR (400 MHz, 3:1 CD3OD/CDCI3) δ 7.79 (m, IH), 7.61 (m, 2H), 7.45 (m, 2H), 7.31 (m, IH), 3.32 (m, IH), 2.08 (m, IH), 1.04 (m, 2H), 0.77 (m, 2H); 19F NMR (376 MHz, 3:1 CD3OD/CDCI3) δ -113.5 (dd, J= 5.3, J= 9.4 Hz); MS (ESI+) for Ci9Hi2ClFN2O2: 355 (MH+).
(Compound 64)
8-bromo-2-r2-chloro-3-(methyloxy)phenylirilbenzofuror3,2-dlpyrimidin-4(3H)-one [00392] 8-bromo-2-[2-chloro-3-(methyloxy)phenyl][l]benzofuro[3,2-d]pyrimidin- 4(3H)-one was synthesized in a manner similar to Example 8, wherein 2- chlorobenzaldehyde was substituted with 2-chloro-3-methoxybenzaldehyde. IH NMR (400 MHz, d6-DMSO): 13.39 (s, IH), 8.24 (s, IH), 7.87 (m, 2H), 7.48 (m, IH), 7.36 (d, IH), 7.24 (d, IH), 3.94 (s, 3H). MS (EI) for C17 HlO Br C1 N2 03: 407 (MH+).
(Compound 65) 8-bromo-2-[2-(trifluoromethyl)phenyll[llbenzofuro[3,2-dlpyrimidin-4(3H)-one [00393] 8-bromo-2-[2-(trifluoromethyl)phenyl][l]benzofuro[3,2-d]pyrimidin- 4(3H)-one was synthesized in a manner similar to Example 8, wherein 2- chlorobenzaldehyde was substituted with 2-trifluoromethylbenzaldehyde. IH NMR (400 MHz, d6-DMSO): 13.45 (s, IH), 8.16 (s, IH), 7.81 (m, 6H). MS (EI) for C17 H8 Br F3 N2 O2: 410 (MH+).
(Compound 66) 8-bromo-2-r2-bromo-4,5-bis(methyloxy)phenyliπibenzofuror3,2-dlpyrimidin-4(3H)- one
[00394] 8-bromo-2-[2-bromo-4,5-bis(methyloxy)phenyl][l]benzofuro[3,2- d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 8, wherein 2- chlorobenzaldehyde was substituted with 2-bromo-4,5-dimethoxybenzaldehyde. IH NMR (400 MHz, d6-DMSO): 13.29 (s, IH), 8.26 (s, IH), 7.87 (m, 2H), 7.30 (d, 2H), 3.87 (s, 3H), 3.80 (s, 3H). MS (EI) for C18 H12 Br2 N2 04: 481 (MH+).
(Compound 67)
8-bromo-2-[2-fluoro-5-(methyloxy)phenyll[llbenzofuro[3,2-dlpyrimidin-4(3H)-one [00395] 8-bromo-2-[2-fluoro-5-(methyloxy)phenyl][l]benzofuro[3,2-d]pyrimidin- 4(3H)-one was synthesized in a manner similar to Example 8, wherein 2- chlorobenzaldehyde was substituted with 2-fluoro-5-methoxybenzaldehyde. IH NMR (400 MHz, d6-DMSO): 13.30 (s, br, IH), 8.24 (s, IH), 7.85 (m, 2H), 7.32 (m, 2H), 7.16 (m, IH), 3.80 (s, 3H). MS (EI) for C17 HlO Br F N2 03: 390 (MH+).
(Compound 68) 8-bromo-2-r2-chloro-3 ,4-bis(methyloxy)phenyll [ 1 lbenzofuro[3 ,2-dlpyrimidin-4(3H)- one [00396] 8-bromo-2-[2-chloro-3,4-bis(methyloxy)phenyl][l]benzofuro[3,2- d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 8, wherein 2- chlorobenzaldehyde was substituted with 2-chloro-3,4-dimethoxybenzaldehyde. IH NMR (400 MHz, d6-DMSO): 13.28 (s, IH), 8.24 (s, IH), 7.86 (m, 2H), 7.42 (d, IH),
7.21 (d, IH), 3.92 (s, 3H), 3.81 (s, 3H). MS (EI) for C18 H12 Br Cl N2 04: 437
(MH+).
(Compound 69)
8-bromo-2-(5-chloro-2-thienyl)πibenzofuror3,2-dlpyrimidin-4(3H)-one [00397] 8-bromo-2-(5-chloro-2-thienyl)[l]benzofuro[3,2-d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 8, wherein 2-chlorobenzaldehyde was substituted with 5-chloro-2-thiophenecarboxaldehyde. IH NMR (400 MHz, d6- DMSO): 13.37 (s, IH), 8.18 (s, IH), 8.06 (d, IH), 7.84 (m, 2H), 7.29 (d, IH). MS (EI) for C14 H6 Br Cl N2 02 S: 383 (MH+).
(Compound 70)
8-bromo-2-(2,6-difluorophenyl)πibenzofuror3,2-dlpyrimidin-4(3H)-one [00398] 8-bromo-2-(2,6-difluorophenyl)[l]benzofuro[3,2-d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 8, wherein 2-chlorobenzaldehyde was substituted with 2,6-dichlorobenzaldehyde. IH NMR (400 MHz, d6-DMSO): 13.67 (s, IH), 8.27 (s, IH), 7.89 (m, 2H), 7.71 (m, IH), 7.30 (m, 2H). MS (EI) for C16 H7 Br F2 N2 02: 378 (MH+). (Compound 107)
8-bromo-2-(3 ,5 -dichloropyridin-4-yl)r 1 lbenzofuro[3 ,2-dlpyrimidin-4(3H)-one [00399] 8-bromo-2-(3,5-dichloropyridin-4-yl)[l]benzofuro[3,2-d]pyrimidin-4(3H)- one was synthesized in a manner similar to Example 8, wherein 2- chlorobenzaldehyde was substituted with 3,5-dichloroisonicotinaldehyde. IH NMR (400 MHz, d6-DMSO): 13.81 (s, IH), 8.91 (s, 2H), 8.30 (s, IH), 7.90 (m, 2H). MS (EI) for C15 H6 Br C12 N3 02: 412 (MH+).
(Compound 57)
8-Bromo-2-pyridin-2-yl[llbenzofuro[3,2-dlpyrimidin-4(3H)-one. [00400] 8-Bromo-2-pyridin-2-yl[l]benzofuro[3,2-d]pyrimidin-4(3H)-one was synthesized manner similar to Example 8, wherein 2-chlorobenzaldehyde was substituted with pyridine-2-carboxaldehyde. 1H NMR (400 MHz, d6-DMSO): 12.41 (broad s, IH), 8.77 (d, IH), 8.47 (d, IH), 8.30 (s, IH), 8.16 (dd, IH), 7.88 (d, IH), 7.86 (d, IH), 7.65 (dd, IH). MS (EI) for Ci5 H8 Br N3 O2: 343 (MH+).
(Compound 58) 8-Bromo-2-(2-fiuorophenvnrilbenzofuror3.2-dlpyrimidin-4(3H)-one
[00401] 8-Bromo-2-(2-fiuorophenyl)[l]benzofuro[3,2-d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 8, wherein 2-chlorobenzaldehyde was substituted with 2-fluorobenzaldehyde. 1H NMR (400 MHz, d6-DMSO): 12.42 (broad s, IH), 7.96 (s, IH), 7.89 (d, IH), 7.62 (d, IH), 7.61 (d, IH), 7.53 (dd, IH), 7.46 (s, IH), 7.35 (dd, IH). MS (EI) for Ci6 H8 Br F N2 O2: 360 (MH+).
(Compound 59)
8-bromo-2-(2-thienyl)πibenzofuror3,2-dlpyrimidin-4(3H)-one [00402] 8-Bromo-2-(2-thienyl)[l]benzofuro[3,2-d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 8, wherein 2-chlorobenzaldehyde was substituted with thiophene-2-carboxaldehyde. 1H NMR (400 MHz, d6-DMSO): 12.39 (broad s, IH), 8.23 (d, IH), 7.85 (s, IH), 7.84 (d, IH), 7.69 (d, IH), 7.46 (d, IH), 7.17 (dd, IH). MS (EI) for Ci4 H7 Br N2 O2 S: 348 (MH+).
(Compound 53) methyl 4-(4-oxo-3,4-dihydro[llbenzofuro[3,2-dlpyrimidin-2-yl)benzoate [00403] Methyl 4-(4-oxo-3,4-dihydro[l]benzofuro [3, 2-d]pyrimidin-2-yl)benzoate was synthesized in the same manner as Example 8 wherein 2-chlorobenzaldehyde was replaced with methyl 4-formylbenzoate, and 3-amino-5-bromobenzofuran-2- carboxamide 3 was replaced with 3-amino-benzofuran-2-carboxamide. IH NMR (400 MHz, d6-DMSO): 13.32 (s, IH), 8.30 (d, 2H), 8.11 (m, 3H), 7.86 (d, IH), 7.71 (m, IH), 7.53 (m, IH), 3.90 (s, 3H). MS (EI) for Ci8Hi2N2O4: 321 (MH+).
(Compound 63) 2-(2-chlorophenyl)-8-methviπibenzofuror3,2-dlpyrimidin-4(3H)-one
[00404] 2-(2-chlorophenyl)-8-methyl[l]benzofuro[3,2-d]pyrimidin-4(3H)-one was synthesized in the same manner as Example 8 wherein 3-amino-5-bromobenzofuran- 2-carboxamide 3 was replaced with 3-amino-5-methyl-benzofuran-2-carboxamide. 3- Amino-5-methyl-benzofuran-2-carboxamide was synthesized in the same manner as Example 1 wherein 5-bromo-2-hydroxybenzonitrile 1 was replaced with 5-methyl-2- hydroxybenzonitrile. 5-methyl-2-hydroxybenzonitrile was synthesized in the same manner as Example AAGl wherein 5-bromo-2-hydroxy-3-methylbenzaldehyde was replaced with 2-hydroxy-5-methylbenzaldehyde. IH NMR (400 MHz, d6-DMSO): 13.28 (s, IH), 7.85 (s, IH), 7.76 (d, IH), 7.70 (dd, IH), 7.65 (m, IH), 7.60 (m, IH), 7.53 (m, 2H), 2.48 (s, 3H). MS (EI) for C17H11ClN2O2: 312 (MH+).
(Compound 123)
8-bromo-2-(3-methyl-lH-indazol-5-yl)[llbenzofuro[3,2-dlpyrimidin-4(3H)-one [00405] 8-bromo-2-(3-methyl-lH-indazol-5-yl)[l]benzofuro[3,2-d]pyrimidin- 4(3H)-one was synthesized in a manner similar to Example 8, wherein 2- chlorobenzaldehyde was substituted with 3 -methyl- lH-indazole-5-carbaldehyde (see Note 1 for synthesis). 1H-NMR (400MHz, d6-DMSO): 12.93 (s, IH), 8.69 (s br, IH), 8.26 (m, 2H), 7.83 (s, 2H), 7.58 (d, IH), 2.57 (s, 3H). MS (EI) for Ci8HnBrN4O2:
396 (MH+). [00406] Note 1: 3 -methyl- lH-indazole-5-carbaldehyde and lH-indazole-5- carbaldehyde were synthesized according to a patent published by Piatnitski, Evgueni; Kiselyov, Alexander. Heteroaryl aminophenyl ketone derivatives and their preparation and use as kinase inhibitors, e.g., in the treatment of neoplastic diseases. PCT Int. Appl. (2005), 58 pp. CODEN: PIXXD2 WO 2005000813 Al 20050106 CAN 142:114055 AN 2005:14374 CAPLUS
(Compound 170)
2-(3-methyl-lH-indazol-5-yl)-9-(methyloxy)[llbenzofuro[3,2-dlpyrimidin-4(3H)-one [00407] 2-(3-methyl-lH-indazol-5-yl)-9-(methyloxy)[l]benzofuro[3,2- d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 8, wherein 3- methyl-lH-indazole-5-carbaldehyde (see Note 1 for synthesis) was substituted with 2- chlorobenzaldehyde and Example 1 wherein 6-methoxy-2-hydroxybenzonitrile was substituted with 5-bromo-2-hydroxybenzonitrile 1. 1H-NMR (400MHz, d6-DMSO): 12.66 (s, IH), 8.65 (s br, IH), 8.43 (d, IH), 7.45 (m, 2H), 7.25 (d, IH), 6.89 (d, IH), 4.04 (s, 3H), 2.56 (s, 3H). MS (EI) for Ci9Hi4N4O3: 347 (MH+).
(Compound 171)
2-(3-amino-lH-indazol-5-yl)-9-(methyloxy)[llbenzofuro[3,2-dlpyrimidin-4(3H)-one [00408] 2-(3-amino-lH-indazol-5-yl)-9-(methyloxy)[l]benzofuro[3,2-d]pyrimidin- 4(3H)-one was synthesized in a manner similar to Example 8, wherein 2- chlorobenzaldehyde was substituted with 2-fluoro-5-formylbenzonitrile, and
Example 1, wherein 5-bromo-2-hydroxybenzonitrile (1) was substituted with 6- methoxy-2-hydroxybenzonitrile. Subsequently, resulting crude 2-fluoro-5-(9- methoxy-4-oxo-3,4-dihydrobenzofuro[3,2-d]pyrimidin-2-yl)benzonitrile was dissolved in DMSO (2mL) and hydrazine hydrate (1 mL). The reaction mixture was heated to 110 0C for 16h in a sealed vessel. Upon cooling, the mixture was concentrated in vacuo and dissolved in methanol. Formation of product was confirmed by LC/MS and the product was purified by preparatory HPLC (reverse- phase, acetonitrile/water with 0.1% formic acid) to yeild the title compound. 1H- NMR (400MHz, d6-DMSO): 8.64 (s br, IH), 8.10 (d, IH), 7.62 (t, IH), 7.52 (d, IH), 7.40 (d, IH), 7.05 (d, IH), 4.04 (s, 3H). MS (EI) for Ci8Hi3N5O3: 348 (MH+).
(Compound 220)
2-(3-amino-lH-indazol-5-yl)-8-bromorilbenzofuror3,2-dlpyrimidin-4(3H)-one [00409] 2-(3-amino-lH-indazol-5-yl)-8-bromo[l]benzofuro[3,2-d]pyrimidin- 4(3H)-one was synthesized in a manner similar to Example 8 wherein 2- chlorobenzaldehyde was substituted with 2-fluoro-5-formylbenzonitrile. Subsequently, resulting crude 2-fluoro-5-(8-bromo-4-oxo-3,4-dihydrobenzofuro[3,2- d]pyrimidin-2-yl)benzonitrile was dissolved in DMSO (2mL) and hydrazine hydrate (1 mL). The reaction mixture was heated to 110 0C for 16h in a sealed vessel. Upon cooling the mixture was concentrated in vacuo and dissolved in methanol. Formation of product was confirmed by LC/MS and the product was purified by preparatory HPLC (reverse-phase, acetonitrile/water with 0.1% formic acid) to yeild the title compound. 1H-NMR (400MHz, d6-DMSO): 11.7 (br s, IH), 8.63 (s br, IH), 8.28 (s, IH), 8.20 (m, IH), 8.10 (d, IH), 7.79 (m, 2H), 7.30 (d, IH). MS (EI) for Ci7Hi0BrN5O2: 396 (MH+). EXAMPLE 9
Figure imgf000201_0001
wherein R5 is described within the compounds within this example. 3-Amino-5-chlorobenzofuran-2-carboxylic acid 33
[00410] Ethyl 3-amino-5-chlorobenzofuran-2-carboxylate 32 (1.00 g, 4.17 mmol) was suspended in a solution of 15 mL ethanol and 15 mL of 1.0 M sodium hydroxide. The suspension was heated at 65 0C overnight. After cooling, the clear solution was acidified to pH 3 with concentrated hydrochloric acid. The precipitate was filtered, washed with 2 mL water and dried under vacuum to give 753 mg of white solid. MS (EI) for C9H6ClNO3: 212 (MH+). 3-Amino-5-chlorobenzofuran-2-carboxamide 34 [00411] 3-Amino-5-chlorobenzofuran-2-carboxylic acid 33 (250 mg, 1.18 mmol) and diisopropylethylamine (515 μL, 2.95 mmol) were dissolved in 10 mL of dimethylacetamide. HATU (560 mg, 1.25 mmol) was added and the reaction was stirred for 30 min at room temperature. Ammonia gas was bubbled through the reaction mixture for 1 min. After stirring for another 30 min, the reaction mixture was diluted with 20 mL of ethyl acetate. The reaction mixture was washed three times with 10 mL portions of water and once with 10 mL of saturated aqueous sodium chloride. The organic fraction was separated, dried with magnesium sulfate and concentrated under vacuum to give 220 mg of 3-amino-5-chlorobenzofuran-2- carboxamide. MS (EI) for C9H7ClN2O2: 211 (MH+).
(Compound 31) 8-Chloro-2-(2-chlorophenyl)rilbenzofuror3,2-dlpyrimidin-4(3H)-one
[00412] A suspension of 3-amino-5-chlorobenzofuran-2-carboxamide 34 (150 mg, 0.71 mmol), 2-chlorobenzaldehyde (90 μL, 0.80 mmol) and copper(II) chloride (95 mg, 0.71) in 2.5 mL anhydrous ethanol was heated for 20 min at 120 0C in a microwave reactor. The solvent was concentrated under vacuum. The residue was taken up in 3 mL of dimethylformamide, filtered and purified by preparative HPLC to give 20 mg of 8-chloro-2-(2-chlorophenyl)[l]benzofuro[3,2-d]pyrimidin-4(3H)-one. 1H NMR (400 MHz, d6-DMSO): 13.34 (m, IH), 8.06 (d, IH), 7.88(d, IH), 7.67 (dd, IH), 7.63 (dd, IH), 7.58 (dd, IH), 7.53 (m, IH), 7.46 (m, IH); MS (EI) for Ci6H8Cl2N2O2: 331 (MH+). (Compound 85)
8-bromo-2-(lH-imidazol-2-yl)[llbenzofuro[3,2-dlpyrimidin-4(3H)-one
[00413] 8-bromo-2-(lH-imidazol-2-yl)[l]benzofuro[3,2-d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 9, Compound 31, wherein 2- chlorobenzaldehyde was substituted with lH-imidazole-2-carbaldehyde, and 3-amino- 5-bromobenzofuran-2-carboxamide was substituted for 3-amino-5-chlorobenzofuran- 2-carboxamide. 1H NMR (400 MHz, d6-DMSO): 8.18 (s, IH), 7.81 (m, 2H), 7.28 (m, 2H); MS (EI) for Ci3H7BrN4O2: 332 (MH+).
(Compound 86)
8-bromo-2-(l,3-thiazol-2-yl)πibenzofuror3,2-dlpyrimidin-4(3H)-one [00414] 8-bromo-2-(l,3-thiazol-2-yl)[l]benzofuro[3,2-d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 9, wherein thiazole-2-carbaldehyde replaced 2-chlorobenzaldehyde and 3-amino-5-bromobenzofuran-2-carboxamide replaced 3-amino-5-chlorobenzofuran-2-carboxamide. 1H NMR (400 MHz, d6- DMSO): 8.17 (s, IH), 7.89 (s, IH), 7.67 (m, 3H); MS (EI) for Ci3H6BrN3O2S: 349 (MH+).
(Compound 77)
8-bromo-2-(2-ethylphenyl)[llbenzofuro[3,2-dlpyrimidin-4(3H)-one [00415] 8-bromo-2-(2-ethylphenyl)[l]benzofuro[3,2-d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 9, wherein 3 -amino-5 -bromobenzofuran- 2-carboxamide replaced 3 -amino-5 -chlorobenzofuran-2-carboxamide, and 2- ethylbenzaldehyde replaced 2-chlorobenzaldehyde. IH NMR (400 MHz, d6-DMS0): 13.20 (s, br, IH), 8.20 (s, IH), 7.85 (m, 2H), 7.48 (m, 2H), 7.40 (d, IH), 7.34 (m, IH), 2.74 (m, 2H), 1.12 (t, 3H). MS (EI) for C18 H13 Br N2 02: 370 (MH+).
(Compound 78)
8-bromo-2-[2-bromo-5-(methyloxy)phenyll[llbenzofuro[3,2-dlpyrimidin-4(3H)-one [00416] 8-bromo-2-[2-bromo-5-(methyloxy)phenyl][l]benzofuro[3,2-d]pyrimidin- 4(3H)-one was synthesized in a manner similar to Example 9, wherein 3-amino-5- bromobenzofuran-2-carboxamide replaced 3 -amino-5 -chlorobenzofuran-2- carboxamide, and 2-bromo-5-methoxybenzaldehyde replaced 2-chlorobenzaldehyde. IH NMR (400 MHz, d6-DMSO): 13.37 (s, br, IH), 8.23 (s, IH), 7.85 (m, 2H), 7.57 (m, IH), 7.26 (s, IH), 7.10 (m, IH), 3.80 (s, 3H). MS (EI) for C17 HlO Br2 N2 03: 451 (MH+).
(Compound 79)
8-bromo-2-[2-chloro-4-(methyloxy)phenyll[llbenzofuro[3,2-dlpyrimidin-4(3H)-one [00417] 8-bromo-2-[2-chloro-4-(methyloxy)phenyl][l]benzofuro[3,2-d]pyrimidin- 4(3H)-one was synthesized in a manner similar to Example 9, wherein 3-amino-5- bromobenzofuran-2-carboxamide replaced 3 -amino-5 -chlorobenzofuran-2- carboxamide, and 2-chloro-4-methoxybenzaldehyde replaced 2-chlorobenzaldehyde. IH NMR (400 MHz, d6-DMSO): 13.30 (s, IH), 8.25 (s, IH), 7.87 (d, 2H), 7.63 (d, IH), 7.22 (s, IH), 7.09 (dd, IH), 3.86 (s, 3H). MS (EI) for C17 HlO Br Cl N2 03: 407 (MH+).
EXAMPLE 10
Figure imgf000204_0001
36 37
Figure imgf000204_0002
wherein R5 is described within the compounds within this example. 2-Hydroxy-5-methoxybenzonitrile 37 [00418] To a suspension of sodium acetate (10.8 g, 131.7 mmol) in 10 mL of acetic acid was added 2-hydroxy-5-methoxybenzaldehyde 36 (10.0 g, 65.72 mmol) and nitroethane (4.7 mL, 65.8 mmol). The mixture was refluxed overnight, cooled to room temperature, then diluted with ethyl acetate and water (200 mL each). The organic layer was separated. The aqueous layer was extracted with ethyl acetate (2X50 mL). The combined organic was washed with saturated sodium bicarbonate solution
(3X100 mL), brine (100 mL), dried over anhydrous sodium sulfate, and concentrated to a smaller volume. 1.2 g of crystalline powder was collected by filtration after standing overnight, washed with 20 mL of tert-butyl methyl ether. The filtrate was purified with a silica gel column (5% to 25% ethyl acetate in hexanes), 6.3 g of yellowish powder was obtained as the desired product. 1H NMR (400 MHz, d6-
DMSO): 10.20 (s, IH), 6.93 - 6.96 (m, 3H), 3.75 (s, 3H); MS (EI) for C8H7NO2: 150 (MH+).
3-Amino-5-methoxybenzofuran-2-carboxamide 38 [00419] To a suspension of 2-hydroxy-5-methoxybenzonitrile 37 (6.3 g, 42.3 mmol), cesium carbonate (20.6 g, 63.45 mmol) in 70 mL of acetonitrile was added 2- chloroacetamide (4.2 g, 44.42 mmol) and potassium iodide (2 g, 12.0 mmol). The suspension was heated to 80 0C overnight, then cooled to room temperature. The suspension was filtered through a Celite pat and washed with 150 mL of ethyl acetate. The filtrate was concentrated, the solid residue was suspended in 20 mL of methanol, and filtered again, washed with 10 mL of methanol. 3.9 g of yellowish crystalline powder was collected as the desired product. 1H NMR (400 MHz, d6-DMSO): 7.39 (s, IH), 7.30 (d, IH), 7.10 (br, 2H), 6.98 (d, 2H), 5.90 (s, 2H), 3.78 (s, 3H); MS (EI) for
CiOHi0N2O3: 207 (MH+).
(Compound 32) 2-(2-Chlorophenyl)-8-(methyloxy)rilbenzofuror3,2-dlpyrimidin-4(3H)-one
[00420] To a suspension of 3-amino-5-methoxybenzofuran-2-carboxamide 38 (1.0 g, 4.85 mmol) and 2-chlorobenzaldehyde (1.7 g, 9.70 mmol) in 20 mL anhydrous ethanol was added 2 drops of concentrated HCl. The mixture was stirred overnight at room temperature, and then 20 mL DMSO was added. The reaction mixture was heated to 150 0C while the flask was opened to the air. Sodium bisulfite (2.5 g, 24.25 mmol) was added in several portions. The mixture was cooled to room temperature after 5 h, diluted with 100 mL of water, stirred for 0.5 h. The aqueous suspension was filtered, washed with 20 mL of water, dried in the air. 1.3 g of yellow-brown powder was collected as the desired product. 1H NMR (400 MHz, d6-DMSO): 13.28 (s, IH), 7.80 (d, IH), 7.72 (d, IH), 7.58 - 7.64 (m, 2H), 7.52 (m, 2H), 7.28 (d, IH), 3.82 (s, 3H); MS (EI) for CI7HI IC1N2O3: 327 (MH+).
(Compound 71)
2-(2-chlorophenyl)-7-hydroxy[llbenzofuro[3,2-dlpyrimidin-4(3H)-one [00421] 2-(2-chlorophenyl)-7-hydroxy[l]benzofuro[3,2-d]pyrimidin-4(3H)-one was synthesized in the same manner as Example 10 wherein 2-hydroxy-5- methoxybenzaldehyde was replaced with 2-hydroxy-4-methoxybenzaldehyde. The resulting 2-(2-chlorophenyl)-7-methoxy [ 1 ]benzofuro [3 ,2-d]pyrimidin-4(3H)-one (160 mg, 0.49 mmol) was dissolved in 20 mL of Dichloroethane and BBr3Me2S (0.3 g, 1 mmol) was added. The reaction mixture was heated to reflux for 8h, cooled down to room temperature and 5 mL of water was added. The organic phase was washed with brine, dried over Na2SO4 and concentrated in vacuo to afford 142 mg (93%) of the title compound. IH NMR (400 MHz, d6-DMSO): 13.22 (s, br, IH), 10.41 (s, br, IH), 7.83 (d, IH), 7.68 (m, IH), 7.63 (m, IH), 7.59 (m, IH), 7.51 (m, IH), 7.13 (d, IH), 6.98 (dd, IH). MS (EI) for C16H9C1N2O3: 314 (MH+). (Compound 73)
2-(2-chlorophenyl)-8-hvdroxyrilbenzofuror3,2-dlpyrimidin-4(3H)-one [00422] 2-(2-chlorophenyl)-8-hydroxy[l]benzofuro[3,2-d]pyrimidin-4(3H)-one was synthesized in the same manner as in Example 10. The resulting 2-(2- Chlorophenyl)-8-(methyloxy)[l]benzofuro[3,2-d]pyrimidin-4(3H)-one (Compound 32) (160 mg, 0.49 mmol) was dissolved in 20 mL of Dichloroethane and BBrSMe2S (0.3 g, 1 mmol) was added. The reaction mixture was heated to reflux for 8h, cooled down to room temperature and 5 mL of water was added. The organic phase was washed with brine, dried over Na2SO4 and concentrated in vacuo to afford 135 mg (90%) of the title compound.
IH NMR (400 MHz, d6-DMSO): 13.22 (s, IH), 9.75 (m, IH), 7.59 (m, 4H), 7.24 (m, IH), 7.08 (m, IH), 5.76 (m, IH). MS (EI) for C16H9C1N2O3: 314 (MH+). (Compound 83)
2-(2-chlorophenyl)-9-(methyloxy)[llbenzofuro[3,2-dlpyrimidin-4(3H)-one [00423] 2-(2-chlorophenyl)-9-(methyloxy)[l]benzofuro[3,2-d]pyrimidin-4(3H)- one was synthesized in the same manner as Example 10 wherein 2-hydroxy-5- methoxybenzaldehyde was replaced with 2-hydroxy-6-methoxylbenzaldehyde. IH NMR (400 MHz, d6-DMSO): 13.28 (s, IH), 7.68 (dd, IH), 7.62 (m, 3H), 7.52 (m, 2H), 7.41 (d, IH), 7.01 (d, IH), 3.95 (s, 3H). MS (EI) for C17H11C1N2O3: 328 (MH+).
(Compound 124)
2-(2-chloro-4-nitrophenyl)-8-(methyloxy)πibenzofuror3,2-dlpyrimidin-4(3H)-one [00424] 2-(2-Chloro-4-nitrophenyl)-8-(methyloxy)[l]benzofuro[3,2-d]pyrimidin- 4(3H)-one was synthesized in the same manner as Example 10 wherein 2- chlorobenzaldehyde was replaced with 2-chloro-4-nitrobenzaldehyde. IH NMR (400 MHz, d6-DMSO): 13.52 (s, IH), 8.50 (s, IH), 8.37 (d, IH), 8.03 (d, IH), 7.81 (d, IH), 7.53 (s, IH), 7.29 (d, IH), 3.86 (s, 3H). MS (EI) for C17H10C1N3O5: 373 (MH+). (Compound 129)
2-(4-amino-2-chlorophenyl)-8-(methyloxy)[llbenzofuro[3,2-dlpyrimidin-4(3H)-one [00425] 2-(4-amino-2-chlorophenyl)-8-(methyloxy)[l]benzofuro[3,2-d]pyrimidin- 4(3H)-one was synthesized in the same manner as in Example 10 and wherein 2- chlorobenzaldehyde was replaced with 2-chloro-4-nitrobenzaldehyde. IH NMR (400 MHz, d6-DMSO): 13.26 (s, br, IH), 7.77 (d, IH), 7.48 (m, IH), 7.37 (m, IH), 7.26 (m, IH), 6.77 (s, IH), 6.66 (m, IH), 3.86 (s, 3H). MS (EI) for C17H12C1N3O3: 343 (MH+). (Compound 137) lJ-dimethylethyl 4-[({3-chloro-4-[8-(methyloxy)-4-oxo-3,4- dihydro [ 1 lbenzofuro [3 ,2-d1pyrimidin-2-vHphenvU amino)methyllpiperidine- 1 - carboxylate [00426] 1,1-dimethylethyl 4-[({3-chloro-4-[8-(methyloxy)-4-oxo-3,4- dihydro [ 1 ]benzofuro [3 ,2-d]pyrimidin-2-yl]phenyl} amino)methyl]piperidine- 1 - carboxylate was synthesized in the same manner as in Example 10 wherein 2- chlorobenzaldehyde was replaced with 2-chloro-4-nitrobenzaldehyde. IH NMR (400 MHz, d6-DMSO): 7.35 (d, IH), 7.57 (m, 2H), 7.22 (m, 3H), 6.64 (m, 2H), 4.12 (m, IH), 3.91 (s, 3H), 3.10 (d, 2H), 2.71 (m, 2H), 1.78 (m, 4H), 1.46 (s, 9H), 1.21 (m, 2H). MS (EI) for C28H31C1N4O5: 540 (MH+).
(Compound 138)
2-{2-chloro-4-r(piperidin-4-ylmethyl)aminolphenvU-8-(methyloxy)rilbenzofuror3,2- dlpyrimidin-4(3H)-one [00427] 2- {2-chloro-4-[(piperidin-4-ylmethyl)amino]phenyl} -8-
(methyloxy)[l]benzofuro[3,2-d]pyrimidin-4(3H)-one was synthesized in the same manner as in Example 10 wherein 2-chlorobenzaldehyde was replaced with 2-chloro- 4-nitrobenzaldehyde. IH NMR (400 MHz, d6-DMSO): 8.93 (br s, IH), 8.66 (br s, IH), 7.74 (d, IH), 7.43 (d, IH), 7.36 (d, IH), 7.23 (dd, IH), 6.70 (d, IH), 6.63 (dd, IH), 4.10 (m, 2H), 3.71 (s, 3H), 3.23 (m, 2H), 2.99 (m, 2H), 2.83 (m, 2H), 1.82 (m, 3H), 1.37 (m, 2H). MS (EI) for C23H23C1N4O3: 440 (MH+).
(Compound 144)
2-(2-chloro-4-fluorophenyl)-8-(methyloxy)πibenzofuror3,2-dlpyrimidin-4(3H)-one [00428] 2-(2-chloro-4-fluorophenyl)-8-(methyloxy)[l]benzofuro[3,2-d]pyrimidin- 4(3H)-one was synthesized in the same manner as Example 10 wherein 2- chlorobenzaldehyde was replaced with 2-chloro-4-fluorobenzaldehyde. IH NMR (400 MHz, d6-DMSO): 13.32 (s, IH), 7.79 (m, 2H), 7.68 (dd, IH), 7.52 (d, IH), 7.42 (m, IH), 7.28 (dd, IH), 3.86 (s, 3H). MS (EI) for C17H10C1FN2O3: 346 (MH+). (Compound 145)
2-(2-chloro-5-nitrophenyl)-8-(methyloxy)[llbenzofuro[3,2-dlpyrimidin-4(3H)-one [00429] 2-(2-chloro-5-nitrophenyl)-8-(methyloxy)[l]benzofuro[3,2-d]pyrimidin-
4(3H)-one was synthesized in the same manner as Example 10 wherein 2- chlorobenzaldehyde was replaced with 2-chloro-5-nitrobenzaldehyde. IH NMR (400 MHz, d6-DMSO): 13.45 (s, IH), 8.63 (d, IH), 8.43 (dd, IH), 7.97 (d, IH), 7.81 (d, IH), 7.53 (d, IH), 7.29 (dd, IH), 3.86 (s, 3H). MS (EI) for C17H10C1N3O5: 373 (MH+).
(Compound 152)
2-(3-chloropyridin-4-yl)-8-(methyloxy)[llbenzofuro[3,2-dlpyrimidin-4(3H)-one [00430] 2-(3-chloropyridin-4-yl)-8-(methyloxy)[l]benzofuro[3,2-d]pyrimidin- 4(3H)-one was synthesized in the same manner as Example 10 wherein 2- chlorobenzaldehyde was replaced with 3-chloroisonicotinaldehyde. IH NMR (400 MHz, d6-DMSO): 10.84 (s, IH), 8.75 (d, 2H), 8.09 (s, IH), 7.78 (m, 2H), 7.52 (m, IH), 7.37 (s, IH), 7.12 (m, IH), 3.78 (s, 3H). MS (EI) for C16H10C1N3O3: 329 (MH+).
EXAMPLE 11
Figure imgf000208_0001
wherein R3a is as defined in the disclosure, R15 is described within the compounds within this example, and Rn is hydrogen, heterocycloalkylalkyl or dialkylaminoalkyl. 2-Chloro-4-nitrobenzaldehyde [00431] To a suspension OfNaBH4 (2.1g, 56.6mmol) in 1 ,2-dimethoxy ethane (30 niL) was added 2-chloro-4-nitrobenzoyl chloride (5g, 22.8 mmol) at OC, and the reaction mixture was stirred at RT for 2h. The mixture was concentrated in vacuo. Water was added to the residue and the mixture was extracted with EtOAc. The organic layer was washed with IN HCl, IN NaOH, water, brine, and dried over
MgSO4, and concentrated on rotary evaporator. The residue was dissolved in 50 mL of Acetone and activated MnO2 (10 g) was added. The reaction mixture was stirred for 18h at RT. The slurry was filtered and concentrated down. Purification by column chromatography resulted in 1.2 g (29%) of 2-chloro-4-nitrobenzaldehyde. (Compound 33)
8-Bromo-2-(2-chloro-4-nitrophenyl)[llbenzofuro[3,2-dlpyrimidin-4(3H)-one. [00432] A mixture of 2-chloro-4-nitrobenzaldehyde (1.2 g, 6.4mmol), compound 3 (0.7g, 2.7mmol), NaHSO3 ( 2g, 19 mmol) in 10 mL of DMSO was heated to 150 0C for 5h. Upon completion, the reaction mixture was cooled down to RT, and 10 mL of water was added. The precipitate was filtered off, resulting in Ig (88%) of 8-bromo-2- (2-chloro-4-nitrophenyl)[l]benzofuro[3,2-d]pyrimidin-4(3H)-one. The product was submitted to the next step without further purification. 1H NMR (400 MHz, d6- DMSO): 13.62 (s, IH), 8.52 (d, IH), 8.37 (dd, IH), 8.27 (d, IH), 8.02 (d, IH), 7.89 (m, 2H); MS (EI) for Ci6H7BrClN3O4: 422 (MH+). (Compound 34)
2-(4-Amino-2-chlorophenyl)-8-bromo[llbenzofuro[3,2-dlpyrimidin-4(3H)-one. [00433] A mixture of 8-bromo-2-(2-chloro-4-nitrophenyl)[l]benzofuro[3,2- d]pyrimidin-4(3H)-one (0.4 g, 0.95 mmol), Tin(II) chloride dihydrate (3g, 13.3 mmol), Methanol (5mL), EtOAc (2OmL), and water (ImL) was heated to 80 0C for Ih. The resulting slurry was concentrated down on the rotary evaporator, and extracted with EtOAc/ water mixture. The organic layer was dried over magnesium sulfate, and concentrated down, resulting in 2-(4-amino-2-chlorophenyl)-8- bromo[l]benzofuro[3,2-d]pyrimidin-4(3H)-one that was submitted to the next step without further purification. The aliquot of the product was purified by preparative HPLC. 1H NMR (400 MHz, d6-DMSO): 8.19 (m, IH), 7.83 (m, 2H), 7.33 (d, IH), 6.69 (m, IH), 6.58 (m, IH), 5.86 (m, 2H); MS (EI) for Ci6H9BrClN3O2: 391 (MH+). (Compound 35)
8-bromo-2-{2-chloro-4-[(piperidin-4-ylmethyl)aminolphenyl|[llbenzofuro[3,2- d1pyrimidin-4(3H)-one
[00434] To the solution of 2-(4-amino-2-chlorophenyl)-8-bromo[l]benzofuro[3,2- d]pyrimidin-4(3H)-one (o.3g, 0.76 mmol) in 1OmL of 1 :1 Dichloromethane/Dimethylformamide was added tert-butyi 4-formylpiperidine-l- carboxylate (0.5 g, 2.3mmol), and sodium triacetoxyborohydride (Ig, 4.7mmol) at room temperature. The reaction mixture was heated to 60 0C for 1 h, then it was concentrated under reduced pressure, re-dissolved in 10 mL of MeOH. To the resulting slurry was added 2 mL of 4 N HCl in Dioxane. The reaction mixture was heated to 50 0C for Ih, concentrated under reduced pressure. The residue was purified by preparative HPLC, resulting in 11 mg of 8-bromo-2-{2-chloro-4- [(piperidin-4-ylmethyl)amino]phenyl}[l]benzofuro[3,2-d]pyrimidin-4(3H)-one. 1H NMR (400 MHz, d6-DMSO): 8.10 (m, IH), 7.74 (m, 2H), 7.34 (d, IH), 6.64 (m, IH), 6.58 (dd, IH), 6.27 (m, IH), 3.07 (m, 2H), 2.94 (m, 2H), 2.55 (m, 2H), 1.75 (m, 2H), 1.15 (m, 2H); MS (EI) for Ci6C22H20BrCIN4O2 488(MH+).
(Compound 139)
8-bromo-2-{2-chloro-4-r(lH-imidazol-4-ylmethyl)aminolphenvUπibenzofuror3,2- dlpyrimidin-4(3H)-one [00435] 8-bromo-2-{2-chloro-4-[(lH-imidazol-4-ylmethyl)amino]phenyl}- [l]benzofuro[3,2-d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 11 wherein lH-imidazole-4-carbaldehyde replaced tert-butyi A- formylpiperidine-1-carboxylate. 1H NMR (400 MHz, d6-DMSO): 8.17 (d, 2H), 7.83 (t, 2H), 7.60 (s, IH), 7.37 (d, IH), 6.99 (s, IH), 6.75 (m, 3H), 4.22 (s, 2H); MS (EI) for C20Hi3BrClN5O2: 472 (MH+).
(Compound 154)
8-bromo-2-{2-chloro-4-r(pyrrolidin-3-ylmethyl)aminolphenvUπibenzofuror3,2- d"|pyrimidin-4(3H)-one [00436] 8-bromo-2-{2-chloro-4-[(pyrrolidin-3- ylmethyl)amino]phenyl}[l]benzofuro[3,2-d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 11 wherein tert-butyl 3-formylpyrrolidine-l-carboxylate replaced tert-butyl 4-formylpiperidine-l-carboxylate. 1H NMR (400 MHz, d6- DMSO): 8.39 (s, IH), 8.16 (s, IH), 7.9 (m, 2H), 7.37 (d, IH), 6.7 (s, IH), 6.63 (d, IH), 6.48 (m, IH), 3.28 (m, 3H), 3.11 (m, 3H), 2.88 (m, 2H), 2.04 (m, IH), 1.62 (m, IH); MS (EI) for C2IHi8BrClN4O2: 475 (MH+).
(Compound 155) 8-bromo-2-{2-chloro-4-r(2-piperidin-3-ylethyl)aminolphenvUrHbenzofuror3,2- dlpyrimidin-4(3H)-one
[00437] 8-bromo-2-{2-chloro-4-[(2-piperidin-3- ylethyl)amino]phenyl}[l]benzofuro[3,2-d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 11, wherein tert-butyl 3-(2-oxoethyl)piperidine-l- carboxylate replaced tert-butyl 4-formylpiperidine-l-carboxylate. 1H NMR (400
MHz, de-DMSO): 8.39 (s, IH), 8.15 (s, IH), 7.80 (m, 2H), 7.37 (d, IH), 6.67 (s, IH), 6.60 (d, IH), 6.32 (s, IH), 3.15 (m, 6H), 2.67 (t, IH), 1.74 (m, 3H), 1.38 (m, 3H); MS (EI) for C23H22BrClN4O2: 503 (MH+).
(Compound 179) 8-bromo-2-(2-chloro-4-(r3-(dimethylaminoV2.2- dimethylpropyll amino I phenyl) [ 1 lbenzofuro [3 ,2-dlpyrimidin-4(3H)-one [00438] 8-bromo-2-(2-chloro-4- {[3-(dimethylamino)-2,2- dimethylpropyl] amino } phenyl) [ 1 Jbenzofuro [3 ,2-d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 11, wherein 3-(dimethylamino)-2,2- dimethylpropanal replaced tert-butyl 4-formylpiperidine-l -carboxylate. 1H NMR (400 MHz, de-DMSO): 8.33 (s, IH), 8.19 (s, IH), 7.83 (m, 2H) 7.37 (d, IH), 6.71 (m, 2H), 6.27 (s, IH), 2.94 (s, 2H), 2.24 (s, 6H), 2.19 (s, 2H), 0.93 (s, 6H); MS (EI) C23H24BrClN4O2: 505 (MH+).
(Compound 136) 8-bromo-2-(2-chloro-4-{r4-(dimethylamino)butyllamino|phenyl)πibenzofuror3,2- d"|pyrimidin-4(3H)-one [00439] 8-bromo-2-(2-chloro-4- {[4-
(dimethylamino)butyl]amino}phenyl)[l]benzofuro[3,2-d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 11, wherein 4-dimethylamino- butyraldehyde replaced tert-butyl 4-formylpiperidine-l -carboxylate. IH NMR (400 MHz, d6-DMSO): 8.23 (s, br, IH), 8.13 (s, IH), 7.77 (m, 2H), 7.30 (d, IH), 6.61 (s, IH), 6.55 (dd, IH), 3.02 (m, 2H), 2.22 (m, 2H), 2.10 (s, 6H), 1.47 (m, 4H). MS (EI) for C22 H22 Br Cl N4 02: 491 (MH+). (Compound 159)
8-bromo-2-{2-chloro-4-[(piperidin-3-ylmethyl)aminolphenyl|[llbenzofuro[3,2- dlpyrimidin-4(3H)-one
[00440] 8-bromo-2-{2-chloro-4-[(piperidin-3- ylmethyl)amino]phenyl} [l]benzofuro[3,2-d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 11, wherein tert-butyi 3-formylpiperidine-l-carboxylate replaced tert-butyi 4-formylpiperidine-l-carboxylate. IH NMR (400 MHz, d6- DMSO): 8.29 (s, IH), 8.10 (s, IH), 7.74 (m, 2H), 7.31 (d, IH), 6.63 (s, IH), 6.56 (d, IH), 6.40 (m, IH), 3.13 (m, 2H), 3.03 (m, 2H), 2.94 (m, 2H), 2.58 (m, IH), 1.77 (m, IH), 1.66 (m, IH), 1.45 (m, IH), 1.40 (m, IH). MS (EI) for C22 H20 Br C1 N4 02: 489 (MH+).
(Compound 197)
8-bromo-2-{2-chloro-4-r(2-piperidin-4-ylethyl)aminolphenvUrilbenzofuror3,2- dlpyrimidin-4(3H)-one [00441] 8-bromo-2-{2-chloro-4-[(2-piperidin-4- ylethyl)amino]phenyl}[l]benzofuro[3,2-d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 11, wherein tert-butyl 4-(2-oxoethyl)piperidine-l- carboxylate replaced tert-butyl 4-formylpiperidine-l-carboxylate. IH NMR (400 MHz, d6-DMSO): 9.10 (s, br, IH), 8.89 (s, br, IH), 8.22 (s, IH), 7.86 (m, 2H), 7.41 (d, IH), 6.76 (s, IH), 6.69 (d, IH), 3.25 (d, 2H), 3.13 (m, 2H), 2.82 (m, 2H), 2.02 (d, 2H), 1.69 (m, IH), 1.53 (m, 2H), 1.39 (m, 2H). MS (EI) for C23 H22 Br Cl N4 02: 503 (MH+). EXAMPLE HB: Example 11+ coupling
Figure imgf000212_0001
(Compound 140)
N-[4-(8-bromo-4-oxo-3,4-dihydro[llbenzofuro[3,2-dlpyrimidin-2-yl)-3- chlorophenyllpiperidine-3-carboxamide
[00442] N-[4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-3- chlorophenyl]piperidine-3-carboxamide was synthesized in a manner similar to Example 11+coupling, wherein l-(tert-butoxycarbonyl)piperidine-3-carboxylic acid replaced 3-dimethylpropionic acid. The resulting product was dissolved 2 mL of 4 N HCl in Dioxane and 4 mL of EtOAc. The mixture was heated to 50 0C for Ih, concentrated under reduced pressure. The residue was purified by preparative HPLC, resulting in the title compound. 1H NMR (400 MHz, d6-DMSO): 13.35 (s, IH), 10.76 (s, IH), 8.23 (s, IH), 7.99 (s, IH), 7.88 (m, 2H), 7.64 (m, 2H), 3.19 (m, IH), 3.06 (m, IH), 2.94 (m, 2H), 2.08 (m, IH), 1.75 (m, 4H); MS (EI) for C22Hi8BrClN4O3: 503 (MH+).
(Compound 115) N-r4-(8-bromo-4-oxo-3,4-dihvdrorilbenzofuror3,2-dlpyrimidin-2-yl)-3- chlorophenyllpiperidine-4-carboxamide
[00443] N-[4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-3- chlorophenyl]piperidine-4-carboxamide was synthesized in a manner similar to Example 11+coupling, wherein l-(tert-butoxycarbonyl)piperidine-4-carboxylic acid replaced 3-dimethylpropionic acid. The resulting product was dissolved 2 mL of 4 N HCl in Dioxane and 4 mL of EtOAc. The mixture was heated to 50 0C for Ih, concentrated under reduced pressure. The residue was purified by preparative HPLC, resulting in the title compound. 1H NMR (400 MHz, d6-DMSO): 10.40 (s, IH), 8.35 (s, IH), 8.15 (s, IH), 7.93 (s, IH), 7.78 (m, 2H), 7.57 (m, 2H), 3.25 (d, 3H), 2.82 (t, 2H), 1.92 (m, 2H), 1.75 (m, 2H); MS (EI) for C22Hi8BrClN4O3: 503 (MH+).
(Compound 132)
N-[4-(8-bromo-4-oxo-3,4-dihydro[llbenzofuro[3,2-dlpyrimidin-2-yl)-3- chlorophenvHpyrrolidine-3-carboxamide [00444] N-[4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-3- chlorophenyl]pyrrolidine-3-carboxamide was synthesized in a manner similar to
Example 11+coupling, wherein l-(tert-butoxycarbonyl)pyrrolidine-3-carboxylic acid replaced 3-dimethylpropionic acid. The resulting product was dissolved 2 mL of 4 N HCl in Dioxane and 4 mL of EtOAc. The mixture was heated to 50 0C for Ih, concentrated under reduced pressure. The residue was purified by preparative HPLC, resulting in the title compound. 1U NMR (400 MHz, d6-DMSO): 10.51 (s, IH), 8.34 (s, IH), 8.16 (s, IH), 7.90 (s,lH), 7.78 (m, 2H), 7.58 (m, 2H), 3.41 (m, 2H), 3.20 (m, 6H), 2.02 (m, IH); MS (EI) for C2IHi6BrClN4O3: 489 (MH+). (Compound 127)
N-[4-(8-bromo-4-oxo-3,4-dihydro[llbenzofuro[3,2-dlpyrimidin-2-yl)-3- chlorophenyll -N ' 2 ' ,N ' 2 ' -dimethylglycinamide
[00445] N-[4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-3- chlorophenyl]-N'2',N'2'-dimethylglycinamide was synthesized in a manner similar to Example 11 + Coupling, wherein dimethylaminoacetyl chloride replaced 3- dimethylaminopropionic acid chloride. IH NMR (400 MHz, d6-DMSO): 10.03 (s, IH), 8.25 (s, IH), 8.07 (s, IH), 7.91 (s, IH), 7.70 (m, 2H), 7.61 (dd, IH), 7.49 (d, IH), 3.06 (s, 2H), 2.23 (s, 6H). MS (EI) for C20 H16 Br Cl N4 03: 477 (MH+). (Compound 143)
N-[4-(8-bromo-4-oxo-3,4-dihydro[llbenzofuro[3,2-dlpyrimidin-2-yl)-3- chlorophenyllpyridine-4-carboxamide
[00446] N-[4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-3- chlorophenyl]pyridine-4-carboxamide was synthesized in a manner similar to Example 11 + Coupling, wherein isonicotinoyl chloride replaced 3- dimethylaminopropionic acid chloride. IH NMR (400 MHz, d6-DMSO): 10.87 (s, IH), 8.84 (d, 2H), 8.24 (s, IH), 8.12 (s, IH), 7.91 (d, 2H), 7.86 (m, 3H), 7.71 (d, IH). MS (EI) for C22 H12 Br Cl N4 03: 497 (MH+).
(Compound 160) N-[4-(8-bromo-4-oxo-3,4-dihydro[llbenzofuro[3,2-dlpyrimidin-2-yl)-3- chlorophenyll-4-(dimethylamino)butanamide [00447] N-[4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-3- chlorophenyl]-4-(dimethylamino)butanamide was synthesized in a manner similar to
Example 11 + Coupling, wherein 4-dimethylaminobutyric acid replaced 3- dimethylaminopropionic acid. IH NMR (400 MHz, d6-DMSO): 10.40 (s, IH), 8.22
(m, 2H), 7.99 (s, IH), 7.86 (m, 2H), 7.61 (m, 2H), 2.44 (m, 4H), 2.30 (s, 6H), 1.79 (m,
2H). MS (EI) for C22 H20 Br Cl N4 03: 505 (MH+).
(Compound 162)
N-r4-(8-bromo-4-oxo-3,4-dihvdrorilbenzofuror3,2-dlpyrimidin-2-yl)-3- chlorophenyll-3-(lH-imidazol-4-yl)propanamide
[00448] N-[4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-3- chlorophenyl]-3-(lH-imidazol-4-yl)propanamide was synthesized in a manner similar to Example 11 + Coupling, wherein 3-(imidazol-4-yl)propionic acid replaced 3- dimethylaminopropionic acid. IH NMR (400 MHz, d6-DMSO): 10.34 (s, IH), 8.21 (s, 2H), 8.12 (s, IH), 7.89 (s, IH), 7.75 (m, 2H), 7.51 (m, 2H), 7.46 (s, IH), 6.72 (s, IH), 2.77 (m, 2H), 2.61 (m, 2H). MS (EI) for C22 H15 Br C1 N5 03: 514 (MH+).
(Compound 181) N-r4-(8-bromo-4-oxo-3,4-dihvdrorilbenzofuror3,2-dlpyrimidin-2-yl)-3- chlorophenyll-3-piperidin-l-ylpropanamide
[00449] N-[4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-3- chlorophenyl]-3-piperidin-l-ylpropanamide was synthesized in a manner similar to Example 11 + Coupling, wherein 1-piperidinepropionic acid replaced 3- dimethylaminopropionic acid. IH NMR (400 MHz, d6-DMSO): 10.56 (s, IH), 8.23 (dd, IH), 8.20 (s, IH), 7.97 (d, IH), 7.86 (m, 2H), 7.62 (d, IH), 7.56 (dd, IH), 2.66 (m, 2H), 2.54 (m, 2H), 2.44 (m, 4H), 1.52 (m, 4H), 1.40 (m, 2H). MS (EI) for C24 H22 Br Cl N4 03: 531 (MH+).
(Compound 182) N-r4-(8-bromo-4-oxo-3,4-dihvdrorilbenzofuror3,2-dlpyrimidin-2-yl)-3- chlorophenyll-2-(lH-imidazol-4-yl)acetamide [00450] N-[4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-3- chlorophenyl]-2-(lH-imidazol-4-yl)acetamide was synthesized in a manner similar to
Example 11 + Coupling, wherein 4-imidazoleacetic acid replaced 3- dimethylaminopropionic acid. IH NMR (400 MHz, d6-DMSO): 10.55 (s, IH), 8.29
(s, IH), 8.19 (s, IH), 7.99 (s, IH), 7.85 (m, 2H), 7.61 (m, 3H), 6.97 (s, IH), 3.64 (s,
2H). MS (EI) for C21 H13 Br Cl N5 03: 500 (MH+).
(Compound 248)
N-r4-(8-bromo-4-oxo-3,4-dihvdrorilbenzofuror3,2-dlpyrimidin-2-yl)-3- chlorophenvH-3-morpholin-4-ylpropanamide
[00451] N-[4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-3- chlorophenyl]-3-morpholin-4-ylpropanamide was synthesized in a manner similar to Example 11 + Coupling, wherein 3-morpholin-4-yl-propionic acid replaced 3- dimethylaminopropionic acid. IH NMR (400 MHz, d6-DMSO): 10.43 (s, IH), 8.27 (s, IH), 8.17 (s, IH), 7.98 (s, IH), 7.86 (m, 2H), 7.60 (m, 2H), 3.58 (t, 4H), 2.65 (t, 2H), 2.53 (t, 2H), 2.41 (t, 4H). MS (EI) for C23 H20 Br Cl N4 04: 533 (MH+). (Compound 249)
N-[4-(8-bromo-4-oxo-3,4-dihydro[llbenzofuro[3,2-dlpyrimidin-2-yl)-3- chlorophenyllbenzamide
[00452] N-[4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-3- chlorophenyljbenzamide was synthesized in a manner similar to Example 11 + Coupling, wherein benzoyl chloride replaced 3-dimethylaminopropionic acid chloride. IH NMR (400 MHz, d6-DMSO): 13.39 (s, IH), 10.67 (s, IH), 8.26 (s, IH), 8.16 (s, IH), 8.01 (d, 2H), 7.88 (m, 3H), 7.63 (m, 4H). MS (EI) for C23 H13 Br C1 N3 03: 496 (MH+). (Compound 250)
N-[4-(8-bromo-4-oxo-3,4-dihydro[llbenzofuro[3,2-dlpyrimidin-2-yl)-3- chlorophenyllcyclohexanecarboxamide
[00453] N-[4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-3- chlorophenyljcyclohexanecarboxamide was synthesized in a manner similar to Example 11 + Coupling, wherein cyclohexanecarbonyl chloride replaced 3- dimethylaminopropionic acid chloride. IH NMR (400 MHz, d6-DMSO): 13.34 (s, br, IH), 10.25 (s, IH), 8.23 (s, IH), 7.95 (s, IH), 7.85 (m, 2H), 7.60 (s, 2H), 2.37 (m, IH), 1.80 (m, 4H), 1.32 (m, 6H). MS (EI) for C23 H19 Br C1 N3 03: 502 (MH+).
(Compound 251) N-[4-(8-bromo-4-oxo-3,4-dihydro[llbenzofuro[3,2-dlpyrimidin-2-yl)-3- chlorophenyllcyclopentanecarboxamide
[00454] N-[4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-3- chlorophenyljcyclopentanecarboxamide was synthesized in a manner similar to Example 11 + Coupling, wherein cyclopentanecarbonyl chloride replaced 3- dimethylaminopropionic acid chloride. IH NMR (400 MHz, d6-DMSO): 13.33 (s, br, IH), 10.30 (s, IH), 8.23 (s, IH), 7.99 (s, IH), 7.85 (m, 2H), 7.61 (s, 2H), 2.81 (m, IH), 1.88 (m, 2H), 1.71 (m, 4H), 1.57 (m, 2H). MS (EI) for C22 H17 Br C1 N3 03: 488 (MH+).
(Compound 279) N-[4-(8-bromo-4-oxo-3,4-dihydro[llbenzofuro[3,2-dlpyrimidin-2-yl)-3- chlorophenyl] -4-chlorobenzamide [00455] N-[4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-3- chlorophenyl] -4-chlorobenzamide was synthesized in a manner similar to Example 11 + Coupling, wherein 4-chlorobenzoyl chloride replaced 3- dimethylaminopropionic acid chloride. IH NMR (400 MHz, d6-DMSO): 10.67 (s, IH), 8.21 (s, IH), 8.11 (s, IH), 8.03 (d, 2H), 7.84 (m, 3H), 7.67 (m, 3H). MS (EI) for C23 H12 Br C12 N3 03: 530 (MH+). (Compound 280)
N-[4-(8-bromo-4-oxo-3,4-dihydro[llbenzofuro[3,2-dlpyrimidin-2-yl)-3- chlorophenyl] -3 -chlorobenzamide
[00456] N-[4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-3- chlorophenyl] -3 -chlorobenzamide was synthesized in a manner similar to Example 11 + Coupling, wherein 3-chlorobenzoly chloride replaced 3- dimethylaminopropionic acid chloride. IH NMR (400 MHz, d6-DMSO): 10.70 (s, IH), 8.22 (s, IH), 8.10 (s, IH), 8.06 (s, IH), 7.96 (d, IH), 7.84 (m, 3H), 7.70, (m, 2H), 7.61 (m, IH). MS (EI) for C23 H12 Br C12 N3 03: 530 (MH+).
(Compound 281) N-r4-(8-bromo-4-oxo-3,4-dihvdrorilbenzofuror3,2-dlpyrimidin-2-yl)-3- chlorophenyl] -3 -(methyloxy)benzamide
[00457] N-[4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-3- chlorophenyl]-3-(methyloxy)benzamide was synthesized in a manner similar to Example 11 + Coupling, wherein 3-methoxybenzoyl chloride replaced 3- dimethylaminopropionic acid chloride. IH NMR (400 MHz, d6-DMSO): 13.39 (s,
IH), 10.62 (s, IH), 8.26 (s, IH), 8.14 (s, IH), 7.88 (m, 3H), 7.70 (d, IH), 7.58 (d, IH), 7.51 (m, 2H), 7.21 (d, IH), 3.86 (s, 3H). MS (EI) for C24 H15 Br Cl N3 04: 526 (MH+).
(Compound 285) N-r4-(8-bromo-4-oxo-3,4-dihvdrorilbenzofuror3,2-dlpyrimidin-2-yl)-3- chlorophenyll-4-(methyloxy)benzamide
[00458] N-[4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-3- chlorophenyl]-4-(methyloxy)benzamide was synthesized in a manner similar to Example 11 + Coupling, wherein 4-methoxybenzoly chloride replaced 3- dimethylaminopropionic acid chloride. IH NMR (400 MHz, d6-DMSO): 13.36 (s, br, IH), 10.46 (s, IH), 8.24 (s, IH), 8.13 (s, IH), 8.01 (d, 2H), 7.87 (m, 3H), 7.67 (d, IH), 7.11 (d, 2H), 3.87 (s, 3H). MS (EI) for C24 H15 Br Cl N3 04: 526 (MH+). (Compound 319)
N-[4-(8-bromo-4-oxo-3,4-dihydro[llbenzofuro[3,2-dlpyrimidin-2-yl)-3- chlorophenvH-2-(methyloxy)acetamide
[00459] N-[4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-3- chlorophenyl]-2-(methyloxy)acetamide was synthesized in a manner similar to Example 11 + Coupling, wherein methoxyacetyle chloride replaced 3- dimethylaminopropionic acid chloride. IH NMR (400 MHz, d6-DMSO): 10.21 (s, IH), 8.23 (s, IH), 8.03 (s, IH), 7.85 (m, 2H), 7.75 (dd, IH), 7.63 (d, IH), 4.07 (s, 2H), 3.40 (s, 3H). MS (EI) for C19 H13 Br C1 N3 04: 464 (MH+). (Compound 320) methyl [4-(8-bromo-4-oxo-3,4-dihydro[llbenzofuro[3,2-dlpyrimidin-2-yl)-3- chlorophenyl] carbamate
[00460] methyl [4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2- yl)-3-chlorophenyl] carbamate was synthesized in a manner similar to Example 11 + Coupling, wherein methyl chloroformate replaced 3-dimethylaminopropionic acid chloride. IH NMR (400 MHz, d6-DMSO): 13.30 (s, br, IH), 10.16 (s, IH), 8.24 (s, IH), 7.86 (m, 2H), 7.76 (s, IH), 7.62 (d, IH), 7.52 (dd, IH), 3.72 (s, 3H). MS (EI) for C18 HI l Br C1 N3 04: 450 (MH+).
(Compound 321) N-[4-(8-bromo-4-oxo-3,4-dihydro[llbenzofuro[3,2-dlpyrimidin-2-yl)-3- chlorophenyl]furan-2-carboxamide [00461] N-[4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-3- chlorophenyl]furan-2-carboxamide was synthesized in a manner similar to Example
11 + Coupling, wherein 3-chlorobenzoyl chloride replaced 3- dimethylaminopropionic acid chloride. IH NMR (400 MHz, d6-DMSO): 13.33 (s, br,
IH), 10.59 (s, IH), 8.24 (s, IH), 8.11 (s, IH), 8.01 (s, IH), 7.87 (m, 3H), 7.67 (d, IH),
7.43 (d, IH), 6.77 (s, IH). MS (EI) for C21 Hl 1 Br Cl N3 04: 486 (MH+).
(Compound 322)
N-r4-(8-bromo-4-oxo-3,4-dihvdrorilbenzofuror3,2-dlpyrimidin-2-yl)-3- chlorophenyl] -2-pyridin-3 -ylacetamide
[00462] N-[4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-3- chlorophenyl] -2 -pyridin-3 -ylacetamide was synthesized in a manner similar to
Example 11 + Coupling, wherein 2-(pyridin-3-yl)acetic acid replaced 3- dimethylaminopropionic acid. IH NMR (400 MHz, d6-DMSO): 10.67 (s, IH), 8.54 (s, IH), 8.48 (d, IH), 8.22 (d, IH), 7.94 (s, IH), 7.80 (m, 3H), 7.60 (m, 2H), 7.39 (m, IH), 3.78 (s, 2H). MS (EI) for C23 H14 Br Cl N4 03: 511 (MH+).
(Compound 323) N-r4-(8-bromo-4-oxo-3,4-dihvdrorilbenzofuror3,2-dlpyrimidin-2-yl)-3- chlorophenyllpyrazine-2-carboxamide
[00463] N-[4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-3- chlorophenyl]pyrazine-2-carboxamide was synthesized in a manner similar to Example 11 + Coupling, wherein pyrazine-2-carboxylic acid replaced 3- dimethylaminopropionic acid. IH NMR (400 MHz, d6-DMSO): 13.40 (s, br, IH), 11.21 (s, IH), 9.38 (s, IH), 8.98 (s, IH), 8.86 (s, IH), 8.26 (dd, 2H), 8.04 (dd, IH), 77.86 (m, 2H), 7.71 (d, IH). MS (EI) for C21 HI l Br C1 N5 03: 498 (MH+).
(Compound 324)
N-r4-(8-bromo-4-oxo-3,4-dihvdrorilbenzofuror3,2-dlpyrimidin-2-yl)-3- chlorophenyllisoxazole-5-carboxamide
[00464] N-[4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-3- chlorophenyl]isoxazole-5-carboxamide was synthesized in a manner similar to Example 11 + Coupling, wherein isoxazole-5-carboxylic acid replaced 3- dimethylaminopropionic acid. IH NMR (400 MHz, d6-DMSO): 11.16 (s, IH), 8.89 (s, IH), 8.22 (s, IH), 8.09 (s, IH), 7.84 (m, 3H), 7.70 (d, IH), 7.35 (s, IH). MS (EI) for C20 HlO Br Cl N4 04: 487 (MH+).
(Compound 334) methyl 6-({[4-(8-bromo-4-oxo-3,4-dihydro[llbenzofuro[3,2-dlpyrimidin-2-yl)-3- chlorophenyll amino I carbonvDpyridine-3 -carboxylate [00465] methyl 6-({[4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin- 2-yl)-3 -chlorophenyl] amino }carbonyl)pyridine-3 -carboxylate was synthesized in a manner similar to Example 11 + Coupling, wherein 5-(methoxycarbonyl)picolinic acid replaced 3 -dimethylaminopropionic acid. IH NMR (400 MHz, d6-DMSO): 11.21 (s, IH), 9.22 (s, IH), 8.58 (dd, IH), 8.33(d, IH), 8.27 (s, IH), 8.19 (s, IH), 8.03 (dd, IH), 7.81 (m, 2H), 7.67 (d, IH), 3.97 (s, 3H). MS (EI) for C24 H14 Br C1 N4 05: 555 (MH+). (Compound 335)
N-[4-(8-bromo-4-oxo-3,4-dihydro[llbenzofuro[3,2-dlpyrimidin-2-yl)-3- chlorophenyll-2-(methyloxy)pyridine-4-carboxamide
[00466] N-[4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-3- chlorophenyl]-2-(methyloxy)pyridine-4-carboxamide was synthesized in a manner similar to Example 11 + Coupling, wherein 2-methoxyisonicotinic acid replaced 3- dimethylaminopropionic acid. IH NMR (400 MHz, d6-DMSO): 13.39 (s, br, IH), 10.81 (s, IH), 8.40 (d, IH), 8.25 (s, IH), 8.12 (s, IH), 7.88 (m, 3H), 7.71 (d, IH), 7.48 (dd, IH), 7.36 (s, IH), 3.95 (s, 3H). MS (EI) for C23 H14 Br Cl N4 04: 527 (MH+). (Compound 336)
5-bromo-N-[4-(8-bromo-4-oxo-3,4-dihydro[llbenzofuro[3,2-dlpyrimidin-2-yl)-3- chlorophenyllpyridine-2-carboxamide
[00467] 5-bromo-N-[4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin- 2-yl)-3-chlorophenyl]pyridine-2-carboxamide was synthesized in a manner similar to Example 11 + Coupling, wherein 5-bromopicolinic acid replaced 3- dimethylaminopropionic acid. IH NMR (400 MHz, d6-DMSO): 13.36 (s, br, IH), 11.16 (s, IH), 8.91 (s, IH), 8.37 (dd, IH), 8.27 (dd, 2H), 8.13 (d, IH), 8.05 (dd, IH), 7.87 (m, 2H), 7.70 (d, IH). MS (EI) for C22 Hl 1 Br2 Cl N4 03: 576 (MH+).
(Compound 339) N-[4-(8-bromo-4-oxo-3,4-dihydro[llbenzofuro[3,2-dlpyrimidin-2-yl)-3- chlorophenyl] -5 -chloropyridine-2-carboxamide [00468] N-[4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-3- chlorophenyl] -5 -chloropyridine-2-carboxamide was synthesized in a manner similar to Example 11 + Coupling, wherein 5-chloropicolinic acid replaced 3- dimethylaminopropionic acid. IH NMR (400 MHz, d6-DMSO): 13.37 (s, br, IH),
11.16 (s, IH), 8.84 (s, IH), 8.30 (s, IH), 8.23 (m, 3H), 8.06 (d, IH), 7.87 (m, 2H),
7.70 (d, IH). MS (EI) for C22 Hl 1 Br C12 N4 03: 531 (MH+).
(Compound 373)
N-r4-(8-bromo-4-oxo-3,4-dihvdrorilbenzofuror3,2-dlpyrimidin-2-yl)-3- chlorophenyll-l,3-oxazole-2-carboxamide
[00469] N-[4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-3- chlorophenyl]-l,3-oxazole-2-carboxamide was synthesized in a manner similar to
Example 11 + Coupling, wherein isoxazole-2-carboxylic acid replaced 3- dimethylaminopropionic acid. IH NMR (400 MHz, d6-DMSO): 13.37 (s, IH), 11.28 (s, IH), 8.46 (s, IH), 8.23 (s, IH), 8.14 (s, IH), 7.93 (dd, IH), 7.85 (m, 2H), 7.68 (d, IH), 7.60 (s, IH). MS (EI) for C20 HlO Br Cl N4 04: 487 (MH+).
(Compound 389) N-r4-(8-bromo-4-oxo-3,4-dihvdrorilbenzofuror3,2-dlpyrimidin-2-yl)-3- chlorophenyll - 1 -methyl- 1 H-imidazole-4-carboxamide
[00470] N-[4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-3- chlorophenyl]-l -methyl- 1 H-imidazole-4-carboxamide was synthesized in a manner similar to Example 11 + Coupling, wherein 1 -methyl- lH-imidazole-4-carboxylic acid replaced 3 -dimethylaminopropionic acid. IH NMR (400 MHz, d6-DMSO):
10.35 (s, IH), 8.25 (dd, 2H), 7.97 (dd, IH), 7.88 (m, 3H), 7.83 (s, IH), 7.63 (d, IH), 3.77 (s, 3H). MS (EI) for C21 H13 Br C1 N5 03: 500 (MH+).
(Compound 390)
N-r4-(8-bromo-4-oxo-3,4-dihvdrorilbenzofuror3,2-dlpyrimidin-2-yl)-3- chlorophenyll - 1 -methyl- 1 H-imidazole-2-carboxamide
[00471] N-[4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-3- chlorophenyl]-l -methyl- 1 H-imidazole-2-carboxamide was synthesized in a manner similar to Example 11 + Coupling wherein 1 -methyl- lH-imidazole-2-carboxylic acid replaced 3 -dimethylaminopropionic acid. IH NMR (400 MHz, d6-DMSO): 13.33 (s, br, IH), 10.83 (s, IH), 8.23 (dd, 2H), 7.96 (dd, IH), 7.86 (m, 2H), 7.65 (d, IH), 7.52 (s, IH), 7.14 (s, IH), 4.02 (s, 3H). MS (EI) for C21 H13 Br Cl N5 03: 500 (MH+).
(Compound 391)
N-[4-(8-bromo-4-oxo-3,4-dihydro[llbenzofuro[3,2-dlpyrimidin-2-yl)-3- chlorophenyll - 1 -methyl- 1 H-pyrazole-3 -carboxamide [00472] N-[4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-3- chlorophenyl]-l -methyl- 1 H-pyrazole-3 -carboxamide was synthesized in a manner similar to Example 11 + Coupling wherein 1 -methyl- 1 H-pyrazole-3 -carboxylic acid replaced 3 -dimethylaminopropionic acid. IH NMR (400 MHz, d6-DMSO): 13.31 (s, br, IH), 10.56 (s, IH), 8.22 (d, 2H), 7.95 (d, IH), 7.90 (m, IH), 7.86 (m, 2H), 7.64 (d, IH), 6.81 (s, IH), 4.00 (s, 3H). MS (EI) for C21 H13 Br Cl N5 03: 500 (MH+). (Compound 135)
N-[4-(8-bromo-4-oxo-3,4-dihydro[llbenzofuro[3,2-dlpyrimidin-2-yl)-3- chlorophenyll -N ' 3 ' ,N ' 3 ' -dimethyl-beta-alaninamide
[00473] To a solution of 3-dimethylaminopropionic acid (108 mg, 0.7 mmol) in dichloromethane (5 mL) was added oxalyl chloride (245 μL, 2.8 mmol) followed by catalytic amount (2 drops) of dimethylformamide. The reaction mixture was stirred at room temperature for 1 hour. The solvent was concentrated in vacuo to give solid 3- dimethylaminopropionic acid chloride. The resulting product was used without further purification. A solution of 2-(4-amino-2-chlorophenyl)-8- bromo[l]benzofuro[3,2-d]pyrimidin-4(3H)-one (200 mg, 0.47 mmol) in pyridine (3 mL) was added to a suspension of 3-dimethylaminopropionic acid chloride (0.7 mmol) in dichloromethane (2 mL). The reaction was stirred at room temperature for 15 minutes. The solvent was concentrated under reduced pressure and the residue was taken up in 3 mL of dimethylformamide. Purification by preparative HPLC (reverse-phase, acetonitrile/water with 0.1% formic acid) gave 23 mg (10%) of N-[4- (8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-3-chlorophenyl]- N'3',N'3'-dimethyl-beta-alaninamide. IH NMR (400 MHz, d6-DMSO): 10.48 (s, IH), 8.23 (s, IH), 7.97 (s, IH), 7.85 (m, 2H), 7.60 (m, 2H), 2.62 (m, 2H), 2.53 (m, 2H), 2.22 (s, 6H). MS (EI) for C21 Hi8 Br Cl N4 O3: 491 (MH+). (Compound 218)
N-[4-(8-bromo-4-oxo-3,4-dihydro[llbenzofuro[3,2-dlpyrimidin-2-yl)-3- chlorophenyll-2-chloro-6-methylpyridine-4-carboxamide [00474] N-[4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-3- chlorophenyl]-2-chloro-6-methylpyridine-4-carboxamide was synthesized in a manner similar to Example 11 + coupling, wherein 3-dimethylaminopropionic acid was substituted with 2-chloro-6-methyl isonicotinic acid. Purification by preparative
HPLC (reverse-phase, acetonitrile/water with 0.1% formic acid), followed by concentration in vacuo and lyophilization afforded the title compound (27.9 mg).
