WO2009073820A3 - Conjugaison biopolymère-acide nucléique - Google Patents

Conjugaison biopolymère-acide nucléique Download PDF

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Publication number
WO2009073820A3
WO2009073820A3 PCT/US2008/085594 US2008085594W WO2009073820A3 WO 2009073820 A3 WO2009073820 A3 WO 2009073820A3 US 2008085594 W US2008085594 W US 2008085594W WO 2009073820 A3 WO2009073820 A3 WO 2009073820A3
Authority
WO
WIPO (PCT)
Prior art keywords
biopolymer
nucleic acid
acid conjugation
conjugation
oligonucleotide
Prior art date
Application number
PCT/US2008/085594
Other languages
English (en)
Other versions
WO2009073820A2 (fr
Inventor
Paul Hatala
Ryan Boomer
Markus Kurz
Original Assignee
Archemix Corp.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Archemix Corp. filed Critical Archemix Corp.
Publication of WO2009073820A2 publication Critical patent/WO2009073820A2/fr
Publication of WO2009073820A3 publication Critical patent/WO2009073820A3/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/16—Aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/35—Nature of the modification
    • C12N2310/351—Conjugate
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2330/00—Production
    • C12N2330/30—Production chemically synthesised

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicinal Preparation (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

Méthode de conjugaison d'un biopolymère, tel qu'un polyalkylène glycol (PAG) et d'un oligonucléotide consistant à combiner une biopolymère activé et un oligonucléotide dans une solution aqueuse à pH de 8.5 ou plus et à ajouter un DMSO afin d'éviter des réactions latérales et une dégradation du PEG.
PCT/US2008/085594 2007-12-04 2008-12-04 Conjugaison biopolymère-acide nucléique WO2009073820A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US538207P 2007-12-04 2007-12-04
US61/005,382 2007-12-04

Publications (2)

Publication Number Publication Date
WO2009073820A2 WO2009073820A2 (fr) 2009-06-11
WO2009073820A3 true WO2009073820A3 (fr) 2009-09-24

Family

ID=40718503

Family Applications (2)

Application Number Title Priority Date Filing Date
PCT/US2008/085596 WO2009073821A2 (fr) 2007-12-04 2008-12-04 Conjugaison biopolymère-acide nucléique
PCT/US2008/085594 WO2009073820A2 (fr) 2007-12-04 2008-12-04 Conjugaison biopolymère-acide nucléique

Family Applications Before (1)

Application Number Title Priority Date Filing Date
PCT/US2008/085596 WO2009073821A2 (fr) 2007-12-04 2008-12-04 Conjugaison biopolymère-acide nucléique

Country Status (1)

Country Link
WO (2) WO2009073821A2 (fr)

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040105839A1 (en) * 2001-11-28 2004-06-03 Myung-Ok Park Biologically active non-antigenic copolymer and conjugates thereof and methods for producing the same

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE469164T1 (de) * 2001-10-26 2010-06-15 Noxxon Pharma Ag Modifizierte l-nukleinsäure
LT3385384T (lt) * 2004-02-12 2020-09-25 Archemix Llc Aptamero terapija, naudinga su komplementu susijusių ligų gydymui

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040105839A1 (en) * 2001-11-28 2004-06-03 Myung-Ok Park Biologically active non-antigenic copolymer and conjugates thereof and methods for producing the same

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
SARAN ET AL.: "A Versatile Photocleavable Bifunctional Linker for Facile Synthesis of Substrate- DNA Conjugates for the Selection of Nucleic Acid Catalysts", BIOCONJUGAT. CHEM., 2007, Retrieved from the Internet <URL:http://mmi.missouri.edu/burke/Saran_PhotocleavableBioconjChem2007.pdf> [retrieved on 20090722] *

Also Published As

Publication number Publication date
WO2009073820A2 (fr) 2009-06-11
WO2009073821A2 (fr) 2009-06-11
WO2009073821A3 (fr) 2009-08-27

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