WO2009066071A3 - Antisense oligonucleotides for the modulation of gene expression and methods relating to the use thereof - Google Patents

Antisense oligonucleotides for the modulation of gene expression and methods relating to the use thereof Download PDF

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Publication number
WO2009066071A3
WO2009066071A3 PCT/GB2008/003887 GB2008003887W WO2009066071A3 WO 2009066071 A3 WO2009066071 A3 WO 2009066071A3 GB 2008003887 W GB2008003887 W GB 2008003887W WO 2009066071 A3 WO2009066071 A3 WO 2009066071A3
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WO
WIPO (PCT)
Prior art keywords
modulation
gene expression
antisense oligonucleotides
methods relating
sirna molecules
Prior art date
Application number
PCT/GB2008/003887
Other languages
French (fr)
Other versions
WO2009066071A2 (en
Inventor
Simon Plyte
Alessandra Saccani
Saverio Minucci
Original Assignee
Congenia S R L
Cripps Joanna
Simon Plyte
Alessandra Saccani
Saverio Minucci
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Congenia S R L, Cripps Joanna, Simon Plyte, Alessandra Saccani, Saverio Minucci filed Critical Congenia S R L
Publication of WO2009066071A2 publication Critical patent/WO2009066071A2/en
Publication of WO2009066071A3 publication Critical patent/WO2009066071A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/11—Antisense
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/11—Antisense
    • C12N2310/111—Antisense spanning the whole gene, or a large part of it
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering N.A.

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

The invention relates to oligonucleotides, in particular siRNA molecules, for modulating the expression of p66 shc. Disclosed herein are specific siRNA molecules which target mammalian p66shc mRNA and can be used in modulation of a signal transduction pathway that regulates stress response and lifespan in mammalian cells.
PCT/GB2008/003887 2007-11-20 2008-11-19 Antisense oligonucleotides for the modulation of gene expression and methods relating to the use thereof WO2009066071A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB0722759A GB0722759D0 (en) 2007-11-20 2007-11-20 Antisense oligonucleotides for the modulation of gene expression and methods relating to the use thereof
GB0722759.8 2007-11-20

Publications (2)

Publication Number Publication Date
WO2009066071A2 WO2009066071A2 (en) 2009-05-28
WO2009066071A3 true WO2009066071A3 (en) 2009-07-16

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2008/003887 WO2009066071A2 (en) 2007-11-20 2008-11-19 Antisense oligonucleotides for the modulation of gene expression and methods relating to the use thereof

Country Status (2)

Country Link
GB (1) GB0722759D0 (en)
WO (1) WO2009066071A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102618640B (en) * 2012-03-14 2013-11-20 天津医科大学 Molecular diagnostic kit for detecting p52Shc/p66Shc gene expression pattern, and detection method and application thereof
CN106701830B (en) * 2016-12-07 2020-01-03 湖南人文科技学院 Pig embryo p66 knock-outshcMethod for gene

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000056886A1 (en) * 1999-03-22 2000-09-28 Cancer Research Ventures Limited MATERIALS AND METHODS RELATING TO MODULATION OF p66 EXPRESSION

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000056886A1 (en) * 1999-03-22 2000-09-28 Cancer Research Ventures Limited MATERIALS AND METHODS RELATING TO MODULATION OF p66 EXPRESSION

Non-Patent Citations (15)