NMR (400 MHz, d6-DMSO): 13.36 (s, IH), 10.88 (s, IH), 8.25 (d, IH), 8.09 (s, IH), 7.83-7.90 (m, 3H), 7.78 (s, IH), 7.73 (s, IH), 7.71 (s, IH), 2.58 (s, 3H). MS (EI) for
C23Hi3BrClN4O3: 544 (MH+). (Compound 275)
N-[4-(8-bromo-4-oxo-3,4-dihydro[llbenzofuro[3,2-dlpyrimidin-2-yl)-3- chlorophenyll -3 -chloropyridine-4-carboxamide
[00475] N-[4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-3- chlorophenyl] -3 -chloropyridine-4-carboxamide was synthesized in a manner similar to Example 11 + coupling, wherein 3-dimethylaminopropionic acid was substituted with 3-chloroisonicotinic acid. Purification by preparative HPLC (reverse-phase, acetonitrile/water with 0.1% formic acid), followed by concentration in vacuo and lyophilization afforded the title compound (17.2 mg). NMR (400 MHz, d6-DMSO): 13.39 (s, IH), 11.13 (s, IH), 8.84 (s, IH), 8.72 (d, IH), 8.24 (s, IH), 8.04 (s, IH), 7.82-7.90 (m, 2H), 7.75 (d, IH), 7.72 (s, 2H). MS (EI) for C22HnBrCl2N4O3: 530 (MH+).
(Compound 276)
N-r4-(8-bromo-4-oxo-3,4-dihvdrorilbenzofuror3,2-dlpyrimidin-2-yl)-3- chlorophenyll-2-chloropyridine-4-carboxamide
[00476] N-[4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-3- chlorophenyl]-2-chloropyridine-4-carboxamide was synthesized in a manner similar to Example 11 + coupling, wherein 3-dimethylaminopropionic acid was substituted with 2-chloroisonicotinic acid. Purification by preparative HPLC (reverse-phase, acetonitrile/water with 0.1% formic acid), followed by concentration in vacuo and lyophilization afforded the title compound (11.3 mg). NMR (400 MHz, d6-DMSO): 13.39 (s, IH), 10.93 (s, IH), 8.67 (d, IH), 8.25 (s, IH), 8.11 (s, IH), 8.06 (s, IH), 7.83-7.93 (m, 4H), 7.73 (d, IH). MS (EI) for C22HnBrCl2N4O3: 530 (MH+).
(Compound 291) N-[4-(8-bromo-4-oxo-3,4-dihydro[llbenzofuro[3,2-dlpyrimidin-2-yl)-3- chlorophenyll -6-methylpyridine-3 -carboxamide
[00477] N-[4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-3- chlorophenyl]-6-methylpyridine-3 -carboxamide was synthesized in a manner similar to Example 11 + coupling, wherein 3-dimethylaminopropionic acid was substituted with 6-methylnicotinic acid. Purification by preparative HPLC (reverse-phase, acetonitrile/water with 0.1% formic acid), followed by concentration in vacuo and lyophilization afforded the title compound (37.0 mg). NMR (400 MHz, d6-DMSO): 13.39 (s, IH), 10.72 (s, IH), 9.04 (d, IH), 8.25-8.27 (m, IH), 8.24 (d, IH), 8.13 (d, IH), 7.84-7.91 (m, 3H), 7.71 (d, IH), 7.47 (d, IH), 2.58 (s, 3H). MS (EI) for C23Hi4BrClN4O3: 509 (MH+).
(Compound 292)
N-r4-(8-bromo-4-oxo-3,4-dihvdrorilbenzofuror3,2-dlpyrimidin-2-yl)-3- chlorophenvHpyridine-3-carboxamide
[00478] N-[4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-3- chlorophenyl]pyridine-3-carboxamide was synthesized in a manner similar to Example 11 + coupling, wherein 3-dimethylaminopropionic acid was substituted with nicotinic acid. Purification by preparative HPLC (reverse-phase, acetonitrile/water with 0.1% formic acid), followed by concentration in vacuo and lyophilization afforded the title compound (7.5 mg). NMR (400 MHz, d6-DMSO): 10.83 (s, IH), 9.16 (d, IH), 8.80-8.82 (m, IH), 8.33-8.37 (m, IH), 8.22 (d, IH), 8.12 (d, IH), 7.95 (s, IH), 7.80-7.88 (m, 3H), 7.69 (d, IH), 7.60-7.64 (m, IH). MS (EI) for C22Hi2BrClN4O3: 495 (MH+). (Compound 293)
N-[4-(8-bromo-4-oxo-3,4-dihydro[llbenzofuro[3,2-dlpyrimidin-2-yl)-3- chlorophenyllpyridine-2-carboxamide
[00479] N-[4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-3- chlorophenyl]pyridine-2-carboxamide was synthesized in a manner similar to Example 11 + coupling, wherein 3-dimethylaminopropionic acid was substituted with picolinic acid. Purification by preparative HPLC (reverse-phase, acetonitrile/water with 0.1% formic acid), followed by concentration in vacuo and lyophilization afforded the title compound (33.8 mg). NMR (400 MHz, d6-DMSO): 13.36 (s, IH), 11.10 (s, IH), 8.79 (d, IH), 8.31 (d, IH), 8.16-8.26 (m, 2H), 8.03-8.15 (m, 2H), 7.81-7.90 (m, 2H), 7.66-7.76 (m, 2H). MS (EI) for C22Hi2BrClN4O3: 495 (MH+).
(Compound 294)
N-r4-(8-bromo-4-oxo-3,4-dihvdrorilbenzofuror3,2-dlpyrimidin-2-yl)-3- chlorophenvH-2,6-dichloropyridine-4-carboxamide [00480] N-[4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-3- chlorophenyl]-2,6-dichloropyridine-4-carboxamide was synthesized in a manner similar to Example 11 + coupling, wherein 3-dimethylaminopropionic acid was substituted with 2,6-dichloroisonicotinic acid. Purification by preparative HPLC (reverse-phase, acetonitrile/water with 0.1% formic acid), followed by concentration in vacuo and lyophilization afforded the title compound (10.4 mg). NMR (400 MHz, de-DMSO): 10.50 (s, IH), 8.12-8.25 (m, 2H), 7.80-7.91 (m, 2H), 7.67 (d, IH), 7.51- 7.56 (m, IH), 7.38 (t, IH), 7.14-7.18 (m, IH). MS (EI) for C22Hi0BrCl3N4O3: 564 (MH+).
(Compound 312)
N-[4-(8-bromo-4-oxo-3,4-dihydro[llbenzofuro[3,2-dlpyrimidin-2-yl)-3- chlorophenyl] -5 -methylpyrazine-2-carboxamide [00481] N-[4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-3- chlorophenyl] -5 -methylpyrazine-2-carboxamide was synthesized in a manner similar to Example 11 + coupling, wherein 3-dimethylaminopropionic acid was substituted with 5-methylpyrazine-2-carboxylic acid. Purification by preparative HPLC (reverse- phase, acetonitrile/water with 0.1% formic acid), followed by concentration in vacuo and lyophilization afforded the title compound (17.0 mg). NMR (400 MHz, d6- DMSO): 11.11 (s, IH), 9.21 (s, IH), 8.75 (s, IH), 8.17-8.28 (m, 2H), 8.01-8.06 (m, IH), 7.80-7.89 (m, IH), 7.69 (d, IH), 2.66 (s, 3H). MS (EI) for C22Hi3BrClN5O3: 510 (MH+).
(Compound 313)
N-r4-(8-bromo-4-oxo-3,4-dihvdrorilbenzofuror3,2-dlpyrimidin-2-yl)-3- chlorophenylltetrahydrofuran-3-carboxamide
[00482] N-[4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-3- chlorophenyl]tetrahydrofuran-3-carboxamide was synthesized in a manner similar to Example 11 + coupling, wherein 3-dimethylaminopropionic acid was substituted with tetrahydro-3-furoic acid. Purification by preparative HPLC (reverse-phase, acetonitrile/water with 0.1% formic acid), followed by concentration in vacuo and lyophilization afforded the title compound (35.0 mg). NMR (400 MHz, d6-DMSO): 13.30 (s, IH), 10.45 (s, IH), 8.24 (d, IH), 7.98 (d, IH), 7.82-7.89 (m, 2H), 7.57-7.66 (m, 2H), 3.96 (t, IH), 3.69-3.83 (m, 3H), 3.14-3.24 (m, IH), 2.05-2.16 (m, 2H). MS (EI) for C2IHi5BrClN3O4: 488 (MH+). (Compound 328)
N-[4-(8-bromo-4-oxo-3,4-dihydro[llbenzofuro[3,2-dlpyrimidin-2-yl)-3- chlorophenyl1pyrimidine-5-carboxamide [00483] N-[4-(8-bromo^-oxo-3,4-dihydro[l]benzofiuO[3,2-d]pyrimidin-2-yl)-3- chlorophenyl]pyrimidine-5-carboxamide was synthesized in a manner similar to Example 11 + coupling, wherein 3-dimethylaminopropionic acid was substituted with 5-pyrimidine carboxylic acid. Purification by preparative HPLC (reverse-phase, acetonitrile/water with 0.1% formic acid), followed by concentration in vacuo and lyophilization afforded the title compound (9.9 mg). NMR (400 MHz, d6-DMSO): 13.41 (s, IH), 10.96 (s, IH), 9.41 (s, IH), 9.36 (s, 2H), 8.25 (d, IH), 8.11 (d, IH), 7.83-7.91 (m, 3H), 7.73 (d, IH). MS (EI) for C2IHnBrClN5O3: 496 (MH+).
(Compound 341) N-r4-(8-bromo-4-oxo-3,4-dihvdrorilbenzofuror3,2-dlpyrimidin-2-yl)-3- chlorophenyl]furan-3-carboxamide
[00484] N-[4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-3- chlorophenyl]furan-3-carboxamide was synthesized in a manner similar to Example 11 + coupling, wherein 3-dimethylaminopropionic acid was substituted with 3-furoic acid. Purification by preparative HPLC (reverse-phase, acetonitrile/water with 0.1% formic acid), followed by concentration in vacuo and lyophilization afforded the title compound (10.0 mg). NMR (400 MHz, d6-DMSO): 10.28 (s, IH), 8.46 (s, IH), 8.19-8.23 (m, IH), 8.06 (d, IH), 7.78-7.88 (m, 4H), 7.76 (d, IH), 7.04 (s, IH). MS (EI) for C2IHnBrClN3O4: 484 (MH+). (Compound 343)
N-[4-(8-bromo-4-oxo-3,4-dihydro[llbenzofuro[3,2-dlpyrimidin-2-yl)-3- chlorophenyll -6-(methyloxy)pyridine-3 -carboxamide
[00485] N-[4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-3- chlorophenyl]-6-(methyloxy)pyridine-3 -carboxamide was synthesized in a manner similar to Example 11 + coupling, wherein 3-dimethylaminopropionic acid was substituted with 6-methoxynicotinic acid. Purification by preparative HPLC (reverse- phase, acetonitrile/water with 0.1% formic acid), followed by concentration in vacuo and lyophilization afforded the title compound (36.8 mg). NMR (400 MHz, d6- DMSO): 10.61 (s, IH), 8.82 (d, IH), 8.28(d, IH), 8.20 (d, IH), 8.10 (s, IH), 7.78- 7.85 (m, 3H), 7.65-7.70 (m, IH), 6.98 (d, IH), 3.95 (s, 3H). MS (EI) for C23Hi4BrClN4O4: 525 (MH+). (Compound 396)
N-[4-(8-bromo-4-oxo-3,4-dihydro[llbenzofuro[3,2-dlpyrimidin-2-yl)-3- chlorophenyll-6-(lH-pyrazol-l-yl)pyridine-3-carboxamide
[00486] N-[4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-3- chlorophenyl]-6-(lH-pyrazol-l-yl)pyridine-3-carboxamide was synthesized in a manner similar to Example 11 + coupling, wherein 3-dimethylaminopropionic acid was substituted with 6-(lH-pyrazol-l-yl) nicotinic acid. Purification by preparative HPLC (reverse-phase, acetonitrile/water with 0.1% formic acid), followed by concentration in vacuo and lyophilization afforded the title compound (6.0 mg). NMR (400 MHz, d6-DMSO): 10.82 (s, IH), 9.07 (d, IH), 8.74(d, IH), 8.54-8.58 (m, IH), 8.24 (d, IH), 8.08-8.15 (m, 2H), 7.93 (d, IH), 7.81-7.89 (m, 3H), 7.71 (d, IH), 6.65-6.68 (m, IH). MS (EI) for C25Hi4BrClN6O3: 561 (MH+).
(Compound 397)
N-r4-(8-bromo-4-oxo-3,4-dihvdrorilbenzofuror3,2-dlpyrimidin-2-yl)-3- chlorophenyllquinoxaline-2-carboxamide
[00487] N-[4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-3- chlorophenyl]quinoxaline-2-carboxamide was synthesized in a manner similar to Example 11 + coupling, wherein 3-dimethylaminopropionic acid was substituted with 2-quinoxaline carboxylic acid. Purification by preparative HPLC (reverse-phase, acetonitrile/water with 0.1% formic acid), followed by concentration in vacuo and lyophilization afforded the title compound (17.0 mg). NMR (400 MHz, d6-DMSO): 11.28 (s, IH), 9.59 (s, IH), 8.31-8.37 (m, 2H), 8.23-8.29 (m, 2H), 8.03-8.14 (m, 3H), 7.82-7.91 (m, 3H), 7.73-7.77 (m, IH). MS (EI) for C25Hi3BrClN5O3: 546 (MH+).
(Compound 405) N-[4-(8-bromo-4-oxo-3,4-dihydro[llbenzofuro[3,2-dlpyrimidin-2-yl)-3- chlorophenvH-l,8-naphthyridine-2-carboxamide
[00488] N-[4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-3- chlorophenyl]-l,8-naphthyridine-2-carboxamide was synthesized in a manner similar to Example 11 + coupling, wherein 3-dimethylaminopropionic acid was substituted with l,8-naphthyridine-2-carboxylic acid. Purification by preparative HPLC (reverse- phase, acetonitrile/water with 0.1% formic acid), followed by concentration in vacuo and lyophilization afforded the title compound (9.7 mg). NMR (400 MHz, d6- DMSO): 11.25 (s, IH), 9.27-9.30 (m, IH), 8.79 (d, IH), 8.63-8.69 (m, IH), 8.39 (d, 2H), 8.33 (s, IH), 8.24 (d, 3H), 8.05-8.12 (m, IH), 7.77-7.87 (m, 3H), 7.70 (d, IH), 6.65 (s, IH). MS (EI) for C25Hi3BrClN5O3: 546 (MH+).
(Compound 406)
N-r4-(8-bromo-4-oxo-3,4-dihvdrorilbenzofuror3,2-dlpyrimidin-2-yl)-3- chlorophenyll-l,2,3-thiadiazole-4-carboxamide
[00489] N-[4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-3- chlorophenyl]-l,2,3-thiadiazole-4-carboxamide was synthesized in a manner similar to Example 11 + coupling, wherein 3-dimethylaminopropionic acid was substituted with l,2,3-thiadiazole-4-carboxylic acid. Purification by preparative HPLC (reverse- phase, acetonitrile/water with 0.1% formic acid), followed by concentration in vacuo and lyophilization afforded the title compound (9.7 mg). NMR (400 MHz, d6- DMSO): 11.29 (s, IH), 9.84 (s, IH), 8.13-8.17 (m, 2H), 7.89-7.94 (m, IH), 7.73-7.80 (m, 2H), 7.63 (d, IH), 6.57 (s, IH). MS (EI) for Ci9H9BrClN5O3S: 546 (MH+).
(Compound 362) N-r4-(8-bromo-4-oxo-3,4-dihvdrorilbenzofuror3,2-dlpyrimidin-2-yl)-3- chlorophenyl] -5 -methylisoxazole-3 -carboxamide
[00490] N-[4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-3- chlorophenyl] -5 -methylisoxazole-3 -carboxamide was synthesized in a manner similar to Example 11 + coupling, wherein 3-dimethylaminopropionic acid was substituted with 5 -methylisoxazole-3 -carboxylic acid. Purification by preparative HPLC
(reverse-phase, acetonitrile/water with 0.1% formic acid), followed by concentration in vacuo and lyophilization afforded the title compound. NMR (400 MHz, d6- DMSO): 11.07 (s, IH), 8.25 (s, IH), 8.12 (s, IH), 7.91 (m, 3H), 7.70 (d, IH), 6.72 (s, IH), 2.53 (s, 3H). MS (EI) for C2IHi2BrClN4O4: 500 (MH+). (Compound 363)
N-[4-(8-bromo-4-oxo-3,4-dihydro[llbenzofuro[3,2-dlpyrimidin-2-yl)-3- chlorophenyllisoxazole-3-carboxamide
[00491] N-[4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-3- chlorophenyl]isoxazole-3-carboxamide was synthesized in a manner similar to Example 11 + coupling, wherein 3-dimethylaminopropionic acid was substituted with isoxazole-3 -carboxylic acid. Purification by preparative HPLC (reverse-phase, acetonitrile/water with 0.1% formic acid), followed by concentration in vacuo and lyophilization afforded the title compound. NMR (400 MHz, d6-DMSO): 11.16 (s, IH), 9.22 (d, IH), 8.25 (s, IH), 8.12 (s, IH), 7.87 (m, 3H), 7.71 (d, IH), 7.09 (d, IH). MS (EI) for C20Hi0BrClN4O4: 486 (MH+).
(Compound 374)
N-r4-(8-bromo-4-oxo-3,4-dihvdrorilbenzofuror3,2-dlpyrimidin-2-yl)-3- chlorophenyl1-l,3-oxazole-5-carboxamide
[00492] N-[4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-3- chlorophenyl]-l,3-oxazole-5-carboxamide was synthesized in a manner similar to
Example 11 + coupling, wherein 3-dimethylaminopropionic acid was substituted with oxazole-5-carboxylic acid. Purification by preparative HPLC (reverse-phase, acetonitrile/water with 0.1% formic acid), followed by concentration in vacuo and lyophilization afforded the title compound. NMR (400 MHz, d6-DMSO): 13.38 (br s, IH), 10.83 (s, IH), 8.73 (s, IH), 8.25 (d, IH), 8.08 (m, 2H), 7.90 (m, 3H), 7.72 (d, IH). MS (EI) for C20Hi0BrClN4O4: 486 (MH+).
(Compound 395) 'N-r4-(8-bromo-4-oxo-3.4-dihvdrorilbenzofuror3.2-dlpyrimidin-2-vn-3- chlorophenyl1-lH-pyrrolo|"2,3-b]pyridine-5-carboxamide
[00493] 'N-[4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-3- chlorophenyl]-lH-pyrrolo[2,3-b]pyridine-5-carboxamide was synthesized in a manner similar to Example 11 + coupling, wherein 3-dimethylaminopropionic acid was substituted with lH-pyrrolo[2,3-b]pyridine-6-carboxylic acid. Purification by preparative HPLC (reverse-phase, acetonitrile/water with 0.1% formic acid), followed by concentration in vacuo and lyophilization afforded the title compound. C24Hi3BrClN5O3: 534 (MH+).
(Compound 62) N-r4-(8-bromo-4-oxo-3,4-dihvdrorilbenzofuror3,2-dlpyrimidin-2-yl)-3- methylphenyl] -N ' 2 ' ,N ' 2 ' -dimethylglycinamide
[00494] N-[4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-3- methylphenyl]-N'2',N'2' -dimethylglycinamide was synthesized in a manner similar to Example 11 + coupling, wherein 3-dimethylaminopropionic acid was substituted with N'N'-dimethylglycine, and 2-(4-amino-2-chlorophenyl)-8- bromo[l]benzofuro[3,2-d]pyrimidin-4(3H)-one was substituted with 2-(4-amino-2- methylphenyl)-8-bromo[l]benzofuro[3,2-d]pyrimidin-4(3H)-one. Purification by preparative HPLC (reverse-phase, acetonitrile/water with 0.1% formic acid), followed by concentration in vacuo and lyophilization afforded the title compound. NMR (400 MHz, de-DMSO): 8.26 (s, IH), 8.19 (s, IH), 7.81 (br s, 2H), 7.65 (br s, 2H), 7.49 (d, IH), 7.09 (br s, IH), 2.51 (br s, 1 IH). MS (EI) for C2IHi9BrN4O3: 457 (MH+).
(Compound 393)
'3-amino-N-r4-(8-bromo-4-oxo-3,4-dihvdrorilbenzofuror3,2-dlpyrimidin-2-yl)-3- chlorophenyll-lH-indazole-S-carboxamide
[00495] '3-amino-N-[4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin- 2-yl)-3-chlorophenyl]-lH-indazole-5-carboxamide was synthesized was synthesized in a manner similar to Example 11 + coupling wherein 3-dimethylaminopropionic acid was substituted with 2-fluoro-5-formylbenzonitrile. Subsequently, resulting crude N-(3-(8-bromo-4-oxo-3,4-dihydrobenzofuro[3,2-d]pyrimidin-2-yl)-4- chlorophenyl)-3-cyano-4-fluorobenzamide was dissolved in DMSO (2mL) and hydrazine hydrate (1 mL). The reaction mixture was heated to 110 0C for 16h in a sealed vessel. Upon cooling the mixture was concentrated in vacuo and dissolved in methanol. Formation of product was confirmed by LC/MS and the product was purified by preparatory HPLC (reverse-phase, acetonitrile/water with 0.1% formic acid) to yeild the title compound. 1H-NMR (400MHz, d6-DMSO): 11.78 (br s, IH), 10.54 (s, IH), 8.46 (s, IH), 8.24 (s, IH), 8.14 (s, IH), 7.83 (m, 4H), 7.67 (d, IH), 7.31 (d, IH), 5.64 (s, 2H). MS (EI) for C24Hi4BrClN6O3: 549 (MH+). EXAMPLE 12
Scheme 6A
Figure imgf000230_0001
2 N NaOH
Figure imgf000230_0002
wherein R3a is as defined in the disclosure above and R36 and R37 are described within the compounds within this example. 5-Bromo-3-(3-chloropropanamido)benzofuran-2-carboxamide 46 [00496] The title compound was synthesized in a manner similar to Example 1, wherein chloroacetyl chloride was substituted with chloropropanoyl chloride. After purification by flash chromatography (50:45:5 hexane/ethyl acetate/methanol), the title compound was obtained as an oil (459 mg), quantitative yield.
1H NMR (400 MHz, d6-DMSO) δ 10.26 (br s, IH), 8.14 (br s, IH), 8.06 (d, J= 2.5 Hz, 1 H), 7.92 (br s, IH), 7.64 (dd, J= 2.5, J= 9 Hz, IH), 7.58 (d, J= 9 Hz, 1 H), 3.92 (t, J= 6 Hz, IH), 3.76 (t, J= 6 Hz, IH), 2.98 (t, J= 6 Hz, IH), 2.72 (t, J= 6 Hz, IH); MS (ESI+) for Ci2Hi0BrClN2O3: 346(MH+). (Compound 105)
2-(2-Chloro-6-fluorophenyl)-8-cvclopropyirilbenzofuror3,2-άπpyrimidin-4(3H)-one [00497] To a solution of 5-bromo-3-(3-chloropropanamido)benzofuran-2- carboxamide (46, 106 mg, 0.307 mmol) in 10 mL anhydrous ethanol was added pyrrolidine (80 μL, 0.95 mmol). The reaction mixture was heated to 80 0C for 17 h, then concentrated to dryness under reduced pressure. The residual crude material was diluted with absolute ethanol (10 mL); 2 N NaOH (3 ml) was added, and the mixture was heated at 40 0C for 1 h. After purification by flash chromatography (90:8.5:1.5 dichloromethane/methanol/28% (w/w) ammonium hydroxide), the title compound was obtained as a solid (89 mg), 79% yield (over two steps). 1H NMR (400 MHz, CDCl3) δ 12.11 (br s, IH), 8.12 (d, J= 2.5 Hz, IH), 7.62 (dd, J= 2.5, J= 9 Hz, IH), 7.48 (d, J= 9 Hz, IH), 3.06 (m, 4H ), 2.82 (m, 4H), 1.96 (m, 4H); 13C NMR (100 MHz, CDCl3) δ 159.1, 155.5, 154.2, 143.3, 139.4, 132.5, 124.7, 124.4, 117.1, 114.6, 53.6, 52.8, 31.7, 23.8; MS (ESI+) for Ci6Hi6BrN3O2: 364 (MH+).
(Compound 164) 8-bromo-2- { r(3R)-3-hydroxypyrrolidin- 1 -yllmethyll \ 1 lbenzofuro[3 ,2-dlpyrimidin- 4(3HVone
[00498] 8-bromo-2- { [(3R)-3-hydroxypyrrolidin- 1 -yl]methyl} [ 1 ]benzofuro[3 ,2- d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 12, wherein chloropropanoyl chloride was substituted with chloroacetyl chloride and pyrrolidine was substituted with R-(+)-3-pyrrolidinol. Purification by flash chromatography afforded the title compound (53 mg, 46%). 1H NMR (400 MHz, d6-DMSO + D2O): 8.22 (s, IH), 7.81 (s, 2H), 4.21 (m, IH), 3.76(m, 2H), 2.86 (m, 2H), 2.61 (m, 2H), 2.08 (m, IH), 1.65 (m, IH); MS (EI) for Ci5Hi4BrN3O3: 421 :423(Bromine isoptope MH+).
(Compound 176)
8-bromo-2-[(2 -phenyl- lH-imidazol- 1 -vDmethyl] [ 1 lbenzofuro[3 ,2-d]pyrimidin-4(3H)- one
[00499] 8-bromo-2-[(2-phenyl- 1 H-imidazol- 1 -yl)methyl] [ 1 ]benzofuro [3 ,2- d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 12, wherein chloropropanoyl chloride was substituted with chloroacetyl chloride and pyrrolidine was substituted with 2-Phenylimidazole. Purification by preparative HPLC, followed by concentration in vacuo and lyophilization afforded the title compound (21mg, 8%). 1H NMR (400 MHz, d6-DMSO): 8.02 (s, IH), 7.82 (m, 2H), 7.65 (m, 2H), 7.44 (m, 4H), 7.07 (s, IH), 5.29 (s, 2H); MS (EI) for C20Hi3BrN4O2: 421 :423(Bromine isoptope MH+).
(Compound 378) 8-bromo-2-(((lR.4R)-5-methyl-2.5-diazabicvclor2.2.11heptan-2- yl)methyl)benzofuro|"3,2-d]pyrimidin-4(3H)-one
[00500] 8-bromo-2-(((lR,4R)-5-methyl-2,5-diazabicyclo[2.2.1]heptan-2- yl)methyl)benzofuro[3,2-d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 12, wherein chloropropanoyl chloride was substituted with chloroacetyl chloride and pyrrolidine was substituted with (lR,4R)-2-methyl-2,5- diazabicyclo [2.2.1] heptane and triethyl amine. (li?,4i?)-2-methyl-2,5- diazabicyclo [2.2.1] heptane was synthesized according to a procedure published in J. Org. Chem, 1990, vol. 55, 1684-1687. To a solution of the resulting 5-bromo-3-(2- ((lR,4R)-5-methyl-2,5-diazabicyclo[2.2.1]heptan-2-yl)acetamido)benzofuran-2- carboxamide (140 mg, 0.34 mmol) in ethanol (5 mL) was added an aqueous solution of 2N NaOH (5 mL) and the resulting mixture was heated to 50 0C for 4 hours. The reaction mixture was concentrated in vacuo and brought to pH 9 and extracted with chloroform (3 x 50 mL). The combined organic phases were washed with brine, dried over Na2SO4 and concentrated in vacuo. Purification by preparative HPLC afforded the title compound (38.4 mg, 29%). 1H NMR (400 MHz, d6-DMSO): 8.18 (m, IH), 7.80 (m, 2H), 3.72 (m, 2H), 3.40 (m, IH), 3.21 (m, IH), 2.75 (m, 3H), 2.56 (m, IH), 2.30 (s, 3H), 1.67 (m, 2H). MS (EI) for Ci7Hi7BrN4O2: 388.9 (MH+). (Compound 159)
8-bromo-2-(piperazin- 1 -ylmethyl)[ 11benzomro[3,2-d]pyrimidin-4(3H)-one (HCl salt) [00501] 8-bromo-2-(piperazin- 1 -ylmethyl)[ 1 ]benzofuro[3,2-d]pyrimidin-4(3H)- one was synthesized in a manner similar to Example 12, wherein chloropropanoyl chloride was substituted with chloroacetyl chloride and pyrrolidine was substituted with N-Boc-piperazine. The pure material was given by deprotection with 4N-HC1 in dioxane. 1H NMR (400 MHz, CD3OD): 8.25 (q, IH), 7.80 (dd, H), 7.70 (dd, IH), 3.91 (s, 2 H), 3.37 (br, 4 H), 3.25 (br, 4H); MS (EI) for Ci5Hi5BrN4O2: 363 (MH+).
(Compound 183) 8-bromo-2-r(3-hvdroxyazetidin-l-yl)methylirilbenzofuror3,2-dlpyrimidin-4(3H)-one [00502] 8-bromo-2-[(3-hydroxyazetidin-l-yl)methyl][l]benzofuro[3,2- d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 12, wherein chloropropanoyl chloride was substituted with chloroacetyl chloride and pyrrolidine was substituted with azetidin-3-ol. The crude product was purified by SiO2 flash chromatography to afford the desired product. 1H NMR (400 MHz, CD3OD): 8.2 (q, IH), 7.75 (dd, IH), 7.63 (dd, IH), 4.55 (q, IH), 4.15 (m, 2H), 4.03 (s, 2H), 3.55 (m, 2 H); MS (EI) for Ci4Hi2BrN3O3: 350 (MH+).
(Compound 187)
2-IY4-acetylpiperazin- 1 -yl)methyl"|-8-bromor 1 lbenzofuror3,2-dlpyrimidin-4(3H)-one [00503] 2-[(4-acetylpiperazin- 1 -yl)methyl]-8-bromo[ 1 ]benzofuro[3,2-d]pyrimidin- 4(3H)-one was synthesized in a manner similar to Example 12, wherein chloropropanoyl chloride was substituted with chloroacetyl chloride and pyrrolidine was substituted with N-Boc-piperazine. The pure material was given by deprotection with 4N-HC1 in dioxane and followed by acetylation with acetic anhydride in DIEA. 1H NMR (400 MHz, CD3OD): 8.23 (s, IH), 7.78 (d, IH), 7.68 (d, IH), 3.66 (m, 4H), 3.60 (m, 2H), 2.59(m, 4H), 2.10 (s, 3H); MS (EI) for Ci7Hi7BrN4O3: 405 (MH+).
(Compound 306)
8-bromo-2-{r3-(hvdroxymethyl)piperidin-l-yllmethvUrilbenzofuror3,2-dlpyrimidin- 4(3HVone [00504] 8-bromo-2-{[3-(hydroxymethyl)piperidin-l-yl]methyl} [l]benzofuro[3,2- d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 12, wherein chloropropanoyl chloride was substituted with chloroacetyl chloride and pyrrolidine was substituted with piperidin-4-ylmethanol. Purification by preparative HPLC (reverse-phase, acetonitrile/water with 0.01% ammonium acetate), followed by concentration in vacuo and lyophilization afforded the title compound as a solid (48% yield). 1H NMR (400 MHz, CD3OD): 8.21 (s, IH), 7.77 (d, IH), 7.66 (d, IH), 3.77 (d, 2H), 3.49 (m, IH), 3.40 (m, IH), 3.14 (d, IH), 3.03 (d, IH), 2.41 (t, IH), 2.20 (t, IH), 1.92 (m, IH), 1.76 (m, 3H), 1.1 (m, IH); MS (EI) for Ci7Hi8BrN3O3: 392 (MH+).
(Compound 367)
8-Fluoro-2-[(4-methylpiperazin- 1 -yDmethyl] [ 1 ]benzofuro[3 ,2-<i]pyrimidin-4(3H)-one [00505] The title compound was synthesized in a manner similar to Example 12, wherein 3-amino-5-bromobenzofuran-2-carboxamide was substituted with 3-amino-5- fluorobenzofuran-2-carboxamide, which in turn was synthesized in a manner similar to Example 1, wherein 5-bromo-2-hydroxybenzonitrile was substituted with 5-fluoro-
2-hydroxybenzonitrile. After purification by flash chromatography (91 :8:1 dichloromethane/methanol/28% (w/w) ammonium hydroxide), the title compound was obtained as a solid (89 mg), 17% yield. 1H NMR (400 MHz, 1 :1 CD3OD/CDC13) δ 7.75 (dd, IH), 7.66 (dd, IH), 7.39 (dt,
IH), 4.59 (s, 2H), 3.71 (s, 3H), 2.72 (m, 4H), 2.39 (s, 4H); MS (ESI+) for
Ci6Hi7FN4O2: 317 (MH+).
(Compound 230)
8-Iodo-2-|Y4-methylpiperazin- 1 -yDmethyl] [ 1 ]benzomro[3,2- J]pyrimidin-4(3H)-one [00506] The title compound was synthesized in a manner similar to Example 12, wherein 3 -(2-chloropropanamido)-5 -bromobenzofuran-2-carboxamide was substituted with 3-(2-chloroacetamido)-5-iodobenzofuran-2-carboxamide (5). After purification by flash chromatography (91 :8:1 dichloromethane/methanol/28% (w/w) ammonium hydroxide), the title compound was obtained as a solid (200 mg), 69% yield.
1H NMR (400 MHz, CD3OD ) δ 8.42 (d, IH), 7.95 (dd, IH), 7.57 (dd, IH), 3.76 (s, 2H), 3.51 (br, 3H ), 3.20 (m, 5H), 2.92 (s, 3H); MS (ESI+) for Ci6Hi6BrN3O2: 425 (MH+). Scheme 6B
Figure imgf000235_0001
(Compound 129) 8-Bromo-2-r2-( ( r4-(4-methylpiperazin- 1 - vDphenyllmethvU amino)ethyll [ 1 lbenzofuro [3 ,2-άπpyrimidin-4(3/-/)-one [00507] Intermediate 5-bromo-3-[3-[4-(4-methylpiperazin-l- yl)benzylamino]propanamido]benzofuran-2-carboxamide (3) was synthesized in a manner similar to Example 2, wherein 8-bromo-2-(chloromethyl)benzofuro[3,2- </]pyrimidin-4(3H)-one was substituted with 5-bromo-3-(3- chloropropanamido)benzofuran-2-carboxamide (46, prepared as described in Example 12). Compound 3 was not isolated pure, but directly treated with 2 N NaOH in ethanol, in a similar manner as described in Example 12. After purification by flash chromatography (88:10:2 dichloromethane/methanol/28% (w/w) ammonium hydroxide), the title compound was obtained as an impure oil (34 mg), 44% yield over two steps. Purification by preparative HPLC (reverse-phase, 0.1% TFA in acetonitrile/0.05% TFA in water), followed by concentration in vacuo and lyophilization afforded the title compound (22 mg, 28%) as a white solid. 1H NMR (400 MHz, CD3OD) δ 8.20 (d, J= 2.5 Hz, IH), 7.80 (dd, J= 2.5, J= 9 Hz, IH), 7.69 (d, J= 9 Hz, IH), 7.51 (d, J= 8 Hz, IH), 7.14 (d, J= 8 Hz, IH), 4.31 (s, 2H), 3.95 (m, 2H), 3.63 (m, 2H), 3.59 (t, J= 6 Hz, 2H), 3.26 (m, 2H), 3.22 (t, J= 6 Hz, 2H), 3.08 (m, 2H), 2.97 (s, 3H); MS (ESI+) for C24H26BrN5O2: 497 (MH+). EXAMPLE 13:
Scheme 7
Figure imgf000236_0001
toluene, 11O 0C
Figure imgf000236_0002
Figure imgf000236_0003
(Compound 106)
8-Bromo-2-[(4-methylpiperazin-l-yl)carbonyll[llbenzofuro[3,2-άπpyrimidin-4(3H)- one
[00508] To a solution of 3-amino-5-bromobenzofuran-2-carboxamide (213 mg, 0.835 mmol) in benzene (3 niL) and pyridine (0.2 rnL), methyl oxalyl chloride (315 mg, 2.59 mg) was added at room temperature. An equal volume Of CH2Cl2 (about 3 mL) was added. The mixture was heated to 50 0C in sealed tube for 3 h, then filtered through CeliteR and concentrated to dryness under reduced pressure. After purification by flash chromatography (90:9:1 dichloromethane/methanol/28% (w/w) ammonium hydroxide), intermediate compound 2a (methyl 8-bromo-4-oxo-3,4- dihydrobenzofuro[3,2-J]pyrimidine-2-carboxylate) and tautomer 2b (methyl 8- bromo-4-oxo- 1 ,4-dihydrobenzofuro [3 ,2- J]pyrimidine-2-carboxylate) were obtained as inseparable mixture (195 mg, 0.60 mmol; 72% yield), which could only be partially characterized analytically [MS (ESI+) for Ci2H7BrN2O4: 324 (MH+)]. To a solution of the obtained mixture of compounds 2a and 2b in anhydrous toluene (10 mL) was added jV-methylpiperazine (123 mg, 1.23 mmol). The reaction mixture was heated to 110 0C for 2 hours, cooled down and concentrated in vacuo. After purification by flash chromatography (88: 10:2 dichloromethane/methanol/28% (w/w) ammonium hydroxide), the title compound was obtained as a solid (126 mg), 54% yield. 1U NMR (400 MHz, CDCl3) δ 10.13 (br s, IH), 7.95 (d, J= 1.7 Hz, IH), 7.62 (dd, J= 2, J= 9 Hz, IH), 7.41 (d, J= 9 Hz, IH), 4.22 (m, 2H), 3.75 (m, 2H), 2.52 (m, 4H), 2.34 (s, 3H); 13C NMR (100 MHz, CDCl3) δ 158.9, 153.2, 132.5, 127.2, 124.3, 123.2, 120.6, 117.6, 114.1, 111.1, 55.5, 54.8, 46.8, 46.1, 44.1; MS (ESI+) for Ci6Hi5BrN4O3: 392 (MH+).
EXAMPLE 14:
Scheme 8
Figure imgf000237_0002
Figure imgf000237_0001
5 -Bromo-3 -(2-bromopropanamido)benzofuran-2-carboxamide (2)
[00509] The title compound was synthesized in a manner similar to Example 1, wherein chloroacetyl chloride was substituted with 2-bromopropanoyl chloride. After purification by flash chromatography (91 :8:1 dichloromethane/methanol/28% (w/w) ammonium hydroxide), the title compound was obtained as an oil (179 mg), quantitative yield.
1H NMR (400 MHz, d6-DMSO) δ 8.24 (br s, IH), 8.10 (m, IH), 7.94 (br s, IH), 7.65 (dd, J= 2.5, J= 9 Hz, IH), 7.59 (d, J= 9 Hz, IH), 5.00 (q, J= 6.5 Hz, IH), 1.82 (d, J= 6.5 Hz, 3H); MS (ESI+) for Ci2Hi0Br2N2O3: 391 (MH+).
(Compound 117) 8-Bromo-2-r 1 -(4-methylpiperazin- 1 -vQethyll [ 1 Ibenzofuroβ .2-άπpyrimidin-4(3HV one
[00510] The title compound was synthesized in a manner similar to Example 12 wherein pyrrolidine was substituted with JV-methylpiperazine and ethanol with N- methylpyrrolidinone (NMP). The reaction in the second step was run at 80 0C for 18 h. After purification by flash chromatography (95 :4.5 :0.5 to 90:9: 1 dichloromethane/methanol/28% (w/w) ammonium hydroxide), the title compound was obtained as a solid (41 mg), 51% (over two steps).
1U NMR (400 MHz, CDCl3, spiked with CD3OD) δ 8.24 (d, J= 2.5 Hz, IH), 7.71 (dd, J= 2.5, J= 9 Hz, IH), 7.56 (d, J= 9 Hz, IH), 3.72 (q, J= 7 Hz, IH), 3.59 (br s, 2H), 2.71 (m, 2H), 2.59 (m, 4 H), 2.34 (s, 3H), 1.50 (d, J= 7 Hz, 3H); 13C NMR (100 MHz, CDCl3 spiked with CD3OD) δ 159.8, 156.0, 153.4, 144.0, 133.0, 124.7, 124.4, 117.5, 114.7, 110.2, 62.9, 55.1, 45.8, 22.3, 14.3, MS (ESI+) for Ci7Hi9BrN4O2: 392 (MH+). EXAMPLE 15:
Scheme
— . .NHBoc
Figure imgf000238_0001
Figure imgf000238_0002
ferf-Butyl 2-(8-bromo-4-oxo-3,4-dihydrobenzofuro[3,2-(i]pyrimidin-2- ylamino)ethylcarbamate (2)
(Compound 309)
8-Bromo-2-r(2-hvdroxyethyl)aminoiπibenzofuror3,2-άπpyrimidin-4(3/-/)-one [00511] The title compound was synthesized in a manner similar to Example 15 wherein JV-methylpiperazine was substituted with ethanolamine, and ethanol with n- propanol. Purification by preparative HPLC (reverse-phase, 0.1% TFA in acetonitrile/0.05% TFA in water), followed by concentration in vacuo and lyophilization afforded the title compound (5 mg, 9%) as a white solid. FT-IR (solid): 3270, 3090, 2950, 1699, 1669, 1602, 1309, 1205, 1120, 1064 cm"1; 1H NMR (400 MHz, CD3OD ) δ 8.01 (d, IH), 7.72 (d, IH), 7.71 (d, IH), 6.57 (m, IH), 5.15 (br t, IH), 3.57 (dd, 2H), 2.85 (t, 2H); MS (ESI+) for Ci2Hi0BrN3O3: 325 (MH+). EXAMPLE 16:
(l^^-fert-Butyl S-ffS-bromo^-oxo-S^-dihydrobenzofurofS^-anpyrimidin-l- yl)methvH-2,5-diazabicyclor2.2.1 lheptane-2-carboxylate [00512] In a manner similar to Example 1, a solution of 8-bromo-2- (chloromethyl)benzofuro[3,2-J]pyrimidin-4(3H)-one (161 mg, 0.513 mmol), (15,4S)- tert-butyi 2,5-diazabicyclo[2.2.1]heptane-2-carboxylate (132 mg, 0.666 mmol) and diisopropylethylamine (150 μL, 0.84 mmol) in absolute ethanol (10 mL) was heated to 80 0C for 24 h. After cooling to room temperature, the reaction mixture was diluted with ethyl acetate and washed with aq. NaHCO3. The aqueous layer was further extracted with ethyl acetate and dichloromethane. The combined organic phases were washed with brine and dried over MgSO4. After purification by flash chromatography (91 :8:1 dichloromethane/methanol/28% (w/w) ammonium hydroxide), the title compound was obtained as an oil (162 mg), 58% yield. 1H NMR (400 MHz, 1 :1 CDCI3/CD3OD) δ 8.21 (d, J= 2.2 Hz, IH), 7.75 (dd, J= 2.5, J= 9 Hz, IH), 7.62 (d, J= 9 Hz, IH), 4.40 (d, J= 11 Hz, IH), 3.67 (d, J= 10 Hz, IH), 3.59 (d, J= H Hz, IH), 3.28 (t, J= 9 Hz, IH), 3.06 (t, J= 9 Hz, IH), 2.81 (dd, J= 10, J= 34 Hz, IH), 2.01 (d, J= 9 Hz, IH), 1.85 (t, J= 11 Hz, IH), 1.49 (s, 9H); MS (ESI+) for C2IH23BrN4O4: 476 (MH+).
(Compound 137) 8-Bromo-2-r(15.45)-2.5-diazabicvclor2.2.11hept-2-ylmethylirilbenzofuror3.2- άπpyrimidin-4(3H)-one
[00513] (15f,45)-fert-Butyl 5-((8-bromo-4-oxo-3,4-dihydrobenzofuro[3,2- <i]pyrimidin-2-yl)methyl)-2,5-diazabicyclo[2.2.1]heptane-2-carboxylate (157 mg, 0.33 mmol) was dissolved in CH2Cl2 (8 mL) and treated with TFA (1.5 mL) and triethylsilane (1 mL) at room temp for 4 h. After concentration under reduced pressure and azeotropic distillation with toluene, the residual crude mixture was purified by preparative HPLC (reverse-phase, 0.1% TFA in acetonitrile/0.05% TFA in water), followed by concentration in vacuo and lyophilization to afford the TFA salt of the title compound (149 mg) as a white solid, 92% yield. 1H NMR (400 MHz, CD3OD) δ 8.08 (d, J= 2.2 Hz, IH), 7.68 (dd, J= 2.5, J= 9 Hz, IH), 7.54 (d, J= 9 Hz, IH), 4.63 (d, J= 20 Hz, 2H), 4.56 (d, J= 20 Hz, 2H), 3.95 (d, J= 13 Hz, IH), 3.87 (d, J= 13 Hz, IH), 3.75 (m, IH), 3.60 (m, IH), 2.61 (d, J= 12 Hz, IH), 2.28 (d, J= 12 Hz, IH); 13C NMR (100 MHz, CD3OD) δ 166.2, 155.7, 153.6, 142.5, 139.2, 133.0, 124.0, 123.8, 117.3, 114.4, 63.3, 57.5, 57.0, 54.6, 33.7; MS (ESI+) for Ci6Hi5BrN4O2: 376 (MH+).
Scheme 9
Figure imgf000240_0001
tert-Butγl 8-azabicyclo[3.2.1"|octan-3-ylcarbamate (3) [00514] A 50 mL round bottom flask was charged with 8-benzyl-8- azabicyclo[3.2.1]octan-3-amine (202 mg, 0.61 mmol), DMAP (7 mg, 0.06 mmol), di- tert-butyldicarbonate (147 mg, 0.67 mmol), dichloromethane (10 mL) and DIEA (0.22 mL, 1.3 mmol). After stirring for 4 h at room temperature, the reaction mixture was concentrated to dryness under reduced pressure, without isolation of intermediate tert-butyi 8-benzyl-8-azabicyclo[3.2.1]octan-3-ylcarbamate (2). The obtained crude solid material was transferred in a Parr vessel and diluted with ethanol (10 mL) and acetic acid (1.5 mL). 10% (w/w) Pd/C was added (300 mg). The hydrogenation was carried out at 40 psi. The reaction was completed after 6 h (TLC). The mixture was filtered through Celite®. The obtained clear solution was concentrated in vacuo and azeotroped with toluene. After purification by flash chromatography (88:10:2 dichloromethane/methanol/28% (w/w) ammonium hydroxide), the title compound was obtained as an oil (106 mg), 77% yield over two steps. 1H NMR (400 MHz, CDCl3) δ 3.82 (br s, IH), 3.52 (br s, 2H), 2.09 (br s, 2H), 2.05 (m, 2H), 1.89 (m, 3H), 1.70 (d, J= 14 Hz, 2H), 1.44 (s, 9H); 13C NMR (100 MHz, CDCl3) δ 155.5, 53.6, 43.2, 37.9, 29.2, 28.6; MS (ESI+) for Ci2H22N2O2: 227 (MH+). ferf-Butyl 8-[(8-bromo-4-oxo-3,4-dihydrobenzofuro[3,2-(/|pyrimidin-2-yl)methyll-8- azabicyclo[3.2.1 loctan-3-ylcarbamate (4) [00515] The title compound was synthesized in a manner similar to Example 16 wherein (lS,4S)-tert-buty\ 2,5-diazabicyclo[2.2.1]heptane-2-carboxylate was substituted with tert-butyl 8-azabicyclo[3.2.1]octan-3-ylcarbamate (3). After purification by flash chromatography (91 :8:1 dichloromethane/methanol/28% (w/w) ammonium hydroxide), the title compound was obtained as an oil (76 mg), 71% yield. 1H NMR (400 MHz, CDCl3) δ 8.13 (d, J= 2.2 Hz, IH), 7.73 (dd, J= 2.5, J= 9 Hz, IH), 7.59 (d, J= 9 Hz, IH), 4.50 (br s, 2H), 4.42 (br s, 2H), 3.82 (t, J= 8 Hz, IH), 3.30 (m, IH), 2.97 (m, 2H), 2.61 (m, 2H), 2.26-2.34 (m, 4H), 1.79 (d, J= 14 Hz, 2H), 1.45 (s, 9H); 13C NMR (100 MHz, CDCl3) δ 156.6, 156.0, 155.6, 152.8, 143.9, 139.4, 132.9, 124.5, 117.4, 114.8, 59.9, 55.7, 53.7, 42.2, 37.2, 20.7, 26.5; MS (ESI+) for C23H27BrN4O4: 504 (MH+).
(Compound 131)
2-(r(li?.5^-3-Amino-8-azabicvclor3.2.11oct-8-yllmethyl|-8-bromorilbenzofuror3.2- <i]pyrimidin-4(3H)-one [00516] The title compound was synthesized in a manner similar to Example 16 and obtained as a solid (TFA salt, 75 mg), 96% yield.
1H NMR (400 MHz, CD3OD) δ 8.15 (d, J= 2.2 Hz, IH), 7.68 (dd, J= 2.5, J= 9 Hz, IH), 7.54 (d, J= 9 Hz, IH), 4.87 (br d, J= 6 Hz, IH), 3.86 (br s, IH), 3.66 (s, 2H), 3.22 (br s, 2H), 2.26 (m, 2H), 2.14 (m, 2H), 1.97 (m, 3H), 1.79 (d, J= 14 Hz, 2H), 1.45 (s, 9H); MS (ESI+) for Ci8Hi9BrN4O2: 404 (MH+).
(Compound 132)
2-{[(li?,561-3-amino-8-azabicyclo[3.2.11oct-8-yllmethyl|-8-bromo[llbenzofuro[3,2- άπpyrimidin-4(3/-/)-one [00517] The title compound was synthesized in a manner similar to Example 16, wherein (lS,4S)-tert-buty\ 2,5-diazabicyclo[2.2.1]heptane-2-carboxylate was substituted with (5)-octahydropyrrolo[l,2-α]pyrazine. After purification by flash chromatography (90:9:1 dichloromethane/methanol/28% (w/w) ammonium hydroxide), the title compound was obtained as an oil (54 mg), 42% yield. 1H NMR (400 MHz, CDCl3) δ 8.17 (d, J= 2.5 Hz, IH), 7.68 (dd, J= 2.5, J= 9 Hz, IH), 7.54 (d, J= 9 Hz, IH), 3.73 (d, J= 4 Hz, 2H), 3.09 (dd, J= 10, J= 18 Hz, 2H), 2.98 (d, J= 10 Hz, IH), 2.83 (d, J= 10 Hz, IH), 2.59 (m, IH), 2.36 (m, IH), 2.11-2.27 (m, 3H), 1.71-1.91 (m, 3H), 1.41 (m, IH), 1.13 (m, IH); 13C NMR (IOO MHZ, CDCl3) δ 156.0, 155.9, 153.0, 144.0, 139.4, 133.0, 124.6, 124.5, 117.5, 114.7, 62.8, 60.0, 58.5, 53.5, 53.0, 51.6, 27.7, 21.5; MS (ESI+) for Ci8Hi9BrN4O2: 404 (MH+). EXAMPLE 17:
Scheme 10
Figure imgf000242_0001
5 -Bromo-3 -(2-bromo-3 -methylbutanamido)benzofuran-2-carboxamide (2) [00518] To a solution of 2-bromo-3 -methyl butanoic acid (1.07 g, 5.91 mmol) in anhydrous CH2Cl2 (10 mL), a droplet of DMF and oxalyl chloride (1.5 mL, 17 mmol) were added at room temperature under nitrogen atmosphere. After stirring for 16 h, the reaction mixture was concentrated under reduced pressure and azeotroped with toluene. To the obtained residue, 3-amino-5-bromobenzofuran-2-carboxamide (1, 186 mg, 0.73 mmol) and chloroform were added. The mixture was heated to 40 0C for 3 h. The mixture was poured over aqueous saturated NaHCO3 (120 mL) and the organic phase was separated. The aqueous layer was further extracted with CHCl3 (2x 80 mL) . The combined organic layers were dried over MgSO4, then concentrated in vacuo. After purification by flash chromatography (91 :8:1 dichloromethane/methanol/28% (w/w) ammonium hydroxide), the title compound was obtained as an oil (198 mg), 65% yield. 1H NMR (400 MHz, CDCl3) δ 10.64 (br s, IH), 8.66 (d, J= 3 Hz, IH), 7.56 (dd, J= 3, J= 9 Hz, IH), 7.31 (d, J= 9 Hz, IH), 6.41 (br s, IH), 5.80 (br s, IH), 4.37 (d, J= 6.5 Hz, IH), 2.46 (sept, J= 6.5 Hz, IH), 1.14 (dd, J= 3, J= 6.5 Hz, IH); 13C NMR (100 MHz, CDCl3) δ 167.3, 162.6, 152.4, 133.0, 131.8, 129.7, 127.9, 123.4, 116.7, 113.4, 59.5, 33.1, 20.9, 19.5; MS (ESI+) for Ci4Hi4Br2N2O3: 419 (MH+). (Compound 153)
8-Bromo-2-(2 -methyl- 1 -pyrrolidin- 1 -ylpropyl) [ 1 Ibenzofuro [3 ,2-άπpyrimidin-4(3H)- one
[00519] 5 -Bromo-3 -(2-bromo-3 -methylbutanamido)benzofuran-2-carboxamide (2, 85 mg, 0.203 mmol), pyrrolidine (2 rnL) and tetrabutylammonium iodide (58 mg, 0.16 mmol) were placed in a test tube and heated to 120 0C for 15 min in a CEM- Discover microwave reactor. The reaction mixture, containing 5-bromo-3-(3-methyl- 2-(pyrrolidin-l-yl)butanamido)benzofuran-2-carboxamide (3), was concentrated under reduced pressure and azeotroped with acetonitrile. The residual material was diluted with absolute ethanol (5 mL). 2 N NaOH (1 mL) was added, the mixture was heated to 130 0C for 30 min in a CEM -Discover microwave reactor. After concentration under reduced pressure, the crude reaction mixture was partitioned between water and ethyl acetate. The aqueous phase was further extracted with ethyl acetate and with chloroform (7 x 50 mL). The combined organic layers were dried over MgSO4, then concentrated in vacuo. After purification by flash chromatography (91 :8:1 dichloromethane/methanol/28% (w/w) ammonium hydroxide), the title compound was obtained as a solid (71 mg), 40% yield over two steps. 1H NMR (400 MHz, CD3OD) δ 8.17 (m, IH), 7.70 (dd, J= 2.2, J= 9 Hz, IH), 7.61 (dd, J= 1, J= 9 Hz, IH), 3.46 (m, 5H), 3.12 (s, 2H), 2.14 (m, 5H), 1.44 (s, 6H); MS (ESI+) for Ci8H20BrN3O2: 391 (MH+).
Scheme 11
Figure imgf000244_0001
2-Bromo-2-(2-chlorophenyl)acetic acid (2) [00520] A 100-mL round bottom flask was charged with 2-amino-2-(2- chlorophenyl)acetic acid (2.22 g, 11.9 mmol) and 6 N HBr (40 mL). Sodium nitrite (1.57 g, 22.8 mmol) was added portionwise at 0 0C over 1 h 20 min. After 6 h, the reaction mixture was poured onto water and extracted with 3:1 Et2OZEtOAc (3x 80 mL). The combined organic layers were washed with water, sodium thiosulfate (2 x 50 mL), water, brine, dried over MgSO4. After purification by flash chromatography (75:25:0.5:0.35 hexanes/ethyl acetate/acetic acid/methanol), the title compound was obtained as a solid (2.161 g), 73% yield over two steps.
1H NMR (400 MHz, CDCl3) δ 11.58 (br s, IH), 7.77 (m, IH), 7.40 (m, IH), 7.31 (m, 2H), 5.94 (s, IH); 13C NMR (100 MHz, CDCl3) δ 174.0, 133.5, 133.2, 131.3, 130.0, 130.0, 127.9, 42.7.
5-Bromo-3-(2-bromo-2-(2-chlorophenyl)acetamido)benzofuran-2-carboxamide (5) [00521] The title compound was synthesized in a manner similar to Example 17, wherein 2-bromo-3 -methyl butanoic acid was substituted with 2-bromo-2-(2- chlorophenyl)acetic acid. After purification by flash chromatography (95:4.5:0.5 dichloromethane/methanol/28% (w/w) ammonium hydroxide), the title compound was obtained as a solid (357 mg), 87% yield.
1H NMR (400 MHz, J6-DMSO) δ 11.08 (br s, IH), 8.29 (br d, IH), 8.15 (m, IH), 8.01 (br d, IH), 7.83 and 7.72 (m, IH), 7.65-7.69 (m, IH), 7.62 (m, IH), 7.51-7.59 (m, IH), 7.45 (m, 2H), 6.45 (s, IH); MS (ESI+) for Ci7HnBr2ClN2O3: 487 (MH+). 5-Bromo-3-(2-(2-chlorophenyl)-2-(4-methylpiperazin-l-yl)acetamido)benzofuran-2- carboxamide (6) [00522] The title compound was synthesized in a manner similar to Example 17, wherein 5 -bromo-3 -(2-bromo-3 -methylbutanamido)benzofuran-2-carboxamide was substituted with 5-bromo-3-(2-bromo-2-(2-chlorophenyl)acetamido)benzofuran-2- carboxamide and pyrrolidine with JV-methylpiperazine and diisopropylethylamine (1.12 equiv); chloroform (20 mL/mmol) and acetonitrile (20 mL/mmol) were also added. After purification by flash chromatography (95:4.5:0.5 to 90:9:1 dichloromethane/methanol/28% (w/w) ammonium hydroxide), the title compound was obtained as a solid (192 mg), quantitative yield.
1H NMR (400 MHz, CDCl3) δ 11.44 (s, IH), 8.71 (d, J= 2.5 Hz, IH), 7.47 (m, 3H), 7.28 (m, 3H), 6.59 (br s, IH), 6.36 (br s, IH), 5.00 (s, IH), 2.74 (m, 8H), 2.41 (s, 3H); 13C NMR (100 MHz, CDCl3) δ 169.7, 162.6, 152.3, 135.7, 133.1, 132.4, 131.6, 130.8, 130.4, 129.9, 129.8, 127.5, 127.3, 123.7, 116.4, 113.3, 70.2, 54.1, 50.7, 45.6; MS (ESI+) for C22H22BrClN4O3: 506 (MH+).
(Compound 156)
8-Bromo-2-[(2-chlorophenyl)(4-methylpiperazin-l-yl)methyll[llbenzofuro[3,2- </|pyrimidin-4(3/-/)-one [00523] The title compound was synthesized in a manner similar to Example 17, wherein 5 -bromo-3 -(3 -methyl-2-(pyrrolidin- 1 -yl)butanamido)benzofuran-2- carboxamide was substituted 5 -bromo-3 -(2-(2-chlorophenyl)-2-(4-methylpiperazin-l- yl)acetamido)benzofuran-2-carboxamide. After purification by flash chromatography (91 :8:1 dichloromethane/methanol/28% (w/w) ammonium hydroxide), the title compound was obtained as a solid (71 mg), 40% yield.
1H NMR (400 MHz, J6-DMSO) δ 8.10 (m, IH), 7.97 (d, J= 8 Hz, IH), 7.81 (m, 2H), 7.44 (m, 2H), 7.33 (m, IH), 5.07 (s, IH), 2.31-2.48 (m, 8H), 2.17 (s, 3H); MS (ESI+) for C22H20BrClN4O2: 489 (MH+). (Compound 68)
2-(4-amino-2-methylphenyl)-8-bromo[llbenzofuro[3,2-dlpyrimidin-4(3H)-one [00524] 2-(4-amino-2-methylphenyl)-8-bromo[l]benzofuro[3,2-d]pyrimidin- 4(3H)-one was synthesized in a manner similar to Scheme 11, wherein 2-chloro-4- nitrobenzaldehyde was substituted with 2-methyl-4-nitrobenzaldehyde. NMR (400 MHz, de-DMSO): 8.18 (s, IH), 7.80 (s, 2H), 7.24 (d, IH), 6.47 (m, 2H), 5.49 (s, 2H), 2.49 (s, 3H). MS (EI) for Ci7Hi2BrN3O2: 371 (MH+).
(Compound 72)
8-bromo-2-{2-methyl-4-r(piperidin-4-ylmethyl)aminolphenvUπibenzofuror3,2- dlpyrimidin-4(3H)-one
[00525] 8-bromo-2-{2-methyl-4-[(piperidin-4- ylmethyl)amino]phenyl}[l]benzofuro[3,2-d]pyrimidin-4(3H)-one was synthesized in a manner similar to Scheme 11, wherein 2-(4-amino-2-chlorophenyl)-8- bromo[l]benzofuro[3,2-d]pyrimidin-4(3H)-one was substituted with 2-(4-amino-2- methylphenyl)-8-bromo[l]benzofuro[3,2-d]pyrimidin-4(3H)-one. NMR (400 MHz, de-DMSO): 8.13 (s, IH), 7.77 (s, 2H), 7.33 (d, IH), 6.47 (m, 2H), 6.04 (m, IH), 3.06 (d, 2H), 2.96 (t, 2H), 2.55 (m, 2H), 2.40 (s, 3H), 1.73 (m, 3H), 1.17 (m, 2H). MS (EI) for C23H23BrN4O2: 468 (MH+).
(Compound 61) 8-bromo-2-(2-methyl-4-r(tetrahvdrofuran-3- ylmethyl)aminolphenyl| [llbenzofuro[3,2-dlpyrimidin-4(3H)-one was synthesized [00526] 8-bromo-2- {2-methyl-4-[(tetrahydrofuran-3- ylmethyl)amino]phenyl}[l]benzofuro[3,2-d]pyrimidin-4(3H)-one was synthesized in a manner similar to Scheme 11, wherein 2-(4-amino-2-chlorophenyl)-8- bromo[l]benzofuro[3,2-d]pyrimidin-4(3H)-one was substituted with 2-(4-amino-2- methylphenyl)-8-bromo[l]benzofuro[3,2-d]pyrimidin-4(3H)-one, and tert-butyi 4- formylpiperidine-1-carboxylate was substituted with tetrahydrofuran-3-carbaldehyde. NMR (400 MHz, d6-DMSO): 8.16 (s, IH), 7.79 (s, 2H), 7.34 (d, IH), 6.50 (s, 2H), 6.14 (m, IH), 3.75 (m, 2H), 3.64 (dd, IH), 3.47 (dd, IH), 3.05 (t, 2H), 2.39 (s, 3H), 1.99 (m, IH), 1.90 (br s, IH), 1.59 (m, IH). MS (EI) for C22H20BrN3O3: 455 (MH+). EXAMPLE 18:
Figure imgf000247_0001
wherein R3a is as described in the disclosure above. 8-bromo-2-thioxo-2,3-dihydrobenzofuro[3,2-dlpyrimidin-4(lH)-one [00527] To a solution of 3-amino-5-bromobenzofuran-2-carboxamide (0.500 g, 1.97 mmol) was added DBU (0.6 g, 3.94 mmol) and CS2 (4.25 g, 56.0 mmol). The mixture was heated to 60 0C for 12 hours. The reaction was cooled down to room temperature and acidified with IN HCl (50 mL). The precipitate was filtered off and washed with a large amount of water to afford 695 mg of the title compound (quantitative). 1H NMR (400 MHz, d6-DMSO): 13.54 (s, IH), 12.87 (s, IH), 8.34 (m, IH), 7.81 (m, 2H). MS (EI) for Ci0H5BrN2O2S: 295 (M-H). 8-bromo-2-(methylthio)benzofuro[3,2-d]pyrimidin-4(3H)-one
[00528] To a solution of 8-bromo-2-thioxo-2,3-dihydrobenzofuro[3,2-d]pyrimidin- 4(lH)-one (0.53 g, 1.80 mmol) was added aqueous IN NaOH (1.80 mL) and iodomethane (0.25 g, 1.80 mmol). The reaction mixture was heated to 40 0C for 90 minutes and then concentrated in vacuo. After addition of 100 mL of water, the precipitate was filtered off and dried to afford the title compound (440 mg, 79%). 1H NMR (400 MHz, d6-DMSO): 8.17 (m, IH), 7.81 (m, 2H), 2.64 (s, 3H).
(Compound 147) 8-bromo-2-morpholin-4-virilbenzofuror3,2-dlpyrimidin-4(3H)-one
[00529] A solution of 8-bromo-2-(methylthio)benzofuro[3,2-d]pyrimidin-4(3H)- one (51 mg, 0.165 mmol) in 1 mL morpholine was heated to 160 0C for 64 hours. The reaction mixture was cooled down to room temperature and partitioned between water and ethyl acetate. After extraction with ethyl acetate (2 x 50 mL), the combined organic phases were washed with brine, dried over Na2SO4 and concentrated in vacuo to afford the title compound (14.5 mg, 25%). 1U NMR (400 MHz, d6-DMSO): 8.05 (m, IH), 7.73 (m, 2H), 3.69 (m, 4H), 3.63 (m, 4H). MS (EI) for Ci4Hi2BrN3O3: 350 (MH+). EXAMPLE 19:
Figure imgf000248_0001
wherein R3a is as described in the disclosure above and R31 is as described in the compounds within this example.
(Compound 122) 8-bromo-2-(piperidin-4-ylmethyl)rilbenzofuror3,2-dlpyrimidin-4(3H)-one
[00530] To a suspension of 3-amino-5-bromobenzofuran-2-carboxamide (100 mg, 0.39 mmol) in DMSO (3 mL) was added tert-butyl 4-(2-oxoethyl)piperidine-l- carboxylate (177 mg, 0.78 mmol) and NaHSCh (121 mg, 1.17 mmol). The reaction mixture was heated to 160 0C for 2 hours, cooled to room temperature and partitioned between water and ethyl acetate. The reaction was extracted with ethyl acetate (2 x 150 mL) and the combined organic phases were washed with brine, dried over Na2SO4 and concentrated in vacuo. To a solution of the crude product in MeOH (2 mL) was added 4 N HCl/dioxane (2 mL) and stirred for 12 hours at room temperature. The reaction mixture was concentrated in vacuo and purified by preparative HPLC to afford the title compound (14.3 mg, 10%). 1H NMR (400 MHz, d6-DMSO): 8.14 (m, IH), 7.75 (m, 2H), 3.00 (m, 2H), 2.59 (m, 4H, overlapped), 2.02 (m, IH), 1.62 (m, 2H), 1.23 (m, 2H). MS (EI) for Ci6Hi6BrN3O2: 362.2 (MH+).
(Compound 168)
2- { r 1 -(2-aminoethvDpiperidin-4-yl"|methvU -8-bromo[ 1 "IbenzofuroB ,2-dlpyrimidin- 4(3H)-one
[00531] To a solution of 8-bromo-2-(piperidin-4-ylmethyl)[l]benzofuro[3,2- d]pyrimidin-4(3H)-one (Compound 122) (25 mg, 0.066 mmol) in dichloroethane (3 mL) was added tert-butyl 2-oxoethylcarbamate (25 mg, 0.132 mmol), NaBH(OAc)3 and acetic acid (300 μL) and stirred at room temperature for 12 hours. The reaction mixture was quenched with saturated aqueous NaHCO3 (50 mL) and extracted with dichloromethane (2 x 150 mL), The combined organic phases were washed with brine, dried over Na2SO4 and concentrated in vacuo. To a solution of the crude product in MeOH (2 mL) was added 4 N HCl/dioxane (2 mL) and stirred for 12 hours at room temperature. The reaction mixture was concentrated in vacuo and purified by preparative HPLC to afford the title compound (10.3 mg, 39%). 1H NMR (400 MHz, de-DMSO): 8.15 (m, IH), 7.77 (m, 2H), 2.81 (m, 3H), 2.66 (m, 3H), 2.57 (m, 2H), 2.31 (m, 2H), 1.84 (m, IH), 1.59 (m, 2H), 1.29 (m, 2H). MS (EI) for Ci8H2IBrN4O2: 405.2 (MH+). (Compound 194)
8-bromo-2-(piperidin-3-ylmethyl)[llbenzofuro[3,2-dlpyrimidin-4(3H)-one [00532] 8-bromo-2-(piperidin-3-ylmethyl)[l]benzofuro[3,2-d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 19, wherein tert-butyl 4-(2- oxoethyl)piperidine-l-carboxylate was substituted with tert-butyl 3-(2- oxoethyl)piperidine-l-carboxylate. The reaction mixture was concentrated in vacuo and purified by preparative HPLC to afford the title compound (64 mg, 22%). 1H NMR (400 MHz, d6-DMSO): 8.13 (m, IH), 7.77 (m, 2H), 2.99 (m, 2H), 2.56 (m, 2H), 2.38 (m, 2H), 2.07 (m, IH), 1.73 (m, IH), 1.61 (m, IH), 1.43 (m, IH), 1.15 (m, IH). MS (EI) for Ci6Hi6BrN3O2: 361.9 (MH+). (Compound 226)
8-bromo-2-[(l-methylpiperidin-3-yl)methyll[llbenzofuro[3,2-dlpyrimidin-4(3H)-one
[00533] To a solution of 8-bromo-2-(piperidin-3-ylmethyl)benzofuro[3,2- d]pyrimidin-4(3H)-one (Compound 194) (150 mg, 0.42 mmol) in 5 mL of water was added formaldehyde (37% wt in water) and formic acid (2 mL). The reaction mixture was heated to reflux for 5 hours, cooled down, basified to pH 9 with aqueous IN NaOH and extracted with chloroform (3 x 50 mL). The combined organic phases were washed with brine, dried over Na2SO4 and concentrated in vacuo. The crude material was purified by preparative HPLC to afford the title compound (42.5 mg, 27%). 1H NMR (400 MHz, d6-DMSO): 8.19 (m, IH), 7.81 (m, 2H), 2.67 (m, 2H), 2.60 (m, 2H), 2.11 (s, 3H), 2.09 (m, IH, overlappped), 1.84 (m, IH), 1.63 (m, 3H), 1.43 (m, IH), 0.94 (m, IH). MS (EI) for Ci7Hi8BrN3O2: 376 (MH+).
(Compound 244)
8-bromo-2-|Y 1 -methylpiperidin-4-yl)methyll [ 1 ]benzofuro[3 ,2-dlpyrimidin-4(3H)-one
[00534] To a solution of 8-bromo-2-(piperidin-4-ylmethyl)benzofuro[3,2- d]pyrimidin-4(3H)-one (Compound 122) (100 mg, 0.27 mmol) in 5 mL of water was added formaldehyde (37% wt in water) and formic acid (2 mL). The reaction mixture was heated to reflux for 5 hours, cooled down, basified to pH 9 with aqueous IN NaOH and extracted with chloroform (3 x 50 mL). The combined organic phases were washed with brine, dried over Na2SO4 and concentrated in vacuo. The crude material was purified by preparative HPLC to afford the title compound (18.3 mg, 17.6%). 1H NMR (400 MHz, d6-DMSO): 8.19 (m, IH), 7.80 (m, 2H), 2.72 (m, 2H), 2.60 (m, 2H), 2.12 (s, 3H), 1.80 (m, 3H), 1.58 (m, 2H), 1.25 (m, 2H). MS (EI) for Ci7Hi8BrN3O2: 376 (MH+).
(Compound 305)
8-bromo-2-[(3S)-piperidin-3-ylmethyll[llbenzofuro[3,2-dlpyrimidin-4(3H)-one [00535] 8-bromo-2-[(3S)-piperidin-3-ylmethyl][l]benzofuro[3,2-d]pyrimidin- 4(3H)-one was synthesized in a manner similar to Example 19, wherein tert-butyl A- (2-oxoethyl)piperidine-l-carboxylate was substituted with (S)-tert-butyl 3-(2- oxoethyl)piperidine-l-carboxylate. The reaction mixture was concentrated in vacuo and purified by preparative HPLC to afford the title compound (16 mg, 3.7%). 1H NMR (400 MHz, d6-DMSO): 8.14 (m, IH), 7.77 (m, 2H), 2.99 (m, 2H), 2.58 (m, 2H), 2.38 (m, 3H), 1.74 (m, IH), 1.63 (m, IH), 1.42 (m, IH), 1.17 (m, IH). MS (EI) for Ci6Hi6BrN3O2: 362 (MH+).
