WO2009081358A1 - Sonde à fibre optique - Google Patents

Sonde à fibre optique Download PDF

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Publication number
WO2009081358A1
WO2009081358A1 PCT/IB2008/055442 IB2008055442W WO2009081358A1 WO 2009081358 A1 WO2009081358 A1 WO 2009081358A1 IB 2008055442 W IB2008055442 W IB 2008055442W WO 2009081358 A1 WO2009081358 A1 WO 2009081358A1
Authority
WO
WIPO (PCT)
Prior art keywords
fibre
illumination
target
optic probe
light
Prior art date
Application number
PCT/IB2008/055442
Other languages
English (en)
Inventor
Jagadeesh Chandra Bose Rantham Prabhakara
Sarif Kumar Naik
Satish Prasad Rath
Original Assignee
Koninklijke Philips Electronics N.V.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Koninklijke Philips Electronics N.V. filed Critical Koninklijke Philips Electronics N.V.
Priority to CN2008801219413A priority Critical patent/CN101903762B/zh
Publication of WO2009081358A1 publication Critical patent/WO2009081358A1/fr

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B6/00—Light guides; Structural details of arrangements comprising light guides and other optical elements, e.g. couplings
    • G02B6/24—Coupling light guides
    • G02B6/241—Light guide terminations
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N2021/6484—Optical fibres

