WO2009061935A2 - Procédés d'identification d'antagonistes du récepteur de nmda sûrs - Google Patents

Procédés d'identification d'antagonistes du récepteur de nmda sûrs Download PDF

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WO2009061935A2
WO2009061935A2 PCT/US2008/082660 US2008082660W WO2009061935A2 WO 2009061935 A2 WO2009061935 A2 WO 2009061935A2 US 2008082660 W US2008082660 W US 2008082660W WO 2009061935 A2 WO2009061935 A2 WO 2009061935A2
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compound
potency
disorder
pain
cells
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WO2009061935A3 (fr
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Raymond J. Dingledine
Stephen F. Traynelis
Dennis C. Liotta
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Emory University
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Priority to CN2008801240927A priority patent/CN101918832A/zh
Priority to CA2704475A priority patent/CA2704475A1/fr
Priority to AU2008323877A priority patent/AU2008323877A1/en
Priority to EP08847388A priority patent/EP2212694A4/fr
Priority to EA201070571A priority patent/EA201070571A1/ru
Priority to BRPI0820406-3A priority patent/BRPI0820406A2/pt
Priority to MX2010004971A priority patent/MX2010004971A/es
Publication of WO2009061935A2 publication Critical patent/WO2009061935A2/fr
Publication of WO2009061935A3 publication Critical patent/WO2009061935A3/fr
Priority to IL205432A priority patent/IL205432A0/en
Priority to US12/772,798 priority patent/US20100272648A1/en
Priority to ZA2010/03724A priority patent/ZA201003724B/en

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/04—Centrally acting analgesics, e.g. opioids
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/08—Antiepileptics; Anticonvulsants
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/14—Drugs for disorders of the nervous system for treating abnormal movements, e.g. chorea, dyskinesia
    • A61P25/16—Anti-Parkinson drugs
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • A61P9/10—Drugs for disorders of the cardiovascular system for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/84—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving inorganic compounds or pH
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
    • G01N2333/70571—Assays involving receptors, cell surface antigens or cell surface determinants for neuromediators, e.g. serotonin receptor, dopamine receptor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases
    • G01N2800/28—Neurological disorders
    • G01N2800/2835—Movement disorders, e.g. Parkinson, Huntington, Tourette
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases
    • G01N2800/28—Neurological disorders
    • G01N2800/2842—Pain, e.g. neuropathic pain, psychogenic pain
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases
    • G01N2800/28—Neurological disorders
    • G01N2800/2857—Seizure disorders; Epilepsy
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases
    • G01N2800/28—Neurological disorders
    • G01N2800/2871—Cerebrovascular disorders, e.g. stroke, cerebral infarct, cerebral haemorrhage, transient ischemic event

Definitions

  • This invention is in the area of improved methods for the selection of safe and effective pH dependent N-methyl D-aspartate receptor antagonists to be used before, during or after a pH-lowering event as a means to minimize or prevent tissue damage.
  • Nerve cells transmit signals from the environment to the central nervous system (CNS), among different regions of the CNS, and from the CNS back to other organs (i.e., the periphery). This signal transmission is mediated primarily by small molecules called neurotransmitters.
  • neurotransmitters can be classified as either excitatory or inhibitory. Excitatory neurotransmitters increase and inhibitory neurotransmitters decrease the activity (e.g., the firing rate) of the signal-receiving (i.e., postsynaptic) neuron. Neurons differ in their abilities to recognize, integrate, and pass on the signals conveyed by neurotransmitters. For example, some neurons continually fire at a certain rate and thus can be either excited or inhibited in response to environmental changes.
  • glutamate also called glutamic acid
  • Glutamate As an important signaling molecule has been recognized only within the past two decades.
  • Glutamate is an amino acid. Glutamate, as other amino acids, is present throughout the brain in relatively high concentrations. Consequently, researchers initially thought that glutamate was primarily an intermediate metabolic product of many cellular reactions unrelated to neuronal signal transmission and thus did not interpret its presence in neurons as evidence of a potential role as a neurotransmitter. The first indications of glutamate's excitatory function in the brain emerged in the 1950's, however, these findings were initially dismissed because glutamate application to neurons elicited excitatory responses in virtually every brain area examined, suggesting that this excitation was not a specific response. Only later did scientists recognize that the observed effects of glutamate were indeed valid because they could be attributed to the activation of excitatory receptors present throughout the CNS.
  • glutamate receptors i.e. proteins on the surface of neurons that specifically bind glutamate secreted by other neurons and thereby initiate the events that lead to the excitation of the postsynaptic neuron.
  • the identification of these glutamate receptors underscored glutamate's importance as an excitatory neurotransmitter.
  • NMDA N-methyl-D-aspartate
  • AMPA al ⁇ ha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid
  • kainate receptors There are also three groups of metabotropic, G protein-coupled glutamate receptors (mGluR) that modify neuronal and glial excitability through G protein subunits acting on membrane ion channels and second messengers such as diacylglycerol and cAMP.
  • mGluR G protein-coupled glutamate receptors
  • Glutamate is essential for normal brain function. Glutamate plays a primary role in the control of cognition, motor function, synaptic plasticity, learning and memory. High levels of endogenous glutamate, through its overactivation of NMDA, AMPA or mGluRl receptors, can contribute to brain damage. Examples of brain damage associated with excess glutamate or excitotoxicity are seen after status epilepticus, cerebral ischemia and traumatic brain injury. Excitotoxicity (e.g., toxicity caused by the overactivation of glutamate receptors) also contributes to chronic neurodegeneration in such disorders as Parkinson's disease, Alzheimer's disease, amyotrophic lateral sclerosis and Huntington's chorea.
  • NMDA and AMPA receptor antagonists protect against acute brain damage and delayed behavioral deficits.
  • Other clinical conditions that may respond to drugs acting on glutamatergic transmission include epilepsy, amnesia, anxiety, hyperalgesia and psychosis (Meldrum BS. J Nutr. 2000 Apr;130(4S Sup ⁇ l): 1007S-15S).
  • NMDA receptors are composed of NRl, NR2 (A, B, C, and D), and NR3 (A and B) subunits, which determine the functional properties of native NMDA receptors. Expression of the NRl subunit alone does not produce a functional receptor; co-expression of one or more NR2 subunits is required to form functional channels.
  • the NMDA receptor requires a co-agonist, glycine, to bind to allow the receptor to function.
  • the glycine binding site is found on the NRl subunit, whereas the glutamate binding site is found on NR2 subunits.
  • the NR3 subunit also binds glycine.
  • the NR2B subunit also possesses a binding site for spermine-like polyamines, which are regulatory molecules that modulate the functioning of the NMDA receptor.
  • NMDA receptors are largely inactive. This is due to a voltage- dependent block of the channel pore by magnesium ions, preventing ion flow through it. Depolarization releases channel block and permits activated NMDA receptors to carry ionic current across the postsynaptic membrane.
  • NMDA receptors are permeable to calcium ions as well as other ions.
  • the NMDA receptor is modulated by a number of endogenous and exogenous compounds. Likewise, sodium, potassium and calcium ions not only pass through the NMDA receptor channel but also modulate the activity of NMDA receptors. Zinc blocks the NMDA current through NR2A-containing receptors in a noncompetitive, high affinity and voltage-independent manner. It has also been demonstrated that polyamines do not directly activate NMDA receptors, but instead act to potentiate or inhibit glutamate-mediated responses.
  • NMDA receptor blockers are effective in limiting the volume of damaged brain tissue in experimental models of stroke and traumatic brain injury (Choi, D. (1998), Mount Sinai J Med 65: 133-138; Dirnagle et al. (1999) Tr. Neurosci. 22:391-397; Obrenovitch, T.P. and Urenjak, J. (1997) J Neurotrauma 14:677 ).
  • NMDA receptor antagonists have been tested in early clinical trials for stroke. Stroke is the third leading cause of death in the United States and the most common cause of adult disability. An ischemic stroke occurs when a cerebral vessel occludes, obstructing blood flow to a portion of the brain.
  • TPA tissue plasminogen activator
  • Neuroprotective agents have generated as much interest as thrombolytic therapies (http://www.emedicine.com/neuro/topic488.htm, Lutsep & Clark "Neuroprotective Agents in Stroke", April 30, 2004), however, have not yet been approved for human therapy.
  • NMDA N- methyl-D-aspartate
  • Epilepsy has long been considered a potential therapeutic target for glutamate receptor antagonists. Indeed, the common anticonvulsant valproate, in therapeutic concentrations, may act as an anticonvulsant partly by blocking AMPA receptors.
  • NMDA receptor antagonists are known to be anticonvulsant in many experimental models of epilepsy (Bradford (1995) Progress in Neurobiology 47:477-51 1; McNamara, J.O. (2001) Drugs effective in the therapy of the epilepsies. In Goodman & Gliman's: The pharmaco logical basis of therapeutics [Eds. J.G. Hardman and L.E. Limbird] McGraw Hill, New York).
  • NMDA receptor antagonists may be beneficial in the treatment of chronic pain.
  • Chronic pain such as that due to injury of peripheral or central nerves, has often proved very difficult to treat, even with opioids.
  • Treatment of chronic pain with ketamine and amantadine has proven beneficial, and it is believed that the analgesic effects of ketamine and amantadine are mediated by block of NMDA receptors.
  • systemic administration of amantadine or ketamine substantially reduces the intensity of trauma- induced neuropathic pain. Small-scale double blind, randomized clinical trials corroborated that amantadine could significantly reduce neuropathic pain in cancer patients (Pud et al.
  • NMDA receptor antagonists can also be beneficial in the treatment of Parkinson's Disease (Blandini and Greenamyre (1998), Fundam Clin Pharmacol 12:4-12).
  • the antiparkinsonian drug, amantadine is an NMDA receptor channel blocker (Blanpied et al. (1997), J Neurophys 77:309-323).
  • Amantadine is seldom used alone due to limited efficacy.
  • a small-scale clinical trial demonstrated the value of amantadine as add-on therapy with L-DOPA. Amantadine reduced the severity of dyskinesias by 60% in these patients without reducing the antiparkinsonian effect of L-DOPA itself (Verhagen Metman et al. (1998), Neurology 50:1323-1326).
  • NMDA receptor antagonist CP- 101,606
  • CP- 101,606 potentiated the relief of Parkinson's symptoms by L-DOPA in a monkey model (Steece-Collier et al., (2000) Exper. Neurol, 163:239-243).
  • NMDA receptor antagonists may in addition be beneficial in the treatment of brain cancer. Rapidly-growing brain gliomas can kill adjacent neurons by secreting glutamate and overactivating NMDA receptors such that the dying neurons make room for the growing tumor, and may release cellular components that stimulate tumor growth. Studies show NMDA receptor antagonists can reduce the rate of tumor growth in vivo as well as in some in vitro models. (Takano, T., et al. (2001), Nature Medicine 7:1010-1015; Rothstein, J.D. and Bren, H. (2001) Nature Medicine 7:994-995; Rzeski, W., et al. (2001), Proc. Nat'l Acad. Sci 98:6372).
  • NMDA-receptor antagonists might be useful to treat a number of very challenging disorders, to date, dose-limiting side effects have thus far prevented clinical use of NMDA receptor antagonists for these conditions.
  • the first three generations of NMDA receptor antagonists channel blockers, competitive blockers of the glutamate or glycine agonist sites, and noncompetitive allosteric antagonists
  • NMDA receptor antagonists channel blockers, competitive blockers of the glutamate or glycine agonist sites, and noncompetitive allosteric antagonists
  • the cardiovascular side effects hypertension
  • undesirable effects on memory and attention can also result from administration of NMDA antagonists.
  • NMDA receptor antagonists such as ketamine can also produce a psychotic state in humans reminiscent of schizophrenic symptoms (Krystal et al. (1994), Arch Gen Psychiatry 51 :199- 214). Additionally, ataxia, cognitive deficits, motor impairment, agitation, confusion, dizziness and hypothermia have all resulted from administration of NMDA antagonists. Thus, despite the tremendous potential for glutamate antagonists to treat many serious diseases, the severity of the side effects have caused many to abandon hope that a well- tolerated NMDA receptor antagonist could be developed (Hoyte L.
  • NMDA receptors Two of the most prevalent subtypes of NMDA receptors have the unusual property of being normally inhibited by protons by about 50% at physiological pH (Traynelis, S.F. and Cull-Candy, S.G. (1990) Nature 345:347).
  • the inhibition of NMDA receptors by protons is controlled by the NR2B subunit and NR2A subunit, as well as alternative exon splicing in the NRl subunit (Traynelis et al. (1998), J Neurosci 18:6163-6175).
  • the extracellular pH is highly dynamic in mammalian brain, and influences the function of a multitude of biochemical processes and proteins, including glutamate receptor function.
  • the pH-sensitivity of the NMDA receptor has received increasing attention for at least two reasons. First, the IC50 value for proton inhibition of pH 7.4 places the receptor under tonic inhibition at physiological pH.
  • Such feedback inhibition might also delay the contribution of NMDA receptor activation to ischemic cell death to a point in time at which the pH gradient has recovered before glutamate has been removed from the interstitial space.
  • the pH sensitivity of glutamate uptake is consistent with this latter possibility (Billups and Attwell (1996), Nature (Lond) 379:171-173) which may enhance the opportunity for post-insult treatment of, for example, stroke with NMDA receptor antagonists (Tombaugh and Sapolsky (1993), J Neurochem 61 :793-803).
  • tissue extracellular pH is typically more acidic than cerebrospinal fluid due to regulation of protons as well as active and passive movement of metabolites.
  • Dynamic activity-dependent multiphasic acid and alkaline changes in extracellular pH have been known to occur for almost two decades. These changes have been described in a wide range of preparations and brain regions. They involve multiple molecular mechanisms, which include metabolic changes, lactic acid secretion, bicarbonate efflux through anionic channels, Na+/H+ and Ca 2+ /H + exchange, and proton release from acidified vesicles.
  • Neuropathic pain is associated with pH changes in the spinal cord.
  • single electrical stimulation of isolated spinal cord from rat pups produce an alkaline shift of 0.05 pH units, and a 0.1 pH unit shift following 10 Hz stimulation.
  • An acidification followed the cessation of stimuli, and this acidification is larger in older animals (Jendelova & Sykova (1991) GHa 4: 56-63).
  • 30-40 Hz stimulation of the dorsal root in frog produced in vivo a transient extracellular acidification reaching a maximum ceiling of 0.25 pH unit reduction in the lower dorsal horn.
  • Extracellular pH changes increased with stimulus intensity and frequency (Chvatal et al. (1988) Physiol Bohemoslov 37: 203-212).
  • High frequency nerve stimulation produced an alkaline pH shift followed by a dominating 0.2 pH unit acid shift (Sykova & Svoboda (1990) Brain Res 512: 181-189).
  • increased firing of pain fibers can cause a decrease in pH (acidification) of the dorsal horn of the spinal cord. This acidification could lead to an increased potency of pH dependent blockers in the region, making them useful in treatment of chonic nerve injury or chronic pain syndromes.
  • Subthalamic neurons are overactive in Parkinson's disease and this may result in a lower local pH. Such a reduced pH would increase potency of pH-sensitive antagonists in this region.
  • High frequency stimulation of brain slices gives an initial acidification followed by an alkalinization, followed by a slow acidification (See, for example, Chesler (1990)Prog Neurobiol 34: 401-427, Chesler & Kaila (1992)Tr Neurosci 15: 396-402, and Kaila & Chesler (1998) "Activity evoked changes in extracellular pH” in pH and Brain function (eds Kaila and Ransom). Wiley-Liss, New York).
  • NMDA antagonists are anticonvulsant, and thus epilepsy represents a target in which pH sensitive NMDA antagonists could effectively act as anticonvulsants while remaining inactive outside the spatial and temporal confines of the seizure.