(Compound 315)
8-bromo-2-r(3R)-piperidin-3-ylmethyliπibenzofuror3,2-dlpyrimidin-4(3H)-one [00536] 8-bromo-2-[(3R)-piperidin-3-ylmethyl][l]benzofuro[3,2-d]pyrimidin- 4(3H)-one was synthesized in a manner similar to Example 19, wherein tert-butyl A- (2-oxoethyl)piperidine-l-carboxylate was substituted with (R)-tert-butyl 3-(2- oxoethyl)piperidine-l-carboxylate. The reaction mixture was concentrated in vacuo and purified by preparative HPLC to afford the title compound (7.6 mg, 2%). 1H NMR (400 MHz, d6-DMSO): 8.14 (m, IH), 7.84 (m, 2H), 3.23 (m, 2H), 2.64 (m, 2H), 2.33 (m, 3H), 1.78 (m, 2H), 1.59 (m, IH), 1.28 (m, IH). MS (EI) for Ci6Hi6BrN3O2: 362 (MH+). EXAMPLE 20
Figure imgf000250_0001
(Compound 195)
2-(aminomethyl)-8-bromoπibenzofuror3,2-dlpyrimidin-4(3H)-one [00537] To a solution of 8-bromo-2-(chloromethyl)benzofuro[3,2-d]pyrimidin- 4(3H)-one (510 mg, 1.63 mmol) in DMF (8 mL) was added potassium 1,3- dioxoisoindolin-2-ide (452 mg, 2.44 mmol). The reaction mixture was heated to 100 0C for an hour, cooled down to room temperature, diluted with water and extracted with choroform (3 x 100 mL). The combined organic phases were washed with brine, dried over Na2SO4, concentrated in vacuo and purified by SiO2 flash chromatography (50:50 to 30:70 hexanes/ethyl acetate) to afford 2-((8-bromo-4-oxo-3,4- dihydrobenzofuro[3,2-d]pyrimidin-2-yl)methyl)isoindoline-l,3-dione (160 mg, 25%). To a solution of 2-((8-bromo-4-oxo-3,4-dihydrobenzofuro[3,2-d]pyrimidin-2- yl)methyl)isoindoline-l,3-dione (160 mg, 0.378 mol) in THF (4 mL) was added hydrazine monohydrate (0.6 mL) and stirred at room temperature for 12 hours. The reaction mixture was concentrated in vacuo and purified by preparative HPLC to afford the title compound (9.2 mg, 8.3%). 1H NMR (400 MHz, d6-DMSO): 8.08 (m, IH), 7.72 (m, 2H), 3.80 (m, 2H). MS (EI) for CnH8BrN3O2: 292 (M-H). EXAMPLE 21
Figure imgf000251_0001
(Compound 46)
3-(8-bromo-4-oxo-3,4-dihvdrorilbenzofuror3,2-dlpyrimidin-2-yl)-N- cyclohexylbenzamide 3 methyl 3-(8-bromo-4-oxo-3,4-dihydrobenzofuro[3,2-dlpyrimidin-2-yl)benzoate 1 [00538] Methyl 3-(8-bromo-4-oxo-3,4-dihydrobenzofuro[3,2-d]pyrimidin-2- yl)benzoate was synthesized in the same manner as Example 8 wherein 2- chlorobenzaldehyde was replaced with methyl-3-formylbenzoate. Filtration of solids afforded 180 mg (45%) of title compound 1. 3-(8-bromo-4-oxo-3,4-dihydrobenzofuro[3,2-dlpyrimidin-2-yl)benzoic acid 2 [00539] To a solution of methyl 3-(8-bromo-4-oxo-3,4-dihydrobenzofuro[3,2- d]pyrimidin-2-yl)benzoate 1 (280 mg, 0.7 mmol) in 1 :1 tetrahydrofuran:water (8 mL) and methanol (0.7 mL) was added lithium hydroxide (75 mg, 1.8 mmol) and stirred at room temperature for 2 h. The reaction mixture was concentrated in vacuo. The concentrate was dissolved in water and acidified with concentrated HCl. The solids were filtered and dried to afford 58 mg (20%) of 3-(8-bromo-4-oxo-3,4- dihydrobenzofuro[3,2-d]pyrimidin-2-yl)benzoic acid 2. (Compound 92)
3-(8-bromo-4-oxo-3,4-dihydro[llbenzofuro[3,2-dlpyrimidin-2-yl)-N- cyclohexylbenzamide
[00540] To a solution of 3-(8-bromo-4-oxo-3,4-dihydrobenzofuro[3,2- d]pyrimidin-2-yl)benzoic acid 2 (58 mg, 0.15 mmol) in dichloromethane (5 mL) was added cyclohexylamine (30 mg, 0.3 mmol), l-(3-dimethylaminopropyl)-3- ethylcarbodiimide hydrochloride (32 mg, 0.17 mmol), HOBt (25 mg, 0.17 mmol), N- methylmorpholine (60 mg, 0.6 mmol) and stirred for 18 h at room temperature. The reaction was quenched with water and extracted with ethyl acetate (2 x 100 ml). The combined organic phases were washed with aqueous K2CO3, brine, dried over Na2SO4 and concentrated in vacuo to afford 29 mg of crude product. Purification by preparative HPLC (reverse-phase, acetonitrile/water with 0.1% formic acid), followed by concentration in vacuo and lyophilization afforded the title compound (2.0 mg, 2 %). NMR (400 MHz, d6-DMSO): 8.67 (s, IH), 8.39 (d, IH), 8.32 (d, IH), 8.21 (s, IH), 8.17 (s, IH), 7.86 (d, IH), 7.71 (s, IH), 7.53 (t, IH), 6.56-6.64 (s, br, IH), 3.75- 3.85 (s, br, 2H), 1.82-1.91 (m, 2H), 1.72-1.80 (m, IH), 1.59-1.64 (m, IH), 1.09-1.43 (m, 4H). MS (EI) for C23H20BrN3O2: 466 (MH+). EXAMPLE 22
Scheme
Figure imgf000252_0001
1 -bromo-3-(cvclopropylmethylamino)propan-2-one 2 [00541] To a solution of aminomethylcyclopropane (466 mg, 6.5 mmol) in acetonitrile (20 mL) and diisopropylethylamine (2.2 mL) was added bromoacetic acid (1.1 g, 7.9 mmol) and HATU (2.9 g, 7.6 mmol). The reaction mixture was stirred 15 h and the solids were filtered. The filtrate was concentrated in vacuo to afford 430 mg (35%) of crude l-bromo-3-(cyclopropylmethylamino)propan-2-one 2. 3-(3-(cyclopropylmethylamino)-2-oxopropoxy)benzaldehyde 3
[00542] To a solution of l-bromo-3-(cyclopropylmethylamino)propan-2-one 2 (290 mg, 1.5 mmol) in acetone (20 mL) at O0C was added 3-hydroxybenzaldehyde (185 mg, 1.5 mmol) and cesium carbonate (2.0 g, 6.1 mmol) and stirred for 3 h at room temperature. The reaction mixture was concentrated in vacuo, dissolved in ethyl acetate. The product was then purified by silica gel column using 1 : 1 hexanes: ethyl acetate as eluent, followed by concentration in vacuo and lyophilization to afford 240 mg (68%) of 3-(3-(cyclopropylmethylamino)-2- oxopropoxy)benzaldehyde 3. (Compound 98)
2-{[3-(8-bromo-4-oxo-3,4-dihydro[llbenzofuro[3,2-dlpyrimidin-2-yl)phenylloxy|-N-
(cvclopropylmethyl)acetamide [00543] 2-{[3-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2- yl)phenyl]oxy}-N-(cyclopropylmethyl)acetamide was synthesized in a similar manner to Example 8 wherein 2-chlorobenzaldehyde was replaced with 3-(3-
(cyclopropylmethylamino)-2-oxopropoxy)benzaldehyde 3. Purification by preparative HPLC (reverse-phase, acetonitrile/water with 0.1% formic acid), followed by concentration in vacuo and lyophilization afforded the title compound (3.9 mg).
NMR (400 MHz, d6-DMSO): 8.33 (s, IH), 8.23-8.28 (m, IH), 8.12 (s, IH), 7.93-7.98 (m, 2H), 7.68 (s, 2H), 7.30-7.39 (m, IH), 4.54 (s, 2H), 2.69-3.09 (m, 2H), 0.90-1.06
(m, IH), 0.32-0.46 (m, 2H), 0.13-0.24 (m, 2H). MS (EI) for C22HlSBrN3O4: 468
(MH+).
(Compound 99)
3-(8-bromo-4-oxo-3,4-dihydro[llbenzofuro[3,2-dlpyrimidin-2-yl)-N- (phenylmethyl)benzamide
[00544] 3-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-N- (phenylmethyl)benzamide was synthesized in a similar manner to Example 21 wherein cyclohexylamine was replaced with benzylamine. Purification by preparative HPLC (reverse-phase, acetonitrile/water with 0.1% formic acid), followed by concentration in vacuo and lyophilization afforded the title compound (2.8 mg). NMR (400 MHz, d6-DMSO): 13.02-13.24 (s, br, IH), 9.13-9.20 (m, IH), 8.68 (s, IH), 8.26-8.36 (m, 2H), 8.03-8.15 (m, 2H), 7.79-7.91 (m, 2H), 7.64-7.71 (m, IH), 7.21-7.40 (m, 4H), 4.49-4.56 (m, 2H). MS (EI) for C24Hi6BrN3O3: 474 (MH+).
(Compound 101)
3-(8-bromo-4-oxo-3,4-dihydro[llbenzofuro[3,2-dlpyrimidin-2-yl)-N-(2- methylpropyDbenzamide [00545] 3-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-N-(2- methylpropyl)benzamide was synthesized in a similar manner to Example 21 wherein cyclohexylamine was replaced with isobutylamine. Purification by preparative HPLC (reverse-phase, acetonitrile/water with 0.1% formic acid), followed by concentration in vacuo and lyophilization afforded the title compound (3.5 mg). NMR (400 MHz, d6-DMSO): 8.65 (s, IH), 8.55-8.60 (m, IH), 8.35 (d, IH), 8.24 (s, IH), 8.17 (s, IH), 7.95 (d, IH), 7.76-7.81 (m, 2H), 7.57-7.62 (m, IH), 3.09-3.18 (m, 2H), 1.83-1.94 (m, IH), 0.93 (d, 6H). MS (EI) for C2IHi8BrN3O3: 440 (MH+). EXAMPLE 23
Figure imgf000254_0001
X = N R2 Or OR wherein R2 and R are as described in the compounds within this example. (Compound 224) l-r(8-bromo-4-oxo-3,4-dihvdrorilbenzofuror3,2-dlpyrimidin-2-yl)methyll-N-r(2- chlorophenyl)methyllpyrrolidine-3-carboxamide
[00546] To a solution of l-[(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2- d]pyrimidin-2-yl)methyl]pyrrolidine-3-carboxylic acid (Compound 103) (40 mg, 0.1 mmol) in dimethylacetamide (2 mL) and diisopropylethylamine (130 mg, 1 mmol) was added 2-chlorobenzylamine (140 mg, 1 mmol) followed by HATU (380 mg, 1 mmol). The reaction mixture was stirred at room temperature for 2h. Formation of product was confirmed by LC/MS and the product was purified by preparatory HPLC (reverse-phase, acetonitrile/water with 0.1% NH4OACZACOH) to yield 9 mg (17%) of the title compound. NMR (400 MHz, d6-DMSO): 8.39 (t, IH), 8.21 (t, IH), 7.83 (m, 2H), 7.42 (d, IH), 7.28 (m, 2H), 4.32 (d, 2H), 3.68 (s, 2H), 2.94 (m, 2H), 2.75 (m, IH), 2.67 (m, IH), 2.61 (m, IH), 1.99 (m, 2H). MS (EI) for C23 H20 Br Cl N4 O3: 515 (MH+).
(Compound 264) l-[(8-bromo-4-oxo-3,4-dihydro[llbenzofuro[3,2-dlpyrimidin-2-yl)methyll-N-[(3- chlorophenyl)methyllpyrrolidine-3-carboxamide [00547] l-[(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2- yl)methyl]-N-[(3-chlorophenyl)methyl]pyrrolidine-3-carboxamide was synthesized in a manner similar to Example 22 wherein 2-chlorobenzylamine was substituted with 3-chlorobenzylamine. Purification by preparative HPLC (reverse-phase, acetonitrile/water with 0.1% formic acid), followed by concentration in vacuo and lyophilization afforded the title compound (15.4 mg). NMR (400 MHz, d6-DMSO): 13.35 (s, IH), 10.48-10.53 (s, br, IH), 8.78 (s, IH), 8.17-8.30 (m, IH), 7.85-7.93 (m, 2H), 7.31-7.40 (m, 2H), 7.25 (d, IH), 4.50-4.69 (m, 2H), 4.33 (d, 2H), 4.07 (s, 2H), 3.88 (s, 2H), 2.89 (s, IH), 1.98-2.35 (m, 2H). MS (EI) for C23H20BrClN4O3: 515 (MH+).
(Compound 266) l-[(8-bromo-4-oxo-3,4-dihydro[llbenzofuro[3,2-dlpyrimidin-2-yl)methyll-N- (phenylmethyl)pyrrolidine-3-carboxamide
[00548] l-[(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2- yl)methyl]-N-(phenylmethyl)pyrrolidine-3-carboxamide was synthesized in a manner similar to Example 22 wherein 2-chlorobenzylamine was substituted with benzylamine. Purification by preparative HPLC (reverse-phase, acetonitrile/water with 0.1% formic acid), followed by concentration in vacuo and lyophilization afforded the title compound (64 mg). NMR (400 MHz, d6-DMSO): 8.37 (t, IH), 8.21 (s, IH), 7.83 (m, 2H), 7.29 (m, 2H), 7.21 (m, 2H), 4.27 (d, 2H), 3.67 (s, 2H), 2.91 (m, 2H), 2.75 (m, IH), 2.62 (m, 2H), 1.99 (m, 2H). MS (EI) for C23 Hi0 Br N4 O3: 482 (MH+).
(Compound 267)
8-bromo-2- { [3 -(morpholm-4-ylcarbonvOpyrrolidin- 1 -yllmethyl) \ 1 lbenzofuro [3,2- dlpyrimidin-4(3H)-one [00549] 8-bromo-2- { [3 -(morpholin-4-ylcarbonyl)pyrrolidin- 1 - yl]methyl}[l]benzofuro[3,2-d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 22 wherein 2-chlorobenzylamine was substituted with morpholine. Purification by preparative HPLC (reverse-phase, acetonitrile/water with 0.1% formic acid), followed by concentration in vacuo and lyophilization afforded the title compound (27 mg). NMR (400 MHz, d6-DMSO): 8.13 (s, IH), 7.83 (m, 2H), 3.69 (s, 2H), 3.53 (br s, 8H), 2.91 (m, 2H), 2.75 (m, 2H), 2.59 (m, IH), 1.99 (m, 2H). MS (EI) for C20 H2i Br N4 O4: 462 (MH+).
(Compound 271) l-r(8-bromo-4-oxo-3.4-dihvdrorilbenzofuror3.2-d1pyrimidin-2-vπmethyl1-N-r(4- chlorophenyl)methyllpyrrolidine-3-carboxamide
[00550] l-[(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2- yl)methyl]-N-[(4-chlorophenyl)methyl]pyrrolidine-3-carboxamide was synthesized in a manner similar to Example 22 wherein 2-chlorobenzylamine was substituted with 4-chlorobenzylamine. Purification by preparative HPLC (reverse-phase, acetonitrile/water with 0.1% formic acid), followed by concentration in vacuo and lyophilization afforded the title compound (3 mg). NMR (400 MHz, d6-DMSO): 8.42 (t, IH), 8.20 (t, IH), 7.82 (m, 2H), 7.34 (d, 2H), 7.22 (d, 2H), 4.25 (d, 2H), 3.66 (s, 2H), 2.90 (m, 2H), 2.75 (m, IH), 2.67 (m, 2H), 1.94 (m, 2H). MS (EI) for C23 H20 Br Cl N4 O3: 515 (MH+).
(Compound 272)
8-bromo-2-( {3-IY4-hvdroxypiperidin- 1 -vDcarbonyllpyrrolidin- 1 - vUmethyl)rHbenzofuror3,2-dlpyrimidin-4(3H)-one [00551] 8-bromo-2-( {3-[(4-hydroxypiperidin- 1 -yl)carbonyl]pyrrolidin- 1 - yl}methyl)[l]benzofuro[3,2-d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 22 wherein 2-chlorobenzylamine was substituted with 4- hydroxypiperidine. Purification by preparative HPLC (reverse-phase, acetonitrile/water with 0.1% formic acid), followed by concentration in vacuo and lyophilization afforded the title compound (25 mg). NMR (400 MHz, d6-DMSO): 8.21 (m, IH), 8.14 (m, IH), 7.83 (m, 2H), 3.91 (s, 2H), 3.69 (br s, 2H), 3.14 (m, 2H), 2.97 (m, 3H), 2.75 (m, 2H), 2.60 (m, IH), 1.94 (m, 2H), 1.66 (m, 2H), 1.24 (m, 2H). MS (EI) for C2i H23 Br N4 O4: 476 (MH+). (Compound 325) l-[(8-bromo-4-oxo-3,4-dihydro[llbenzofuro[3,2-dlpyrimidin-2-yl)methyll-N- (pyridin-4-ylmethyl)pyrrolidine-3-carboxamide
[00552] l-[(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2- yl)methyl]-N-(pyridin-4-ylmethyl)pyrrolidine-3-carboxamide was synthesized in a manner similar to Example 22 wherein 2-chlorobenzylamine was substituted with pyridin-4-ylmethanamine. Purification by preparative HPLC (reverse-phase, acetonitrile/water with 0.1% formic acid), followed by concentration in vacuo and lyophilization afforded the title compound (3 mg). NMR (400 MHz, d6-DMSO): 8.58 (t, IH), 8.45 (d, 2H), 8.16 (m, IH), 7.78 (d, 2H), 7.20 (d, 2H), 4.28 (d, 2H), 3.64 (s, 2H), 2.91 (m, 2H), 2.71 (m, 3H), 1.98 (m, 2H). MS (EI) for C22 H20 Br N5 O3: 483 (MH+).
(Compound 411)
'l-r(8-bromo-4-oxo-3,4-dihvdrorilbenzofuror3,2-dlpyrimidin-2-yl)methyll-N-r(2- chlorophenyDmethyl] -3 -hydroxypyrrolidine-3 -carboxamide
[00553] 'l-[(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2- yl)methyl] -N-[(2-chlorophenyl)methyl] -3 -hydroxypyrrolidine-3 -carboxamide was synthesized in a manner similar to Example 22 wherein l-[(8-bromo-4-oxo-3,4- dihydro [ 1 ]benzofuro [3 ,2-d]pyrimidin-2-yl)methyl]pyrrolidine-3 -carboxylic acid (Compound 103) was substituted with l-[(8-bromo-4-oxo-3,4- dihydro [ 1 ]benzofuro [3 ,2-d]pyrimidin-2-yl)methyl]-3 -hydroxypyrrolidine-3 - carboxylic acid (Compound 414). Purification by preparative HPLC (reverse-phase, acetonitrile/water with 0.1% formic acid), followed by concentration in vacuo and lyophilization afforded the title compound (12 mg). NMR (400 MHz, d6-DMSO): 8.46 (t, IH), 8.21 (d, 2H), 7.84 (m, 2H), 7.43 (dd, IH), 7.27 (m, 3H), 4.35 (d, 2H), 3.75 (q, 2H), 2.99 (q, IH), 2.93 (d, IH), 2.84 (d, IH), 2.59 (q, IH), 2.30 (m, IH), 1.87 (m, IH). MS (EI) for C23 H20 BrCl N4 O4: 532 (MH+). EXAMPLE 23B
(Compound 269) Ethyl l-[(8-bromo-4-oxo-3,4-dihydro[llbenzofuro[3,2-dlpyrimidin-2- yl)methvHpyrrolidine-3-carboxylate [00554] To a solution of l-[(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2- d]pyrimidin-2-yl)methyl]pyrrolidine-3-carboxylic acid (Compound 103) (200 mg, 0.5 mmol) in ethanol (3 niL) was added 2 drops of cone, sulfuric acid and the reaction mixture was stirred at 85 0C for 3h. Formation of product was confirmed by LC/MS and the product was purified by preparatory HPLC (reverse-phase, acetonitrile/water with 0.1% NH4OAc/AcOH) to yield the title compound. NMR (400 MHz, d6- DMSO): 8.20 (s, IH), 7.81 (s, 2H), 4.05 (q, 2H), 3.37 (s, 2H), 3.04 (m, IH), 2.90 (t, IH), 2.75 (m, IH), 2.67 (m, 2H), 1.99 (m, 2H), 1.17 (t, 3H). MS (EI) for Ci8 Hi8 Br N3 O4: 421 (MH+).
(Compound 270) l-[(8-bromo-4-oxo-3,4-dihydro[llbenzofuro[3,2-dlpyrimidin-2- yl)methyl"|pyrrolidine-3-carboxamide
[00555] To a solution of ethyl l-[(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2- d]pyrimidin-2-yl)methyl]pyrrolidine-3-carboxylate (100 mg, 0.23 mmol) in ethanol (1 mL) was added 5 mL of 4N NH3 in MeOH and the reaction mixture was stirred at 95 0C in a sealed vessel for 16h. Formation of product was confirmed by LC/MS and the product was purified by preparatory HPLC (reverse-phase, acetonitrile/water with 0.1% NH4OAc/AcOH) to yield 64 mg (70%) of the title compound. NMR (400 MHz, de-DMSO): 8.19 (s, IH), 7.80 (s, 2H), 7.35 (s, IH), 6.85 (s, IH), 3.63 (s, 2H), 2.82 (m, 2H), 2.67 (m, 3H), 1.89 (m, 2H). MS (EI) for Ci6 Hj5 Br N4 O3: 392 (MH+).
EXAMPLE 24
(Compound 314)
N-[3-(8-bromo-4-oxo-3,4-dihydro[llbenzofuro[3,2-dlpyrimidin-2-yl)-4- chlorophenvHpyridine-4-carboxamide
Figure imgf000258_0001
wherein R is as described within the compounds within this example. tert-Butyl 3 -(5 -bromo-2-carbamoylbenzofuran-3 -ylcarbamoyl)-4- chlorophenylcarbamate
[00556] tert-Butyl 3-(5-bromo-2-carbamoylbenzofuran-3-ylcarbamoyl)-4- chlorophenylcarbamate was synthesized in a manner similar to (Compound 198) wherein 5-(tert-butoxycarbonylamino)-2-chlorobenzoic acid (CNH Technologies Inc.) was substituted with Boc-3-azetidine carboxylic acid. The crude material from this reaction was submitted to the next step without any further purification. 1H NMR (400 MHz, d6-DMSO):10.73 (br s, IH), 9.76 (br s, IH), 8.30 (s, IH), 8.26 (s,lH), 8.00 (br s, IH), 7.88 (s, 2H), 7.70 (dd, IH), 7.63 (d, IH), 7.57 (dd, IH), 7.49 (d, IH), 1.48 (s, 9H). MS (EI) for C21 H19 Br Cl N3 O5: 509 (MH+).
2-(5-Amino-2-chlorophenyl)-8-bromoπibenzofuror3,2-dlpyrimidin-4(3H)-one [00557] tert-Butyl 3-(5-bromo-2-carbamoylbenzofuran-3-ylcarbamoyl)-4- chlorophenylcarbamate (15.92 mmoles, 8.1 g), was dissolved in ethanol ( 200 ml) and treated with aqueous IM potassium hydroxide solution (60 mmoles, 60ml). The reaction mixture was then refluxed for 18 hours, allowed to cool to ca. 60 0C, and acidified to pH =1, by the drop wise addition of concentrated hydrochloric acid, which resulted in the formation of a white precipitate. The resulting suspension was then diluted with additional ethanol (100ml) and heated to 100 0C for a further 8 hours. The reaction mixture was then allowed to cool and the resulting solid was filtered off, washed with cold ethanol and dried under reduced pressure to give 4.97 of 2-(5-amino-2-chlorophenyl)-8-bromo[l]benzofuro[3,2-d]pyrimidin-4(3H)-one as its hydrochloride salt. 1H NMR (400 MHz, d6-DMSO): 13.44 (br s, IH), 8.25 (7.89 (dd, IH), 7.85 (dd, IH), 7.56 (d, IH), 7.35 (s, IH), 7.28 (dd, IH), 6.82 (br s, 3H).MS (EI) for C16 H9 Br Cl N3 O2: 391 : (MH+).
N-[3-(8-bromo-4-oxo-3,4-dihydro[llbenzofuro[3,2-dlpyrimidin-2-yl)-4- chlorophenyllpyridine-4-carboxamide
[00558] N-[3-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-4- chlorophenyl]pyridine-4-carboxamide was synthesized in a manner similar to Example 11 and Example 11 + coupling, wherein 3-dimethylaminopropionic acid was substituted with isonicotinic acid and 2-(4-amino-2-chlorophenyl)-8- bromo[l]benzofuro[3,2-d]pyrimidin-4(3H)-one was substituted with 2-(5-amino-2- chlorophenyl)-8-bromo[l]benzofuro[3,2-d]pyrimidin-4(3H)-one. Purification by preparative HPLC (reverse-phase, acetonitrile/water with 0.1% formic acid), followed by concentration in vacuo and lyophilization afforded the title compound (50.0 mg). NMR (400 MHz, d6-DMSO): 13.41 (s, IH), 11.02 (s, IH), 8.87 (d, 2H), 8.26 (s, IH), 8.12 (d, IH), 8.00 (d, 2H), 7.83-7.94 (m, 3H), 7.72 (d, IH). MS (EI) for C22Hi2BrClN4O3: 495 (MH+). (Compound 326)
N-[3-(8-bromo-4-oxo-3,4-dihydro[llbenzofuro[3,2-dlpyrimidin-2-yl)-4- chlorophenyll-2-chloropyridine-4-carboxamide
[00559] N-[3-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-4- chlorophenyl]-2-chloropyridine-4-carboxamide was synthesized in a manner similar to Example 24 (Compound 314) wherein isonicotinic acid was substituted with 2- chloroisonicotinic acid. Purification by preparative HPLC (reverse-phase, acetonitrile/water with 0.1% formic acid), followed by concentration in vacuo and lyophilization afforded the title compound (19.5 mg). NMR (400 MHz, d6-DMSO): 10.84 (s, IH), 8.62-8.66 (m, IH), 8.18 (d, IH), 8.02-8.07 (m, 2H), 7.92-7.96 (m, IH), 7.88-7.91 (m, IH), 7.77-7.85 (m, 2H), 7.61 (d, IH), 6.61 (s, IH). MS (EI) for C22HnBrCl2N4O3: 530 (MH+).
(Compound 329)
N-r3-(8-bromo-4-oxo-3,4-dihvdrorilbenzofuror3,2-dlpyrimidin-2-yl)-4- chlorophenvHpyrimidine-5-carboxamide
[00560] N-[3-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-4- chlorophenyl]pyrimidine-5-carboxamide was synthesized in a manner similar to Example 24 wherein isonicotinic acid was substituted with 5-pyrimidine carboxylic acid. Purification by preparative HPLC (reverse-phase, acetonitrile/water with 0.1% formic acid), followed by concentration in vacuo and lyophilization afforded the title compound (13.8 mg). NMR (400 MHz, d6-DMSO): 10.92 (s, IH), 9.30 (d, 2H), 8.22-8.24 (m, IH), 8.09-8.12 (m, IH), 7.94-7.98 (m, IH), 7.81-7.90 (m, 2H), 7.64- 7.68 (m, IH), 6.61 (s, IH). MS (EI) for C21HnBrClN5O3: 496 (MH+).
(Compound 342) N-r3-(8-bromo-4-oxo-3,4-dihvdrorilbenzofuror3,2-dlpyrimidin-2-yl)-4- chlorophenyl]furan-3-carboxamide [00561] N-[3-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-4- chlorophenyl]furan-3-carboxamide was synthesized in a manner similar Example 24 wherein wherein isonicotinic acid was substituted with 3-furoic acid. Purification by preparative HPLC (reverse-phase, acetonitrile/water with 0.1% formic acid), followed by concentration in vacuo and lyophilization afforded the title compound (12.0 mg).
NMR (400 MHz, d6-DMSO): 10.22 (s, IH), 8.43-8.43 (m, IH), 8.20 (d, IH), 8.00 (d, IH), 7.90-7.95 (m, IH), 7.78-7.85 (m, 3H), 7.60 (d, IH), 6.95 (s, IH). MS (EI) for C2IHnBrClN3O4: 484 (MH+).
(Compound 356)
4-(acetylamino)-N-r3-(8-bromo-4-oxo-3,4-dihvdrorilbenzofuror3,2-dlpyrimidin-2- yl)-4-chlorophenyllbenzamide
[00562] 4-(acetylamino)-N-[3-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2- d]pyrimidin-2-yl)-4-chlorophenyl]benzamide was synthesized in a manner similar to Example 24 wherein wherein isonicotinic acid was substituted with 4- acetamidobenzoic acid. Purification by preparative HPLC (reverse-phase, acetonitrile/water with 0.1% formic acid), followed by concentration in vacuo and lyophilization afforded the title compound (6.9 mg). NMR (400 MHz, d6-DMSO):
10.42 (s, IH), 10.27 (s, IH), 8.22 (d, IH), 8.12 (d, IH), 7.92-7.98 (m, 3H), 7.81-7.88 (m, 2H), 7.70-7.75 (m, 2H), 7.59 (d, IH), 6.59 (s, IH), 2.09 (s, 3H). MS (EI) for C25Hi6BrClN4O4: 551 (MH+). (Compound 357)
N-[3-(8-bromo-4-oxo-3,4-dihydro[llbenzofuro[3,2-dlpyrimidin-2-yl)-4- chlorophenyl] -6-(methyloxy)pyridine-3 -carboxamide
[00563] N-[3-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-4- chlorophenyl]-6-(methyloxy)pyridine-3 -carboxamide was synthesized in a manner similar Example 24 wherein isonicotinic acid was substituted with 6- methoxynicotinic acid. Purification by preparative HPLC (reverse-phase, acetonitrile/water with 0.1% formic acid), followed by concentration in vacuo and lyophilization afforded the title compound (29.6 mg). NMR (400 MHz, d6-DMSO):
13.43 (s, IH), 10.58 (s, IH), 8.81 (d, IH), 8.23-8.27 (m, 2H), 8.11 (d, IH), 7.95-7.99 (m, IH), 7.82-7.91 (m, 2H), 7.63 (d, IH), 6.98 (d, IH). MS (EI) for C23Hi4BrClN4O4:
525 (MH+).
(Compound 327)
N-r3-(8-bromo-4-oxo-3,4-dihvdrorilbenzofuror3,2-dlpyrimidin-2-yl)-4- chlorophenyllpyridine-3-carboxamide [00564] N-[3-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-4- chlorophenyl]pyridine-3 -carboxamide was synthesized in a manner similar to Example 24 wherein isonicotinic acid was substituted with nicotinic acid.
Purification by preparative HPLC (reverse-phase, acetonitrile/water with 0.1% formic acid), followed by concentration in vacuo and lyophilization afforded the title compound (6.9 mg). NMR (400 MHz, d6-DMSO): 10.77 (s, IH), 9.13 (s, IH), 8.78 (d, IH), 8.31 (dt, IH), 8.24 (d, IH), 8.14 (d, IH), 7.97 (dd, IH), 7.88 (d, IH), 7.84 (dd, IH), 7.64 (d, IH), 7.58 (dd, IH). MS (EI) for C22Hi2BrClN4O3: 496 (MH+). (Compound 360)
N-[4-(8-bromo-4-oxo-3,4-dihydro[llbenzofuro[3,2-dlpyrimidin-2-yl)phenyllpyridine- 4-carboxamide
[00565] N-[4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2- yl)phenyl]pyridine-4-carboxamide was synthesized in a manner similar to Example 24 wherein isonicotinic acid was substituted with nicotinic acid, and 5-(tert- butoxycarbonylamino)-2-chlorobenzoic acid was substituted with 5-(tert- butoxycarbonylamino)-benzoic acid. Purification by preparative HPLC (reverse- phase, acetonitrile/water with 0.1% formic acid), followed by concentration in vacuo and lyophilization afforded the title compound (6.2 mg). NMR (400 MHz, d6- DMSO): 13.13 (s, IH), 10.81 (s, IH), 8.81 (d, IH), 8.22-8.30 (m, 2H), 7.96-8.01 (m, 2H), 7.81-7.93 (m, 3H), 6.57 (s, IH). MS (EI) for C22Hi3BrN4O3: 461 (MH+).
(Compound 361)
N-r3-(8-bromo-4-oxo-3,4-dihvdrorilbenzofuror3,2-dlpyrimidin-2-yl)-4- chlorophenylltetrahvdrofuran-3-carboxamide [00566] N-[3-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-4- chlorophenyl]tetrahydrofuran-3-carboxamide was synthesized in a manner similar to Example 24 wherein isonicotinic acid was substituted with tetrahydro-3-furoic acid. Purification by preparative HPLC (reverse-phase, acetonitrile/water with 0.1% formic acid), followed by concentration in vacuo and lyophilization afforded the title compound (34.3 mg). NMR (400 MHz, d6-DMSO): 13.39 (s, IH), 10.39 (s, IH),
8.24 (d, IH), 7.98 (d, IH), 7.83-7.91 (m, 2H), 7.72-7.77 (m, IH), 7.57 (d, IH), 3.94 (t, IH), 3.67-3.81 (m, 3H), 2.01-2.16 (m, 2H). MS (EI) for C2IHi5BrClN3O4: 488 (MH+).
(Compound 392)
'N-r3-(8-bromo-4-oxo-3,4-dihvdrorilbenzofuror3,2-dlpyrimidin-2-yl)-4- chlorophenyllpiperidine-4-carboxamide
[00567] 'N-[3-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-4- chlorophenyl]piperidine-4-carboxamide was synthesized in a manner similar to
Example 24 wherein 3-amino-5-bromobenzofuran-2-carboxamide 3 was substituted with 2-(5-Amino-2-chlorophenyl)-8-bromo[l]benzofuro[3,2-d]pyrimidin-4(3H)-one. Purification by preparative HPLC (reverse-phase, acetonitrile/water with 0.1% formic acid), followed by concentration in vacuo and lyophilization afforded the title compound (34.3 mg). NMR (400 MHz, d6-DMSO): 10.3 (s, IH), 8.11 (d, IH), 7.75 (m, 2H), 7.70 (m, IH), 7.44 (d, IH), 3.25-3.36 (m, 3H), 2.83 (m, 2H), 1.88 (m, 2H), 1.75 (m, 2H). MS (EI) for C22Hi8BrClN4O3: 501 (MH+).
(Compound 337)
N-[3-(8-bromo-4-oxo-3,4-dihydro[llbenzofuro[3,2-dlpyrimidin-2-yl)-4- chlorophenvH-2-(methyloxy)pyridine-4-carboxamide
[00568] N-[3-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-4- chlorophenyl]-2-(methyloxy)pyridine-4-carboxamide was synthesized in a manner similar to Example 24 wherein 2-methoxyisonicotinic acid replaced isonicotinic acid. IH NMR (400 MHz, d6-DMSO): 13.45 (s, br, IH), 10.78 (s, IH), 8.37 (d, IH), 8.25 (s, IH), 8.14 (d, IH), 7.98 (dd, IH), 7.88 (m, 2H), 7.66 (d, IH), 7.46 (dd, IH), 7.34 (s, IH), 3.91 (s, 3H). MS (EI) for C23 H14 Br Cl N4 04: 527 (MH+).
(Compound 338)
5-bromo-N-r3-(8-bromo-4-oxo-3,4-dihvdrorilbenzofuror3,2-dlpyrimidin-2-yl)-4- chlorophenyllpyridine-2-carboxamide
[00569] 5-bromo-N-[3-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin- 2-yl)-4-chlorophenyl]pyridine-2-carboxamide was synthesized in a manner similar to Example 24 wherein 5-bromopicolinic acid replaced isonicotinic acid. IH NMR (400 MHz, d6-DMSO): 13.47 (s, br, IH), 11.08 (s, IH), 8.90 (s, IH), 8.35 (m, 2H), 8.26 (s, IH), 8.09 (d, 2H), 7.88 (m, 2H), 7.64 (d, IH). MS (EI) for C22 Hl 1 Br2 Cl N4 03: 576 (MH+). (Compound 340)
N-r3-(8-bromo-4-oxo-3,4-dihvdrorilbenzofuror3,2-dlpyrimidin-2-yl)-4- chlorophenyl] -5 -chloropyridine-2-carboxamide
[00570] N-[3-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-4- chlorophenyl] -5 -chloropyridine-2-carboxamide was synthesized in a manner similar to Example 24 wherein 5-chloropicolinic acid replaced isonicotinic acid IH NMR (400 MHz, d6-DMSO): 13.48 (s, br, IH), 11.07 (s, IH), 8.81 (s, IH), 8.35 (s, IH), 8.26 (s, IH), 8.22 (d, IH), 8.17 (d, IH), 8.09 (d, IH), 7.88 (m, 2H), 7.64 (d, IH). MS (EI) for C22 Hl 1 Br C12 N4 03: 531 (MH+).
(Compound 371)
N-r3-(8-bromo-4-oxo-3,4-dihvdrorilbenzofuror3,2-dlpyrimidin-2-yl)-4- chlorophenyllbenzamide
[00571] N-[3-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-4- chlorophenyljbenzamide was synthesized in a manner similar to Example 24 wherein benzoyl chloride replaced isonicotinoyl chloride. IH NMR (400 MHz, d6- DMSO):13.47 (s, IH), 10.62 (s, IH), 8.25 (s, IH), 8.17 (s, IH), 7.98 (m, 3H), 7.87 (m, 2H), 7.63 (m, 2H), 7.56 (m, 2H). MS (EI) for C23 H13 Br Cl N3 03: 496 (MH+).
(Compound 372)
N-[3-(8-bromo-4-oxo-3,4-dihydro[llbenzofuro[3,2-dlpyrimidin-2-yl)-4- chlorophenyllisoxazole-5-carboxamide
[00572] N-[3-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-4- chlorophenyl]isoxazole-5-carboxamide was synthesized in a manner similar to Example 24 wherein isoxazole-5-carboxylic acid replaced isonicotinic acid. IH NMR (400 MHz, d6-DMSO): 11.07 (s, IH), 8.85 (d, IH), 8.21 (s, IH), 8.10 (d, IH), 7.95 (dd, IH), 7.84 (m, 2H), 7.64 (d, IH), 7.31 (d, IH). MS (EI) for C20 HlO Br Cl N4 04: 487 (MH+).
Figure imgf000264_0001
EXAMPLE 25
(Compound 128)
8-bromo-2-r2-chloro-4-(methylsulfonyl)phenylirilbenzofuror3,2-dlpyrimidin-4(3H)- one [00573] 2-chloro-4-(methylsulfonyl)benzoic acid (300 mg, 1.28 mmol) was dissolved in 5 ml of thionyl chloride and the reaction mixture was heated to 70 0C for 1 hour. The reaction mixture was concentrated down under reduced pressure, and the white solid was dissolved in 5 ml of pyridine, and 3-amino-5-bromobenzofuran-2- carboxamide 3 ( 315 mg, 1.24 mmol) was added at room temperature. The reaction was stirred at room temperature for 1 hr, followed by addition of 10 ml of MeOH. The resulting precipitate was filtered off and dissolved in 3 ml of EtOH. The reaction was stirred for 12 hours at 120 0C in a sealed pressure tube. The reaction was cooled and then neutralized with IM HCl until the pH 7. The resulting precipitate was filtered and washed with 5 ml OfH2O. The product was purified by preparatory HPLC (reverse-phase, acetonitrile/water with 0.1% NH40Ac/Ac0H) to yield 34.7 mg (12%) of the title compound. 1H NMR (400 MHz, d6-DMSO): 13.49 (s, IH), 8.25 (d, IH), 8.21 (d, IH), 8.07 (dd, IH), 8.00 (d, IH), 7.89 (m, 2H), 3.41 (s, 3H); MS (EI) for Ci7Hi0BrClN2O4S: 455 (MH+).
(Compound 219)
8-bromo-2- (3 -r(2-methylpropyl)oxylpyridin-4-yl| \ 1 lbenzofuro[3 ,2-dlpyrimidin- 4(3HVone [00574] 8-bromo-2-{3-[(2-methylpropyl)oxy]pyridin-4-yl} [l]benzofuro[3,2- d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 11, wherein 3-isobutoxyisonicotinic acid (Note A) replaced 2-chloro-4-(methylsulfonyl)benzoic acid. 1H-NMR (400MHz, d6-DMSO): 13.02 (s, IH), 8.58 (s, IH), 8.38 (d, IH), 8.23 (s, IH), 7.86 (m, 2H), 7.66 (d, IH), 4.00 (d, 2H), 1.99 (m, IH), 0.93 (d, 6H). MS (EI) for Ci9Hi6BrN3O3: 415 (MH+).
Note A : 3-isobutoxyisonicotinic acid
[00575] Sodium metal (7.3 g, 0.32 mol) was added in small portions to 2-methyl-l- propanol (145 mL, 0.63 mol) at 80 0C over 30 min period, and the reaction mixture was stirred for an additional 3 h at 80 0C. Subsequently, a solution of 3- chloroisonicotinic acid (1Og, 63 mmol) in 5 mL of DMSO was added to the reaction mixture at 80 0C. The resulting slurry was heated to 120 0C for an additional 16h, then cooled down to room temperature, concentrated down to half volume under the reduced pressure, and filterred. The filtrate was concentrated down to half volume under the reduced pressure, and filterred again. The combined solids were mixed with 10 mL of MeOH and 1 mL of water and acidified with concentrated HCl at 0 0C to pH = 7. The resulting precipitate was filterred out and dried under vacuum, resulting in 10 g ( 81%) of 3-isobutoxyisonicotinic acid. 1H-NMR (400MHz, d6- DMSO): 8.47 (s, IH), 8.23 (d, IH), 7.46 (d, IH), 3.91 (d, 2H), 2.04 (m, IH), 0.94 (d, 6H). MS (EI) for Ci0 Hi3NO3: 196 (MH+).
(Compound 148)
8-bromo-2-(4-chloropyridin-3-yl)rilbenzofuror3,2-dlpyrimidin-4(3H)-one [00576] 8-bromo-2-(4-chloropyridin-3-yl)[l]benzofuro[3,2-d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 25 wherein 4-chloronicotinic acid replaced 2-chloro-4(methylsulfonyl)benzoic acid. IH NMR (400 MHz, d6-DMSO): 13.55 (s, br, IH), 8.89 (s, IH), 8.70 (d, IH), 8.25 (s, IH), 7.75 (m, 3H). MS (EI) for C15 H7 Br Cl N3 02: 378 (MH+). (Compound 282)
8-bromo-2- ((3R)-5-oxo- 1 -[(1R)- 1 -phenylethyllpyrrolidin-3-vU \ 1 Ibenzofuror3.2- d"|pyrimidin-4(3H)-one
[00577] 8-bromo-2- {(3R)-5-oxo- 1 -[(1R)- 1 -phenylethyl]pyrrolidin-3- yl}[l]benzofuro[3,2-d]pyrimidin-4(3H)-one_was synthesized in a manner similar to Example 25 wherein (l'R, 3R)-l-(l'-phenylethyl)-5-oxo-3-pyrrolidine carboxylic acid replaced 2-chloro-4-(methylsulfonyl)benzoic acid.^H NMR (400 MHz, d6- DMSO): 8.18 (s, IH), 7.82 (s, 2H), 7.41-7.25 (m, 5H), 5.35-5.29 (m, IH), 3.80-3.60 (m, 2H), 3.42-3.35 (m, IH), 2.85-2.70 (m, 2H), 1.52 (d, 2H); MS (EI) for C22Hi8BrN3O3: 452 (MH+). (Compound 423)
2-(lH-benzimidazol-6-yl)-8-bromo[llbenzofuro[3,2-dlpyrimidin-4(3H)-one [00578] 2-(lH-benzimidazol-6-yl)-8-bromo[l]benzofuro[3,2-d]pyrimidin-4(3H)- one was synthesized in a manner similar to Example 25 wherein benzimidazole-5- carboxylic acid replaced 2-chloro-4(methylsulfonyl)benzoic acid. 1H NMR (400 MHz, d6-DMSO): 12.77 (s, IH), 12.68 (s, IH), 8.48 (s, IH), 8.32 (m, IH), 8.22 (m, IH), 8.07 (m, IH), 7.76 (m, 2H), 7.65 (m, IH). MS (EI) for C17 H9 Br N4 02: 382 (MH+). Example 26
Figure imgf000267_0001
(Compound 345)
N-[4-(8-bromo-4-oxo-3,4-dihydro[llbenzofuro[3,2-dlpyrimidin-2-yl)-3- chlorophenyli -5 -(hvdroxymethyl)pyridine-2-carboxamide
[00579] To a solution of methyl 6-({[4-(8-bromo-4-oxo-3,4- dihydro [ 1 ]benzofuro [3 ,2-d]pyrimidin-2-yl)-3 -chlorophenyl] amino } carbonyl)pyridine- 3-carboxylate (Compound 334) (20 mg, 0.036 mmol) in diethyl ether (5 mL) was added 0.5 mL Of LiAlH4 1 N solution in THF ( 0.25 mmol) and the reaction mixture was stirred at RT for 16 h. The resulting slurry was quenched with IN HCl (2 mL) and the product was purified by preparatory HPLC (reverse-phase, acetonitrile/water with 0.1% formic acid) to yeild 3mg (16%) of the title compound. 1H NMR (400 MHz, de-DMSO): 10.96 (s, IH), 8.71 (s, IH), 8.17 (m, 3H), 7.96 (m, 2H), 7.74 (m, 2H), 7.59 (d, IH), 6.60 (s, IH), 5.56 (t, IH), 4.69 (d, 2H); MS (EI) for C23Hi4BrClN4O4: 526 (MH+).
(Compound 347)
6-({r4-(8-bromo-4-oxo-3,4-dihvdrorilbenzofuror3,2-dlpyrimidin-2-yl)-3- chlorophenyl] amino I carbonyl)pyridine-3 -carboxylic acid
[00580] To a solution of methyl 6-({[4-(8-bromo-4-oxo-3,4- dihydro [ 1 ]benzofuro [3 ,2-d]pyrimidin-2-yl)-3 -chlorophenyl] amino } carbonyl)pyridine-
3-carboxylate (Compound 334) (180 mg, 0.32 mmol) in THF (2 mL), MeOH (0.5 mL), and H2O (1 mL) was added 0.1 g of LiOH and the reaction mixture was stirred at RT for 16 h. The resulting slurry was acidified with IN HCl (2 mL) to pH 6. The product was filtered out and submitted to the next step without further purification. The portion of the product was purified by preparatory HPLC (reverse-phase, acetonitrile/water with 0.1% formic acid) to yeild the title compound. 1H NMR (400 MHz, d6-DMSO): 11.24 (s, IH), 9.18 (s, IH), 8.49 (d, IH), 8.29 (m, 3H), 8.08 (dd, IH), 7.88 (m, 2H), 7.70 (d, IH); MS (EI) for C23Hi2BrClN4O5: 541 (MH+).
(Compound 394)
'N'5'-(2-aminoethvn-N'2'-r4-(8-bromo-4-oxo-3.4-dihvdrorilbenzofuror3.2- dlpyrimidin-2-yl)-3-chlorophenyllpyridine-2,5-dicarboxamide
[00581] To a solution of 6-({[4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2- d]pyrimidin-2-yl)-3-chlorophenyl]amino} carbonyl)pyridine-3-carboxylic acid
(Compound 347) (50mg, 0.09 mmol) in DMA (5 mL), and diisopropylethylamine
(0.5 mL) was added tert-butyl 2-aminoethylcarbamate (75 mg, 0.45 mmol) followed by HATU (170 mg, 0.45 mmol). The reaction mixture was stirred at RT for 16 h, then concentrated down under reduced pressure and re-dissolved in 5 mL of MeOH, and 5 mL of 4N HCl in Dioxane was added. The resulting mixture was stirred at RT for an additional 6 h and concentrated under reduced pressure. The product was purified by preparatory HPLC (reverse-phase, acetonitrile/water with 0.1% formic acid) to yeild the title compound. 1H NMR (400 MHz, d6-DMSO): 11.03 (s, IH),
9.14 (s, IH), 8.97 (s, IH), 8.45 (d, IH), 8.27 (d, IH), 8.19 (s, IH), 8.11 (s, IH), 7.95 (d, IH), 7.73 (m, 2H), 7.60 (d, IH), 6.60 (s, IH), 6.52 (s, IH), 3.17 (m, 2H), 2.87 (m,
2H); MS (EI) for C25Hi8BrClN6O4: 582 (MH+).
Example 26B (Compound 369) l-r4-(8-bromo-4-oxo-3,4-dihvdrorilbenzofuror3,2-dlpyrimidin-2-yl)-3- chlorophenyl] -3 -pyridin-4-ylurea
[00582] To a solution of 2-(4-amino-2-chlorophenyl)-8-bromo[l]benzofuro[3,2- d]pyrimidin-4(3H)-one (0.2g, 0.47 mmol) in pyridine (5 mL) was added 4- Nitrophenyl chloroformate (O.lg, 0.5 mmol). The reaction mixture was stirred at room temperature for Ih. The solvent was concentrated under reduced pressure. The residue was taken up in 1 mL of dimethylformamide, and 4-aminopyridine (0.23 g, 2.5 mmol) in 1 mL of dichloromethane was added. The resulting mixture was heated to 100 0C at 150 W for 10 minutes in a CEM-Discover microwave reactor. The solvent was concentrated under reduced pressure and the residue was purified by preparative HPLC (reverse-phase, acetonitrile/water with 0.1% formic acid) to yeild the title compound. IH NMR (400 MHz, d6-DMSO): 13.30 (s, IH), 10.58 (s, IH), 9.99 (s, IH), 8.59 (d, 2H), 8.24 (s, IH), 7.85 (m, 5H), 7.67 (d, IH), 7.53 (d, IH). MS (EI) for C22 Hi3 Br Cl N5 O3: 511 (MH+). (Compound 420)
8-bromo-2- { [(3 S)-3-hydroxypyrrolidin- 1 -yl"|methyl| -7-methyl[ 1 ]benzofuro|"3 ,2- d"|pyrimidin-4(3H)-one
[00583] 8-bromo-2- { [(3 S)-3-hydroxypyrrolidin- 1 -yl]methyl} -7- methyl[l]benzofuro[3,2-d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 1 wherein 4-methyl-5-bromo-2-hydroxybenzonitrile replaced 5-bromo-2- hydroxybenzonitrile 1. 8-Bromo-2- { [(3 S)-3-hydroxypyrrolidin- 1 -yljmethyl} -7- methyl[l]benzofuro[3,2-d]pyrimidin-4(3H)-one as mono HCl salt 1H NMR (400 MHz, de-DMSO): 13.27 (br s, IH), 10.71 (br s, IH), 8.21 (s, IH), 7.89 (s, IH), 5.51 (s, 2H), 4.52 (s, 3H), 3.74 (s, 2H), 3.37 (br s, 2H), 1.88-2.37 (m, 3H); MS (EI) for Ci6Hi6BrN3O3: 379 (MH+).
4-methyl-5-bromo-2-hydroxybenzonitrile
[00584] To a solution OfBCl3 (IM in dichloromethane, Aldrich, 12 niL, 12 mmol) was added a solution of 4-bromo-3 -methyl-phenol (1.87 g, 10 mmol) in 35 mL of dichloroethane, CH3SCN (0.88g, 12 mmol), and AlCl3 (1.33g, 10 mmol) at 0 0C. The mixture was heated to 80 0C for 4h under stirring. The reaction mixture was cooled down to room temperature and poured onto ice-4 N NaOH (33 mL), and heated to 80 0C for additional 30 min. Upon completion, the layers were separated, and the aqueous layer was acidified with 6N HCl and extracted with ether. The ether layer was washed with H2O and dried over MgSO4. Concentration under reduced pressure and re-crystallization from EtOAc/ Hexanes resulted in 0.95 g (47%) of the title compound. MS (EI) for C8H6BrNO: 202 (MH+) EXAMPLE 27
Figure imgf000270_0001
[00585] A solution of anti-7-hydroxy-2-azabicyclo[2.2.1]heptane 1 (0.75 g, 6.60 mmol, purchased from Tyger) and di-te/t-butyldicarbonate (2.17 g, 9.90 mmol) in dioxane/ 1 N NaOH (2: 1 , 75 mL) was stirred vigorously at room temperature for 24 hours. The solvent was evaporated and the residue was partitioned between DCM and water. The organic layer was dried and concentrated. The residue oil was purified by silica chromatography (hexanes/ EtOAc 3:2) to obtain 2 (racemic mixture). 1H NMR (400 MHz, CDCl3): 4.18 (s, IH), 3.95 (m, IH), 3.35 (m, IH), 3.00 (dd, IH), 2.30 (m, 1 H), 1.95 (m, 2H), 1.75 (m, IH), 1.65 (m, IH), 1.45 (s, 9H).
[00586] To a stirred solution of compound 2 (0.43 g, 2.0 mmol) in DCM (10 mL) at 0 0C was added 4-dimethylaminopyridine (2.0 mmol) followed by (R)-(-)-α- methyl- α-trifluoromethyl-phenylacetyl chloride (504 mg) over 2 min. The mixture was warmed to room temp and stirred overnight. Then the reaction mixture was quenched with water, extracted with EtOAc, washed with brine, dried and concentrated. The residue was purified by silica chromatography, eluted with hexanes/THF (9:1) which first gave 3a (280 mg), and further elution provided compound 3b (260 mg). Compound 3a: 1H NMR (400 MHz, CDCl3): 7.53 (m, 2H), 7.42 (m, 3H), 5.05 (d, IH), 4.20 (d, IH), 3.53 (s, 3H), 3.40 (m, IH), 3.08 (dd, IH), 2.60 (m, 2H), 1.80-1.60 (m, 3H), 1.45 (m, 9H).
Compound 3b: 1H NMR (400 MHz, CDCl3): 7.52 (m, 2H), 7.41 (m, 3H), 5.03 (d, IH), 4.20 (d, IH), 3.55 (s, 3H), 3.41 (m, IH), 3.07 (dd, IH), 2.60 (s, 2H), 1.84-1.60 (m, 3H), 1.43 (m, 9H). (Compound 413)
8-bromo-2-(r(7S)-7-hvdroxy-2-azabicvclor2.2.11hept-2-yllmethvUrilbenzofuror3.2- d1pyrimidin-4(3H)-one
[00587] To a solution of compound 3a (150 mg, 0.35 mmol) in EtOAc (10 niL) was added 4 N HCl in dioxane (3 mL) at 0 0C. The reaction mixture was allowed to warm up to room temperature and stirred 19 hours. The reaction was concentrated. The residue was dissolved in ethanol (15 mL); 8-bromo-2-
(chloromethyl)[l]benzofuro[3,2-d]pyrimidin-4(3H)-one 6 was added in one portion, followed by sodium bicarbonate (300 mg). The reaction mixture was heated to 80 0C for 4 hours. Then the reaction was cooled, 1 N NaOH (3 mL) was added. The reaction mixture was stirred at room temperature for 3 hours. Once the hydrolysis was complete, the reaction was neutralized with IN HCl. The reaction mixture was concentrated in vacuo and purified by preparative HPLC to give the final product. 1H NMR (400 MHz, d6-DMSO): 8.19 (s, IH), 7.82 (m, 2H), 4.45 (s, 1 H), 4.18 (s, 2H), 3.55 (m, 2H), 3.38 (m, IH), 2.96 (d, IH), 2.35 (s, IH), 2.05 (m, 2H), 1.60 (m, IH); MS (EI) for Ci7Hi6BrN3O3: 390 (MH+).
(Compound 412)
8-bromo-2-IY7-hvdroxy-2-azabicvclor2.2.1 lhept-2-vDmethyll \ 11benzofuror3.2- dlpyrimidin-4(3H)-one [00588] 8-bromo-2-[(7-hydroxy-2-azabicyclo[2.2.1]hept-2- yl)methyl][l]benzofuro[3,2-d]pyrimidin-4(3H)-one was synthesized in a manner similar to Compound 413, wherein 3a was substituted with 3b. Purification by preparative HPLC (reverse-phase, acetonitrile/water with 0.01% ammonium acetate), followed by concentration in vacuo and lyophilization afforded the title compound as a white solid. 1H NMR (400 MHz, CD3OD): 8.15 (s, IH), 7.65 (m, 2H), 7.35 (m IH), 4.45 (s, 1 H), 4.15 (s, 2H), 3.55 (m, 2H), 3.39 (m, IH), 2.95 (d, IH), 2.35 (s, IH), 2.05 (m, 2H), 1.55 (m, IH); MS (EI) for CnHi6BrN3O3: 390 (MH+). EXAMPLE 28
Figure imgf000272_0001
tert-butyl 2-(fluorocarbonyl)pyrrolidine-l-carboxylate 2
[00589] To a solution of l-(tert-butoxycarbonyl)pyrrolidine-2-carboxylic acid 1 (6.0 g, 27.9 mmol) in dichloromethane (50 mL) at 00C was added pyridine (2.3 mL, 27.9 mmol) and cyanuric fluoride (3.77 g, 31 mmol). The reaction mixture was allowed to warm to room temperature while stirring for 2 h. The reaction was quenched with water (1OmL), and the resulting mixture was filtered through celite and washed with dichloromethane (200 mL). The layers were separated, the organic layer was washed with water (100 mL), brine (100 mL), dried over MgSO4 and concentrated under reduced pressure to afford tert-butyl 2-
(fiuorocarbonyl)pyrrolidine-l-carboxylate 2. 1H NMR (400 MHz, CDCl3): 4.23-4.60 (m, IH), 3.36-3.63 (m, 2H), 1.85-2.43 (m, 4H), 1.42-1.51 (m, 9H). tert-butyl 2-(5 -bromo-2-carbamoylbenzofuran-3 -ylcarbamovDpyrrolidine- 1 - carboxylate 3
[00590] To a solution of 3-amino-5-bromobenzofuran-2-carboxamide 3 (Example 1) (1.5 g, 5.9 mmol) in pyridine (1.5 mL, 17.7 mmol) at 00C was added a solution of tert-butyl 2-(fluorocarbonyl)pyrrolidine-l -carboxylate 2 (1.4 g, 6.5 mmol) in dichloromethane (50 mL) over 15 minutes. The reaction mixture was removed from the ice bath and was stirred over night at room temperature. DMAP (1.0 g, 8.1 mmol) was added and the reaction was again stirred over night at room temperature. The reaction mixture was quenched with water and extracted with ethyl acetate (2 x 150 mL). The combined organic phases were washed with brine, dried over MgSO4 and concentrated under reduced pressure to afford tert-butyl 2-(5-bromo-2- carbamoylbenzofuran-3-ylcarbamoyl)pyrrolidine-l -carboxylate 4. The product was submitted to the next step without further purification. MS (EI) for CIgH22BrNsOs: 452 (MH+). tert-butyl 2-(8-bromo-4-oxo-3,4-dihvdrobenzofuror3,2-dlpyrimidin-2-yl)pyrrolidine- 1-carboxylate 5 [00591] A solution of crude tert-butyl 2-(5-bromo-2-carbamoylbenzofuran-3- ylcarbamoyl)pyrrolidine- 1-carboxylate 4 in ethanol (10 mL) and potassium hydroxide (10%) in water (5 mL) was heated to 800C for 3h. The reaction mixture was cooled down to 0 0C and neutralized to pH 7 with cone. HCl. The precipitate was filtered, washed with ethyl acetate/hexanes and methanol, and dried to afford 1.7 g of tert- butyl 2-(8-bromo-4-oxo-3 ,4-dihydrobenzofuro[3 ,2-d]pyrimidin-2-yl)pyrrolidine- 1 - carboxylate 5 (1.7 g, 66% over 2 steps). MS (EI) for Ci9H20BrN3O4: 434 (MH+).
(Compound 169)
8-bromo-2-pyrrolidin-2-viπibenzofuror3,2-dlpyrimidin-4(3H)-one 6 [00592] A solution of tert-butyl 2-(8-bromo-4-oxo-3,4-dihydrobenzofuro[3,2- d]pyrimidin-2-yl)pyrrolidine- 1-carboxylate 5 (1.7 g, 3.9 mmol) in methanol (10 mL) and 4N HCl in dioxane (5 mL) was stirred overnight at room temperature. The reaction mixture was filtered, resulting in 0.69 g (67%) of the title compound. 1H NMR (400 MHz, d6-DMSO): 10.48-11.50 (br, s, IH), 8.17 (s, IH), 7.76-7.87 (m, 2H), 4.78 (s, IH), 3.43-3.63 (m, 2H), 2.42 (s, IH), 1.91-2.21 (m, 4H); MS (EI) for Ci4Hi2BrN3O2: 334 (MH+).
(Compound 175)
2-(l-aminoethyl)-8-bromoπibenzofuror3,2-dlpyrimidin-4(3H)-one [00593] 2-(l-aminoethyl)-8-bromo[l]benzofuro[3,2-d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 28 wherein l-(tert- butoxycarbonyl)pyrrolidine-2-carboxylic acid was substituted with 2-(tert- butoxycarbonylamino)propanoic acid. 1H NMR (400 MHz, d6-DMSO): 13.47 (s, IH), 8.78 (s, 2H), 8.09 (s, IH), 7.84-7.93 (m, 2H), 4.47 (s, IH), 1.60 (s, 3H); MS (EI) for Ci2Hi0BrN3O2: 308 (MH+).
(Compound 186) 8-bromo-2-pyrrolidin-3-yl[llbenzofuro[3,2-dlpyrimidin-4(3H)-one
[00594] 8-bromo-2-pyrrolidin-3-yl[l]benzofuro[3,2-d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 28 wherein l-(tert- butoxycarbonyl)pyrrolidine-2-carboxylic acid was substituted with l-(tert- butoxycarbony^pyrrolidine-S-carboxylic acid. 1H NMR (400 MHz, dβ-DMSO): 13.17 (s, IH), 9.75 (s, IH), 9.33 (s, IH), 8.27-8.30 (m, IH), 7.79-7.86 (m, 2H), 3.25- 3.84 (m, 4H), 2.31-2.45 (m, IH), 2.15-2.28(m, IH), 2.09 (s, IH), 1.04 (s, IH); MS (EI) for Ci4Hi2BrN3O2: 334 (MH+). (Compound 513)
8-bromo-2-r(2S.4S)-4-fluoropyrrolidin-2-ylirilbenzofuror3.2-dlpyrimidin-4(3H)-one (2S,4S)-tert-butyl 2-(5-bromo-2-carbamoylbenzofuran-3-ylcarbamoyl)-4- fluoropyrrolidine- 1 -carboxylate [00595] The intermediate (2S,4S)-tert-butyl 2-(5-bromo-2-carbamoylbenzofuran-3- ylcarbamoyl)-4-fluoropyrrolidine- 1 -carboxylate was synthesized in a manner similar to 1 , 1 -dimethylethyl 3 -(5 -bromo-2-carbamoylbenzofuran-3 -ylcarbamoyl)azetidine- 1 - carboxylate 4-(tert-butoxycarbonylamino), wherein (2S,4S)-l-(tert-butoxycarbonyl)- 4-fluoropyrrolidine-2-carboxylic acid (commercially available from Omega Chem) replaced Boc-3-azetidine carboxylic acid. The crude material from this reaction was submitted to the next step without any further purification. MS (EI) for C 19 H21 Br F N3 O5 : 471.3 (MH+).
(2S.4S)-tert-butyl 2-(8-bromo-4-oxo-3.4-dihvdrobenzofuror3.2-dlpyrimidin-2-vn-4- fluoropyrrolidine- 1 -carboxylate [00596] (2S.4S)-tert-butyl 2-(8-bromo-4-oxo-3.4-dihvdrobenzofuror3.2- dlpyrimidin-2-yl)-4-fluoropyrrolidine- 1 -carboxylate was synthesized in a similar manner as to 1,1 -dimethylethyl 4-(8-bromo-4-oxo-3,4-dihydrobenzofuro[3,2- d]pyrimidin-2-yl)piperidine-l -carboxylate wherein (2S,4S)-tert-butyl 2-(5-bromo-2- carbamoylbenzofuran-3 -y lcarbamoyl)-4-fluoropyrrolidine- 1 -carboxylate_was substituted for 1,1 -dimethylethyl 4-(5-bromo-2-carbamoylbenzofuran-3- ylcarbamoyl)piperidine-l -carboxylate. MS (EI) for Ci9 Hi9 Br F N3 O4 : 453.3 (MH+).
(Compound 513)
8-bromo-2-r(2S.4S)-4-fluoropyrrolidin-2-ylirilbenzofuror3.2-dlpyrimidin-4(3H)-one
[00597] 8-bromo-2-r(2S.4S)-4-fluoropyrrolidin-2-ylirilbenzofuror3.2-dlpyrimidin- 4(3H)-one was synthesized in a similar manner to 8-bromo-2-piperidin-4- yl[l]benzofuro[3,2-d]pyrimidin-4(3H)-one (Compound 157) wherein 1,1- dimethylethyl 4-(8-bromo-4-oxo-3,4-dihydrobenzofuro[3,2-d]pyrimidin-2- yl)piperidine-l -carboxylate was substituted with (2S,4S)-tert-butyl 2-(8-bromo-4-oxo- 3,4-dihydrobenzofuro[3,2-d]pyrimidin-2-yl)-4-fluoropyrrolidine-l-carboxylate. 1H NMR (400 MHz, d6-DMSO): 8.21 (s, IH), 7.88 (m, 2H), 5.50 (d, IH), 4.98 (dd, IH), 3.80 (m, IH), 3.69 (m, IH), 2.82 (m, 2H), 2.61 (m, IH). MS (EI) for Ci4 Hn Br F N3 O2 : 353.3 (MH+). (Compound 525)
8-chloro-2-r(2S.4S)-4-fluoropyrrolidin-2-ylirilbenzofuror3.2-dlpyrimidin-4(3H)-one (2S,4S)-tert-butyl 2-(2-carbamoyl-5-chlorobenzofuran-3-ylcarbamoyl)-4- fluoropyrrolidine- 1 -carboxylate [00598] The intermediate (2S,4S)-tert-butyl 2-(2-carbamoyl-5-chlorobenzofuran-3- ylcarbamoyl)-4-fluoropyrrolidine- 1 -carboxylate was synthesized in a manner similar to 1 , 1 -dimethylethyl 3 -(5 -bromo-2-carbamoylbenzofuran-3 -ylcarbamoyl)azetidine- 1 - carboxylate 4-(tert-butoxycarbonylamino), wherein (2S,4S)-l-(tert-butoxycarbonyl)- 4-fluoropyrrolidine-2-carboxylic acid (commercially available from Omega Chem) replaced Boc-3-azetidine carboxylic acid. The crude material from this reaction was submitted to the next step without any further purification. MS (EI) for C 19 H21 Cl F N3 O5: 426.8 (MH+).
(2S.4S)-tert-butyl 2-(8-chloro-4-oxo-3.4-dihvdrobenzofuror3.2-dlpyrimidin-2-yl)-4- fluoropyrrolidine- 1 -carboxylate [00599] (2S.4S)-tert-butyl 2-(8-chloro-4-oxo-3.4-dihvdrobenzofuror3.2- dlpyrimidin-2-yl)-4-fluoropyrrolidine- 1 -carboxylate was synthesized in a similar manner as to 1,1 -dimethylethyl 4-(8-bromo-4-oxo-3,4-dihydrobenzofuro[3,2- d]pyrimidin-2-yl)piperidine-l -carboxylate wherein (2S,4S)-tert-butyl 2-(2- carbamoyl-5 -chlorobenzofuran-3 -ylcarbamoyl)-4-fluoropyrrolidine- 1 -carboxylate was substituted for 1,1 -dimethylethyl 4-(5-bromo-2-carbamoylbenzofuran-3- ylcarbamoyl)piperidine-l -carboxylate. MS (EI) for Ci9 Hi9 Cl F N3 O4: 408.8 (MH+).
(Compound 525) 8-chloro-2-r(2S.4S)-4-fluoropyrrolidin-2-ylirilbenzofuror3.2-dlpyrimidin-4(3H)-one
[00600] 8-chloro-2-[(2S,4S)-4-fluoropyrrolidin-2-yl][l]benzofuro[3,2-d]pyrimidin-
4(3H)-one was synthesized in a similar manner to 8-bromo-2-piperidin-4- yl[l]benzofuro[3,2-d]pyrimidin-4(3H)-one (Compound 157) wherein 1,1- dimethylethyl 4-(8-bromo-4-oxo-3,4-dihydrobenzofuro[3,2-d]pyrimidin-2- yl)piperidine-l -carboxylate was substituted with (2S,4S)-tert-butyl 2-(8-chloro-4-oxo-
3,4-dihydrobenzofuro[3,2-d]pyrimidin-2-yl)-4-fluoropyrrolidine-l -carboxylate. 1H NMR (400 MHz, d6-DMSO): 8.06 (d, IH), 7.95 (d, IH), 7.76 (dd, IH), 5.50 (d, IH), 4.97 (dd, IH), 3.80 (m, IH), 3.63 (m, IH), 2.81 (m, 2H), 2.57 (m, IH). MS (EI) for C14 Hn Cl F N3 O2: 308.5 (MH+).
(Compound 526) 8-chloro-2-r(2S.4R)-4-fluoropyrrolidin-2-ylirilbenzofuror3.2-dlpyrimidin-4(3H)-one (2S,4R)-tert-butyl 2-(2-carbamoyl-5-chlorobenzofuran-3-ylcarbamoyl)-4- fluoropyrrolidine- 1 -carboxylate
[00601] The intermediate (2S,4R)-tert-butyl 2-(2-carbamoyl-5-chlorobenzofuran-3- ylcarbamoyl)-4-fluoropyrrolidine-l -carboxylate was synthesized in a manner similar to 1 , 1 -dimethylethyl 3 -(5 -bromo-2-carbamoylbenzofuran-3 -ylcarbamoyl)azetidine- 1 - carboxylate 4-(tert-butoxycarbonylamino), wherein (2S,4R)-l-(tert-butoxycarbonyl)- 4-fluoropyrrolidine-2-carboxylic acid (commercially available from Omega Chem) replaced Boc-3-azetidine carboxylic acid. The crude material from this reaction was submitted to the next step without any further purification. MS (EI) for C19 H2i Cl F N3 O5 : 426.8 (MH+).
(2S.4R)-tert-butyl 2-(8-chloro-4-oxo-3.4-dihvdrobenzofuror3.2-dlpyrimidin-2-vn-4- fluoropyrrolidine- 1 -carboxylate
[00602] (2S,4R)-tert-butyl 2-(8-chloro-4-oxo-3,4-dihydrobenzofuro[3,2- d]pyrimidin-2-yl)-4-fluoropyrrolidine-l -carboxylate was synthesized in a similar manner as to 1,1 -dimethylethyl 4-(8-bromo-4-oxo-3,4-dihydrobenzofuro[3,2- d]pyrimidin-2-yl)piperidine-l -carboxylate wherein (2S,4R)-tert-butyl 2-(2- carbamoyl-5 -chlorobenzofuran-3 -ylcarbamoyl)-4-fluoropyrrolidine- 1 -carboxylate replaced 1,1 -dimethylethyl 4-(5-bromo-2-carbamoylbenzofuran-3- ylcarbamoyl)piperidine-l -carboxylate. MS (EI) for Ci9 H19 Cl F N3 O4 : 408.8 (MH+).
(Compound 526)
8-chloro-2-r(2S.4R)-4-fluoropyrrolidin-2-ylirilbenzofuror3.2-dlpyrimidin-4(3H)-one
[00603] 8-chloro-2-[(2S,4R)-4-fluoropyrrolidin-2-yl][l]benzofuro[3,2- d]pyrimidin-4(3H)-one was synthesized in a similar manner to 8-bromo-2-piperidin- 4-yl[l]benzofuro[3,2-d]pyrimidin-4(3H)-one (Compound 157) wherein 1,1- dimethylethyl 4-(8-bromo-4-oxo-3,4-dihydrobenzofuro[3,2-d]pyrimidin-2- yl)piperidine-l -carboxylate was substituted with (2S,4R)-tert-butyl 2-(8-chloro-4- oxo-3 ,4-dihydrobenzofuro [3 ,2-d]pyrimidin-2-yl)-4-fluoropyrrolidine- 1 -carboxylate. 1H NMR (400 MHz, d6-DMSO): 8.06 (d, IH), 7.95 (d, IH), 7.76 (dd, IH), 5.60 (d, IH), 4.97 (dd, IH), 3.81 (m, IH), 3.67 (m, IH), 2.82 (m, IH), 2.47 (m, 2H). MS (EI) for C14 Hn Cl F N3 O2 : 308.6 (MH+).
(Compound 530) 2-r(2S,4S)-4-fluoropyrrolidin-2-yll-8-(methyloxy)πibenzofuror3,2-dlpyrimidin- 4(3H)-one
(2S,4S)-tert-butyl 2-(2-carbamoyl-5-methoxybenzofuran-3-ylcarbamoyl)-4- fluoropyrrolidine- 1 -carboxylate [00604] The intermediate (2S,4S)-tert-butyl 2-(2-carbamoyl-5-methoxybenzofuran- 3-ylcarbamoyl)-4-fluoropyrrolidine- 1 -carboxylate was synthesized in a manner similar to 1,1-dimethylethyl 3-(5-bromo-2-carbamoylbenzofuran-3- ylcarbamoyl)azetidine-l -carboxylate 4-(tert-butoxycarbonylamino), wherein (2S,4S)- 1 -(tert-butoxycarbonyl)-4-fluoropyrrolidine-2-carboxylic acid (commercially available from Omega Chem) replaced Boc-3-azetidine carboxylic acid. The crude material from this reaction was submitted to the next step without any further purification. MS (EI) for C20 H24 F N3 O6 : 422.4 (MH+). (2S.4S)-tert-butyl 4-fluoro-2-(8-methoxy-4-oxo-3.4-dihvdrobenzofuror3.2- d1pyrimidin-2-yl)pyrrolidine- 1 -carboxylate [00605] (2S,4S)-tert-butyl 4-fluoro-2-(8-methoxy-4-oxo-3,4-dihydrobenzofuro[3,2- d]pyrimidin-2-yl)pyrrolidine-l -carboxylate was synthesized in a similar manner as to 1,1-dimethylethyl 4-(8-bromo-4-oxo-3,4-dihydrobenzofuro[3,2-d]pyrimidin-2- yl)piperidine-l -carboxylate wherein (2S,4S)-tert-butyl 2-(2-carbamoyl-5- methoxybenzofuran-3-ylcarbamoyl)-4-fluoropyrrolidine- 1 -carboxylate replaced 1,1- dimethylethyl 4-(5 -bromo-2-carbamoylbenzofuran-3 -ylcarbamoyl)piperidine- 1 - carboxylate. MS (EI) for C20 H22 F N3 O5 : 404.2 (MH+).
