WO2009071251A2 - Modèle de peau - Google Patents

Modèle de peau Download PDF

Info

Publication number
WO2009071251A2
WO2009071251A2 PCT/EP2008/010146 EP2008010146W WO2009071251A2 WO 2009071251 A2 WO2009071251 A2 WO 2009071251A2 EP 2008010146 W EP2008010146 W EP 2008010146W WO 2009071251 A2 WO2009071251 A2 WO 2009071251A2
Authority
WO
WIPO (PCT)
Prior art keywords
membrane
agar
hydrogel
gelatin
skin
Prior art date
Application number
PCT/EP2008/010146
Other languages
German (de)
English (en)
Other versions
WO2009071251A3 (fr
Inventor
Daniela Prinz
Gerd Kutz
Christiane Bruns
Original Assignee
Cognis Ip Management Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cognis Ip Management Gmbh filed Critical Cognis Ip Management Gmbh
Publication of WO2009071251A2 publication Critical patent/WO2009071251A2/fr
Publication of WO2009071251A3 publication Critical patent/WO2009071251A3/fr

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
    • A61L27/60—Materials for use in artificial skin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/14—Macromolecular materials
    • A61L27/22—Polypeptides or derivatives thereof, e.g. degradation products
    • A61L27/24—Collagen
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
    • A61L27/52—Hydrogels or hydrocolloids

Definitions

  • the invention is in the field of devices for testing cosmetic raw materials and final formulations.
  • the object of the present invention was therefore to provide a skin substitute which reproducibly simulates human skin and in which various methods for testing raw materials on the skin can be simulated.
  • the present invention is an apparatus for testing cosmetic and pharmaceutical raw materials comprising a membrane which is stretched over a container containing a hydrogel as a supporting substrate for the membrane.
  • a membrane which is stretched over the hydrogel as a supporting substrate, are preferably collagen membranes as they are used in the food industry. These are preferably collagen membranes which can be used as sausage pelts.
  • membranes made by Naturin which are marketed under the names EWD-Casing, COFFI-foil or Classic Casing.
  • the surface tests carried out on the membranes are to have different surface conditions, such as e.g. Lipophilicity, surface roughness, surface tension, these can be achieved by selecting the membrane, by the choice and concentration of the gelling agent, by the use of humectants in various concentrations or by applying sebum lipids to the membrane surface.
  • hydrogel gels can be used, which were prepared with a variety of gelling agents.
  • it is hydrogels based on agar-agar, gelatin, hydroxyethyl cellulose or mixtures thereof.
  • Particularly preferred are the hydrogels based on agar-agar, since they can be conditioned at 32 ° C, since they do not melt.
  • Suitable containers for the skin substitute are all inert containers, preferably glass containers.
  • Petri dishes are used in particular.
  • humectants can be added to the hydrogels. These prevent premature drying of the measuring device. Furthermore, the surface properties of the membrane can be deliberately influenced by the humectants. As a preferred humectant glycerol is considered. With respect to the hydrogels, the humectants are used in amounts of from 0.1 to 25% by weight.
  • the skin model cosmetic and pharmaceutical raw materials can be tested before the dermatological or toxicological safety has been established.
  • the water vapor diffusion on the surface of the skin model can be determined with the aid of a Tewameter TM210 from Courage & Khazaka, Cologne before and after treatment with a raw material or a composition, but also corneometric (determination of the hydration of the surface of the skin model, eg with the aid of a corneometer CM820 from Courage & Khazaka, Cologne) and frictiometric measurements (eg with a Fritiometer® FR 700) are possible.
  • the spreading behavior of oils on the skin model accordinging to Zeidler, Fette Seifen Anstrichstoff, Vol. 87, no.
  • TEWL transdermal water loss
  • corneometry especially the skin models based on an agar gelatin or agar-agar hydrogel with EWD-Casing were suitable.
  • the swollen gelatin was added, and stirred using a magnetic stirrer until the gelatin was completely dissolved.
  • Phenoxyethanol was added and the gelatin mass cooled. Once the mass had reached a temperature of about 40 to 50 ° C, it was filled in petri dishes, which were filled to the brim.
  • Gelatinization of the gelatin takes about 30 minutes to 120 minutes, depending on the temperature and relative humidity in the environment.
  • the conditioning was achieved in a climate chamber at 20 ° C and 80 to 90% relative humidity over 15-2Oh.

Abstract

L'invention concerne un dispositif permettant de tester des matières premières cosmétiques et pharmaceutiques, qui comprend une membrane, tendue sur un récipient qui contient un hydrogel comme fond pour la membrane.
PCT/EP2008/010146 2007-12-08 2008-11-29 Modèle de peau WO2009071251A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102007059199.5 2007-12-08
DE200710059199 DE102007059199A1 (de) 2007-12-08 2007-12-08 Hautmodell

Publications (2)

Publication Number Publication Date
WO2009071251A2 true WO2009071251A2 (fr) 2009-06-11
WO2009071251A3 WO2009071251A3 (fr) 2009-08-13

Family

ID=40621266

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2008/010146 WO2009071251A2 (fr) 2007-12-08 2008-11-29 Modèle de peau

Country Status (2)

Country Link
DE (1) DE102007059199A1 (fr)
WO (1) WO2009071251A2 (fr)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4603105A (en) * 1982-05-27 1986-07-29 Kaplan Donald A Efficient screening methods for low probability variants
US4801548A (en) * 1984-10-09 1989-01-31 Kobayashi Pharmaceutical Co., Ltd. Petri dish for cultivating bacteria and method of inspecting drug susceptibility
US5834005A (en) * 1992-02-24 1998-11-10 Encelle, Inc. Bioartificial devices and cellular matrices therefor

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2006230315A (ja) * 2005-02-25 2006-09-07 Nitto Denko Corp 微生物検出用デバイス及び微生物の検出方法
JP2006230304A (ja) * 2005-02-25 2006-09-07 Nitto Denko Corp 微生物検出用デバイス及び微生物の検出方法

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4603105A (en) * 1982-05-27 1986-07-29 Kaplan Donald A Efficient screening methods for low probability variants
US4801548A (en) * 1984-10-09 1989-01-31 Kobayashi Pharmaceutical Co., Ltd. Petri dish for cultivating bacteria and method of inspecting drug susceptibility
US5834005A (en) * 1992-02-24 1998-11-10 Encelle, Inc. Bioartificial devices and cellular matrices therefor

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
CHEN CHUN-HUNG ET AL: "Novel living cell sheet harvest system composed of thermoreversible methylcellulose hydrogels" BIOMACROMOLECULES, Bd. 7, Nr. 3, März 2006 (2006-03), Seiten 736-743, XP002530849 ISSN: 1525-7797 *
DATABASE WPI Week 200662 Thomson Scientific, London, GB; AN 2006-598712 XP002530851 & JP 2006 230304 A (NIPPON SEIYAKU KK) 7. September 2006 (2006-09-07) *
DATABASE WPI Week 200663 Thomson Scientific, London, GB; AN 2006-606748 XP002530850 & JP 2006 230315 A (NIPPON SEIYAKU KK) 7. September 2006 (2006-09-07) *

Also Published As

Publication number Publication date
WO2009071251A3 (fr) 2009-08-13
DE102007059199A1 (de) 2009-06-10

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