  • Electrographic seizures in a wide range of preparations have been shown to cause a change in extracellular pH. For example, up to a 0.2-0.36 drop in pH can occur in cat fascia dentata or rat hippocampal CAl or dentate during an electrically or chemically evoked seizure. Deeper drops in pH approaching 0.5 can occur under hypoxic conditions.
  • Spreading depression is a term used to describe a slowly moving wave of electrical inactivity that occurs following a number of traumatic insults to brain tissue. Spreading depression can occur during a concussion or migraine. Acidic pH changes occur with spreading depression. Systemic alkalosis can occur with and reduction in overall carbon dioxide content (hypocapnia) through, for example, hyperventilation. Systemic acidosis can occur with an increase in blood carbon dioxide (hypercapnia) during respiratory distress or conditions that impair gas exchange or lung function. Diabetic ketoacidosis and lactic acidosis represent three of the most serious acute complications of diabetes and can result in brain acidification. Further, fetal asphyxia during parturition occurs in 25 per 1000 births at term. It involves hypoxia and brain damage that is similar but not identical to ischemia.
  • Traynelis et al. (1995 Science 268:873) reported for the first time that the small molecule spermine could modulate NMDA receptor function through relief of proton inhibition.
  • Spermine a polyamine, shifts the pKa of the proton sensor to acidic values, reducing the degree of tonic inhibition at physiological pH, which appears as a potentiation of function (Traynelis et al. (1995), Science 268:873-876; Kumamoto, E (1996), Magnes Res 9(4):317-327).
  • Ifenprodil is neuroprotective in animal models of focal cerebral ischemia (Gotti et al. (1988), J Pharmacol Exp Ther 247: 1211-1221; Dogan et al. (1997), J Neurosurg 87(6):921- 926). Ifenprodil has been shown to be neuroprotective in mammals after middle cerebral artery occlusion. Dogan et al. reported a 22% decrease in infarct volume in rats, whereas Gotti et al. reported a 42% decrease infarct volume at the highest dose tested in cats. Gotti et al.
  • SL 82.0715 an ifenprodil derivative, produced a 36-48% decrease in infarct volume at the highest dose tested in cats and rats.
  • ifenprodil and several of its analogs including eliprodil and haloperidol (Lynch and Gallagher (1996), J Pharmacol Exp Ther 279:154-161; Brimecombe et al. (1998), J Pharmacol Exp Ther 286(2): 627-634), block certain serotonin receptors and calcium channels in addition to NMDA receptors, limiting their clinical usefulness (Fletcher et al.
  • NMDA antagonists In addition to these allosteric modulators, other NMDA antagonists have been shown to produce neuroprotective effects in animal models of focal ischemia (Gill et al (1994) Cerebrovascular and Brain Metabolism Reviews 6: 225-256). These NMDA antagonists fall into three functional classes: competitive blockers of the glutamate binding site, competitive blockers of the glycine binding site and channel blockers, which produce toxic side effects or exhibit limited efficacy in humans.
  • the competitive NMDA antagonists of the glutamate site such as, selfotel, D- CPPene (SDZ EAA 494) and AR-Rl 5896AR (ARL 15896AR), cause toxic side effects including, agitation, hallucination, confusion and stupor (Davis et al. (2000), Stroke 31(2):347-354; Diener et al. (2002), J Neurol 249(5):561-568); paranoia and delirium (Grotta et al. (1995), J Intern Med 237:89-94); psychotomimetic-like symptoms (Loscher et al.
  • glycine site antagonists such as HA-966, L-701,324, d-cycloserine, CGP- 40116, and ACEA 1021 produce toxic side effects, including significant memory impairment and motor impairment (Wlaz, P (1998), Brain Res Bull 46(6):535-540).
  • NMDA receptor channel blockers including MK-801 and ketamine, can produce toxic side effects, such as psychosis-like (Hoffman, DC (1992), J Neural Transm Gen Sect 89: 1-10); cognitive deficits (decrements in free recall, recognition memory, and attention; Malhotra et al (1996), Neuropsychopharmacology 14:301-307); schizophrenia-like symptoms (Krystal et al (1994), Arch Gen Psychiatry 51 :199-214; Lahti et al. (2001), Neuropsychopharmacology 25 :455-467).
  • toxic side effects such as psychosis-like (Hoffman, DC (1992), J Neural Transm Gen Sect 89: 1-10); cognitive deficits (decrements in free recall, recognition memory, and attention; Malhotra et al (1996), Neuropsychopharmacology 14:301-307); schizophrenia-like symptoms (Krystal et al (1994), Arch Gen Psychiatry 51 :199-214; Lah
  • WO 02/072542 to Emory University describes a class of pH-dependent NMDA receptor antagonists that exhibit pH sensitivity tested in vitro using an oocyte assay and in an experimental model of epilepsy.
  • the in vitro data using Xenopus oocytes was subject to wide variations in measured ICso's for selected compounds, which limited accurate selection of the optimal, or lead, compound.
  • the assays were limited to cell-based screens, they lacked the ability to assess whether there is a sufficiently large drop in pH in affected ischemic tissue in vivo to observe a substantial effect caused by the pH-dependent antagonist.
  • WO 06/023957 to Emory University describes processes for the identification of a compound that is useful to treat ischemic injury by.
  • the drug must not significantly affect normal functioning of glutamate neurotransmission, yet provide an effective blockade of the glutamate system during pathological conditions thereby avoiding the toxic side effects. It has been challenging to predict whether or not pH-dependent selective NMDA antagonists that demonstrate a greater affinity for the NMDA receptor at a lower pH in vitro would also display a sufficient response in vivo to provide a commercial drug. While pH-dependant NMDA receptor anatagonists have been developed, the appropriate properties of these drugs have not yet been determined to accurately establish successful parameters for selection of a drug for human clinical use.
  • An improved process for identification of an improved NMDA-receptor antagonist for the treatment or prevention of a disorder that lowers the pH in a region of affected tissue for superior human clinical performance uses cells that express human NMDA receptors to assess the pH potency boost of a compound, i.e. the difference between the efficacy of a compound in inhibiting NMDA receptor activation at physiological pH versus pathogenic pH, in vitro.
  • This process identifies compounds with improved safety and efficacy profiles over previously known techniques.
  • the inventors have surprisingly discovered that pH potency boost assessed in a cell that expresses human NMDA receptors provides an improved method for the selection of effective and safe NMDA receptor antagonists, when compared to using cells expressing NMDA receptors from other mammals.
  • pH potency boost obtained from non- human NMDA receptors is not a reliable predictor of the pH potency boost obtained from human NMDA receptors.
  • the safety of the NMDA receptor antagonists arises from the lack of efficacy of the compound at physiological pH. Therefore, the ideal compounds are those that have very low efficacy at physiological pH, but are highly effective in pathological conditions with lowered pH.
  • NMDA receptor antagonists for the treatment or prevention of a human disorder that lowers the pH in a region of affected tissue.
  • Such disorders include, but are not limited to, neuropathic pain, ischemia, Parkinsons disease, epilepsy and traumatic brain injuries.
  • a process is provided to identify a compound that is useful to treat or prevent a disorder that lowers the pH in a region of affected tissue comprising assessing the difference in potency of the compound at physiological pH versus disorder- induced pH (for example, IC50 at physiologic pH / IC50 at disorder induced low pH) in a cell that expresses a human NMDA receptor.
  • the assessment of potency boost can include measuring an IC 5 0 of a compound at physiological pH and at disorder-induced pH (the "potency boost") until a 95% confidence interval for the potency boost does not change more than 15% with the addition of a new experiment, wherein the measurements are repeated at least 5 times.
  • the process further comprises identifying compounds with a potency boost in such cells of at least 5.
  • the potency boost of the compounds in these cells is at least 2 more than, or at least 3 more than, or at least 4 more than, or at least 5 more than, or at least 6 more than, or at least 7 more than, or at least 8 more than, or at least 9 more than, or at least 10 more than the potency boost of the same compounds when tested in a cell that expresses a non-human NMDA receptor.
  • the potency boost of the compound is less than 100 or less than 50 more than a potency boost of the same compound when tested in a cell that expresses a non-human NMDA receptor.
  • the non-human NMDA receptor is a rat NMDA receptor.
  • the affected tissue is selected from brain tissue, tissue damaged by an ischemia, tissue affected by pain and in particular by neuropathic pain, and tissue affected by traumatic brain injuries.
  • the 95% confidence interval does not change more than 10%, more than 8%, more than 5%, more than 4%, more than 3% or more than 2% with the addition of a new experiment.
  • the potency boost experiment is repeated 5 times, at least 6 times, at least 7 times, at least 8 times, at least 9 times, or at least 10 times.
  • a process to identify a compound that is useful to treat or prevent a pain disorder in a region of affected tissue comprising: (i) assessing potency of a compound at inhibiting a human NMDA receptor at physiological pH versus disorder- induced pH in a cell that expresses human NMDA receptors; (ii) testing the compound in vivo and measuring the effect of the compound on a pain threshold; and (iii) selecting a compound that has a potency boost of at least 5 according to step (i) and is associated with at least a 2-fold increase in pain threshold according to step (ii).
  • the potency boost can be measured by measuring an IC 50 of a compound at physiological pH and at disorder-induced pH (the "potency boost") until a 95% confidence interval for the potency boost does not change more than 15% with the addition of a new experiment, wherein the measurements are repeated at least 5 times.
  • the potency boost is measured at least 12 times.
  • the pain threshold is measured until a 95% confidence interval does not change more than 5% with the addition of a new experiment. In specific embodiments, the pain threshold is measure at least 12 times.
  • a 95% confidence interval of the potency boost obtained in step (i) does not change more than 15%, more than 10%, more than 8%, more than 5%, more than 4%, more than 3% O ⁇ more than 2% with the addition of a new experiment.
  • the potency boost experiment of step (i) is repeated 5 times, at least 6 times, at least 7 times, at least 8 times, at least 9 times, or at least 10 times.
  • the pain threshold can be measured in animal models of pain, and in particular in animal models of neuropathic pain.
  • a 95% confidence interval of the pain threshold obtained in step (ii) does not change more than 15%, more than 10%, more than 8%, more than 5%, more than 4%, more than 3% or more than 2% with the addition of a new experiment.
  • the 95% confidence interval obtain in step (ii) does not change more than 5%.
  • the disorder lowers the pH in the affected tissue.
  • the disorder that lowers the pH in a region of affected tissue is a pain disorder, and in particular can be neuropathic pain.
  • a process to identify a compound that is useful to treat or prevent neuropathic pain comprising: (i) assessing the potency boost of the compound at physiological pH versus disorder-induced low pH in a cell that expresses human NMDA receptors by repeating the potency boost experiment at least 5 times and until the 95% confidence interval does not change more than 15% with the addition of a new experiment; (ii) testing the compound in an animal model of neuropathic pain and measuring the effect of the compound on the increase in pain threshold by repeating the experiment at least 12 times and until the 95% confidence interval does not change more than 5% with the addition of a new experiment; (iii) selecting a compound that has a potency boost of at least 5 according to step (i) and is associated with at least a 2-fold increase in pain threshold according to step (ii).
  • the compound exhibits a potency boost of at least 6, 7, 8, 9, 10, 15 or 20 according to step (i) and at least a 2-fold, 3-fold, 4-fold, 5-fold, 6-fold, 7-fold, 8- fold, 9-fold, 10-fold increase in pain threshold according to step (ii).
  • methods to attenuate the progression of an ischemic or excitotoxic cascade associated with a drop in pH by administering a compound selected according to the processes or methods described herein are provided.
  • methods are provided to decrease infarct volume associated with a drop in pH by administering a compound selected according to the processes or methods described herein.
  • a method is provided to decrease cell death associated with a drop in pH by administering a compound selected according to the processes or methods described herein.
  • methods are provided to decrease behavioral deficits associated with an ischemic event associated with a drop in pH by administering a compound selected according to the processes or methods described herein.
  • methods are provided to treat patients with ischemic injury or prevent or treat the neuronal toxicity associated with ischemic injury by administering a compound selected according to the methods or processes described herein.
  • methods are provided to treat the following diseases or neurological conditions, including, but not limited to: Parkinson's disease, chronic nerve injury, chronic pain syndromes, such as, but not limited to diabetic neuropathy, ischemia, ischemia following transient or permanent vessel occlusion, seizures, "spreading depression", hypocapnia, hypercapnia, diabetic ketoacidosis, fetal asphyxia, cognitive deficit after bypass surgery, vasospasm after subarachnoid hemorrhage, spinal cord injury, traumatic brain injury, status epilepticus, epilepsy, hypoxia, perinatal hypoxia, concussion, migraine, hypocapnia, hyperventilation, lactic acidosis, fetal asphyxia during parturition, brain gliomas, and/or retinopathies
  • Parkinson's disease chronic nerve
  • compounds selected according to the methods or processes described herein can be used prophylactically to prevent or protect against such diseases or neurological conditions, for example, in patients with a predisposition for an ischemic event, such as a genetic predisposition, or in patients that exhibit vasospasms, or in patients that have undergone cardiac bypass surgery.
  • a predisposition for an ischemic event such as a genetic predisposition
  • vasospasms or in patients that have undergone cardiac bypass surgery.
  • Figure 1 is a graph of the comparison of the in vitro potency boost at pH 6.9 vs 7.6 versus tissue infarct volume reductions for a selection of NMDA receptor antagonists and control treatments in C57B1/6 mice following a transient or permanent focal ischemic event.
  • Drug was applied intracerebroventricularly (ICV; 1 microliter of 0.5 mM; solid squares) or by intraperitoneal injection (IP, solid circles; NP93-4, 30 mg/kg; NP93-5, 10-30 mg/kg; NP93-40, 10-30 mg/kg; NP93-8, 30 mg/kg; NP93-31, 3 mg/kg).
  • ICV intracerebroventricularly
  • IP intraperitoneal injection
  • Infarct volume in drug-treated animals was directly measured and expressed as a percent of the infarct volume in vehicle injected control mice.
  • Open symbols show the reduction in infarct volume by administration of CNSl 102 (CN, aptiganel or Cerestat, Dawson et al., 2001), dextromethorphan (DM, Steinberg et al., 1995), dextrorphan (DX; Steinberg et al., 1995), levomethorphan (LM; Steinberg et al., 1995), (S) ketamine (KT; Proescholdt et al., 2001), memantine (MM; Culmsee et al. 2004), ifenprodil (IF, Dawson et al.
  • Percent reduction in infarct was calculated from the ratio of the infarct volume for all compounds except ketamine and 7-Cl-kynurenic acid, for which the percent reduction in neuronal density was measured.
  • the pH boosts for ifenprodil and CPl 01, 606 were determined from the literature (Mott et al., 1998).
  • the potency boosts for the inhibition of NR1/NR2B containing NMDA receptors at pH 6.9 vs 7.6 were calculated as described herein, except competitive antagonists, which were evaluated in 2 experiments (see Table 3 below).
  • the drugs that fall within the grey shadowed area are those with superior in vivo safety and efficacy potential.
  • Figure 2 is a graph comparing in vitro potency boost of selected compounds at pH 6.9 vs 7.6 versus tissue infarct volume protection when the test drug was applied intracerebroventricularly (ICV; solid squares).
  • the grey shadowed area indicates the area which defines the identified bounds of the criteria for superior drug performance.
  • Figure 3 is a comparison of in vitro potency boost of selected compoundsat pH 6.9 vs 7.6 versus tissue infarct volume protection when the test drug was applied by intraperitoneal injection (IP, solid circles).
  • IP intraperitoneal injection
  • Figure 4 is a comparison of in vitro potency boost at pH 6.9 vs 7.6 versus tissue infarct volume of selected compounds.
  • the grey shadowed area indicates the area which defines the identified bounds of the criteria for superior drug performance.