(Compound 530)
2-[(2SΛS)-4-fluoropyrrolidin-2-yll-8-(methyloxy)[llbenzofuro[3,2-dlpyrimidin- 4(3H)-one
[00606] 2-[(2S,4S)-4-fluoropyrrolidin-2-yl]-8-(methyloxy)[l]benzofuro[3,2- d]pyrimidin-4(3H)-one was synthesized in a similar manner to 8-bromo-2-piperidin- 4-yl[l]benzofuro[3,2-d]pyrimidin-4(3H)-one (Compound 157) wherein 1,1- dimethylethyl 4-(8-bromo-4-oxo-3,4-dihydrobenzofuro[3,2-d]pyrimidin-2- yl)piperidine-l -carboxylate was substituted with (2S,4S)-tert-butyl 4-fluoro-2-(8- methoxy-4-oxo-3 ,4-dihydrobenzofuro [3 ,2-d]pyrimidin-2-yl)pyrrolidine- 1 - carboxylate. 1H NMR (400 MHz, d6-DMSO): 7.66 (d, IH), 7.42 (d, IH), 7.18 (dd, IH), 5.21 (d, IH), 3.84 (s, 3H), 3.27 (dd, 2H), 3.01 (dd, IH), 2.23 (m, IH), 1.84 (m, 2H). MS (EI) for Ci5 HM F N3 O3 : 304.0 (MH+). (Compound 500)
8-bromo-2-r(4R)-1.3-thiazolidin-4-ylirilbenzofuror3.2-dlpyrimidin-4(3H)-one (R)-tert-butyl 4-(5 -bromo-2-carbamoylbenzofuran-3 -ylcarbamoyl)thiazolidine-3 - carboxylate [00607] The intermediate (R)-tert-butyl 4-(5-bromo-2-carbamoylbenzofuran-3- ylcarbamoyl)thiazolidine-3 -carboxylate was synthesized in a manner similar to 1,1- dimethylethyl 3 -(5 -bromo-2-carbamoylbenzofuran-3 -ylcarbamoyl)azetidine- 1 - carboxylate 4-(tert-butoxycarbonylamino), wherein (R)-3-(tert- butoxycarbonyl)thiazolidine-4-carboxylic acid (commercially available from Chem- Impex International) replaced Boc-3-azetidine carboxylic acid. The crude material from this reaction was submitted to the next step without any further purification. MS (EI) for C18 H20 Br N3 O5 S: 471.4 (MH+).
(R)-tert-butyl 4-(8-bromo-4-oxo-3,4-dihvdrobenzofuror3,2-dlpyrimidin-2- yl)thiazolidine-3-carboxylate (R)-tert-butyl 4-(8-bromo-4-oxo-3,4-dihydrobenzofuro[3,2-d]pyrimidin-2- yl)thiazolidine-3 -carboxylate was synthesized in a similar manner as to 1,1- dimethylethyl 4-(8-bromo-4-oxo-3,4-dihydrobenzofuro[3,2-d]pyrimidin-2- yl)piperidine-l -carboxylate wherein (R)-tert-butyl 4-(5-bromo-2- carbamoylbenzofuran-3-ylcarbamoyl)thiazolidine-3 -carboxylate replaced 1,1- dimethylethyl 4-(5 -bromo-2-carbamoylbenzofuran-3 -ylcarbamoyl)piperidine- 1 - carboxylate. MS (EI) for Ci8 Hi8 Br N3 O4 S: 453.3 (MH+).
(Compound 500)
8-bromo-2-r(4R)-1.3-thiazolidin-4-ylirilbenzofuror3.2-dlpyrimidin-4(3H)-one
[00608] 8-bromo-2-[(4R)-l,3-thiazolidin-4-yl][l]benzofuro[3,2-d]pyrimidin-
4(3H)-one was synthesized in a similar manner to 8-bromo-2-piperidin-4- yl[l]benzofuro[3,2-d]pyrimidin-4(3H)-one (Compound 157) wherein 1,1- dimethylethyl 4-(8-bromo-4-oxo-3,4-dihydrobenzofuro[3,2-d]pyrimidin-2- yl)piperidine-l -carboxylate was substituted with (R)-tert-butyl 4-(8-bromo-4-oxo-3,4- dihydrobenzofuro[3,2-d]pyrimidin-2-yl)thiazolidine-3-carboxylate. 1H NMR (400 MHz, de-DMSO): 8.21 (s, IH), 7.88 (m, 2H), 4.90 (m, 2H), 4.57 (m, 2H), 4.40 (m, 2H). MS (EI) for Ci3 Hi0 Br N3 O2 S: 353.3 (MH+).
(Compound 514)
8-bromo-2-r(2S.4R)-4-fluoropyrrolidin-2-ylirilbenzofuror3.2-dlpyrimidin-4(3H)-one (2R,4S)-tert-butyl 2-(5-bromo-2-carbamoylbenzofuran-3-ylcarbamoyl)-4- fluoropyrrolidine- 1 -carboxylate
[00609] The intermediate (2R,4S)-tert-butyl 2-(5-bromo-2-carbamoylbenzofuran- 3 -ylcarbamoyl)-4-fluoropyrrolidine-l -carboxylate was synthesized in a manner similar to 1,1-dimethylethyl 3-(5-bromo-2-carbamoylbenzofuran-3- ylcarbamoyl)azetidine- 1 -carboxylate 4-(tert-butoxycarbonylamino), wherein (2S,4R)- 1 -(tert-butoxycarbonyl)-4-fluoropyrrolidine-2-carboxylic acid (N-t-BOC-trans-4- Fluoro-L-Proline, OmegaChem Inc.) replaced Boc-3-azetidine carboxylic acid. The crude material from this reaction was submitted to the next step without any further purification. MS (EI) for C19 H21 B rF N3 O5: 471.3 (MH+). (2R.4S)-tert-butyl 2-(8-bromo-4-oxo-3.4-dihvdrobenzofuror3.2-dlpyrimidin-2-vn-4- fluoropyrrolidine- 1 -carboxylate
[00610] (2R,4S)-tert-Butyl 2-(8-bromo-4-oxo-3,4-dihydrobenzofuro[3,2- d]pyrimidin-2-yl)-4-fluoropyrrolidine-l-carboxylate_was synthesized in a similar manner as to 1,1-dimethylethyl 4-(8-bromo-4-oxo-3,4-dihydrobenzofuro[3,2- d]pyrimidin-2-yl)piperidine-l -carboxylate wherein (2R,4S)-tert-butyl 2-(5-bromo-2- carbamoylbenzofuran-3 -ylcarbamoyl)-4-fluoropyrrolidine- 1 -carboxylate replaced 1,1- dimethylethyl 4-(5 -bromo-2-carbamoylbenzofuran-3 -ylcarbamoyl)piperidine- 1 - carboxylate. MS (EI) for Ci9 H19 Br F N3 O4: 452.2 (MH+). 8-bromo-2-r(2S.4R)-4-fiuoropyrrolidin-2-ylirilbenzofuror3.2-dlpyrimidin-4(3H)-one [00611] 8-bromo-2-[(2S,4R)-4-fluoropyrrolidin-2-yl][l]benzofuro[3,2- d]pyrimidin-4(3H)-one was synthesized in a similar manner to 8-bromo-2-piperidin- 4-yl[l]benzofuro[3,2-d]pyrimidin-4(3H)-one (Compound 157) wherein 1,1- dimethylethyl 4-(8-bromo-4-oxo-3,4-dihydrobenzofuro[3,2-d]pyrimidin-2- yl)piperidine-l -carboxylate was substituted with (2R,4S)-tert-butyl 2-(8-bromo-4- oxo-3 ,4-dihydrobenzofuro [3 ,2-d]pyrimidin-2-yl)-4-fluoropyrrolidine- 1 -carboxylate. 1H NMR (400 MHz, d6-DMSO): 8.21 (s, IH), 7.89 (m, 2H), 5.61 (d, IH), 4.98 (m, IH), 3.73 (m, 2H), 2.84 (m, IH), 2.45 (m, H). MS (EI) for C14 Hn Br F N3 O2 352.0 (MH+). (Compound 505)
8-bromo-2-r(2S)-2.5-dihvdro-lH-pyrrol-2-ylirilbenzofuror3.2-dlpyrimidin-4(3H)- one
(S)-tert-butyl 2-(5-bromo-2-carbamoylbenzofuran-3-ylcarbamoyl)-2,5-dihydro-lH- pyrrole- 1 -carboxylate
[00612] The intermediate (S)-tert-butyl 2-(5-bromo-2-carbamoylbenzofuran-3- ylcarbamoyl)-2,5-dihydro-lH-pyrrole-l-carboxylate was synthesized in a manner similar to 1,1-dimethylethyl 3-(5-bromo-2-carbamoylbenzofuran-3- ylcarbamoyl)azetidine-l -carboxylate 4-(tert-butoxycarbonylamino), wherein (S)-I- (tert-butoxycarbonyl^^-dihydro-lH-pyrrole^-carboxylic acid (BOC-3,4, dehydro-
L-proline, NeoMPS) replaced Boc-3-azetidine carboxylic acid. The crude material from this reaction was submitted to the next step without any further purification. MS
(EI) for Ci9 H20 Br N3 O5: 451.3 (MH+).
(S)-tert-butyl 2-(8-bromo-4-oxo-3,4-dihydrobenzofuro[3,2-dlpyrimidin-2-yl)-2,5- dihydro- 1 H-pyrrole- 1 -carboxylate
[00613] (S)-tert-butyl 2-(8-bromo-4-oxo-3,4-dihydrobenzofuro[3,2-d]pyrimidin-2- yl)-2,5-dihydro-lH-pyrrole-l-carboxylate was synthesized in a similar manner as to
1,1-dimethylethyl 4-(8-bromo-4-oxo-3,4-dihydrobenzofuro[3,2-d]pyrimidin-2- yl)piperidine-l -carboxylate wherein (S)-tert-butyl 2-(2-(aminomethyl)-5- bromobenzofuran-3-ylcarbamoyl)-2,5-dihydro-lH-pyrrole-l -carboxylate replaced
1 , 1 -dimethylethyl 4-(5-bromo-2-carbamoylbenzofuran-3-ylcarbamoyl)piperidine- 1 - carboxylate. MS (EI) for Ci9 Hi8 Br N3 O4 : 433 (MH+).
8-bromo-2-r(2S)-2.5-dihvdro-lH-pyrrol-2-ylirilbenzofuror3.2-dlpyrimidin-4(3H)- one 8-bromo-2-[(2S)-2,5-dihydro-lH-pyrrol-2-yl][l]benzofuro[3,2-d]pyrimidin-4(3H)- one was synthesized in a similar manner to 8-bromo-2-piperidin-4- yl[l]benzofuro[3,2-d]pyrimidin-4(3H)-one (Compound 157) wherein 1,1- dimethylethyl 4-(8-bromo-4-oxo-3,4-dihydrobenzofuro[3,2-d]pyrimidin-2- yl)piperidine-l -carboxylate was substituted with (S)-tert-butyl 2-(8-bromo-4-oxo-3,4- dihydrobenzofuro[3,2-d]pyrimidin-2-yl)-2,5-dihydro-lH-pyrrole-l -carboxylate.1H
NMR (400 MHz, d6-DMSO): 8.22 (s, IH), 7.88 (m, 2H), 6.16 (d, IH), 5.54 (s, IH), 4.31 (d, IH), 4.14 (d, IH). MS (EI) for Ci4 Hi0 Br N3 O2: 331.9 (MH+).(Compound 527) 8-Bromo-2-r(2S)-l-methylpyrrolidin-2-ylirHbenzofuror3.2-d1pyrimidin-4(3H)-one hydrochloride
Λ/-r2-(aminocarbonyl)-5-bromo- 1 -benzofuran-3-vH-L-prolinamide hydrochloride [00614] l,l-Dimethylethyl (25)-2-({[2-(aminocarbonyl)-5-bromo-l-benzofuran-3- yl] amino } carbonyl)pyrrolidine- 1 -carboxylate (intermediate from Compound 470 synthesis) (1000 mg, 2.22 mmol) was suspended in dioxane (4 mL). A solution of 4
M hydrochloric acid in dioxane (8 mL) was added and the reaction was stirred for 7 hr at room temperature. The reaction mixture was filtered and the precipitate was washed with dioxane (2 mL). 580 mg of product was isolated after drying in air. MS (EI) for Ci4Hi4BrN3O3: 352 (MH+).
Λ/-r2-(aminocarbonyl)-5-bromo- 1 -benzofuran-3-vH- 1 -methyl-L-prolinamide [00615] Λ/-[2-(aminocarbonyl)-5-bromo- 1 -benzofuran-3-yl]-L-prolinamide hydrochloride (580 mg, 1.49 mmol) was dissolved in dimethylformamide (8 mL).
37% Aqueous formaldehyde (1.0 mL) was added followed by sodium triacetoxyborohydride (630 mg, 2.97 mmol). The reaction mixture was stirred for 10 min at room temperature, then IM hydrochloric acid was added to lower the pH to 2.
The reaction mixture was diluted with water (10 mL) and IM sodium hydroxide was added to increase the pH to 8. The precipitate was filtered off, washed with water (5 mL) and air-dried to give 215 mg of white powder. MS (EI) for CiSHIeBrN3O3: 366 (MH+).
8-Bromo-2-r(2S)-l-methylpyrrolidin-2-ylirilbenzofuror3.2-d1pyrimidin-4(3H)-one hydrochloride
[00616] N- [2-(Aminocarbonyl)-5 -bromo- 1 -benzofuran-3 -yl] - 1 -methyl-L- prolinamide was suspended in ethanol (3.6 mL). Sodium hydroxide (1.0 M, 1.8 mL) was added and the reaction mixture was heated at 80 0C for 8 hr. After cooling to room temperature, the reaction mixture was acidified with 1 M hydrochloric acid to pH 3. The precipitate was filtered off and washed with water (2 mL) and acetonitrile (1 mL). 86 mg of product was isolated after drying under air. 1H NMR (400 MHz, de-DMSO): 13.49 (br s, IH), 10.11 (br s, IH), 8.20 (s, IH), 7.89 (d, IH), 7.85 (dd, IH), 4.55 (m, IH), 3.74 (m, IH), 2.97 (s, 3H), 2.65 (m, IH), 2.1-2.0 (m, 3H). MS (EI) for Ci5Hi4BrN3O2: 348 (MH+).
(Compound 49)
2-(l-Amino-l-methylethyl)-8-chloro[llbenzofuro[3,2-dlpyrimidin-4(3H)-one 9H-Fluoren-9-ylmethyl (2- { |"2-(aminocarbonyl)-5 -chloro- 1 -benzofuran-3 -yl] amino I - 1 , 1 -dimethyl-2-oxoethyπcarbamate
[00617] N-{[(9H-Fluoren-9-ylmethyl)oxy]carbonyl}-2-methylalanine (15.0 g, 46.1 mmol) was suspended in dichloromethane (200 ml). The reaction was stirred at room temperature overnight. The mixture was concentrated to dryness under vacuum. The residue was taken up in dichloromethane (25 mL) and added to another solution of 3- amino-5 -chloro- l-benzofuran-2-carboxamide (1950 mg, 9.26 mmol) in pyridine (50 mL). The reaction mixture was stirred at room temperature overnight. The precipitate was filtered off and washed with pyridine (5 mL) and ethyl acetate (2 mL) to give 6.4 g of crude product. 1H NMR (400 MHz, d6-DMSO): 10.59 (s, IH), 8.29 (d, IH), 8.16 (s, IH), 8.01 (s, IH), 7.91 (s, IH), 7.86 (d, 2H), 7.71 (d, 2H), 7.60 (d, IH), 7.51 (dd, IH), 7.38 (m, 2H), 7.28 (m, IH), 4.26 (m, 2H), 4.21 (m, IH), 1.44 (s, 6H). MS (EI) for C26H24ClN3O5: 518 (MH+). 2-(l-Amino-l-methylethyl)-8-chloro[llbenzofuro[3,2-dlpyrimidin-4(3H)-one [00618] 9H-Fluoren-9-ylmethyl (2-{[2-(aminocarbonyl)-5-chloro-l-benzofuran-3- yl] amino }-l,l-dimethyl-2-oxoethyl)carbamate (crude , 9.3 mmol) was suspended in ethanol (50 mL) and sodium hydroxide (2.0 M, 25 mL) was added. The reaction was stirred at 100 0C overnight. After cooling, the reaction mixture was diluted with water (25 mL) and washed twice with ethyl acetate (25 mL). The aqueous fraction was acidified with concentrated hydrochloric acid to pH 6. The precipitate was filtered off and washed with water (2 mL) and acetonitrile (2 mL). 625 mg of white solid was isolated after drying under vacuum. 1H NMR (400 MHz, d6-DMSO): 7.93 (s, IH), 7.87 (d, IH), 7.68 (dd, IH), 1.67 (s, 6H). MS (EI) for Ci3Hi2ClN3O2: 278 (MH+). Synthesis of S-Amino-S-chloro-l-benzofuran-l-carboxamide 5 -Chloro-2-hydroxybenzaldehyde oxime
[00619] 5-Chlorosalicylaldehyde (1) (10 g, 63.90 mmol) was stirred in isopropyl alcohol (30 mL) to form a thick slurry. 50% Aqueous hydroxylamine (10 mL, 151 mmol), was added to the reaction mixture over the course of 5 minutes, then the suspension was heated to 900C. After 4 h, the insoluble material dissolved and the reaction mixture was stirred at 900C overnight. The reaction was allowed to cool to room temperature, which caused a white precipitate to form. The reaction mixture was poured into ice water (100 ml crushed ice, 50 ml water). The white slurry was stirred continually for 5 minutes and was allowed to stand for 1 hour with occasional stirring. The solid was isolated by filtration, washed with cold water (4 x 5 mL) and dried under reduced pressure for 24 h to give 10.87 g (99% yield, >95% purity) of 5-chloro- 2-hydroxybenzaldehyde oxime. The material was used without further purification. 1H NMR (400 MHz, d6-DMSO): 11.49 (br s,lH), 10.29 (br s, IH), 8.25 (s, IH), 7.49 (d, IH), 7.23 (dd, IH), 6.86 (d, IH). MS (EI) for C7H6ClNO2: 172 (MH+).
5 -Chloro-2-hvdroxybenzonitrile
[00620] 5-Chloro-2-hydroxybenzaldehyde oxime (6. H g, 35.60 mmol) was dissolved in anhydrous N, N-dimethylformamide (3000 mL) under a nitrogen atmosphere. The reaction mixture was cooled in an ice bath to lower the internal temperature to -100C. Phosphorus oxychloride (8.30 mL, 89 mmol) was added dropwise to the cold reaction mixture over 6 h to maintain the temperature below 00C. The reaction mixture was allowed to stir overnight as the cold bath slowly warmed to room temperature. The resulting slurry was then poured into a stirred mixture of ice water (100 ml crushed ice, 50 ml water). The aqueous suspension was allowed to stand at room temperature overnight. The solid was collected by filtration, washed with cold water (4 x 10 mL) and dried under reduced pressure for 24 h to give 4.64 g of 5-chloro-2-hydroxybenzonitrile (85% yield, >90% purity). The material was used without further purification. 1H NMR (400 MHz, d6-DMSO): 8.28 (s, IH), 7.53 (s, IH), 7.22 (d, IH), 6.91 (d, IH). MS (EI) for C7H4ClNO: 154 (MH+). 2-(4-Chloro-2-cyanophenoxy)acetamide
[00621] 5-Chloro-2-hydroxybenzonitrile (4.60 g, 30 mmol) and 2-chloroacetamide (4.03 g, 43.10 mol) were dissolved in anhydrous N, N-dimethylacetamide 50 mL). Cesium carbonate (12.70 g, 38.9 ,mol) was added in portions over 5 min and the reaction mixture was stirred at 800C overnight. After cooling to room temperature, the reaction mixture was poured into a mixture of ice water (100 mL crushed ice, 50 mL water). The solid was isolated by filtration, washed with cold water (4 x 10 mL) and air dried for 48 hours to give 5.36 g (85% yield, >90% purity) of 2-(4-chloro-2- cyanophenoxy)acetamide. The material was used without further purification. 1H NMR (400 MHz, d6-DMSO): 7.91 (dd, IH), 7.69 (dd, IH), 7.51 (br s, IH), 7.44 (br s, IH), 7.07 (d, IH), 4.68 (s, 2H). MS (EI) for C9H7ClN2O2: 211 (MH+).
3 - Amino-5 -chloro- 1 -benzofuran-2-carboxamide [00622] Potassium hydroxide (1.83 g, 32.6 mmol) was dissolved in isopropyl alcohol (40 rnL) at 500C. The solution was diluted with more isopropyl alcohol (10 mL), then 2-(4-chloro-2-cyanophenoxy)acetamide (3.43 g, 16.3 mol) was added followed by more isopropyl alcohol (40 mL). The suspension was stirred at 900C for 8 hours. After cooling to room temperature, the suspension was poured into a stirred mixture of ice water (100 ml ice, 50 mL water) and allowed to stand for 1 h. The precipitate was collected by filtration, washed with cold water (4 x 10 mL) and air dried for 72 h to give 309 g (90% yield, >95% purity) of 3-amino-5-chloro-l- benzofuran-2-carboxamide. The material was used without further purification. 1H NMR (400 MHz, d6-DMSO): 7.94 (d, IH), 7.42 (d, 2H), 7.34 (br s, 2H), 6.01 (br s, 2H). MS (EI) for C9H7ClN2O2: 211 (MH+).
(Compound 511)
8-bromo-2-r(2tS'.4^-4-hvdroxypyrrolidin-2-ylirilbenzofuror3.2-dlpyrimidin-4(3H)- one (2RAS)-tert-butγ\ 2-(5 -bromo-2-carbamoylbenzofuran-3 -ylcarbamoyl)-4- hydroxypyrrolidine- 1 -carboxylate
[00623] The intermediate (2R,4S)-tert-buty\ 2-(5-bromo-2-carbamoylbenzofuran-3- ylcarbamoyl)-4-hydroxypyrrolidine-l -carboxylate was synthesized in a manner similar to 1,1-dimethylethyl 3-(5-bromo-2-carbamoylbenzofuran-3- ylcarbamoyl)azetidine- 1 -carboxylate 4-(tert-butoxycarbonylamino), wherein N-boc- cώ-4-hydroxypyrrolidine-2-carboxylic acid (commercially available from Omega Chem, Canada) replaced Boc-3-azetidine carboxylic acid. The crude material from this reaction was submitted to the next step without any further purification. MS (EI) for C19 H22 Br N3 O6: 469 (MH+).
Figure imgf000284_0001
hydroxypyrrolidine- 1 -carboxylate
[00624] (25f,45)-fert-butyl 2-(8-bromo-4-oxo-3,4-dihydrobenzofuro[3,2- <i]pyrimidin-2-yl)-4-hydroxypyrrolidine-l-carboxylate_was synthesized in a similar manner as to 1,1-dimethylethyl 4-(8-bromo-4-oxo-3,4-dihydrobenzofuro[3,2- d]pyrimidin-2-yl)piperidine-l -carboxylate wherein (2R,4S)-tert-buty{ 2-(5-bromo-2- carbamoylbenzofuran-3-ylcarbamoyl)-4-hydroxypyrrolidine- 1 -carboxylate replaced 1 , 1 -dimethylethyl 4-(5-bromo-2-carbamoylbenzofuran-3-ylcarbamoyl)piperidine- 1 - carboxylate. MS (EI) for Ci9 H20 Br N3 O5: 451 (MH+). 8-bromo-2-r(2S.4S)-4-hvdroxypyrrolidin-2-ylirilbenzofuror3.2-dlpyrimidin-4(3H)- one
[00625] 8-bromo-2-[(2S,4S)-4-hydroxypyrrolidin-2-yl][l]benzofuro[3,2- d]pyrimidin-4(3H)-one was synthesized in a similar manner to 8-bromo-2-piperidin- 4-yl[l]benzofuro[3,2-d]pyrimidin-4(3H)-one (Compound 157) wherein 1,1- dimethylethyl 4-(8-bromo-4-oxo-3,4-dihydrobenzofuro[3,2-d]pyrimidin-2- yl)piperidine-l-carboxylate was substituted with (2S,4S)-tert-buty\ 2-(8-bromo-4-oxo- 3,4-dihydrobenzofuro[3,2-(i]pyrimidin-2-yl)-4-hydroxypyrrolidine-l-carboxylate. 1H NMR (400 MHz, d6-DMSO): 10.56 (s, br, IH), 9.18 (s, br, IH), 8.21 (m, IH), 7.88 (M, 2H), 5.45 (s, br, IH), 4.84 (m, IH), 4.48 (m, IH), 3.35 (m, 2H), 2.64 (m, IH), 2.22 (m, IH). MS (EI) for Ci4 H12 Br N3 O3: 352 (MH+).
(Compound 512)
8-bromo-2-r(2S)-4.4-difluoropyrrolidin-2-ylirilbenzofuror3.2-dlpyrimidin-4(3H)-one (R)-tert-butyl 2-(5-bromo-2-carbamoylbenzofuran-3-ylcarbamoyl)-4,4 difluoropyrrolidine- 1 -carboxylate
[00626] The intermediate (R)-tert-butyl 2-(5-bromo-2-carbamoylbenzofuran-3- ylcarbamoyl)-4,4-difluoropyrrolidine-l -carboxylate was synthesized in a manner similar to 1,1-dimethylethyl 3-(5-bromo-2-carbamoylbenzofuran-3- ylcarbamoyl)azetidine-l -carboxylate 4-(tert-butoxycarbonylamino), wherein (R)-I- (tert-butoxycarbonyl)-4,4-difluoropyrrolidine-2-carboxylic acid (commercially available from OMEGACHEM, INC.) replaced Boc-3-azetidine carboxylic acid. The crude material from this reaction was submitted to the next step without any further purification. MS (EI) for Ci9H20BrF2N3O5: 489.3 (MH+). (R)-tert-butyl 2-(8-bromo-4-oxo-3,4-dihvdrobenzofuror3,2-dlpyrimidin-2-yl)-4,4- difluoropyrrolidine- 1 -carboxylate
[00627] (R)-tert-butyl 2-(8-bromo-4-oxo-3,4-dihydrobenzofuro[3,2-d]pyrimidin-2- yl)-4,4-difluoropyrrolidine-l -carboxylate was synthesized in a similar manner as to 1,1-dimethylethyl 4-(8-bromo-4-oxo-3,4-dihydrobenzofuro[3,2-d]pyrimidin-2- yl)piperidine-l -carboxylate wherein (R)-tert-butyl 2-(5-bromo-2- carbamoylbenzofuran-3-ylcarbamoyl)-4,4-difluoropyrrolidine-l -carboxylate replaced 1 , 1 -dimethylethyl 4-(5-bromo-2-carbamoylbenzofuran-3-ylcarbamoyl)piperidine- 1 - carboxylate. MS (EI) for Ci9Hi8BrF2N3O4: 471.3 (MH+).
8-bromo-2-[(2S)-4,4-difluoropyrrolidin-2-yll[llbenzofuro[3,2-dlpyrimidin-4(3H)-one [00628] 8-bromo-2-[(2S)-4,4-difluoropyrrolidin-2-yl][l]benzofuro[3,2- d]pyrimidin-4(3H)-one was synthesized in a similar manner to 8-bromo-2-piperidin-4-yl[l]benzofuro[3,2- d]pyrimidin-4(3H)-one (Compound 157) wherein 1,1-dimethylethyl 4-(8-bromo-4- oxo-3 ,4-dihydrobenzofuro [3 ,2-d]pyrimidin-2-yl)piperidine- 1 -carboxylate was substituted with (R)-tert-butyl 2-(8-bromo-4-oxo-3,4-dihydrobenzofuro[3,2- d]pyrimidin-2-yl)-4,4-difluoropyrrolidine-l -carboxylate. 1H NMR (400 MHz, d6- DMSO): 8.22 (s, IH), 7.88 (m, 2H), 5.14 (t, IH), 4.03 (m, IH), 3.89 (m, IH), 3.13 (m, IH), 2.93 (m, IH). MS (EI) for Ci4Hi0BrF2N3O2 : 371.2 (MH+). (Compound 524)
8-chloro-2-r(2S)-4,4-difluoropyrrolidin-2-ylirilbenzofuror3,2-dlpyrimidin-4(3H)-one (R)-tert-butyl 2-(2-carbamoyl-5-chlorobenzofuran-3-ylcarbamoyl)-4,4- difluoropyrrolidine- 1 -carboxylate [00629] The intermediate (R)-tert-butyl 2-(2-carbamoyl-5-chlorobenzofuran-3- ylcarbamoyl)-4,4-difluoropyrrolidine-l -carboxylate was synthesized in a manner similar to 1,1-dimethylethyl 3-(5-bromo-2-carbamoylbenzofuran-3- ylcarbamoyl)azetidine-l -carboxylate 4-(tert-butoxycarbonylamino), wherein (R)-I- (tert-butoxycarbonyl)-4,4-difluoropyrrolidine-2-carboxylic acid (commercially available from OMEGACHEM, INC.) replaced Boc-3-azetidine carboxylic acid. The crude material from this reaction was submitted to the next step without any further purification. MS (EI) for Ci9H20ClF2N3O5: 444.1 (MH+).
(R)-tert-butyl 2-(8-chloro-4-oxo-3.4-dihvdrobenzofuror3.2-dlpyrimidin-2-vn-4.4- difluoropyrrolidine- 1 -carboxylate [00630] (R)-tert-butyl 2-(8-chloro-4-oxo-3,4-dihydrobenzofuro[3,2-d]pyrimidin-2- yl)-4,4-difluoropyrrolidine-l -carboxylate was synthesized in a similar manner as to 1,1-dimethylethyl 4-(8-bromo-4-oxo-3,4-dihydrobenzofuro[3,2-d]pyrimidin-2- yl)piperidine-l -carboxylate wherein (R)-tert-butyl 2-(2-carbamoyl-5- chlorobenzofuran-3-ylcarbamoyl)-4,4-difluoropyrrolidine-l -carboxylate replaced 1,1- dimethylethyl 4-(5 -bromo-2-carbamoylbenzofuran-3 -ylcarbamoyl)piperidine- 1 - carboxylate. MS (EI) for Ci9Hi8ClF2N3O4: 426.1 (MH+).
8-chloro-2-r(2S)-4.4-difluoropyrrolidin-2-ylirilbenzofuror3.2-d1pyrimidin-4(3H)-one
[00631] 8-chloro-2-[(2S)-4,4-difluoropyrrolidin-2-yl][l]benzofuro[3,2- d]pyrimidin-4(3H)-one was synthesized in a similar manner to 8-bromo-2-piperidin- 4-yl[l]benzofuro[3,2-d]pyrimidin-4(3H)-one (Compound 157) wherein 1,1- dimethylethyl 4-(8-bromo-4-oxo-3,4-dihydrobenzofuro[3,2-d]pyrimidin-2- yl)piperidine-l-carboxylate was substituted with (R)-tert-butyl 2-(8-chloro-4-oxo-3,4- dihydrobenzofuro[3,2-d]pyrimidin-2-yl)-4,4-difluoropyrrolidine-l-carboxylate. 1H NMR (400 MHz, d6-DMSO): 8.09 (s, IH), 7.95 (s, IH), 7.77 (d, IH), 5.12 (t, IH), 3.95 (m, 2H), 3.12 (m, IH), 2.93 (m, IH). MS (EI) for CI4HI0CIF2N3O2: 326.0 (MH+).
(Compound 528)
8-chloro-2-r(2S.4S)-4-hvdroxypyrrolidin-2-ylirilbenzofuror3.2-dlpyrimidin-4(3H)- one
(2S,4S)-tert-butyl 2-(2-carbamoyl-5-chlorobenzofuran-3-ylcarbamoyl)-4- hydroxypyrrolidine- 1 -carboxylate
[00632] The intermediate (2S,4S)-tert-butyl 2-(2-carbamoyl-5-chlorobenzofuran-3- ylcarbamoyl)-4-hydroxypyrrolidine-l -carboxylate was synthesized in a manner similar to 1 , 1 -dimethylethyl 3 -(5 -bromo-2-carbamoylbenzofuran-3 - ylcarbamoyl)azetidine-l -carboxylate 4-(tert-butoxycarbonylamino), wherein (2S,4S)- 4-hydroxypyrrolidine-2-carboxylic acid (commercially available from OMEGACHEM, INC.) replaced Boc-3-azetidine carboxylic acid. The crude material from this reaction was submitted to the next step without any further purification. MS (EI) for Ci9H22ClN3O6: 424.1 (MH+).
(2S.4S)-tert-butyl 2-(8-chloro-4-oxo-3.4-dihvdrobenzofuror3.2-d1pyrimidin-2-yl)-4- hvdroxypyrrolidine- 1 -carboxylate
[00633] (2S,4S)-tert-butyl 2-(8-chloro-4-oxo-3,4-dihydrobenzofuro[3,2- d]pyrimidin-2-yl)-4-hydroxypyrrolidine-l -carboxylate was synthesized in a similar manner as to 1,1 -dimethylethyl 4-(8-bromo-4-oxo-3,4-dihydrobenzofuro[3,2- d]pyrimidin-2-yl)piperidine-l -carboxylate wherein (2S,4S)-tert-butyl 2-(2- carbamoyl-5 -chlorobenzofuran-3 -ylcarbamoyl)-4-hydroxypyrrolidine- 1 -carboxylate replaced 1,1 -dimethylethyl 4-(5-bromo-2-carbamoylbenzofuran-3- ylcarbamoyl)piperidine-l -carboxylate. MS (EI) for Ci9H20ClN3O5: 406.1 (MH+). 8-chloro-2-r(2S.4S)-4-hvdroxypyrrolidin-2-ylirilbenzofuror3.2-dlpyrimidin-4(3H)- one
[00634] 8-chloro-2-[(2S,4S)-4-hydroxypyrrolidin-2-yl][l]benzofuro[3,2- d]pyrimidin-4(3H)-one was synthesized in a similar manner to 8-bromo-2-piperidin- 4-yl[l]benzofuro[3,2-d]pyrimidin-4(3H)-one (Compound 157) wherein 1,1- dimethylethyl 4-(8-bromo-4-oxo-3,4-dihydrobenzofuro[3,2-d]pyrimidin-2- yl)piperidine-l-carboxylate was substituted with (2S,4S)-tert-butyl 2-(8-chloro-4-oxo- 3,4-dihydrobenzofuro[3,2-d]pyrimidin-2-yl)-4-hydroxypyrrolidine-l-carboxylate. 1H NMR (400 MHz, d6-DMSO): 8.01 (s, IH), 7.83 (d, IH), 7.64 (d, IH), 4.33 (s, IH), 3.47 (m, IH), 3.06 (m, 2H), 2.43 (m, IH), 2.01 (m, IH). MS (EI) for Ci4Hi2ClN3O3 : 306.1 (MH+).
(Compound 529)
8-chloro-2-r(2S)-octahvdro-lH-indol-2-ylirilbenzofuror3.2-dlpyrimidin-4(3H)-one (2S)-tert-butyl 2-(2-carbamoyl-5-chlorobenzomran-3-ylcarbamoyl)octahvdro-lH- indole- 1 -carboxylate
[00635] The intermediate (2S)-tert-butyl 2-(2-carbamoyl-5-chlorobenzofuran-3- ylcarbamoyl)octahydro-lH-indole-l -carboxylate was synthesized in a manner similar to 1 , 1 -dimethylethyl 3 -(5 -bromo-2-carbamoylbenzofuran-3 -ylcarbamoyl)azetidine- 1 - carboxylate 4-(tert-butoxycarbonylamino), wherein (2S)-l-(tert- butoxycarbonyl)octahydro-lH-indole-2-carboxylic acid (commercially available from OMEGACHEM, INC.) replaced Boc-3-azetidine carboxylic acid. The crude material from this reaction was submitted to the next step without any further purification. MS (EI) for C23H28ClN3O5: 462.2 (MH+). (2S)-tert-butyl 2-(8-chloro-4-oxo-3,4-dihydrobenzofuro[3,2-dlpyrimidin-2- vDoctahvdro- 1 H-indole- 1 -carboxylate
[00636] (2S)-tert-butyl 2-(8-chloro-4-oxo-3,4-dihydrobenzofuro[3,2-d]pyrimidin- 2-yl)octahydro-l H-indole- 1 -carboxylate was synthesized in a similar manner as to 1,1 -dimethylethyl 4-(8-bromo-4-oxo-3,4-dihydrobenzofuro[3,2-d]pyrimidin-2- yl)piperidine-l -carboxylate wherein (2S)-tert-butyl 2-(2-carbamoyl-5- chlorobenzofuran-3 -ylcarbamoyl)octahydro- 1 H-indole- 1 -carboxylate replaced 1,1- dimethylethyl 4-(5 -bromo-2-carbamoylbenzofuran-3 -ylcarbamoyl)piperidine- 1 - carboxylate. MS (EI) for C23H26ClN3O4: 444.2 (MH+). 8-chloro-2-r(2S)-octahvdro-lH-indol-2-yliπibenzofuror3,2-dlpyrimidin-4(3H)-one [00637] 8-chloro-2-[(2S)-octahydro-lH-indol-2-yl][l]benzofuro[3,2-d]pyrimidin- 4(3H)-one was synthesized in a similar manner to 8-bromo-2-piperidin-4- yl[l]benzofuro[3,2-d]pyrimidin-4(3H)-one (Compound 157) wherein 1,1- dimethylethyl 4-(8-bromo-4-oxo-3,4-dihydrobenzofuro[3,2-d]pyrimidin-2- yl)piperidine-l-carboxylate was substituted with (2S)-tert-butyl 2-(8-chloro-4-oxo- 3 ,4-dihydrobenzofuro[3 ,2-d]pyrimidin-2-yl)octahydro- 1 H-indole- 1 -carboxylate. 1H NMR (400 MHz, d6-DMSO): 8.07 (s, IH), 7.92 (d, IH), 7.73 (d, IH), 4.76 (t, IH), 3.70 (m, IH), 2.48 (m, IH), 2.78 (m, IH), 1.92 (s, IH), 1.64 (m, 4H), 1.34 (m, 4H). MS (EI) for Ci8Hi8ClN3O2: 344.1 (MH+).
(Compound 531)
2-[(2S)-4,4-difluoropyrrolidin-2-yll-8-(methyloxy)[llbenzofuro[3,2-dlpyrimidin-
4(3H)-one
(R)-tert-butyl 2-(2-carbamoyl-5-methoxybenzofuran-3-ylcarbamoyl)-4,4- difluoropyrrolidine- 1 -carboxylate
[00638] The intermediate (R)-tert-butyl 2-(2-carbamoyl-5-methoxybenzofuran-3- ylcarbamoyl)-4,4-difluoropyrrolidine-l -carboxylate was synthesized in a manner similar to 1,1-dimethylethyl 3-(5-bromo-2-carbamoylbenzofuran-3- ylcarbamoyl)azetidine-l -carboxylate 4-(tert-butoxycarbonylamino), wherein (R)-I- (tert-butoxycarbonyl)-4,4-difluoropyrrolidine-2-carboxylic acid (commercially available from OMEGACHEM, INC.) replaced Boc-3-azetidine carboxylic acid. The crude material from this reaction was submitted to the next step without any further purification. MS (EI) for C20H23F2N3O6: 440.2 (MH+).
(R)-tert-butyl 4,4-difluoro-2-(8-methoxy-4-oxo-3,4-dihydrobenzofuro[3,2- dlpyrimidin-2-yl)pyrrolidine- 1 -carboxylate
[00639] (R)-tert-butyl 4,4-difluoro-2-(8-methoxy-4-oxo-3,4-dihydrobenzofuro[3,2- d]pyrimidin-2-yl)pyrrolidine-l -carboxylate was synthesized in a similar manner as to
1,1-dimethylethyl 4-(8-bromo-4-oxo-3,4-dihydrobenzofuro[3,2-d]pyrimidin-2- yl)piperidine-l -carboxylate, wherein (R)-tert-butyl 2-(2-carbamoyl-5- methoxybenzofuran-3-ylcarbamoyl)-4,4-difluoropyrrolidine-l -carboxylate replaced
1 , 1 -dimethylethyl 4-(5-bromo-2-carbamoylbenzofuran-3-ylcarbamoyl)piperidine- 1 - carboxylate. MS (EI) for C20H2IF2N3O5: 422.1 (MH+).
2-[(2S)-4,4-difluoropyrrolidin-2-yll-8-(methyloxy)[llbenzofuro[3,2-dlpyrimidin-
4(3HVone [00640] 2-[(2S)-4,4-difluoropyrrolidin-2-yl]-8-(methyloxy)[l]benzofuro[3,2- d]pyrimidin-4(3H)-one was synthesized in a similar manner to 8-bromo-2-piperidin- 4-yl[l]benzofuro[3,2-d]pyrimidin-4(3H)-one (Compound 157) wherein 1,1- dimethylethyl 4-(8-bromo-4-oxo-3,4-dihydrobenzofuro[3,2-d]pyrimidin-2- yl)piperidine-l-carboxylate was substituted with (R)-tert-butyl 4,4-difluoro-2-(8- methoxy-4-oxo-3 ,4-dihydrobenzofuro [3 ,2-d]pyrimidin-2-yl)pyrrolidine- 1 - carboxylate. 1H NMR (400 MHz, d6-DMSO): .80(d, IH), 7.46 (d, IH), 7.30 (dd, IH), 5.14 (t, IH), 4.03 (m, IH), 3.89 (m, IH), 3.13 (m, IH), 2.93 (m, IH). MS (EI) for Ci5Hi3F2N3O3: 322.2 (MH+).
(Compound 532)
2-r(2S.4R)-4-fluoropyrrolidin-2-yll-8-(methyloxy)rilbenzofuror3.2-dlpyrimidin-
4(3H)-one
(2R,4S)-tert-butyl 2-(2-carbamoyl-5-methoxybenzofuran-3-ylcarbamoyl)-4- fluoropyrrolidine- 1 -carboxylate
[00641] The intermediate (2R,4S)-tert-butyl 2-(2-carbamoyl-5- methoxybenzofuran-3-ylcarbamoyl)-4-fluoropyrrolidine- 1 -carboxylate was synthesized in a manner similar to 1,1-dimethylethyl 3-(5-bromo-2- carbamoylbenzofuran-3-ylcarbamoyl)azetidine- 1 -carboxylate 4-(tert- butoxycarbonylamino), wherein (2R,4S)- 1 -(tert-butoxycarbonyl)-4-fluoropyrrolidine- 2-carboxylic acid (commercially available from OMEGACHEM, INC.) replaced Boc- 3-azetidine carboxylic acid. The crude material from this reaction was submitted to the next step without any further purification. MS (EI) for C2OH24FN3O6: 422.2 (MH+). (2R.4S)-tert-butyl 4-fluoro-2-(8-methoxy-4-oxo-3.4-dihvdrobenzofuror3.2- d1pyrimidin-2-yl)pyrrolidine- 1 -carboxylate [00642] (2R,4S)-tert-butyl 4-fiuoro-2-(8-methoxy-4-oxo-3,4- dihydrobenzofuro[3,2-d]pyrimidin-2-yl)pyrrolidine-l -carboxylate was synthesized in a similar manner as to 1,1-dimethylethyl 4-(8-bromo-4-oxo-3,4- dihydrobenzofuro [3, 2-d]pyrimidin-2-yl)piperidine-l -carboxylate wherein (2R,4S)- tert-butyl 2-(2-carbamoyl-5 -methoxybenzofuran-3 -ylcarbamoyl)-4-fluoropyrrolidine- 1 -carboxylate was substituted for 1,1-dimethylethyl 4-(5-bromo-2- carbamoylbenzofuran-3-ylcarbamoyl)piperidine-l -carboxylate. MS (EI) for C20H22FN3O5: 404.1 (MH+). 2-r(2S.4R)-4-fiuoropyrrolidin-2-yll-8-(methyloxy)πibenzofuror3.2-dlpyrimidin- 4(3HVone
[00643] 2-[(2S,4R)-4-fiuoropyrrolidin-2-yl]-8-(methyloxy)[l]benzofuro[3,2- d]pyrimidin-4(3H)-one was synthesized in a similar manner to 8-bromo-2-piperidin- 4-yl[l]benzofuro[3,2-d]pyrimidin-4(3H)-one (Compound 157) wherein 1,1- dimethylethyl 4-(8-bromo-4-oxo-3,4-dihydrobenzofuro[3,2-d]pyrimidin-2- yl)piperidine-l-carboxylate was substituted with (2R,4S)-tert-butyl 4-fluoro-2-(8- methoxy-4-oxo-3,4-dihydrobenzofuro[3,2-d]pyrimidin-2-yl)pyrrolidine-l- carboxylate. 1H NMR (400 MHz, d6-DMSO): NMR (400 MHz, d6-DMSO): 7.80(d, IH), 7.46 (d, IH), 7.30 (dd, IH), 5.6 (d, IH), 4.95 (m, IH), 3.88 (s, 3H), 3.71 (m, 2H), 2.84 (m, IH), 2.43 (m, IH). MS (EI) for Ci5 Hi4 F N3 O3: 304.3 (MH+).
(Compound 494)
8-(methyloxy)-2-r(2^-pyrrolidin-2-ylirilbenzofuror3.2-άπpyrimidin-4(3H)-one 1 , 1 -dimethylethyl (2S)-2-( { [2-(aminocarbonyl)-5 -(methyloxy)- 1 -benzofuran-3 - yl] amino I carbonyDpyrrolidine- 1 -carboxylate
[00644] The intermediate 1,1 -dimethylethyl (25)-2-({[2-(aminocarbonyl)-5- (methyloxy)- 1 -benzofuran-3 -yl] amino } carbonyl)pyrrolidine- 1 -carboxylate_was synthesized in a manner similar to 1,1 -dimethylethyl 3-(5-bromo-2- carbamoylbenzofuran-3 -y lcarbamoyl)azetidine- 1 -carboxylate 4-(tert- butoxycarbonylamino), wherein JV-BOC-Z-proline (commercially available from Chemlmpex) replaced Boc-3-azetidine carboxylic acid. The crude material from this reaction was submitted to the next step without any further purification. MS (EI) for C20H25N3O6: 404 (MH+). l.l-dimethylethvU2S)-2-r8-('methyloxyV4-oxo-3.4-dihvdrorilbenzofuror3.2- J]pyrimidin-2-yl]pyrrolidine- 1 -carboxylate [00645] 1,1 -dimethylethyl (25)-2-[8-(methyloxy)-4-oxo-3,4- dihydro [ 1 ]benzofuro [3 ,2-<i]pyrimidin-2-yl]pyrrolidine- 1 -carboxylate was synthesized in a similar manner as to 1,1 -dimethylethyl 4-(8-bromo-4-oxo-3,4- dihydrobenzofuro [3, 2-d]pyrimidin-2-yl)piperidine-l -carboxylate wherein 1,1- dimethylethyl (2S)-2-( { [2-(aminocarbonyl)-5 -(methyloxy)- 1 -benzofuran-3 - yl] amino }carbonyl)pyrrolidine-l -carboxylate was substituted for 1,1 -dimethylethyl 4-(5-bromo-2-carbamoylbenzofuran-3-ylcarbamoyl)piperidine-l -carboxylate. 1H NMR (400 MHz, d6-DMSO): not collected. MS (EI) for C20H23N3O5 : 386 (MH+).
8-(methyloxy)-2-r(2^-pyrrolidin-2-yliri1benzofuror3.2-άπpyrimidin-4(3H)-one [00646] 8-(methyloxy)-2-[(25)-pyrrolidin-2-yl][l]benzofuro[3,2-J]pyrimidin-
4(3H)-one was synthesized in a similar manner to 8-bromo-2-piperidin-4- yl[l]benzofuro[3,2-d]pyrimidin-4(3H)-one (Compound 157) wherein 1,1- dimethylethyl 4-(8-bromo-4-oxo-3,4-dihydrobenzofuro[3,2-d]pyrimidin-2- yl)piperidine-l-carboxylate was substituted with 1,1 -dimethylethyl (25)-2-[8- (methyloxy)-4-oxo-3 ,4-dihydro [ 1 ]benzofuro [3 ,2-<i]pyrimidin-2-yl]pyrrolidine- 1 - carboxylate. 1H NMR (400 MHz, d6-DMSO): 13.45 (b s, IH), 7.79 (d, IH), 7.48 (b d, IH), 7.29 (dd, IH), 4.77 (app t, IH), 3.88 (s, 3H), 3.49 (m, 2H), 2.47 (m, IH), 2.06 (m, 3H). MS (EI) for Ci5Hi5N3O3 : 286 (MH+).
(Compound 495)
8-(methyloxy)-2-r(2i?)-pyrrolidin-2-ylirilbenzofuror3.2-άπpyrimidin-4(3H)-one 1 , 1 -dimethylethyl (2R)-2-( { r2-(aminocarbonyl)-5 -(methyloxy)- 1 -benzofuran-3 - yll amino I carbonvDpyrrolidine- 1 -carboxylate
[00647] The intermediate 1,1 -dimethylethyl (2i?)-2-({[2-(aminocarbonyl)-5- (methyloxy)- 1 -benzofuran-3 -yl] amino } carbonyl)pyrrolidine- 1 -carboxylate was synthesized in a manner similar to 1,1 -dimethylethyl 3-(5-bromo-2- carbamoylbenzofuran-3-ylcarbamoyl)azetidine- 1 -carboxylate 4-(tert- butoxycarbonylamino), wherein JV-BOC-D-proline (commercially available from
Chemlmpex) replaced Boc-3-azetidine carboxylic acid. The crude material from this reaction was submitted to the next step without any further purification. MS (EI) for C20H25N3O6: 404 (MH+). l.l-dimethylethvU2i?)-2-r8-(methyloxy)-4-oxo-3.4-dihvdrori1benzofuror3.2- (i]pyrimidin-2-yllpyrrolidine- 1 -carboxylate
[00648] 1,1 -dimethylethyl (2i?)-2-[8-(methyloxy)-4-oxo-3,4- dihydro [ 1 ]benzofuro [3 ,2-<i]pyrimidin-2-yl]pyrrolidine- 1 -carboxylate was synthesized in a similar manner as to 1,1 -dimethylethyl 4-(8-bromo-4-oxo-3,4- dihydrobenzofuro [3 ,2-d]pyrimidin-2-yl)piperidine- 1 -carboxylate wherein 1,1- dimethylethyl (2R)-2-( { [2-(aminocarbonyl)-5 -(methyloxy)- 1 -benzofuran-3 - yl] amino }carbonyl)pyrrolidine-l -carboxylate was substituted for 1,1 -dimethylethyl 4-(5-bromo-2-carbamoylbenzofuran-3-ylcarbamoyl)piperidine-l -carboxylate. 1H NMR (400 MHz, d6-DMSO): not collected. MS (EI) for C20H23N3O5 : 386 (MH+). 8-(methyloxy)-2-r(2i?)-pyrrolidin-2-ylirilbenzofuror3,2-άπpyrimidin-4(3H)-one [00649] 8-(methyloxy)-2-[(2i?)-pyrrolidin-2-yl][l]benzofuro[3,2-J]pyrimidin-
4(3H)-one was synthesized in a similar manner to 8-bromo-2-piperidin-4- yl[l]benzofuro[3,2-d]pyrimidin-4(3H)-one (Compound 157) wherein 1,1- dimethylethyl 4-(8-bromo-4-oxo-3,4-dihydrobenzofuro[3,2-d]pyrimidin-2- yl)piperidine-l-carboxylate was substituted with 1,1-dimethylethyl (2i?)-2-[8- (methyloxy)-4-oxo-3 ,4-dihydro [ 1 ]benzofuro [3 ,2-<i]pyrimidin-2-yl]pyrrolidine- 1 - carboxylate. 1H NMR (400 MHz, d6-DMSO): 13.45 (b s, IH), 7.8 (d, IH), 7.48 (b d, IH), 7.29 (dd, IH), 4.77 (b s, IH), 3.87 (s, 3H), 3.48 (m, 2H), 2.46 (m, IH), 2.09 (m, 3H). MS (EI) for Ci5Hi5N3O3 : 286 (MH+). (Compound 508) 8-bromo-2- { 1 -r(361-3-hvdroxypyrrolidin- 1 -yllethyll \ 1 lbenzofuro [3,2-JIpVrJmJdJn-
4(3H)-one
Figure imgf000293_0002
Figure imgf000293_0001
reflux EtOH, 9O0C
Figure imgf000293_0003
Figure imgf000293_0004
5 -bromo-3 -[(2-chloropropanoyl)aminol - 1 -benzofuran-2-carboxamide
[00650] A solution of 3-amino-5-bromobenzofuran-2-carboxamide 3 (1.02 g, 3.92 mmol) in 2-chloropropionyl chloride (10 mL) was heated to reflux overnight. The reaction mixture was cooled and concentrated to give a brown solid. The brown solid was triturated with ethyl acetate to afford 0.956 g of crude 5 -bromo-3 -[(2- chloropropanoyl)amino]-l -benzofuran-2-carboxamide as an off-white solid. This material was carried on without further purification. MS (EI) for Ci2HiOBrClN2O3: 345 (M+).
5-bromo-3-( {2-[(361-3-hydroxypyrrolidin- 1 -yl]propanoyl| amino)- 1 -benzofuran-2- carboxamide [00651] To a solution of 5-bromo-3-[(2-chloropropanoyl)amino]-l-benzofuran-2- carboxamide (956 mg, 2.77 mmol) in 14 mL anhydrous ethanol was added (S)-3- hydroxypyrrolidine (0.7 mL, 8.31 mmol). The reaction mixture was heated to 90 0C for 5 hours, cooled down and concentrated in vacuo. Crude 5-bromo-3-({2-[(35)-3- hydroxypyrrolidin- 1 -yljpropanoyl} amino)- 1 -benzofuran-2-carboxamide was carried on without further purification. MS (EI) for Ci6Hi8BrN3O4: 396 (M+). 8-bromo-2- { 1 -[(3y)-3-hydroxypyrrolidin- 1 -yl]ethyl| [ 1 lbenzofuro|"3,2-άπpyrimidin- 4(3HVone
[00652] To a crude solution of 5-bromo-3-({2-[(35)-3-hydroxypyrrolidin-l- yljpropanoyl} amino)- l-benzofuran-2-carboxamide (2.77 mmol) in 14 mL of ethanol was added IM aqueous NaOH (8.5mL) and heated to 120 0C overnight. The reaction mixture was brought to pH ~ 4 with IN HCl and concentrated. Purification by preparative HPLC (reverse-phase, 0.1% TFA in acetonitrile/0.05% TFA in water), followed by concentration in vacuo and lyophilization afforded 8-bromo-2-{l-[(35)- 3-hydroxypyrrolidin-l-yl]ethyl} [l]benzofuro[3,2-J]pyrimidin-4(3H)-one (372.5 mg, 35%) as a tan solid. 1U NMR (400 MHz, d6-DMSO): 8.17 (m, IH), 7.78 (m, 2H), 4.19 (b s , IH), 3.62 (m, IH), 2.84 (m, IH), 2.65 (m, 2H), 2.45 (m, IH), 1.98 (m, IH), 1.59 (m, IH), 1.41 (app dd, 3H); MS (EI) for Ci6Hi6BrN3O3: 378 (M+).
(Compound 509)
2-r(261-azetidin-2-yll-8-bromorilbenzofuror3,2-άπpyrimidin-4(3H)-one 1 , 1 -dimethylethyl (2S)-2-( { [2-(aminocarbonyl)-5 -bromo- 1 -benzofuran-3 - yl] amino I carbonyDazetidine- 1 -carboxylate
[00653] The intermediate 1,1 -dimethylethyl (25)-2-({[2-(aminocarbonyl)-5-bromo- l-benzofuran-3-yl] amino }carbonyl)azetidine-l -carboxylate was synthesized in a manner similar to 1,1 -dimethylethyl 3-(5-bromo-2-carbamoylbenzofuran-3- ylcarbamoyl)azetidine- 1 -carboxylate 4-(tert-butoxycarbonylamino), wherein N-BOC- 3-azetidine carboxylic acid (commercially available from Chemlmpex) replacedBoc- 3-azetidine carboxylic acid. The crude material from this reaction was submitted to the next step without any further purification. MS (EI) for CIgH2OBrN3Os: 438 (M+). 1,1 -dimethylethyl (25V2-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-άπpyrimidin- 2-yl)azetidine- 1 -carboxylate
[00654] l,l-dimethylethyl (25)-2-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2- <i]pyrimidin-2-yl)azetidine-l -carboxylate was synthesized in a similar manner as to 1,1 -dimethylethyl 4-(8-bromo-4-oxo-3,4-dihydrobenzofuro[3,2-d]pyrimidin-2- yl)piperidine-l -carboxylate, wherein 1,1 -dimethylethyl (2S)-2-({[2- (aminocarbonyl)-5 -bromo- 1 -benzofuran-3 -yljamino } carbonyl)azetidine- 1 - carboxylate replaced 1,1 -dimethylethyl 4-(5-bromo-2-carbamoylbenzofuran-3- ylcarbamoyl)piperidine-l -carboxylate. 1H NMR (400 MHz, d6-DMSO): not collected. MS (EI) for Ci8Hi8BrN3O4 : 420 (M+). 2-r(2y)-azetidin-2-yl1-8-bromoπibenzofuror3.2-άπpyrimidin-4(3//)-one
[00655] 2-[(25)-azetidin-2-yl]-8-bromo[l]benzoflιro[3,2-J]pyrimidin-4(3H)-one was synthesized in a similar manner to 8-bromo-2-piperidin-4-yl[l]benzofuro[3,2- d]pyrimidin-4(3H)-one (Compound 157), wherein 1,1-dimethylethyl 4-(8-bromo-4- oxo-3 ,4-dihydrobenzofuro [3 ,2-d]pyrimidin-2-yl)piperidine- 1 -carboxylate replaced 1,1-dimethylethyl (2S)-2-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin- 2-yl)azetidine-l -carboxylate. 1U NMR (400 MHz, d6-DMSO): 10.03 (b s, IH), 8.23 (b d, IH), 7.89 (m, 2H), 5.25 (app t, IH), 4.08 (app q, IH), 3.95 (app q, IH), 2.81 (app q, 2H). MS (EI) for Ci3Hi0BrN3O2 : 320 (M+). (Compound 510)
8-bromo-2-IY2,SV2.3-dihvdro- lH-indol-2-yll [ 1 lbenzofuror3.2-άπpyrimidin-4(3H)-one 1 , 1 -dimethylethyl (2S)-2-( { [2-(aminocarbonyl)-5 -bromo- 1 -benzofuran-3 - yllaminol carbonyl)-2,3-dihydro-lH-indole- 1 -carboxylate [00656] The intermediate 1,1-dimethylethyl (25)-2-({[2-(aminocarbonyl)-5-bromo- 1 -benzofuran-3 -yl] amino} carbonyl)-2,3-dihydro- lH-indole- 1 -carboxylate was synthesized in a manner similar to 1,1-dimethylethyl 3-(5-bromo-2- carbamoylbenzofuran-3-ylcarbamoyl)azetidine- 1 -carboxylate 4-(tert- butoxycarbonylamino), wherein (2S)- 1 - { [( 1 , 1 -dimethylethyl)oxy]carbonyl} -2,3- dihydro-lH-indole-2-carboxylic acid (commercially available from Chemlmpex) replaced Boc-3-azetidine carboxylic acid. The crude material from this reaction was submitted to the next step without any further purification. MS (EI) for C23H22BrN3O5: 501 (MH+).
1,1-dimethylethyl (261-2-(8-bromo-4-oxo-3,4-dihvdroπibenzofuror3,2-άπpyrimidin- 2-yl)-2,3-dihydro- lH-indole- 1 -carboxylate
[00657] 1,1-dimethylethyl (25)-2-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2- <i]pyrimidin-2-yl)-2,3-dihydro-lH-indole-l-carboxylate was synthesized in a similar manner as to 1,1-dimethylethyl 4-(8-bromo-4-oxo-3,4-dihydrobenzofuro[3,2- d]pyrimidin-2-yl)piperidine-l -carboxylate, wherein 1,1-dimethylethyl (2S)-2-({[2- (aminocarbonyl)-5 -bromo- 1 -benzofuran-3-yl]amino} carbonyl)-2,3-dihydro- IH- indole- 1 -carboxylate replaced 1 , 1 -dimethylethyl 4-(5 -bromo-2-carbamoylbenzofuran- 3-ylcarbamoyl)piperidine-l -carboxylate. 1H NMR (400 MHz, d6-DMSO): not collected. MS (EI) for C23H20BrN3O4 : 482 (M+).
8-bromo-2-IY2,SV2.3-dihvdro- lH-indol-2-yll \ 1 lbenzofuror3.2-άπpyrimidin-4(3H)-one [00658] 8-bromo-2-[(2S)-2,3-dihydro- lH-indol-2-yl] [ 1 ]benzofuro[3,2-d]pyrimidin- 4(3H)-one was synthesized in a similar manner to 8-bromo-2-piperidin-4- yl[l]benzofuro[3,2-d]pyrimidin-4(3H)-one (Compound 157) wherein 1,1- dimethylethyl 4-(8-bromo-4-oxo-3,4-dihydrobenzofuro[3,2-d]pyrimidin-2- yl)piperidine-l-carboxylate was substituted with 1,1-dimethylethyl (25)-2-(8-bromo- 4-oxo-3,4-dihydro[l]benzofuro[3,2-(i]pyrimidin-2-yl)-2,3-dihydro-lH-indole-l- carboxylate. 1H NMR (400 MHz, d6-DMSO): 8.16 (m, IH), 7.85 (m, 2H), 7.11 (m, 2H), 6.78 (m, 2H), 5.03 (app t, IH), 4.94 (b s, IH), 3.45 (m, 2H). MS (EI) for C18H12BrN3O2 : 382 (M+). (Compound 523)
8-bromo-2-[(261-5-oxopyrrolidin-2-yll[llbenzofuro[3,2-(i]pyrimidin-4(3H)-one 1 , 1 -dimethylethyl (2S)-2-( { r2-(aminocarbonyl)-5 -bromo- 1 -benzofuran-3 - yll amino I carbonvD-5 -oxopyrrolidine- 1 -carboxylate [00659] The intermediate 1,1-dimethylethyl (25)-2-({[2-(aminocarbonyl)-5-bromo- 1 -benzofuran-3 -yl] amino } carbonyl)-5 -oxopyrrolidine- 1 -carboxylate was synthesized in a manner similar to 1,1-dimethylethyl 3-(5-bromo-2-carbamoylbenzofuran-3- ylcarbamoyl)azetidine-l -carboxylate 4-(tert-butoxycarbonylamino), wherein 1-{[(1,1- dimethylethyl)oxy]carbonyl}-5-oxo-L-proline (commercially available from Chemlmpex) replaced Boc-3-azetidine carboxylic acid. The crude material from this reaction was submitted to the next step without any further purification. MS (EI) for Ci9H20BrN3O6: 466 (M+). Λ/-[2-(aminocarbonyl)-5-bromo-l-benzofuran-3-yll-5-oxo-L-prolinamide
[00660] JV-[2-(aminocarbonyl)-5 -bromo- 1 -benzofuran-3-yl]-5-oxo-L-prolinamide was synthesized in a similar manner to 8-bromo-2-piperidin-4-yl[l]benzofuro[3,2- d]pyrimidin-4(3H)-one (Compound 157), wherein 1,1-dimethylethyl 4-(8-bromo-4- oxo-3 ,4-dihydrobenzofuro [3 ,2-d]pyrimidin-2-yl)piperidine- 1 -carboxylate was substituted with 1,1-dimethylethyl (25)-2-({[2-(aminocarbonyl)-5-bromo-l- benzofuran-3-yl]amino}carbonyl)-5-oxopyrrolidine-l -carboxylate. 1H NMR (400 MHz, de-DMSO): not collected. MS (EI) for Ci4Hi2BrN3O4 : 366 (M+). 8-bromo-2-r(261-5-oxopyrrolidin-2-yliπibenzofuror3,2-άπpyrimidin-4(3H)-one [00661] 8-bromo-2-[(25)-5-oxopyrrolidin-2-yl][l]benzofuro[3,2-(i]pyrimidin- 4(3H)-one was synthesized in a similar manner as to 1,1-dimethylethyl 4-(8-bromo-4- oxo-3 ,4-dihydrobenzofuro [3 ,2-d]pyrimidin-2-yl)piperidine- 1 -carboxylate, wherein Λ/-[2-(aminocarbonyl)-5-bromo- 1 -benzofuran-3-yl]-5-oxo-L-prolinamide replaced 1 , 1 -dimethylethyl 4-(5-bromo-2-carbamoylbenzofuran-3-ylcarbamoyl)piperidine- 1 - carboxylate. 1U NMR (400 MHz, d6-DMSO): not collected. MS (EI) for Ci4Hi0Br N3O3 : 348 (M+). EXAMPLE 29
Figure imgf000297_0001
(Compound 286)
8-bromo-2-[(4-hydroxy-2-oxopyrrolidin- 1 -yPmethyl] [ 1 "|benzofuro|"3 ,2-d]pyrimidin- 4(3H)-one
[00662] To a solution of 4-(trimethylsilyloxy)pyrrolidin-2-one (166 mg, 1.6 mmol, reference: synthesis, 1978, 614-617) in DMA (5mL) was added lithium tert-butoxide (128 mg, 1.6 mmol) at rt. The recation mixture was stirred at rt for 20 min, 8-bromo- 2-(chloromethyl)benzofuro[3,2-d]pyrimidin-4(3H)-one 6 (200 mg, 0.64 mmol) was added to the solution. The mixture was stirred for 3 hours. 1 N aquous HCl solution (5 mL) was added, and the reaction was stirred 2 hours at rt and concentrated. Purification by preparative HPLC afforded 8-bromo-2-[(4-hydroxy-2-oxopyrrolidin- l-yl)methyl][l]benzofuro[3,2-d]pyrimidin-4(3H)-one (60 mg). 1H NMR (400 MHz, de-DMSO): 8.20 (s, IH), 7.82 (s, 2H), 4.61 (d, IH), 4.45-4.37 (m, 2H), 3.86-3.76 (m, IH), 3.35 (dd, IH), 2.70 (dd, IH), 2.19 (dd, IH); MS (EI) for Ci5Hi2BrN3O4: 379 (MH+).
(Compound 287)
8-bromo-2- { [3-(hydroxymethyl)pyrrolidin- 1 -yl]methyl| [ 1 lbenzofuro[3 ,2- dlpyrimidin-4(3H)-one [00663] To a solution of 3-hydroxymethyl-pyrroldine-l-carboxylic acid tert-butyl ester (201 mg, 1 mmol) in EtOAc (5 mL) was added 4 N HCl in dioxane (2 mL). The reaction mixture was stirred at room temp overnight and concentrated in vacuo. The residue was dissolved in ethanol (5 mL), followed by 8-bromo-2- (chloromethyl)benzofuro[3,2-d]pyrimidin-4(3H)-one 6 (1 mmol) and triethylamine (1 ml). The mixture was heated at 50 0C for 5 hours and concentrated. The residue was purified by preparative HPLC to afford 8-bromo-2-{[3-(hydroxymethyl)pyrrolidin-l- yl]methyl}[l]benzofuro[3,2-d]pyrimidin-4(3H)-one (142 mg). 1U NMR (400 MHz, d6-DMSO): 8.22 (s, IH), 7.82 (s, 2H), 3.90 (s, 2H), 3.40-3.30 (m, 3H), 3.00-2.80 (m, 2H), 2.65-2.60 (m, IH), 2.40-2.30 (m, IH), 1.95-1.85 (m, IH), 1.55-1.46 (m, IH); MS (EI) for Ci7Hi6BrN3O3: 390 (MH+); MS (EI) for Ci6Hi6BrN3O3: 378 (MH+).
(Compound 288)
8-bromo-2-{[(pyrrolidin-3-ylmethyl)oxylmethyU[llbenzofuro[3,2-dlpyrimidin- 4(3HVone [00664] To a solution of S-hydroxymethyl-pyrroldine-l-carboxylic acid tert-butyl ester (201 mg, 1 mmol) in DMF (5 mL) was added NaH (50 mg, 60% in oil). After stirring at rt for 10 min, 8-bromo-2-(chloromethyl)benzofuro[3,2-d]pyrimidin-4(3H)- one 6 (155 mg, 0.5 mmol) was added in one portion. The reaction mixture was stirred at rt for 1 hour. 4 N HCl in dioxane (2 mL) was added, the reaction was heated to 50 0C for 2 hours. The reaction mixture was filtered, the filtrate was concentrated. Purification of the residue by preparative HPLC afforded 128 mg of 8-bromo-2- {[(pyrrolidin-3-ylmethyl)oxy]methyl} [l]benzofuro[3,2-d]pyrimidin-4(3H)-one. ). 1H NMR (400 MHz, d6-DMSO): 8.37 (s, IH), 8.17 (s, IH), 7.79 (s, 2H), 4.45 (dd, 2H), 3.58 (d, 2H), 3.27-3.18 (m, 2H), 3.16-3.09 (m, 2H), 2.62-2.58 (m, IH), 2.09-2.95 (m, IH), 1.78-1.69 (m, IH); MS (EI) for CnHi6BrN3O3: 390 (MH+); MS (EI) for Ci6Hi6BrN3O3: 378 (MH+). EXAMPLE 30
(Compound 346)
8-bromo-2-|Y3 -hydroxy-3 -methylpyrrolidin- 1 -yDmethyl] [ 1 ]benzofuro [3,2- d1pyrimidin-4(3H)-one
Figure imgf000298_0001
Preparation of 3-methylpyrrolidin-3-ol
[00665] To a solution of JV-tert-butoxycarbonyl-3-pyrrolidinone (1.0 g, 5.4 mmol) in THF (10 mL) was added methylmagnesium bromide (3 M in ether, 6 mL) dropwise at 0 0C. The reaction mixture was allowed to warm up to rt and stirred another 2 hours. The reaction was quenched with water (2 mL). The reaction mixture was partitioned between EtOAc and water. The organic layer was washed with brine, dried and concentrated. The residue was dissoved in TFA (3 mL), stirred at rt for 3 hours and concentrated to a black solid, which was used to the preparation of 8-bromo-2- [(3 -hydroxy-3 -methylpyrrolidin- 1 -yl)methyl] [ 1 Jbenzofuro [3 ,2-d]pyrimidin-4(3H)-one without further purification.
[00666] To a solution of 8-bromo-2-(chloromethyl)[l]benzofuro[3,2-d]pyrimidin- 4(3H)-one 6 (100 mg, 0.32 mmol) in 5 mL anhydrous ethanol was added crude 3- hydroxypyrrolidine-3-carboxylic acid (500 mg, excess, prepared above) and triethylamine (2 mL). The reaction mixture was heated to 80 0C overnight. The reaction was cooled and concentrated. The purification of the residue by preparative HPLC gave the title compound as a white solid. 1H NMR (400 MHz, d6-DMSO): 8.21 (m, IH), 7.82 (m, 2H), 3.78 (dd, 2H), 2.92 (dd, IH), 2.65 (d, IH), 2.55 (m, 2H), 1.79 (t, IH), 1.24 (s, 3H); MS (EI) for Ci6Hi4BrN3O3: 378 (MH+). Preparation of cis-3 ,4-dihydroxypyrrolidine :
"N"
H
Figure imgf000299_0001
cis-3 ,4-dihydroxypyrrolidine
[00667] To a solution of benzyl chloroformate (3.4 g, 20 mmol) in DCM (10 mL) was added 3-pyrroline (1.46 g) dropwise at ) 0 0C. After stirring overnight at rt, the reaction mixture was washed with 0.5 N HCl and saturate aq. NaHCO3 solution. The organic layer was dried and concentrated to give l-(benzyloxycarbarbonyl)-3- pyrroline (2.5 g) as a white solid. 1H NMR (400 MHz, CDCl3): 7.42-7.28 (m, 5H), 5.80 (m, 2H), 5.17 (s, 2H), 4.20 (m, 4H). [00668] l-(benzyloxycarbarbonyl)-3-pyrroline (1.02 g, 5mmol) was dissolved in THF (20 mL) and treated with OsO4 (2 mL, 4% in water) followed by N- methylmorpholine N-oxide (585 mg, 5 mmol). After 5 hours, LC-MS indicated the reaction is complete. The solvent was evoporated, the residue was dissolved in EtOAc and washed with diluted Na2SO3 solution, saturated NaHCO3, brine and dried. Concentration and purification by silica gel chromatography gave 630 mg of 1- (benzyloxycarbarbonyl)-cis-3,4-dihydroxypyrrolidine. 1H NMR (400 MHz, CDCI3): 7.40-7.28 (m, 5H), 5.16 (s, 2H), 4.27 (m, 2H), 3.65 (m, 2H), 3.42 (m, 2H), 2.63 (m, 2H). [00669] A round-bottomed flask was charged with l-(benzyloxycarbarbonyl)-cis- 3,4-dihydroxypyrrolidine (600 mg), ethanol (30 mL), Pd/C (10% in weight, wet, 100 mg) and hydrogen balon. The reaction was stirred at rt overnight. LC-MS indicated the starting material was dissappeared. The reaction mixture was filtered, concentrated. The residue was used to next step without further purification. 1H NMR (400 MHz, CDCl3): 4.21 (m, 2H), 3.60 (m, 2H), 3.21 (m, 2H), 2.80 (m, 2H).