Definitions

  • the invention relates to a fibre-optic probe, particularly for use in spectral analysis of biological tissue.
  • the United States patent US5822072 discusses a fused fibre-optic probe useful for conducting spectral measurements.
  • the fused fibre-optic probe comprises a probe tip having a specific geometrical configuration, an exciting optical fibre and at least one collection optical fibre fused within a housing preferably made of silica.
  • the specific geometrical configurations in which the probe tip can be shaped include a slanted probe tip with an angle greater than 0°, an inverted cone-shaped probe tip, and a lens head.
  • the fused fibre-optic probe is used to transmit excitation optical energy to a sample medium, and also to transmit optical signal, received from the sample medium, to a signal analyzer.
  • the fibre-optic probe comprises an illumination fibre having a length and a tip proximal to a target, wherein the illumination fibre is configured to conduct light from a light source to the target.
  • the fibre-optic probe also includes at least one collection fibre having a slanted end proximal to the target, wherein the collection fibre is configured to conduct light away from the target.
  • the illumination fibre is further configured to taper along at least part of its length towards the tip.
  • the tapered tip Due to the tapered tip, a more focused or narrower beam of light is emitted from the illumination fibre, thus reducing the overlap between the excitation and collection light.
  • the slanted end of the collection fibre helps in increasing the area of collection. The combination of these two features, i.e., the tapered tip and the slanted end, leads to a greater increase in the intensity of the output spectrum compared to using either of these features individually, thereby increasing the accuracy of determination of spectral peak positions.
  • FIGURE 1 illustrates a fibre-optic probe having a tapered illumination fibre and two collection fibres having slanted ends;
  • FIGURE 2a illustrates a head-on view of a single collection fibre
  • FIGURE 2b illustrates a transverse cross-sectional view of the fibre-optic probe shown in FIGURE 1 ;
  • FIGURES 3a and 3b illustrate an embodiment of the disclosed fibre-optic probe having an adjustable illumination fibre
  • FIGURE 4 illustrates an optical spectroscopy system including the fibre-optic probe shown in FIGURE 1.
  • optical spectroscopy deals with the interaction of electromagnetic radiation with matter, mainly at wavelengths in the ultraviolet (10 nm to 100 nm), visible light (400 to 700 nm), near-infrared (1000 nm to 1 ⁇ m) and infrared (10 ⁇ m to 1 mm) ranges.
  • Screening patients for oral cancer based on the results of optical spectroscopy measurements involves irradiating a target sample (e.g., biological tissue within the mouth of a subject) with electromagnetic radiation, measuring the amount of light absorbed, emitted and/or scattered from the sample, and analyzing and interpreting the spectral components of the measured light.
  • a promising optical technique is laser induced fluorescence spectroscopy
  • LIFS also called auto-fluorescence spectroscopy
  • AFS auto-fluorescence spectroscopy
  • a tissue of interest is excited by a light source, which can be a laser or a high intensity broadband light source and the fluorescence spectrum of the tissue is measured. Additional information about LIFS may be found in the article "Fluorescence Spectroscopy of Neoplastic and Non- Neoplastic Tissue” by Nirmala Ramanujam, Neoplasia, Vol. 2, Nos. 1-2, January- April 2000, pp. 89-117. Though most of the description in this document may refer to LIFS by way of illustration, it is to be noted that the disclosed fibre-optic probe may be used with other spectroscopic techniques as well.
  • a fibre-optic probe is useful in irradiating and collecting data from hard-to- reach parts of a human anatomy.
  • the design of the probe is critical, as it can affect the light delivery to and light propagation into the tissue, the collection efficiency (i.e., the total number of photons collected vs. the total number of photons launched) and the origin of the collected light.
  • the configuration of the illumination (i.e., excitation) and collection fibres in the fibre-optic device plays an important role in deciding the quality of the recorded spectra.
  • the design parameters include choice of single fibre vs. multiple fibres, the size of the illumination and collection fibres, the aperture of the light/excitation source, and the source-to-detector separation.
  • fibre-optic probes for biomedical optical spectroscopy
  • Urs Utzinger and Rebecca R. Richards-Kortum Journal of Biomedical Optics 8(1), 121- 147 (January 2003)
  • Depth-sensitive reflectance measurements using obliquely oriented fiber probes by Adrien Ming Jer Wang, Janelle Elise Bender, Joshua Pfefer, Urs Utzinger and Rebekah Anna Drezek, Journal of Biomedical Optics 10(4), 044017 (July/August 2005).
  • FIGURE 1 shows an embodiment of the disclosed fibre-optic probe 100.
  • An illumination fibre (IF) 102 transmits incident light 108 from a light source (402 shown in FIGURE 4) to a tissue of interest (TIS) 114.
  • Light that is returned 110, 112 after interaction with the tissue of interest 114 is collected by collection fibres (CF) 104, 106, to be transmitted to a processing unit (404 shown in FIGURE 4).
  • CF collection fibres
  • the illumination fibre 102 is tapered at the tip in order to produce a narrower beam of incident light. This reduces the interference of the incident light 108 with the light collected 110, 112 by the collection fibres 104, 106.
  • the collection fibres 104, 106 have slanted ends to maximize the collection area, i.e., the area of the tissue 114 from which reflected light is collected. Instead of reflected light, the collection fibres could also collect scattered light or emitted light, for instance, due to fluorescence from the tissue.
  • the ends of the collection fibres 104, 106 are shown to be slanted in a direction so as to make an acute angle with the longitudinal axis of the illumination fibre 102, an end slanting in the other direction, i.e., so as to make an obtuse angle with the longitudinal axis of the illumination fibre 102 is also considered. Furthermore, various angles for the slanted end are also considered, for example, 30°, 45°, etc. Though only two collection fibres 104, 106 are shown in the figure, a single collection fibre could be used as well. Alternatively, more than two collection fibres could also be used.
  • FIGURE 2a shows a single collection fibre 204 with a slanted end 202, when the fibre is oriented with the collection surface parallel to the plane of the page.
  • FIGURE 2b shows an end-view or a projection of a bundle of optical fibres in a fibre-optic probe 200 (i.e., when viewed from the tip end of the probe) consisting of a single illumination fibre 208 and six collection fibres 206a, 206b, 206c, 206d, 206e and 206f, each having the slanted end shown in FIGURE 2a.
  • the illumination fibre 102 Due to the slanted ends of the collection fibres 206a-206f, their projections on the plane of the page appear elliptical in cross-section.
  • the two circles 208a, 208b show the projections of the two end-cross sections of the illumination fibre208.
  • the illumination fibre 102 has a broad portion and a tapered portion culminating in a narrow tip.
  • the circle 208a illustrates the projection of the broader portion, while the smaller circle 208b is indicative of the tip.
  • the illumination fibre 102 shown in FIGURE 1 is pencil-shaped, other tapered shapes are also possible. For example, it might be possible to have an illumination fibre that is tapered all the way from one end, say the end connected to a light source, to the tip. Alternatively, other shapes such as multiple tapered portions interspersed with straight portions are also considered.
  • a fibre-optic probe 300 is provided with an adjustable illumination fibre 302.
  • a typical fibre-optic probe has overlapping illumination and collection areas to minimize the effect of tissue turbidity and the sampling area is usually in the range of 1 mm in diameter, such that fluorescence can be measured with sufficient signal-to-noise ratio.
  • the probe-to-target distance is known to significantly affect the intensity and the region (depth) of origin of fluorescence emission.
  • a fixed illumination area might not cover the entire tissue of interest in many cases. Under such circumstances, an adjustable excitation probe could help in covering desired areas of the tissue more accurately.
  • FIGURES 3a and 3b overcomes the abovementioned problems, in that it changes the illuminated region by varying the distance between the excitation fibre 302 and the target tissue 312 while retaining the increased collection efficiency of the disclosed fibre-optic probe designs.
  • the illumination fibre 302 may be moved up or down within the fibre-optic probe 300 along a longitudinal axis of the fibre-optic probe, in order to move the tip of the fibre farther away or close to the target tissue 312.
  • the longitudinal axis of the fibre-optic probe is also the longitudinal axis of the illumination fibre 302, and hence the movement of the illumination fibre 302 may be described as being along its own longitudinal axis as well.
  • the line arrows show the direction of the light that is incident on the tissue 312 as well as light that is collected by the collection fibres 306.
  • the block arrows illustrate the movement of the excitation or illumination fibre 302.
  • FIGURE 3a shows the illumination fibre 302 moved away from the tissue 312, in order to increase coverage by the excitation light
  • FIGURE 3b shows the illumination fibre 302 moved closer to the target tissue 312 for a more focused excitation.
  • the intensity of the illumination light source (402 in FIGURE 4) may need to be adjusted. Details of the adjustments required are available in the prior art documents cited previously.
  • the movement of the illumination fibre 302 may be effected through manual means, for example a handle or a screw mechanism attached to the illumination fibre 302. Alternatively, the movement may be effected automatically using stepper motors, and such.
  • FIGURE 4 A typical fluorescence spectroscopy system is shown in FIGURE 4. It consists of a light source (LS) 402, an illumination and collection system 100, and a detection and processing unit (DPU) 404 that can measure the emitted light as a function of wavelength.
  • LS light source
  • DPU detection and processing unit
  • the light source 402 may be a monochromatic source, e.g., a laser, or a source of high-intensity, broadband radiation.
  • the illumination and collection system 100 is the fibre-optic probe as embodied in the various embodiments in this disclosure. As mentioned previously, it serves to transmit the incident light from the light source 402 to the tissue TIS, as well as to conduct reflected, scattered or emitted light from the tissue TIS to the detection and processing unit 404.
  • the detection and processing unit 404 receives the light from the fibre-optic probe and performs a spectral analysis to detect the various frequency components in the received light. An example of such processing might be a simple Fourier transform operation that detects the spectral peaks in the received light. Other techniques, such as power spectral analysis, etc., are also considered.