* Cited by examiner, † Cited by third party
Title
CHINTAPALLI JANAKI ET AL: "Inhibition of wild-type p66ShcA in mesangial cells prevents glycooxidant-dependent FOXO3a regulation and promotes the survival phenotype.", AMERICAN JOURNAL OF PHYSIOLOGY. RENAL PHYSIOLOGY FEB 2007, vol. 292, no. 2, February 2007 (2007-02-01), pages F523 - F530, XP002524319, ISSN: 0363-6127 *
DATABASE PROBE [online] NCBI; 1 November 2007 (2007-11-01), "Small interfering RNA (siRNA) probe for Homo sapiens gene ubiquitin-conjugating enzyme E2L 3 (UBE2L3). Developed for RNA interference (RNAi). Reagent is available from Qiagen.", XP002524328, retrieved from NCBI Database accession no. Pr008811168.1 *
DATABASE PROBE [online] NCBI; 14 April 2007 (2007-04-14), "Small interfering RNA (siRNA) probe for Homo sapiens gene golgin 97 (GOLGA1). Has been used for RNA interference (RNAi).", XP002524325, retrieved from NCBI Database accession no. Pr008693119.1 *
DATABASE PROBE [online] NCBI; 14 July 2007 (2007-07-14), "Small hairpin RNA (shRNA) probe TRCN0000009868 for Homo sapiens gene SHC (Src homology 2 domain containing) transforming protein 1 (SHC1). Developed for use in the TRC project for RNA interference (RNAi). Reagent is available from Sigma-Aldrich.", XP002524326, retrieved from NCBI Database accession no. PR008658045.1 *
DATABASE PROBE [online] NCBI; 17 January 2007 (2007-01-17), "Small interfering RNA (siRNA) probe for Homo sapiens gene cadherin 13 (CDH13). Has been used for RNA interference (RNAi).", XP002524327, retrieved from NCBI Database accession no. Pr008640206.1 *
DATABASE PROBE [online] NCBI; 8 July 2006 (2006-07-08), "Small interfering RNA (siRNA) probe for Homo sapiens gene SHC (Src homology 2 domain containing) transforming protein 1 (SHC1). Has been used for RNA interference (RNAi).", XP002524324, retrieved from NCBI Database accession no. Pr123315.1 *
KISIELOW MALGORZATA ET AL: "Isoform-specific knockdown and expression of adaptor protein ShcA using small interfering RNA.", THE BIOCHEMICAL JOURNAL 1 APR 2002, vol. 363, no. Pt 1, 1 April 2002 (2002-04-01), pages 1 - 5, XP002403515, ISSN: 0264-6021 *
NEMOTO S ET AL: "The mammalian longevity-associated gene product p66<shc> regulates mitochondrial metabolism", JOURNAL OF BIOLOGICAL CHEMISTRY 20060414 AMERICAN SOCIETY FOR BIOCHEMISTRY AND MOLECULAR BIOLOGY INC. US, vol. 281, no. 15, 14 April 2006 (2006-04-14), pages 10555 - 10560, XP002524323 *
SANSONE PASQUALE ET AL: "p66Shc/Notch-3 interplay controls self-renewal and hypoxia survival in human stem/progenitor cells of the mammary gland expanded in vitro as mammospheres.", STEM CELLS (DAYTON, OHIO) MAR 2007, vol. 25, no. 3, March 2007 (2007-03-01), pages 807 - 815, XP002524320, ISSN: 1066-5099 *
SANTEN R J ET AL: "Long-term estradiol deprivation in breast cancer cells up-regulates growth factor signaling and enhances estrogen sensitivity.", ENDOCRINE-RELATED CANCER JUL 2005, vol. 12 Suppl 1, July 2005 (2005-07-01), pages S61 - S73, XP002524514, ISSN: 1351-0088 *
SONG ROBERT X ET AL: "The role of Shc and insulin-like growth factor 1 receptor in mediating the translocation of estrogen receptor alpha to the plasma membrane.", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 17 FEB 2004, vol. 101, no. 7, 17 February 2004 (2004-02-17), pages 2076 - 2081, XP002524321, ISSN: 0027-8424 *
TIBERI L ET AL: "p66<Shc> gene has a pro-apoptotic role in human cell lines and it is activated by a p53-independent pathway", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, ACADEMIC PRESS INC. ORLANDO, FL, US, vol. 342, no. 2, 7 April 2006 (2006-04-07), pages 503 - 508, XP024923700, ISSN: 0006-291X, [retrieved on 20060407] *
VEERAMANI SURESH ET AL: "EXPRESSION OF P66(SHC) PROTEIN CORRELATES WITH PROLIFERATION OF HUMAN PROSTATE CANCER CELLS", ONCOGENE, NATURE PUBLISHING GROUP, GB BASINGSTOKE, HANTS, vol. 24, no. 48, 1 November 2005 (2005-11-01), pages 7203 - 7212, XP008075227, ISSN: 0950-9232 *
WU ZHIHAO ET AL: "Reduction of p66Shc suppresses oxidative damage in retinal pigmented epithelial cells and retina", JOURNAL OF CELLULAR PHYSIOLOGY, vol. 209, no. 3, December 2006 (2006-12-01), pages 996 - 1005, XP002524322, ISSN: 0021-9541 *
XIE ZHONGCONG ET AL: "RNA interference silencing of the adaptor molecules ShcC and Fe65 differentially affect amyloid precursor protein processing and Abeta generation.", THE JOURNAL OF BIOLOGICAL CHEMISTRY 16 FEB 2007, vol. 282, no. 7, 16 February 2007 (2007-02-16), pages 4318 - 4325, XP002524318, ISSN: 0021-9258 *

Also Published As

Publication number Publication date
WO2009066071A2 (en) 2009-05-28
GB0722759D0 (en) 2008-01-02

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