  • the right panel shows comparison for NR1/NR2A and the left panel shows comparison for NR1/NR2B.
  • Figure 5 illustrates the effects of Compounds 93-31 and (+)MK-801 on locomotor activity of rats, quantified as light beam breaks counted by a computer during a 2 hour period following 1 hour habituation.
  • the Locomotor Activity Index is the total number of beam breaks during the trial divided by 1000.
  • Figure 6 illustrates that the injured paw showed substantial allodynia in the animal model of neuropathic pain.
  • Figure 9 illustrates that Compound 93-31 (100 mg/kg) administered i.p. attenuated mechanical allodynia in the Spinal Nerve Ligation (SNL) model in the rat.
  • Treatment with the compound 93-31 (100 mg/kg i.p.) generated observable analgesia at 30 and 60 min following its administration.
  • Statistical analysis of the vehicle group in this study indicated there was no significant difference in von Frey threshold between baseline and at 60 120 and 240 minute time point (Friedman two-way ANOVA).
  • Figure 10 shows that Compound 93-31 (100 mg/kg) administered i.p. attenuated mechanical allodynia in SNL rat.
  • I.P. administration of Compound 93-31 test compound (100 mg/kg) reduced mechanical allodynia. Shown are the mean ⁇ SEM (n 10-12) von Frey thresholds in the injured paw of animals treated with vehicle, gabapentin (reference compound) or 30 and 100 mg/kg doses of Compound 93-31 administered i.p.
  • Post-hoc analysis (Dunn's test) showed significant pair-wise differences between Compound 93-31 (100 mg/kg ) and vehicle groups at 30 and 60 minute (p ⁇ 0.01).
  • the effect of gabapentin at 60, 120 and 240 minutes was also significant (p ⁇ 0.001, p ⁇ 0.01, and p ⁇ 0.01 respectively).
  • Figure 11 is a comparison of the in vitro potency boost at pH 6.9 vs 7.6 versus fold increase in pain threshold in a rodent spinal nerve ligation model. Potency boosts were determined for each compound as decribed herein. The pain threshold was measured after administration of Compound 93-31. The pain threshold values were previously reported for IF (ifenprodil, De Vry et al, Eur J Pharmacol 491:137-148, 2004), K (ketamine, Chaplan et al. JPET 280:829-838 1997), CP (CPlOl, 606, Boyce et al. Neduropharmacol 38:611-623, 1999), MK (MK801, Chaplan et al.
  • IF ifenprodil, De Vry et al, Eur J Pharmacol 491:137-148, 2004
  • K ketamine, Chaplan et al. JPET 280:829-838 1997)
  • CP CPlOl, 606, Boyce
  • the grey shadowed area indicates the area which defines the identified bounds of the criteria for superior drag performance.
  • An improved process for selection of a safe and effective NMDA-receptor antagonist for the treatment or prevention of a disorder that lowers the pH in a region of affected tissue for superior human clinical performance is provided. This has been accomplished by the use of cells that express human NMDA receptors to assess the pH potency boost of a compound in vitro. The inventors have surprisingly discovered that for the processes described herein the pH potency boost assessed in a cell that expresses human NMDA receptors is an improved method for the selection of safe NMDA receptor antagonists and that cells expressing other mammalian NMDA receptor do not produce equivalent results.
  • the processes provided herein can be used for the selection of safe NMDA receptor antagonists for the treatment or prevention of a human disorder that lowers the pH in a region of affected tissue, and in particular embodiments is useful for disorders including neuropathic pain, ischemia, Parkinsons disease, epilepsy and traumatic brain injuries.
  • a process is provided to identify a compound that is useful to treat or prevent a disorder that lowers the pH in a region of affected tissue comprising assessing the difference in potency of the compound at physiological pH versus disorder- induced pH (for example, IC 50 at physiologic pH / IC 50 at disorder induced low pH) in a cell that expresses a human NMDA receptor.
  • the assessment of potency boost can include measuring an IC50 of a compound at physiological pH and at disorder-induced pH (the "potency boost") until a 95% confidence interval for the potency boost does not change more than 15% with the addition of a new experiment, wherein the measurements are repeated at least 5 times.
  • the 95% confidence interval does not change more than 10%, more than 8%, more than 5%, more than 4%, more than 3% or more than 2% with the addition of a new experiment.
  • the potency boost experiment is repeated 5 times, at least 6 times, at least 7 times, at least 8 times, at least 9 times, or at least 10 times.
  • a process to identify a compound that is useful to treat or prevent a pain disorder in a region of affected tissue comprising: (i) assessing potency of a compound at inhibiting a human NMDA receptor at physiological pH versus disorder- induced pH in a cell that expresses human NMDA receptors; (ii) testing the compound in vivo and measuring the effect of the compound on a pain threshold; and (iii) selecting a compound that has a potency boost of at least 5 according to step (i) and is associated with at least a 2-fold increase in pain threshold according to step (ii).
  • a process to identify a compound that is useful to treat or prevent a disorder that lowers the pH in a region of affected tissue comprising: (i) assessing the potency boost of the compound at physiological pH versus disorder-induced low pH in a cell that expresses human NMDA receptors by repeating the potency boost experiment at least 5 times and until the 95% confidence interval does not change more than 15% with the addition of a new experiment; (ii) testing the compound in an animal model of neuropathic pain and measuring the effect of the compound on the increase in pain threshold by repeating the experiment at least 5 times and until the 95% confidence interval does not change more than 15% with the addition of a new experiment; (iii) selecting a compound that has a potency boost of at least 5 according to step (i) and is associated with at least a 2-fold increase in pain threshold according to step (ii).
  • the 95% confidence interval obtain in step (i) does not change more than 15%, more than 10%, more than 8%, more than 5%, more than 4%, more than 3% or more than 2% with the addition of a new experiment.
  • the potency boost experiment of step (i) is repeated 5 times, at least 6 times, at least 7 times, at least 8 times, at least 9 times, or at least 10 times.
  • the 95% confidence interval obtain in step (ii) does not change more than 15%, more than 10%, more than 8%, more than 5%, more than 4%, more than 3% or more than 2% with the addition of a new experiment. In a particular subembodiment, the 95% confidence interval obtain in step (ii) does not change more than 5%.
  • the potency boost experiment of step (ii) is repeated 5 times, at least 6 times, at least 7 times, at least 8 times, at least 9 times, at least 10 times, at least 11 times, at least 12 times, at least 13 times, at least 14 times, at least 15 times, at least 16 times, at least 17 times, at least 18 times, at least 19 times, or at least 20 times.
  • the potency boost experiment of step (ii) is repeated at least 12 times.
  • the disorder that lowers the pH in a region of affected tissue is neuropathic pain.
  • a process to identify a compound that is useful to treat or prevent neuropathic pain comprising: (i) assessing the potency boost of the compound at physiological pH versus disorder-induced low pH in a cell that expresses human NMDA receptors by repeating the potency boost experiment at least 5 times and until the 95% confidence interval does not change more than 15% with the addition of a new experiment; (ii) testing the compound in an animal model of neuropathic pain and measuring the effect of the compound on the increase in pain threshold by repeating the experiment at least 12 times and until the 95% confidence interval does not change more than 5% with the addition of a new experiment; (iii) selecting a compound that has a potency boost of at least 5 according to step (i) and is associated with at least a 2-fold increase in pain threshold according to step (ii).
  • the cell can express an NRl subunit and at least one NR subunit of an human NMDA receptor.
  • the NR2 subunit canbe the NR2B subunit.
  • the NR2 subunit can be the NR2A subunit.
  • the physiological pH is about 7.6.
  • the compounds identified by the processes described herein have enhanced activity in brain tissue having lower-than-normal pH due to pathological conditions.
  • the acidic environment generated by ischemic tissue during stroke or by other disorders is harnessed as a switch to activate the neuroprotective agents described herein. In this way side effects are minimized in unaffected tissue since drug at these sites are less active.
  • Conditions that can alter the regional pH include hypoxia resulting from stroke, traumatic brain injury, global ischemia that may occur during cardiac surgery, hypoxia that may occur following cessation of breathing, pre-eclampsia, spinal cord trauma, epilepsy, status epilepticus, neuropathic pain, inflammatory pain, chronic pain, vascular dementia or glioma tumors.
  • the IC50 value of the compound is 0.01 to 10 ⁇ M, 0.01 to 9 ⁇ M, 0.01 to 8 ⁇ M, 0.01 to 7 ⁇ M, 0.01 to 6 ⁇ M, 0.01 to 5 ⁇ M, 0.01 to 4 ⁇ M, 0.01 to 3 ⁇ M, 0.01 to 2 ⁇ M, 0.01 to 1 ⁇ M, 0.05 to 7 ⁇ M, 0.05 to 6 ⁇ M, 0.05 to 5 ⁇ M, 0.05 to 4 ⁇ M, 0.05 to 3 ⁇ M, 0.05 to 2 ⁇ M, 0.05 to 1 ⁇ M, 0.05 to 0.5 ⁇ M, 0.1 to 7 ⁇ M, 0.1 to 6 ⁇ M, 0.1 to 5 ⁇ M, 0.1 to 4 ⁇ M, 0.1 to 3 ⁇ M, 0.1 to 2 ⁇ M, 0.1 to 1 ⁇ M, 0.1 to 0.5 ⁇ M, 0.1 to 7 ⁇ M, 0.1 to 6 ⁇ M, 0.1 to 5 ⁇ M, 0.1 to 4 ⁇ M, 0.1 to
  • the compound exhibits a potency boost of at least 2, at least 3, at least 4, at least 5, at least 6, at least 7, at least 8, at least 9, at least 10, at least 15 or at least 20 when comparing the ICs 0 at physiological pH versus the IC50 diseased pH (i.e., (IC50 at phys pH/IC 50 at Dis pH)).
  • the compound has an IC 50 value of less than 10 ⁇ M at a pH of about 6 to about 9. In one embodiment, the compound has an IC 5 0 value of less than 10 ⁇ M at a pH of about 6.9. In another embodiment, the compound has an IC50 value of less than 10 ⁇ M at a pH of about 7.6. In one embodiment, the compound has an IC5 0 value of less than 10 ⁇ M at physiological pH. In one embodiment, the compound has an IC50 value of less than 10 ⁇ M at diseased pH.
  • the IC50 value of the compound at pH 6.9 is 0.01 to 10 ⁇ M, 0.01 to 9 ⁇ M, 0.01 to 8 ⁇ M, 0.01 to 7 ⁇ M, 0.01 to 6 ⁇ M, 0.01 to 5 ⁇ M, 0.01 to 4 ⁇ M, 0.01 to 3 ⁇ M, 0.01 to 2 ⁇ M, 0.01 to 1 ⁇ M, 0.05 to 7 ⁇ M, 0.05 to 6 ⁇ M, 0.05 to 5 ⁇ M, 0.05 to 4 ⁇ M, 0.05 to 3 ⁇ M, 0.05 to 2 ⁇ M, 0.05 to 1 ⁇ M, 0.05 to 0.5 ⁇ M, 0.1 to 7 ⁇ M, 0.1 to 6 ⁇ M, 0.1 to 5 ⁇ M, 0.1 to 4 ⁇ M, 0.1 to 3 ⁇ M, 0.1 to 2 ⁇ M, 0.1 to 1 ⁇ M, 0.1 to 0.5 ⁇ M, 0.1 to 7 ⁇ M, 0.1 to 6 ⁇ M, 0.1 to 5 ⁇ M, 0.1 to 4 ⁇ M
  • the IC50 value of the compound at pH 6.9 is 0.01 to 10 ⁇ M, 0.01 to 9 ⁇ M, 0.01 to 8 ⁇ M, 0.01 to 7 ⁇ M, 0.01 to 6 ⁇ M, 0.01 to 5 ⁇ M, 0.01 to 4 ⁇ M, 0.01 to 3 ⁇ M, 0.01 to 2 ⁇ M, 0.01 to 1 ⁇ M, 0.05 to 7 ⁇ M, 0.05 to 6 ⁇ M, 0.05 to 5 ⁇ M, 0.05 to 4 ⁇ M, 0.05 to 3 ⁇ M, 0.05 to 2 ⁇ M, 0.05 to 1 ⁇ M, 0.05 to 0.5 ⁇ M, 0.1 to 7 ⁇ M, 0.1 to 6 ⁇ M, 0.1 to 5 ⁇ M, 0.1 to 4 ⁇ M, 0.1 to 3 ⁇ M, 0.1 to 2 ⁇ M, 0.1 to 1 ⁇ M, 0.1 to 0.5 ⁇ M, 0.1 to 7 ⁇ M, 0.1 to 6 ⁇ M, 0.1 to 5 ⁇ M, 0.1 to 4 ⁇ M
  • the IC50 value of the compound at pH about 7.6 is 0.01 to 10 ⁇ M, 0.01 to 9 ⁇ M, 0.01 to 8 ⁇ M, 0.01 to 7 ⁇ M, 0.01 to 6 ⁇ M, 0.01 to 5 ⁇ M, 0.01 to 4 ⁇ M, 0.01 to 3 ⁇ M, 0.01 to 2 ⁇ M, 0.01 to 1 ⁇ M, 0.05 to 7 ⁇ M, 0.05 to 6 ⁇ M, 0.05 to 5 ⁇ M, 0.05 to 4 ⁇ M, 0.05 to 3 ⁇ M, 0.05 to 2 ⁇ M, 0.05 to 1 ⁇ M, 0.05 to 0.5 ⁇ M, 0.1 to 7 ⁇ M, 0.1 to 6 ⁇ M, 0.1 to 5 ⁇ M, 0.1 to 4 ⁇ M, 0.1 to 3 ⁇ M, 0.1 to 2 ⁇ M, 0.1 to 1 ⁇ M, 0.1 to 0.5 ⁇ M, 0.1 to 7 ⁇ M, 0.1 to 6 ⁇ M, 0.1 to 5 ⁇ M, 0.1 to 4 ⁇
  • a method is provided to select a compound that exhibits a potency boost of at least 5 as determined in experiments in which the potency boost of the compound is assessed by comparing the potency at physiological pH versus "disorder- induced low pH" (for example, IC50 at phys pH / IC5 0 at "disorder induced low pH") as tested in a cell expressing a human NMDA receptor by repeating the potency boost experiments at least five times and until the 95% confidence interval does not change more than 15% with the addition of a new experiment.
  • “disorder- induced low pH” for example, IC50 at phys pH / IC5 0 at "disorder induced low pH
  • a method is provided to select a compound or a compound is selected that exhibits at least a 2-fold increase in pain threshold as measured in an animal model of neuropathic pain as determined by repeating the experiment at least 15 times and until the 95% confidence interval does not change more than 15% with the addition of a new experiment.
  • the "disorder- induced low pH" can be associated with an ischemic disorder, such as stroke.
  • the compound selected according to the processes and methods described herein is a selective NR1/NR2A human NMDA receptor and/or a NR1/NR2B human NMDA receptor antaogonist.
  • the compound is not an NMDA receptor channel blocker.
  • the compound does not exhibit substantial toxic side effects, such as, for example, motor impairment, cognitive impairment and cardiac toxicity.
  • the compound has a therapeutic index equal to or greater than at least 2: 1.
  • the compound is at least 10 times more selective for binding to the NMDA receptor than any other glutamate receptor.
  • oocyte cells are used to determine the potency boost.
  • the middle cerebral artery occlusion model is used as the animal model of transient focal ischemia, for example, in rodents, such as mice.
  • the compound exhibits a potency boost of at least 6, 7, 8, 9, 10, 15 or 20 according to step (i) and at least a 2-fold, 3-fold, 4-fold, 5-fold, 6-fold, 7-fold, 8- fold, 9-fold, 10-fold increase in pain threshold according to step (ii).
  • methods to attenuate the progression of an ischemic or excitotoxic cascade associated with a drop in pH by administering a compound selected according to the processes or methods described herein are provided.