(Compound 407)
8-bromo-2-[(3-ethyl-3-hydroxypyrrolidin-l-yl)methyll[llbenzofuro[3,2-dlpyrimidin- 4(3HVone
[00670] 8-bromo-2-[(3-ethyl-3-hydroxypyrrolidin-l-yl)methyl][l]benzofuro[3,2- d]pyrimidin-4(3H)-one was synthesized in a manner similar to Compound 346, wherein methylmagnesium bromide was substituted with ethylmagnesium bromide. The purification of the residue by preparative HPLC gave the title compound as a white solid. 1H NMR (400 MHz, CDOD3): 8.21 (s, IH), 7.78 (d, IH), 7.75 (d, IH), 4.06 (dd, 2H), 3.35 (m, IH), 3.05-2.95 (m, 2H), 2.90 (d, IH), 2.08-1.91 (m 2H), 1.70 (q, 2H), 0.95 (t, 3H); MS (EI) for Ci7Hi8BrN3O3: 392 (MH+).
EXAMPLE 31
Figure imgf000301_0001
[00671] Ethyl 6-bromoimidazo[l,2-a]pyridine-2-carboxylate, 2, was synthesized according to the reference: J. Org. Chem. 1965, 2403-2407.
[00672] Ethyl 6-bromo-3-nitroimidazo[l,2-a]pyridine-2-carboxylate, 3,was synthesized according to the reference: J. Org. Chem. 1981, 46, 1026-1030. 1H NMR (400 MHz, CDCl3): 9.55 (s, IH), 7.80 (s, IH), 7.25 (s, IH), 4.58 (q, 2H), 1.45 (t, 3H). 6-bromo-3-nitroimidazoπ ,2-alpyridine-2-carboxamide 4 [00673] To a solution of ethyl 6-bromo-3-nitroimidazo[l,2-a]pyridine-2- carboxylate, 3, (3.13 g, 10 mmol) in THF (40 rnL) was added concentrated ammonium hydroxide (100 mL). The reaction was stirred at rt for 4 days. The suspension was filtered to give 6-bromo-3-nitroimidazo[l,2-a]pyridine-2- carboxamide (2.2 g, 78% yield) as a pale yellow solid. 1U NMR (400 MHz, CDCl3): 9.40 (s, IH), 8.18 (s, IH), 8.03 (m, 3H).
3-amino-6-bromoimidazor 1 ,2-alpyridine-2-carboxamide 5
[00674] To hydrobronic acid (24 mL) cooled to -10 0C was added tin (3.23 g, 26.2 mmol). 6-bromo-3-nitroimidazo[l,2-a]pyridine-2-carboxamide (3.1 g, 10.8 g) was added partionwise to avoid a temperature of more than 5 0C. The mixture was stirred at 0 0C for 1 hour, allowed to stand at rt and stirred another 1 hour. The suspension was filtered, the filtrate was made basic with half saturated sodium carbonate. The suspension was stirred for a few min., then filtered to give 3-amino-6- bromoimidazo[l,2-a]pyridine-2-carboxamide as pale yellow solid (6.0 g). 1H NMR (400 MHz, d6-DMSO): 8.90 (s, IH), 7.80 (m, 2H)
(Compound 419)
8-bromo-2-(chloromethyl)pyridor 1 ,2-e1purin-4(3H)-one [00675] To a solution of 3 -amino-6-bromoimidazo[l,2-a]pyridine-2-carboxamide (1.0 g) in DMA (10 niL) was added 2-chloroacetyl chloride (20 mL) at rt. The reaction mixture was heated to 40 0C for 5 hours. LC-MS indicated that the reaction is complete. The reaction was concentrated to remove 2-chloroacetyl chloride in vacuo. To the remaining DMA solution was added 1 N NaOH (20 mL). After stirring at 40 0C for 1 hours, the reaction was complete. The reaction was cooled and neutralized with 3 N HCl to PH 5-6, then extracted with EtOAc. The organic layer was separated and dried. The residue was used to next stpe without purification. 1H NMR (400 MHz, d6-DMSO): 10.63 (s, IH), 87.42 (s, IH), 7.75 (s, IH), 7.60 (m, IH), 7.50 (m, 2H), 4.45 (s, 2H). (Compound 417)
8-bromo-2-[(4-methylpiperazin-l-yl)methyllpyrido[l,2-elpurin-4(3H)-one
[00676] To a solution of 8-bromo-2-(chloromethyl)pyrido[l,2-e]purin-4(3H)-one (Compound 419) (100 mg, O.lOmmol) in 3 mL anhydrous ethanol was added 1- methylpiperazine (3 eq.). The reaction mixture was heated to 80 0C for 2 hours, cooled down and concentrated in vacuo. Purification by preparative HPLC resulting in 8-bromo-2-[(4-methylpiperazin-l-yl)methyl]pyrido[l,2-e]purin-4(3H)-one as a solid. 1H NMR (400 MHz, d6-DMSO): 8.85 (s, IH), 8.27 (s, 2H), 7.70 (d, IH), 7.60 (d, IH), 3.60 (m, 4H), 2.35 (m, 4H), 2.17 (s, 3H); MS (EI) for Ci5Hi7BrN6O: 377 (MH+). (Compound 418)
8-bromo-2-{r(3S)-3-hvdroxypyrrolidin-l-yllmethvUpyridori,2-elpurin-4(3H)-one
[00677] 8-bromo-2-{[(3S)-3-hydroxypyrrolidin-l-yl]methyl}pyrido[l,2-e]purin- 4(3H)-one was synthesized in a manner similar to Compound 417, wherein 1- methylpiperazine was substituted with S-(-)3-hydroxypyrrolidine. Preparative HPLC purification gave the title compound as a white solid. 1H NMR (400MHz, dβ-DMSO): 8.82 (s, IH), 8.25 (s, IH), 7.68 (d, IH), 7.59 (d, IH), 4.19 (s, 2H), 3.70 (m, IH), 2.78 (m, 2H), 2.00 (m, 2 H), 1.80 (m, 2H); MS (EI) for Ci4Hi4BrN5O2: 364 (MH+). (Compound 416)
8-bromo-2-{2-[(3-methylbutyl)oxylphenyl|pyrido[l,2-elpurin-4(3H)-one
[00678] A solution of 3-amino-6-bromoimidazo[ 1 ,2-a]pyridine-2-carboxamide (200 mg, 0.75 mmol) and 2-(isopentyloxy)benzaldehyde (200 mg) in 6 niL anhydrous ethanol and 10 mL DMA was heated at 80 0C overnight. Then sodium bisulfite (300) and DMSO (3 mL) was added and the reaction was heated at 150 0C for 2 days. After cooling, the reaction mixture was filtered and the filtrate was purified by preparative HPLC to give the title compound as a white solid. 1H NMR (400 MHz, d6-DMSO): 12.20 (s, IH), 8.95 (s, IH), 7.98 (d, IH), 7.68 (d, IH), 7.60 (dd, IH), 7.57 (m, IH), 7.23 (d, IH), 7.10 (t, IH), 4.15 (t, 2H), 1.80-1.65 (m, 3H), 0.90 (s, 6H); MS (EI) for C20Hi9BrClN4O2: 327 (MH+).
(Compound 450)
8-(3 -hydroxyprop- 1 -vn- 1 -yl)-2- [(4-methylpiperazin- 1 -vDmethyll [ 1 lbenzofuro [3 ,2- dlpyrimidin-4(3H)-one [00679] 8-(3-hydroxyprop-l-yn-l-yl)-2-[(4-methylpiperazin-l- yl)methyl][l]benzofuro[3,2-d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 31 wherein 8-bromo-2-[(4-methylpiperazin-l- yl)methyl][l]benzofuro[3,2-d]pyrimidin-4(3H)-one Compound 3 was substituted with 8-bromo-2- { [(3 S)-3 -hydroxypyrrolidin- 1 -yl]methyl} [ 1 ]benzofuro [3 ,2- d]pyrimidin-4(3H)-one . 1H NMR (400 MHz, d6-DMSO): 7.98 (s, IH), 7.79 (m, IH), 7.64 (m, IH), 4.32 (s, 2H), 3.48 (s, 2H), 2.31 (m, 4H), 2.12 (s, 3H), 1.82 (m, 6H). MS (EI) for C19 H20 N4 03: 353 (MH+).
(Compound 456)
8-(6-hydroxyhex- 1 -vn- 1 -yl)-2- { [(3 S)-3 -hydroxypyrrolidin- 1 - vHmethvUrHbenzofuror3,2-dlpyrimidin-4(3H)-one
[00680] 8-(6-hydroxyhex- 1 -yn- 1 -yl)-2- {[(3 S)-3 -hydroxypyrrolidin- 1 - yl]methyl}[l]benzofuro[3,2-d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 31 wherein hex-5-yn-l-ol was substituted with propargyl alcohol.
1H NMR (400 MHz, d6-DMSO): 7.96 (m, IH), 7.79 (m, IH), 7.62 (m, IH), 4.21 (s, IH), 3.68 (m, 2H), 3.46 (m, 2H), 2.80 (m, 2H), 2.54 (m, 2H), 2.47 (m, 2H), 2.01 (m,
IH), 1.61 (m, 5H). MS (EI) for C21 H23 N3 04: 382 (MH+). (Compound 458)
8-ethynyl-2- { [(3 S)-3 -hydroxypyrrolidin- 1 -yl"|methyl| [ 1 Ibenzofuro [3 ,2-d]pyrimidin- 4(3HVone
[00681] 8-ethynyl-2-{[(3S)-3-hydroxypyrrolidin-l-yl]methyl}[l]benzofuro[3,2- d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 31 wherein trimethylsilylacetylene was substituted with propargyl alcohol. 1H NMR (400 MHz, d6-DMSO): 8.08 (s, IH), 7.82 (d, IH), 7.71 (d, IH), 4.26 (s, IH), 4.19 (s, IH), 3.67 (m, 2H), 2.80 (m, 2H), 2.53 (m, 2H), 2.01 (m, IH), 1.59 (m, IH). MS (EI) for C17 H15 N3 O3: 310 (MH+). (Compound 459)
2- ( IY3 SV 3-hvdroxypyrrolidin- 1 -yllmethyll -8- [(trimethylsilvDethynvH [ 1 "IbenzofuroB ,2-dlpyrimidin-4(3H)-one [00682] 2-{[(3S)-3-hydroxypyrrolidin-l-yl]methyl}-8-
[(trimethylsilyl)ethynyl][l]benzofuro[3,2-d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 31 wherein trimethylsilylacetylene was substituted with propargyl alcohol. 1H NMR (400 MHz, d6-DMSO): 7.78 (s, IH), 7.56 (d, IH), 7.44 (d, IH), 3.93 (s, IH), 3.41 (s, 2H), 2.54 (m, 2H), 2.28 (m, 2H), 1.75 (m, IH), 1.33 (m, IH), 0.00 (s, 9H). MS (EI) for C20 H23 N3 03 Si: 383 (MH+).
Figure imgf000304_0001
(Compound 454)
8-(3 -hydroxyprop- 1 -yn- 1 -yl)-2- { [(3 S)- 3 -hydroxypyrrolidin- 1 - vHmethvUrHbenzofuror3,2-dlpyrimidin-4(3H)-one
[00683] To a solution of 8-bromo-2-{[(3S)-3-hydroxypyrrolidin-l- yl]methyl}[l]benzofuro[3,2-d]pyrimidin-4(3H)-one (50 mg, 0.14 mmol) in tert- butanol (1.5 mL) and water (0.3 mL) was added CS2CO3 (67 mg, 0.21 mmol), propargyl alcohol (40 μL, 0.69 mmol), [l,l '-bis(di-tert-butylphosphino)ferrocene] Palladium dichloride (17.9 mg, 0.03 mmol), and Cu(OTf) toluene complex (71 mg, 0.14 mmol). The reaction mixture was heated at 120 0C in the microwave for 10 minutes. Upon cooling, the reaction mixture was filtered through a PL-Thiol MP SPE cartridge and rinsed with 3 mL MeOH. The filtrate was concentrated in vacuo and the residue re-suspended in 1.5 mL MeOH. This suspension was filtered through a 0.2 micron syringe filter and purified by preparatory HPLC (reverse phase, acetonitrile/water with 0.1% NH4OACZACOH), followed by concentration in vacuo and lyophilization to afford 23 mg (24%) of the title compound. 1H NMR (400 MHz, d6-DMSO): 7.99 (s, IH), 7.81 (m, IH), 7.66 (m, IH), 4.34 (s, 2H), 4.20 (s, IH), 3.68 (s, 2H), 2.80 (m, 2H), 2.55 (m, 2H), 2.01 (m, IH), 1.60 (m, IH). MS (EI) for C18 H17 N3 04: 340 (MH+). Example 32
Figure imgf000305_0001
N-(5 -bromo-2-carbamoylbenzofuran-3 -vD-3 -chloroisonicotinamide [00684] To a solution of 3-Chloro-4-pyridinecarboxylic acid (594 mg, 3.77 mmol) in dichloromethane (8 mL), dimethylacetamide (2 mL) and diisopropylethylamine (1168 μL, 7.07 mmol) was added HATU (1433 mg, 3.77 mmol) followed by 3- amino-5-bromobenzofuran-2-carboxamide (400 mg, 1.57 mmol). The reaction was heated to 50 0C overnight. The reaction mixture was cooled to room temperature. The precipitate was filtered, rinsed with ethyl acetate and dried under vacuum to give 460 mg (74%) of N-(5-bromo-2-carbamoylbenzofuran-3-yl)-3-chloroisonicotinamide. IH NMR (400 MHz, d6-DMSO): 10.93 (s, IH), 8.83 (s, IH), 8.73 (d, IH), 8.23 (s, br, IH), 8.16 (s, IH), 7.96 (s, br, IH), 7.80 (d, IH), 7.67 (m, 2H). MS (EI) for C15 H9 Br Cl N3 03: 396 (MH+).
(Compound 112)
8-bromo-2-(3-chloropyridin-4-yl)rilbenzofuror3,2-dlpyrimidin-4(3H)-one [00685] A suspension of N-(5-bromo-2-carbamoylbenzofuran-3-yl)-3- chloroisonicotinamide (260 mg, 0.66 mmol) and 2.6 M aqueous NaOH (609 μL, 1.58 mmol) in 3 mL anhydrous ethanol was heated for 20 min at 150 0C in a microwave reactor. The reaction mixture was diluted with 12 mL of water and acidified with IM HCl. Precipitate was filtered, rinsed with ethanol, and stirred in 15 mL of ethanol at 50 0C for 30 minutes. It was then filtered, rinsed with ethanol, and dried under vacuum to give 190 mg (73%) of 8-bromo-2-(3-chloropyridin-4-yl)[l]benzofuro[3,2- d]pyrimidin-4(3H)-one. IH NMR (400 MHz, d6-DMSO): 13.60 (s, IH), 8.88 (s, IH), 8.74 (d, IH), 8.26 (s, IH), 7.88 (m, 2H), 7.77 (d, IH). MS (EI) for C15 H7 Br C1 N3 02: 378 (MH+).
(Compound 460)
8-ethyl-2- { [(3 S)- 3 -hydroxypyrrolidin- 1 -yllmethyl) \ 1 lbenzofuro [3 ,2-dlpyrimidin- 4(3H)-one [00686] 8-ethyl-2- { [(3 S)-3 -hydroxypyrrolidin- 1 -yl]methyl} [ 1 ]benzofuro [3 ,2- d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 32 wherein trimethylsilylacetylene was substituted with propargyl alcohol. 1H NMR (400 MHz, d6-cdCl3): 7.88 (s, IH), 7.61 (d, IH), 7.44 (d, IH), 4.45 (t, IH), 4.00 (d, IH), 3.85 (d, IH), 3.73 (m, IH), 3.18 (m, IH), 3.03 (d, IH), 2.77 (m, 3H), 2.45 (m, IH), 2.33 (m, IH), 1.99 (m, IH), 1.32 (t, 3H), 1.25 (t, IH). MS (EI) for C17 H19 N3 03: 314 (MH+).
(Compound 492)
2-r(lS)-l-aminoethyll-8-bromoπibenzofuror3,2-dlpyrimidin-4(3H)-one [00687] 2-[(lS)-l-aminoethyl]-8-bromo[l]benzofuro[3,2-d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 32 wherein Boc-L-alanine was substituted with 2-chloro-4(methylsulfonyl)benzoic acid. 1H NMR (400 MHz, d6-
DMSO): 8.05 (s, IH), 7.77 (m, 2H), 4.07 (m, IH), 1.46 (d, 3H). MS (EI) for C12 HlO Br N3 02: 309 (MH+).
(Compound 457)
8-(6-hydroxyhexyl)-2- { [(3 S)- 3 -hydroxypyrrolidin- 1 -yl]methyl| [ 1 lbenzofuro [3,2- dlpyrimidin-4(3H)-one
[00688] 8-(6-hydroxyhexyl)-2- { [(3 S)-3 -hydroxypyrrolidin- 1 - yl]methyl}[l]benzofuro[3,2-d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 32 wherein hex-5-yn-l-ol_ was substituted with propargyl alcohol. 1H NMR (400 MHz, d6-DMSO): 7.81 (m, IH), 7.69 (m, IH), 7.49 (m, IH), 4.19 (s, IH), 3.66 (m, 2H), 3.35 (m, 4H), 2.75 (m, 4H), 2.00 (s, IH), 1.62 (m, 3H), 1.39 (m, 2H), 1.30 (m, 4H). MS (EI) for C21 H27 N3 04: 386 (MH+).
Figure imgf000307_0001
(Compound 455)
8-(3-hydroxypropyl)-2-{[(3S)-3-hydroxypyrrolidin-l-yllmethyl|[llbenzofuro[3,2- d]pyrimidin-4(3H)-one [00689] To a solution of 8-bromo-2-{[(3S)-3-hydroxypyrrolidin-l- yl]methyl}[l]benzofuro[3,2-d]pyrimidin-4(3H)-one (50 mg, 0.14 mmol) in tert- butanol (1.5 niL) and water (0.3 mL) was added CS2CO3 (67 mg, 0.21 mmol), propargyl alcohol (40 μL, 0.69 mmol), [l,r-bis(di-tert-butylphosphino)ferrocene] Palladium dichloride (17.9 mg, 0.03 mmol), and Cu(OTf) toluene complex (71 mg, 0.14 mmol). The reaction mixture was heated at 120 0C in the microwave for 10 minutes. Upon cooling, the reaction mixture was transferred to a hydrogenation vessel with the addition of a scope of Pd/C. The reaction was connected to pan- shaker and shook overnight. The reaction mixture was filtered through a PL-Thiol MP SPE cartridge and rinsed with 3 mL MeOH. The filtrate was concentrated in vacuo and the residue re-suspended in 1.5 mL MeOH. This suspension was filtered through a 0.2 micron syringe filter and purified by preparatory HPLC (reverse phase, acetonitrile/water with 0.1% NH4OACZACOH), followed by concentration in vacuo and lyophilization to afford 13 mg (14%) of the title compound. 1H NMR (400 MHz, d6-DMSO): 7.82 (m, IH), 7.70 (m, IH), 7.49 (m, IH), 4.19 (s, IH), 3.66 (m, 2H), 3.43 (m, 2H), 2.78 (m, 4H), 2.20 (s, IH), 1.84 (m, 2H), 1.77 (m, 2H), 1.58 (s, IH). MS (EI) for C18 H21 N3 04: 344 (MH+). Example 33
Figure imgf000307_0002
(Compound 284)
2-amino-N-[4-(8-bromo-4-oxo-3,4-dihydro[llbenzofuro[3,2-dlpyrimidin-2-yl)-3- chlorophenyllpyridine-4-carboxamide
[00690] To a solution of 2-acetamidoisonicotinic acid (368 mg, 2.04 mmol) in dimethylacetamide (5 niL) and diisopropylethylamine (675 μL, 4.08 mmol) was added 2-(4-amino-2-chlorophenyl)-8-bromo[l]benzofuro[3,2-d]pyrimidin-4(3H)-one (200 mg, 0.51 mmol) followed by HATU (776 mg, 2.04 mmol). The reaction mixture was heated at 50 0C overnight. The reaction mixture was cooled to room temperature, diluted with 50 mL of ice water. The precipitate was filtered and dried under vacuum to give 180 mg (64%) of 2-acetamido-N-(4-(8-bromo-4-oxo-3,4- dihydrobenzofuro[3,2-d]pyrimidin-2-yl)-3-chlorophenyl)isonicotinamide. The resulting product was used without further purification. A suspension of 2-acetamido- N-(4-(8-bromo-4-oxo-3,4-dihydrobenzofuro[3,2-d]pyrimidin-2-yl)-3- chlorophenyl)isonicotinamide (180 mg, 0.32 mmol) and concentrated HCl (200 μL) in 4 mL anhydrous ethanol was heated for 20 minutes at 120 0C in a microwave reactor. The reaction mixture was neutralized with diisopropylethylamine and purified by preparative HPLC (reverse-phase, acetonitrile/water with 0.01% formic acid) to give 40 mg (15%) of 2-amino-N-[4-(8-bromo-4-oxo-3,4- dihydro [ 1 ]benzofuro [3 ,2-d]pyrimidin-2-yl)-3 -chlorophenyl]pyridine-4-carboxamide . IH NMR (400 MHz, d6-DMSO): 10.67 (s, IH), 8.23 (d, 2H), 8.09 (m, 2H), 7.84 (m, 3H), 7.67 (d, IH), 6.96 (d, IH), 6.90 (s, IH), 6.29 (s, 2H). MS (EI) for C22 H13 Br Cl N5 03: 512 (MH+).
EXAMPLE 34
Figure imgf000308_0001
(Compound 93)
8-Bromo-2-(3-chlorophenyl)πibenzofuror3,2-dlpyrimidin-4(3H)-one [00691] A solution of 3-amino-5-bromobenzofuran-2-carboxamide 3 (0.5Og , 0.1.96 mmol) and 3-chlorobenzaldehyde (607 μL, 3.92 mmol) in 6 mL anhydrous ethanol were combined and stirred at room temperature for 10 minutes. The resulting suspension was treated with concentrated hydrochloric acid (40 μL) and a precipitate formed immediately. The resulting slurry was diluted with additional anhydrous ethanol (10 ml) and the resulting slurry was heated at 80 0C for 16 hours. The resulting precipitate was filtered off and washed with ethyl acetate (2 x 50 ml) and methanol (2 x 5 ml) to give 76 mg of 8-bromo-2-(3-chlorophenyl)[l]benzofuro[3,2- d]pyrimidin-4(3H)-one. 1H NMR (400 MHz, d6-DMSO): 13.38 (br s, IH), 8.23 (d, IH), 7.91 (dd, IH), 7.85 (s, IH), 7.84 (d, IH), 7.71 (dd, IH), 7.52 (dd, IH), 7.46 (d, IH). MS (EI) for Ci6 H8 Br Cl N2 O2: 377 (MH+). (Compound 94)
8-Bromo-2-(4-chlorophenyl)πibenzofuror3,2-dlpyrimidin-4(3H)-one [00692] 8-Bromo-2-(4-chlorophenyl)[l]benzofuro[3,2-d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 34 wherein 4-chlorobenzaldehyde was substituted with 3-chlorobenzaldehyde. 1H NMR (400 MHz, d6-DMSO): 13.27 (br s, IH), 8.27 (s, IH), 8.11 (d, 2H), 7.86 (d, 2H), 7.80 (s, IH), 7.78 (s, IH). MS (EI) for C16 H8 Br Cl N2 O2: 377 (MH+).
(Compound 95)
8-Bromo-2-(4-bromophenyl)[llbenzofuro[3,2-dlpyrimidin-4(3H)-one [00693] 8-Bromo-2-(4-bromophenyl)[l]benzofuro[3,2-d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 34 wherein 4-bromobenzaldehyde was substituted with 3-chlorobenzaldehyde. 1H NMR (400 MHz, d6-DMSO): 13.27 (br s, IH), 8.27 (s, IH), 8.23 (d, 2H), 7.92 (d, 2H), 7.80 (s, IH), 7.78 (s, IH). MS (EI) for Ci6 H8 Br2 N2 O2: 421 (MH+).
EXAMPLE 35 (Compound 96)
10-(2-Chlorophenvnnaphthorr.2':4.51furor3.2-dlpyrimidin-8(9H)-one
Figure imgf000310_0001
2-Hydroxy- 1 -naphthonitrile [00694] 2-Hydroxy- 1 -naphthonitrile was synthesized in a manner similar to 2- hydroxy-5-methoxybenzonitrile (Example 10) wherein 2-hydroxynaphthalene-l- carboxaldehyde was substituted with 2-hydroxy-5-methoxybenzaldehyde. 1H NMR
(400 MHz, de-DMSO): 11.40 (br s, IH), 8.10 (d, lH)m 7.95 (d, IH), 7.88 (d, IH),
7.68 (t, IH), 7.46 (t, IH), 7.20 (d, IH). MS (EI) for Cn H7NO: 170 (MH+).
2-(l-Cyanonaphthalen-2-yloxy)acetamide
[00695] 2-(l-Cyanonaphthalen-2-yloxy)acetamide was synthesized in a manner similar to 2-(4-bromo-2-cyanophenoxy)acetamide 2, wherein 2-hydroxy-l- naphthonitrile was substituted with 5-bromo-2-hydroxybenzonitrile 1. 1H NMR (400 MHz, d6-
DMSO): 8.87 (s, IH). 8.27 (d, IH), 8.05 (d, IH), 7.98 (d, IH), 7.74 (t, IH), 7.58 (br s , IH), 7.54 (t, IH), 7.48 (br s, IH), 7.42 (d,lH). MS (EI) for Ci3 Hi0N2 O2: 227 (MH+). l-Aminonaphthor2,l-blfuran-2-carboxamide [00696] l-Aminonaphtho[2,l-b]furan-2-carboxamide was synthesized in a manner similar to 3-amino-5-bromobenzofuran-2-carboxamide 3 wherein 2-(l- cyanonaphthalen-2-yloxy)acetamide was substituted with 2-(4-bromo-2- cyanophenoxy)acetamide 2. 1H NMR (400 MHz, d6-DMSO): 8.64 (d, IH), 8.36 (d,
IH), 7.85 (br s, 2H), 7.67 (dd, 2H), 7.65 (d, IH), 7.56 (s, IH), 7.36 (br s, 2H). MS
(EI) for C13 Hio N2 O2: 227 (MH+).
(Compound 96)
10-(2-Chlorophenvnnaphthorr.2':4.51furor3.2-dlpyrimidin-8(9H)-one [00697] 10-(2-Chlorophenyl)naphtho[r,2':4,5]furo[3,2-d]pyrimidin-8(9H)-one was synthesized in a manner similar to 8-bromo-2-(3-chlorophenyl)[l]benzofuro[3,2- d]pyrimidin-4(3H)-one, Example 35 wherein l-aminonaphtho[2,l-b]furan-2- carboxamide was substituted with 3-amino-5-bromobenzofuran-2-carboxamide 3, and 2-chlorobenzaldehyde was substituted with 4-bromobenzaldehyde. 1H NMR (400 MHz, de-DMSO): 10.72 (s, IH), 8.31 (d, IH), 8.23 (s, IH), 7.98 (s, IH), 7.79 (dd, IH), 7.68 (dd, IH), 7.66 (d, IH), 7.58 (m, IH), 7.52 (m, IH). MS (EI) for C20 Hn Cl N2 O2: 348 (MH+).
EXAMPLE 36
(Compound 157) 8-Bromo-2-piperidin-4-viπibenzofuror3,2-dlpyrimidin-4(3H)-one
Figure imgf000311_0001
Figure imgf000311_0002
1 , 1 -Dimethylethyl 4-(chlorocarbonyl)piperidine- 1 -carboxylate [00698] Sodium methoxide (0.706 g, 13.08 mmoles) was added in one lot to a stirred solution of N-Boc-isonipecotic acid (3 g, 13.08 mmoles, commercially available from Chem-Impex International, Inc,) in anhydrous THF (40 ml). The resulting suspension was stirred for 1 hour at room temperature, concentrated at reduced pressure and the resulting solid was suspended in anhydrous dichloromethane (20 ml). 3 drops of anhydrous DMF was added to this stirred suspension followed by the drop-wise addition of oxalyl chloride (13.08 mmoles, 1.66 g, 1.0 uL. Upon completion of addition, the reaction mixture was stirred for 2 hours, concentrated under reduced pressure, and the resulting residue of 1,1 -dimethylethyl A- (chlorocarbonyl)piperidine-l -carboxylate was diluted with anhydrous dichloromethane (40 ml) and used directly in the next step without any further purification.
1 , 1 -Dimethylethyl 4-(5-bromo-2-carbamoylbenzofuran-3-ylcarbamoyl)piperidine- 1 - carboxylate [00699] The previously made solution of 1,1 -dimethylethyl 4-
(chlorocarbonyl)piperidine-l -carboxylate (13.08 mmole) in anhydrous dichloromethane (40 ml) was added to a solution of 3-amino-5-bromobenzofuran-2- carboxamide 3 (11.77 mmoles, 2.99 g) and anhydrous pyridine (39.24 mmoles, 0.978 g, 2.12 ml) in anhydrous dichloromethane (100 ml)m maintaining the temperature between 0 - 5 0C. The reaction mixture was then transferred to a separatory funnel and washed with IM hydrochloric acid (100 ml), water (100 ml), saturated aqueous sodium bicarbonate solution (100 ml) and saturated sodium chloride solution (100 ml). The dichloromethane solution was dried over anhydrous magnesium sulfate, filtered and evaporated under reduced pressure to give a 3.45 g of white solid as crude 1,1 -dimethylethyl 4-(5 -bromo-2-carbamoylbenzofuran-3 -ylcarbamoyl)piperidine- 1 - carboxylate which was used in the next step without any further purification. 1H NMR (400 MHz, de-DMSO): 10.22 (s, IH), 8.20 (d, IH), 8.18 (br s, IH), 7.65 (br s ,1H), 7.64 (d, IH), 7.56 (d, IH), 4.06 (m. 2H), 2.89 (m, IH), 2.86 (m, 2H), 1.90 (d, 2H), 1.69 (m, 2H), 1.42 (s, 9H). MS (EI) for C20 H24 Br N3 O5: 467 (MH+). 1,1-Dimethylethyl 4-(8-bromo-4-oxo-3,4-dihydrobenzofuror3,2-dlpyrimidin-2- vDpiperidine- 1 -carboxylate
[00700] Aqueous IM sodium hydroxide (3 equivalents, 22.5 ml) was added to a stirred solution of the crude 1,1 -dimethylethyl 4-(5-bromo-2-carbamoylbenzofuran-3- ylcarbamoyl) piperidine-1 -carboxylate (7.33 mmoles, 3.42 g) and ethanol (100 ml). The resulting stirred solution was the heated to 75 0C for 3 hours. The reaction mixture was allowed to cool to room temperature and acidified with ice cold IM hydrochloric acid (pH < 3) was slowly added which resulted in the formation of a white precipitate. The slurry was stirred for 30 minutes. The precipitate was then filtered off, washed with water (50 ml), ethyl acetate (2 x 25ml) and dried under reduced pressure to give 2.78 g of 1,1 -dimethylethyl 4-(8-bromo-4-oxo-3,4- dihydrobenzofuro[3,2-d]pyrimidin-2-yl)piperidine-l -carboxylate which was used in the next step without any further purification. 1H NMR (400 MHz, d6-DMSO): 12.89 (br s, IH), 8.18 (s, IH), 7.81 (s, 2H), 4.06 (m. 2H), 2.89 (m, IH), 2.86 (m, 2H), 1.90 (d, 2H), 1.69 (m, 2H), 1.42 (s, 9H). MS (EI) for C20 H22 Br N3 O4: 448 (MH+).
(Compound 157)
8-Bromo-2-piperidin-4-viπibenzofuror3,2-dlpyrimidin-4(3H)-one [00701] The crude l,l-dimethylethyl 4-(8-bromo-4-oxo-3,4-dihydrobenzofuro[3,2- d]pyrimidin-2-yl)piperidine-l-carboxylate ( 4.77 mmoles, 2.14 ) was suspended in methanol (80 ml) and ethyl acetate (20 ml). 4M hydrogen chloride in 1,4-dioxane (23 .85 mmoles, 6 ml, commercially available from Sigma- Aldrich) was added to the stirred suspension over 2 minutes. The solid dissolved into solution. The stirred reaction mixture was then heated at 75 0C and the progress of the Boc deprotection monitored by LC-MS. After 3 hours the reaction was completed and a white precipitate was observed. The reaction mixture was allowed to cool to room temperature and the solid filtered off, washed with ethyl acetate (20 ml), diethyl ether (20 ml) and dried under reduced pressure to give 1.46 g of 8-bromo-2-piperidin-4- yl[l]benzofuro[3,2-d]pyrimidin-4(3H)-one as its dihydrochloride salt. 1H NMR (400 MHz, de-DMSO): 13.00 (broad s, IH), 8.13 (dd, IH), 8.33 (d, IH), 7.82 (d, IH), 3.98 (m, 2H), 3.03 (m, IH), 2.97 (m, 2H), 2.12 (m, 2H), 2.04 (m, 2H). MS (EI) for Ci5 Hi4 Br N3 O2: 349 (MH+).
(Compound 158) 8-Bromo-2-piperidin-3-yl[llbenzofuro[3,2-dlpyrimidin-4(3H)-one
[00702] 8-Bromo-2-piperidin-3-yl[l]benzofuro[3,2-d]pyrimidin-4(3H)-one was synthesized in a manner similar to Example 36 wherein nipecotic acid (commercially available from Chem-Impex International, Inc,) was substituted with isonipecotic acid, followed by cyclization and removal of the Boc group. 1 , 1 -Dimethylethyl 3 -(chlorocarbonyDpiperidine- 1 -carboxylate
[00703] 1 , 1 -Dimethylethyl 3 -(chlorocarbonyDpiperidine- 1 -carboxylate was synthesized in a similar manner as 1,1 -dimethylethyl 4-(chlorocarbonyl)piperidine-l- carboxylate nipecotic acid (commercially available from Chem-Impex International, Inc,) was substituted with isonipecotic acid. This material was used directed without further purification.
1 , 1 -Dimethylethyl 3 -(5 -bromo-2-carbamoylbenzofuran-3 -ylcarbamovDpiperidine- 1 - carboxylate [00704] 1,1-Dimethylethyl 3-(5-bromo-2-carbamoylbenzofuran-3- ylcarbamoyl)piperidine-l-carboxylate was synthesized in a similar manner as to 1,1- dimethylethyl 4-(5 -bromo-l-carbamoylbenzofuran-S -ylcarbamoyl)piperidine- 1 - carboxylate wherein 1,1-dimethylethyl 3-(chlorocarbonyl)piperidine-l-carboxylate was substituted with 1 , 1 -dimethylethyl 4-(chlorocarbonyl)piperidine- 1 -carboxylate. 1H NMR (400 MHz, d6-DMSO): 10.22 (s, IH), 8.20 (d, IH), 8.18 (br s, IH), 7.65 (br s ,1H), 7.64 (d, IH), 7.56 (d, IH), 3.84 (m, IH), 2.87 (m, IH), 2.62 (m, IH), 2.30 (m, IH), 2.04 (m, IH), 1.91 (m, IH), 1.72 (m, 2H), 1.62 (m, 2H), 1.35 (s, 9H). MS (EI) for C20 H24 Br N 3 O5: 467 (MH+). 1 , 1 -Dimethylethyl 3 -(8-bromo-4-oxo-3 ,4-dihydrobenzofuro [3 ,2-d]pyrimidin-2- vDpiperidine- 1 -carboxylate
[00705] 1,1-Dimethylethyl 3-(8-bromo-4-oxo-3,4-dihydrobenzofuro[3,2- d]pyrimidin-2-yl)piperidine-l -carboxylate was synthesized in a similar manner as to 1,1-dimethylethyl 4-(8-bromo-4-oxo-3,4-dihydrobenzofuro[3,2-d]pyrimidin-2- yl)piperidine-l -carboxylate where 1,1-dimethylethyl 3 -(5 -bromo-2- carbamoylbenzofuran-3-ylcarbamoyl)piperidine-l -carboxylate was substituted with 1 , 1 -dimethylethyl 4-(5-bromo-2-carbamoylbenzofuran-3-ylcarbamoyl)piperidine- 1 - carboxylate. 1H NMR (400 MHz, d6-DMSO): 12.90 (br s, IH), 8.16 (s, IH), 7.82 (d, 2H), 40.06 (m, IH), 3.87 (m, 2H), 2.88 (m, 2H), 2.50 (m, 2H), 1.81 (m, 2H), 1.37 (s, 9H). MS (EI) for C20 H22 Br N3 O4: 448 (MH+).
8-Bromo-2-piperidin-3-virilbenzofuror3,2-dlpyrimidin-4(3H)-one [00706] 8-Bromo-2-piperidin-3-yl[l]benzofuro[3,2-d]pyrimidin-4(3H)-one was synthesized in a similar manner to 8-Bromo-2-piperidin-4-yl[l]benzofuro[3,2- d]pyrimidin-4(3H)-one Example 36 wherein 1,1-dimethylethyl 4-(8-bromo-4-oxo- 3,4-dihydrobenzofuro [3, 2-d]pyrimidin-2-yl)piperidine-l -carboxylate was replaced by 1,1-dimethylethyl 3-(8-bromo-4-oxo-3,4-dihydrobenzo furo[3,2-d]pyrimidin-2- yl)piperidine-l -carboxylate. 1U NMR (400 MHz, d6-DMSO): 13.18 (br s, IH), 8.25 (s, IH), 7.83 (s, 2H), 4.23 (br s, 2H), 3.38 (m, 3H), 2.99 (m, IH), 2.14 (m, IH), 1.77 (m, 4H). MS (EI) for Ci5 Hi4 Br N3 O2: 349 (MH+). (Compound 217)
N-[4-(8-bromo-4-oxo-3,4-dihydro[llbenzofuro[3,2-dlpyrimidin-2-yl)-3- chlorophenyl1-2-prperidin-4-ylacetamide [00707] N-[4-(8-bromo^-oxo-3,4-dihydro[l]benzofiuO[3,2-d]pyrimidin-2-yl)-3- chlorophenyl]-2-piperidin-4-ylacetamide was synthesized in a manner similar to (Compound 198) wherein 2-(4-amino-2-chlorophenyl)-8-bromo[l]benzofuro[3,2- d]pyrimidin-4(3H)-one was substituted with 3-amino-5-bromobenzofuran-2- carboxamide, and 2-(l-(te/t-butoxycarbonyl)piperidin-4-yl)acetic acid was substituted with boc-3-azetidine carboxylic acid. IH NMR (400 MHz, d6-DMSO): 13.36 (s, IH), 10.62 (s, IH), 8.81 (s, br, IH), 8.65 (s, br, IH), 8.24 (s, IH), 8.03 (s, IH), 7.87 (m, 2H), 7.64 (s, 2H), 3.25 (d, 2H), 2.89 (m, 2H), 2.38 (d, 2H), 2.08 (s, br, IH), 1.83 (d, 2H), 1.44 (m, 2H). MS (EI) for C23 H20 Br Cl N4 03: 517 (MH+). (Compound 384)
8-bromo-2-[phenyl(piperazin- 1 -yDmethyl] [ 1 ]benzofuro[3 ,2-dlpyrimidin-4(3H)-one [00708] 8-bromo-2-[phenyl(piperazin- 1 -yl)methyl] [ 1 ]benzofuro[3 ,2-d]pyrimidin- 4(3H)-one was synthesized in a manner similar to Example 36 wherein 2-(4-(tert- butoxycarbonyl)piperazin-l-yl)-2-phenylacetic acid was substituted with N-boc- isonipecotic acid. IH NMR (400 MHz, d6-DMSO): 8.22 (s, 2H), 8.16 (s, IH), 7.78 (s, IH), 7.59 (d, IH), 7.32 (m, 2H), 3.17 (s, IH), 3.04 (m, 4H), 2.56 (m, 2H), 2.46 (m, 2H), 1.24 (s, IH). MS (EI) for C21 H19 Br N4 02: 440 (MH+).
EXAMPLE 36B (Compound 184) 8-bromo-2-(l-methvbiperidin-3-yl)rilbenzofuror3,2-dlpyrimidin-4(3H)-one
Figure imgf000315_0001
[00709] 8-Bromo-2-piperidin-3-yl[l]benzofuro[3,2-d]pyrimidin-4(3H)-one dihydrochloride (Compound 158) (1 equivalent, 0.205 g, 0.486 mmoles) was added to anhydrous DMF (15 ml) and stirred to ensure complete dissolution of the solid. The stirred reaction mixture was treated with 37 % aqueous formaldehyde (10 equivalents, 4.867mmoles, 0.168g, 0.4 ml) and glacial acetic acid (20 ul). The reaction mixture was stirred at room temperature for 5 minutes. The stirred reaction mixture was then treated with sodium triacetoxyborohydride (10 equivalents, 4.86 mmoles, 1.188 g, commercially available from BASF Corporation ) and additional glacial acetic acid (20 ul). The reaction mixture was then stirred at room temperature for 30 minutes and shown to be complete by LC-MS. The solution was filtered through a Millipore Millex-GN 0.20 uM Nylon syring filter, and the resulting solution was submitted for preparative reverse phase HPLC (reverse-phase, acetonitrile/water with 0.01% ammonium acetate). The resulting fractions containing the desired peak (of correct molecular weight) were combined and evaporated under reduced pressure to give 0.073 g of 8-bromo-2-(l-methylpiperidin-3-yl)[l]benzofuro[3,2-d]pyrimidin-4(3H)- one (acetate salt) as a white solid. 1U NMR (400 MHz, d6-DMSO): 13.14 (br s, IH), 8.10 (d, IH), 7.85 (d, 2H), (br s, 2H), 3.70 (m, 2H), 2.92 (m, 4H), 2.50 (s, 3H), 2.19 (m, IH), 1.94 (s, 3H). MS (EI) for Ci6 Hi6 Br N3 O2: 363 (MH+).
(Compound 185)
8-Bromo-2-(l-methylpiperidin-4-yl)πibenzofuror3,2-dlpyrimidin-4(3H)-one
[00710] 8-Bromo-2-(l-methylpiperidin-4-yl)[l]benzofuro[3,2-d]pyrimidin-4(3H)- one was synthesized in a manner similar to 8-bromo-2-(l-methylpiperidin-3- yl)[l]benzofuro[3,2-d]pyrimidin-4(3H)-one wherein 8-bromo-2-piperidin-4- yl[l]benzofuro[3,2-d]pyrimidin-4(3H)-one dihydrochloride was substituted with 8- bromo-2-piperidin-3-yl[l]benzofuro [3,2-d]pyrimidin-4(3H)-one dihydrochloride (Compound 184). 1H NMR (400 MHz, d6-DMSO): 13.00 (broad s, IH), 8.13 (dd, IH), 8.33 (d, IH), 7.82 (d, IH), 3.98 (m, 2H), 3.03 (m, IH), 2.97 (m, 2H), 2.50 (s, 3H), 2.12 (m, 2H), 2.04 (m, 2H). 1.94 (s, 3H). MS (EI) for Ci6 Hi6 Br N3 O2 : 363 (MH+).
EXAMPLE 37 (Compound 198) 2-Azetidin-3-yl-8-bromo[llbenzofuro[3,2-dlpyrimidin-4(3H)-one
Reference for the amide formation with cyanuric chloride: Org. Process Res.Dev., (1999), 3, 12
Figure imgf000317_0001
1 , 1 -Dimethylethyl 3 -(5 -bromo-2-carbamoylbenzofuran-3 -ylcarbamoyDazetidine- 1 - carboxylate
[00711] Cyanuric chloride (0.33 equivalents based on Boc-3-azetidine carboxylic acid, 4.14 mmoles, 0.763 g, commercially available from Sigma-Aldrich) was then added in one lot to a solution of Boc-3-azetidine carboxylic acid (2.50 g, 12.42 mmoles, 1 equivalent, commercially available from CNH Technologies Inc.,) in anhydrous DMA (75 ml). The mixture was stirred for 5 minutes to allow for dissolution, followed by the rapid addition of N-methylmorpholine (1.02 equivalents, 12.67 mmoles, 1.28 g, 1.39 mL). The reaction mixture was then stirred at room temperature. After 5 minutes after addition of the N-methylmorpholine, a solid was formed. The reaction mixture was then stirred at room temperature for 1 hour. 3- Amino-5-bromobenzofuran-2-carboxamide 3 (1.05 equivalents, 13.045 mmoles, 2.94 g) was then added to the stirred reaction mixture in one lot, and the reaction mixture was stirred for 16 hours. The reaction mixture was transferred to a 1000 ml separatory funnel and diluted with ethyl acetate (400 ml) and IM hydrochloric (400 ml), agitated and the ethyl acetate layer was collected. The aqueous layer was further washed with ethyl acetate (3 x 250 ml). The combined ethyl acetate solution was then washed with 0.5 M sodium hydroxide (400 ml), water (400 ml) and saturated sodium chloride solution (400 ml). The organic solution was then dried over anhydrous magnesium sulfate, filtered and evaporated under reduced pressure to give 1,1- dimethylethyl 3 -(5 -bromo-2-carbamoylbenzofuran-3 -ylcarbamoyl)azetidine- 1 - carboxylate_as the major product and was used in the next step without any purification. MS (EI) for Ci8 H20 Br N3 O5: 439 (MH+) 1 , 1 -Dimethylethyl 3 -(8-bromo-4-oxo-3 ,4-dihydrobenzofuro [3 ,2-d]pyrimidin-2- yDazetidine- 1 -carboxylate
[00712] l,l-Dimethylethyl 3-(8-bromo-4-oxo-3,4-dihydrobenzofuro[3,2- d]pyrimidin-2-yl)azetidine-l -carboxylate was synthesized in a similar manner as to 1,1 -dimethylethyl 4-(8-bromo-4-oxo-3,4-dihydrobenzofuro[3,2-d]pyrimidin-2- yl)piperidine- 1 -carboxylate wherein 1 , 1 -dimethylethyl 3 -(5 -bromo-2- carbamoylbenzofuran-3-ylcarbamoyl)azetidine-l -carboxylate was substituted with 1 , 1 -dimethylethyl 4-(5-bromo-2-carbamoylbenzofuran-3-ylcarbamoyl)piperidine- 1 - carboxylate. 1H (400 MHz, d6-DMSO): 12.96 (br s, IH), 8.23 (s, IH), 7.82 (s, 2H), 3.88 (m, IH), 3.36 (m, 4H), 1.40 (s, 9H). MS (EI) for Ci8 Hi8 Br N3 O4: 339 (MH+)
(Compound 198)
2-Azetidin-3-yl-8-bromo[llbenzofuro[3,2-dlpyrimidin-4(3H)-one [00713] 2-Azetidin-3-yl-8-bromo[l]benzofuro[3,2-d]pyrimidin-4(3H)-one was synthesized in a similar manner to 8-bromo-2-piperidin-4-yl[l]benzofuro[3,2- d]pyrimidin-4(3H)-one (Compound 157) wherein 1,1 -dimethylethyl 4-(8-bromo-4- oxo-3 ,4-dihydrobenzofuro [3 ,2-d]pyrimidin-2-yl)piperidine- 1 -carboxylate was replaced by 1,1 -dimethylethyl 3-(8-bromo-4-oxo-3,4-dihydrobenzofuro[3,2- d]pyrimidin-2-yl)azetidine-l -carboxylate. 1H NMR (400 MHz, d6-DMSO): 13.07 (br s, IH), 9.25 (br s, IH), 8.22 (dd, IH), 7.87 (d, IH), 7.86 (dd, IH), 4.37 (m, 2H), 4.21 (m, 2H), 4.16 (m, IH). MS (EI) for Ci3 Hi0 Br N3 O2: 321 (MH+).
(Compound 221)
8-Bromo-2-(l-methylazetidin-3-yl)[llbenzofuro[3,2-dlpyrimidin-4(3H)-one
[00714] 8-Bromo-2-(l-methylazetidin-3-yl)[l]benzofuro[3,2-d]pyrimidin-4(3H)- one was synthesized in a manner similar to 8-bromo-2-(l-methylpiperidin-3- yl)[l]benzofuro[3,2-d]pyrimidin-4(3H)-one wherein 2-azetidin-3-yl-8-bromo [l]benzofuro[3,2-d]pyrimidin-4(3H)-one_(Compound 198) dihydrochloride was substituted with 8-bromo-2-piperidin-3-yl[l]benzofuro[3,2-d]pyrimidin-4(3H)-one dihydrochloride (Compound 184). 1H NMR (400 MHz, d6-DMSO): 13.00 (br s, IH), 8.19 (s, IH), 7.81 (s, 2H), 5.5. (br s, 2H), 3.75 (m, 3H), 3.61 (s, 2H), 2.39 (s, 3H). MS (EI) for C14 H12 Br N3 O2: 335 (MH+). (Compound 222)
1,1-Dimethylethyl [4-(8-bromo-4-oxo-3,4-dihydro[11benzofuro[3,2-d]pyrimidm-2- vDcyclohexyll carbamate
1 , 1 -Dimethylethyl 4-(5-bromo-2-carbamoylbenzofuran-3- ylcarbamoyDcyclohexylcarbamate
[00715] The intermediate 1,1 -Dimethylethyl 4-(5-bromo-2-carbamoylbenzofuran- 3-ylcarbamoyl) cyclohexylcarbamate was synthesized in a manner similar to 1,1- dimethylethyl 3 -(5 -bromo-2-carbamoylbenzofuran-3 -ylcarbamoyl)azetidine- 1 - carboxylate, wherein 4-(tert-butoxycarbonylamino) cyclo-hexanecarboxylic acid (commercially available from CNH Technologies, Inc.) replaced Boc-3-azetidine carboxylic acid. The crude material from this reaction was submitted to the next step without any further purification. MS (EI) for C21 H26 Br N 3 O5: 481 (MH+).
1,1-Dimethylethyl r4-(8-bromo-4-oxo-3,4-dihvdrorilbenzofuror3,2-dlpyrimidin-2- vDcyclohexyll carbamate (Compound 222) [00716] 1,1-Dimethylethyl [4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2- d]pyrimidin-2-yl)cyclohexyl] carbamate was synthesized in a similar manner as to 1,1- dimethylethyl 4-(8-bromo-4-oxo-3,4-dihydrobenzofuro[3,2-d]pyrimidin-2- yl)piperidine- 1 -carboxylate wherein 1 , 1 -dimethylethyl 4-(5 -bromo-2- carbamoylbenzofuran-3-ylcarbamoyl)cyclohexylcarbamate_was substituted for 1,1- dimethylethyl 4-(5 -bromo-2-carbamoylbenzofuran-3 -ylcarbamoyl)piperidine- 1 - carboxylate. 1H NMR (400 MHz, d6-DMSO): 12.96 (br s, IH), 8.23 (s, IH), 8.03 (br s , IH), 7.82 (s, 2H), 3.88 (m, 1H),2.O1, (m, 2H), 1.87 (m, 2H), 1.73 (m, 2H), 1.65 (m, 2H), 1.43 (s, 9H). MS (EI) for C21 H24 Br N3 O4 : 463 (MH+).
(Compound 223) 2-(4-Aminocyclohexyl)-8-bromo[llbenzofuro[3,2-dlpyrimidin-4(3H)-one
[00717] 2-(4-Aminocyclohexyl)-8-bromo[l]benzofuro[3,2-d]pyrimidin-4(3H)-one was synthesized in a similar manner to 8-bromo-2-piperidin-4-yl[l]benzofuro[3,2- d]pyrimidin-4(3H)-one (Compound 157) wherein 1,1 -dimethylethyl 4-(8-bromo-4- oxo-3 ,4-dihydrobenzofuro [3 ,2-d]pyrimidin-2-yl)piperidine- 1 -carboxylate was substituted with 1,1 -dimethylethyl [4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2- d]pyrimidin-2-yl)cyclohexyl]carbamate (Compound 222). 1H NMR (400 MHz, d6- DMSO): 12.92 (br s, IH), 8.18 (s, IH), 7.83 (s, 2H), 4.31 (br s, 3H), 3.28 (m, IH), 2.92 (m, IH), 2.09 (m, 2H), 1.97 (m, 2H), 1.83 (m, 4H). MS (EI) for Ci6 Hi6 Br N3 O2 :362 (MH+).
(Compound 273) 8-Bromo-2-(l-methylpyrrolidin-2-yl)rilbenzofuror3,2-dlpyrimidin-4(3H)-one [00718] 8-Bromo-2-(l-methylpyrrolidin-2-yl)[l]benzofuro[3,2-d]pyrimidin-4(3H)- one was synthesized in a manner similar to 8-bromo-2-(l-methylpiperidin-3- yl)[l]benzofuro[3,2-d]pyrimidin-4(3H)-one wherein 2-(4-aminocyclohexyl)-8- bromo[l]benzofuro[3,2-d]pyrimidin-4(3H)-one (Compound 177) was substituted with 8-bromo-2-piperidin-3-yl[l]benzofuro[3,2-d]pyrimidin-4(3H)-one dihydrochloride (Compound 138). 1H NMR (400 MHz, d6-DMSO): 13.02 (broad s, IH), 8.22 (dd, IH), 7.82 (dd, 2H), 3.70 (m, IH), 3.46 (m, 2H), 3.14 (m, 2H), 2.72 (s, 3H), 2.37 (m, IH), 2.31 (m, IH). MS (EI) for Ci5 H14 Br N3 O2: 349 (MH+).
(Compound 297)
1,1 -Dimethylethyl 2-(8-bromo-4-oxo-3,4-dihydro[11benzofuro[3,2-d]pyrimidin-2- yl)morpholine-4-carboxylate
1 , 1 -Dimethylethyl 2-(5-bromo-2-carbamoylbenzofuran-3-ylcarbamoyl)morpholine-4- carboxylate [00719] The intermediate 1,1 -Dimethylethyl 2-(5-bromo-2-carbamoylbenzofuran-
3-ylcarbamoyl)morpholine-4-carboxylate was synthesized in a manner similar to 1,1- dimethylethyl 3 -(5 -bromo-2-carbamoylbenzofuran-3 -ylcarbamoyl)azetidine- 1 - carboxylate wherein 4-Boc morpholine-2-carboxylic acid (commercially available from PharmaCore) was substituted with Boc-3-azetidine carboxylic acid. The crude material from this reaction was submitted to the next step without any further purification. MS (EI) for Ci9 H22 Br N3 O6: 451 (MH+). 1,1-Dimethylethyl 2-(8-bromo-4-oxo-3,4-dihvdrori1benzofuror3,2-d1pyrimidin-2- yl)morpholine-4-carboxylate
[00720] 1,1 -Dimethylethyl 2-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2- d]pyrimidin-2-yl)morpholine-4-carboxylate (Compound 297) was synthesized in a similar manner as to 1,1 -dimethylethyl 4-(8-bromo-4-oxo-3,4-dihydrobenzofuro[3,2- d]pyrimidin-2-yl)piperidine-l -carboxylate wherein 1,1 -dimethylethyl 2-(5-bromo-2- carbamoylbenzofuran-3-ylcarbamoyl)morpholine-4-carboxylate was substituted with 1 , 1 -dimethylethyl 4-(5-bromo-2-carbamoylbenzofuran-3-ylcarbamoyl)piperidine- 1 - carboxylate. 1U NMR (400 MHz, d6-DMSO): 12.94 (br s, IH), 8.21 (s, IH), 7.84 (dd, 2H), 4.47 (dd, IH), 4.11 (br s, IH), 3.98 (d, IH), 3.67 (d, IH), 3.57 (td, IH), 3.21 (m, IH), 3.05 (m, IH), 1.43 (s, 9H). MS (EI) for Ci9 H20 Br N3 O5: 451 (MH+).
(Compound 298) 8-Bromo-2-morpholin-2-viπibenzofuror3,2-dlpyrimidin-4(3H)-one
[00721] 8-Bromo-2-morpholin-2-yl[l]benzofuro[3,2-d]pyrimidin-4(3H)-one was synthesized in a similar manner to 8-bromo-2-piperidin-4-yl[l]benzofuro[3,2- d]pyrimidin-4(3H)-one (Compound 157) wherein 1,1-dimethylethyl 4-(8-bromo-4- oxo-3 ,4-dihydrobenzofuro [3 ,2-d]pyrimidin-2-yl)piperidine- 1 -carboxylate was substituted with 1,1-dimethylethyl 2-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2- d]pyrimidin-2-yl)morpholine-4-carboxylate (Compound 297). 1H NMR (400 MHz, d6-DMSO):13.20 (br s, IH), 8.25 (d, IH), 7.86 (d, IH), 7.85 (d, IH), 4.93 (dd, IH), 4.08 (dd, IH), 3.85 (t, IH), 3.56 (dd, IH), 3.47 (m IH), 2.27 (d, IH), 3.12 (m, IH). MS (EI) for C19 H20 Br N3 O5: 351 (MH+). (Compound 299)
8-Bromo-2-r3-(piperidin-4-yloxy)isoxazol-5-ylirilbenzofuror3,2-dlpyrimidin-4(3H)- one
1 , 1 -Dimethylethyl 4-(5-(5-bromo-2-carbamoylbenzofuran-3-ylcarbamoyl)isoxazol-3- yloxy)piperidine- 1 -carboxylate [00722] 1,1 -Dimethylethyl 4-(5-(5-bromo-2-carbamoylbenzofuran-3- ylcarbamoyl)isoxazol-3-yloxy)piperidine-l -carboxylate was synthesized in a manner similar to 1,1-dimethylethyl 3-(5-bromo-2-carbamoylbenzofuran-3- ylcarbamoyl)azetidine- 1 -carboxylate wherein 3 -( 1 -(tert-butoxycarbonyl)piperidin-4- yloxy)isoxazole-5-carboxylic acid (prepared from a literature procedure WO2002051849) was substituted with Boc-3-azetidine carboxylic acid. The crude material from this reaction was submitted to the next step without any further purification. MS (EI) for C23 H25 Br N4 O7: 555 (MH+).
1,1-Dimethylethyl 4-(5-(8-bromo-4-oxo-3,4-dihydrobenzofuror3,2-dlpyrimidin-2- yl)isoxazol-3-yloxy)piperidine-l-carboxylate [00723] 1,1 -Dimethylethyl 4-(5-(8-bromo-4-oxo-3,4-dihydrobenzofuro[3,2- d]pyrimidin-2-yl)isoxazol-3-yloxy)piperidine-l -carboxylate was synthesized in a similar manner as to 1,1-dimethylethyl 4-(8-bromo-4-oxo-3,4-dihydrobenzofuro[3,2- d]pyrimidin-2-yl)piperidine-l -carboxylate wherein 1,1-dimethylethyl 4-(5-(5- bromo-2-carbamoylbenzofuran-3 -ylcarbamoyl)isoxazol-3 -yloxy)piperidine- 1 - carboxylate was substituted with 1,1-dimethylethyl 4-(5-bromo-2- carbamoylbenzofuran-3-ylcarbamoyl)piperidine-l -carboxylate. The crude material from this reaction was used in the subsequent step without any further purification. MS (EI) for C23 H23 Br N4 O: 532 (MH+).
8-Bromo-2-[3-(piperidin-4-yloxy)isoxazol-5-yll[llbenzofuro[3,2-dlpyrimidin-4(3H)- one (Compound 299)
[00724] 8-Bromo-2-[3-(piperidin-4-yloxy)isoxazol-5-yl][l]benzofuro[3,2- d]pyrimidin-4(3H)-one as its hydrochloride salt, was synthesized in a similar manner to 8-bromo-2-piperidin-4-yl[l]benzofuro[3,2-d]pyrimidin-4(3H)-one (Compound 157) wherein 1,1-dimethylethyl 4-(8-bromo-4-oxo-3,4-dihydrobenzofuro[3,2- d]pyrimidin-2-yl)piperidine-l -carboxylate was substituted with 1,1-dimethylethyl 4- (5-(8-bromo-4-oxo-3,4-dihydrobenzofuro[3,2-d]pyrimidin-2-yl)isoxazol-3- yloxy)piperidine-l -carboxylate. 1H NMR (400 MHz, d6-DMSO): 13.69 (br s, IH), 8.98 (br s, 2H), 8.26 (s IH), 7.88 (m, 2H), 7.24 (s, IH), 4.95 (m, IH), 3.26 (m, 2H), 3.11 (m, 2H), 2.23 (m, 2H), 1.95 (m, 2H). MS (EI) for C18 Hi5 Br N4 O4: 432 (MH+).
(Compound 506)
(S)- 1 , 1 ,-dimethylethyl-2-(2-carbamoylbenzofuran-3-ylcarbamoyl)pyrrolidine- 1 - carboxylate
[00725] The intermediate (S)-1 , 1 ,-dimethylethyl-2-(2-carbamoylbenzofuran-3- ylcarbamoyl)pyrrolidine-l -carboxylate was synthesized in a manner similar to 1,1- dimethylethyl 3 -(5 -bromo-2-carbamoylbenzofuran-3 -ylcarbamoyl)azetidine- 1 - carboxylate 4-(tert-butoxycarbonylamino), using 3-aminobenzofuran-2-carboxamide (commercially available from Sigma- Aldrich) and N-Boc-L-proline (commercially available from Chemlmpex International Inc.,) which were substituted with 5-bromo- 3-aminobenzofuran-2-carboxamide and Boc-3-azetidine carboxylic acid, respectively. The crude material from this reaction was submitted to the next step without any further purification. MS (EI) for C19 H23 N3 05: 374 (MH+). lJ-Dimethylethyl-2-r(2S)-pyrrolidin-2-ylirilbenzofuror3.2-dlpyrimidin-4(3H)-one [00726] 1,1 -Dimethylethyl-2- [(2S)-pyrrolidin-2-yl] [ 1 ]benzofuro [3 ,2-d]pyrimidin- 4(3H)-one was synthesized in a similar manner to 1,1-dimethylethyl 4-(8-bromo-4- oxo-3 ,4-dihydrobenzofuro [3 ,2-d]pyrimidin-2-yl)piperidine- 1 -carboxylate wherein (S)- 1 , 1 ,-dimethylethyl-2-(2-carbamoylbenzofuran-3-ylcarbamoyl)pyrrolidine- 1 - carboxylate was substituted for 1,1-dimethylethyl 4-(5-bromo-2-carbamoylbenzofuran-3- ylcarbamoyl)piperidine-l -carboxylate. The crude material from this reaction was air dried and submitted to the next step without any further purification. 1H NMR (400 MHz, de-DMSO): 13.99 (br s IH), 8.01 (t, IH), 7.83 (d, IH), 7.87 (t, IH), 7.49 (t, IH), 4.68 (m, IH), 3.63 (m, IH), 3.44 (m, IH), 3.31 (m, IH), 2.02 (m, 2H), 1.85 (m, IH), 1.10 (s, 9H). MS (EI) for C19 H21 N3 04: 356 (MH+). 2-r(2S)-pyrrolidin-2-yll \ 1 lbenzofuror3.2-dlpyrimidin-4(3H)-one [00727] 2-[(2S)-pyrrolidin-2-yl][l]benzofuro[3,2-d]pyrimidin-4(3H)-one was synthesized in a similar manner as to 1,1-dimethylethyl 4-(8-bromo-4-oxo-3,4- dihydrobenzofuro [3 ,2-d]pyrimidin-2-yl)piperidine- 1 -carboxylate wherein (S)- 1 , 1 ,- dimethylethyl-2-(2-carbamoylbenzofuran-3 -ylcarbamoyl)pyrrolidine- 1 -carboxylate was substituted for 1,1-dimethylethyl 4-(5-bromo-2-carbamoylbenzofuran-3- ylcarbamoyl)piperidine-l -carboxylate. The crude material from this reaction was air dried and submitted to the next step without any further purification. 1H NMR (400 MHz, de-DMSO): 13.30 (br s IH), 8.03 (t, IH), 7.85 (d, IH), 7.83 (t, IH), 7.44 (t, IH), 4.793 (t, IH), 3.51 (m, IH), 3.37 (br s 2H), 3.35 (m, IH), 2.43 (m, IH), 2.15 (m, IH), 2.03 (m, IH). MS (EI) for Ci4 Hi3 N3 O2: 256 (MH+). (Compound 507)
N-r2-(Aminocarbonyl)-5-chloro- 1 -benzofuran-3-yll-L-prolinamide hydrochloride [00728] L-Proline (5.0 g, 43.4 mmol) was suspended in anhydrous dichloromethane (50 mL) and cooled in a -100C ice bath. Phosphorus pentachloride (8.14 g, 39.1 mmol) was added in one portion. The reaction mixture was stirred in the cold bath for 60 min. Another reaction flask was charged with commercially available 3-amino-5-chloro-l-benzofuran-2-carboxamide, (2.28 g, 10.8 mmol) and dichloromethane (25 mL) and the suspension was cooled in an ice bath. The solution of proline acid chloride was added dropwise to the cold suspension of 3-amino-5- chloro-l-benzofuran-2-carboxamide over a period of 5 min. The reaction mixture was allowed to warm to room temperature and was stirred overnight. The precipitate was filtered off using dichloromethane (50 mL) to transfer the solid from the reaction flask. The solid was washed with dichloromethane (3 x 20 mL) and dried under vacuum to give 4.05 g of N-[2-(aminocarbonyl)-5-chloro-l-benzofuran-3-yl]-L- prolinamide hydrochloride. The material was used without further purification. 1H NMR (400 MHz, d6-DMSO): 10.00 ( br s, IH), 8.71 (br s, IH), 8.20 (s, IH), 7.92 (s, IH), 7.82 (d, IH), 7.65 (dd, IH), 7.52 (dd, IH), 4.55 (m, IH), 3.51 (br s, 2H), 3.26 (m, 2H, 2.40 (m, IH), 2.08 (m, IH), 1.83 (m, 2H). MS (EI) for Ci4 Hi4 Cl N3 O3: 308 (MH+).
8-Chloro-2-r(2S)-pyrrolidin-2-ylirilbenzofuror3.2-dlpyrimidin-4(3H)-one hydrochloride
[00729] N-[2-(Aminocarbonyl)-5-chloro- 1 -benzofuran-3-yl]-L-prolinamide hydrochloride monohydrate (4.0 g, 11.6 mmol) was suspended in ethanol (100 mL) and sodium hydroxide (30 mL, 2.0 M, 60 mmol) was added in one portion which caused all the material to dissolve. The reaction mixture was stirred at 45°C for 4h and was allowed to cool to room temperature. The reaction flask was cooled in an ice bath (00C) and hydrochloric acid (6.0 M) was added dropwise to lower the pH to 2. The reaction mixture was removed from the ice bath and allowed to stand at 4°C in a refrigerator for 16 hours. The precipitate was isolated by filtration, washed with cold water (2 x 10 mL), and air dried for 3 h. The solid was then washed with ethyl acetate (3 x 10 mL) and dried under vacuum to give 1.97 g (59% yield, >98% purity) of 8- chloro-2-[(2S)-pyrrolidin-2-yl][l]benzofuro[3,2-d]pyrimidin-4(3H)-one hydrochloride.
1H NMR (400 MHz, d6-DMSO): 13.50 (br s IH), 8.07 (d, IH), 7.94 (d, IH), 7.74 (dd, IH), 4.793 (t, IH), 3.51 (m, IH), 3.37 (br s 2H), 3.35 (m, IH), 2.43 (m, IH), 2.15 (m, IH), 2.03 (m, IH). MS (EI) for Ci4Hi2ClN3O2: 290 (MH+). (Compound 515)
1 , 1 ,-dimethylethyl-5-(5-bromo-2-carbamoylbenzofuran-3-ylcarbamoyl)-(2S)-2,2- dimethylpyrrolidine- 1 -carboxylate
[00730] The intermediate l,l,-dimethylethyl-5-(5-bromo-2-carbamoylbenzofuran- 3-ylcarbamoyl)-(2S)-2,2-dimethylpyrrolidine-l -carboxylate was synthesized in a manner similar to 1 , 1 -dimethylethyl 3 -(5 -bromo-2-carbamoylbenzofuran-3 - ylcarbamoyl) azetidine-1 -carboxylate 4-(tert-butoxycarbonylamino), using 3-amino- 5-chlorobenzofuran-2-carboxamide and (S)-N-Boc-5,5-dimethylpyrrolidine-2- carboxylic acid (commercially available from Chemlmpex International Inc.,.) which replaced Boc-3-azetidine carboxylic acid. The crude material from this reaction was submitted to the next step without any further purification. MS (EI) for C21 H26 Br N3 O5: 481 (MH+). 1 , 1 ,-dimethylethyl-5-(8-bromo-4-oxo-3,4-dihydrobenzofuro[3,2-dlpyrimidin-2-yl)- (2S)-2,2-dimethylpyrrolidine- 1 -carboxylate
[00731] 1 , 1 ,-Dimethylethyl-5-(8-bromo-4-oxo-3,4-dihydrobenzofuro[3,2- d]pyrimidin-2-yl)-(2S)-2,2-dimethylpyrrolidine-l -carboxylate was synthesized in a similar manner as to 1,1-dimethylethyl 4-(8-bromo-4-oxo-3,4-dihydrobenzofuro[3,2- d]pyrimidin-2-yl)piperidine-l -carboxylate, wherein l,l,-dimethylethyl-5-(5-bromo- 2-carbamoylbenzofuran-3-ylcarbamoyl)-(2S)-2,2-dimethylpyrrolidine-l -carboxylate replaced 1,1-dimethylethyl 4-(5-bromo-2-carbamoylbenzofuran-3- ylcarbamoyl)piperidine-l -carboxylate. The crude material from this reaction was air dried and submitted to the next step without any further purification. MS (EI) for C21 H24 Br N3 O4: 464 (MH+).
8-bromo-2-r(2S)-5,5-dimethylpyrrolidin-2-yliπibenzofuror3,2-dlpyrimidin-4(3H)- one
[00732] 8-Bromo-2-[(2S)-5,5-dimethylpyrrolidin-2-yl][l]benzofuro[3,2- d]pyrimidin-4(3H)-one was synthesized in a similar manner to 8-bromo-2-piperidin- 4-yl[l]benzofuro[3,2-d]pyrimidin-4(3H)-one (Compound 157), wherein 1,1- dimethylethyl 4-(8-bromo-4-oxo-3,4-dihydrobenzofuro[3,2-d]pyrimidin-2- yl)piperidine-l -carboxylate was substituted with l,l,-dimethylethyl-5-(8-bromo-4- oxo-3 ,4-dihydrobenzofuro [3 ,2-d]pyrimidin-2-yl)-(2S)-2,2-dimethylpyrrolidine- 1 - carboxylate. 1H NMR (400 MHz, d6-DMSO): 8.11 (dd, IH), 7.74 (dd, 2H), 4.41 (q, IH), 3.40 (br s , 2H), 2.39 (m, IH), 2.10 (m, IH), 1.69 (t, 1.69), 1.28 (s, 3H), 1.25 (s, 3H). MS (EI) for Ci6 Hi6 Br N3 O2: 364 (MH+).
(Compound 470)
1 , 1 -dimethylethyl (2S)-2-( { [2-(aminocarbonyl)-5 -bromo- 1 -benzofuran-3 - yl] amino I carbonyDpyrrolidine- 1 -carboxylate [00733] 1,1-dimethylethyl (2S)-2-( {[2-(aminocarbonyl)-5 -bromo- 1 -benzofuran-3 - yl] amino }carbonyl)pyrrolidine-l -carboxylate was synthesized in a manner similar to Example 37, 1,1-dimethylethyl 3-(5-bromo-2-carbamoylbenzofuran-3- ylcarbamoyl)azetidine- 1 -carboxylate. N-(tert-butoxycarbonyl)-L-proline was substituted with Boc-3-azetidine carboxylic acid. The crude material from this reaction was submitted to the next step without any further purification. MS (EI) for Ci9H22BrN3O5: 452.01 (MH+).
1,1-dimethylethyl (25V2-(8-bromo-4-oxo-3Λ-dihvdrori1benzofuror32-^pyrimidin- 2-yl)pyrrolidine- 1 -carboxylate
[00734] 1,1-dimethylethyl (2S)-2-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2- <i]pyrimidin-2-yl)pyrrolidine-l -carboxylate was synthesized in a similar manner as to 1,1-dimethylethyl 3-(8-bromo-4-oxo-3,4-dihydrobenzofuro[3,2-d]pyrimidin-2- yl)azetidine- 1 -carboxylate, wherein 1 , 1 -dimethylethyl (2S)-2-( { [2-(aminocarbonyl)- 5-bromo-l-benzofuran-3-yl]amino}carbonyl)pyrrolidine-l -carboxylate replaced 1,1- dimethylethyl 4-(5-bromo-2-carbamoylbenzofuran-3-ylcarbamoyl)azetidine- 1 - carboxylate. The crude material from this reaction was submitted to the next step without any further purification. MS (EI) for Ci9H20BrN3O4: 434.06 (MH+). 8-bromo-2-[(2S)-pyrrolidin-2-yll[llbenzofuro[3,2-dlpyrimidin-4(3H)-one [00735] 8-bromo-2-[(2S)-pyrrolidin-2-yl][l]benzofuro[3,2-d]pyrimidin-4(3H)-one was synthesized in a similar manner to 8-bromo-2-piperidin-4-yl[l]benzofuro[3,2- d]pyrimidin-4(3H)-one (Compound 157), wherein 1,1-dimethylethyl 4-(8-bromo-4- oxo-3,4-dihydrobenzofuro[3,2-d]pyrimidin-2-yl)piperidine-l-carboxylate was replaced by 1,1-dimethylethyl (25)-2-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2- J]pyrimidin-2-yl)pyrrolidine-l -carboxylate. 1H NMR (400 MHz, d6-DMSO): 13.51 (s, IH), 10.39 (s, IH), 9.08 (s, IH), 8.22 (m, IH), 7.88 (m, 2H), 4.78 (m, IH), 3.50 (m, 2H), 2.45 (m, IH), 2.14 (m, IH), 2.04 (m, 2H). MS (EI) for Ci4Hi2BrN3O2: 333.92 (MH+).
