WO2009071251A2 - Skin model - Google Patents

Skin model Download PDF

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Publication number
WO2009071251A2
WO2009071251A2 PCT/EP2008/010146 EP2008010146W WO2009071251A2 WO 2009071251 A2 WO2009071251 A2 WO 2009071251A2 EP 2008010146 W EP2008010146 W EP 2008010146W WO 2009071251 A2 WO2009071251 A2 WO 2009071251A2
Authority
WO
Grant status
Application
Patent type
Prior art keywords
membrane
characterized
device according
used
hydrogel
Prior art date
Application number
PCT/EP2008/010146
Other languages
German (de)
French (fr)
Other versions
WO2009071251A3 (en )
Inventor
Daniela Prinz
Gerd Kutz
Christiane Bruns
Original Assignee
Cognis Ip Management Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date

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Classifications

    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61LMETHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION, OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS, OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS, OR SURGICAL ARTICLES
    • A61L27/00Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/50Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
    • A61L27/60Materials for use in artificial skin
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61LMETHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION, OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS, OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS, OR SURGICAL ARTICLES
    • A61L27/00Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/14Macromolecular materials
    • A61L27/22Polypeptides or derivatives thereof, e.g. degradation products
    • A61L27/24Collagen
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61LMETHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION, OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS, OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS, OR SURGICAL ARTICLES
    • A61L27/00Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/50Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
    • A61L27/52Hydrogels or hydrocolloids

Abstract

The invention relates to a device for testing cosmetic and pharmaceutical raw materials, comprising a membrane that is streched over a container that comprises a hydrogel as supporting base for the membrane.

Description

skin model

Field of the Invention

The invention is in the field of devices for testing of cosmetic raw materials and final formulations.

State of the art

Before cosmetic raw materials or end formulations are introduced into the market, they are subjected to sensory tests as part of the development process. The raw materials or formulations are tested and toxicological release in healthy volunteers. Rate trained volunteers, the sensory characteristics using a profile that must be created for each substance to be tested. An example is here pointed out the methodology for determining the spreadability. The area, which spreads a cosmetic oil on the skin is placed in correlation with the perceived by consumers feeling of smoothness. However, such in vivo, tests have the disadvantage that only can be tested as toxicological harmless evaluated substances already. Furthermore, some measurements are machines not technically feasible in vivo. Artificial test surfaces, partly also of natural origin such as pig skin, have different properties in terms of spread behavior as human skin and leave no reproducible test conditions due to natural variations in the material properties such as weather conditions or violations of animal skin.

It is an object of the present invention to provide a skin substitute is available, which simulates reproducible human skin and can be simulated on the various methods for testing of raw materials to the skin.

Description of the Invention

The present invention is an apparatus for testing of cosmetic and pharmaceutical raw materials including a membrane which is stretched over a container containing a hydrogel as a supporting base for the membrane.

membrane

The membrane which is stretched over the hydrogel as a supporting base, are preferably suitable collagen membranes as they are also used in the food industry. It is preferable that this is collagen membranes that can be used as sausage skins. Examples are here membranes of Naturin that are represented by the names EWD-casing, COFFI film or Classic Casing in the market.

Should be present different surface textures such as lipophilicity, surface roughness, surface tension for the tests performed on the membranes tests, they can by the selection of the membrane, by the choice and concentration of the gel former, on the use of humectants in different concentrations, or by the application of sebum lipids to the membrane surface can be achieved.

hydrogel

Hydrogel gels can be used which have been prepared with a variety of gelling agents. In a preferred embodiment, hydrogels based on agar-agar, gelatin, hydroxyethylcellulose or mixtures thereof. Particularly preferred are the hydrogels based on agar-agar, as these can also be conditioned at 32 ° C, as they do not melt.

container

As a container for the skin substitute are all inert container, preferably glass containers are suitable.

Due to their dimensions in particular petri dishes are used.

Humectants

In order to keep the gels and the applied thereto membranes damp and thus usable during the test series, the hydrogels humectants may be added. These prevent premature drying of the measuring device. Furthermore, the surface properties of the membrane can be deliberately influenced by the humectant. The preferred humectant is glycerin can be considered. With respect to the hydrogels, the humectants% are used in amounts of 0.1 to 25. Employed.

test methods

With the help of the skin model cosmetic and pharmaceutical raw materials can be tested before as the dermatological or toxicological safety has been detected. Thus, the diffusion of water vapor at the surface of the skin model can be determined before and after treatment with a raw material or a composition with the aid of a Tewameters TM210 from Courage & Khazaka, Cologne, but also corneometrische (determination of the hydration of the surface of the skin model, for example by means of a corneometer CM820 from Courage & Khazaka, Cologne) and friktiometrische measurements (for example with a Fritiometer® FR 700) are possible. Also, the spreading behavior of oils on the skin model can (after Zeidler, Fette Seifen Anstrichmittel, Vol. 87, no. 10, Dusseldorf, 1985) to be tested and quantified. It is also possible, the surface energy before and determine, after treatment with various raw materials or compositions (eg, using a system Kontaktwinkelmess- OCAH200 the company Dataphysics Instruments GmbH, Filderstadt, Germany).