  • methods are provided to decrease infarct volume associated with a drop in pH by administering a compound selected according to the processes or methods described herein.
  • a method is provided to decrease cell death associated with a drop in pH by administering a compound selected according to the processes or methods described herein.
  • methods are provided to decrease behavioral deficits associated with an ischemic event associated with a drop in pH by administering a compound selected according to the processes or methods described herein.
  • methods are provided to treat patients with ischemic injury or prevent or treat the neuronal toxicity associated with ischemic injury by administering a compound selected according to the methods or processes described herein.
  • methods are provided to treat the following diseases or neurological conditions, including, but not limited to: Parkinson's disease, chronic nerve injury, chronic pain syndromes, such as, but not limited to diabetic neuropathy, ischemia, ischemia following transient or permanent vessel occlusion, seizures, "spreading depression", hypocapnia, hypercapnia, diabetic ketoacidosis, fetal asphyxia, cognitive deficit after bypass surgery, vasospasm after subarachnoid hemorrhage, spinal cord injury, traumatic brain injury, status epilepticus, epilepsy, hypoxia, perinatal hypoxia, concussion, migraine, hypocapnia, hyperventilation, lactic acidosis, fetal asphyxia during parturition, brain gliomas, and/or retinopathies
  • Parkinson's disease chronic nerve
  • compounds selected according to the methods or processes described herein can be used prophylactically to prevent or protect against such diseases or neurological conditions, for example, in patients with a predisposition for an ischemic event, such as a genetic predisposition, or in patients that exhibit vasospasms, or in patients that have undergone cardiac bypass surgery.
  • a predisposition for an ischemic event such as a genetic predisposition
  • vasospasms or in patients that have undergone cardiac bypass surgery.
  • oocyte describes the mature animal ovum which is the final product of oogenesis and also the precursor forms being the oogonium, the primary oocyte and the secondary oocyte respectively.
  • Transfection refers to the introduction of DNA into a host cell. Cells do not naturally take up DNA. Thus, a variety of technical "tricks" are utilized to facilitate gene transfer. Numerous methods of transfection are known to the ordinarily skilled artisan, for example, CaP ⁇ 4 methods, lipid-based methods and electroporation. (J. Sambrook, E. Fritsch, T. Mat ⁇ atis, Molecular Cloning: A Laboratory Manual, Cold Spring Laboratory Press, 1989).
  • the potency boost of a compound is determined in a cell expressing at least one human NMDA receptor.
  • the cell can endogenously express human NMDA receptors.
  • Cells that can endogenously express NMDA receptors include, but are not limited to: stem cells, Pl 9 cells, neuroepithelial cells, neuroendothelial cells, dopaminergic substantia nigra neurons, astrocytes, magnocellular neuroendocrine cells, supraoptic neurons, cerebellar neurons, brain stem cells, diencephalic neurons, midbrain neurons, hindbrain neurons, spinal cord motor neurons, spinal cord interneurons, dorsal horn neurons, cortical neurons, cerebellar granule cells, hippocampal neurons, septum neurons, caudate cells, putaman cells, striatal cells, olfactory bulb cells, thalamic cells, CAl pyramidal cells, basal ganglia cells, layer IV neurons of rat visual cortex, somatosensory
  • the cell can be genetically modified to express human NMDA receptors.
  • oocyte cells can be genetically modified to express human NMDA receptors.
  • Any suitable oocyte can be used as known by one skilled in the art, including, but not limited to frog oocytes, such as Xenopus oocytes, which include, but are not limited to Xenopus laevis, Xenopus tropicalis, Xenopus muelleri, Xenopus wittei, Xenopus gilli, and Xenopus borealis.
  • the oocytes can be isolated from the ovaries of the animal according to any technique known to one skilled in the art.
  • any suitable cell type can be genetically modified to express human NMDA receptors, including, but not limited to: Chinese hamster ovary (CHO) cells, HEK kidney cells, bacterial cells, E. coli cells, yeast cells, neuronal cells, heart cells, lung cells, stomach cells, spleen cells, pancreas cells, kidney cells, liver cells, intestinal cells, skin cells, hair cells, hypothalamic cells, pituitary cells, epithelial cells, fibroblast cells, neural cells, keratinocytes, hematopoietic cells, melanocytes, chondrocytes, lymphocytes (B and T), macrophages, monocytes, mononuclear cells, cardiac muscle cells, other muscle cells, cumulus cells, epidermal cells, endothelial cells, Islets of Langerhans cells, blood cells, blood precursor cells, bone cells, bone precursor cells, neuronal stem cells, primordial stem cells, hepatocytes, keratin
  • the cell can be genetically modified to express selected human NMDA receptor subunits.
  • NMDA receptors are composed of NRl, NR2 (A, B, C, and D), and NR3 (A and B) subunits, which determine the functional properties of native NMDA receptors.
  • NMDA receptors are heteromeric proteins composed of NRl with NR2 and/or NR3 subunits. DNA encoding any of the NMDA receptor subunits from humans can be used to genetically modify the cells.
  • Table 1 provides the GenEMBL Accession numbers for human NMDA receptor subunits. Table 1
  • the mRNA for example, can be synthesized from the cDNA template and then injected into the cell.
  • the cDNA encoding the receptor subunit can be inserted into a construct or vector prior to insertion into the cell.
  • Techniques which can be used to allow the DNA construct or vector entry into the host cell include calcium phosphate/DNA co-precipitation, microinjection of DNA into the nucleus, electroporation, bacterial protoplast fusion with intact cells, transfection, or any other technique known by one skilled in the art.
  • the DNA can be linear or circular, relaxed or supercoiled DNA. For various techniques for transfecting mammalian cells, see, for example, Keown et al., Methods in Enzymology Vol. 185, pp. 527-537 (1990).
  • the construct or vector can be prepared in accordance with methods known in the art.
  • the construct can be prepared using a bacterial vector, including a prokaryotic replication system, e.g. an origin recognizable by E. coli, at each stage the construct can be cloned and analyzed.
  • a selectable marker can also be employed.
  • the present invention further includes recombinant constructs comprising one or more of the sequences as described above.
  • the constructs can be in the form of a vector, such as a plasmid or viral vector, into which a sequence of the invention can be inserted, in a forward or reverse orientation.
  • the construct can also include regulatory sequences, including, for example, a promoter, operably linked to the sequence. Large numbers of suitable vectors and promoters are known to those of skill in the art, and are commercially available.
  • the following vectors are provided by way of example: pBs, pQE-9 (Qiagen), phagescript, PsiX174, pBluescript SK, pBsKS, pBSSK, pGEM, pNH8a, pNHl ⁇ a, pNH18a, pNH46a (Stratagene); pTrc99A, pKK223-3, pKK233-3, pDR540, pRIT5 (Pharmacia).
  • Eukaryotic pCiNeo, pWLneo, pSv2cat, pOG44, pXTl, pSG (Stratagene) pSVK3, pBPv, pMSG, pSVL (Pharmiacia).
  • any other plasmids and vectors can be used as long as they are replicable and viable in the host.
  • Vectors known in the art and those commercially available (and variants or derivatives thereof) can be used in accordance with the invention be engineered to include one or more recombination sites for use in the methods of the invention.
  • Such vectors can be obtained from, for example, Vector Laboratories Inc., Invitrogen, Promega, Novagen, NEB, Clontech, Boehringer Mannheim, Pharmacia, EpiCenter, OriGenes Technologies Inc., Stratagene, PerkinElmer, Pharmingen, and Research Genetics.
  • vectors of interest include eukaryotic expression vectors such as pFastBac, pFastBacHT, pFastBacDUAL, pSFV, and pTet-Splice (Invitrogen), pEUK-Cl, pPUR, pMAM, pMAMneo, pBHOl, pBI121, pDR2, pCMVEBNA, and pYACneo (Clontech), pSVK3, pSVL, pMSG, pCHl lO, and pKK232-8 (Pharmacia, Inc.), p3'SS, pXTl, ⁇ SG5, pPbac, pMbac, pMClneo, and pOG44 (Stratagene, Inc.), and pYES2, pAC360, pBlueBacHis A, B, and C, pVL1392, pBlueBa
  • Additional vectors suitable for use in the invention include pUC18, pUC19, pBlueScript, pSPORT, cosmids, phagemids, YACs (yeast artificial chromosomes), BACs (bacterial artificial chromosomes), Pl (Escherichia coli phage), pQE70, pQE60, pQE9 (quagan), pBS vectors, PhageScript vectors, BlueScript vectors, pNH8A, pNH16A, pNH18A, pNH46A (Stratagene), pcDNA3 (Invitrogen), pGEX, pTrsfus, pTrc99A, pET-5, pET-9, pKK223-3, pKK233-3, pDR540, pRIT5 (Pharmacia), pSPORTl, pSPORT2, pCMVSPORT2.0 and pSV-SPORTl (Invitrogen) and variants or derivatives
  • Viral vectors can also be used, such as lentiviral vectors (see, for example, WO 03/059923; Tiscornia et al. PNAS 100:1844-1848 (2003)). Additional vectors of interest include pTrxFus, pThioHis, pLEX, pTrcHis, pTrcHis2, pRSET, pBlueBacHis2, pcDNA3.
  • Additional vectors include, for example, pPC86, pDBLeu, pDBTrp, pPC97, p2.5, pGADl-3, pGADIO, pACt, pACT2, pGADGL, pGADGH, pAS2-l, pGAD424, pGBT8, pGBT9, pGAD-GAL4, pLexA, pBD-GAL4, pHlSi, pHISi-1, placZi, pB42AD, pDG202, pJK202, pJG4-5, pNLexA, pYESTrp and variants or derivatives thereof.
  • Selectable markers can also be inserted into the vector to allow for selection of cells that contain the human NMDA receptor subunit.
  • Suitable selectable marker include, but are not limited to: genes conferring the ability to grow on certain media substrates, such as the tk gene (thymidine kinase) or the hprt gene (hypoxanthine phosphoribosyltransferase) which confer the ability to grow on HAT medium (hypoxanthine, aminopterin and thymidine); the bacterial gpt gene (guanine/xanthine phosphoribosyltransferase) which allows growth on MAX medium (mycophenolic acid, adenine, and xanthine).
  • selectable markers include: genes conferring resistance to compounds such as antibiotics, genes conferring the ability to grow on selected substrates, genes encoding proteins that produce detectable signals such as luminescence or fluorescence, such as green fluorescent protein, enhanced green fluorescent protein (eGFP).
  • markers are known and available, including, for example, antibiotic resistance genes such as the neomycin resistance gene (neo) (Southern, P., and P. Berg, J. MoI. Appl. Genet. 1 :327-341 (1982)); and the hygromycin resistance gene (hyg) (Nucleic Acids Research U -.6895-69 U (1983), and Te Riele, H., et al, Nature 348:649-651 (1990)).
  • antibiotic resistance genes such as the neomycin resistance gene (neo) (Southern, P., and P. Berg, J. MoI. Appl. Genet. 1 :327-341 (1982)
  • hygromycin resistance gene hygromycin resistance gene (hyg) (Nucleic Acids Research U -.6895-69 U (1983), and Te Riele, H., et al, Nature 348:649-651 (1990)).
  • selectable marker genes include: acetohydroxy acid synthase (AHAS), alkaline phosphatase (AP), beta galactosidase (LacZ), beta glucoronidase (GUS), chloramphenicol acetyltransferase (CAT), green fluorescent protein (GFP), red fluorescent protein (RFP), yellow fluorescent protein (YFP), cyan fluorescent protein (CFP), horseradish peroxidase (HRP), luciferase (Luc), nopaline synthase, octopine synthase (OCS), and derivatives thereof.
  • AHAS acetohydroxy acid synthase
  • AP alkaline phosphatase
  • LacZ beta galactosidase
  • GUS beta glucoronidase
  • CAT chloramphenicol acetyltransferase
  • GFP green fluorescent protein
  • RFP red fluorescent protein
  • YFP yellow fluorescent protein
  • CFP cyan fluorescent protein
  • HRP horseradish
  • Multiple selectable markers are available that confer resistance to ampicillin, bleomycin, chloramphenicol, gentamycin, hygromycin, kanamycin, lincomycin, methotrexate, phosphinothricin, puromycin, and tetracycline.
  • Cells that have been successfully transformed to express the human NMDA receptor can be confirmed via function analysis or molecular analysis.
  • cells such as oocytes, in which human NMDA receptor subunit cRNA has been inserted can be tested via electrophysiological recordings for the presence of functional human NMDA receptors.
  • cells, in which the DNA encoding the human NMDA receptor subunit gene(s) and a selectable marker gene has been inserted can then be grown in appropriately-selected medium to identify cells providing the appropriate integration. Those cells which show the desired phenotype can then be further analyzed by restriction analysis, electrophoresis, Southern analysis, polymerase chain reaction, or another technique known in the art. By identifying fragments which show the appropriate insertion at the target gene site, cells can be identified in which homologous recombination has occurred to inactivate or otherwise modify the target gene.
  • cells expressing human NMDA receptors can then be used to determine the potency boost of a particular compound, such as the compounds described according to the methods and processes herein.
  • the potency boost of the compound can be determined by testing the effects of the compound at physiological pH versus disorder-induced low pH in a cell expressing a human NMDA receptor by repeating the potency boost experiment until the 95% confidence interval does not change more than 15% with the addition of a new experiment.
  • a method is provided to select a compound or a compound is selected that exhibits a potency boost of at least 5 as determined in experiments in which the potency boost of the compound is assessed at physiological pH versus disorder-induced low pH as tested in a cell expressing a human NMDA receptor by repeating the potency boost experiments at least five times and until the 95% confidence interval does not change more than 15% with the addition of a new experiment.
  • Disorder-induced low pH is defined as a drop in pH associated with any of the disorders or diseases referred to herein.
  • the "disorder-induced low pH” can be between about 6.4 and about 7.1, generally about 6.4, 6.5, 6.6, 6.7, 6.8, 6.9, 7.0, or 7.1.
  • Physiological brain-tissue pH is between about 7.2 and about 7.8, generally about 7.2, 7.3, 7.4, 7.5, 7.6, 7.7, or 7.8.
  • the "disorder-induced low pH” can be associated with an ischemic disorder, such as stroke.
  • disorder-induced low pH is about 6.9. In another embodiment, disorder-induced low pH is from about 6.7 to about 7.1.
  • physiological pH is about 7.6. In another embodiment, physiological pH is from about 7.4 to about 7.8.
  • IC 50 values concentration of the compound that causes a half-maximal inhibition of NMDA channel function (IC 50 values) for a compound at physiological pH, such as pH 7.6, and ischemic or neuropathic pain pH, such as pH 6.9. Any method known in the art to determine IC 50 values for a compound can be used. The IC 50 values can be expressed as a ratio and averaged together to determine the mean shift in IC 50 . In one embodiment, two electrode voltage-clamp recordings can be used to determine ICso values for a compound. Glass microelectrodes can be filled with potassium chloride, such that the voltage electrode contains a lower concentration of potassium chloride than the current electrode. The cells can be placed in a chamber and perfused with physiological solution.
  • External pH can be adjusted to either ischemic or neuropathic pain pH, such as pH 6.9 or physiological pH, such as pH 7.6.
  • Dose response curves can then be obtained by applying in successive fashion maximally effective concentrations of glutamate and glycine, followed by glutamate/glycine plus variable concentrations of test compound.
  • the level of inhibition by applied antagonist can be expressed as a percent of the initial glutamate response.
  • the average percent responses at each of the antagonist concentrations can be fitted by the logistic equation, (100-min)/(l+([conc]/IC50) nH ) + min, where min is the residual percent response in saturating antagonist, IC 50 is the concentration of antagonist that causes half of the achievable inhibition, and nH is a slope factor describing steepness of the inhibitory curve.