(Compound 488) 1 , 1 -dimethylethyl (2S,4R)-2-( { [2-(aminocarbonyl)-5 -bromo- 1 -benzofuran-3 - yll amino I carbonvD-4-hvdroxypyrrolidine- 1 -carboxylate
[00736] 1 , 1 -dimethylethyl (2S,AR)-2-( { [2-(aminocarbonyl)-5-bromo- 1 -benzofuran- 3-yl]amino}carbonyl)-4-hydroxypyrrolidine-l -carboxylate was synthesized in a manner similar Example 37, 1,1-dimethylethyl 3-(5-bromo-2-carbamoylbenzofuran- 3 -ylcarbamoyl)azetidine- 1 -carboxylate . trans-N-(tert-butoxycarbonyl)-4-hydroxy-L- proline replaced Boc-3-azetidine carboxylic acid. The crude material from this reaction was submitted to the next step without any further purification. MS (EI) for C28H29Br2N5O8: 468.01 (MH+). l.l-dimethylethvU2S.4igV2-(8-bromo-4-oxo-3.4-dihvdroπibenzofuror3.2- J]pyrimidin-2-yl)-4-hydroxypyrrolidine- 1 -carboxylate [00737] 1,1-dimethylethyl (2S,4i?)-2-(8-bromo-4-oxo-3,4- dihydro [ 1 ]benzofuro [3 ,2-d]pyrimidin-2-yl)-4-hydroxypyrrolidine- 1 -carboxylate was synthesized in a similar manner as to 1,1-Dimethylethyl 3-(8-bromo-4-oxo-3,4- dihydrobenzofuro [3 ,2-d]pyrimidin-2-yl)azetidine- 1 -carboxylate wherein 1,1- dimethylethyl (2S,4R)-2-( { [2-(aminocarbonyl)-5 -bromo- 1 -benzofuran-3 - yl] amino } carbonyl)-4-hydroxypyrrolidine- 1 -carboxylate replaced 1 , 1 -dimethylethyl 4-(5-bromo-2-carbamoylbenzofuran-3-ylcarbamoyl)azetidine- 1 -carboxylate. 1H NMR (400 MHz, d6-DMSO): 8.20 (s, IH), 7.84 (s, 2H), 5.17 (bs, IH), 4.77 (m, IH), 4.38 (bs, IH), 3.69 (m, 2H), 2.24 (m, IH), 1.09 (s, 9H). MS (EI) for Ci9H20BrN3O5: 451.89 (MH+).
8-bromo-2-r(2S.4R)-4-hvdroxypyrrolidin-2-ylirilbenzofuror3.2-dlpyrimidin-4(3H)- one [00738] 8-bromo-2-[(2S,4R)-4-hydroxypyrrolidin-2-yl][l]benzofuro[3,2- d]pyrimidin-4(3H)-one was synthesized in a similar manner to 8-bromo-2-piperidin- 4-yl[l]benzofuro[3,2-d]pyrimidin-4(3H)-one (Compound 157), wherein 1,1- dimethylethyl 4-(8-bromo-4-oxo-3,4-dihydrobenzofuro[3,2-d]pyrimidin-2- yl)piperidine-l -carboxylate was replaced by 1,1-dimethylethyl (25',4i?)-2-(8-bromo-4- oxo-3,4-dihydro[l]benzofuro[3,2-(i]pyrimidin-2-yl)-4-hydroxypyrrolidine-l- carboxylate. 1H NMR (400 MHz, d6-DMSO): 8.05 (s, IH), 7.63 (s, 2H), 4.70 (bs, IH), 4.24 (bs, 2H), 1.94 (bs, 2H). MS (EI) for Ci4Hi2BrN3O3: 350.05 (MH+).
(Compound 300)
1 , 1 -Dimethylethyl 6-(5 -bromo-2-carbamoylbenzofuran-3 -ylcarbamoyl)-3 A- dihydroisoquinoline-2( 1 H)-carboxylate
[00739] 1 , 1 -Dimethylethyl 6-(5-bromo-2-carbamoylbenzofuran-3-ylcarbamoyl)- 3,4-dihydroisoquinoline-2(lH)-carboxylate was synthesized in a manner similar to 1 , 1 -dimethylethyl 3 -(5 -bromo-2-carbamoylbenzofuran-3 -ylcarbamoyl)azetidine- 1 - carboxylate, wherein 2-(tert-butoxycarbonyl)- 1 ,2,3 ,4-tetrahydroisoquinoline-6- carboxylic acid (commercially available from ASW MedChem, Inc.,) replaced Boc-3- azetidine carboxylic acid. The crude material from this reaction was submitted to the next step without any further purification. MS (EI) for C24 H24 Br N3 O5: 515 (MH+). 1,1-Dimethylethyl 6-(8-bromo-4-oxo-3,4-dihydrobenzofuro[3,2-dlpyrimidin-2-yl)- 3,4-dihydroisoquinoline-2(lH)-carboxylate
[00740] 1,1-Dimethylethyl 6-(8-bromo-4-oxo-3,4-dihydrobenzofuro[3,2- d]pyrimidin-2-yl)-3,4-dihydroisoquinoline-2(lH)-carboxylate was synthesized in a similar manner as to 1,1-dimethylethyl 4-(8-bromo-4-oxo-3,4-dihydrobenzofuro[3,2- d]pyrimidin-2-yl)piperidine-l-carboxylate, wherein 1,1-dimethylethyl 6-(5-bromo-2- carbamoylbenzofuran-3 -y lcarbamoyl)-3 ,4-dihydroisoquinoline-2( 1 H)-carboxylate replaced 1,1-dimethylethyl 4-(5-bromo-2-carbamoylbenzofuran-3- ylcarbamoyl)piperidine-l-carboxylate. The crude material from this reaction was used in the subsequent step without any further purification. MS (EI) for C 19 H14 Br N3 O2: 497 (MH+).
8-Bromo-2-(l,2,3,4-tetrahvdroisoquinolin-6-yl)rilbenzofuror3,2-dlpyrimidin-4(3H)- one (Compound 300)
[00741] 8-Bromo-2-(l,2,3,4-tetrahydroisoquinolin-6-yl)[l]benzofuro[3,2- d]pyrimidin-4(3H)-one as its hydrochloride salt, was synthesized in a similar manner to 8-bromo-2-piperidin-4-yl[l]benzofuro[3,2-d]pyrimidin-4(3H)-one (Compound 157), wherein 1,1-dimethylethyl 4-(8-bromo-4-oxo-3,4-dihydrobenzofuro[3,2- d]pyrimidin-2-yl)piperidine-l-carboxylate was substituted with 1,1-dimethylethyl 6- (8-bromo-4-oxo-3,4-dihydrobenzofuro[3,2-d]pyrimidin-2-yl)-3,4- dihydroisoquinoline-2(lH)-carboxylate 1U NMR (400 MHz, d6-DMSO): 13.20 (br s, IH), 9.71 (br s, 2H), 8.28 (s, IH), 8.05 (s, IHO, 8.03 (d, IH), 7.85 (m, 2H), 7.42 (d, IH), 4.35 (s, 2H), 4.41 (s, 2H), 3.12 (t, 2H). MS (EI) for Ci9 Hi4 Br N3 O2: 397 (MH+).
(Compound 301) 1,1-Dimethylethyl 2-(5-bromo-2-carbamoylbenzofuran-3-ylcarbamoyl)-5,6- dihydroimidazo[ 1 ,2-a"|pyrazine-7(8H)-carboxylate
[00742] 1 , 1 -Dimethylethyl 2-(5-bromo-2-carbamoylbenzofuran-3-ylcarbamoyl)- 5,6-dihydroimidazo[l,2-a]pyrazine-7(8H)-carboxylate was synthesized in a manner similar to 1,1-dimethylethyl 3-(5-bromo-2-carbamoylbenzofuran-3- ylcarbamoyl)azetidine-l-carboxylate, wherein 7-(tert-butoxycarbonyl)-5,6,7,8- tetrahydroimidazo[l,2-a]pyrazine-2-carboxylic acid (commercially available from J & W PharmLab LLC) replaced Boc-3-azetidine carboxylic acid. The crude material from this reaction was submitted to the next step without any further purification. MS (EI) for C2i H22 Br N5 O5: 505 (MH+).
1,1-Dimethylethyl 2-(8-bromo-4-oxo-3,4-dihvdrobenzofuror3,2-dlpyrimidin-2-yl)- 5,6-dihydroimidazori,2-alpyrazine-7(8H)-carboxylate [00743] The intermediate 1,1 -dimethylethyl 2-(8-bromo-4-oxo-3 ,4- dihydrobenzofuro[3,2-d]pyrimidin-2-yl)-5,6-dihydroimidazo[l,2-a]pyrazine-7(8H)- carboxylate was synthesized in a similar manner as to 1,1-dimethylethyl 4-(8-bromo- 4-oxo-3,4-dihydrobenzofuro[3,2-d]pyrimidin-2-yl)piperidine-l-carboxylate, wherein 1,1-dimethylethyl 2-(5-bromo-2-carbamoy-lbenzofuran-3-ylcarbamoyl)-5,6- dihydroimidazo[l,2-a]pyrazine-7(8H)-carboxylate replaced 1,1-dimethylethyl 4-(5- bromo-2-carbamoylbenzofuran-3-ylcarbamoyl)-piperidine-l-carboxylate. The crude material from this reaction was used in the subsequent step without any further purification. MS (EI) for C21 H20 Br N5 O4: 487 (MH+).
(Compound 301) 8-Bromo-2-(5,6,7,8-tetrahydroimidazo[l,2-alpyrazin-2-yl)[llbenzofuro[3,2- d"|pyrimidin-4(3H)-one
[00744] 8-Bromo-2-(5,6,7,8-tetrahydroimidazo[l,2-a]pyrazin-2- yl)[l]benzofuro[3,2-d]pyrimidin-4(3H)-one as its hydrochloride salt, was synthesized in a similar manner to 8-bromo-2-piperidin-4-yl[l]benzofuro[3,2-d]pyrimidin-4(3H)- one (Compound 157), wherein 1,1-dimethylethyl 4-(8-bromo-4-oxo-3,4- dihydrobenzofuro [3,2-d]pyrimidin-2-yl)piperidine-l-carboxylate was substituted with 1 , 1 -dimethylethyl 2-(8-bromo-4-oxo-3 ,4-dihydrobenzofuro [3 ,2-d]pyrimidin-2- yl)-5,6-dihydroimidazo[l,2-a]pyrazine-7(8H)-carboxylate. 1H NMR (400 MHz, d6- DMSO): 10.34 (br s, IH), 8.15 (m, IH), 7.83 (m, 2H), 4.78 (br s, 2H), 4.47 (s, 2H), 4.41 (t, 2H), 3.66 (s, 2H). MS (EI) for Ci6 H12 Br N5 O2: 389 (MH+).
(Compound 302)
1 , 1 -Dimethylethyl 3-(5-bromo-2-carbamoylbenzofuran-3- ylcarbamovDbenzylcarbamate
[00745] The intermediate 1 , 1 -Dimethylethyl 3 -(5 -bromo-2-carbamoylbenzofuran- 3-ylcarbamoyl)benzylcarbamate was synthesized in a manner similar to 1,1- dimethylethyl 3 -(5 -bromo-2-carbamoylbenzofuran-3 -ylcarbamoyl)azetidine- 1 - carboxylate, wherein 3-((tert-butoxycarbonylamino)-methyl)benzoic acid
(commercially available from CNH Technologies, Inc.,) replaced Boc-3-azetidine carboxylic acid. The crude material from this reaction was submitted to the next step without any further purification. MS (EI) for C22 H22 Br N3 O5: 489 (MH+). 1 , 1 -Dimethylethyl 3 -(8-bromo-4-oxo-3 ,4-dihydrobenzofuro [3 ,2-d1pyrimidin-2- vDbenzylcarbamate [00746] 1,1 -Dimethylethyl 3-(8-bromo-4-oxo-3,4-dihydrobenzofuro[3,2- d]pyrimidin-2-yl)benzylcarbamate was synthesized in a similar manner as to 1,1- dimethylethyl 4-(8-bromo-4-oxo-3,4-dihydrobenzofuro[3,2-d]pyrimidin-2- yl)piperidine- 1 -carboxylate, wherein 1 , 1 -dimethylethyl 3 -(5 -bromo-2- carbamoylbenzofuran-3-ylcarbamoyl)benzylcarbamate replaced 1,1 -dimethylethyl 4- (5-bromo-2-carbamoylbenzofuran-3-ylcarbamoyl)piperidine-l -carboxylate. The crude material from this reaction was used in the subsequent step without any further purification. MS (EI) for C22 H20 Br N3 O4: 471 (MH+).
2- [3 -(Aminomethyl)phenvH -8-bromo [ 1 lbenzofuro [3 ,2-dlpyrimidin-4(3H)- one (Compound 302) [00747] 2-[3-(Aminomethyl)phenyl]-8-bromo[l]benzofuro[3,2-d]pyrimidin-4(3H)- one as its hydrochloride salt, was synthesized in a similar manner to 8-bromo-2- piperidin-4-yl[l]benzofuro[3,2-d]pyrimidin-4(3H)-one (Compound 157) wherein 1,1 -dimethylethyl 4-(8-bromo-4-oxo-3,4-dihydrobenzofuro[3,2-d]pyrimidin-2- yl)piperidine-l -carboxylate was substituted with 1,1 -dimethylethyl 3-(8-bromo-4- oxo-3,4-dihydrobenzofuro[3,2-d]pyrimidin-2-yl)benzylcarbamate. 1H NMR (400 MHz, de-DMSO): 13.20 (br s, IH), 6.56 (br s, 3H), 8.33 (s, IH), 8.24 (d, IH), 8.15 (d, IH), 7.86 (m, 2H), 7.75 (d, IH), 7.62 (t, IH), 4.14 (q, 2H). MS (EI) for C17 H12 Br N3 O2: 370 (MH+).
(Compound 303) 1 , 1 -Dimethylethyl 4-(4-(5-bromo-2-carbamoylbenzofuran-3- ylcarbamoyDphenyDpiperazine- 1 -carboxylate [00748] The intermediate 1 , 1 -Dimethylethyl 4-(4-(5-bromo-2- carbamoylbenzofuran-3 -y lcarbamoyl) phenyl)piperazine- 1 -carboxylate was synthesized in a manner similar to 1,1 -dimethylethyl 3-(5-bromo-2- carbamoylbenzofuran-3 -y lcarbamoyl)azetidine- 1 -carboxylate, wherein 4-(4-(tert- butoxycarbonyl)piperazin-l-yl)benzoic acid (commercially available from CHESS GmbH.) replaced Boc-3-azetidine carboxylic acid. The crude material from this reaction was submitted to the next step without any further purification. MS (EI) for C25 H27 Br N4 O5: 545 (MH+).
1,1-Dimethylethyl 4-(4-(8-bromo-4-oxo-3,4-dihydrobenzofuror3,2-dlpyrimidin-2- vDphenvDpiperazine- 1 -carboxylate [00749] 1,1-Dimethylethyl 4-(4-(8-bromo-4-oxo-3,4-dihydrobenzofuro[3,2- d]pyrimidin-2-yl)phenyl)piperazine-l -carboxylate was synthesized in a similar manner as to 1,1-dimethylethyl 4-(8-bromo-4-oxo-3,4-dihydrobenzofuro[3,2- d]pyrimidin-2-yl)piperidine-l -carboxylate, using IM potassium hydroxide and isopropyl alcohol as solvent, wherein 1,1-dimethylethyl 4-(4-(5-bromo-2- carbamoylbenzofuran-3 -ylcarbamoyl)phenyl)- piperazine- 1 -carboxylate replaced 1,1- dimethylethyl 4-(5 -bromo-2-carbamoylbenzofuran-3 -ylcarbamoyl)piperidine- 1 - carboxylate. The crude product from this reaction was used in the subsequent step without any further purification. MS (EI) for C25 H27 Br N4 O5: 526 (MH+).
8-Bromo-2-(4-piperazin- 1 -ylphenyl)[ 1 lbenzofuro[3,2-dlpyrimidin-4(3H)-one (Compound 303)
[00750] 8-Bromo-2-(4-piperazin- 1 -ylphenyl)[ 1 ]benzofuro[3,2-d]pyrimidin-4(3H)- one as its hydrochloride salt, was synthesized in a similar manner to 8-bromo-2- piperidin-4-yl[l]benzofuro[3,2-d]pyrimidin-4(3H)-one (Compound 157), wherein 1,1-dimethylethyl 4-(8-bromo-4-oxo-3,4-dihydrobenzofuro[3,2-d]pyrimidin-2- yl)piperidine-l -carboxylate was substituted with 1,1-dimethylethyl 4-(4-(8-bromo-4- oxo-3 ,4-dihydrobenzofuro [3 ,2-d]pyrimidin-2-yl)phenyl)piperazine- 1 -carboxylate . 1H NMR (400 MHz, d6-DMSO): 13.02 (br s, IH), 8.27 (dd, IH), 8.15 (d, 2H), 7.84 (d, 2H), 7.13 (d, 2H), 4.98 (br s, 2H), 3.58 (t, 4H), 3.22 (br s, 4H). MS (EI) for C20 Hn Br N4 O2: 426 (MH+). (Compound 304)
1 -(I -Benzylpiperidin-4-yl)-5-oxopyrrolidine-3-carboxylic acid
Figure imgf000331_0001
[00751] A rapidly stirred mixture of itaconic acid (80 mmoles, 10.4 g, commercially available from Sigma- Aldrich) and l-benzylpiperidin-4-amine (81.56 mmoles, 15.52 g, commercially available from Alfa-Aesar) in 100 ml of xylene was heated at reflux for 4 hours. Water (1.5 ml) was removed from the system via a Dean- Stark trap. The reaction was allowed to cool to 100 C and the resulting solid was filtered off. The recovered solid was washed with warm xylene and diethyl ether to give 18.9 g of l-(l-benzylpiperidin-4-yl)-5-oxopyrrolidine-3-carboxylic acid.. 1H NMR (400 MHz, d6-DMSO): 12.16 (br s, IH), 7.38 (m, 2H), 7.31 (m, IH), 7.27 (m, 2H), 3.78 (m, IH), 3.89 (s, 2H), 3.52 (m, IH), 3.77 (m, 2H), 3.36 (m, IH), 2.54 (m, IH), 2.43 (m,lH), 1.91 (m, 2H), 1.63 (m, 2H). MS (EI) for Cn H22 N2 O3: (MH+). 1 -(I -(tert-Butoxycarbonyl)piperidin-4-yl)-5-oxopyrrolidine-3-carboxylic acid
Figure imgf000332_0001
[00752] 1 -(I -Benzylpiperidin-4-yl)-5-oxopyrrolidine-3-carboxylic acid (33.11 mmoles, 10 g) was suspended in 100 ml of anhydrous methanol. Boc anhydride (41.22 mmoles, 9.0 g) and 5% palladium on charcoal (0.5 g, 50% w/w water) were added. The resulting mixture was hydrogenated at 1 atmosphere of hydrogen on a Parr hydrogenator until hydrogen uptake ceased. The solution was the filtered through a Celite plug. The plug was washed with additional methanol (2 x 20 ml) and the resulting filtrate was evaporated under reduced pressure to give an off-white solid. The crude product was subsequently recrystallized from ethano I/water to yield 7.8 g of l-(l-(tert-butoxycarbonyl)piperidin-4-yl)-5-oxopyrrolidine-3-carboxylic acid as a white solid. 1H NMR (400 MHz, d6-DMSO): 12.67 (br s, IH), 3.99 (br s, 2H), 3.89 (m, IH), 3.51 (t, ). MS (EI) for C15 H24N2 O5: 313 (MH+).
1,1-Dimethylethyl 4-(4-(8-bromo-4-oxo-3,4-dihvdrobenzofuror3,2-dlpyrimidin-2-yl)- 2-oxopyrrolidin- 1 -yDpiperidine- 1 -carboxylate [00753] l,l-Dimethylethyl 4-(4-(8-bromo-4-oxo-3,4-dihydrobenzofuro[3,2- d]pyrimidin-2-yl)-2-oxopyrrolidin- 1 -yl)piperidine- 1 -carboxylate was synthesized in a manner similar to 1,1-dimethylethyl 3-(5-bromo-2-carbamoylbenzofuran-3- ylcarbamoyl)azetidine- 1 -carboxylate wherein 1 -( 1 -(tert-butoxycarbonyl)piperidin-4- yl)-5-oxopyrrolidine-3-carboxylic acid replaced Boc-3-azetidine carboxylic acid. The crude material from this reaction was submitted to the next step without any further purification. MS (EI) for C24 H29 Br N4 O6: 550 (MH+). 1 , 1 -Dimethylethyl 4-(4-(5 -bromo-2-carbamoylbenzo furan-3-ylcarbamoyl)-2- oxopyrrolidin- 1 -yDpiperidine- 1 -carboxylate
[00754] 1 , 1 -Dimethylethyl 4-(4-(5 -bromo-2-carbamoylbenzo furan-3-ylcarbamoyl)- 2-oxopyrrolidin-l-yl)piperidine-l -carboxylate was synthesized in a similar manner as to 1,1 -dimethylethyl 4-(8-bromo-4-oxo-3,4-dihydrobenzofuro[3,2-d]pyrimidin-2- yl)piperidine- 1 -carboxylate, wherein 1 , 1 -dimethylethyl 4-(4-(8-bromo-4-oxo-3 ,4- dihydrobenzofuro [3 ,2-d]pyrimidin-2-yl)-2-oxopyrrolidin- 1 -yl)piperidine- 1 - carboxylate was substituted with 1,1 -dimethylethyl 4-(5-bromo-2- carbamoylbenzofuran-3-ylcarbamoyl)piperidine-l -carboxylate. The crude material from this reaction was used in the subsequent step without any further purification. MS (EI) for C24H27BrN4O5: 532 (MH+).
(Compound 304)
8-Bromo-2-(5-oxo-l-piperidin-4-ylpyrrolidin-3-yl)πibenzofuror3,2-dlpyrimidin- 4(3HVone [00755] 8-Bromo-2-(5-oxo-l-piperidin-4-ylpyrrolidin-3-yl)[l]benzofuro[3,2- d]pyrimidin-4(3H)-one as its hydrochloride salt, was synthesized in a similar manner to 8-bromo-2-piperidin-4-yl[l]benzofuro[3,2-d]pyrimidin-4(3H)-one (Compound
157.
1H NMR (400 MHz, d6-DMSO): 13.08 (br s, IH), 8.79 (br s, IH), 8.61 (br s, IH), 8.15 (d, IH), 7.84 (m, 2H), 4.11 (m, IH), 3.70 (m, 3H), 3.30 (m, 2H), 3.01 (m, 2H), 2.75 (d, 2H), 1.93 (m, 2H), 1.77 (m, 2H). MS (EI) for Ci9 Hi9 Br N4 O3: 432 (MH+).
(Compound 349)
1 , 1 -Dimethylethyl 4-(5 -bromo-2-carbamoylbenzo furan-3- ylcarbamovDbenzylcarbamate [00756] The intermediate 1, 1 -Dimethylethyl 4-(5 -bromo-2-carbamoylbenzo furan- 3-ylcarbamoyl)benzylcarbamate was synthesized in a manner similar to 1,1- dimethylethyl 3 -(5 -bromo-2-carbamoylbenzo furan-3 -ylcarbamoyl)azetidine- 1 - carboxylate wherein 4-((tert-butoxycarbonylamino)methyl)-benzoic acid (commercially available from CNH Technologies, Inc.) replaced Boc-3-azetidine carboxylic acid. The crude material from this reaction was submitted to the next step without any further purification. MS (EI) for C22 H22 Br N3 O5: 489 (MH+). 1,1-Dimethylethyl 4-(8-bromo-4-oxo-3,4-dihydrobenzofuror3,2-dlpyrimidin-2- vDbenzylcarbamate [00757] l,l-Dimethylethyl 4-(8-bromo-4-oxo-3,4-dihydrobenzofuro[3,2- d]pyrimidin-2-yl)benzylcarbamate was synthesized in a similar manner as to 1,1- dimethylethyl 4-(8-bromo-4-oxo-3,4-dihydrobenzofuro[3,2-d]pyrimidin-2- yl)piperidine- 1 -carboxylate wherein 1 , 1 -dimethylethyl 4-(5 -bromo-2- carbamoylbenzofuran-S-ylcarbamoyl) benzylcarbamate replaced 1 , 1 -dimethylethyl 4- (5-bromo-2-carbamoyl-benzofuran-3-ylcarbamoyl)piperidine-l -carboxylate. The resulting crude material from this reaction was used in the subsequent step without any further purification. MS (EI) for C22 H20 Br N3 O4: 471 (MH+). 2-[4-(Aminomethyl)phenyll-8-bromo[llbenzofuro[3,2-dlpyrimidin-4(3H)-one [00758] 2-[4-(Aminomethyl)phenyl]-8-bromo[l]benzofuro[3,2-d]pyrimidin-4(3H)- one as its hydrochloride salt, was synthesized in a similar manner to 8-bromo-2- piperidin-4-yl[l]benzofuro[3,2-d]pyrimidin-4(3H)-one (Compound 157), wherein 1,1 -dimethylethyl 4-(8-bromo-4-oxo-3,4-dihydrobenzofuro[3,2-d]pyrimidin-2- yl)piperidine-l -carboxylate was substituted with 1,1 -dimethylethyl 4-(8-bromo-4- oxo-3,4-dihydrobenzofuro[3,2-d]pyrimidin-2-yl)benzylcarbamate. 1H NMR (400 MHz, de-DMSO): 13.27 (br s, IH), 8.58 (br s, 3H), 8.28 (s, IH), 8.21 (d, 2H), 7.86 (m, 2H), 7.69 (d, 2H), 4.14 (s, 2H). MS (EI) for C17 H12 Br N3 O2: 371 (MH+).
(Compound 350)
1 , 1 -Dimethylethyl 3 -(2-(5 -bromo-2-carbamoylbenzofuran-3 -ylamino)- 2-oxoethyl)-4- oxo- 1 -phenyl- 1 ,3,8-triazaspiro|"4.51decane-8-carboxylate
[00759] 1,1 -Dimethylethyl 3-(2-(5-bromo-2-carbamoylbenzofuran-3-ylamino)-2- oxoethyl)-4-oxo- 1 -phenyl- 1, 3, 8-triazaspiro[4.5]decane-8-carboxylate was synthesized in a manner similar to 1,1 -dimethylethyl 3-(5-bromo-2-carbamoylbenzofuran-3- ylcarbamoyl)azetidine- 1 -carboxylate wherein 2-(8-(tert-butoxycarbonyl)-4-oxo- 1 - phenyl-l,3,8-triazaspiro[4.5]decan-3-yl)acetic acid (commercially available from NeoMPS group SNPE) replaced Boc-3-azetidine carboxylic acid. The crude material from this reaction was submitted to the next step without any further purification. MS (EI) for C29 H32 Br N5O6: 627 (MH+). 1 , 1 -Dimethylethyl 3 -((8-bromo-4-oxo-3 ,4-dihydrobenzofuro [3 ,2-dlpyrimidin-2- yl)methyl)-4-oxo-l-phenyl-l,3,8-triazaspiro[4.51decane-8-carboxylate
[00760] 1,1 -Dimethylethyl 3 -((8-bromo-4-oxo-3 ,4-dihydrobenzofuro [3,2- d]pyrimidin-2-yl)methyl)-4-oxo-l -phenyl- 1, 3, 8-triazaspiro[4.5]decane-8-carboxylate was synthesized in a similar manner as to 1,1 -dimethylethyl 4-(8-bromo-4-oxo-3,4- dihydrobenzofuro [3 ,2-d]pyrimidin-2-yl)piperidine- 1 -carboxylate wherein 1,1- dimethylethyl 3-(2-(5-bromo-2-carbamoylbenzofuran-3-ylamino)-2-oxoethyl)-4-oxo- l-phenyl-l,3,8-triazaspiro[4.5]-decane-8-carboxylate replaced 1,1-dimethylethyl 4-(5- bromo-2-carbamoyl-benzofuran-3 -ylcarbamoyl)piperidine- 1 -carboxylate . The pure crude material from this reaction was used in the subsequent step without any further purification. 1H NMR (400 MHz, d6-DMSO): 13.21 (br s,lH), 8.02 (s, IH), 7.82 (m, 2H), 7.23 (m, 2H), 6.79 (m, 3H), 4.86 (s,2H), 4.65 (s, 2H), 3.90 (, 2H), 4.41 (m, IH), 2.45 (m, 2H), 1.83 (d, 2H), 1.46 (s, 9H). MS (EI) for C29 H30 Br N5 O5: 609 (MH+).
8-Bromo-2-((4-oxo-l-phenyl-l,3,8-triazaspiror4.51decan-3-yl)methyl)benzofuror3,2- dlpyrimidin-4(3H)-one (Compound 157)
[00761] 8-Bromo-2-((4-oxo-l-phenyl-l,3,8-triazaspiro[4.5]decan-3- yl)methyl)benzofuro[3,2-d]pyrimidin-4(3H)-one as its hydrochloride salt, was synthesized in a similar manner to 8-bromo-2-piperidin-4-yl[l]benzofuro[3,2- d]pyrimidin-4(3H)-one, wherein 1,1-dimethylethyl 4-(8-bromo-4-oxo-3,4- dihydrobenzofuro [3 ,2-d]pyrimidin-2-yl)piperidine- 1 -carboxylate was substituted with 1,1-dimethylethyl 3-((8-bromo-4-oxo-3,4-dihydrobenzofuro[3,2-d]pyrimidin-2- yl)methyl)-4-oxo-l-phenyl-l,3,8-triazaspiro[4.5]-decane-8-carboxylate. 1H NMR (400 MHz, d6-DMSO): 13.26 (br s, IH), 9.10 (br d, 2H), 8.07 (d, IH), 7.83 (q, 2H), 7.27 (dd, 2H), 7.05 (d, 2H), 6.84 (IH), 4.88 (s, 2H), 4.68 (s, 2H), 3.58 (m, 4H), 2.85 dt, 2H), 2.00 (d, 2H). MS (EI) for C24 H22 Br N5 O3: 509 (MH+).
(Compound 351)
Ethyl 3 -hydroxy- 1 -methyl- 1 H-pyrazole-5 -carboxylate
COOEt
MeNHNH2
COOEt Et°H
Figure imgf000335_0001
[00762] A solution of diethyl acetylene dicarboxylate (100 mmoles. 17.01 g, 16.05 ml, commercially available from Sigma- Aldrich) in anhydrous ethanol (100 ml) was added in drop wise fashion to a stirred solution of methyl hydrazine (100 mmoles, 4.607, 5.35 ml) and anhydrous ethanol (150 ml), at room temperature, over the course of 9 hours. Upon completion of addition, the reaction mixture was stirred at room temperature for a further 20 hours. The reaction mixture was concentrated under reduced pressure and the resulting residue was triturated with cold acetonitrile to give a white solid. The solid was filtered off, washed with diethyl ether and dried under reduced pressure to give 9.Og of ethyl 3 -hydroxy- 1 -methyl- lH-pyrazole-5-carboxylate as a white solid. 1U NMR (400 MHz, d6-DMSO): 11.29 (br s, IH), 6.51 (s, IH), 4.32
(q, 2H), 4.12 (s, 3H), 1.43 (t, 3H). MS (EI) for C7 Hi0N2 O3: 171. (MH+).
1 , 1 -Dimethylethyl 4-(5 -(ethoxycarbonyl)- 1 -methyl- 1 H-pyrazol-3 -yloxy)piperidine- 1 - carboxylate
Figure imgf000336_0001
[00763] Anhydrous potassium carbonate (125 mmoles, 17.25 g) was added to a stirred solution of ethyl 3 -hydroxy- 1 -methyl- 1 H-pyrazole-5 -carboxylate (50 mmoles, 8.58 g,), 1,1-dimethylethyl 4-(methylsulfonyloxy)piperidine-l -carboxylate (50 mmoles, 17.25 g, commercially available from 3B Medical Systems, Inc.) in anhydrous anhydrous DMF (100 mL) at room temperature. The reaction mixture was then stirred at 110 0C for 8 hours. The reaction mixture was then allowed to cool to room temperature and diluted with cold water (500 ml). The mixture was then extracted with ethyl acetate (4 x 250ml). The combined ethyl acetate solution was then washed with water (3 x 200 ml), saturated sodium chloride solution (250 ml), dried over anhydrous sodium sulfate, filtered off and evaporated under reduced pressure. The crude material was then recrystallized from benzene (200 ml) to give 10.05 g of 1,1-dimethylethyl 4-(5 -(ethoxycarbony I)-I -methyl- lH-pyrazol-3 - yloxy)piperidine-l -carboxylate as an off- white solid. 1H NMR (400 MHz, d6- DMSO): 13.39 (br s, IH), 6.23 (s, IH), 4.57 (m, IH), 4.32 (q, 2H), 3.19 (s, 3H), 3.65 (m, 2H), 3.14 (m, 2H), 1.92 (d, 2H), 1.54 (t, 3H), 1.49 (d, 2H), 1.43 (t, 3H), 1.39 (d, 9H). MS (EI) for Cn H27N3 O5: 354 (MH+).
1 , 1 -dimethylethyl 4-(5 -(carboxy)- 1 -methyl- 1 H-pyrazol-3 -yloxy)piperidine- 1 - carboxylate
Figure imgf000336_0002
[00764] 1,1-dimethylethyl 4-(5-(ethoxycarbonyl)-l -methyl- 1 H-pyrazol-3 - yloxy)piperidine-l-carboxylate (28.29 mmole, 10 g) was dissolved in methanol (200 ml). Aqueous IM sodium hydroxide solution (43, 43 mmoles) was added and the reaction mixture was stirred at room temperature for 16 hours. The reaction mixture was cooled to 5 oC (ice-water batch) and acidified to pH 1 with aqueous IM hydrochloric acid. The resulting white solid was filtered off, washed with cold water (2 x 20 ml) and dried under reduced pressure to give 9.18 g of 1,1-dimethylethyl 4- (5 -(carboxy)-l -methyl- lH-pyrazol-3-yloxy)piperidine-l-carboxylate as a white solid. 1H NMR (400 MHz, d6-DMSO):, 13.39 (br s, IH), 6.23 (s, IH), 4.57 (m, IH), 4.32 (q, 2H), 3.19 (s, 3H), 3.65 (m, 2H), 3.14 (m, 2H), 1.92 (d, 2H), 1.54 (t, 3H), 1.49 (d, 2H), 1.39 (d, 9H). MS (EI) for Ci5H23N3O5: 326 (MH+).
1 , 1 -dimethylethyl 4-(5-(5-bromo-2-carbamoylbenzofuran-3-ylcarbamoyl)- 1 -methyl- lH-pyrazol-3-yloxy)piperidine-l-carboxylate [00765] 1 , 1 -Dimethylethyl 4-(5-(5-bromo-2-carbamoylbenzofuran-3-ylcarbamoyl)- 1 -methyl- 1 H-pyrazol-3 -yloxy)piperidine- 1 -carboxylate was synthesized in a manner similar to 1,1-dimethylethyl 3-(5-bromo-2-carbamoylbenzofuran-3- ylcarbamoyl)azetidine- 1 -carboxylate wherein 1 , 1 -dimethylethyl 4-(5 -(carboxy)- 1 - methyl- 1 H-pyrazol-3 -yloxy)piperidine-l -carboxylate was substituted with Boc-3- azetidine carboxylic acid. The crude material from this reaction was submitted to the next step without any further purification. MS (EI) for C24 H28 Br N5 O6: 563 (MH+). 1,1-dimethylethyl 4-(5-(8-bromo-4-oxo-3,4-dihvdrobenzofuror3,2-dlpyrimidin-2-yl)- 1 -methyl- 1 H-pyrazol-3 -yloxy)piperidine- 1 -carboxylate
[00766] l,l-Dimethylethyl 4-(5-(8-bromo-4-oxo-3,4-dihydrobenzofuro[3,2- d]pyrimidin-2-yl)- 1 -methyl- 1 H-pyrazol-3 -yloxy)piperidine- 1 -carboxylate was synthesized in a similar manner as to 1,1-dimethylethyl 4-(8-bromo-4-oxo-3,4- dihydrobenzofuro [3 ,2-d]pyrimidin-2-yl)piperidine- 1 -carboxylate wherein 1,1- dimethylethyl 4-(5-(5 -bromo-2-carbamoylbenzofuran-3 -ylcarbamoyl)- 1 -methyl- IH- pyrazol-3-yloxy)piperidine-l -carboxylate was substituted with 1,1-dimethylethyl 4- (5-bromo-2-carbamoylbenzofuran-3-ylcarbamoyl)piperidine- 1 -carboxylate. The crude material from this reaction was used in the subsequent step without any further purification. MS (EI) for C24 H26 Br N5 O5: 545 (MH+). 8-Bromo-2-[l-methyl-3-(piperidin-4-yloxy)-lH-pyrazol-5-yll[llbenzofuro[3,2- dlpyrimidin-4(3H)-one (Compound 351)
[00767] 8-Bromo-2-[l-methyl-3-(piperidin-4-yloxy)-lH-pyrazol-5- yl][l]benzofuro[3,2-d]pyrimidin-4(3H)-one as its hydrochloride salt, was synthesized in a similar manner to 8-bromo-2-piperidin-4-yl[l]benzofuro[3,2-d]pyrimidin-4(3H)- one (Compound 157) wherein 1,1-dimethylethyl 4-(8-bromo-4-oxo-3,4- dihydrobenzofuro[3,2-d]pyrimidin-2-yl)piperidine-l-carboxylate was substituted with
1,1-dimethylethyl 4-(5-(8-bromo-4-oxo-3,4-dihydrobenzofuro[3,2-d]pyrimidin-2-yl)- l-methyl-lH-pyrazol-3-yloxy)piperidine-l-carboxylate. 1H NMR (400 MHz, d6- DMSO): 13.14 (br s, IH), 9.00 (br s, 2H), 8.31 (d, IH), 7.60 (m, 2H), 6.5 (s, IH), 4.71
(m, IH), 4.12 (s, 3H), 3.22 (m, 2H), 3.08 (m, 2H), 2.17 (m, 2H), 1.90 (m, 2H). MS
(EI) for Ci9 Hi8 Br N5 O3 : 445 (MH+).
(Compound 352)
1 , 1 -Dimethylethyl (4-(5-bromo-2-carbamoylbenzofuran-3-ylcarbamoyl)thiazol-2- vDmethylcarbamate
[00768] 1,1 -Dimethylethyl (4-(5-bromo-2-carbamoylbenzofuran-3- ylcarbamoyl)thiazol-2-yl)methylcarbamate was synthesized in a manner similar to 1 , 1 -dimethylethyl 3 -(5 -bromo-2-carbamoylbenzofuran-3 -ylcarbamoyl)azetidine- 1 - carboxylate wherein 2-((tert-butoxycarbonylamino)methyl)thiazole-4-carboxylic acid (prepared by a literature method Tetrahedron (1986), 42: 10, 2695) replaced Boc-3- azetidine carboxylic acid. The crude material from this reaction was submitted to the next step without any further purification.. MS (EI) for C19 H19 Br N4 O5 S: 496 (MH+). 1,1 -Dimethylethyl (4-(8-bromo-4-oxo-3,4-dihydrobenzomro[3,2-d]pyrimidin-2- yl)thiazol-2-yl)methylcarbamate
[00769] l,l-Dimethylethyl (4-(8-bromo-4-oxo-3,4-dihydrobenzofuro[3,2- d]pyrimidin-2-yl)thiazol-2-yl)methylcarbamate was synthesized in a similar manner as to 1,1-dimethylethyl 4-(8-bromo-4-oxo-3,4-dihydrobenzofuro[3,2-d]pyrimidin-2- yl)piperidine-l -carboxylate, wherein 1,1-dimethylethyl (4-(5-bromo-2- carbamoylbenzofuran-3 -ylcarbamoyl)thiazol-2-yl)methylcarbamate replaced 1,1- dimethylethyl 4-(5-bromo-2-carbamoylbenzofuran-3-ylcarbamoyl)-piperidine- 1 - carboxylate. The crude material from this reaction was used in the subsequent step without any further purification. MS (EI) for Ci9 Hn Br N4 O4S: 478 (MH+). 2-r2-(Aminomethvπ-1.3-thiazol-4-yl1-8-bromoπibenzofuror3.2-d1pyrimidin-4(3H)- one
[00770] 2-[2-(Aminomethyl)-l,3-thiazol-4-yl]-8-bromo[l]benzofuro[3,2- d]pyrimidin-4(3H)-one (Compound 352) as its hydrochloride salt, was synthesized in a similar manner to 8-bromo-2-piperidin-4-yl[l]benzofuro[3,2-d]pyrimidin-4(3H)- one (Compound 157) wherein 1,1-dimethylethyl 4-(8-bromo-4-oxo-3,4- dihydrobenzofuro[3,2-d]pyrimidin-2-yl)piperidine-l-carboxylate was substituted with 1,1-dimethylethyl (4-(8-bromo-4-oxo-3,4-dihydrobenzofuro[3,2-d]pyrimidin-2- yl)thiazol-2-yl)methylcarbamate. 1U NMR (400 MHz, d6-DMSO): 12.71 (br s, IH), 8.66 (s, IH), 8.23 (d, IH), 7.95 (m, 2H), 4.66 (d, 2H), 3.5 (br s , 3H). MS (EI) for Ci4 H9 Br N4 O2 S: 378 (MH+).
(Compound 353)
8-Bromo-2-{3-[(dimethylamino)methyllphenyl|[llbenzofuro[3,2-dlpyrimidin-4(3H)- one [00771] 8-Bromo-2- {3-[(dimethylamino)methyl]phenyl} [ 1 ]benzofuro[3,2- d]pyrimidin-4(3H)-one as its acetate salt was synthesized in a manner similar to 8- bromo-2-(l-methylpiperidin-3-yl)[l]benzofuro[3,2-d]pyrimidin-4(3H)-one, wherein 2- [3 -(Aminomethyl)phenyl] -8-bromo [ 1 ]benzofuro [3 ,2-d]pyrimidin-4(3H)-one (Compound 302) replaced 8-bromo-2-piperidin-3-yl[l]benzofuro[3,2-d]pyrimidin- 4(3H)-one dihydrochloride (Compound 184). 1H NMR (400 MHz, d6-DMSO): 12.04 (br s, IH), 8.19 (s, IH), 8.11 (s, 2H), 7.83 (s, 2H), 7.57 (d, 2H), 3.85 (s, 2H), 3.44 (br s, 3H), 2.43 (s, 6H)._MS (EI) for Ci9 Hj6 Br N3 O2 : 399 (MH+).
(Compound 354)
1 , 1 -Dimethylethyl 4-(5-bromo-2-carbamoylbenzofuran-3- ylcarbamovDphenylcarbamate
[00772] 1 , 1 -Dimethylethyl 4-(5-bromo-2-carbamoylbenzofuran-3- ylcarbamoyl)phenylcarbamate was synthesized in a manner similar to 1,1- dimethylethyl 3 -(5 -bromo-2-carbamoylbenzofuran-3 -ylcarbamoyl)azetidine- 1 - carboxylate wherein 4-(tert-butoxycarbonylamino)benzoic acid (commercially available from CNH Technologies Inc.,) replaced Boc-3-azetidine carboxylic acid. The crude material from this reaction was submitted to the next step without any further purification. MS (EI) for C21 H20 Br N305: 475 (MH+). 1,1 -Dimethylethyl 4-(8-bromo-4-oxo-3,4-dihydrobenzofuro[3,2-d]pyrimidin-2- yPphenylcarbamate
[00773] l,l-Dimethylethyl 4-(8-bromo-4-oxo-3,4-dihydrobenzofuro[3,2- d]pyrimidin-2-yl)phenylcarbamate was synthesized in a similar manner as to 1,1- dimethylethyl 4-(8-bromo-4-oxo-3,4-dihydrobenzofuro[3,2-d]pyrimidin-2- yl)piperidine- 1 -carboxylate wherein 1 , 1 -dimethylethyl 4-(5 -bromo-2- carbamoylbenzofuran-3-ylcarbamoyl)phenylcarbamate replaced 1,1 -dimethylethyl 4- (5-bromo-2-carbamoylbenzofuran-3-ylcarbamoyl)piperidine- 1 -carboxylate and heated at 100 0C for 16 hours. The crude material from this reaction was used in the subsequent step without any further purification. MS (EI) for C21 Hig Br N3 O4: 457 (MH+).
2-(4-Aminophenyl)-8-bromoπibenzofuror3,2-dlpyrimidin-4(3H)-one (Compound 354)
[00774] 2-(4-Aminophenyl)-8-bromo[l]benzofuro[3,2-d]pyrimidin-4(3H)-one as its hydrochloride salt, was synthesized in a similar manner to 8-bromo-2-piperidin-4- yl[l]benzofuro[3,2-d]pyrimidin-4(3H)-one (Compound 157), wherein 1,1- dimethylethyl 4-(8-bromo-4-oxo-3,4-dihydrobenzofuro[3,2-d]pyrimidin-2- yl)piperidine-l -carboxylate was substituted with 1,1 -dimethylethyl 4-(8-bromo-4- oxo-3,4-dihydrobenzofuro[3,2-d]pyrimidin-2-yl)phenylcarbamate 1H NMR (400 MHz, de-DMSO): 12.94 (br s, IH), 8.25 (m, IH), 8.07 (d, 2H), 7.83 (m, 2H). 6.99 (d,
2H), 4.78 (br s, 3H). MS (EI) for Ci6 Hi0 Br N3 O2: 357 (MH+).
(Compound 355)
1 , 1 -Dimethylethyl 3-(5-bromo-2-carbamoylbenzofuran-3- ylcarbamovDphenylcarbamate [00775] 1, 1 -Dimethylethyl 4-(5-bromo-2-carbamoylbenzofuran-3- ylcarbamoyl)phenylcarbamate was synthesized in a manner similar to 1,1- dimethylethyl 3 -(5 -bromo-2-carbamoylbenzofuran-3 -ylcarbamoyl)azetidine- 1 - carboxylate 3-(tert-butoxycarbonylamino)benzoic acid (commercially available from CNH Technologies Inc.,) replaced Boc-3-azetidine carboxylic acid. The crude material from this reaction was submitted to the next step without any further purification. MS (EI) for C2IH20BrN3O5: 475 (MH+). 1 , 1 -Dimethylethyl 3 -(8-bromo-4-oxo-3 ,4-dihvdrobenzofuro [3 ,2-d1pyrimidin-2- vDphenylcarbamate [00776] l,l-Dimethylethyl 3-(8-bromo-4-oxo-3,4-dihydrobenzofuro[3,2- d]pyrimidin-2-yl)phenylcarbamate was synthesized in a similar manner as to 1,1- dimethylethyl 3-(8-bromo-4-oxo-3,4-dihydrobenzofuro[3,2-d]pyrimidin-2- yl)piperidine- 1 -carboxylate, wherein 1 , 1 -dimethylethyl 4-(5 -bromo-2- carbamoylbenzofuran-3 -ylcarbamoyl)phenylcarbamate replaced 1 , 1 -dimethylethyl 4- (5-bromo-2-carbamoylbenzofuran-3-ylcarbamoyl)piperidine- 1 -carboxylate and heated at 100 0C for 16 hours. The crude material from this reaction was used in the subsequent step without any further purification. MS (EI) for C2i Hi8 Br N3 O4: 457 (MH+). 2-(3-Aminophenyl)-8-bromo[llbenzofuro[3,2-dlpyrimidin-4(3H)-one (Compound 355) [00777] 2-(3-Aminophenyl)-8-bromo[l]benzofuro[3,2-d]pyrimidin-4(3H)-one as its hydrochloride salt, was synthesized in a similar manner to 8-bromo-2-piperidin- 4-yl[l]benzofuro[3,2-d]pyrimidin-4(3H)-one (Compound 157) wherein 1,1- dimethylethyl 3-(8-bromo-4-oxo-3,4-dihydrobenzofuro[3,2-d]pyrimidin-2- yl)piperidine-l -carboxylate was substituted with 1,1 -dimethylethyl 4-(8-bromo-4- oxo-3,4-dihydrobenzofuro[3,2-d]pyrimidin-2-yl)phenylcarbamate. 1H NMR (400 MHz, de-DMSO): 13.02 (br s, IH), 8.26 (s, IH), 7.84 (d, 2H), 7.47 (m, IH), 7.23 (d, IH), 7.03 (m,lH), 6.89 (d, IH). 6.43 (br s, 3H). MS (EI) for Ci6 HJ0 Br N3 O2: 357 (MH+).
(Compound 358)
8-Bromo-2-{4-[(dimethylamino)methyllphenyl|[llbenzofuro[3,2-dlpyrimidin-4(3H)- one
[00778] 8-Bromo-2-{4-[(dimethylamino)methyl]phenyl}[l]benzofuro[3,2- d]pyrimidin-4(3H)-one as its acetate salt was synthesized in a manner similar to 8- bromo-2-(l -methylpiperidin-3-yl)[ 1 ]benzofuro[3,2-d]pyrimidin-4(3H)-one wherein 2- [4-(aminomethyl)phenyl]-8-bromo[l]benzofuro[3,2-d]pyrimidin-4(3H)-one replaced 8-bromo-2-piperidin-3-yl[l]benzofuro[3,2-d]pyrimidin-4(3H)-one dihydrochloride (Compound 184). 1H NMR (400 MHz, d6-DMSO): 12.96 (br s, IH), 8.26 (s, IH), 8.15 (d, 2H), 7.84 (s, 2H), 7.48 (d, 2H), 3.51 (s, 2H), 3.35 (br s, 2H), 2.20 (s, 6H). MS (EI) for Ci9 Hi6 Br N3 O2: 399 (MH+).
(Compound 359)
1 , 1 -Dimethylethyl 2-((4-(methoxycarbonyl)phenoxy)methyl)morpholine-4- carboxylate
Figure imgf000342_0001
[00779] Cesium carbonate (37.5 mmoles, 12.21 g) was added in one lot to a stirred solution of methyl 4-hydroxybenzoate (25 mmole 3.80 g) and 1,1 -dimethylethyl 2- (chloromethyl) morpholine-4-carboxylate (25 mmoles 7.36 g), (commercially available from Butt Park Ltd.) in anhydrous DMF. The reaction mixture was then stirred at 80 0C for 16 hours. The reaction mixture was then allowed to cool to room temperature, quenched with ice-water and washed with ethyl acetate (4 x 200 ml). The combined organic solution was washed with water (2 x 200 ml), saturated brine (200 ml), dried over anhydrous magnesium chloride, filtered and evaporated under reduced pressure to give 9.02 g of 1,1 -dimethylethyl 2-((4- (methoxycarbonyl)phenoxy)methyl) morpholine-4-carboxylate as a white solid, which was used in the nest step without any further purification. 1H NMR (400 MHz, de-DMSO): 1H NMR (400 MHz, d6-DMSO): 7.79 (d, 2H), 7.01 (d, 2H), 4.26 (s, 3H), 4.06 (m, 2H), 3.87 (m, 2H), 3.68 (m, 2H), 3.50 (m, IH), 3.19 (m, 2H), 2.89 (m, 2H), 1.41 (s, IH). MS (EI) for Ci8 H25N O6 : 352 (MH+). 4-((4-( 1 , 1 -Dimethylethoxycarbonyl)morpholin-2-yl)methoxy)benzoic acid
Figure imgf000342_0002
[00780] Crude 1,1-dimethylethyl 2-((4-(methoxycarbonyl)phenoxy)methyl)- morpholine-4-carboxylate (25 mmole, 8.77 g) was dissolved in methanol (200 ml). Aqueous IM sodium hydroxide solution (50 mL, 50 mmoles) was added and the reaction mixture was stirred at room temperature for 36 hours. The reaction mixture was cooled to 5 oC (ice-water batch) and acidified to pH 1 with aqueous IM hydrochloric acid. The resulting white solid was filtered off, washed with cold water (2 x 20 ml) and dried under reduced pressure to give 8.21 g of 4-((4-(l,l- dimethylethoxycarbonyl)morpholin-2-yl)methoxy)benzoic acid as a off-white solid. 1H NMR (400 MHz, d6-DMSO): 12.61 (br s, IH), 7.78 (d, 2H), 7.03 (d, 2H), 4.06 (m, 2H), 3.87 (m, 2H), 3.68 (m, 2H), 3.50 (m, IH), 3.19 (m, 2H), 2.89 (m, 2H), 1.41 (s, IH). MS (EI) for Cn H23N O6: 338 (MH+).
1 , 1 -Dimethylethyl 2-((4-(5-bromo-2-carbamoylbenzofuran-3-ylcarbamoyl)phenoxy)- methyl)morpholine-4-carboxylate [00781] 1 , 1 -Dimethylethyl 2-((4-(5-bromo-2-carbamoylbenzofuran-3- ylcarbamoyl)phenoxy)-methyl) morpholine-4-carboxylate was synthesized in a manner similar to 1,1-dimethylethyl 3-(5-bromo-2-carbamoylbenzofuran-3- ylcarbamoyl)azetidine-l-carboxylate, wherein 4-((4-(l,l- dimethylethoxycarbonyl)morpholin-2-yl)methoxy)benzoic acid replaced Boc-3- azetidine carboxylic acid. The crude material from this reaction was submitted to the next step without any further purification. MS (EI) for C26 H28 Br N3 O7: 575 (MH+). 1,1-Dimethylethyl 2-((4-(8-bromo-4-oxo-3,4-dihydrobenzofuror3,2-dlpyrimidin-2- yl)phenoxy)methyl)morpholine-4-carboxylate
[00782] l,l-Dimethylethyl 2-((4-(8-bromo-4-oxo-3,4-dihydrobenzofuro[3,2- d]pyrimidin-2-yl)phenoxy)methyl)morpholine-4-carboxylate was synthesized in a similar manner as to 1,1-dimethylethyl 4-(8-bromo-4-oxo-3,4-dihydrobenzofuro[3,2- d]pyrimidin-2-yl)piperidine-l-carboxylate, wherein 1,1-dimethylethyl 2-((4-(5- bromo-2-carbamoylbenzofuran-3-ylcarbamoyl)phenoxy)methyl)morpholine-4- carboxylate replaced 1,1-dimethylethyl 4-(5-bromo-2-carbamoylbenzofuran-3- ylcarbamoyl)piperidine-l-carboxylate. The crude material from this reaction was used in the subsequent step without any further purification. MS (EI) for C26 H26 Br N3 O6: 557 (MH+).
8-Bromo-2-{4-[(moipholin-2-ylmethvπoxylphenyl}[llbenzofuro[3,2-dlpyrimidin- 4(3HVone [00783] 8-Bromo-2-{4-[(morpholin-2-ylmethyl)oxy]phenyl}[l]benzofuro[3,2- d]pyrimidin-4(3H)-one (Compound 359) as its hydrochloride salt, was synthesized in a similar manner to 8-bromo-2-piperidin-4-yl[l]benzofuro[3,2-d]pyrimidin-4(3H)- one (Compound 157), wherein 1,1-dimethylethyl 4-(8-bromo-4-oxo-3,4- dihydrobenzofuro[3,2-d]pyrimidin-2-yl)piperidine-l-carboxylate was substituted with 1,1-dimethylethyl 2-((4-(8-bromo-4-oxo-3,4-dihydrobenzofuro[3,2-d]pyrimidin-2- yl)phenoxy)methyl)morpholine-4-carboxylate
1H NMR (400 MHz, d6-DMSO): 12.79 (br s, IH), 8.26 (s, IH), 8.18 (d, 2H), 7.84 (s, 2H), 7.13 (d, 2H), 4.18 (m, 3H),4.02 (dd, IH), 3.85 (t, IH), 3.37 (d, IH), 2.31 (d, IH), 2.99 (m, 2H). MS (EI) for C21 Hi8 Br N3 O4: 457 (MH+).
(Compound 375)
1 , 1 -Dimethylethyl 5 -(5 -bromo-2-carbamoylbenzofuran-3 -ylcarbamoyl)-3 A- dihvdroisoquinoline-2( 1 H)-carboxylate [00784] 1 , 1 -Dimethylethyl 5 -(5 -bromo-2-carbamoylbenzofuran-3 -ylcarbamoyl)- 3,4-dihydroisoquinoline-2(lH)-carboxylate was synthesized in a manner similar to 1 , 1 -dimethylethyl 3 -(5 -bromo-2-carbamoylbenzofuran-3 -ylcarbamoyl)azetidine- 1 - carboxylate wherein 2-(tert-butoxycarbonyl)-l ,2,3,4-tetrahydroisoquinoline-5- carboxylic acid (commercially available from ASW MedChem, Inc.) replaced Boc-3- azetidine carboxylic acid. The crude material from this reaction was submitted to the next step without any further purification. MS (EI) for C24 H24 Br N3 O5 : 515 (MH+). 1 , 1 -Dimethylethyl 5 -(8-bromo-4-oxo-3 ,4-dihvdrobenzofuro [3 ,2-d1pyrimidin-2-yl)- 3,4-dihvdroisoquinoline-2(lH)-carboxylate
[00785] l,l-Dimethylethyl 5-(8-bromo-4-oxo-3,4-dihydrobenzofuro[3,2- d]pyrimidin-2-yl)-3,4-dihydroisoquinoline-2(lH)-carboxylate was synthesized in a similar manner as to 1,1-dimethylethyl 4-(8-bromo-4-oxo-3,4-dihydrobenzofuro[3,2- d]pyrimidin-2-yl)piperidine-l -carboxylate, wherein 1,1-dimethylethyl 5-(5-bromo-2- carbamoylbenzofuran-3 -y lcarbamoyl)-3 ,4-dihydroisoquinoline-2( 1 H)-carboxylate replaced 1,1-dimethylethyl 4-(5-bromo-2-carbamoylbenzofuran-3- ylcarbamoyl)piperidine-l -carboxylate. The crude material from this reaction was used in the subsequent step without any further purification. 1H NMR (400 MHz, d6- DMSO): 12.94 (br s, IH), 8.24 (s, IH), 7.85 (m, 2H), 7.43 (d, IH), 7.38 (m, 2H), 4.60 (s, 2H), 3.52 (s, 2H), 2.86 (s, 2H), 1.44 (s, 9H). MS (EI) for C24 H22 Br N3 O4: 467 (MH+). 8-Bromo-2-(l,2,3,4-tetrahydroisoquinolin-5-yl)[llbenzofuro[3,2-dlpyrimidin-4(3H)- one
[00786] 8-Bromo-2-(l,2,3,4-tetrahydroisoquinolin-5-yl)[l]benzofuro[3,2- d]pyrimidin-4(3H)-one (Compound 375) as its hydrochloride salt, was synthesized in a similar manner to 8-bromo-2-piperidin-4-yl[l]benzofuro[3,2-d]pyrimidin-4(3H)-one (Compound 157), wherein 1,1-dimethylethyl 4-(8-bromo-4-oxo-3,4- dihydrobenzofuro[3,2-d]pyrimidin-2-yl)piperidine-l-carboxylate was substituted with 1,1-dimethylethyl 5-(8-bromo-4-oxo-3,4-dihydrobenzofuro[3,2-d]pyrimidin-2-yl)- 3,4-dihydroisoquinoline-2(lH)-carboxylate. 1H NMR (400 MHz, d6-DMSO): 13.02 (br s, IH), 9.91 (br s, 2H), 8.18 (d, IH), 7.87 (q, 2H), 6.54 (dd, IH), 7.44 (s, 2H), 4.36 (s, 2H), 3.31 (s, 2H), 3.15 (m, 2H). MS (EI) for Ci9 H14 Br N3 O2: 397 (MH+).
(Compound 376)
2-rtrα/?5-4-(Aminomethyl)cvclohexyll-8-bromorilbenzofuror3,2-dlpyrimidin-4(3H)- one
(trα/?5)-4-((tert-butoxycarbonylamino)methyl)cyclohexanecarboxylic acid [00787] 1 , 1 -Dimethylethyl (£rαns-4-(5-bromo-2-carbamoylbenzofuran-3- ylcarbamoyl)cyclohexyl) methylcarbamate was synthesized in a manner similar to 1 , 1 -dimethylethyl 3 -(5 -bromo-2-carbamoylbenzofuran-3 -ylcarbamoyl)azetidine- 1 - carboxylate wherein (£rans)-4-((tert-butoxycarbonylamino)methyl)cyclohexane- carboxylic acid (commercially available from NeoMPS, Groupe SNPE) replaced Boc- 3-azetidine carboxylic acid. The crude material from this reaction was submitted to the next step without any further purification. MS (EI) for C22 H28 Br N3 O5: 495 (MH+). 1,1 -Dimethylethyl (frαfts-4-(8-bromo-4-oxo-3,4-dihydrobenzofuro[3,2-d]pyrimidin-2- vDcvclohexyDmethylcarbamate
[00788] l,l-Dimethylethyl (trα/?5-4-(8-bromo-4-oxo-3,4-dihydrobenzofuro[3,2- d]pyrimidin-2-yl)cyclohexyl)methylcarbamate was synthesized in a similar manner as to 1,1-dimethylethyl 4-(8-bromo-4-oxo-3,4-dihydrobenzofuro[3,2-d]pyrimidin-2- yl)piperidine-l -carboxylate, wherein 1,1-dimethylethyl ((lr,4r)-4-(5-bromo-2- carbamoylbenzofuran-3-ylcarbamoyl)cyclohexyl)methylcarbamate replaced 1,1- dimethylethyl 4-(5 -bromo-2-carbamoylbenzofuran-3 -ylcarbamoyl)piperidine- 1 - carboxylate. The crude material from this reaction was used in the subsequent step without any further purification. 1H NMR (400 MHz, d6-DMSO): 12.80 (br s, IH), 8.15 (s, IH), 7.81 (s, 2H), 6.88 (t, IH), 3.35 (s, 2H), 2.87 (t, 2H), 2.64 (t, IH), 1.96 (d, 2H), 1.79 (d, 2H), 1.61 (m, IH), 1.39 (s, 9H), 0.97 (m, IH). MS (EI) for C22 H26 Br N3 O4: 477 (MH+). 2-[trα/?5-4-(Aminomethyl)cyclohexyll-8-bromo[llbenzofuro[3,2-dlpyrimidin-4(3H)- one
[00789] 2-[trα/?5-4-(Aminomethyl)cyclohexyl]-8-bromo[l]benzofuro[3,2- d]pyrimidin-4(3H)-one (Compound 376) as its hydrochloride salt, was synthesized in a similar manner to 8-bromo-2-piperidin-4-yl[l]benzofuro[3,2-d]pyrimidin-4(3H)-one (Compound 157), wherein 1,1-dimethylethyl 4-(8-bromo-4-oxo-3,4- dihydrobenzofuro[3,2-d]pyrimidin-2-yl)piperidine-l-carboxylate was substituted with 1,1-dimethylethyl ((trαns)-4-(8-bromo-4-oxo-3,4-dihydrobenzofuro[3,2-d]pyrimidin- 2-yl)cyclohexyl)methylcarbamate. 1H NMR (400 MHz, d6-DMSO): 12.86 (br s, IH), 816 (s, IH), 8.02 (br s, 3H), 7.81 (s, 2H), 2.70 (m, 2H), 1.98 (m, 4H), 1.65 (m, 3H), 1.07 (m, 2H). MS (EI) for C17 Hi8 Br N3 O2: 377(MH+).
(Compound 377)
8-Bromo-2-(2-piperidin-4-ylpyrazolo[l,5-a]pyrimidin-6-yl)[l]benzofuro[3,2- d]pyrimidin-4(3H)-one
1 , 1 -Dimethylethyl 4-(5 -amino- 1 H-pyrazol-3 -vDpiperidine- 1 -carboxylate
Figure imgf000346_0001
[00790] Hydrazine hydrate (149.57 mmoles, 7.26 ml) was added to a stirred solution of tert-tutyl 4-(2-cyanoacetyl)piperidinecarboxylate (49.85 mmoles, 12.58 g, purchased from Butt Park, Ltd.) in absolute ethanol (60 ml) The stirred reaction mixture was then refluxed for 5 hours. The reaction mixture was allowed to cool to room temperature and concentrated under reduced pressure. The viscous oil was dissolved in ethyl acetate (200 ml) and washed with water (2 x 100 ml) and saturated brine (200 ML). The ethyl acetate solution was dried over anhydrous magnesium sulfate, filtered and evaporated under reduced pressure. The residue was treated with diethyl ether (300 ml) and stirred at 0-5 0C (ice-water bath). A white solid started to form after 20 minutes. The resulting slurry was then stirred at 0-5 0C (ice-water bath) for 3 hours. The solid was filtered off, washed with cold ether (2 x 50 ml) and hexane (100 ml) and dried at reduced pressure to give 12.04 g of 1,1-dimethylethyl 4-(5- amino-lH-pyrazol-3-yl)piperidine-l-carboxylate as a white solid. 1H NMR (400
MHz, de-DMSO): 12.71 (br s, 1), 6.34 (s, IH), 6.12 (br s, IH), 3.43 (m, 2H), 3.37 (m,
2H), 2.97 (m, IH), 2.04 (m, 2H), 1.81 (m, 2H), 1.42 (s, 9H). MS (EI) for Ci3 H22N4
O2: 267 (MH+).
Ethyl 2-(l-(tert-butoxycarbonyl)piperidin-4-yl)pyrazolo[l,5-alpyrimidine-6- carboxylate
Figure imgf000347_0001
[00791] Ethyl 2-formyl-3-oxopropanoate (51.04 mmoles, 7.35 g, prepared using a literature method, Synthesis (1986),5, 400.) was added as one lot to a stirred solution of 1,1-dimethylethyl 4-(5 -amino- lH-pyrazol-3-yl)piperidine-l-carboxylate (44.38 moles, 11.82 g), in anhydrous methanol (100 mL). The reaction mixture was then stirred at room temperature. After 30 minutes, a yellow precipitate was observed. After 60 minutes, the resulting solid was filtered off , washed with methanol (2 x 10 ml) and dried under reduced pressure to give 15.93 g of ethyl 2-(l-(tert- butoxycarbonyl)piperidin-4-yl)pyrazolo[l ,5-a]pyrimidine-6-carboxylate, which was used in the next step without any further purification. 1H NMR (400 MHz, de- DMSO): 8.42 (d, IH), 8.81 (d, IH), 6.78 (s, IH), 4.32 (q, 2H),4.03 (m, 2H), 3.03 (m, IH), 2.90 (m, 2H), 1.98 (d, 2H), 1.61 (m, 2H), 1.41 (s, 9H), 1.31 (t, 3H). MS (EI) for Ci9 H26N4 O4: 375: (MH+).
2-(l -(tert-Butoxycarbonyl)piperidin-4-yl)pyrazolo[ 1 ,5-a"|pyrimidine-6-carboxylic acid
Figure imgf000347_0002
[00792] Ethyl 2-(l-(tert-butoxycarbonyl)piperidin-4-yl)pyrazolo[l,5-a]pyrimidine- 6-carboxylate (25 mmole, 9.35 g) was dissolved in methanol (200 ml). Aqueous IM sodium hydroxide solution (50 mL, 50 mmoles) was added and the reaction mixture was stirred at room temperature for 16 hours. The reaction mixture was cooled to 5 oC (ice-water batch) and acidified to pH 1 with aqueous IM hydrochloric acid. The resulting white solid was filtered off, washed with cold water (2 x 20 ml) and dried under reduced pressure to give 8.41 g of 2-(l-(tert-butoxycarbonyl)piperidin-4- yl)pyrazolo[l,5-a]pyrimidine-6-carboxylic acid as a white solid. 1H NMR (400 MHz, de-DMSO): 13.56 (br s, IH), 8.41 (d, IH), 8.82 (d, IH), 6.78 (s, IH), 4.03 (m, 2H), 3.03 (m, IH), 2.90 (m, 2H), 1.98 (d, 2H), 1.61 (m, 2H), 1.41 (s, 9H). MS (EI) for C17 H22N4 O4: 347 (MH+).
1 , 1 -Dimethylethyl 4-(6-(5-bromo-2-carbamoylbenzofuran-3- ylcarbamovDpyrazolor 1 ,5-a1pyrimidin-2-yl)piperidine- 1 -carboxylate
[00793] 1 , 1 -Dimethylethyl 4-(6-(5-bromo-2-carbamoylbenzofuran-3- ylcarbamoyl)pyrazolo[ 1 ,5-a]pyrimidin-2-yl)piperidine- 1 -carboxylate was synthesized in a manner similar to 1 , 1 -dimethylethyl 3 -(5 -bromo-2-carbamoylbenzofuran-3 - ylcarbamoyl)azetidine- 1 -carboxylate wherein 2-( 1 -(tert-butoxycarbonyl)piperidin-4- yl)pyrazolo[l,5-a]pyrimidine-6-carboxylic acid replaced Boc-3-azetidine carboxylic acid. The crude material from this reaction was submitted to the next step without any further purification. MS (EI) for C26 H27 Br N6 O5: 566 (MH+). 1,1-Dimethylethyl 4-(6-(8-bromo-4-oxo-3,4-dihydrobenzofuror3,2-dlpyrimidin-2- yDpyrazolo [ 1 ,5 -alpyrimidin-2-yDpiperidine- 1 -carboxylate [00794] l,l-Dimethylethyl 4-(6-(8-bromo-4-oxo-3,4-dihydrobenzofuro[3,2- d]pyrimidin-2-yl)pyrazolo[l,5-a]pyrimidin-2-yl)piperidine-l -carboxylate was synthesized in a similar manner as to 1,1 -dimethylethyl 4-(8-bromo-4-oxo-3,4- dihydrobenzofuro [3, 2-d]pyrimidin-2-yl)piperidine-l -carboxylate, wherein 1,1- dimethylethyl 4-(6-(5 -bromo-2-carbamoylbenzofuran-3 -ylcarbamoyl)pyrazolo [1,5- a]pyrimidin-2-yl)piperidine-l -carboxylate replaced 1,1 -dimethylethyl 4-(5-bromo-2- carbamoylbenzofuran-S-ylcarbamoy^piperidine-l-carboxylate. The crude material from this reaction was used in the subsequent step without any further purification. MS (EI) for C26 H25 Br N6 O4: 466 (MH+).
8-Bromo-2-(2-piperidin-4-ylpyrazolo[l,5-alpyrimidin-6-yl)[llbenzofuro[3,2- dlpyrimidin-4(3H)-one
[00795] 8-Bromo-2-(2-piperidin-4-ylpyrazolo[l ,5-a]pyrimidin-6- yl)[l]benzofuro[3,2-d]pyrimidin-4(3H)-one (Compound 377) as its hydrochloride salt, was synthesized in a similar manner to 8-bromo-2-piperidin-4- yl[l]benzofuro[3,2-d]pyrimidin-4(3H)-one (Compound 157. 1H NMR (400 MHz, d6-DMSO): 13.20 (br s, IH), 9.78 (s, IH), 9.24 (s, IH), 8.3 l(s, IH), 7.87 (s, 2H), 7.54 (br s, IH), 6.79 (s, IH), 3.29 (m, 2H), 3.22 (m, IH), 3.09 (m, 2H), 2.32 (m, 2H),
1.97 (m, 2H). MS (EI) for C21 H17 Br N6 O2: 466 (MH+).
8-Chloro-2- { [(3 S)-3 -hydroxypyrrolidin- 1 -yllmethyl) \ 1 lbenzofuro [3 ,2-dipyrimidin-
4(3HVone AAGl 2-(4-Chloro-2-cyanophenoxy)acetamide
[00796] 2-(4-Chloro-2-cyanophenoxy)acetamide was synthesized in a manner similar to 2-(4-bromo-2-cyanophenoxy)acetamide 2 wherein 5-chloro-2- hydroxybenzonitrile (commercially available from Prosynth Ltd.,) replaced 5-bromo-
2-hydroxybenzonitrile 1. 1H NMR (400 MHz, d6-DMSO): 7.92 (d, IH), MS (EI) for C9 H7 Cl N 2O2: 211 (MH+).
3 - Amino-5 -chlorobenzofuran-2-carboxamide [00797] 3 -Amino-5 -chlorobenzofuran-2-carboxamide was synthesized in a manner similar to 3 -amino-5 -bromobenzofuran-2-carboxamide 3, wherein 2-(4-chloro-2- cyanophenoxy)acetamide replaced 2-(4-bromo-2-cyanophenoxy)acetamide 2. 1H NMR (400 MHz, d6-DMSO): 7.95 (s, IH), 7.42 (s, 2H), 7.36 (br s, 2H), 6.02 (br s,
2H). MS (EI) for C9 H7 Cl N2 O2: 211 (MH+).
5-Chloro-3-(2-chloroacetamido)benzofuran-2-carboxamide [00798] 5-Chloro-3-(2-chloroacetamido)benzofuran-2-carboxamide was synthesized in a manner similar to 5-bromo-3-(2-chloroacetamido)benzofuran-2- carboxamide 5, wherein 3 -amino-5 -chlorobenzofuran-2-carboxamide replaced 3- amino-5-bromobenzofuran-2-carboxamide 3. 1H NMR (400 MHz, d6-DMSO): 10.76
(s, IH), 8.26 (br s, IH), 8.08 (d, IH), 8.00 (br s, IH), 7.65 (d,lH), 7.55 (dd, IH), 4.52
(s, 2H). MS (EI) for Cn H8 Ci2 N2 O3: 288 (MH+).
8-Chloro-2-(chloromethyl)benzofuror3,2-dlpyrimidin-4(3H)-one [00799] 8-Chloro-2-(chloromethyl)benzofuro[3,2-d]pyrimidin-4(3H)-one was synthesized in a manner similar to 8-bromo-2-(chloromethyl)benzofuro[3,2- d]pyrimidin-4(3H)-one 6, wherein 5-chloro-3-(2-chloroacetamido)benzofuran-2- carboxamide replaced 5-bromo-3-(2-chloroacetamido)benzofuran-2-carboxamide 5.