Depending on the test method, certain combinations of membranes and hydrogels have proven particularly useful. To measure the transdermal water loss (TEWL) and the corneometry particularly the skin models suitable based on a gelatin or agar-agar hydrogel with EWD-casing.

Examples

Example 1: Composition and preparation of the Hautsubstituts

Figure imgf000005_0001

Water and glycerol were heated. Gelatin was for 5-10 min water mixture swollen with 50 g of glycerol.

Once the mixture had reached a temperature of 50 to 6O 0 C, the swollen gelatin was added, and stirred using a magnetic stirrer until the gelatin was completely dissolved.

Phenoxyethanol was added and cooled the gelatin mass. Once the mass had reached a temperature of about 40 to 50 ° C, it was poured into petri dishes, which were filled to the brim.

The gelling of the gelatin mass takes about 30 min to 120 min, depending on the temperature and relative humidity in the environment.

Meanwhile, the collagen membrane for about 15 minutes was. moistened in water. Once the gelatin mass had a sufficient gel strength, the humidified collagen membrane was freed of excess water, and then stretched taut over the gelatin surface. Under the surface, occurring bubbles have been removed.

The conditioning was achieved in a climatic chamber at 20 ° C and 80 to 90% relative humidity over 15-2Oh.

Claims

claims
1. An apparatus for testing of cosmetic and pharmaceutical raw materials including a
which is stretched over a container containing a hydrogel as a supporting base for the diaphragm membrane.
2. Device according to claim 1, characterized in that it is the membrane is a collagen membrane.
3. A device according to any one of claims 1 or 2, characterized in that the collagen membrane is a sausage skin is used.
4. Device according to one of claims 1 to 3, characterized in that the membrane with humectants or sebum lipids was modifed.
5. Device according to one of claims 1 to 4, characterized in that a hydrogel is selected from the group formed by agar-agar. Gelatin, hydroxyethylcellulose or mixtures thereof is used.
6. The device according to claim 4, characterized in that glycerine is used as humectant.
PCT/EP2008/010146 2007-12-08 2008-11-29 Skin model WO2009071251A3 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
DE102007059199.5 2007-12-08
DE200710059199 DE102007059199A1 (en) 2007-12-08 2007-12-08 skin model

Publications (2)

Publication Number Publication Date
WO2009071251A2 true true WO2009071251A2 (en) 2009-06-11
WO2009071251A3 true WO2009071251A3 (en) 2009-08-13

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2008/010146 WO2009071251A3 (en) 2007-12-08 2008-11-29 Skin model

Country Status (2)

Country Link
DE (1) DE102007059199A1 (en)
WO (1) WO2009071251A3 (en)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4603105A (en) * 1982-05-27 1986-07-29 Kaplan Donald A Efficient screening methods for low probability variants
US4801548A (en) * 1984-10-09 1989-01-31 Kobayashi Pharmaceutical Co., Ltd. Petri dish for cultivating bacteria and method of inspecting drug susceptibility
US5834005A (en) * 1992-02-24 1998-11-10 Encelle, Inc. Bioartificial devices and cellular matrices therefor

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2006230304A (en) * 2005-02-25 2006-09-07 Nihon Pharmaceutical Co Ltd Device for detecting microorganism and method for detecting microorganism
JP2006230315A (en) * 2005-02-25 2006-09-07 Nihon Pharmaceutical Co Ltd Device for detecting microorganism and method for detecting microorganism

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4603105A (en) * 1982-05-27 1986-07-29 Kaplan Donald A Efficient screening methods for low probability variants
US4801548A (en) * 1984-10-09 1989-01-31 Kobayashi Pharmaceutical Co., Ltd. Petri dish for cultivating bacteria and method of inspecting drug susceptibility
US5834005A (en) * 1992-02-24 1998-11-10 Encelle, Inc. Bioartificial devices and cellular matrices therefor

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
CHEN CHUN-HUNG ET AL: "Novel living cell sheet harvest system composed of thermoreversible methylcellulose hydrogels" BIOMACROMOLECULES, Bd. 7, Nr. 3, März 2006 (2006-03), Seiten 736-743, XP002530849 ISSN: 1525-7797 *
DATABASE WPI Week 200662 Thomson Scientific, London, GB; AN 2006-598712 XP002530851 & JP 2006 230304 A (NIPPON SEIYAKU KK) 7. September 2006 (2006-09-07) *
DATABASE WPI Week 200663 Thomson Scientific, London, GB; AN 2006-606748 XP002530850 & JP 2006 230315 A (NIPPON SEIYAKU KK) 7. September 2006 (2006-09-07) *

Also Published As

Publication number Publication date Type
WO2009071251A3 (en) 2009-08-13 application
DE102007059199A1 (en) 2009-06-10 application

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