  • Min can be constrained to be greater than or equal to 0.
  • min can be set to 0.
  • the IC 5 0 values obtained at physiological pH and ischemic pH can then be expressed as a ratio and averaged together to determine the mean shift in IC 5 0.
  • the compound can exhibit a potency boost of at least 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22 or greater than 23 at physiological pH versus disorder-induced low pH.
  • the potency boost experiments can be repeated until the 95% confidence interval does not change more than 15% with the addition of a new experiment. In another embodiment, the potency boost experiments can be repeated until the 95% confidence interval does not change more than about 14%, 13,%, 12%, 11%, 10%, 9%, 8%, 7%, 6%, 5%, 4%, 3% or 2% with the addition of a new experiment. In a further embodiment, the potency boost experiments can be repeated until the 96%, 97%, 98% or 99% confidence interval does not change more than about 10%, 9%, 8%, 7%, 6%, 5%, 4%, 3% or 2% with the addition of a new experiment.
  • a process is provided to identify a chemical compound that is useful to treat or prevent a disorder that lowers the pH in a region of affected tissue comprising: (i) assessing the potency boost of the compound at physiological pH versus disorder-induced low pH in a cell expressing a human NMDA receptor by repeating the potency boost experiment until the 95% confidence interval does not change more than 15% with the addition of a new experiment; (ii) testing the compound in an animal model of neuropathic pain and measuring the effect of the compound on the increase in pain threshold by repeating the experiment until the 95% confidence interval does not change more than 5% with the addition of a new experiment; (iii) selecting a compound that has a potency boost of at least 5 according to step (i) and is associated with at least a 2-fold increase in pain threshold according to step (ii).
  • a candidate drug must meet or exceed both the in vitro and in vivo criteria to be a superior drug for human use.
  • a method is provided to select a compound or a compound is selected that exhibits at least a 2-fold increase in pain threshold as measured in an animal model of neuropathic pain as determined by repeating the experiment at least 15 times and until the 95% confidence interval does not change more than 15% with the addition of a new experiment.
  • the compounds disclosed herein can be useful for the treatment or prevention of pain, in particular neuropathic pain and related disorders.
  • a process is provided to identify a chemical compound that is useful to treat neuropathic pain in a mammal, particularly a human, by: (i) assessing the potency boost of the compound at physiological pH versus "disorder-induced pH" (for example, IC 5O at physiological pH/ICso at “disorder induced low pH") in a cell by repeating the potency boost experiment at least 5 times such that the 95% confidence interval does not change more than 15% with the addition of a new experiment; (ii) testing the compound in an animal model of neuropathic pain and measuring the effect of the compound on the increase in pain threshold by repeating the experiment at least 12 times such that the 95% confidence interval does not change more than 5% with the addition of a new experiment; (iii) selecting a compound that has a potency boost of at least 5 according to step (i) and at least a 2-fold increase in pain threshold according to step (ii).
  • the potency boost can be determined is a cell that expressed a glutamate receptor derived from a human. In another embodiment, the potency boost can be determined in a cell that expresses at least one human- derived NMDA, AMPA, and/or kainate receptor. In one embodiment, the cell can express an NRl subunit and at least one NR2 subunit of an NMDA receptor. In a further embodiment, the NR2 subunit can be the NR2B subunit. In another embodiment, the NR2 subunit can be the NR2A subunit.
  • a process wherein a compound to treat a disorder that lowers the pH in a manner that activates an NMDA receptor antagonist is selected that (i) exhibits a potency boost of at least 5 as determined in experiments in which the potency boost of the compound at assessing the potency boost of the compound at physiological pH versus "disorder-induced low pH" (for example, IC 5 0 at phys PH/IC 50 at "disorder induced low pH") is tested in a cell by repeating the potency boost experiments at least 5 times such that the 95% confidence interval does not change more than 15% with the addition of a new experiment and (ii) testing the compound in an animal model of neuropathic pain and measuring the effect of the compound on the increase in pain threshold by repeating the experiment at least 12 times such that the 95% confidence interval does not change more than 5% with the addition of a new experiment; (iii) selecting a compound that has a potency boost of at least 5 according to step (i) and at
  • the potency boost can be determined is a cell that expressed a glutamate receptor. In another embodiment, the potency boost can be determined in a cell that expresses an NMDA, AMPA, and/or kainate receptor. In one embodiment, the cell can express an NRl subunit and at least one NR2 subunit of an NMDA receptor. In a further embodiment, the NR2 subunit can be the NR2B subunit. In another embodiment, the NR2 subunit can be the NR2A subunit.
  • the animal model of neuropathic pain can be selected from the group including, but not limited to: a chronic constriction injury model, a partial sciatic ligation model, a spinal nerve ligation model or any other model known to one skilled in the art.
  • the spinal nerve ligation model is used as the in vivo animal model.
  • the "pain threshold” is a measure of the amount of stimulation required before the sensation of pain is experienced.
  • chronic neuropathic pain animal models animals are subject to injury and a state of chronic pain induced. Noxious stimuli can then be applied and the amount of time that the animal can tolerate the noxious stimuli without reacting to it can be calculated. For example, an uninjured animal could be exposed to a cold surface for 20 minutes before withdrawing its paw from the surface, but after an injury, such as one described below to model neuropathic pain, the animal may withdraw its paw after only 1 minute.
  • noxious stimuli include, but are not limited to: heat, cold, mechanical, such as von Frey's stimulus, chemical and the like.
  • the chronic constriction injury model (CCI, or the Bennett model) can be used as the animal model of neuropathic pain (see, for example, Bennett, Gary J. et al. Pain, 1988, 33, 87-107).
  • the sciatic nerve of an animal for example, a rat
  • the sciatic nerve can be intentionally injured in a manner that was discovered to induce symptoms reported by human patients with neuropathic pain.
  • the sciatic nerve can be exposed at midthigh, proximal to the nerve's trifurcation in the popliteal fossa. At that location, about 7 mm of the nerve's trajectory can be freed of adhering tissue and four ligatures tied loosely around it, with about 1-mm spacing.
  • each animal an identical dissection can be performed contralateral ⁇ without ligation so that each animal can serve as its own control.
  • the affected hindpaw skin becomes unequivocally hyperalgesic and allodynic (i.e., experiences pain resulting from a stimulus that ordinarily does not elicit a painful response), and perhaps a source of spontaneous pain as well.
  • a noxious stimuli such as heat, can be aimed at the plantar hitidpaw from beneath a glass floor and the latency for paw withdrawal (a marker for pain threshold) can be measured.
  • the responses on the nerve-injured side tend to be of abnormal magnitude and duration, exceeding, for example, 30 seconds of paw elevation, and can be accompanied by prolonged licking.
  • a normal response would be that the animal barely raise the paw and would last less than a second or two.
  • the animals can be placed on a metal floor cooled, for example, at a temperature of 4°C.
  • the floor produces no pain, even after 20 minutes of contact.
  • Rats with ligation can be measured for withdrawals of the nerve-injured paw, which, for example, can increase more than fivefold, and the duration can be measured, it can increase, for example, more than twofold.
  • pain threshold can be calculated without drug and also after administration of a compound described herein.
  • the partial sciatic ligation model (the Seltzer model) can be used to test neuropathic pain threshold (see, Seltzer, A. et al. Pain, 1990, 43, 205-218).
  • the Seltzer model half of the sciatic nerve high in the thigh of an animal, such as a rat, can be unilaterally ligated.
  • the animals can develop guarding behavior of the ipsilateral hind paw and lick it often, suggesting the possibility of spontaneous pain.
  • the plantar surface of the foot can be evenly hyperesthetic to non-noxious and noxious stimuli. Common measurements to noxious stimuli can be measured in the animal with and without exposure to the compounds of the present invention.
  • Noxious stimuli can include the Von Frey hair stimulation, CO 2 laser heat pulses and pin procks.
  • Von Frey hair stimulation In response to repetitive Von Frey hair stimulation at the plantar side, there can be a sharp decrease in the withdrawal thresholds. After a series of such stimuli in the operated side, light touch elicits aversive responses, suggesting allodynia to touch.
  • the withdrawal thresholds to CO 2 laser heat pulses is also markedly lowered.
  • Suprathreshold noxious heat pulses elicit exaggerated responses unilaterally, suggesting thermal hyperalgesia.
  • Pin-pricks also can evoke such exaggerated responses (mechanical hyperalgesia).
  • pain threshold can be calculated without drug and also after administration of a compound described herein.
  • the spinal neive ligation model (the Chung model) can be used to measure neuropathic pain (see Kim, S.H. and Chung, J.M. Neurosci. Lett. 1991, 134, 131-134; Kim, S.H. and Chung, J.M. Pain, 1992, 50, 355-363).
  • the L 5 (or L 5 + L ⁇ ) spinal nerves are tightly ligated and then cut. The surgical procedure produces a long- lasting hyperalgesia to noxious heat and mechanical allodynia of the affected foot. Mechanical sensitivity of the affected hind paw can be measured.
  • compounds can be selected that result in at least a 2-fold increase in pain threshold.
  • the compound can exhibit at least a 3, 4, 5, 6, 7, 8, 9, 10, 15, 20 or 30 fold increase in pain threshold.
  • the experiment can be repeated at least 15 times and until the 95% confidence interval does not change more than 10% with the addition of a new experiment.
  • the neuropathic pain experiments can be repeated until the 95% confidence interval does not change more than 10% with the addition of a new experiment.
  • the neuropathic pain experiments can be repeated until the 95% confidence interval does not change more than about 9%, 8%, 7%, 6%, 5%, 4%, 3% or 2% with the addition of a new experiment. In a further embodiment, the neuropathic pain experiments can be repeated until the 96%, 97%, 98% or 99% confidence interval does not change more than about 10%, 9%, 8%, 7%, 6%, 5%, 4%, 3% or 2% with the addition of a new experiment.
  • neuropathic pain include, but are not limited to, the spared nerve injury model (see Decosterd & Woolf. Pain. 2000 Aug;87(2): 149-58), sciatic inflammatory neuropathy (SIN) induced by localized inflammation of the sciatic nerve in the absence of frank trauma, and/ or a peripheral nerve model of pain following the injection of the chemotherapeutic agent vincristine (Aley et al Neurosci 1996;73:259-65). Additional models are known to one skilled in the art. See also Zimmerman M. Eur J Pharmacol 2001;429:23-37; Shir et al Neurosci Lett 1990;l 15:62-7.
  • a process is provided to identify a chemical compound that is useful to treat ischemic injury in a human, by: (i) assessing the potency boost of the compound at physiological pH versus "disorder-induced low pH" (for example, IC 50 at physiological pH / IC 50 at "disorder induced low pH") in a cell that expresses human NMDA receptors by repeating the potency boost experiment at least 5 times such that the 95% confidence interval does not change more than 15% with the addition of a new experiment; (ii) testing the compound in an animal model of transient focal ischemia and measuring the effect of the compound on the infarct volume by repeating the experiment at least 12 times such that the 95% confidence interval does not change more than 5% with the addition of a new experiment; (iii) selecting a compound that has a potency boost of at least 5 according to step (i) and at least a 30% decrease in infarct volume according to step (ii).
  • a candidate drug must meet or exceed both the in vitro and in vivo criteria to be a superior drug for human use.
  • the cell can express an NRl subunit and at least one NR2 subunit of an NMDA receptor.
  • the NR2 subunit can be the NR2B subunit.
  • the NR2 subunit can be the NR2A subunit.
  • a process wherein a compound to treat a disorder that lowers the pH in a manner that activates an human NMDA receptor antagonist is selected that (i) exhibits a potency boost of at least 5 as determined in experiments in which the potency boost of the compound is assessed at physiological pH versus "disorder-induced low pH" is tested in a cell by repeating the potency boost experiments at least 5 times such that the 95% confidence interval does not change more than 15% with the addition of a new experiment and (ii) exhibits at least a 30% decrease in infarct volume as measured in an animal model of focal ischemia as determined by repeating the experiment at least 12 times such that the 95% confidence interval does not change more than 5% with the addition of a new experiment.
  • the cell can express an NRl subunit and at least one NR2 subunit of an NMDA receptor.
  • the NR2 subunit can be the NR2B subunit.
  • the NR2 subunit can be the NR2A subunit.
  • a method is provided to select a compound or a compound is selected that exhibits at least a 30% decrease in infarct volume as measured in an animal model of focal ischemia as determined by repeating the experiment at least 15 times and until the 95% confidence interval does not change more than 10% with the addition of a new experiment.
  • the "disorder-induced low pH" can be associated with an ischemic disorder, such as stroke.
  • the middle cerebral artery occlusion model can be used as the animal model of transient focal ischemia, for example, in rodents, such as mice.
  • Focal ischemic stroke can be damage to the brain caused by interruption of the blood supply to a region thereof.
  • the focal ischemic stroke is generally caused by obstruction of any one or more of the "main cerebral arteries" (e.g. middle cerebral artery, anterior cerebral artery, posterior cerebral artery, internal carotid artery, vertebral artery or basilar artery), as opposed to secondary arteries or arterioles.
  • the arterial obstruction can be a single embolus or thrombus.
  • focal ischemic stroke as defined herein is distinguished from the cerebral embolism stroke model (such as described in Bowes et al., Neurology 45:815-819 (1995)) in which a plurality of clot particles occlude secondary arteries or arterioles.
  • Focal ischemia can be induced in any mammal, including, but not limited to, rodents, mice, rats, rabbits and gerbils (see also Renolleau S, Stroke. 1998 Jul;29(7): 1454-60; Gotti, B. et al., Brain Res, 1990, 522, 290-307).
  • the gerbil has been widely used as an experimental model for studies of ischemic stroke because the brain blood supply is controlled by only two common carotid arteries. This unusual feature occurs in gerbils because they have an incomplete circle of Willis (Chandler et al., J. Pharmacol. Methods 14: 137-146, 1985; Finkelstein et al., Restor. Neurol. Neurosci. 1:387-394, 1990; Levine and Sohn, Arch. Pathol. 87:315-317, 1969; Kahn, Neurology 22:510-515, 1972).
  • Test compounds can be administered to the animal prior to or after the occlusion of the artery.
  • the test compound can be administered intraperitoneally.
  • the test compound can be administered intracerebroventricularly.
  • the test compound can be administered prior to the occlusion of the artery, for example, about 10, 20, 30, 40, 50 or 60 minutes prior to the ischemic event.
  • test compound can be administered after the occlusion of the artery, for example, about 10, 20, 30, 40, 50, 60, 90, or 120 minutes or about 4, 6, 8 or 10 hours or about 1, 2, 3, 4, 5, 6, 7 or 8 days after the ischemic event, i.e. post-reperfusion.
  • the demonstration that compounds can protect cells in an ischemic area can be tested in animal models in which the middle cerebral artery (MCA) is experimentally occluded, namely the middle cerebral artery occlusion (MCAO) model.
  • MCA middle cerebral artery
  • MCAO middle cerebral artery occlusion
  • This animal model is well known in the art to simulate an in vivo ischemic event such as may occur in a human subject.
  • the experimental occlusion of the MCA causes a large unilateral ischemic area that typically involves the basal ganglion and frontal, parietal, and temporal cortical areas (Menzies et al. Neurosurgery 31, 100-106 (1992)).
  • the ischemic lesion begins with a smaller core at the site perfused by the MCA and grows with time.
  • This penumbral area around the core infarct is believed to result from a propagation of the lesion from the core outward to tissue that remains perfused by collateral circulation during the occlusion.
  • the effect of a therapeutic agent on the penumbra surrounding the core of the ischemic event may be examined when brain slices are obtained from the animal.
  • the MCA supplies blood to the cortical surfaces of frontal, parietal, and temporal lobes as well as basal ganglia and internal capsule. Slices of the brain can be taken around the region where the greatest ischemic effect occurs.