1H NMR (400 MHz, d6-DMSO): 13.40 (br s, IH), 8.09 (d,lH), 7.91 (d, IH), 7.72 (dd, 2H), 4.66 (s, 2H). MS (EI) for Cn H6 Cl2N2 O2: 270 (MH+).
8-Chloro-2- { [(3 S)-3 -hydroxypyrrolidin- 1 -yl]methyl| [ 1 lbenzofuro [3 ,2-d]pyrimidin-
4(3HVone AAGl [00800] A stirred mixture of 5-chloro-3-(2-chloroacetamido)benzofuran-2- carboxamide (0.988 mmoles, 0.266), triethylamine ( 2,96 mmoles, 0.300 g, 0.41 niL) and (S)-3-hydroxypyrrolidine (2.96 mmoles, 0.408 g, 0.39 mL, commercially available from Acros) and ethanol ( 20 ml) was heated to 80 0C for 18 hours. The reaction mixture was then allowed to room temperature. The solution was filtered through a Millipore Millex-GN 0.20 uM Nylon syringe filter, and purified by preparative reverse phase HPLC (reverse-phase, acetonitrile/water with 0.01% ammonium acetate). The resulting fractions containing the desired peak (of correct molecular weight) were combined and evaporated under reduced pressure to give 0.12 g of 8-chloro-2-{[(3S)-3-hydroxypyrrolidin-l-yl]methyl}[l]benzofuro[3,2- d]pyrimidin-4(3H)-one as its acetate salt. 1U NMR (400 MHz, d6-DMSO): 13.12 (br s, IH), 8.08 (dd, IH), 7.89 (dd, IH), 7.70 (dd, IH), 4.19 (m,lH), 3.68 (s, 2H), 3.35 (br s, IH), 2.79 (m, 2H), 2.53 (m, IH), 2.02 (m, IH), 1.59 (m, IH). MS (EI) for Ci5 Hi4 Cl N3 O3: 319 (MH+). (Compound 63)
2-(2-chlorophenyl)-8-methyl[llbenzofuro[3,2-dlpyrimidin-4(3H)-one [00801] 2-(2-chlorophenyl)-8-methyl[l]benzofuro[3,2-d]pyrimidin-4(3H)-one was synthesized in the same manner as Example 8 wherein 3-amino-5-bromobenzofuran- 2-carboxamide 3 was replaced with 3-amino-5-methyl-benzofuran-2-carboxamide. 3- Amino-5-methyl-benzofuran-2-carboxamide was synthesized in the same manner as Example 1 wherein 5-bromo-2-hydroxybenzonitrile 1 was replaced with 5-methyl-2- hydroxybenzonitrile. 5-methyl-2-hydroxybenzonitrile was synthesized in the same manner as Example AAGl wherein 5-bromo-2-hydroxy-3-methylbenzaldehyde was replaced with 2-hydroxy-5-methylbenzaldehyde. IH NMR (400 MHz, d6-DMSO): 13.28 (s, IH), 7.85 (s, IH), 7.76 (d, IH), 7.70 (dd, IH), 7.65 (m, IH), 7.60 (m, IH), 7.53 (m, 2H), 2.48 (s, 3H). MS (EI) for Ci7HnClN2O2: 312 (MH+). 8-Bromo-2- { [(3 S)- 3 -hydroxypyrrolidin- 1 -yl]methyl| -6-methyl[ 1 ]benzofuro [3 ,2- dlpyrimidin-4(3H)-one AAG2 E/Z -5-Bromo-2-hvdroxy-3-methylbenzaldehvde oxime
Figure imgf000350_0001
[00802] An aqueous solution of hydroxylamine (93.00 mmole, 3.07 g, 6.14 ml, 50% w/w commercially from Alfa Aesar) was added in lot to a stirred solution of 5- bromo-2-hydroxy-3-methylbenzaldehyde (1Og, 46.50 g) (prepared by a literature method - J.Catalysis 2004, 221, 77), in ethanol 200 ml. The mixture was then heated to 100 0C for 2 hours. The reaction mixture was then allowed to cool to room temperature and then poured on crushed ice. The resulting white slurry was then stirred for 30 minutes, filtered off, washed with cold water (2 x 25 ml) and then dried at reduced pressure to give 10.24 g of E/Z -5-bromo-2-hydroxy-3- methylbenzaldehyde oxime as a white solid, which was used in the next step without any further purification. 1U NMR (400 MHz, d6-DMSO): 11.70 (s, IH), 10.40 (s, IH), 8.34 (s, IH), 7.44 (d, IH), 7.32 (d, IH), 2.15 (S, 3H). MS (EI) for C8 H8 Br N O2: 231 (MH+).
5 -Bromo-2-hydroxy-3 -methylbenzonitrile
Figure imgf000351_0001
[00803] Phosphorus oxychloride (108 mmoles 16.66 g, 10.12 mL), ws added in a drop wise fashion to a stirred solution of E/Z -5-bromo-2-hydroxy-3- methylbenzaldehyde oxime (43.46 mmoles, 1Og ) in anhydrous DMF (50 mL) at 5 0C (ice-water bath). Upon completion of addition, the cooling bath was removed and the reaction mixture was stirred for 5 hours. The reaction mixture was the poured onto crushed ice, resulting in the precipitation of a light brown solid. This suspension was stirred for 1 hour and then filtered off, washed with cold water (2 x 20 ml) and dried at reduced pressure to give 7.73 g of 5 -bromo-2-hydroxy-3 -methylbenzonitrile which was used in the next step without any further purification. 1H NMR (400 MHz, d6- DMSO): 10.51 (br s, IH), 9.21 (d, IH), 8.21 (s, IH), 2.18 (s, 3H). MS (EI) for C8 H6 Br N O: 213 (MH+).
Reference: J.Chinese Chemical Society, 1988, 35, 459 2-(4-Bromo-2-cyano-6-methylphenoxy)acetamide
[00804] 2-(4-Bromo-2-cyano-6-methylphenoxy)acetamide was synthesized in a manner similar to 2-(4-bromo-2-cyanophenoxy)acetamide 2, wherein 5-bromo-2- hydroxy-3 -methylbenzonitrile replaced 5-bromo-2-hydroxybenzonitrile 1. 1H NMR (400 MHz, de-DMSO): 7.92 (d, IH), 7.70 (dd, IH), 7.51 (br s, IH), 7.44 (br s, IH), 7.07 (d, IH), 4.68 (s, 2H). MS (EI) for Ci0 H9 Br N2 O2: 270 (MH+). 3 - Amino-5 -bromo-7-methylbenzofuran-2-carboxamide
[00805] 3 -Amino-5 -bromo-7-methylbenzofuran-2-carboxamide was synthesized in a manner similar to 3 -amino-5 -bromobenzofuran-2-carboxamide 3, wherein 2-(4- bromo-2-cyano-6-methylphenoxy)acetamide replaced 2-(4-bromo-2- cyanophenoxy)acetamide 2. 1H NMR (400 MHz, d6-DMSO): 7.80 (s, IH), 7.41 (s, IH), 7.30 (br s, 2H), 5.97 (br s, 2H). 2.50 (s, 3H). MS (EI) for Ci0 H9 Br N2 O2: 270 (MH+). 5-Bromo-3-(2-chloroacetamido)-7-methylbenzofuran-2-carboxamide
[00806] 5-Bromo-3-(2-chloroacetamido)-7-methylbenzofuran-2-carboxamide was synthesized in a manner similar to 5-bromo-3-(2-chloroacetamido)benzofuran-2- carboxamide 5, wherein 3 -amino-5 -bromo-7-methylbenzofuran-2-carboxamide replaced 3 -amino-5 -bromobenzofuran-2-carboxamide 3. 1H NMR (400 MHz, d6- DMSO): 10.72 (br s, IH), 8.23 (s, IH), 8.01 (d, 2H), 7.51 (s, IH), 4.51 (s, 2H), 2.51 (s, 3H). MS (EI) for C12 Hi0 Br Cl N2 O3: 346 (MH+). 8-Bromo-2-(chloromethyl)-6-methylbenzofuro[3,2-dlpyrimidin-4(3H)-one [00807] 8-Bromo-2-(chloromethyl)-6-methylbenzofuro[3,2-d]pyrimidin-4(3H)-one was synthesized in a manner similar to 8-bromo-2-(chloromethyl)benzofuro[3,2- d]pyrimidin-4(3H)-one 6, wherein 5-bromo-3-(2-chloroacetamido)-7- methylbenzofuran-2-carboxamide replaced 5-bromo-3-(2- chloroacetamido)benzofuran-2-carboxamide 5. 1H NMR (400 MHz, dβ-DMSO): 13.36 (br s, IH), 7.95 (d, IH), 7.67 (d, IH), 4.65 (s, 2H), 2.53 (s, 3H). MS (EI) for Ci2 H8 Br Cl N2 O2: 328 (MH+). 8-Bromo-2- { [(3 S)- 3 -hydroxypyrrolidin- 1 -yllmethyl) -6-methyl[ 1 lbenzofuro [3 ,2- d1pyrimidin-4(3H)-one AAG2
[00808] A stirred mixture of 8-bromo-2-(chloromethyl)-6-methylbenzofuro[3,2- d]pyrimidin-4(3H)-one (0.915 mmoles, 0.3 g), triethylamine ( 2.74 mmoles, 0.278 g, 0.38 mL), (S)-3-hydroxypyrrolidine (2.74 mmoles, 0.378 g, 0.36 mL, commercially available from Acros) and ethanol ( 20 ml) was heated to 80 0C for 18 hours. The reaction mixture was then allowed to cool to room temperature. The solution was filtered through a Millipore Millex-GN 0.20 uM Nylon syring filter, and purified by preparative reverse phase HPLC (reverse-phase, acetonitrile/water with 0.01% ammonium acetate). The resulting fractions containing the desired peak (of correct molecular weight) were combined and evaporated under reduced pressure to give 0.101 g of 8-bromo-2-{[(3S)-3-hydroxypyrrolidin-l-yl]methyl}-6- methyl[l]benzofuro[3,2-d]pyrimidin-4(3H)-one as its acetate salt. 1H NMR (400 MHz, de-DMSO): 13.04 (br s, IH), 7.98 (s, IH), 7.64 (s, IH), 4.19 (s, IH), 3.67 (s, 2H), 3.44 (br s, IH), 2.50 (s, 3H), 2.02 (m, 2H), 1.59 (m, 2H). MS (EI) for Ci6 Hi6 Br N3 O3: 379 (MH+).
Figure imgf000353_0001
(Compound 289) 8-bromo-2-(l-methylpyrrolidin-3-yl)[llbenzofuro[3,2-dlpyrimidin-4(3H)-one
[00809] 8-bromo-2-pyrrolidin-3-yl[l]benzofuro[3,2-d]pyrimidin-4(3H)-one (Compound 186) (50 mg, 0.14 mmol) was added to anhydrous DMF (3 ml) and stirred to ensure complete dissolution of the solid. The stirred reaction mixture was treated with 37 % aqueous formaldehyde (10 equivalents) and glacial acetic acid (cat amount). The reaction mixture was stirred at room temperature for 5 minutes. The stirred reaction mixture was then treated with sodium triacetoxyborohydride (10 equivalents, commercially available from BASF Corporation ) and additional glacial acetic acid (1 drop). The reaction mixture was then stirred at room temperature for 30 minutes and shown to be complete by LC-MS. The solution was filtered through a Millipore Millex-GN 0.20 uM Nylon syring filter, and the resulting solution was submitted for preparative reverse phase HPLC (reverse-phase, acetonitrile/water with 0.01% ammonium acetate), followed by concentration in vacuo and lyophilization afforded 40 mg of 8-bromo-2-(l-methylpyrrolidin-3-yl)[l]benzofuro[3,2-d]pyrimidin- 4(3H)-one (acetate salt) as a white solid. 1U NMR (400 MHz, d6-DMSO): 8.22 (d, IH), 8.18 (s, IH), 7.82 (s, 2H), 3.55-3.43 (m, IH), 2.98 (t, IH), 2.85 (dd, IH), 2.75- 2.65 (m, 2H), 2.57 (s, 3H), 2.28-2.15 (m, IH). MS (EI) for Ci5Hi4BrN3O2: 348 (MH+). RCOOH, HATU , DMF
Figure imgf000354_0001
Figure imgf000354_0002
wherein R is as described within the compounds within this example.
(Compound 203)
2-(l-acetylpiperidin-4-yl)-8-bromoπibenzofuror3,2-dlpyrimidin-4(3H)-one [00810] To a solution of 8-bromo-2-piperidin-4-yl[l]benzofuro[3,2-d]pyrimidin- 4(3H)-one hydrochloride salt (100 mg, 0.28 mmol) in DMF (3 mL) was added acetic acid (1.5 eq), triethylamine (0.1 mL) and HATU (1.2 eq). The reaction mixture was stirred at room temp, until the starting material was consumed. Purification by preparative HPLC resulting in 68 mg of 2-(l-acetylpiperidin-4-yl)-8- bromo[l]benzofuro[3,2-d]pyrimidin-4(3H)-one. 1H NMR (400 MHz, d6-DMSO):
12.95 (s, IH), 8.18 (s, IH), 7.82 (s, 2H), 4.44 (d, IH), 3.95 (d, IH), 3.18-2.95 (m, 2H), 2.64-2.57 (m, IH), 1.95-1.63 (m, 4H); MS (EI) for Ci7Hi6BrN3O3: 390 (MH+).
(Compound 206)
8-bromo-2-[ 1 -(N,N-dimethyl-beta-alanyl)piperidin-4-yll [ 1 ]benzofuro[3 ,2- dlpyrimidin-4(3H)-one
[00811] 8-bromo-2-[ 1 -(N,N-dimethyl-beta-alanyl)piperidin-4-yl] [1 ]benzofuro[3,2- d]pyrimidin-4(3H)-one was synthesized in a manner similar Example 34 wherein acetic acid was substituted with 3-(dimethylamino)propanoic acid. Purification by preparative HPLC resulting in 54 mg of 8-bromo-2-[l-(N,N-dimethyl-beta- alanyl)piperidin-4-yl][l]benzofuro[3,2-d]pyrimidin-4(3H)-one. 1H NMR (400 MHz, de-DMSO): 8.24 (s, IH), 8.18 (s, IH), 7.81 (s, 2H), 4.55 (d, IH), 4.04 (d, IH), 3.23-
2.95 (m, 4H),
2.69-2.58 (m, 3H), 2.23 (s, 6H), 2.00-1.90 (m, 2H), 1.85-1.63 (m, 2H); MS (EI) for
C20H23BrN4O3: 447 (MH+). (Compound 207)
8-bromo-2- { 1 -r4-(dimethylamino)butanovHpiperidin-4-yl| \ 1 lbenzofuroP ,2- d"|pyrimidin-4(3H)-one [00812] 8-bromo-2- { 1 -[4-(dimethylamino)butanoyl]piperidin-4- yl}[l]benzofuro[3,2-d]pyrimidin-4(3H)-one was synthesized in a manner similar Example 34 wherein acetic acid was substituted with 4-(dimethylamino)butanoic acid. Purification by preparative HPLC resulting in 80 mg of 8-bromo-2-{l-[4- (dimethylamino)butanoyl]piperidin-4-yl}[l]benzofuro[3,2-d]pyrimidin-4(3H)-one. 1H NMR (400 MHz, d6-DMSO): 8.21 (s, IH), 8.17 (s, IH), 7.82 (s, 2H), 4.53 (d, IH), 4.03 (d, IH), 3.23-2.95 (m, 4H), 2.70-2.58 (m, IH), 2.40-2.25 (m, 3H), 2.23 (s, 6H), 2.00-1.90 (m, 2H), 1.81-1.63 (m, 3H); MS (EI) for C2IH25BrN4O3: 461 (MH+).
(Compound 208)
2-[l-(lH-benzimidazol-5-ylcarbonyl)piperidin-4-yll-8-bromo[llbenzofuro[3,2- d"|pyrimidin-4(3H)-one [00813] 2-[l-(lH-benzimidazol-5-ylcarbonyl)piperidin-4-yl]-8- bromo[l]benzofuro[3,2-d]pyrimidin-4(3H)-one was synthesized in a manner similar Example 34 wherein acetic acid was substituted with 7H-benzo[d]imidazole-5- carboxylic acid. Purification by preparative HPLC resulting in 34 mg of 2-[1-(1H- benzimidazol-5-ylcarbonyl)piperidin-4-yl]-8-bromo[l]benzofuro[3,2-d]pyrimidin- 4(3H)-one. 1H NMR (400 MHz, d6-DMSO): 12.95 (s, IH), 8.40 (s, IH), 8.21 (s, IH), 8.19 (s, IH), 7.80 (s, 2H), 7.70 (d, 2H), 7.30 (d, IH), 4.57 (s, IH), 3.93-3.90 (m, IH), 3.15-3.00 (m, 3H), 2.05-1.78 (m, 4H); MS (EI) for C23Hi8BrN5O3: 492 (MH+).
(Compound 209)
8-bromo-2-{l-[3-(methyloxy)propanoyllpiperidin-4-yl|[llbenzofuro[3,2- dlpyrimidin-4(3H)-one [00814] 8-bromo-2-{l-[3-(methyloxy)propanoyl]piperidin-4-yl}[l]benzofuro[3,2- d]pyrimidin-4(3H)-one was synthesized in a manner similar Example 34 wherein acetic acid was substituted with 3-methoxypropanoic acid. Purification by preparative HPLC resulting in 76 mg of 8-bromo-2-{l-[3-(methyloxy)propanoyl]piperidin-4- yl}[l]benzofuro[3,2-d]pyrimidin-4(3H)-one. 1H NMR (400 MHz, d6-DMSO): 12.95 (s, br, IH), 8.15 (s, IH), 7.81 (s, 2H), 4.45 (d, IH), 4.00 (d, IH), 3.60-3.55 (m, 2H), 3.23-3.18 (m, 3H), 3.10-2.95 (m, 2H), 2.64-2.55 (m, 3H), 1.98-1.80 (m, 4H); MS (EI) for Ci9H20BrN3O4: 434 (MH+).
(Compound 210)
8-bromo-2-[ 1 -(N,N-dimethylglvcyl)piperidin-4-yll [ 1 lbenzofuro[3 ,2-dlpyrimidin- 4(3H)-one
[00815] 8-bromo-2-[l-(N,N-dimethylglycyl)piperidin-4-yl][l]benzofuro[3,2- d]pyrimidin-4(3H)-one was synthesized in a manner similar Example 34 wherein acetic acid was substituted with 2-(dimethylamino)acetic acid. Purification by preparative HPLC resulting in 80 mg of 8-bromo-2-[l-(N,N- dimethylglycyl)piperidin-4-yl][l]benzofuro[3,2-d]pyrimidin-4(3H)-one. 1H NMR (400 MHz, de-DMSO): 8.21 (s, IH), 8.18 (s, IH), 7.82 (s, 2H), 4.50 (d, IH), 4.18 (d, IH), 3.21-2.95 (m, 4H), 2.66-2.60 (m, IH), 2.20 (s, 3H), 2.00-1.95 (m, 2H), 1.81-1.60 (m, 2H); MS (EI) for Ci9H2IBrN4O3: 433 (MH+).
(Compound 233)
8-bromo-2-[l-(tetrahydrofuran-3-ylcarbonyl)piperidin-4-yll[llbenzofuro[3,2- d"|pyrimidin-4(3H)-one [00816] 8-bromo-2-[ 1 -(tetrahydrofuran-3-ylcarbonyl)piperidin-4- yl][l]benzofuro[3,2-d]pyrimidin-4(3H)-one was synthesized in a manner similar Example 34 wherein acetic acid was substituted with tetrahydrofuran-3-carboxylic acid. Purification by preparative HPLC resulting in 8-bromo-2-[l-(tetrahydrofuran-3- ylcarbonyl)piperidin-4-yl][l]benzofuro[3,2-d]pyrimidin-4(3H)-one. 1H NMR (400 MHz, d6-DMSO): 8.15 (s, IH), 7.81 (s, 2H), 4.45 (d, IH), 4.00 (d, IH), 3.95-3.87 (m, IH), 3.80-3.70 (m, 2H), 3.10-3.00 (m, 2H), 2.65-2.60 (m, IH), 1.90-1.80 (m, 4H), 1.75-1.65 (m, 3H); MS (EI) for C20H20BrN3O4: 446 (MH+).
(Compound 234)
8-bromo-2-[ 1 -(phenylcarbonyl)piperidin-4-yll [ 1 "IbenzofuroP ,2-d1pyrimidin-4(3H)- one [00817] 8-bromo-2-[l-(phenylcarbonyl)piperidin-4-yl][l]benzofuro[3,2- d]pyrimidin-4(3H)-one was synthesized in a manner similar Example 34 wherein acetic acid was substituted with benzoic acid. Purification by preparative HPLC resulting in 8-bromo-2- [ 1 -(phenylcarbonyl)piperidin-4-yl] [ 1 ]benzofuro [3 ,2- d]pyrimidin-4(3H)-one. 1H NMR (400 MHz, d6-DMSO): 8.18 (s, IH), 8.00 (d, 2H), 7.82 (d, 2H), 7.76-7.58 (m, 3H), 3.98-3.88 (m, 2H), 3.18-2.95 (m, 2H), 2.64-2.57 (m, IH), 1.95-1.63 (m, 4H); MS (EI) for C22Hi8BrN3O3: 452 (MH+).
(Compound 236)
8-bromo-2-[ 1 -(pyridin-4-ylcarbonyl)piperidin-4-yll [ 1 lbenzofuro[3 ,2-dlpyrimidin- 4(3HVone [00818] 8-bromo-2-[ 1 -(pyridin-4-ylcarbonyl)piperidin-4-yl] [ 1 ]benzofuro[3,2- d]pyrimidin-4(3H)-one was synthesized in a manner similar Example 34 wherein acetic acid was substituted with isonicotinic acid. Purification by preparative HPLC resulting in 8-bromo-2-[ 1 -(pyridin-4-ylcarbonyl)piperidin-4-yl] [ 1 ]benzofuro[3,2- d]pyrimidin-4(3H)-one. 1H NMR (400 MHz, d6-DMSO): 8.18 (s, IH), 8.00 (m, 2H), 7.73 (d, 2H), 7.58 (d, 2H), 3.93-3.80 (m, 2H), 3.20-3.00 (m, 2H), 1.99-1.70 (m, 4H); MS (EI) for Ci2Hi7BrN4O3: 453 (MH+).
(Compound 237) 8-bromo-2-[ 1 -(phenylacetyl)piperidin-4-yll [ 1 lbenzofuro[3 ,2-d1pyrimidin-4(3H)-one [00819] 8-bromo-2-[ 1 -(phenylacetyl)piperidin-4-yl] [ 1 ]benzofuro[3,2-d]pyrimidin- 4(3H)-one was synthesized in a manner similar Example 34 wherein acetic acid was substituted with 2-phenylacetic acid. Purification by preparative HPLC resulting in 8- bromo-2-[l-(phenylacetyl)piperidin-4-yl][l]benzofuro[3,2-d]pyrimidin-4(3H)-one. 1H NMR (400 MHz, d6-DMSO): 8.18 (s, IH), 8.00 (d, 2H), 7.45-7.33 (m, 3H), 7.28-7.19 (m, 2H), 3.98-3.90 (m, 2H), 3.85 (s, 2H), 3.18-2.95 (m, 2H), 2.64-2.57 (m, IH), 1.95- 1.63 (m, 4H); MS (EI) for C23H20BrN3O3: 466 (MH+).
(Compound 290)
8-bromo-2-ri-(pyridin-4-ylmethyl)pyrrolidin-3-ylirilbenzofuror3,2-dlpyrimidin- 4(3HVone
[00820] 8-bromo-2-[l-(pyridin-4-ylmethyl)pyrrolidin-3-yl][l]benzofuro[3,2- d]pyrimidin-4(3H)-one was synthesized in a manner similar to Compound 289. Purification by preparative HPLC resulting in 8-bromo-2-[l-(pyridin-4- ylmethyl)pyrrolidin-3-yl][l]benzofuro[3,2-d]pyrimidin-4(3H)-one. 1H NMR (400 MHz, de-DMSO): 8.60 (s, 2H), 8.22 (d, IH), 8.18 (s, IH), 7.82 (s, 2H), 7.48 (s, 2H), 3.98 (s, 2H), 3.57-3.45 (m, IH), 3.07-2.95 (m, IH), 2.89-2.65 (m, 3H), 2.35-2.22 (m, 2H). MS (EI) for C20Hi7BrN4O2: 425 (MH+).
2-(5-Amino-2-chlorophenyl)-8-bromo[llbenzofuro[3,2-dlpyrimidin-4(3H)-one 391. AAG3 1 , 1 -Dimethylethyl 3-(5-bromo-2-carbamoylbenzofuran-3-ylcarbamoyl)-4- chlorophenylcarbamate
[00821 ] 1,1 -Dimethylethyl 3 -(5 -bromo-2-carbamoylbenzofuran-3 -ylcarbamoyl)-4- chlorophenylcarbamate was synthesized in a manner similar to 1,1 -dimethylethyl 3- (5-bromo-2-carbamoylbenzofuran-3-ylcarbamoyl)azetidine- 1 -carboxylate wherein 5- (tert-butoxycarbonylamino)-2-chlorobenzoic acid (commercially available from CNH Technologies Inc..) replaced Boc-3-azetidine carboxylic acid. The crude material from this reaction was submitted to the next step without any further purification. 1H
NMR (400 MHz, d6-DMSO): 10.73 (br s, IH), 9.76 (br s, IH), 8.30 (s, IH), 8.26 (s,lH), 8.00 (br s, IH), 7.88 (s, 2H), 7.70 (dd, IH), 7.63 (d, IH), 7.57 (dd, IH), 7.49 (d, IH), 1.48 (s, 9H). MS (EI) for C21 Hi9 Br Cl N3 O5: 509 (MH+). 2-(5-Amino-2-chlorophenyl)-8-bromorilbenzofuror3,2-dlpyrimidin-4(3H)-one
Figure imgf000358_0001
not isolated
[00822] Crude 2-(5-amino-2-chlorophenyl)-8-bromo[l]benzofuro[3,2-d]pyrimidin- 4(3H)-one AAG3 (15.92 mmoles, 8.1 g), was dissolved in ethanol ( 200 ml) and treated with aqueous IM potassium hydroxide solution (60 mmoles, 60ml). The stirred reaction mixture was then refluxed for 18 hours. The reaction mixture was then allowed to cool to ca. 60 0C. The warm reaction mixture was then acidified to pH =1, by the drop wise addition of concentrated hydrochloric acid, which resulted in the formation of a white precipitate. The resulting suspension was then diluted with additional ethanol (100ml) and heated to 100 0C for a further 8 hours. The reaction mixture was then allowed to cool and the resulting solid was filtered off, washed with cold ethanol and dried under reduced pressure to give 4.97 of 2-(5-amino-2- chlorophenyl)-8-bromo[l]benzofuro[3,2-d]pyrimidin-4(3H)-one as its hydrochloride salt, as a white solid. 1U NMR (400 MHz, d6-DMSO): 13.44 (br s, IH), 8.25 (7.89 (dd, IH), 7.85 (dd, IH), 7.56 (d, IH), 7.35 (s, IH), 7.28 (dd, IH), 6.82 (br s, 3H).MS (EI) for C16 H9 Br Cl N3 02: 391 : (MH+). 8-Bromo-6-methyl-2-((4-methylpiperazin-l-yl)methyl)benzofuro[3,2-dlpyrimidin- 4(3HVone AAG4
[00823] A stirred mixture of 8-bromo-2-(chloromethyl)-6-methylbenzofuro[3,2- d]pyrimidin-4(3H)-one (0.978 mmoles, 0.32 g), triethylamine (2.93 mmoles, 0.73 g, 0.41 mL), N-methylpiperaazine (2.93 mmoles, 0.29 g, 0.28 mL, commercially available from Acros) and ethanol (20 ml) was heated to 80 0C for 18 hours. The reaction mixture was then allowed to cool to room temperature. The solution was filtered through a Millipore Millex-GN 0.20 uM Nylon syring filter, and purified by preparative reverse phase HPLC (reverse-phase, acetonitrile/water with 0.01% ammonium acetate). The resulting fractions containing the desired peak (of correct molecular weight) were combined and evaporated under reduced pressure to give 0.168g of 8-bromo-6-methyl-2-((4-methylpiperazin-l-yl)methyl)benzofuro[3,2- d]pyrimidin-4(3H)-one as its acetate salt.
1H NMR (400 MHz, d6-DMSO): 12.60 (br s, IH), 7.99 (s, IH), 7.80 (s, IH), 3.50 (s, 2H),
3.39, (s, 4H), 2.54 (s, 3H), 2.22 (s, 4H), 2.14 (s, 3H). MS (EI) for Ci7 Hi9 Br N4 O2: 392 (MH+).
Compound 534
N-r2-(Aminocarbonyl)-5-bromo-l-benzofuran-3-yllpiperidine-2-carboxamide hydrochloride
[00824] DL-pipecolinic acid (5.0 g, 38.7 mmol) was suspended in dichloromethane (50 mL) and cooled in a -100C ice bath. Phosphorus pentachloride (8.14 g, 39.1 mmol) was added in one portion. The reaction mixture was stirred in the cold bath for 1 h to give a clear solution. Another reaction flask was charged with 3-amino-5- chloro-l-benzofuran-2-carboxamide, (2.45 g, 9.6 mmol) was suspended in dichloromethane (25 mL) and cooled in a -100C ice/salt bath. The solution of DL- pipecolinic acid chloride hydrochloride was added dropwise over 5 min. The reaction mixture was allowed to warm to room temperature and was stirred overnight. The precipitate was isolated by filtration using additional dichloromethane (50 mL) to wash the material from the reaction flask. The white solid was washed with dichloromethane (3 x 20 mL) and dried under vacuum to give 4.05 g of (N-[2- (aminocarbonyl)-5 -bromo- 1 -benzofuran-3 -yl]piperidine-2-carboxamide hydrochloride as a light grey powder. The material was used in the next step without any further purification. MS (EI) for Ci5Hi6BrN3O3: 366 (MH+).
8-Bromo-2-piperidin-2-virHbenzofuror3,2-dlpyrimidin-4(3H)-one hydrochloride [00825] N- [2-(Aminocarbonyl)-5 -bromo- 1 -benzofuran-3 -yl]piperidine-2- carboxamide hydrochloride (4.0 g , 9.9 mmol)was suspended in ethanol, (100 mL) and sodium hydroxide (25 mL, 2.0 M, 50 mmol) was added which caused all the material to dissolve. The reaction mixture was stirred at 45°C for 4 h. After cooling to room temperature, the reaction mixture was further cooled to 0-50C in an ice bath. Hydrochloric acid (6M) was added slowly to lower the pH to 2. The reaction mixture was removed from the ice bath and allowed to stand at 4°C in a refrigerator for 16 hours. The precipitate was removed by filtration. The filtrate was concentrated under vacuum, the residue was stirred in ice water (25 mL) and the solid was isolated by filtration. The solid was air dried for 5 h and then stirred in ethyl acetate at 500C for 48 h. The solid was isolated by filtration, washed with ethyl acetate (2 x 30 mL) and dried under vacuum to give 1.27 g (33% yield, >95% purity). MS (EI) for Ci5Hi4BrN3O2: 348 (MH+).
1H NMR (400 MHz, d6-DMSO): 1349 (MH+). 13.61 (br s, IH), 8.10 (d, IH), 7.88 (s, IH), 7.86 (d, IH), 7.84 (d, IH), 4.39 (t, IH), 4.43 (d, IH), 3.56 (br s, 2H), 3.04 (m, IH), 2.31 (IH), 1.73 (m, 2H), 1.92 (m ,2H), 1.52 (m, 2H). Compound 535
2-(l-amino-2-phenylethyl)-8-bromo[llbenzofuro[3,2-(i]pyrimidin-4(3H)-one N- [2-(aminocarbonyl)-5 -bromo- 1 -benzofuran-3 -yl] -Mx- ([(U -dimethylethyDoxy] carbony 11 pheny lalaninamide [00826] To a 250 mL round-bottomed flask equipped with a magnetic stir bar was added N-{[(l,l-dimethylethyl)oxy]carbonyl}phenylalanine (16.7 g, 63.0 mmol), dichloromethane (160 mL), and pyridine (5.0 mL, 61.8 mmol). This mixture was cooled to -10 0C and stirred at this temperature for 15 min. Cyanuric fluoride (13.5 mL, 157.4 mmol) was added via syringe and the resulting mixture was allowed to stir at -10 0C for 1 h. The reaction was then diluted with dichloromethane (250 mL) and quenched slowly with water (250 mL) at 0 0C. The solids were filtered and the layers were separated and concentrated in vacuo. This crude reaction was then redissolved in dichloromethane (80 mL) and dimethylacetamide (80 mL). 3-Amino-5-bromo-l- benzofuran-2-carboxamide (4.0 g, 15.7 mmol) and pyridine (3.8 mL, 47.2 mmol) was added and the reaction was monitored by LCMS. Upon completion, the reaction was poured into 1 M aqueous hydrochloric acid (100 mL) and the aqueous layer was extracted with ethyl acetate (4 x 100 mL). The organics were then washed with 0.5 M aqueous sodium hydroxide (100 mL), water (100 mL), brine (100 mL) and then dried over sodium sulfate. The ethyl acetate was removed under vacuum to give the product, M- [2-(aminocarbonyl)-5 -bromo- 1 -benzofuran-3 -yl] -Mx- {[(1,1- dimethylethyl)oxy] carbony 1} pheny lalaninamide. The crude product was submitted to the next step without further purification. MS (EI) for C23H24BrN3O5: 502 (MH+). M- [2-(aminocarbonyl)-5 -bromo- 1 -benzofuran-3 -yllphenylalaninamide hydrochloride [00827] N-[2-(Aminocarbonyl)-5-bromo- 1 -benzofuran-3-yl]-Mx- {[(1 , 1 - dimethylethyl)oxy]carbonyl}phenylalaninamide was dissolved in ethyl acetate (80 niL) and methanol (80 niL). The mixture was then treated with 4 M hydrochloric acid in dioxane (80 mL, 315 mmol) and the reaction was monitored via LCMS. Upon completion, the solids were filtered to give Λ/-[2-(aminocarbonyl)-5-bromo-l- benzofuran-3-yl]phenylalaninamide hydrochloride. The crude material was submitted to the next step without purification. MS (EI) for Ci8Hi6BrN3O3: 402 (MH+). 2-(l-amino-2-phenylethyl)-8-bromo[llbenzofuro[3,2-(i]pyrimidin-4(3H)-one [00828] N- [2-(Aminocarbonyl)-5 -bromo- 1 -benzofuran-3 -yljphenylalaninamide hydrochloride was dissolved in ethanol (160 mL) and treated with 1 M aqueous sodium hydroxide (80 mL, 78.7 mmol). The reaction was stirred at 60 0C. Upon completion by LCMS, the reaction was cooled to 0 0C and the pH was adjusted to 2. The precipitate was filtered to give 2-(l -amino-2 -phenylethyl)-8- bromo[l]benzofuro[3,2-J]pyrimidin-4(3H)-one (788 mg, 13% yield for 3 steps). 1H NMR (400 MHz, d6-DMSO): 8.87 (b s, IH), 8.12 (s, IH), 7.90 (m, 2H), 7.27 (m, 5H), 4.6 (m, IH), 3.3 (m, 2H). MS (EI) for Ci8Hi4BrN3O2: 384 (MH+).
Compound 536
2-(l -amino-2- {4-IYphenylmethvDoxyiphenvU ethyl)-8-bromor 11benzofuror3,2- άπpyrimidin-4(3//)-one [00829] 2-(l -Amino-2- {4-[(phenylmethyl)oxy]phenyl} ethyl)-8- bromo[l]benzofuro[3,2-d]pyrimidin-4(3H)-one was synthesized in a manner similar to Compound 535, wherein N-{[(l,l-dimethylethyl)oxy]carbonyl}-0- (phenylmethyl)tyrosine replaced N- { [( 1 , 1 - dimethylethyl)oxy]carbonyl}phenylalanine. 1H NMR (400 MHz, d6-DMSO): 8.12 (s, IH), 7.90 (m, 2H), 7.37 (m, 5H), 7.15 (d, 2H), 6.93 (d, 2H), 5.04 (s, 2H) 4.58 (t, IH), 3.27 (m, 2H). MS (EI) for C25H20BrN3O3: 490 (MH+).
Compound 537
2-[(iy)-l-amino-3-phenylpropyll-8-bromo[llbenzofuro[3,2-άπpyrimidin-4(3H)-one
[00830] 2-[(15)-l-Amino-3-phenylpropyl]-8-bromo[l]benzofuro[3,2-(i]pyrimidin- 4(3H)-one was synthesized in a manner similar to Compound 535, wherein (2S)-2- ({[(l,l-dimethylethyl)oxy]carbonyl}amino)-4-phenylbutanoic acid replaced for N- {[(l,l-dimethylethyl)oxy]carbonyl}phenylalanine. 1H NMR (400 MHz, d6-DMSO): 8.11 (s, IH), 7.90 (m, 2H), 7.20 (m, 5H), 4.47 (t, IH), 2.70 (m, 2H), 2.30 (m, 2H). MS (EI) for Ci9Hi6BrN3O2: 398 (MH+).
Compound 538
2-ri-amino-2-(2-thienyl)ethyll-8-bromorilbenzofuror3,2-άπpyrimidin-4(3H)-one [00831] 2-[l-Amino-2-(2-thienyl)ethyl]-8-bromo[l]benzofuro[3,2-(i]pyrimidin- 4(3H)-one was synthesized in a manner similar to Compound 535, wherein N-{[(1,1- dimethylethyl)oxy] carbonyl} -3 -(2-thieny l)alanine replaced N- { [( 1 , 1 - dimethylethyl)oxy]carbonyl}phenylalanine. 1H NMR (400 MHz, d6-DMSO): 8.1 (s, IH), 7.80 (m, 2H), 7.29 (dd, IH), 6.90 (m, 2H), 4.09 (t, IH), 3.28 (m, 2H). MS (EI) for Ci6Hi2BrN3O2S: 390 (MH+).
Compound 539
2-{l-amino-2-r4-(methyloxy)phenyllethvU-8-bromoπibenzofuror3,2-άπpyrimidin- 4(3HVone
[00832] 2- { 1 -Amino-2-[4-(methyloxy)phenyl]ethyl} -8-bromo[ 1 ]benzofuro[3,2- <i]pyrimidin-4(3H)-one was synthesized in a manner similar to Compound 535, wherein N-{[(l,l-dimethylethyl)oxy]carbonyl}-O-methyltyrosine replaced N-{[(1,1- dimethylethyl)oxy]carbonyl}phenylalanine. 1H NMR (400 MHz, d6-DMSO): 8.13 (s, IH), 7.80 (m, 2H), 7.11 (d, 2H), 6.82 (d, 2H), 4.13 (t, IH), 3.69 (s, 3H), 3.15 (m, IH), 2.96 (m, IH). MS (EI) for Ci9Hi6BrN3O3: 414 (MH+). Compound 540
2-[(lS)-l-amino-2-methylpropyll-8-bromo[llbenzofuro[3,2-dlpyrimidin-4(3H)-one
[00833] 2-[(lS)-l-Amino-2-methylpropyl]-8-bromo[l]benzofuro[3,2-d]pyrimidin- 4(3H)-one was synthesized in a manner similar to Compound 535, wherein N-(tert- butoxycarbonyl)-L-valine replaced N- {[(1,1 - dimethylethyl)oxy]carbonyl}phenylalanine. 1H NMR (400 MHz, d6-DMSO): 8.10 (dd, IH), 7.73 (m, 2H), 6.56 (br s, 3H), 3.61 (d, IH), 2.10 (m, IH), 0.91 (d, 3H), 0.85 (d, 3H). MS (EI) for Ci4Hi4BrN3O2: 336 (MH+).
Compound 541 2-[amino(cyclohexyl)methyll-8-bromo[llbenzofuro[3,2- dlpyrimidin-4(3H)-one [00834] 2-[Amino(cyclohexyl)methyl]-8-bromo[l]benzofuro[3,2-d]pyrimidin- 4(3H)-one was synthesized in a manner similar to Compound 535, wherein [(tert- butoxycarbonyl)amino](cyclohexyl)acetic acid replaced N- {[(1,1 - dimethylethyl)oxy]carbonyl}phenylalanine. 1H NMR (400 MHz, d6-DMSO): 8.08 (s, IH), 7.72 (m, 2H), 6.58 (br s, 3H), 3.61 (d, IH), 1.80-1.55 (m, 5H), 1.40 (d, IH), 1.10-1.05 (m, 5H). MS (EI) for Ci7Hi8BrN3O2: 376 (MH+).
Compound 542 l-aminocyclohexanecarbonyl chloride hydrochloride
[00835] 1-Aminocyclohexanecarboxylic acid (1785 mg, 12.5 mmol) was suspended in dichloromethane (50 rnL) and hydrogen chloride gas was bubbled through the suspension for 2 min. The solvent was removed under vacuum to give 1- aminocyclohexane-carboxylic acid hydrochloride as a white solid. Phosphorus pentachloride (3150 mg, 15.1 mmol) was suspended in acetonitrile (50 mL) and the solid 1-aminocyclohexane-carboxylic acid hydrochloride was added in one portion. The solids dissolved quickly and a precipitate formed. The reaction mixture was stirred for 3 h under a nitrogen atmosphere. The precipitate was filtered off, washed with acetonitrile (10 mL) and dried under vacuum to give 2.20 g (89% yield) of 1- aminocyclohexanecarbonyl chloride hydrochloride as a white solid. The material was used without further purification.
3 - { IY 1 -aminocyclohexyDcarbonvnamino I -5 -bromo- 1 -benzofuran-2-carboxamide hydrochloride [00836] 3 -Amino-5 -bromo- 1 -benzofuran-2-carboxamide (510 mg, 2.0 mmol) was dissolved in N,N-dimethylacetamide (10 mL) and l-aminocyclohexanecarbonyl chloride hydrochloride (600 mg, 3.0 mmol) was added in one portion. The reaction was complete within 5 min. The reaction mixture was diluted with acetonitrile (15 mL) and stirred for 5 min. The precipitate was filtered off, washed with acetonitrile (5 mL) and dried under vacuum to give 692 mg (83% yield) of 3-{[(l- aminocyclohexy^carbonyl] amino } -5 -bromo- 1 -benzofuran-2-carboxamide hydrochloride as a white solid. MS (EI) for Ci6Hi8BrN3O3: 380 (MH+). 2-(l-aminocyclohexyl)-8-bromo[llbenzofuro[3,2-dlpyrimidin-4(3H)-one [00837] 3 - { [( 1 - Aminocyclohexyl)carbonyl] amino } -5 -bromo- 1 -benzofuran-2- carboxamide hydrochloride (685 mg, 1.6 mmol) was suspended in ethanol (10 mL) and sodium hydroxide (8.3 mL, 1.0 M, 8.3 mmol) was added to form a clear solution. The reaction was stirred at 85 0C for 2.5 h. After cooling to room temperature, the pH of the reaction mixture was lowered to 8 by addition of 6 M hydrochloric acid over 20 min. The precipitate was filtered off, washed with water (2 mL) and acetonitrile (2 niL), and dried under vacuum to give 466 mg (78% yield) of 2-(l-aminocyclohexyl)- 8-bromo[l]benzofuro[3,2-d]pyrimidin-4(3H)-one as a white solid. 1H NMR (400 MHz, de-DMSO): 8.04 (s, IH), 7.67 (m, 2H), 7.31 (br s, 3H), 2.21-2.14 (m, 2H), 1.71- 1.55 (m, 7H), 1.33 (m, IH). MS (EI) for Ci6Hi8BrN3O3: 380 (MH+). Compound 543
2-(l-aminocyclopentyl)-8-bromo[llbenzofuro[3,2-dlpyrimidin-4(3H)-one
[00838] 2-(l-Aminocyclopentyl)-8-bromo[l]benzofuro[3,2-d]pyrimidin-4(3H)-one was synthesized in a manner similar to Compound 543, wherein 1- aminocyclopentane carboxylic acid replaced 1-aminocyclohexanecarboxylic acid. 1H NMR (400 MHz, d6-DMSO): 8.01 (dd, IH), 7.66 (m, 2H), 7.55 (br s, 3H), 2.33 (m, 2H), 1.84 (m, 6H). MS (EI) for Ci5Hi4BrN3O2: 348 (MH+).
Compound 544
2-(l-aminocyclobutyl)-8-bromo[llbenzofuro[3,2-dlpyrimidin-4(3H)-one
[00839] 2-(l-Aminocyclobutyl)-8-bromo[l]benzofuro[3,2-d]pyrimidin-4(3H)-one was synthesized in a manner similar to Compound 543, wherein 1-aminocyclobutane carboxylic acid replaced 1-aminocyclohexanecarboxylic acid. 1H NMR (400 MHz, de-DMSO): 8.08 (dd, IH), 7.71 (m, 2H), 7.25 (br s, 3H), 2.70 (m, 2H), 2.15 (m, 2H), 2.06 (m, IH), 1.94 (m, IH). MS (EI) for Ci4Hi2BrN3O2: 334 (MH+). [00840] Compounds disclosed are active againt either PIM (wherein PIM refers to PIM- 1 , PIM-2, and/or PIM-3 throughout this application), CDC7, or CK2 kinase activity (as defined by Activity A, Activity B, and Activity C). Accordingly, compounds of this dislcosure can also be useful for treating proliferative disorders associated with PIM, CDC7 or CK2 kinase activity. Characterization of CDC7 Inhibition in a Biochemical Chemiluminesence Assay (IC50 determination):
[00841] The IC50 evaluation of compounds against the CDC7 enzyme was performed in a Chemiluminesence assay utilizing a Greiner 384-well white binding plate format. Generally, 0.5 μL DMSO containing varying concentrations of the test compound was mixed with 15 μL buffer solution consisting of 50 mM Hepes (pH = 7.5), 10 mM MgCl2, 0.02% BSA, 0.02% Tween, 0.02% Brij-35 and ImM DTT.
Upon addition of 10 μL of CDC-7 (GB) to the designated well plates and subsequent incubation for ten minutes at ambient temperature, 10 μL of ATP mix in Tris Base was added. Following an incubation period of 90 minutes at ambient temperature (24-27 0C), 20 μL of Kinase GIo (Promega) was added to measure kinase activity by quantitating the amount of ATP remaining in solution. The luminescence was measured following a Luciferase Protocol on either En Vision or Victor plate readers. Characterization of PIM-I Inhibition in a Biochemical Chemiluminesence Assay (IC50 determination):
[00842] The IC50 evaluation of compounds against the PIM-I oncogene was performed in a Chemiluminesence assay utilizing a Greiner 384-well white medium binding white plate format. Generally, 0.5 μL DMSO containing varying concentrations of the test compound was mixed with stock buffer solution consisting of 20 mL of IM Hepes (pH = 7.4), 10 mL of IM MgCl2, 3 mL of 10% Triton X-IOO and 68mL of Milli-Q water followed by subsequent addition of ImM DTT (Dithiotheritol). The assay procedure involves compound dilution followed by addition of 4.6 nM PIM-I to the designated well plates and subsequent pre -incubation of the compounds with the enzyme for thirty minutes at ambient temperature (24-27 0C). 10 μL of PIM-I substrate/ATP mix (4nM AKRRRLSA substrate sequence/ lμM ATP) was added twice. Following an incubation period of 120 minutes at ambient temperature (24-27 0C), 10 μL of Kinase GIo (Promega) was added. The luminescence was measured following a Luminescence Protocol on a Victor plate reader. Final assay conditions required 2.3 nM PIM-I (protooncogene-encoded protein Kinase PIM-I substrate), 0.5 μM ATP, 10 μM AKRRRLSA sequence, 20.5 μL reaction volume, 2.5% final concentration in DMSO with a solvent to buffer ratio of 2.5. Characterization of PIM-2 Inhibition in a Biochemical Chemiluminesence Assay (IC50 determination):
[00843] The IC50 evaluation of compounds against the PIM-2 oncogene was performed in a Chemiluminesence assay utilizing a Greiner 384-well white medium binding white plate format. Generally, 0.5 μL DMSO containing varying concentrations of the test compound was mixed with stock buffer solution consisting of 20 mL of IM Hepes (pH = 7.4), 10 mL of IM MgCl2, 3 mL of 10% Triton X-100 and 68 mL of Milli-Q water followed by subsequent addition of ImM DTT. The assay procedure involves compound dilution followed by addition of 3 nM PIM-2 to the designated well plates and subsequent pre-incubation of the compounds with the enzyme for 30 minutes at ambient temperature (24-27 0C). Thereafter, 10 μL of PIM substrate/ATP mix (4nM AKRRRLSA substrate sequence/ lμM ATP) was added twice. Following an incubation period of 120 minutes at ambient temperature (24-27 0C), 10 μL of Kinase GIo (Promega) was added. The luminescence was measured following a Luminescence Protocol on a Victor plate reader. Final assay conditions required 1.5 nM PIM-2, 0.5 μM ATP, 10 μM AKRRRLSA sequence, 20.5 μL reaction volume, 2.5% final concentration in DMSO with a solvent to buffer ratio of 2.0. Characterization of PIM-3 Inhibition in a Biochemical Chemiluminesence Assay (IC50 determination):
[00844] The IC50 evaluation of compounds against the PIM-3 Oncogene was performed in a Chemiluminesence assay utilizing a Greiner 384-well white medium binding white plate format. Generally, 0.5 μL DMSO containing varying concentrations of the test compound was mixed with stock buffer solution consisting of 20 mL of IM Hepes (pH = 7.4), 10 mL of IM MgCl2, 3 mL of 10% Triton X-100 and 68 mL of Milli-Q water followed by subsequent addition of ImM DTT. The assay procedure involves the compound dilution followed by addition of 1.6 nM PIM- 3 to the designated well plates and subsequent pre-incubation of the compounds with the enzyme for 30 minutes at ambient temperature (24-27 0C). Thereafter, 10 μL of PIM substrate/ATP mix (4nM AKRRRLSA substrate sequence/ lμM ATP) was added twice. Following an incubation period of 120 minutes at ambient temperature (24-27 0C), 10 μL of Kinase GIo (Promega) was added. The luminescence was measured following a Luminescence Protocol on a Victor plate reader. Final assay conditions required 0.8 nM PIM-3, 0.5 μM ATP, 10 μM AKRRRLSA sequence, 20.5 μL reaction volume, 2.5% final concentration in DMSO with a solvent to buffer ratio of 2.5
Characterization of CK2 Inhibition in a Biochemical Chemiluminesence Assay (IC50 determination): [00845] The IC50 evaluation of compounds against CK2 was performed in a Chemiluminesence Holoenzyme assay utilizing a Greiner 384-well white medium binding white plate format. Generally, 0.5 μL DMSO containing varying concentrations of the test compound was mixed with stock buffer solution consisting of IM Tris base (pH = 7.5), 1OmM MgCl2, 0.03% Triton X-100 followed by subsequent addition of ImM DTT and 0. lmMNaV03. The assay procedure involves the compound dilution scheme followed by addition of 10 μL of the substrate twice, followed by 10 μL twice of the respective enzyme or buffer to the designated well plates and subsequent pre -incubation of the compounds. Following an incubation period of 150 minutes at ambient temperature (24-27 0C), 10 μL of Kinase GIo (Promega) was added. The luminescence was measured following a Luminescence Protocol on a Victor plate reader. The substrate stock solution requires 1OmM ATP and ImM of Casein and the enzyme stock solution requires 12.3uM of the holoenzyme at a 1OnM final concentration. [00846] From the foregoing it will be appreciated that, although specific embodiments of this disclosure have been described herein for purposes of illustration, various modifications may be made without deviating from the spirit and scope of the invention. Accordingly, the invention is not limited except as by the appended claims.

Claims

What is claimed is:
1. A compound according to formula I:
Figure imgf000368_0001
or a pharmaceutically acceptable salt thereof; wherein: R1 is hydrogen or alkyl;
R2 is selected from aminocarbonylalkylaminoalkyl, aminoalkylaminoalkyl, dialkylaminoalkylaminoalkyl, carboxyalkylaminoalkyl, cycloakylaminoalkyl, dialkylaminoarylalkylaminoalkyl, heteroarylalkylaminoalkyl, arylalkyl optionally substituted at any aryl or alkyl position with 1-3 groups selected from halo and -NH2, heterocycloalkylarylalkylaminoalkyl optionally substituted at the heterocycloalkyl portion with alkyl, aminoalkyl optionally substituted with 1 , 2 or 3 -OH, alkylamino optionally substituted with 1 , 2 or 3 -OH, alkylaminoalkyl optionally substituted with 1 , 2 or 3 -OH, alkoxyalkylaminoalkyl, heterocycloalkylaminoalkyl optionally substituted with alkyl at the heterocycloalkyl portion, hydroxyalkyl, cycloalkylaminoalkyl, arylamino(alkyl)alkyl optionally substituted at any ring position with 1, 2 or 3 halo, heterocycloalkylalkylaminoalkyl optionally substituted at any ring position with 1-2 alkyl, arylalkylaminoalkyl optionally substituted at the aryl position with dialkylamino, halo, alkoxy, heteroaryl, or alkylheterocycloalkyl, dialkylaminoalkyl optionally substituted with 1 , 2 or 3 -OH, arylaminoalkyl optionally substituted at the aryl portion with 1, 2 or 3 groups selected from halo, heterocycloalkyl, alkylheterocycloalkyl and dialkylamino, heteroarylaminoalkyl, arylamino, aryloxyalkyl, unsubstituted heteroarylalkyl, heteroarylalkyl substituted at any alkyl position with alkyl, aryl, arylalkyl or amino, -(C4-Cy)cycloalkyl optionally substituted with -NH2, -NHC(O)-O-(CH3)3, or aminoalkyl, -N(H)C(O)-O-alkyl, alkylpiperazinylcarbonyl, alkylheterocycloalkylalkoxyalkyl, alkylheteroarylalkoxyalkyl,heteroaryl optionally substituted at any ring position with 1, 2 or 3 substituents selected from amino, alkyl, alkylamino, halo, -O- heterocycloalkyl, alkoxy, aminoalkyl, dialkylaminoalkylamino, heterocycloalkylalkylamino and heterocycloalkyl, heterocycloalkylalkylamino, heterocycloalkylalkyl optionally substituted with 1 , 2 or 3 R4 groups at any ring position, aryl substituted with 1, 2 or 3 R5 groups at any ring position, -NHC(O)R7, aminoalkylamino, -CR11R12, heterocycloalkyl optionally substituted with 1, 2 or 3 R10 groups, heteroarylalkyl substituted at any ring position with 1, 2 or 3 alkyl, halo, aryl or arylalkyl groups, heteroarylamino, heterocycloalkylalkoxyalkyl, dialkylaminoalkylamino, heterocycloalkylamino, carboxyalkyl, arylalkylamino optionally substituted with heterocycloalkyl or heterocycloalkylalkyl, and heterocycloalkyloxyalkyl; or R1 and R2, together with the carbon atoms to which they are attached, join to form a five membered heterocycloalkyl ring;
R3a is selected from halo, alkyl, -NO2, alkoxy, alkynyl optionally substituted with R14, alkoxycarbonylalkyl, arylalkoxy, -C(O)N(H)alkyl, -N(H)-C(O)-alkyl, - C(O)-alkyl, -CN, phenyl, -OCF3, -N(H)R13, -OH, -CF3, -S-CH3 and hydroxymethylalkynyl; R3b, R3c and R3d are each independently selected from H, -OH, -N+(O)OH, alkoxyl, and halo; or R3a is hydrogen and R3b, R3c and R3d are each independently selected from -CF3, -OH, alkoxy, and halo; or R3a and R3d, together with the carbons to which they are attached, join to form a 5 membered heteroaryl optionally substituted with methyl or -NH2, or a 5-6 membered heterocycloalkyl;
R4 is selected from -OH, amino, aminoalkyl, halo, alkyl optionally substituted with -OH, alkoxy, alkylaminoalkyl, heteroarylalkyl, -C(O)OH, -C(O)-O-alkyl, -C(O)- alkyl, oxo, aryl optionally substituted with alkyl, arylalkyl or halo, heteroaryl, -OH, dialkylamino, dialkylaminoalkyl, alkylamino, spiro-heterocycloalkyl, -NHC(O)R8, -C(O)NHR9, arylalkylaminocarbonyl optionally substituted with halo at any ring position of the aryl, heterocycloalkylalkylamino, dialkylaminoalkylcarbonyl, dialkylaminocarbonylalkyl, heterocycloalkylalkyl optionally substituted with -CF3, heterocycloalkyl optionally substituted with alkyl, arylalkyl optionally substituted with
-CF3, alkoxyalkyl and heterocycloalkylcarbonyl optionally substituted with -OH or halo; R5 is selected from alkyl, -OH, amino, aminoalkyl, -C(O)N(H)- heteroarylalkyl, halo, -NO2, -C(O)-N(H)-heterocycloalkylalkyl, alkylaminoalkyl, heteroaryl, cycloalkylaminoalkyl, alkylamino, dialkylamino, -C(O)Oalkyl, -C(O)OH, heterocycloalkyl, -N(H)-alkylheterocycloalkylC(O)-O-alkyl, -O-alkyl-C(O)-N(H)- alkylcycloalkyl, -C(O)-N(H)-alkyl, -C(O)N(H)alkylaryl, -C(O)N(H)-cycloalkyl, alkylthio, alkylsulfonyl, -O-alkylheterocycloalkyl, heteroarylalkylamino, -CF3, heterocycloalkylalkylamino optionally substituted with alkyl at any ring position, alkylsulfonyl, -NHC(O)R6, alkoxycarbonylheterocycloalkylaminoalkyl, heterocycloalkylaminoalkyl optionally substituted at the heterocycloalkyl portion with alkoxycarbonyl, dialkylaminoalkyl, dialkylaminoalkylamino, and alkoxy;
R6 is selected from dialkylaminoalkyl, heteroarylamino, heterocycloalkyl, heterocycloalkylalkyl optionally substituted with -OH, cycloalkyl, heteroarylalkyl, alkoxyalkyl, heterocycloalkyl, heteroaryl optionally substituted with 1 , 2 or 3 groups selected from halo, -NH2, aminoalkylaminocarbonyl, heteroaryl, hydroxyalkyl, alkoxy, alkyl, -C(O)-O-alkyl and -C(O)-O-H, alkyl, alkoxy, and aryl optionally substituted 1, 2 or 3 halo, -N(H)C(O)CH3, alkyl or alkoxy;
R7 is selected from heterocycloalkylalkyl, arylalkyl optionally substituted at any ring position with 1, 2 or 3 halo groups, dialkylaminoalkyl, heterocycloalkyl, heteroaryl optionally substituted at any ring position with 1 , 2 or 3 groups selected from halo and
-COOH, and alkoxyalkyl;
R8 is selected from arylalkyl, heterocycloalkyl and alkyl;
R9 is selected from H, alkyl, arylalkyl optionally substituted with halo at any ring position of the aryl, heterocycloalkyl, arylalkylaminocarbonyl optionally substituted with halo and dialkylaminoalkyl;
R10 is selected from alkyl, oxo, heteroaryl, dialkylaminoalkylcarbonyl, dialkylaminocarbonylalkyl, aminoalkyl, -OH, halo, heteroarylcarbonyl, dialkylaminoalkyl, heterocycloalkyl (piperidinyl) optionally substituted with alkyl, -C(O)-O-alkyl, arylalkylcarbonyl, arylcarbonyl, alkylcarbonyl, alkoxyalkylcarbonyl, heterocycloalkylcarbonyl, heteroarylalkyl, -O-heterocycloalkyl and arylalkyl;
R11 is selected from aryl optionally substituted with halo, heteroarylalkyl, cycloalkyl, spiro-cycloalkyl and arylalkyl optionally substituted with alkoxy or phenylmethylmethoxy; R12 is selected from -NH2 and heterocycloalkyl optionally substituted with alkyl;
R13 is selected from arylalkyl wherein the aryl portion of arylalkyl is optionally substituted with 1, 2 or 3 alkoxy, halo, methyl, methoxy, -CF3, cycloalkyl, and heteroarylalkyl; and
R14 is selected from hydroxylalkyl, H and TMS.
2. The compound according to claim 1, or a pharmaceutically acceptable salt thereof, wherein R2 is selected from -(Ci-C3)alkyl-phenyl optionally substituted with 1-3 halo, -NH-phenyl, -NH-piperidinyl, -NH-pyridinyl, -NH(Ci-C3)alkylphenyl optionally substituted at any phenyl position with piperazinyl or methylpiperazinyl, - NH(Ci-C3)alkyl-N(CH3)2, -NH(Ci-C3)alkyl-OH, phenyl substituted with 1, 2 or 3 Xa, phenyl substituted with 0-2 Xb groups and 1 Xc, methylpiperazinylphenylalkoxyalkyl (methylpiperazinylphenylmethoxymethyl), methylpiperazinylcarbonyl, 2- chlorophenyl-4-methylpiperazinylmethyl, (4-methylpiperazin- 1 -yl)(phenyl)methyl, 1 - (4-methylpiperazin- 1 -yl)-2-phenylethyl, 2-chlorophenyl(4-methylpiperazin- 1 - yl)methyl, 4-oxo-3-phenyl-l,3,8-triazaspiro[4.5]dec-l-yl)methyl, -(Ci- C3)alkylC(O)OH, hydroxyalkyl, -(Ci-C3)alkyl-N(Rza)-aryl optionally substituted with chloro, fluoro, piperazinyl, methylpiperazinyl and dialkylamino, -(5-10)membered heteroaryl optionally substituted with 1 or 2 groups selected from halo, -(Ci-Ce)alkyl, -(Ci-Ce)alkoxy, piperidinylalkylamino, piperdinylalkylamino, alkylamino, aminoalkyl, dialkylaminoalkylamino, piperidinyloxy, piperidinyl, and amino, -(Ci- C3)alkyl-O-phenyl, -(Ci-C3)alkyl-O-(Ci-C3)alkyl-(5-6 membered)heterocycloalkyl, - (Ci-C3)alkyl-N(H)-heteroaryl, -(Ci-C3)alkyl-(5-10)membered heteroaryl optionally substituted with -(Ci-C3)alkyl, halo or phenyl, oxopyrrolidinyl optionally substituted with OH or piperidinyl,
-(Ci-C4)alkyl-(3-9 membered)heterocycloalkyl optionally substituted at the (3-9 membered)heterocycloalkyl with Xd, -(Ci-C6)alkyl-NRzb-(Ci-C4)alkyl wherein the - (Ci-C4)alkyl portion is optionally substituted with Xe, -(Ci-C3)alkyl-NH-(C3- C6)cycloalkyl, -(CH2)-NH-(C3-C6)cyclohexyl, -(Ci-C3)alkyl-NH2, wherein the -(Ci-C3)alkyl- portion of -(Ci-C3)alkyl-NH2 is optionally substituted with Xf, -(3-9 membered)heterocycloalkyl optionally substituted with Xg, -(C3-Ce)-cycloalkyl (cyclohexyl) optionally substituted with amino, -NHC(O)-O-(CH3)3, aminoalkyl and dialkylaminoalkylamino;
Xa is selected from halo, phenyl substituted with a group selected from -
COOH, -COOCH3, NO2, -(Ci-C3)alkoxy, methylthio, -(Ci-C3)alkyl, -NH2, -OH, -Nt(C1-
C3)alkyl]2, -CF3 and methylsulfonyl;
Xb, when present, is independently selected from alkyl, -NH2 and halo; Xc is selected from -(5-6 membered)heteroaryl (imidazole), -(5-6 membered)heterocycloalkyl (piperazinyl), alkylcarbonylamino, alkylaminocarbonyl, cycloalkylaminoalkyl (cyclohexylaminoalkyl), -NH(Ci-C3)alkyl-(3-6 membered)heterocycloalkyl, dimethylamino-(Ci-C3)alkylcarbonylamino, cycloalkylaminocarbonyl, dialkylaminoalkylcarbonylamino, cycloalkylmethylaminocarbonylmethyloxy, phenylalkylaminocarbonyl, heterocycloalkylaminoalkyl optionally substituted with alkoxycarbonyl, dialkylaminoalkyl, morpholinylalkoxy, alkylaminoalkyl, dialkylaminoalkylamino, alkoxycarbonylheterocycloalkylalkylamino, heterocycloalkylalkylamino optionally substituted with methyl, heterocycloalkylalkylcarbonylamino optionally substituted with -OH, , heterocycloalkylcarbonylamino optionally substituted with 1 or 2 groups selected from halo and methyl, heteroarylcarbonylamino optionally substituted with 1 or 2 groups selected from amino, alkyl, halo, -C(O)OH, pyrazolyl, -OCH3 and -
C(O)OCH3,
-N(H)C (O)phenyl optionally substituted with 1 or 2 groups selected from halo, methyl, methoxy and -NHC(O)CH3, -N(H)C(O)alkyl, -N(H)C(O)(C1-
C3)alkylpyridinyl, -N(H)C(O)(Ci-C3)alkylpiperidinyl, -N(H)C(O)-lH-pyrrolo[2,3-b]pyridinyl,
-N(H)C(O)cyclohexyl, N(H)C(O)cyclopentyl, -N(H)C(O)(C i-C3)alkylmorpholinyl,
-N(H)C(O)(Ci-C3)alkylpyridinyl, -N(H)C(O)(Ci-C3)alkylimidazolyl, aminoalkyl, and
-N(H)C(O)N(H)pyrimidinyl;
Xd is selected from alkyl, 1-3 halo, -COOH, phenyl optionally substituted with 1 or 2 groups selected from halo, methyl and methylphenyl, phenylmethyl, spiro- piperidine, trifluoromethylphenylmethyl, -(Ci-C3)alkoxy, pyridinyl, dimethylaminoalkyl, dimethylamino, hydroxylalkyl, dimethylaminoalkylaminocarbonyl, alkylamino, aminoalkyl, dimethylaminocarbonylalkyl, diethylaminoalkylcarbonyl, -(C i -C3)alkyl-(5 -6 membered)heterocycloalkyl, (5-6 membered)heterocycloalkyl optionally substituted with -(Ci-C3)alkyl, -NH2, -OH, heterocycloalkylalkylamino, alkoxyalkyl, -C(O)CH3, -C(O)NH(C i-C3)alkylphenyl optionally substituted with 1-3 halo at any phenyl position, one -OH and one -C(O)NH(C i-C3)alkylphenyl optionally substituted with 1-3 halo at any phenyl position, -C(O)-heterocycloalkyl optionally substituted with
-OH or halo, alkoxycarbonyl, aminocarbonyl, one -OH and one methyl, one -OH and one -C(O)OH, one -OH and one -NHC(O)piperidinyl, one -OH and one alkyl; Xe is selected from dialkylamino, amino, 1-3 -OH, alkoxy, A- methylpiperazinylphenyl, dimethylaminophenyl, phenyl optionally substituted with 1- 3 groups selected from halo and methoxy, heteroaryl, -(Ci-C3)alkylC(O)NH2, - C(O)NH2,
-C(O)OH, -(Ci-C3)alkylC(O)OH, and heterocycloalkyl optionally substituted with 1- 2 alkyl;
Xf is selected from cycloalkyl, spirocycloalkyl, phenyl, phenylalkyl optionally substituted with phenylmethyloxy or alkoxy, and thienylalkyl;
Xg is selected from alkyl, alkylcarbonyl, heterocycloalkylcarbonyl, dialkylaminoalkylcarbonyl, 1 -methylpiperidinyl, dialkylaminoalkyl, heteroarylcarbonyl, alkoxyalkylcarbonyl, phenylcarbonyl, phenylalkylcarbonyl, oxo, phenylalkyl, -(5-6 membered)heteroarylalkyl, piperidinyloxy, -OH, oxo, 1-2 halo and 1-2 methyl;
Rza is H or methyl; and
Rzb is H or alkyl optionally substituted with 1-3 -OH.
3. The compound according to claim 1, or a pharmaceutically acceptable salt thereof, wherein:
R1 is hydrogen or alkyl;
R2 is selected from aminocarbonylalkylaminoalkyl, aminoalkylaminoalkyl, dialkylaminoalkylaminoalkyl, carboxyalkylaminoalkyl, cycloakylaminoalkyl, dialkylaminoarylalkylaminoalkyl, heteroarylalkylaminoalkyl, arylalkyl, heterocycloalkylarylalkylaminoalkyl optionally substituted at the heterocycloalkyl portion with alkyl, alkoxyalkylaminoalkyl, heterocycloalkylaminoalkyl optionally substituted with alkyl at the heterocycloalkyl portion, hydroxyalkyl, cycloalkylaminoalkyl, arylamino(alkyl)alkyl optionally substituted at any ring position with 1, 2 or 3 halo, heterocycloalkylalkylaminoalkyl optionally substituted at any ring position with 1-2 alkyl, arylalkylaminoalkyl optionally substituted at the aryl position with dialkylamino, halo, alkoxy, heteroaryl, or alkylheterocycloalkyl, arylamino alkyl optionally substituted at the aryl portion with 1, 2 or 3 groups selected from halo, alkylheterocycloalkyl and dialkylamino, heteroarylaminoalkyl, arylamino, aryloxyalkyl, heteroarylalkyl optionally substituted with arylalkyl, alkyl or aryl, - N(H)C(O)-O-alkyl, alkylpiperazinylcarbonyl, alkylheterocycloalkylalkoxyalkyl, alkylheteroarylalkoxyalkyl, heteroaryl optionally substituted at any ring position with 1, 2 or 3 substituents selected from -O-heterocycloalkyl, dialkylaminoalkylamino and heterocycloalkylalkylamino, heterocycloalkylalkylamino, heterocycloalkylalkyl optionally substituted with 1 , 2 or 3 R4 groups at any ring position, aryl substituted with 1, 2 or 3 R5 groups at any ring position, -NHC(O)R7, aminoalkylamino, - CR11R12, heterocycloalkyl optionally substituted with 1, 2 or 3 R10 groups, heteroarylalkyl optionally substituted at any ring position with 1, 2 or 3 alkyl or aryl groups, heteroarylamino, heterocycloalkylalkoxyalkyl, dialkylaminoalkylamino, heterocycloalkylamino, carboxyalkyl, and heterocycloalkyloxyalkyl; or R1 and R2, together with the carbon atoms to which they are attached, join to form a five membered heterocycloalkyl ring; R3a is selected from halo, alkyl, -NO2, alkoxy, alkynyl optionally substituted with R14, alkoxycarbonylalkyl, arylalkoxy, -C(O)N(H)alkyl, -N(H)-C(O)-alkyl, - C(O)-alkyl, -CN, phenyl, -OCF3, -N(H)R13, -OH, -CF3, -S-CH3 and hydroxymethylalkynyl;
R3b, R3c and R3d are each independently selected from H, -OH, -N+(O)OH, alkoxyl, and halo; or R3a is hydrogen and R3b, R3c and R3d are each independently selected from -CF3, -OH, alkoxy, and halo; or R3a and R3d, together with the carbons to which they are attached, join to form a 5 membered heteroaryl optionally substituted with methyl or -NH2, or a 5-6 membered heterocycloalkyl;
R4 is selected from -OH, amino, aminoalkyl, halo, alkyl optionally substituted with -OH, alkoxy, alkylaminoalkyl, heteroarylalkyl, -C(O)OH, -C(O)-O-alkyl, -C(O)- alkyl, oxo, aryl optionally substituted with alkyl or halo, heteroaryl, -OH, dialkylamino, dialkylaminoalkyl, alkylamino, spiro-heterocycloalkyl, -NHC(O)R8, -C(O)NHR9, arylalkylaminocarbonyl optionally substituted with halo at any ring position of the aryl, heterocycloalkylalkylamino, dialkylaminoalkylcarbonyl, dialkylaminocarbonylalkyl, heterocycloalkyl alkyl optionally substituted with -CF3, heterocycloalkyl optionally substituted with alkyl, alkoxyalkyl, arylalkyl optionally substituted with -CF3, and heterocycloalkylcarbonyl optionally substituted with -OH or halo;
R5 is selected from -C(O)N(H)-heteroarylalkyl, -C(O)-N(H)- heterocycloalkylalkyl, alkylaminoalkyl, cycloalkylaminoalkyl,
-N(H)-alkylheterocycloalkylC(O)-O-alkyl, -O-alkyl-C(O)-N(H)-alkylcycloalkyl, -C(O)N(H)alkylaryl, -C(O)N(H)-cycloalkyl, -O-alkylheterocycloalkyl, heteroarylalkylamino, heterocycloalkylalkylamino optionally substituted with alkyl at any ring position, alkoxycarbonylheterocycloalkylaminoalkyl, heterocycloalkylaminoalkyl optionally substituted at the heterocycloalkyl portion with alkoxycarbonyl, dialkylaminoalkyl and dialkylaminoalkylamino;
R6 is selected from dialkylaminoalkyl, heteroarylamino, heterocycloalkyl, heterocycloalkylalkyl optionally substituted with -OH, cycloalkyl, heterocycloalkylalkyl, heteroarylalkyl, alkoxyalkyl, heterocycloalkyl, heteroaryl optionally substituted with 1, 2 or 3 groups selected from halo, -NH2, aminoalkylaminocarbonyl, heteroaryl, hydroxyalkyl, alkoxy, alkyl, -C(O)-O-alkyl and -C(O)-O-H, alkyl, alkoxy, and aryl optionally substituted 1, 2 or 3 halo, - N(H)C(O)CH3, alkyl or alkoxy;
R7 is selected from heterocycloalkylalkyl, arylalkyl optionally substituted at any ring position with 1, 2 or 3 halo groups, dialkylaminoalkyl, heterocycloalkyl, heteroaryl optionally substituted at any ring position with 1 , 2 or 3 groups selected from halo and -COOH, and alkoxyalkyl;
R8 is selected from arylalkyl, heterocycloalkyl and alkyl; R9 is selected from H, alkyl, arylalkyl optionally substituted with halo at any ring position of the aryl, heterocycloalkyl, arylalkylaminocarbonyl optionally substituted with halo and dialkylaminoalkyl; R10 is selected from alkyl, oxo, heteroaryl, dialkylaminoalkylcarbonyl, dialkylaminocarbonylalkyl, aminoalkyl, -OH, halo, heteroarylcarbonyl, dialkylaminoalkyl, heterocycloalkyl (piperidinyl) optionally substituted with alkyl, -C(O)-O-alkyl, arylalkylcarbonyl, arylcarbonyl, alkylcarbonyl, alkoxyalkylcarbonyl, heterocycloalkylcarbonyl, heteroarylalkyl, -O-heterocycloalkyl and arylalkyl optionally substituted with alkoxy or arylalkoxy;
R11 is selected from aryl optionally substituted with halo, heteroarylalkyl, cycloalkyl, spiro-cycloalkyl and arylalkyl optionally substituted with alkoxy or phenylmethylmethoxy; R12 is selected from -NH2 and heterocycloalkyl optionally substituted with alkyl;
R13 is selected from arylalkyl wherein the aryl portion of arylalkyl is optionally substituted with 1, 2 or 3 alkoxy, halo, methyl, methoxy, -CF3, cycloalkyl (cyclohexyl), and heteroarylalkyl; and R14 is selected from hydroxylalkyl, H and TMS .