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  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Optics & Photonics (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)

Abstract

Sonde (100) à fibre optique minimisant le recouvrement entre une lumière d'excitation et une lumière collectée dans un système de spectroscopie optique. La sonde à fibre optique comprend une fibre (102) d'éclairage présentant une longueur et un bout proximal par rapport à une cible, la fibre d'éclairage étant configurée de façon à conduire la lumière d'une source lumineuse à la cible. La sonde à fibre optique comprend également au moins une fibre (104, 106) de collecte présentant une extrémité biseautée proximale par rapport à la cible, la fibre de collecte étant configurée de façon à éloigner la lumière de la cible. La fibre d'éclairage est en outre configurée pour s'amincir sur au moins une partie de sa longueur en direction du bout et la face d'extrémité biseautée de la ou des fibres de collecte fait face à la face d'extrémité amincie en forme de crayon de la fibre d'éclairage.
PCT/IB2008/055442 2007-12-21 2008-12-19 Sonde à fibre optique WO2009081358A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN2008801219413A CN101903762B (zh) 2007-12-21 2008-12-19 光纤探针

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IN3082/CHE/2007 2007-12-21
IN3082CH2007 2007-12-21

Publications (1)

Publication Number Publication Date
WO2009081358A1 true WO2009081358A1 (fr) 2009-07-02

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PCT/IB2008/055442 WO2009081358A1 (fr) 2007-12-21 2008-12-19 Sonde à fibre optique

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RU (1) RU2010130472A (fr)
WO (1) WO2009081358A1 (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2885628A4 (fr) * 2012-08-16 2016-07-20 Univ Singapore Instrument de diagnostic et procédés se rapportant à la spectroscopie raman
CN103837235B (zh) * 2012-11-21 2016-05-11 福州高意通讯有限公司 一种拉曼光谱仪探测头和拉曼光谱仪系统

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5822072A (en) * 1994-09-30 1998-10-13 Lockheed Martin Energy Systems, Inc. Fiberoptic probe and system for spectral measurements
US5911017A (en) * 1996-07-31 1999-06-08 Visionex, Inc. Fiber optic interface for laser spectroscopic Raman probes
US20010012429A1 (en) * 1995-11-20 2001-08-09 Cirrex Corp. Method and apparatus for improved fiber optic light management

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7239782B1 (en) * 2004-09-03 2007-07-03 Chemimage Corporation Chemical imaging fiberscope

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5822072A (en) * 1994-09-30 1998-10-13 Lockheed Martin Energy Systems, Inc. Fiberoptic probe and system for spectral measurements
US20010012429A1 (en) * 1995-11-20 2001-08-09 Cirrex Corp. Method and apparatus for improved fiber optic light management
US5911017A (en) * 1996-07-31 1999-06-08 Visionex, Inc. Fiber optic interface for laser spectroscopic Raman probes

Also Published As

Publication number Publication date
CN101903762A (zh) 2010-12-01
RU2010130472A (ru) 2012-01-27
CN101903762B (zh) 2013-08-21

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