  • the MCAO can be induced in any mammal, including, but not limited to, mice, rats, rabbits and gerbils, (see also Renolleau S, Stroke.
  • the MCA model allows for an indirect measure of neuronal cell death following an ischemic event (i.e., occlusion of the left middle cerebral artery).
  • ischemic event i.e., occlusion of the left middle cerebral artery.
  • a transient focal cerebral ischemia of the middle cerebral artery can be used to test the compounds.
  • Transient focal cerebral ischemia can be induced by intraluminal middle cerebral artery (MCA) occlusion.
  • Occlusion can be achieved through any means that blocks the artery, for example, with a suture, such as a monofilament suture.
  • a probe can be affixed to their skull to monitor relative changes in regional cerebral blood flow. Such changes can be monitored with a laser Doppler flowmeter (Perimed). For example, in mice, the probe can be affixed 2 mm posterior and 4-6 mm lateral of the bregma. Then, an incision can be made to access the MCA and a material can be inserted to occlude the MCA.
  • a suture can be introduced into the internal carotid artery through the external carotid artery stump until monitored blood flow is stopped. After a period of time of MCA occlusion, such as about 30 minutes, 45 minutes or 60 minutes, blood flow can be restored by withdrawing the blocking material.
  • a bilateral carotid occlusion model can be used to demonstrate that compounds can protect cells in an ischemic area. Animals can be anesthetized and an incision can be made in the ventral neck and the common carotid arteries can be isolated and occluded completely for a period of time, for example 5, 10, 15, 20, 30, 45 or 60 minutes.
  • the artery can be occluded by any means, for example, using a clip, such as a microaneurysm clips.
  • the occlusion can then be stopped and the incision can be sutured.
  • the bilateral carotid occlusion can be conducted in a gerbil.
  • the animals can then be allowed to recover. After the animal survives for a period of time, for example, about 12, 24, 36, 48 or 72 hours, the animal can be sacrificed and the brain removed and sectioned, for example in approximately, 1, 2, 3, 4, 5 or 10 mm sections.
  • the volume of infarct can then be identified by staining the brain sections with an appropriate dye, for example 2% 2,3,5-triphenyltetrazolium chloride (TTC) in PBS at 37°C for approximately 20 minutes.
  • TTC 2,3,5-triphenyltetrazolium chloride
  • the infarct area of each section can then be measured and multiplied by the section thickness to give the infarct volume of that section.
  • a ratio of the contralateral to ipsilateral hemisphere section volume can also be multiplied by the corresponding infarct section volume to correct for edema.
  • Infarct volume can be determined by summing the infarct area times section thickness for all sections.
  • compounds can be selected that result in at least a 30% decrease in infarct volume.
  • compounds can be selected that result in at least a 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 65, 70, 75, 80, 85, 90, 95, 97, 99 or 100% decrease in infarct volume.
  • the compound can exhibit a potency boost of at least 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 30, 40 or 50 at physiological pH versus ischemic pH (i.e., phys pH/Isc pH) and at least a 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 65, 70, 75, 80, 85, 90, 95, 97, 99 or 100% decrease in infarct volume, such as illustrated in Figure 1 , including independently, any combination of these numbers, each combination of which is deemed to be specifically disclosed.
  • ischemic pH i.e., phys pH/Isc pH
  • the mean i.e. the sum of all the observations divided by the number of observations, can be calculated for the potency boost and infarct volume experiments and the mean value of the compound can exhibit a potency boost of at least 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22 or 23 at physiological pH versus ischemic pH (i.e., phys pH/Isc pH) and at least a 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 65, 70, 75, 80 or 80% decrease in infarct volume, such as illustrated in Figure 1.
  • ischemic pH i.e., phys pH/Isc pH
  • the infarct volume experiments can be repeated until the 95% confidence interval does not change more than 10% with the addition of a new experiment. In another embodiment, the infarct volume experiments can be repeated until the 95% confidence interval does not change more than about 25%, 24%, 23%, 22%, 21%, 20%, 19%, 18%, 17%, 16%, 15%, 14%, 13%, 12%, 11%, 10%, 9%, 8%, 7%, 6%, 5%, 4%, 3% or 2% with the addition of a new experiment.
  • the infarct volume experiments can be repeated until the 96%, 97%, 98% or 99% confidence interval does not change more than about 25%, 24%, 23%, 22%, 21%, 20%, 19%, 18%, 17%, 16%, 15%, 14%, 13%, 12%, 11%, 10%, 9%, 8%, 7%, 6%, 5%, 4%, 3% or 2% with the addition of a new experiment.
  • transient focal ischemia include, but are not limited to intraarterial injection of microspheres or coagulated blood, four vessel occlusion in rat, two vessel occlusion in gerbil, or photochemicaly induced clot formation with dissolution. Such models are known to one skilled in the art.
  • the compounds identified by the processes provided herein can be at least 10 times more selective for binding to the human NMDA receptor than any other glutamate receptor other receptor as described herein.
  • the compound can have a therapeutic index equal to or greater than at least 2:1.
  • the compound can be at least 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 78, 85, 90, 95, 100, 125, 150, 175, 200, 300, 400, 500, or 1000 times more selective for binding to the human NMDA receptor than any other glutamate receptor, for example, including, but not limited to the following glutamate receptors: AMPA GIuRl (GenEMBL Accession Nos. X57497, Xl 7184, 157354), AMPA GluR2 (GenEMBL Accession Nos. X57498, M85035, A46056), AMPA GluR3 (GenEMBL Accession Nos.
  • AMPA GluR4 (GenEMBL Accession Nos. M36421, U16129), Kainate GluR5 (GenEMBL Accession Nos. X66118, M83560, U16125), Kainate GluR6 (GenEMBL Accession Nos. D10054, Zl 1715, U16126), Kainate GluR7 (GenEMBL Accession Nos. M83552, U16127), Kainate KA-I (GenEMBL Accession Nos. X59996, S67803 ⁇ ), Kainate KA-2 (GenEMBL Accession Nos. DlOOl 1, Zl 1581, S40369), Orphan dl GRIDl (GenEMBL Accession Nos.
  • NMDA receptor can be made up of any of its subunits, including, but not limited to NMDA NRl (Chromosome (human) 9q34.3, GenEMBL Accession No. for Mouse: D10028, GenEMBL Accession No.
  • NMDA NR2A Chromosome (human): 16pl3.2, GenEMBL Accession No. for Mouse: D 10217, GenEMBL Accession No. for Rat: D13211, GenEMBL Accession No. for Human: U09002)
  • NMDA NR2B Chromosome (human): 12pl2 GenEMBL Accession No. for Mouse: D10651 ' GenEMBL Accession No. for Rat: M91562, GenEMBL Accession No. for Human: U28861 ⁇
  • NMDA NR2C Chromosome (human) 17q24-q25, GenEMBL Accession No.
  • NMDA NR2D Chosome (human) 19ql3.1qter, GenEMBL Accession No. for Mouse: D12822, GenEMBL Accession No. for Rat: D13214, GenEMBL Accession No. for Human: U77783
  • NMDA NR3A GenEMBL Accession No. for Rat: L34938 and/or NMDA NR3B.
  • the compound is not more selective or at least 2, 3, 4, 5, 6, 7, 8, or 9 times more selective for the human NMDA receptor then another glutamate receptor listed above.
  • the compound can be at least 10 times more selective for binding to the NMDA receptor than another receptor type.
  • the compound can be at least 1 1, 12, 13, 14, 15, 16, 17, 18, 19, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 78, 85, 90, 95, 100, 125, 150, 175, 200, 300, 400, 500, or 1000 times more selective for binding to the NMDA receptor than another receptor type, for example, including, but not limited to the following receptors: dopamine receptors, such as Dl, D2, D3, D4 and D5 dopamine receptors; opioid receptors, such as mu opioid receptors, including mul and mu2; delta opioid receptors, including deltal and delta2, and kappa opioid receptors, including kappal and kappa 2; cholinergic receptors, including muscarinic and nicotinic receptors; adrenergic receptors, including epinephrine receptors and epinephrine receptors
  • GHRH Growth hormone releasing hormone
  • LHRH Luteinizing hormone releasing hormone
  • Thyrotropin releasing hormone TRH
  • Neuropeptide tyrosine (NPY)
  • PYY Peptide tyrosine-tyrosine
  • Glucogen-like peptide- 1 (GLP-I)
  • Peptide histidine isoleucine Peptide histidine isoleucine
  • VIP Vasoactive intestinal polypeptide
  • IAPP Islet amyloid polypeptide
  • amylin amylin
  • MCH Melanin concentrating hormone
  • the compound can be at least 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 78, 85, 90, 95, 100, 125, 150, 175, 200, 300, 400, 500, or 1000 times more selective for binding to a human NMDA receptor than a serotonin receptor.
  • the compound is not more selective or at least 2, 3, 4, 5, 6, 7, 8, or 9 times more selective for a human NMDA receptor then a serotonin receptor.
  • Seratonin receptors include, but are not limited to 5HTi, including SHT IA , 5HT IB , 5HTID, 5HT IE , and 5HTi F ; 5HT 2 , including 5HT 2A , 5HT 2B , and 5HT 2C ; 5HT 3 ; 5HT 4 ; 5HT 5 , including 5HT 5a and 5HT 5B ; 5HT ⁇ and 5HT 7 .
  • the compound can be at least 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 78, 85, 90, 95, 100, 125, 150, 175, 200, 300, 400, 500, or 1000 times more selective for binding to a human NMDA receptor than a histamine receptor, including Hl, H2, H3 and H4 histamine receptors.
  • the compound is not more selective or at least 2, 3, 4, 5, 6, 7, 8, or 9 times more selective for a human NMDA receptor then a histamine receptor, including Hl, H2, H3 and H4 histamine receptors.
  • the compound can be at least 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 78, 85, 90, 95, 100, 125, 150, 175, 200, 300, 400, 500, or 1000 times more selective for binding to a human NMDA receptor than a calcium channel.
  • Screening compounds to determine the affinity of a drug for a particular receptor is one of the critical in the drug discovery process. Processes to determine receptor selectivity can be done by any method known to one skilled in the art. Screening can be used as a primary screening method for large compound libraries or as a secondary screen to rank compounds for binding affinity for various receptor types or subtypes. In one embodiment, this analysis can be done in a high throughput system, for example, filter-plate screening systems, such as the Millipore MultiscreenTMH ⁇ s filter plate.
  • radioligand binding assays can be used to determine the receptor selectivity for a particular receptor.
  • saturation binding assays can be used to determine the binding constant (Ka) of a test compound for a particular receptor.
  • Saturation binding assays can be performed according to any method known in the art. In general, saturation binding assays can be conducted by obtaining a cell membrane that expresses a particular receptor. For example, a cell, such as a CHO cell, can be transfected to express aa human NMDA receptor, for example, an NR1/NR2A or NR1/NR2B human NMDA receptor.
  • cells can be used that endogenously expresses a human NMDA receptor, for example, an NR1/NR2B or NR1/NR2A human NMDA receptor.
  • whole cell binding assays can be conducted.
  • membranes can be isolated from the cell, such as, for example, by lysing the cell and then using centrifugation to obtain the membrane fraction of the lysate, see, for example, Laboratory method for isolation of cell membranes, A. Hubbard and Z. Conn The Journal of Cell Biology (1975) and Rogers et al., 1991, J.Neuroscience: 2713-2724.
  • the whole cell or cell membranes can then be incubated with serial dilutions of radiolabeled ligand, i.e. test compound, for example 3H- labeled ligand. After incubation for a period of time, for example, at least 1, 2 or 3 hours, the membranes can be washed a number of times, for example, 5, 10, 15 or 20 times. Scintillation fluid can then be added and the cells or radioactivity of the cells or membranes can be conducted. Non-specific binding can also be determined in a separate experiment with an excess of unlabeled competitor ligand. Specific binding can be calculated as nonspecific activity subtracted from total activity.
  • radiolabeled ligand i.e. test compound, for example 3H- labeled ligand.
  • Binding constants can then be determined by fitting specific binding by free ligand concentration by non-linear regression and Scatchard analysis, for example by using Prizm data software (www.Graphpad.com).
  • the number of binding sites [maximal binding capacities (B max ) can also be calculated by non-linear regression and Scatchard analysis, for example by using Prizm data software.
  • displacement radioligand binging assays can be conducted to determine relative affinity values (IC 50 ).
  • Whole cells expressing particular receptors or isolated cell membranes can be used, as described above. Inhibition can be determined by using a constant radioligand concentration and serial dilutions of unlabelled competitor ligand as compared to a control binding experiment without unlabelled ligand (% Control).
  • Relative affinity values (IC 50 ) can be determined by fitting binding inhibition values by nonlinear regression, for example, by using Prizm data software.
  • the compound selected according to the processes and methods described herein is selected from the group consisting of:
  • enantiomers of compounds may both be selected using the criteria described herein or one may be selected and one not selected. Presumably, in certain situations, both enantiomers may be selected using the providec criteria.
  • the compound selected according to the processes and methods described herein is not an NMDA receptor channel blocker, such as, but not limited to FR 115427, NPS 1506, phencyclidine (PCP), remacemide, TCP, or EAA-090.
  • the compound selected according to the processes and methods described herein is not an NMDA receptor glutamate site antagonist, such as, but not limited to, CGP 40116, D-CPPene, GPI3000 (NPC 17742), MDL 100,453, or selfotel (CGS 19755).
  • the compound selected according to the processes and methods described herein is not an NMDA receptor glycine site antagonist, such as, but not limited to 7-Cl-kynurenate, HA966, MRZ 2/576, ZD9379, gavestinel (GVl 50526), andlicostinel (ACEA 1021, 5-nitro- 6,7-dichloro-l,4-dihydro-2,3-quinoxalinedione).
  • the compound does not exhibit substantial toxic side effects.
  • human NMDA receptors rather than other non-human species, the efficacy and potency boost of the compounds in vivo, in particular in human patients, can be effectively assessed using in vitro assays that allow minimization of toxic side effects before the compound enters an in vivo setting.
  • Toxic side effects include, but are not limited to, agitation, hallucination, confusion, stupor, paranoia, delirium, psychotomimetic-like symptoms, rotarod impairment, amphetamine-like stereotyped behaviors, stereotypy, psychosis memory impairment, motor impairment, anxiolytic-like effects, increased blood pressure, decreased blood pressure, increased pulse, decreased pulse, hematological abnormalities, electrocardiogram (ECG) abnormalities, cardiac toxicity, heart palpitations, motor stimulation, psychomotor performance, mood changes, short-term memory deficits, long-term memory deficits, arousal, sedation, extrapyramidal side-effects, ventricular tachycardia, lengthening of cardiac repolarisation, ataxia, cognitive deficits and/or schizophrenia-like symptoms.
  • ECG electrocardiogram
  • the compounds selected or identified according to the processes and methods described herein do not have substantial side effects associated with other classes of NMDA receptor antagonists.
  • such compounds do not substantially exhibit the side effects associated with NMDA antagonists of the glutamate site, such as, selfotel, D-CPPene (SDZ EAA 494) and AR-Rl 5896AR (ARL 15896AR), including, agitation, hallucination, confusion and stupor (Davis et al. (2000), Stroke 31(2):347-354; Diener et al. (2002), J Neurol 249(5):561-568); paranoia and delirium (Grotta et al.
  • such compounds do not exhibit the side effects associated with NMDA antagonists of the glycine site, such as HA-966, L-701,324, d- cycloserine, CGP-40116, and ACEA 1021, including significant memory impairment and motor impairment (Wlaz, P (1998), Brain Res Bull 46(6):535-540).