4. The compound according to claim 1, or a pharmaceutically acceptable salt thereof, wherein R2 is selected -NH-phenyl, -NH-piperidinyl, -NH-pyridinyl, -NH(Ci- C3)alkylphenyl optionally substituted at any phenyl position with piperazinyl or methylpiperazinyl, -NH(Ci-C3)alkyl-N(CH3)2, -NH(Ci-C3)alkyl-OH, -(Ci-C3)alkyl-O- phenyl, -(Ci-C3)alkyl-O-(Ci-C3)alkyl-(5-6 membered)heterocycloalkyl, -(C1- C3)alkyl-N(H)-heteroaryl, -(Ci-C3)alkyl-(5-10)membered heteroaryl optionally substituted with
-(Ci-C3)alkyl, halo or phenyl, oxopyrrolidinyl optionally substituted with OH or piperidinyl, -(Ci-C4)alkyl-(3-9 membered)heterocycloalkyl optionally substituted at the (3-9 membered)heterocycloalkyl with Xd, -(C1-C6)alkyl-NRzb-(C1-C4)alkyl wherein the
-(Ci-C4)alkyl portion is substituted with Xe, -(Ci-C3)alkyl-NH-(C3-C6)cycloalkyl, -(CH2)-NH-(C3-C6)cyclohexyl, -(Ci-C3)alkyl-NH2, wherein the -(Ci-C3)alkyl- portion of -(Ci-C3)alkyl-NH2 is substituted with Xf, and -(3-9 membered)heterocycloalkyl optionally substituted with Xg;
Xd is selected from alkyl, 1-3 halo, -COOH, phenyl optionally substituted with 1 or 2 groups selected from halo, methyl and methylphenyl, phenylmethyl, spiro- piperidine, trifluoromethylphenylmethyl, -(Ci-C3)alkoxy, pyridinyl, dimethylaminoalkyl, dimethylamino, hydroxylalkyl, dimethylaminoalkylaminocarbonyl, alkylamino, aminoalkyl, dimethylaminocarbonylalkyl, diethylaminoalkylcarbonyl, -(C i -C3)alkyl-(5 -6 membered)heterocycloalkyl, (5-6 membered)heterocycloalkyl optionally substituted with -(Ci-C3)alkyl, -NH2, -OH, heterocycloalkylalkylamino, alkoxyalkyl,
-C(O)CH3, -C(O)NH(C i-C3)alkylphenyl optionally substituted with 1-3 halo at any phenyl position, one -OH and one -C(O)NH(C i-C3)alkylphenyl optionally substituted with 1-3 halo at any phenyl position, -C(O)-heterocycloalkyl optionally substituted with -OH or halo, alkoxycarbonyl, aminocarbonyl, one -OH and one methyl, one -OH and one -C(O)OH, one -OH and one -NHC(O)piperidinyl, one -OH and one alkyl;
Xe is selected from dialkylamino, amino, 1-3 -OH, alkoxy, A- methylpiperazinylphenyl, dimethylaminophenyl, phenyl optionally substituted with 1- 3 groups selected from halo and methoxy, heteroaryl, -(Ci-C3)alkylC(O)NH2, - C(O)NH2,
-C(O)OH, -(Ci-C3)alkylC(O)OH, and heterocycloalkyl optionally substituted with 1- 2 alkyl;
Xf is selected from cycloalkyl, spirocycloalkyl, phenyl, phenylalkyl optionally substituted with phenylmethyloxy or alkoxy, and thienylalkyl; Xg is selected from alkyl, alkylcarbonyl, heterocycloalkylcarbonyl, dialkylaminoalkylcarbonyl, 1 -methylpiperidinyl, dialkylaminoalkyl, heteroarylcarbonyl, alkoxyalkylcarbonyl, phenylcarbonyl, phenylalkylcarbonyl, oxo, phenylalkyl, -(5-6 membered)heteroarylalkyl, piperidinyloxy, -OH, oxo, 1-2 halo and 1-2 methyl; and Rzb is H or alkyl optionally substituted with 1-3 -OH.
5. The compound according to claim 1, or a pharmaceutically acceptable salt thereof, wherein R3a is selected from halo, alkyl, -NO2, alkoxy, alkynyl optionally substituted with R14, alkoxycarbonylalkyl, arylalkoxy, -C(O)N(H)alkyl, -N(H)-C(O)- alkyl, -C(O)-alkyl, -CN, phenyl, -OCF3, -N(H)R13, -OH, -CF3, -S-CH3 and hydroxymethylalkynyl; and
R3b, R3c and R3d are each H.
6. The compound according to claim 1, or a pharmaceutically acceptable salt thereof, wherein R3a is halo, alkoxy or -OCF3; and R3b, R3c and R3d are each H.
7. The compound according to claim 1, or a pharmaceutically acceptable salt thereof, wherein R2 is a heterocycloalkyl selected from azetidinyl, pyrrolidinyl, piperazinyl and piperidinyl optionally substituted with 1, 2 or 3 R10; R3a is halo, alkoxy or -OCF3; and R3b, R3c and R3d are each H.
8. The compound according to claim 1, or a pharmaceutically acceptable salt thereof, wherein R2 is a heteroaryl optionally substituted at any ring position with 1 , 2 or 3 substituents selected from amino, alkylamino, halo, -O-heterocycloalkyl, alkoxy, aminoalkyl, dialkylaminoalkylamino, heterocycloalkylalkylamino and heterocycloalkyl; R3a is halo, alkoxy or -OCF3; and R3b, R3c and R3d are each H.
9. The compound according to claim 1, or a pharmaceutically acceptable salt thereof, wherein R2 is a heterocycloalkylalkyl optionally substituted with 1 , 2 or 3 R4 groups at any ring position; R3a is halo, alkoxy or -OCF3; and R3b, R3c and R3d are each H.
10. The compound according to claim 1, or a pharmaceutically acceptable salt thereof, wherein R2 is an aryl substituted with 1 , 2 or 3 R5 groups at any ring position; R3a is halo, alkoxy or -OCF3; and R3b, R3c and R3d are each H.
11. The compound according to claim 1 , or a pharmaceutically acceptable salt thereof, wherein R2 is a heterocycloalkyl optionally substituted with 1, 2 or 3 R10 groups; R3a is halo, alkoxy or -OCF3; and R3b, R3c and R3d are each H.
12. The compound according to claim 1, or a pharmaceutically acceptable salt thereof, wherein R2 is heterocycloalkylalkyl, and wherein the heterocycloalkyl portion of the heterocycloalkylalkylalkyl is pyrrolidinyl, piperadinyl, piperazinyl, azetidinyl or a 7 membered bridged heterocyclic ring optionally substituted with hydroxyl; 13. The compound according to claim 1, or a pharmaceutically acceptable salt thereof, wherein R2 is a heteroarylalkyl optionally substituted at any ring position with 1, 2 or 3 alkyl or aryl groups; R3a is halo, alkoxy or
-OCF3; and R3b, R3c and R3d are each H.
14. The compound according to claim 1, or a pharmaceutically acceptable salt thereof, wherein R2 is
Figure imgf000379_0001
wherein Ri 5 is selected from H or -(Ci -Ce)alkyl, Ri6 is selected from H, phenyl and -(Ci-C6)alkyl, and Rn is selected from H, -(Ci-C3)alkylC(O)NH2, -(Ci-C3)alkylC(O)OH and heterocycloalkylalkyl
15. The compound according to claim 1 selected from one of the following compounds:
8-bromo-2-(pyrrolidin-l-ylmethyl)[l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
8-bromo-2-(piperidin- 1 -ylmethyl)[ 1 ]benzofuro[3 ,2-d]pyrimidin-4(3H)-one;
8-bromo-2-[(4-methylpiperazin- 1 -yl)methyl] [ 1 ]benzofuro[3 ,2-d]pyrimidin-4(3H)-one;
8-bromo-2-[( { [4-(4-methylpiperazin- 1 - yl)phenyl]methyl} amino)methyl] [ 1 Jbenzofuro [3 ,2-d]pyrimidin-4(3 H)-one;
8-chloro-2-(pyrrolidin- 1 -ylmethyl)[ 1 ]benzofuro[3,2-d]pyrimidin-4(3H)-one;
8-bromo-2-( 1 H-imidazol- 1 -ylmethyl)[ 1 ]benzofuro[3 ,2-d]pyrimidin-4(3H)-one;
8-chloro-2-[(4-methylpiperazin-l-yl)methyl][l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
8-bromo-2-[(piperidin-4-ylamino)methyl][l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
8-bromo-2-({4-[3-(dimethylamino)propyl]piperazin-l-yl}methyl)[l]benzofuro[3,2- d]pyrimidin-4(3H)-one;
8-bromo-2-[( { [2-(dimethylamino)phenyl]methyl} amino)methyl] [ 1 ]benzofuro[3 ,2- d]pyrimidin-4(3H)-one;
8-bromo-2- { [(2-chlorophenyl)amino]methyl} [ 1 ]benzofuro[3 ,2-d]pyrimidin-4(3H)-one;
8-bromo-2-{[(3-fluorophenyl)amino]methyl}[l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
8-bromo-2-[(pyridin-3-ylamino)methyl][l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
8-bromo-2-[( { [3-(4-methylpiperazin- 1 - yl)phenyl]methyl} amino)methyl] [ 1 Jbenzofuro [3 ,2-d]pyrimidin-4(3 H)-one;
8-bromo-2-[(phenyloxy)methyl][l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
8-bromo-2-[(phenylamino)methyl][l]benzofuro[3,2-d]pyrimidin-4(3H)-one; 8-bromo-2- { [3-(dimethylamino)pyrrolidin- 1 -yljmethyl} [ 1 ]benzofuro[3 ,2-d]pyrimidin- 4(3H)-one;
8-bromo-2- { [(2-chlorophenyl)(methyl)amino]methyl} [ 1 ]benzofuro[3 ,2-d]pyrimidin- 4(3H)-one;
8-bromo-2- { [(2-fluorophenyl)amino]methyl} [ 1 ]benzofuro[3 ,2-d]pyrimidin-4(3H)-one; l-[(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)methyl]-N-[3- (dimethylamino)propyl]prolinamide;
8-bromo-2-[( { [3-(dimethylamino)phenyl]methyl} amino)methyl] [ 1 ]benzofuro[3 ,2- d]pyrimidin-4(3H)-one;
8-bromo-2- { [(4-fluorophenyl)amino]methyl} [ 1 ]benzofuro[3 ,2-d]pyrimidin-4(3H)-one;
8-cyclopropyl-2-[(4-methylpiperazin-l-yl)methyl][l]benzoflιro[3,2-d]pyrimidin-4(3H)- one;
8-bromo-2-(morpholin-4-ylmethyl)[l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
2-{4-[(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)methyl]piperazin- 1 -yl} -N,N-dimethylacetamide;
8-bromo-2- { [4-(N,N-diethylglycyl)piperazin- 1 -yljmethyl} [ 1 ]benzofuro[3 ,2- d]pyrimidin-4(3H)-one;
2- [(3 -aminopyrrolidin- 1 -yl)methyl] -8 -bromo [ 1 ]benzofuro [3 ,2-d]pyrimidin-4(3H)-one;
8-Acetyl-2-[(4-methylpiperazin- 1 -yl)methyl] [ 1 ]benzofuro[3 ,2- J]pyrimidin-4(3H)-one;
8-bromo-2-(2-chlorophenyl)[l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
8-chloro-2-(2-chlorophenyl)[l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
2-(2-chlorophenyl)-8-(methyloxy)[l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
8-bromo-2-(2-chloro-4-nitrophenyl)[l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
2-(4-amino-2-chlorophenyl)-8-bromo[l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
8-bromo-2-{2-chloro-4-[(piperidin-4-ylmethyl)amino]phenyl}[l]benzofuro[3,2- d]pyrimidin-4(3H)-one;
8-bromo-2-(2,6-dichlorophenyl)[l]benzofuro[3,2-d]pyrimidin-4(3H)-one; 8-bromo-2-(2,5-dichlorophenyl)[l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
8-bromo-2-(2-bromophenyl)[l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
8-bromo-2-(2-chloro-6-fluorophenyl)[l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
8-bromo-2-(2-iodophenyl)[l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
8-bromo-2-[2-chloro-4-(dimethylamino)phenyl][l]benzoflιro[3,2-d]pyrimidin-4(3H)- one;
8-bromo-2-(2-chloro-4-fluorophenyl)[l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
8-bromo-2-(3-bromopyridin-4-yl)[l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
8-bromo-2-(2-chloro-5-fluorophenyl)[l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
8-bromo-2-(4-methylpiperazin-l-yl)[l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
8-bromo-2-[ 1 -(4-methylpiperazin- 1 -yl)ethyl] [ 1 ]benzofuro[3 ,2-d]pyrimidin-4(3H)-one;
2-(2-chlorophenyl)-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidine-8-carbonitrile;
8-bromo-2-[2-(l H-imidazol- 1 -yl)phenyl] [ 1 ]benzofuro[3 ,2-d]pyrimidin-4(3H)-one;
2-(4-amino-2-methylphenyl)-8-bromo[l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
8-bromo-2-(2-hydroxyphenyl)[l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
8-bromo-2-(2,4-dichlorophenyl)[l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
methyl 4-(4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)benzoate;
8-bromo-2-{2-methyl-4-[(piperidin-4-ylmethyl)amino]phenyl}[l]benzofuro[3,2- d]pyrimidin-4(3H)-one;
8-bromo-2-(2,3-dichlorophenyl)[l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
2-(2-chlorophenyl)-8-phenyl[l]benzoflιro[3,2-d]pyrimidin-4(3H)-one;
8-bromo-2-pyridin-2-yl[l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
8-bromo-2-(2-fluorophenyl)[l]benzoflιro[3,2-d]pyrimidin-4(3H)-one;
8-bromo-2-(2-thienyl)[l]benzofuro[3,2-d]pyrimidin-4(3H)-one; 8-bromo-2-(2-methylphenyl)[l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
8-bromo-2-{2-methyl-4-[(tetrahydrofuran-3-ylmethyl)amino]phenyl}[l]benzofuro[3,2- d]pyrimidin-4(3H)-one;
N-[4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-3-methylphenyl]- N~2~,N~2 — dimethylglycinamide;
2-(2-chlorophenyl)-8-methyl[l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
8-bromo-2-[2-chloro-3-(methyloxy)phenyl][l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
8-bromo-2-[2-(trifluoromethyl)phenyl][l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
8-bromo-2-[2-bromo-4,5-bis(methyloxy)phenyl][l]benzofuro[3,2-d]pyrimidin-4(3H)- one;
8-bromo-2-[2-fluoro-5-(methyloxy)phenyl][l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
8-bromo-2-[2-chloro-3 ,4-bis(methyloxy)phenyl] [ 1 ]benzofuro[3 ,2-d]pyrimidin-4(3H)- one;
8-bromo-2-(5-chloro-2-thienyl)[l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
8-bromo-2-(2,6-difluorophenyl)[l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
2-(2-chlorophenyl)-7-hydroxy[l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
2-(2-chlorophenyl)-8-nitro[l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
2-(2-chlorophenyl)-8-hydroxy[l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
8-bromo-2-[( { [2-(4-methylpiperazin- 1 - yl)phenyl]methyl} amino)methyl] [ 1 ]benzofuro [3 ,2-d]pyrimidin-4(3 H)-one;
8-bromo-2-( { [4-(4-methylpiperazin- 1 -yl)phenyl] amino } methyl) [ 1 Jbenzofuro [3,2- d]pyrimidin-4(3H)-one;
8-bromo-2-({[3-(dimethylamino)phenyl]amino}methyl)[l]benzofuro[3,2-d]pyrimidin- 4(3H)-one;
8-bromo-2-(2-ethylphenyl)[l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
8-bromo-2-[2-bromo-5-(methyloxy)phenyl][l]benzoflιro[3,2-d]pyrimidin-4(3H)-one;
8-bromo-2-[2-chloro-4-(methyloxy)phenyl][l]benzoflιro[3,2-d]pyrimidin-4(3H)-one; N-[4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)phenyl]acetamide;
8-bromo-2-({4-[2-(dimethylamino)ethyl]piperazin-l-yl}methyl)[l]benzofuro[3,2- d]pyrimidin-4(3H)-one;
8-bromo-2- { [4-(2-morpholin-4-ylethyl)piperazin- 1 -yl]methyl} [ 1 ]benzoflιro[3 ,2- d]pyrimidin-4(3H)-one;
2-(2-chlorophenyl)-9-(methyloxy)[l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
8-bromo-2- { [4-( 1 -methylpiperidin-4-yl)piperazin- 1 -yljmethyl} [ 1 Jbenzofuro [3 ,2- d]pyrimidin-4(3H)-one;
8-bromo-2-(lH-imidazol-2-yl)[l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
8-bromo-2-(l,3-thiazol-2-yl)[l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
2-(2-chloro-6-fluorophenyl)-8-cyclopropyl[l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
8-bromo-2-[2-(methylthio)phenyl][l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
8-bromo-2-[2-(l-methylethyl)phenyl][l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
2-(2-chlorophenyl)-8-(trifluoromethyl)[l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
N-[2-(2-chlorophenyl)-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-8-yl]acetamide;
3-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-N- cyclohexylbenzamide;
8-bromo-2-(3-chlorophenyl)benzofuro[3,2-d]pyrimidin-4(3H)-one;
8-bromo-2-(4-chlorophenyl)benzofuro[3,2-d]pyrimidin-4(3H)-one;
8-bromo-2-(4-bromophenyl)benzofuro[3,2-d]pyrimidin-4(3H)-one;
2-(2-chlorophenyl)-N-methyl-4-oxo-3,4-dihydrobenzofuro[3,2-d]pyrimidine-8- carboxamide;
2-{[3-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)phenyl]oxy}-N- (cyclopropylmethyl)acetamide;
3-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-N- (phenylmethyl)benzamide;
8-bromo-2-[(2-methyl- lH-imidazol- 1 -yl)methyl] [ 1 ]benzofuro[3 ,2-d]pyrimidin-4(3H)- one; 3-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-N-(2- methylpropyl)benzamide;
8-bromo-2-[( { [4-(4-methylpiperazin- 1 -yl)phenyl]methyl} oxy)methyl] [ 1 ]benzofuro[3 ,2- d]pyrimidin-4(3H)-one;
l-[(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)methyl]pyrrolidine-3- carboxylic acid;
8-bromo-2-[( { [4-(dimethylamino)phenyl]methyl} amino)methyl] [ 1 ]benzofuro[3 ,2- d]pyrimidin-4(3H)-one;
8-bromo-2-(2-pyrrolidin-l-ylethyl)[l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
8-bromo-2-[(4-methylpiperazin- 1 -yl)carbonyl] [ 1 ]benzoflιro[3 ,2-d]pyrimidin-4(3H)-one;
8-bromo-2-(3,5-dichloropyridin-4-yl)[l]benzoflιro[3,2-d]pyrimidin-4(3H)-one;
2-{[(3S)-3-aminopyrrolidin-l-yl]methyl}-8-bromo[l]benzofuro[3,2-d]pyrimidin-4(3H)- one;
2- {[(3R)-3-aminopyrrolidin- 1 -yljmethyl} -8-bromo[ 1 ]benzofuro[3,2-d]pyrimidin-4(3H)- one;
8-bromo-2-( {3 - [(piperidin-4-ylmethyl)amino]pyrrolidin- 1 -yl} methyl) [ 1 Jbenzofuro [3,2- d]pyrimidin-4(3H)-one;
8-bromo-2-[2-( { [4-(4-methylpiperazin- 1 - yl)phenyl]methyl} amino)ethyl] [ 1 ]benzofuro[3 ,2-d]pyrimidin-4(3H)-one;
8-bromo-2-(3-chloropyridin-4-yl)[l]benzoflιro[3,2-d]pyrimidin-4(3H)-one;
8-bromo-2-({4-[2-(lH-imidazol-l-yl)ethyl]piperazin-l-yl}methyl)[l]benzofuro[3,2- d]pyrimidin-4(3H)-one;
8-bromo-2-[(4,4-difluoropiperidin- 1 -yl)methyl] [ 1 ]benzofuro[3 ,2-d]pyrimidin-4(3H)- one;
8-bromo-2-(4-methylpiperazin-l-yl)[l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
8-bromo-2-(4-bromo-2-chlorophenyl)[l]benzoflιro[3,2-d]pyrimidin-4(3H)-one;
8-bromo-2-[ 1 -(4-methylpiperazin- 1 -yl)ethyl] [ 1 ]benzofuro[3 ,2-d]pyrimidin-4(3H)-one;
8-bromo-2- { [3-(methylamino)pyrrolidin- 1 -yl]methyl} [ 1 ]benzofuro[3 ,2-d]pyrimidin- 4(3H)-one; 8-bromo-2-[(lS,4S)-2,5-diazabicyclo[2.2.1]hept-2-ylmethyl][l]benzoflιro[3,2- d]pyrimidin-4(3H)-one;
2-(2-chlorophenyl)-8-(methylamino)[l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
N-[4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-3- chlorophenyl] acetamide;
8-bromo-2-(piperidin-4-ylmethyl)[l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
8-bromo-2-(3-methyl-lH-indazol-5-yl)[l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
2-(2-chloro-4-nitrophenyl)-8-(methyloxy)[l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
2-(2-chlorophenyl)-8-[(trifluoromethyl)oxy][l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
8-bromo-2-(2-chloro-5-nitrophenyl)[l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
N-[4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-3-chlorophenyl]- N~2~,N~2 — dimethylglycinamide;
8-bromo-2-[2-chloro-4-(methylsulfonyl)phenyl][l]benzoflιro[3,2-d]pyrimidin-4(3H)- one;
2-(4-amino-2-chlorophenyl)-8-(methyloxy)[l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
8-bromo-2- {[(1,1 -dimethyl-2-morpholin-4-ylethyl)amino]methyl} [ 1 ]benzofuro [3 ,2- d]pyrimidin-4(3H)-one;
2-{[(lR,5S)-3-amino-8-azabicyclo[3.2.1]oct-8-yl]methyl}-8-bromo[l]benzofuro[3,2- d]pyrimidin-4(3H)-one;
8-bromo-2-[(8aS)-hexahydropyrrolo[l,2-a]pyrazin-2(lH)-ylmethyl][l]benzoflιro[3,2- d]pyrimidin-4(3H)-one;
8-bromo-2- { [(3 S)-3 -hydroxypyrrolidin- 1 -yl]methyl} [ 1 ]benzofuro [3 ,2-d]pyrimidin- 4(3H)-one;
2-(5-amino-2-chlorophenyl)-8-bromo[l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
N-[4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-3-chlorophenyl]- N~3~,N~3~- dimethyl-beta-alaninamide;
8-bromo-2-(2-chloro-4-{[4-(dimethylamino)butyl]amino}phenyl)[l]benzofuro[3,2- d]pyrimidin-4(3H)-one; l,l-dimethylethyl 4-[({3-chloro-4-[8-(methyloxy)-4-oxo-3,4-dihydro[l]benzofuro[3,2- d]pyrimidin-2-yl]phenyl} amino)methyl]piperidine- 1 -carboxylate;
2-{2-chloro-4-[(piperidin-4-ylmethyl)amino]phenyl}-8-(methyloxy)[l]benzofuro[3,2- d]pyrimidin-4(3H)-one;
8-bromo-2-{2-chloro-4-[(lH-imidazol-4-ylmethyl)amino]phenyl}[l]benzofuro[3,2- d]pyrimidin-4(3H)-one;
N-[4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-3- chlorophenyl]piperidine-3-carboxamide;
8-bromo-2-(piperazin- 1 -ylmethyl)[ 1 ]benzofuro[3,2-d]pyrimidin-4(3H)-one;
8-bromo-2-{[(lS,4S)-5-methyl-2,5-diazabicyclo[2.2.1]hept-2- yl]methyl}[l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
N-[4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-3- chlorophenyl]pyridine-4-carboxamide;
2-(2-chloro-4-fluorophenyl)-8-(methyloxy)[l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
2-(2-chloro-5-nitrophenyl)-8-(methyloxy)[l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
methyl 4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-3- chlorobenzoate;
8-bromo-2-morpholin-4-yl[l]benzoflιro[3,2-d]pyrimidin-4(3H)-one;
8-bromo-2-(4-chloropyridin-3-yl)[l]benzoflιro[3,2-d]pyrimidin-4(3H)-one;
8-bromo-2-[(2-ethyl- 1 H-imidazol- 1 -yl)methyl] [ 1 ]benzofuro[3 ,2-d]pyrimidin-4(3H)- one;
8-bromo-2-[(4-ethylpiperazin-l-yl)methyl][l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
8-bromo-2-({4-[2-(methyloxy)ethyl]piperazin-l-yl}methyl)[l]benzofuro[3,2- d]pyrimidin-4(3H)-one;
2-(3-chloropyridin-4-yl)-8-(methyloxy)[l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
8-bromo-2-(2 -methyl- 1 -pyrrolidin- 1 -ylpropyl)[ 1 ]benzofuro[3 ,2-d]pyrimidin-4(3H)-one;
8-bromo-2-{2-chloro-4-[(pyrrolidin-3-ylmethyl)amino]phenyl}[l]benzofuro[3,2- d]pyrimidin-4(3H)-one; 8-bromo-2-{2-chloro-4-[(2-piperidin-3-ylethyl)amino]phenyl}[l]benzoflιro[3,2- d]pyrimidin-4(3H)-one;
8-bromo-2- [(2-chlorophenyl)(4-methylpiperazin- 1 -yl)methyl] [ 1 ]benzofuro [3,2- d]pyrimidin-4(3H)-one;
8-bromo-2-piperidin-4-yl[l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
8-bromo-2-piperidin-3-yl[l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
8-bromo-2-{2-chloro-4-[(piperidin-3-ylmethyl)amino]phenyl}[l]benzofuro[3,2- d]pyrimidin-4(3H)-one;
N-[4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-3-chlorophenyl]- 4-(dimethylamino)butanamide;
N-[4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-3- chlorophenyl]piperidine-4-carboxamide;
N-[4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-3-chlorophenyl]- 3-(lH-imidazol-4-yl)propanamide;
8-bromo-2-(2-chloro-4- { [( 1 -methylpiperidin-4- yl)methyl] amino } phenyl) [ 1 Jbenzofuro [3 ,2-d]pyrimidin-4(3H)-one;
8-bromo-2- { [(3R)-3-hydroxypyrrolidin- 1 -yl]methyl} [ 1 ]benzofuro[3 ,2-d]pyrimidin- 4(3H)-one;
8-bromo-2-{[(pyridin-4-ylmethyl)amino]methyl}[l]benzofuro[3,2-d]pyrimidin-4(3H)- one;
8-bromo-2- { [(2-pyridin-4-ylethyl)amino]methyl} [ 1 ]benzofuro[3 ,2-d]pyrimidin-4(3H)- one;
2-(azetidin- 1 -ylmethyl)-8-bromo[ 1 ]benzofuro[3,2-d]pyrimidin-4(3H)-one;
2- { [ 1 -(2-aminoethyl)piperidin-4-yl]methyl} -8-bromo[ 1 ]benzofuro[3 ,2-d]pyrimidin- 4(3H)-one;
8-bromo-2-pyrrolidin-2-yl[l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
2-(3-methyl-lH-indazol-5-yl)-9-(methyloxy)[l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
2-(3-amino-lH-indazol-5-yl)-9-(methyloxy)[l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
8-bromo-2-[ 1 -(4-methylpiperazin- 1 -yl)propyl] [ 1 ]benzofuro[3 ,2-d]pyrimidin-4(3H)-one;
2-(2-amino-5-chloropyridin-4-yl)-8-bromo[l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
2-[amino(phenyl)methyl]-8-bromo[l]benzofuro[3,2-d]pyrimidin-4(3H)-one; 2-(l-aminoethyl)-8-bromo[l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
8-bromo-2-[(2-phenyl- lH-imidazol- 1 -yl)methyl] [ 1 ]benzofuro[3 ,2-d]pyrimidin-4(3H)- one;
N~2~- [(8-bromo-4-oxo-3,4-dihydro[l]benzoflιro[3,2-d]pyrimidin-2-yl)methyl]-2- methylalaninamide;
N-[4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-3- chlorophenyl]pyrrolidine-3-carboxamide;
8-bromo-2-(2-chloro-4-{[3-(dimethylamino)-2,2- dimethylpropyl] amino } phenyl) [ 1 ]benzofuro [3 ,2-d]pyrimidin-4(3H)-one;
8-bromo-2-(phenylamino)[l]benzoflιro[3,2-d]pyrimidin-4(3H)-one;
N-[4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-3-chlorophenyl]- 3 -piperidin- 1 -ylpropanamide;
N-[4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-3-chlorophenyl]- 2-(lH-imidazol-4-yl)acetamide;
8-bromo-2-[(3-hydroxyazetidin-l-yl)methyl][l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
8-bromo-2-(l-methylpiperidin-3-yl)[l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
8-bromo-2-(l-methylpiperidin-4-yl)[l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
8-bromo-2-pyrrolidin-3-yl[l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
2-[(4-acetylpiperazin- 1 -yl)methyl]-8-bromo[ 1 ]benzofuro[3,2-d]pyrimidin-4(3H)-one;
8-bromo-2-[(4-methylpiperazin- 1 -yl)(phenyl)methyl] [ 1 ]benzofuro[3 ,2-d]pyrimidin- 4(3H)-one;
N-[(8-bromo-4-oxo-3,4-dihydro[l]benzoflιro[3,2-d]pyrimidin-2-yl)methyl]-2- methylalanine;
8-bromo-2- {[(1,1 -dimethyl-2-pyrrolidin- 1 -ylethyl)amino]methyl} [ 1 ]benzofuro[3 ,2- d]pyrimidin-4(3H)-one;
8-bromo-2- {[(1,1 -dimethyl-2 -piperidin- 1 -ylethyl)amino]methyl} [ 1 ]benzofuro[3 ,2- d]pyrimidin-4(3H)-one;
8-bromo-2-{[(lS,4S)-5-(4-methylphenyl)-2,5-diazabicyclo[2.2.1]hept-2- yl]methyl}[l]benzofuro[3,2-d]pyrimidin-4(3H)-one; 8-bromo-2-{[3-(dimethylamino)propyl]amino}[l]benzoflιro[3,2-d]pyrimidin-4(3H)- one;
8-bromo-2-(piperidin-3-ylmethyl)[l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
2-(aminomethyl)-8-bromo[l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
8-bromo-2-pyrrolidin- 1 -yl[ 1 ]benzofuro[3 ,2-d]pyrimidin-4(3H)-one;
8-bromo-2-{2-chloro-4-[(2-piperidin-4-ylethyl)amino]phenyl}[l]benzoflιro[3,2- d]pyrimidin-4(3H)-one;
2-azetidin-3-yl-8-bromo[l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
8-bromo-2-[(cyclopentylamino)methyl][l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
8-bromo-2-({[2-(dimethylamino)ethyl]amino}methyl)[l]benzofuro[3,2-d]pyrimidin- 4(3H)-one;
8-bromo-2-[(4-methylpiperidin- 1 -yl)methyl] [ 1 ]benzoflιro[3 ,2-d]pyrimidin-4(3H)-one;
2-(l-amino-l-methylethyl)-8-chloro[l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
2-(l ,4'-bipiperidin- 1 '-ylmethyl)-8-bromo[ 1 ]benzofuro[3,2-d]pyrimidin-4(3H)-one;
2-(l-acetylpiperidin-4-yl)-8-bromo[l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
2-(3-amino-5-chloro-lH-indazol-6-yl)-8-bromo[l]benzoflιro[3,2-d]pyrimidin-4(3H)- one;
8-bromo-2-[ 1 -(N,N-dimethyl-beta-alanyl)piperidin-4-yl] [ 1 ]benzofuro[3 ,2-d]pyrimidin- 4(3H)-one;
8-bromo-2- { 1 -[4-(dimethylamino)butanoyl]piperidin-4-yl} [ 1 ]benzofuro[3 ,2- d]pyrimidin-4(3H)-one;
2-[l-(lH-benzimidazol-5-ylcarbonyl)piperidin-4-yl]-8-bromo[l]benzofuro[3,2- d]pyrimidin-4(3H)-one;
8-bromo-2- { 1 -[3-(methyloxy)propanoyl]piperidin-4-yl} [ 1 ]benzoflιro[3,2-d]pyrimidin- 4(3H)-one;
8-bromo-2-[ 1 -(N,N-dimethylglycyl)piperidin-4-yl] [ 1 ]benzofuro[3 ,2-d]pyrimidin-4(3H)- one;
2-[4-(aminomethyl)-2-chloro-5-fluorophenyl]-8-bromo[l]benzofuro[3,2-d]pyrimidin- 4(3H)-one; 8-bromo-2-( {[1,1 -dimethyl-2-(4-methylpiperazin- 1 - yl)ethyl]amino}methyl)[l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
8-bromo-2-(piperidin-4-ylamino)[l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
8-bromo-2-(pyridin-3-ylamino)[l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
8-bromo-2-[ 1 -(4-methylpiperazin- 1 -yl)-2-phenylethyl] [ 1 ]benzoflιro[3 ,2-d]pyrimidin- 4(3H)-one;
8-bromo-2- { [(2R,6S)-2,6-dimethylpiperazin- 1 -yljmethyl} [ 1 ]benzofuro[3 ,2-d]pyrimidin- 4(3H)-one;
N-[4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-3-chlorophenyl]- 2-piperidin-4-ylacetamide;
8-bromo-2-{[(lS,4S)-5-{[4-(trifluoromethyl)phenyl]methyl}-2,5- diazabicyclo[2.2.1]hept-2-yl]methyl}[l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
8-bromo-2-{3-[(2-methylpropyl)oxy]pyridin-4-yl}[l]benzofuro[3,2-d]pyrimidin-4(3H)- one;
2-(3-amino-lH-indazol-5-yl)-8-bromo[l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
8-bromo-2-(l-methylazetidin-3-yl)[l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
1,1-dimethylethyl [4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2- yl)cyclohexyl] carbamate;
2-(4-aminocyclohexyl)-8-bromo[l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
l-[(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)methyl]-N-[(2- chlorophenyl)methyl]pyrrolidine-3-carboxamide;
8-bromo-2-( { [4-(4-methylpiperazin- 1 -yl)phenyl]methyl} amino) [ 1 ]benzofuro [3,2- d]pyrimidin-4(3H)-one;
8-bromo-2-[(l-methylpiperidin-3-yl)methyl][l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
8-bromo-2- {4-[2-(dimethylamino)ethyl]piperazin- 1 -yl} [ 1 ]benzofuro[3,2-d]pyrimidin- 4(3H)-one;
8-bromo-2-[4-( 1 -methylpiperidin-4-yl)piperazin- 1 -yl] [ 1 ]benzofuro [3 ,2-d]pyrimidin- 4(3H)-one;
8-bromo-2-[(3aR,6aS)-hexahydropyrrolo[3,4-c]pyrrol-2(lH)- ylmethyl] [ 1 ]benzofuro [3 ,2-d]pyrimidin-4(3H)-one; 8-iodo-2-[(4-methylpiperazin-l-yl)methyl][l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
2-[(2-aminoethyl)amino]-8-bromo[l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
8-bromo-2- { [( 1 -methylpropyl)amino]methyl} [ 1 ]benzofuro[3 ,2-d]pyrimidin-4(3H)-one;
8-bromo-2-[l-(tetrahydrofuran-3-ylcarbonyl)piperidin-4-yl][l]benzofuro[3,2- d]pyrimidin-4(3H)-one;
8-bromo-2-[ 1 -(phenylcarbonyl)piperidin-4-yl] [ 1 ]benzoflιro[3 ,2-d]pyrimidin-4(3H)-one;
8-bromo-2-( { [(2-chlorophenyl)methyl]amino } methyl) [ 1 Jbenzofuro [3 ,2-d]pyrimidin- 4(3H)-one;
8-bromo-2-[ 1 -(pyridin-4-ylcarbonyl)piperidin-4-yl] [ 1 ]benzofuro[3 ,2-d]pyrimidin- 4(3H)-one;
8-bromo-2-[ 1 -(phenylacetyl)piperidin-4-yl] [ 1 ]benzofuro[3 ,2-d]pyrimidin-4(3H)-one;
8-bromo-2-[(4-phenylpiperidin- 1 -yl)methyl] [ 1 ]benzofuro[3 ,2-d]pyrimidin-4(3H)-one;
8-bromo-2- { [(phenylmethyl)amino]methyl} [ 1 ]benzofuro[3 ,2-d]pyrimidin-4(3H)-one;
8-bromo-2-[(4-pyridin-3-ylpiperazin-l-yl)methyl][l]benzofuro[3,2-d]pyrimidin-4(3H)- one;
8-bromo-2-[(2,3-dihydro- 1 H-inden- 1 -ylamino)methyl] [ 1 ]benzofuro[3 ,2-d]pyrimidin- 4(3H)-one;
8-bromo-2-[(4-hydroxypiperidin- 1 -yl)methyl] [ 1 ]benzofuro[3 ,2-d]pyrimidin-4(3H)-one;
2-(2-amino-5-chloropyrimidin-4-yl)-8-bromo[l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
8-bromo-2-[( 1 -methylpiperidin-4-yl)methyl] [ 1 ]benzofuro[3 ,2-d]pyrimidin-4(3H)-one;
8-bromo-2-[4-(N,N-diethylglycyl)piperazin- 1 -yl] [ 1 ]benzofuro[3 ,2-d]pyrimidin-4(3H)- one;
8-bromo-2- {4-[3-(dimethylamino)propyl]piperazin- 1 -yl} [ 1 Jbenzofuro [3, 2-d]pyrimidin- 4(3H)-one;
8-bromo-2- { [4-(2-hydroxyethyl)piperazin- 1 -yljmethyl} [ 1 Jbenzofuro [3 ,2-d]pyrimidin- 4(3H)-one;
N-[4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-3-chlorophenyl]- 3 -morpholin-4-ylpropanamide;
N-[4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-3- chlorophenyljbenzamide; N-[4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-3- chlorophenyljcyclohexanecarboxamide;
N-[4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-3- chlorophenyljcyclopentanecarboxamide;
8-bromo-2-[(cyclobutylamino)methyl][l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
8-bromo-2- { [4-(phenylmethyl)piperidin- 1 -yljmethyl} [ 1 ]benzofuro[3 ,2-d]pyrimidin- 4(3H)-one;
8-bromo-2-{[(pyridin-2-ylmethyl)amino]methyl}[l]benzofuro[3,2-d]pyrimidin-4(3H)- one;
8-bromo-2-[( { [3-(methyloxy)phenyl]methyl} amino)methyl] [ 1 ]benzofuro[3 ,2- d]pyrimidin-4(3H)-one;
8-bromo-2-( { [(3 -chlorophenyl)methyl]amino } methyl) [ 1 ]benzofuro [3 ,2-d]pyrimidin- 4(3H)-one;
8-bromo-2-[(4-morpholin-4-ylpiperidin- 1 -yl)methyl] [ 1 ]benzofuro[3 ,2-d]pyrimidin- 4(3H)-one;
8-bromo-2- { [(furan-2-ylmethyl)amino]methyl} [ 1 ]benzofuro[3 ,2-d]pyrimidin-4(3H)- one;
8-bromo-2-({[2-(lH-imidazol-4-yl)ethyl]amino}methyl)[l]benzofuro[3,2-d]pyrimidin- 4(3H)-one;
8-bromo-2-[(4-phenylpiperazin- 1 -yl)methyl] [ 1 ]benzoflιro[3 ,2-d]pyrimidin-4(3H)-one;
8-bromo-2-[( { [4-(methyloxy)phenyl]methyl} amino)methyl] [ 1 ]benzofuro[3 ,2- d]pyrimidin-4(3H)-one;
8-bromo-2-{[(2,3-dihydroxypropyl)amino]methyl}[l]benzofuro[3,2-d]pyrimidin-4(3H)- one;
8-bromo-2-[(2,3-dihydro-lH-inden-2-ylamino)methyl][l]benzofuro[3,2-d]pyrimidin- 4(3H)-one;
l-[(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)methyl]-N-[(3- chlorophenyl)methyl]pyrrolidine-3-carboxamide;
8-bromo-2-[5-chloro-2-(methylamino)pyridin-4-yl][l]benzofuro[3,2-d]pyrimidin- 4(3H)-one;
l-[(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)methyl]-N- (phenylmethyl)pyrrolidine-3-carboxamide; 8-bromo-2- { [3 -(morpholin-4-ylcarbonyl)pyrrolidin- 1 -yl]methyl} [ 1 ]benzofuro [3,2- d]pyrimidin-4(3H)-one;
8-bromo-2-[( { [2-(methyloxy)phenyl]methyl} amino)methyl] [ 1 ]benzoflιro[3 ,2- d]pyrimidin-4(3H)-one; ethyl l-[(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2- yl)methyl]pyrrolidine-3-carboxylate; l-[(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)methyl]pyrrolidine-3- carboxamide; l-[(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)methyl]-N-[(4- chlorophenyl)methyl]pyrrolidine-3-carboxamide;
8-bromo-2-( {3-[(4-hydroxypiperidin- 1 -yl)carbonyl]pyrrolidin- 1 - yl}methyl)[l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
8-bromo-2-( 1 -methylpyrrolidin-2-yl)[ 1 ]benzofuro[3 ,2-d]pyrimidin-4(3H)-one;
N-[4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-3-chlorophenyl]- 2-chloro-6-methylpyridine-4-carboxamide;
N-[4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-3-chlorophenyl]- 3-chloropyridine-4-carboxamide;
N-[4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-3-chlorophenyl]- 2-chloropyridine-4-carboxamide;
8-bromo-2-( { [2-(methyloxy)ethyl] amino } methyl) [ 1 ]benzofuro [3 ,2-d]pyrimidin-4(3H)- one;
8-bromo-2- { [4-(3 -chlorophenyl)piperazin- 1 -yl]methyl} [ 1 ]benzofuro [3 ,2-d]pyrimidin- 4(3H)-one;
N-[4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-3-chlorophenyl]- 4-chlorobenzamide;
N-[4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-3-chlorophenyl]- 3-chlorobenzamide;
N-[4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-3-chlorophenyl]- 3-(methyloxy)benzamide;
8-bromo-2- {(3R)-5-oxo- 1 -[(1R)- 1 -phenylethyl]pyrrolidin-3-yl} [ 1 ]benzofuro[3,2- d]pyrimidin-4(3H)-one;
8-bromo-2-(5 -chloro-2- { [2-(dimethylamino)ethyl] amino } pyridin-4-yl) [ 1 Jbenzofuro [3 ,2- d]pyrimidin-4(3H)-one; 2-amino-N-[4-(8-bromo-4-oxo-3,4-dihydro[l]benzoflιro[3,2-d]pyrimidin-2-yl)-3- chlorophenyl]pyridine-4-carboxamide;
N-[4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-3-chlorophenyl]- 4-(methyloxy)benzamide;
8-bromo-2-[(4-hydroxy-2-oxopyrrolidin- 1 -yl)methyl] [ 1 ]benzofuro[3 ,2-d]pyrimidin- 4(3H)-one;
8-bromo-2- { [3 -(hydroxymethyl)pyrrolidin- 1 -yljmethyl} [ 1 Jbenzofuro [3 ,2-d]pyrimidin- 4(3H)-one;
8-bromo-2-{[(pyrrolidin-3-ylmethyl)oxy]methyl}[l]benzoflιro[3,2-d]pyrimidin-4(3H)- one;
8-bromo-2-(l-methylpyrrolidin-3-yl)[l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
8-bromo-2-[l-(pyridin-4-ylmethyl)pyrrolidin-3-yl][l]benzofuro[3,2-d]pyrimidin-4(3H)- one;
N-[4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-3-chlorophenyl]- 6-methylpyridine-3-carboxamide;
N-[4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-3- chlorophenyl]pyridine-3-carboxamide;
N-[4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-3- chlorophenyl]pyridine-2-carboxamide;
N-[4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-3-chlorophenyl]- 2,6-dichloropyridine-4-carboxamide;
8-bromo-2-{[(lS,4S)-5-ethyl-2,5-diazabicyclo[2.2.1]hept-2- yl]methyl}[l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
8-bromo-2- { [(3R,5 S)-3 ,5-dimethylpiperazin- 1 -yl]methyl} [ 1 ]benzofuro[3 ,2-d]pyrimidin- 4(3H)-one;
1,1-dimethylethyl 2-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2- yl)morpholine-4-carboxylate;
8-bromo-2-morpholin-2-yl[l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
8-bromo-2-[3-(piperidin-4-yloxy)isoxazol-5-yl][l]benzofuro[3,2-d]pyrimidin-4(3H)- one;
8-bromo-2-(l,2,3,4-tetrahydroisoquinolin-6-yl)[l]benzoflιro[3,2-d]pyrimidin-4(3H)- one; 8-bromo-2-(5,6,7,8-tetrahydroimidazo[l,2-a]pyrazin-2-yl)[l]benzofuro[3,2- d]pyrimidin-4(3H)-one;
2- [3 -(aminomethyl)phenyl] -8-bromo [ 1 ]benzofuro [3 ,2-d]pyrimidin-4(3H)-one;
8-bromo-2-(4-piperazin-l-ylphenyl)[l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
8-bromo-2-(5-oxo-l-piperidin-4-ylpyrrolidin-3-yl)[l]benzofuro[3,2-d]pyrimidin-4(3H)- one;
8-bromo-2-[(3S)-piperidin-3-ylmethyl][l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
8-bromo-2- { [3 -(hydroxymethyl)piperidin- 1 -yljmethyl} [ 1 Jbenzofuro [3 ,2-d]pyrimidin- 4(3H)-one;
8-bromo-2- { [(3R)-3-fluoropyrrolidin- 1 -yl]methyl} [ 1 ]benzoflιro[3 ,2-d]pyrimidin-4(3H)- one;
8-bromo-2- { [(3 S)-3-fluoropyrrolidin- 1 -yl]methyl} [ 1 ]benzofuro[3 ,2-d]pyrimidin-4(3H)- one;
8-bromo-2-[(2-hydroxyethyl)amino][l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)acetic acid;
8-bromo-2-(3,9-diazaspiro[5.5]undec-3-ylmethyl)[l]benzofuro[3,2-d]pyrimidin-4(3H)- one;
N-[4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-3-chlorophenyl]- 5 -methylpyrazine-2-carboxamide;
N-[4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-3- chlorophenyl]tetrahydrofuran-3-carboxamide;
N-[3-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-4- chlorophenyl]pyridine-4-carboxamide;
8-bromo-2-[(3R)-piperidin-3-ylmethyl][l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
8-bromo-2- { [(3R,5 S)-3 ,4,5-trimethylpiperazin- 1 -yl]methyl} [ 1 ]benzofuro[3 ,2- d]pyrimidin-4(3H)-one;
8-bromo-2-[(3-fluoropiperidin-l-yl)methyl][l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
8-bromo-2-[(3 ,3-difluoropyrrolidin- 1 -yl)methyl] [ 1 ]benzofuro[3 ,2-d]pyrimidin-4(3H)- one; N-[4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-3-chlorophenyl]- 2-(methyloxy)acetamide;
methyl [4-(8-bromo-4-oxo-3,4-dihydro[l]benzomro[3,2-d]pyrimidin-2-yl)-3- chlorophenyl] carbamate;
N-[4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-3- chlorophenyl]furan-2-carboxamide;
N-[4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-3-chlorophenyl]- 2-pyridin-3 -ylacetamide;
N-[4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-3- chlorophenyl]pyrazine-2-carboxamide;
N-[4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-3- chlorophenyl]isoxazole-5-carboxamide;
l-[(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)methyl]-N-(pyridin- 4-ylmethyl)pyrrolidine-3-carboxamide;
N-[3-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-4-chlorophenyl]- 2-chloropyridine-4-carboxamide;
N-[3-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-4- chlorophenyl]pyridine-3-carboxamide;
N-[4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-3- chlorophenyl]pyrimidine-5-carboxamide;
N-[3-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-4- chlorophenyl]pyrimidine-5-carboxamide;
8-bromo-2-{[(lS,4S)-5-methyl-2,5-diazabicyclo[2.2.2]oct-2- yl]methyl}[l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
8-bromo-2-((2,5-dihydro-lH-pyrrol-l-yl)methyl)benzoflιro[3,2-d]pyrimidin-4(3H)-one;
8-bromo-2-((7-hydroxy-2-azabicyclo[2.2.1]heptan-2-yl)methyl)benzofuro[3,2- d]pyrimidin-4(3H)-one;
8-bromo-2-(((3S,4S)-3,4-dihydroxypyrrolidin-l-yl)methyl)benzofuro[3,2-d]pyrimidin- 4(3H)-one;
methyl 6-({[4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-3- chlorophenyl] amino } carbonyl)pyridine-3 -carboxylate;
N-[4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-3-chlorophenyl]- 2-(methyloxy)pyridine-4-carboxamide; 5-bromo-N-[4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-3- chlorophenyl]pyridine-2-carboxamide;
N-[3-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-4-chlorophenyl]- 2-(methyloxy)pyridine-4-carboxamide;
5-bromo-N-[3-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-4- chlorophenyl]pyridine-2-carboxamide;
N-[4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-3-chlorophenyl]- 5-chloropyridine-2-carboxamide;
N-[3-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-4-chlorophenyl]- 5-chloropyridine-2-carboxamide;
N-[4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-3- chlorophenyl] furan-3 -carboxamide;
N-[3-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-4- chlorophenyl] furan-3 -carboxamide;
N-[4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-3-chlorophenyl]- 6-(methyloxy)pyridine-3-carboxamide;
8-bromo-2- { [(2R)-2-(hydroxymethyl)pyrrolidin- 1 -yl]methyl} [ 1 ]benzofuro[3 ,2- d]pyrimidin-4(3H)-one;
N-[4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-3-chlorophenyl]- 5-(hydroxymethyl)pyridine-2-carboxamide;
8-bromo-2- [(3 -hydro xy-3 -methylpyrrolidin- 1 -yl)methyl] [ 1 Jbenzofuro [3 ,2-d]pyrimidin- 4(3H)-one;
6-({[4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-3- chlorophenyl] amino } carbonyl)pyridine-3 -carboxylic acid;
8-bromo-2- { [(3R,4S)-3 ,4-dihydroxypyrrolidin- 1 -yl]methyl} [ 1 ]benzofuro[3 ,2- d]pyrimidin-4(3H)-one;
2-[4-(aminomethyl)phenyl]-8-bromo[l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
8-bromo-2-[(4-oxo-3-phenyl-l,3,8-triazaspiro[4.5]dec-l-yl)methyl][l]benzofuro[3,2- d]pyrimidin-4(3H)-one;
8-bromo-2-[l-methyl-3-(piperidin-4-yloxy)-lH-pyrazol-5-yl][l]benzofuro[3,2- d]pyrimidin-4(3H)-one;
2-[2-(aminomethyl)-l,3-thiazol-4-yl]-8-bromo[l]benzofuro[3,2-d]pyrimidin-4(3H)-one; 8-bromo-2-{3-[(dimethylamino)methyl]phenyl}[l]benzofuro[3,2-d]pyrimidin-4(3H)- one;
2-(4-aminophenyl)-8-bromo[l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
2-(3-aminophenyl)-8-bromo[l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
4-(acetylamino)-N-[3-(8-bromo-4-oxo-3,4-dihydro[l]benzoflιro[3,2-d]pyrimidin-2-yl)- 4-chlorophenyl]benzamide;
N-[3-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-4-chlorophenyl]- 6-(methyloxy)pyridine-3-carboxamide;
8-bromo-2-{4-[(dimethylamino)methyl]phenyl}[l]benzofuro[3,2-d]pyrimidin-4(3H)- one;
8-bromo-2-{4-[(morpholin-2-ylmethyl)oxy]phenyl}[l]benzofuro[3,2-d]pyrimidin- 4(3H)-one;
N-[4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)phenyl]pyridine-4- carboxamide;
N-[3-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-4- chlorophenyl]tetrahydrofuran-3-carboxamide;
N-[4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-3-chlorophenyl]- 5 -methylisoxazole-3 -carboxamide;
N-[4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-3- chlorophenyl]isoxazole-3-carboxamide;
N-{(3R)-l-[(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2- yl)methyl]pyrrolidin-3 -yl} acetamide;
N-{(3S)-l-[(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2- yl)methyl]pyrrolidin-3 -yl} acetamide;
8-bromo-2- { [(2S)-2-(hydroxymethyl)pyrrolidin- 1 -yl]methyl} [ 1 ]benzofuro[3 ,2- d]pyrimidin-4(3H)-one;
8-fluoro-2-[(4-methylpiperazin- 1 -yl)methyl] [ 1 ]benzofuro[3 ,2-d]pyrimidin-4(3H)-one;
l-[(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)methyl]-3- hydroxypyrrolidine-3 -carboxylic acid;
8-bromo-2- { [(3 S)-3 -(methyloxy)pyrrolidin- 1 -yljmethyl} [ 1 Jbenzofuro [3 ,2-d]pyrimidin- 4(3H)-one; l-[(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)methyl]azetidine-3- carboxylic acid;
N-[3-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-4- chlorophenyljbenzamide;
N-[3-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-4- chlorophenyl]isoxazole-5-carboxamide;
N-[4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-3-chlorophenyl]- 1 ,3-oxazole-2-carboxamide;
N-[4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-3-chlorophenyl]- 1 ,3-oxazole-5-carboxamide;
8-bromo-2-(l,2,3,4-tetrahydroisoquinolin-5-yl)[l]benzoflιro[3,2-d]pyrimidin-4(3H)- one;
2-[trans-4-(aminomethyl)cyclohexyl]-8-bromo[l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
8-bromo-2-(2-piperidin-4-ylpyrazolo[l,5-a]pyrimidin-6-yl)[l]benzofuro[3,2- d]pyrimidin-4(3H)-one;
8-bromo-2-(((lR,4R)-5-methyl-2,5-diazabicyclo[2.2.1]heptan-2- yl)methyl)benzofuro[3,2-d]pyrimidin-4(3H)-one;
2-[4-(aminomethyl)-2-chlorophenyl]-8-bromo[l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
2-(3-aminocyclohexyl)-8-bromo[l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
8-bromo-2-{2-chloro-4-[(dimethylamino)methyl]phenyl}[l]benzofuro[3,2-d]pyrimidin- 4(3H)-one;
8-bromo-2-{4-[(methylamino)methyl]phenyl}[l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-3-chloro-N-(2- piperidin- 1 -ylethyl)benzamide;
8-bromo-2-[phenyl(piperazin-l-yl)methyl][l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
8-bromo-2-(2-chloro-4-{[(2-piperidin-l- ylethyl)amino]methyl}phenyl)[l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
1 , 1 -dimethylethyl 4-( { [4-(8-bromo-4-oxo-3 ,4-dihydro [ 1 ]benzoflιro [3 ,2-d]pyrimidin-2- yl)-3-chlorophenyl]methyl}amino)piperidine-l-carboxylate;
8-bromo-2-{2-chloro-4-[(piperidin-4-ylamino)methyl]phenyl}[l]benzoflιro[3,2- d]pyrimidin-4(3H)-one; N-KSR^^-l-fCS-bromo^-oxo-S^-dihydroCηbenzofuroCS^-^pyrimidin-l-yOmethyl]- 4-hydroxypyrrolidin-3 -yl} piperidine-4-carboxamide;
N-[4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-3-chlorophenyl]- 1 -methyl- 1 H-imidazole-4-carboxamide;
N-[4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-3-chlorophenyl]- 1 -methyl- 1 H-imidazole-2-carboxamide;
N-[4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-3-chlorophenyl]- 1 -methyl- lH-pyrazole-3-carboxamide;
N-[3-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-4- chlorophenyl]piperidine-4-carboxamide;
3-amino-N-[4-(8-bromo-4-oxo-3,4-dihydro[l]benzoflιro[3,2-d]pyrimidin-2-yl)-3- chlorophenyl]-lH-indazole-5-carboxamide;
N~5~-(2-aminoethyl)-N~2~-[4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2- d]pyrimidin-2-yl)-3-chlorophenyl]pyridine-2,5-dicarboxamide;
N-[4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-3-chlorophenyl]- lH-pyrrolo[2,3-b]pyridine-5-carboxamide;
N-[4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-3-chlorophenyl]- 6-(lH-pyrazol-l-yl)pyridine-3-carboxamide;
N-[4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-3- chlorophenyl]quinoxaline-2-carboxamide;
8-bromo-2-[(3-hydroxy-8-azabicyclo[3.2.1 ]oct-8-yl)methyl] [ 1 ]benzofuro[3,2- d]pyrimidin-4(3H)-one;
8-bromo-2-[(3-hydroxypiperidin-l-yl)methyl][l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
8-bromo-2- { [(2-hydroxyethyl)(methyl)amino]methyl} [ 1 ]benzofuro [3 ,2-d]pyrimidin- 4(3H)-one;
8-bromo-2- { [(3R)-3-hydroxypiperidin- 1 -yljmethyl} [ 1 ]benzofuro[3 ,2-d]pyrimidin- 4(3H)-one;
2-{[bis(2-hydroxypropyl)amino]methyl}-8-bromo[l]benzofuro[3,2-d]pyrimidin-4(3H)- one;
8-bromo-2- { [(3 S)-3 -hydroxypiperidin- 1 -yl]methyl} [ 1 ]benzofuro [3 ,2-d]pyrimidin- 4(3H)-one; N-[4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-3-chlorophenyl]- l,8-naphthyridine-2-carboxamide;
N-[4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-3-chlorophenyl]- l,2,3-thiadiazole-4-carboxamide;
8-bromo-2-[(3-ethyl-3-hydroxypyrrolidin-l-yl)methyl][l]benzofuro[3,2-d]pyrimidin- 4(3H)-one;
8-bromo-2- { [(3R)-3-(methyloxy)pyrrolidin- 1 -yljmethyl} [ 1 ]benzofuro[3 ,2-d]pyrimidin- 4(3H)-one;
8-bromo-2-{[(2,3-dihydroxypropyl)(methyl)amino]methyl}[l]benzoflιro[3,2- d]pyrimidin-4(3H)-one;
2-{[bis(2-hydroxyethyl)amino]methyl}-8-bromo[l]benzofuro[3,2-d]pyrimidin-4(3H)- one; l-[(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)methyl]-N-[(2- chlorophenyl)methyl] -3 -hydroxypyrrolidine-3 -carboxamide;
8-bromo-2-[(7-hydroxy-2-azabicyclo[2.2.1]hept-2-yl)methyl][l]benzofuro[3,2- d]pyrimidin-4(3H)-one;
8-bromo-2-{[(7S)-7-hydroxy-2-azabicyclo[2.2.1]hept-2-yl]methyl}[l]benzofuro[3,2- d]pyrimidin-4(3H)-one; l-[4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-3-chlorophenyl]- 3 -pyridin-4-ylurea;
8-bromo-2-[(4-methylpiperazin-l-yl)methyl]pyrido[l,2-e]purin-4(3H)-one;
8-bromo-2-{[(3S)-3-hydroxypyrrolidin-l-yl]methyl}pyrido[l,2-e]purin-4(3H)-one;
8-bromo-2- { [(3 S)-3-hydroxypyrrolidin- 1 -yljmethyl} -7-methyl[ 1 ]benzofuro[3 ,2- d]pyrimidin-4(3H)-one;
8-bromo-2-[(2-bromophenyl)methyl][l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
8-bromo-2-{2-chloro-4-[(methylamino)methyl]phenyl}[l]benzofuro[3,2-d]pyrimidin- 4(3H)-one;
2-(lH-benzimidazol-6-yl)-8-bromo[l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
hydroxy(2- { [(3 S)-3-hydroxypyrrolidin- 1 -yljmethyl} -8-methyl-4-oxo-3 ,4- dihydro[l]benzofuro[3,2-d]pyrimidin-9-yl)oxoammonium; 8-bromo-2-{[(lS,6R)-9-methyl-3,9-diazabicyclo[4.2.1]non-3- yl]methyl}[l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
8-bromo-2-[(4-methyl- 1 ,4-diazepan- 1 -yl)methyl] [ 1 ]benzofuro[3 ,2-d]pyrimidin-4(3H)- one;
8-bromo-2-{2-chloro-4-[(cyclohexylamino)methyl]phenyl}[l]benzofuro[3,2- d]pyrimidin-4(3H)-one;
6-bromo-2- { [(3 S)-3-hydroxypyrrolidin- 1 -yljmethyl} -9-(methyloxy)[ 1 ]benzofuro[3 ,2- d]pyrimidin-4(3H)-one;
8-chloro-2- { [(3 S)-3 -hydroxypyrrolidin- 1 -yl]methyl} [ 1 ]benzofuro [3 ,2-d]pyrimidin- 4(3H)-one;
8-bromo-2-(2-chloro-4-{[(l-methylethyl)amino]methyl}phenyl)[l]benzofuro[3,2- d]pyrimidin-4(3H)-one;
8-bromo-2- { [(2-piperidin- 1 -ylethyl)amino]methyl} [ 1 ]benzofuro[3 ,2-d]pyrimidin-4(3H)- one;
9-{[(3S)-3-hydroxypyrrolidin-l-yl]methyl}-lH-pyrimido[4',5':4,5]flιro[2,3-g]indazol- 7(8H)-one;
hydroxy(2- { [(3 S)-3 -hydroxypyrrolidin- 1 -yljmethyl} -8-methyl-4-oxo-3 ,4- dihydro[l]benzofuro[3,2-d]pyrimidin-7-yl)oxoammonium;
8-bromo-2- { [(3 S)-3 -hydroxypyrrolidin- 1 -yljmethyl} -6-methyl[ 1 ]benzofuro[3 ,2- d]pyrimidin-4(3H)-one;
8-bromo-2-(2-chloro-4- { [( 1 -methylpiperidin-4- yl)amino]methyl}phenyl)[l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
8-bromo-2- { [(3 S)-3 -hydroxypyrrolidin- 1 -yl]methyl} -7-(methyloxy)[ 1 ]benzofuro[3 ,2- d]pyrimidin-4(3H)-one;
8-bromo-7-hydroxy-2- { [(3 S)-3 -hydroxypyrrolidin- 1 -yl]methyl} [ 1 ]benzofuro[3 ,2- d]pyrimidin-4(3H)-one;
8-bromo-7-hydroxy-2- [(4-methylpiperazin- 1 -yl)methyl] [ 1 Jbenzofuro [3 ,2-d]pyrimidin- 4(3H)-one;
6-amino-N-[4-(8-bromo-4-oxo-3,4-dihydro[l]benzofuro[3,2-d]pyrimidin-2-yl)-3- chlorophenyl]pyridine-3-carboxamide;
9-amino-2- { [(3 S)-3 -hydroxypyrrolidin- 1 -yljmethyl} -8-methyl[ 1 ]benzofuro[3 ,2- d]pyrimidin-4(3H)-one; 8-bromo-2-{3-[(piperidin-4-ylmethyl)amino]-lH-indazol-6-yl}[l]benzofuro[3,2- d]pyrimidin-4(3H)-one;
2- { [(3 S)-3-hydroxypyrrolidin- 1 -yl]methyl} -8-(methylthio)[ 1 ]benzofuro[3 ,2- d]pyrimidin-4(3H)-one;
2- { [(3 S)-3 -hydroxypyrrolidin- 1 -yljmethyl} -8- [(phenylmethyl)amino] [ 1 ]benzofuro [3,2- d]pyrimidin-4(3H)-one;
8- { [2-(3 -chlorophenyl)ethyl]amino } -2- { [(3 S)-3 -hydroxypyrrolidin- 1 - yljmethyl} [l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
8-({2-[2,3-bis(methyloxy)phenyl]ethyl}amino)-2-{[(3S)-3-hydroxypyrrolidin-l- yl]methyl}[l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
8-(butylamino)-2- { [(3 S)-3 -hydroxypyrrolidin- 1 -yljmethyl} [ 1 ]benzofuro[3 ,2- d]pyrimidin-4(3H)-one;
8-bromo-2- { [(3 S)-3 -hydroxypyrrolidin- 1 -yljmethyl} -9-(methyloxy)[ 1 ]benzofuro[3 ,2- d]pyrimidin-4(3H)-one;
2- { [(3 S)-3 -hydroxypyrrolidin- 1 -yl]methyl} -9-(methyloxy) [ 1 ]benzofuro [3 ,2- d]pyrimidin-4(3H)-one;
8-(cyclohexylamino)-2- { [(3 S)-3 -hydroxypyrrolidin- 1 -yl]methyl} [ 1 ]benzofuro [3,2- d]pyrimidin-4(3H)-one;
8-(3 -hydroxyprop- 1 -yn- 1 -yl)-2- [(4-methylpiperazin- 1 -yl)methyl] [ 1 Jbenzofuro [3 ,2- d]pyrimidin-4(3H)-one;
2- { [(3 S)-3 -hydroxypyrrolidin- 1 -yljmethyl} -8-(methyloxy) [ 1 ]benzofuro [3 ,2- d]pyrimidin-4(3H)-one;
( 13bR)- 11 -bromo- 1,2,3,13b-tetrahydro-7H-[ 1 ]benzofuro[3 ,2- d]pyrrolo[r,2':3,4]imidazo[l,5-a]pyrimidin-7-one;
(13bS)-l l-bromo-l,2,3,13b-tetrahydro-7H-[l]benzofuro[3,2- d]pyrrolo[r,2':3,4]imidazo[l,5-a]pyrimidin-7-one;
8-(3 -hydroxyprop- 1 -yn- 1 -yl)-2- { [(3 S)-3 -hydroxypyrrolidin- 1 - yl]methyl}[l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
8-(3 -hydroxypropyl)-2- { [(3 S)-3 -hydroxypyrrolidin- 1 -yl]methyl} [ 1 ]benzofuro [3 ,2- d]pyrimidin-4(3H)-one;
8-(6-hydroxyhex- 1 -yn- 1 -yl)-2- { [(3 S)-3 -hydroxypyrrolidin- 1 - yl]methyl}[l]benzofuro[3,2-d]pyrimidin-4(3H)-one; 8-(6-hydroxyhexyl)-2- { [(3 S)-3 -hydroxypyrrolidin- 1 -yl]methyl} [ 1 ]benzofuro [3,2- d]pyrimidin-4(3H)-one;
8-ethynyl-2- { [(3 S)-3 -hydroxypyrrolidin- 1 -yl]methyl} [ 1 ]benzofuro [3 ,2-d]pyrimidin- 4(3H)-one;
2- { [(3 S)-3 -hydroxypyrrolidin- 1 -yljmethyl} -8- [(trimethylsilyl)ethynyl] [ 1 Jbenzofuro [3 ,2- d]pyrimidin-4(3H)-one;
8-ethyl-2- { [(3 S)-3 -hydroxypyrrolidin- 1 -yl]methyl} [ 1 ]benzofuro[3 ,2-d]pyrimidin-4(3H)- one;
2- { [(3 S)-3-hydroxypyrrolidin- 1 -yl]methyl} -8- { [(2- methylphenyl)methyl] amino } [ 1 ]benzofuro [3 ,2-d]pyrimidin-4(3H)-one;
8- { [(2-fluorophenyl)methyl] amino } -2- { [(3 S)-3 -hydroxypyrrolidin- 1 - yl]methyl}[l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
2- { [(3 S)-3 -hydroxypyrrolidin- 1 -yljmethyl} -8-[(pyridin-2- ylmethyl)amino] [ 1 ]benzofuro [3 ,2-d]pyrimidin-4(3H)-one;
2- { [(3 S)-3 -hydroxypyrrolidin- 1 -yl]methyl} -8-( { [3 - (trifluoromethyl)phenyl]methyl}amino)[l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
8-{[(2,4-difluorophenyl)methyl]amino}-2-{[(3S)-3-hydroxypyrrolidin-l- yl]methyl}[l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
2- { [(3 S)-3-hydroxypyrrolidin- 1 -yl]methyl} -8-( { [2- (methyloxy)phenyl]methyl}amino)[l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
2- { [(3 S)-3 -hydroxypyrrolidin- 1 -yl]methyl} -8-( { [3 - (methyloxy)phenyl]methyl}amino)[l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
2- { [(3 S)-3-hydroxypyrrolidin- 1 -yl]methyl} -8-( { [4- (methyloxy)phenyl]methyl}amino)[l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
2- { [(3 S)-3 -hydroxypyrrolidin- 1 -yl]methyl} -9-[(2-methylpropyl)oxy] [ 1 ]benzofuro[3 ,2- d]pyrimidin-4(3H)-one;
8-bromo-2-[(2S)-pyrrolidin-2-yl][l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
8-bromo-2-[(2R)-pyrrolidin-2-yl][l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
8- { [2-(3 -fluorophenyl)ethyl] amino } -2- { [(3 S)-3 -hydroxypyrrolidin- 1 - yljmethyl} [l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
2-{[(3S)-3-hydroxypyrrolidin-l-yl]methyl}-8-({2-[3- (trifluoromethyl)phenyl]ethyl}amino)[l]benzofuro[3,2-d]pyrimidin-4(3H)-one;
Figure imgf000405_0001
Figure imgf000406_0001
Figure imgf000407_0001
16. A pharmaceutical composition comprising the compound according to claim 1 , or a pharmaceutically acceptable salt thereof, and a pharmaceutically acceptable carrier, excipient, or diluent.
17. A method of inhibiting PIM, CDC7 or CK2 in a cell, comprising contacting the cell, in which inhibition of PIM, CDC7 or CK2 is desired, with the compound according to claim 1 , or a pharmaceutically acceptable salt thereof.
18. A method of treating a disease or condition comprising administering to a patient, in need of said treatment, the compound according to claim 1, or a pharmaceutically acceptable salt thereof, wherein the disease or condition is a cancer selected from pancreatic cancer, prostate cancer, hepatocellular carcinoma, lymphomas, leukemias, colorectal.
19. A method of treating a disease or condition comprising administering to a patient, in need of said treatment, a pharmaceutical composition comprising the compound according to claim 1 , or a pharmaceutically acceptable salt thereof, and a pharmaceutically acceptable carrier, excipient, or diluent, wherein the disease or condition is a cancer selected from pancreatic cancer, prostate cancer, hepatocellular carcinoma, lymphomas, leukemias, colorectal.
20. The method according to claim 18, further comprising administering radiation treatment or one or more therapeutic angents selected from Camptothecin, Topotecan, 9-Nitrocamptothecin, 9-Aminocamptothecin, Karenitecin, Irinotecan, Etoposide, Etoposide Phosphate, Teniposide, Amsacrine, Razoxane, Dexrazoxane, Mechlorethamine, Cyclophosphamide, Ifosfamide, Chlorambucil, Melphalan, Thiotepa, Trenimon, Triethylenemelamine, Dianhydrogalactitol, Dibromodulcitol, Busulfan, dimethylsulfate, Chloroethylnitrosourea, BCNU, CCNU, Methyl-CCNU, Streptozotocin, Chlorozotocin, Prednimustine, Estramustine, Procarbazine, Dacarbazine, Hexamethylmelamine, Pentamethylmelamine, Temozolomide, Cisplatin, Carboplatin, Oxaliplatin, Bleomycin, Dactinomycin, Mithramycin, Rapamycin, Mitomycin C, Daunorubicin, Doxorubicin, Epirubicin, Idarubicin, Methotrexate, Edatrexate, Trimethoprim, Nolatrexed, Raltitrexed, Hydroxyurea, 5- fluorouracil, ixabepilone, Ftorafur, Capecitabine, Furtulon, Eniluracil, ara-C, 5- azacytidine, Gemcitabine, Mercaptopurine, Thioguanine, Pentostatin, antisense DNA, antisense RNA, an antisense DNA/RNA hybrid, a ribozyme, ultraviolet radiation, Vincristine, Vinblastine, Paclitaxel, Docetaxel, L- Asparaginase, a kinase inhibitor, Imatinib, Mitotane, Aminoglutethimide, Diethylstilbestrol, Ethinyl estradiol, Tamoxifen, Anastrozole, Testosterone propionate, Fluoxymesterone, Flutamide, Leuprolide, Prednisone, Hydroxyprogesterone caproate, Medroxyprogesterone acetate, Megestrol acetate, Interferon-alfa, and Interleukin.
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