  • such compounds do not exhibit the side effects of NMDA receptor channel blockers, such as MK-801 and ketamine, including, psychosis-like effects (Hoffman, DC (1992), J Neural Transm Gen Sect 89:1-10); cognitive deficits (decrements in free recall, recognition memory, and attention; Malhotra et al (1996), Neuropsychopharmacology 14:301-307); schizophrenia-like symptoms (Krystal et al (1994), Arch Gen Psychiatry 51 : 199-214; Lahti et al. (2001), Neuropsychopharmacology 25:455-467), and hyperactivity and increased stereotpy (Ford et al (1989) Physiology and behavior 46: 755-758.
  • NMDA receptor channel blockers such as MK-801 and ketamine
  • the compound has a therapeutic index equal to or greater than at least 2: 1, at least 3: 1, at least 4: 1, at least 5: 1, at least 6:1, at least 7:1, at least 8:1, at least 9: 1, at least 10: 1, at least 15: 1, at least 20:1, at least 25:1, at least 30:1, at least 40:1, at least 50: 1, at least 75:1, at least 100: 1 or at least 1000: 1.
  • the therapeutic index can be defined as the ratio of the dose required to produce toxic or lethal effects to dose required to produce nonadverse or therapeutic response.
  • the median effective dose the dosage at which 50% of the population respond to the drug in a specific manner
  • the median toxic dose the dosage at which 50% of the group exhibits the adverse effect of the drug. The higher the therapeutic index, the more safe the drug is considered to be. It simply indicates that it would take a much higher dose to invoke a toxic response that it does to cause a beneficial effect.
  • the side effect profile of compounds can be determined by any method known to those skilled in the art.
  • motor impairment can be measured by, for example, measuring, locomotor activity and/or rotorod performance. Rotorod experiments involve measuring the duration that an animal can remain on an accelerating rod.
  • memory impairment can be assessed, for example, by using a passive avoidance paradigm; Sternberg memory scanning and paired words for short-term memory, or delayed free recall of pictures for long-term memory.
  • anxiolytic-like effects can be measured, for example, in the elevated plus maze task.
  • cardiac function can be monitored, blood pressure and/or body temperature measured and/or electrocardiograms conducted to test for side effects.
  • psychomotor functions and arousal can be measured, for example by analyzing critical flicker fusion threshold, choice reaction time, and/or body sway.
  • mood can be assessed using, for example, self-ratings.
  • schizophrenic symptoms can be evaluated, for example, using the PANSS, BPRS, and CGI, side-effects were assessed by the HAS and the S/A scale.
  • methods are provided to treat or prevent disorders that result in or are related to pH differences in tissues in patients by administering a compound selected according to the methods or processes described herein. Any disease, condition or disorder which induces a low pH can be treated according to the methods described herein.
  • methods to attenuate the progression of an ischemic, hypoxic or excitotoxic cascade associated with a drop in pH by administering an effective amount of a compound that exhibits the properties described herein are provided.
  • methods are provided to decrease infarct volume associated with a drop in pH by administering a compound that exhibits the properties described herein.
  • a method is provided to decrease cell death associated with a drop in pH by administering a compound that exhibits the properties described herein.
  • methods are provided to decrease behavioral deficits associated with an ischemic event associated with a drop in pH by administering a compound that exhibits the properties described herein.
  • methods are provided to treat patients with ischemic injury or hypoxia, or prevent or treat the neuronal toxicity associated with ischemic injury or hypoxia, by administering a compound selected according to the methods or processes described herein.
  • the ischemic injury can be stroke.
  • the ischemic injury can be selected from, but not limited to, one of the following: traumatic brain injury, cognitive deficit after bypass surgery, cognitive deficit after carotid angioplasty; and/ or neonatal ischemia following hypothermic circulatory arrest.
  • the ischemic injury can be vasospasm after subarachnoid hemorrhage.
  • a subarachnoid hemorrhage refers to an abnormal condition in which blood collects beneath the arachnoid mater, a membrane that covers the brain. This area, called the subarachnoid space, normally contains cerebrospinal fluid. The accumulation of blood in the subarachnoid space and the vasospasm of the vessels which results from it can lead to stroke, seizures, and other complications.
  • the methods and compounds described herein can be used to treat patients experiencing a subarachnoid hemorrhage.
  • the methods and compounds described herein can be used to limit the toxic effects of the subarachnoid hemorrhage, including, for example, stroke and/ or ischemia that can result from the subarachnoid hemorrhage.
  • the methods and compounds described herein can be used to treat patients with traumatic subarachnoid hemorrhage.
  • the traumatic subarachnoid hemorrhage can be due to a head injury.
  • the patients can have a spontaneous subarachnoid hemorrhage.
  • neuropathic pain or related disorders are provided to treat patients with neuropathic pain or related disorders by administering a compound selected according to the methods or processes described herein.
  • the neuropathic pain or related disorder can be selected from the group including, but not limited to: peripheral diabetic neuropathy, postherpetic neuralgia, complex regional pain syndromes, peripheral neuropathies, chemotherapy-induced neuropathic pain, cancer neuropathic pain, neuropathic low back pain, HIV neuropathic pain, trigeminal neuralgia, and/ or central post-stroke pain.
  • Neuropathic pain can be associated with signals generated ectopically and often in the absence of ongoing noxious events by pathologic processes in the peripheral or central nervous system. This dysfunction can be associated with common symptoms such as allodynia, hyperalgesia, intermittent abnormal sensations, and spontaneous, burning, shooting, stabbing, paroxysmal or electrical-sensations, paresthesias, hyperpathia and/or dysesthesias, which can also be treated by the compounds and methods described herein.
  • the compounds and methods described herin can be used to treat neuropathic pain resulting from peripheral or central nervous system pathologic events, including, but not limited to trauma, ischemia; infections or from ongoing metabolic or toxic diseases, infections or endocrinologic disorders, including, but not limited to, diabetes mellitus, diabetic neurophathy, amyloidosis, amyloid polyneuropathy (primary and familial), neuropathies with monoclonal proteins, vasculitic neuropathy, HIV infection, herpes zoster- shingles and/ or postherpetic neuralgia; neraopathy associated with Guillain-Barre syndrome; neuropathy associated with Fabry's disease; entrapment due to anatomic abnormalities; trigeminal and other CNS neuralgias; malignancies; inflammatory conditions or autoimmune disorders, including, but not limited to, demyelinating inflammatory disorders, rheumatoid arthritis, systemic lupus erythematosus, Sjogren's syndrome;
  • neuropathic pain can be treated according to the methods and compositions described herein include, but are not limited to, exposure to toxins or drugs (such as aresnic, thallium, alcohol, vincristine, cisplatinum and dideoxynucleosides), dietary or absorption abnormalities, immuno-globulinemias, hereditary abnormalities and amputations (including mastectomy).
  • toxins or drugs such as aresnic, thallium, alcohol, vincristine, cisplatinum and dideoxynucleosides
  • dietary or absorption abnormalities such as aresnic, thallium, alcohol, vincristine, cisplatinum and dideoxynucleosides
  • immuno-globulinemias such as aresnic, thallium, alcohol, vincristine, cisplatinum and dideoxynucleosides
  • immuno-globulinemias such as aresnic, thallium, alcohol, vincristine, c
  • methods are provided to treat patients with brain tumors by administering a compound selected according to the methods or processes described herein.
  • methods are provided to treat patients with neurodegenerative diseases by administering a compound selected according to the methods or processes described herein.
  • the neurodegenerative disease can be Parkinson's disease.
  • the neurodegenerative disease can be Alzheimer's, Huntington's and/ or Amyotrophic Lateral Sclerosis.
  • compounds selected according to the methods or processes described herein can be used prophylactically to prevent or protect against such diseases or neurological conditions, such as those described herein.
  • patients with a predisposition for an ischemic event such as a genetic predisposition
  • patients that exhibit vasospasms can be treated prophylactically with the methods and compounds described herein.
  • patients that have undergone cardiac bypass surgery can be treated prophylactically with the methods and compounds described herein.
  • chronic nerve injury such as, but not limited to diabetic neuropathy, ischemia, ischemia following transient or permanent vessel occlusion, seizures, spreading depression, restless leg syndrome, hypocapnia, hypercapnia, diabetic ketoacidosis, fetal asphyxia, spinal cord injury, traumatic brain injury, status epi ⁇ epticus, epilepsy, hypoxia, perinatal hypoxia, concussion, migraine, hypocapnia, hyperventilation, lactic acidosis, fetal asphyxia during parturition, brain gliomas, and/or retinopathies by administering a compound selected according to the methods or processes described herein.
  • chronic nerve injury such as, but not limited to diabetic neuropathy, ischemia, ischemia following transient or permanent vessel occlusion, seizures, spreading depression, restless leg syndrome, hypocapnia, hypercapnia, diabetic ketoacidosis, fetal asphyxia, spinal cord injury, traumatic brain injury, status epi ⁇ epticus, epile
  • Hosts including mammals and particularly humans, suffering from any of the disorders described herein, can be treated by administering to the host an effective amount of a compound described herein, or a pharmaceutically acceptable prodrug, ester, and/or salt thereof, optionally in combination with a pharmaceutically acceptable carrier or diluent.
  • the active compounds can be administered by any appropriate route, for example, orally, parenterally, intravenously, intradermally, intramuscularly, subcutaneously, sublingually, transdermally, bronchially, pharyngolaryngeal, intranasally, topically such as by a cream or ointment, rectally, intraarticular, intracisternally, intrathecally, intravaginally, intraperitoneally, intraocularly, by inhalation, bucally or as an oral or nasal spray.
  • parenterally intravenously, intradermally, intramuscularly, subcutaneously, sublingually, transdermally, bronchially, pharyngolaryngeal, intranasally, topically such as by a cream or ointment, rectally, intraarticular, intracisternally, intrathecally, intravaginally, intraperitoneally, intraocularly, by inhalation, bucally or as an oral or nasal spray.
  • the compounds of the present invention can be used in the form of pharmaceutically acceptable salts derived from inorganic or organic acids.
  • pharmaceutically acceptable salt is meant those salts which are, within the scope of sound medical judgment, suitable for use in contact with the tissues of humans and lower animals without undue toxicity, irritation, allergic response and the like and are commensurate with a reasonable benefit/risk ratio.
  • Pharmaceutically acceptable salts are well-known in the art. For example, P. H. Stahl, et al. describe pharmaceutically acceptable salts in detail in "Handbook of Pharmaceutical Salts: Properties, Selection, and Use” (Wiley VCH, Zurich, Switzerland: 2002).
  • the salts can be prepared in situ during the final isolation and purification of the compounds of the present invention or separately by reacting a free base function with a suitable organic acid.
  • Representative acid addition salts include, but are not limited to acetate, adipate, alginate, citrate, aspartate, benzoate, benzenesulfonate, bisulfate, butyrate, camphorate, camphorsufonate, digluconate, glycerophosphate, hemisulfate, heptanoate, hexanoate, fumarate, hydrochloride, hydrobromide, hydroiodide, 2-hydroxyethansulfonate (isethionate), lactate, maleate, methanesulfonate, nicotinate, 2-naphthalenesulfonate, oxalate, pamoate, pectinate, persulfate, 3-phenylpropionate, picrate, pivalate, propionate, succinate
  • the basic nitrogen-containing groups can be quaternized with such agents as lower alkyl halides such as methyl, ethyl, propyl, and butyl chlorides, bromides and iodides; dialkyl sulfates like dimethyl, diethyl, dibutyl and diamyl sulfates; long chain halides such as decyl, lauryl, myristyl and stearyl chlorides, bromides and iodides; arylalkyl halides like benzyl and phenethyl bromides and others. Water or oil-soluble or dispersible products are thereby obtained.
  • lower alkyl halides such as methyl, ethyl, propyl, and butyl chlorides, bromides and iodides
  • dialkyl sulfates like dimethyl, diethyl, dibutyl and diamyl sulfates
  • long chain halides such as decyl
  • acids which can be employed to form pharmaceutically acceptable acid addition salts include such inorganic acids as hydrochloric acid, hydrobromic acid, sulphuric acid and phosphoric acid and such organic acids as oxalic acid, maleic acid, succinic acid and citric acid.
  • Basic addition salts can be prepared in situ during the final isolation and purification of compounds of this invention by reacting a carboxylic acid-containing moiety with a suitable base such as the hydroxide, carbonate or bicarbonate of a pharmaceutically acceptable metal cation or with ammonia or an organic primary, secondary or tertiary amine.
  • a suitable base such as the hydroxide, carbonate or bicarbonate of a pharmaceutically acceptable metal cation or with ammonia or an organic primary, secondary or tertiary amine.
  • Pharmaceutically acceptable salts include, but are not limited to, cations based on alkali metals or alkaline earth metals such as lithium, sodium, potassium, calcium, magnesium and aluminum salts and the like and nontoxic quaternary ammonia and amine cations including ammonium, tetramethylammonium, tetraethylammonium, methylamine, dimethylamine, trimethylamine, triethylamine, diethylamine, ethylamine and the like.
  • Other representative organic amines useful for the formation of base addition salts include ethylenediamine, ethanolamine, diethanolamine, piperidine, piperazine and the like.
  • salts may be also obtained using standard procedures well known in the art, for example by reacting a sufficiently basic compound such as an amine with a suitable acid affording a physiologically acceptable anion.
  • a sufficiently basic compound such as an amine
  • a suitable acid affording a physiologically acceptable anion.
  • Alkali metal for example, sodium, potassium or lithium
  • alkaline earth metal for example calcium or magnesium
  • the formulations may conveniently be presented in unit dosage form and may be prepared by any of the methods well known in the art of pharmacy. All methods include the step of bringing into association a compound or a pharmaceutically acceptable salt or solvate thereof with the carrier which constitutes one or more accessory compounds. In general, the formulations are prepared by uniformly and intimately bringing into association the active compound with liquid carriers or finely divided solid carriers or both and then, if necessary, shaping the product into the desired formulation.
  • Solutions or suspensions used for parenteral, intradermal, subcutaneous, or topical application can include, for example, the following components: a sterile diluent such as water for injection, saline solution, fixed oils, polyethylene glycols, glycerine, propylene glycol or other synthetic solvents; antibacterial agents such as benzyl alcohol or methyl parabens; antioxidants such as ascorbic acid or sodium bisulfite; chelating agents such as ethylenediaminetetraacetic acid; buffers such as acetates, citrates or phosphates and agents for the adjustment of tonicity such as sodium chloride or dextrose.
  • the parental preparation can be enclosed in ampoules, disposable syringes or multiple dose vials made of glass or plastic. administered intravenously, preferred carriers are physiological saline
  • compositions of this invention for parenteral injection comprise pharmaceutically acceptable sterile aqueous or nonaqueous solutions, dispersions, suspensions or emulsions and sterile powders for reconstitution into sterile injectable solutions or dispersions.
  • suitable aqueous and nonaqueous carriers, diluents, solvents or vehicles include water, ethanol, polyols (propylene glycol, polyethylene glycol, glycerol, and the like), suitable mixtures thereof, vegetable oils (such as olive oil) and injectable organic esters such as ethyl oleate.
  • Proper fluidity may be maintained, for example, by the use of a coating such as lecithin, by the maintenance of the required particle size in the case of dispersions, and by the use of surfactants.
  • compositions may also contain adjuvants including preservative agents, wetting agents, emulsifying agents, and dispersing agents. Prevention of the action of microorganisms may be ensured by various antibacterial and antifungal agents, for example, parabens, chlorobutanol, phenol, sorbic acid, and the like. It may also be desirable to include isotonic agents, for example, sugars, sodium chloride and the like. Prolonged absorption of the injectable pharmaceutical form may be brought about by the use of agents delaying absorption, for example, aluminum monostearate and gelatin.
  • the absorption of the drug in order to prolong the effect of a drug, it is often desirable to slow the absorption of the drug from subcutaneous or intramuscular injection. This may be accomplished by the use of a liquid suspension of crystalline or amorphous material with poor water solubility. The rate of absorption of the drug then depends upon its rate of dissolution which, in turn, may depend upon crystal size and crystalline form. Alternatively, delayed absorption of a parenterally administered drug form is accomplished by dissolving or suspending the drug in an oil vehicle.
  • Suspensions in addition to the active compounds, may contain suspending agents, as, for example, ethoxylated isostearyl alcohols, polyoxyethylene sorbitol and sorbitan esters, microcrystalline cellulose, aluminum metahydroxide, bentonite, agar-agar, tragacanth, and mixtures thereof.
  • suspending agents as, for example, ethoxylated isostearyl alcohols, polyoxyethylene sorbitol and sorbitan esters, microcrystalline cellulose, aluminum metahydroxide, bentonite, agar-agar, tragacanth, and mixtures thereof.
  • the formulation compositions can also include adjuvants such as wetting agents, emulsifying and suspending agents, sweetening, flavoring, and perfuming agents.
  • adjuvants such as wetting agents, emulsifying and suspending agents, sweetening, flavoring, and perfuming agents.
  • the active compounds can also be in micro-or nano-encapsulated form, if appropriate, with one or more excipients.
  • Injectable depot forms are made by forming microencapsulated matrices of the drug in biodegradable polymers such as polylactide-polyglycolide. Depending upon the ratio of drug to polymer and the nature of the particular polymer employed, the rate of drug release can be controlled. Examples of other biodegradable polymers include poly(orthoesters) and poly(anhydrides). Depot injectable formulations are also prepared by entrapping the drug in liposomes or microemulsions which are compatible with body tissues.
  • the injectable formulations can be sterilized, for example, by filtration through a bacterial-retaining filter or by incorporating sterilizing agents in the form of sterile solid compositions which can be dissolved or dispersed in sterile water or other sterile injectable medium just prior to use.
  • Injectable preparations for example, sterile injectable aqueous or oleaginous suspensions may be formulated according to the known art using suitable dispersing or wetting agents and suspending agents.
  • the sterile injectable preparation may also be a sterile injectable solution, suspension or emulsion in a nontoxic, parenterally acceptable diluent or solvent such as a solution in 1,3-butanediol.
  • Suitable vehicles and solvents that may be employed are water, Ringer's solution, U.S.P. and isotonic sodium chloride solution.
  • sterile, fixed oils are conventionally employed as a solvent or suspending medium.
  • any bland fixed oil can be employed including synthetic mono- or diglycerides.
  • fatty acids such as oleic acid are used in the preparation of injectables.
  • Formulations for parenteral (including subcutaneous, intradermal, intramuscular, intravenous and intraarticular) administration include aqueous and non-aqueous sterile injection solutions which may contain anti-oxidants, buffers, bacteriostats and solutes which render the formulation isotonic with the blood of the intended recipient; and aqueous and non-aqueous sterile suspensions which may include suspending agents and thickening agents.
  • the formulations may be presented in unit-dose or multi-dose containers, for example sealed ampules and vials, and may be stored in a freeze-dried (lyophilized) condition requiring only the addition of the sterile liquid carrier, for example, saline, water- for-injection, immediately prior to use.
  • Extemporaneous injection solutions and suspensions may be prepared from sterile powders, granules and tablets of the kind previously described.
  • Another method of formulation of the present invention involves conjugating the compounds described herein to a polymer that enhances aqueous solubility.
  • suitable polymers include but are not limited to polyethylene glycol, poly-(d-glutamic acid), poly-(l -glutamic acid), poly-(l -glutamic acid), poly-(d-aspartic acid), poly-(l-aspartic acid), poly-(l-aspartic acid) and copolymers thereof.
  • Polyglutamic acids having molecular weights between about 5,000 to about 100,000 are preferred, with molecular weights between about 20,000 and 80,000 being more preferred and with molecular weights between about 30,000 and 60,000 being most preferred.
  • the polymer is conjugated via an ester linkage to one or more hydroxyls of an inventive epothilone using a protocol as essentially described by U.S. Pat. No. 5,977,163 which is incorporated herein by reference.
  • Preferred conjugation sites include the hydroxyl off carbon-21 in the case of 21 -hydroxy-derivatives of the present invention.
  • Other conjugation sites include but are not limited to the hydroxyl off carbon 3 and/or the hydroxyl off carbon 7.
  • the inventive compounds can be conjugated to a monoclonal antibody. This strategy allows the targeting of the inventive compounds to specific targets.
  • General protocols for the design and use of conjugated antibodies are described in "Monoclonal Antibody-Based Therapy of Cancer” (by Michael L. Grossbard, ed. (1998)).
  • the compounds of the invention are administered by any appropriate administration route, for example, orally, parenterally, intravenously, intradermally, intramuscularly, subcutaneously, sublingually, transdermally, bronchially, pharyngolaryngeal, intranasally, topically such as by a cream or ointment, rectally, intraarticular, intracisternally, intrathecally, intravaginally, intraperitoneally, intraocularly, by inhalation, bucally or as an oral or nasal spray.
  • the route of administration may vary, however, depending upon the condition and the severity of the diabetic vascular disease or ocular inflammation.
  • the precise amount of compound administered to a host or patient will be the responsibility of the attendant physician. However, the dose employed will depend on a number of factors, including the age and sex of the patient, the precise disorder being treated, and its severity.
  • a formulation for intravenous use can comprise an amount of an inventive compound ranging from about 1 mg/mL to about 25 mg/mL, preferably from about 5 mg/mL to 15 mg/mL, and more preferably about 10 mg/mL.
  • a dose range of from about 0.001 mg/kg per day to about 2500 mg/kg per day is typical.
  • the dose range is from about 0.1 mg/kg per day to about 1000 mg/kg per day.
  • the dose range is from about 0.1 mg/kg per day to about 500 mg/kg per day, including 1 mg/kg, 2 mg/kg, 5 mg/kg, 10 mg/kg, 15 mg/kg, 20 mg, kg, 25 mg/kg, 30 mg/kg, 35 mg/kg, 40 mg/kg, 45 mg/kg, 50 mg/kg, 100 mg/kg, 200 mg/kg, 300 mg/kg, 400 mg/kg, 500 mg/kg per day, and values between any two of the values given in this range.
  • the dose range for humans is generally from about 0.005 mg to 100 g/day.
  • the dose range in accordance with the present invention is such that the blood serum level of compounds of the present invention is from about 0.0 l ⁇ M to about 100 ⁇ M, and preferably from about 0.1 ⁇ M to about 100 ⁇ M.
  • Suitable values of blood serum levels in accordance with the present invention include but are not limited to about 0.01 ⁇ M, about 0.1 ⁇ M, about 0.5 ⁇ M, about 1 ⁇ M, about 5 ⁇ M, about 10 ⁇ M, about 15 ⁇ M, about 20 ⁇ M, about 25 ⁇ M, about 30 ⁇ M, about 35 ⁇ M, about 40 ⁇ M, about 45 ⁇ M, about 50 ⁇ M, about 55 ⁇ M, about 60 ⁇ M, about 65 ⁇ M, about 70 ⁇ M, about 75 ⁇ M, about 80 ⁇ M, about 85 ⁇ M, about 90 ⁇ M, about 95 ⁇ M and about 100 ⁇ M, as well as any blood serum level that falls within any two of these values (e.g, between about 10 ⁇ M and about 60
  • the compounds and formulations of the present invention can be administered in any of the known dosage forms standard in the art; in solid dosage form, semi-solid dosage form, or liquid dosage form, as well as subcategories of each of these forms.
  • Solid dosage forms for oral administration include capsules, caplets, tablets, pills, powders, lozenges, and granules.
  • the active compound is mixed with at least one inert, pharmaceutically acceptable excipient or carrier such as sodium citrate or dicalcium phosphate and/or a) fillers or extenders such as starches, lactose, sucrose, glucose, mannitol, and salicylic acid; b) binders such as carboxymethylcellulose, alginates, gelatin, polyvinylpyrrolidinone, sucrose, and acacia; c) humectants such as glycerol; d) disintegrating agents such as agar-agar, calcium carbonate, potato or tapioca starch, alginic acid, certain silicates, and sodium carbonate; e) solution retarding agents such as paraffin; f) absorption accelerators such as quaternary ammonium compounds; g) wetting agents such as cetyl alcohol and glycerol,
  • compositions of a similar type may also be employed as fillers in soft and hard- filled gelatin capsules using such excipients as lactose or milk sugar as well as high molecular weight polyethylene glycols and the like.
  • the solid dosage forms of tablets, capsules, pills, and granules can be prepared with coatings and shells such as enteric coatings and other coatings well known in the pharmaceutical formulating art. They may optionally contain opacifying agents and can also be of a composition that they release the active compound(s) only, or preferentially, in a certain part of the intestinal tract in a delayed manner. Examples of embedding compositions which can be used include polymeric substances and waxes.
  • a tablet may be made by compression or molding, optionally with one or more accessory compounds.
  • Compressed tablets may be prepared by compressing in a suitable machine the active compound in a free-flowing form such as a powder or granules, optionally mixed with a binder, lubricant, inert diluent, lubricating, surface active or dispersing agent. Molded tablets may be made by molding in a suitable machine a mixture of the powdered compound moistened with an inert liquid diluent. The tablets may optionally be coated or scored and may be formulated so as to provide slow or controlled release of the active compound therein.
  • compositions for rectal or vaginal administration are preferably suppositories which can be prepared by mixing the compounds of this invention with suitable non-irritating excipients or carriers such as cocoa butter, polyethylene glycol or a suppository wax which are solid at ambient temperature but liquid at body temperature and therefore melt in the rectum or vaginal cavity and release the active compound.
  • suitable non-irritating excipients or carriers such as cocoa butter, polyethylene glycol or a suppository wax which are solid at ambient temperature but liquid at body temperature and therefore melt in the rectum or vaginal cavity and release the active compound.
  • Semi-liquid dosage forms include those dosage forms that are too soft in structure to qualify for solids, but to thick to be counted as liquids. These include creams, pastes, ointments, gels, lotions, and other semisolid emulsions containing the active compound of the present invention.
  • Liquid dosage forms for oral administration include pharmaceutically acceptable emulsions, microemulsions, solutions, suspensions, syrups and elixirs.
  • the liquid dosage forms may contain inert diluents commonly used in the art such as, for example, water or other solvents, solubilizing agents and emulsifiers such as ethyl alcohol, isopropyl alcohol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butylene glycol, dimethylformamide, oils (in particular, cottonseed, groundnut, corn, germ, olive, castor, and sesame oils), glycerol, tetrahydrofurfuryl alcohol, polyethylene glycols and fatty acid esters of sorbitan, and mixtures thereof.
  • inert diluents commonly used in the art such as, for example, water or other solvents, solubilizing agents and
  • Formulations containing compounds of the invention may be administered through the skin by an appliance such as a transdermal patch.
  • Patches can be made of a matrix such as polyacrylamide, polysiloxanes, or both and a semi-permeable membrane made from a suitable polymer to control the rate at which the material is delivered to the skin.
  • Other suitable transdermal patch formulations and configurations are described in U.S. Pat. Nos. 5,296,222 and 5,271,940, as well as in Satas, D., et al, "Handbook of Pressure Sensitive Adhesive Technology, 2 nd Ed.”, Van Nostrand Reinhold, 1989: Chapter 25, pp. 627-642.
  • Powders and sprays can contain, in addition to the compounds of this invention, excipients such as lactose, talc, silicic acid, aluminum hydroxide, calcium silicates and polyamide powder, or mixtures of these substances.
  • Sprays can additionally contain customary propellants such as chlorofluorohydrocarbons.
  • excipients are described, for example, in "Handbook of Pharmaceutical Excipients, 3 rd Ed.”, A.H. Kibbe, Ed. (American Pharmaceutical Association and Pharmaceutical Press, Washington, DC, 2000), the entire contents of which are included herein by reference.
  • the active compounds of the present invention are prepared with carriers that will protect the compound against rapid elimination from the body or rapid release, such as a controlled release formulation, including implants and microencapsulated delivery systems.
  • a controlled release formulation including implants and microencapsulated delivery systems.
  • Biodegradable, biocompatible polymers can be used, such as ethylene vinyl acetate, polyanhydrides, polyglycolic acid, collagen, polyorthoesters, and polylacetic acid. Methods for preparation of such formulations will be apparent to those skilled in the art.
  • Example 1 Selectivity of Compound 93-4 for NMDA receptors versus other Glutamate receptors
  • Compound 93-4 series was shown to be selective for NMDA receptors by lack of effects on Xenopus oocytes injected with AMPA receptor and kainate receptor subunits. Glutamate or domoate induced current recordings were performed using two electrode voltage clamp, and 3 uM of Compound 93-4 coadministered with agonist (glutamate for AMPA receptors, domoate for kainate receptors). No reduction in the agonist induced response was seen, indicating that Compound 93-4 does not inhibit AMPA and kainate receptors. In addition, 3 uM of Compound 93-4 was effective at inhibiting NMDA receptor mediated currents when receptors are comprised of NR1/NR2B subunits but not NR1/NR2A or NR1/NR2D receptors.
  • (-) MK801 is at least 10-fold less potent than (+)MK801 in causing the induction of locomotor activity compared to vehicle injected control animals.
  • 3- 300 mg/kg 93-4, 3-300 mg/kg 93-5, 30-300 mg/kg of 93-8, 3-300 mg/kg of 93-31 ( Figure 5), 30 mg/kg of 93-40, and 30-300 mg/kg of 93-41 had no significant effects on locomotor activity.
  • Doses of 93 series compounds known to be neuroprotective do not have effects on locomotor activity.
  • Example 3 Determination of pH dependent potency shift in Xenopus oocytes.
  • cRNA was synthesized from linearized template cDNA for NMDA receptor subunits (NRl-Ia, NR2B, NR2A) according to manufacturer specifications (Ambion:). cDNAs used corresponded to GenBank numbers U08261 and Ul 1418 (NRl-Ia), AF001423 and CD13211 (NR2A), Ul 1419 (NR2B). Briefly, cDNA was linearized with an appropriate restriction enzyme downstream of the coding region, purified, and incubated with RNA polymerase and appropriate concentrations of ribonucleotides. In vitro transcribed cRNA was purified using standard methods.
  • Stage V and VI oocytes were surgically removed from the ovaries of large, well-fed and healthy Xenopus laevis anesthetized with 3-amino-benzoic acid ethyl ester (1 gm/1).
  • Clusters of oocytes were incubated with 292 U/ml Worthington (Freehold, NJ) type IV collagervase or 1.3 mg/ml collagenase (Life Technologies, Gaithersburg, MD; 17018-029) for 2 hr in Ca2+-free solution comprised of (in mM) 115 NaCl, 2.5 KCl, and 10 HEPES, pH 7.5, with slow agitation to remove the follicular cell layer.
  • Oocytes were then washed extensively in the same solution supplemented with 1.8 mM CaC12 and maintained in Barth's solution comprised of (in mM): 88 NaCl, 1 KCl, 24 NaHCO3, 10 HEPES, 0.82 MgSO4 , 0.33 Ca(NO3 )2, and 0.91 CaC12 and supplemented with 100 ug/ml gentamycin, 40 ug/ml streptomycin, and 50 ug/ml penicillin.
  • Oocytes were manually defolliculated and injected within 24 hr of isolation with 5 ng of NRl subunit and 10 ng of NR2 subunit in a 50 nl volume, and incubated in Barth's solution at 18°C for 3-7 d. Glass injection pipettes had tip sizes ranging from 10-20 microns, and were backfilled with mineral oil.
  • Preparation of pH-dependent NMDA receptor antagonists for testing. NMDA receptor antagonists were typically made up as 20 mM solutions in 100% DMSO and stored at -20C. This stock solution was sequentially diluted (1/10 v/v) to 2 mM, 0.2 mM, and 0.02 mM, all in 100% DMSO.
  • concentrations of drug tested were 0.01, 0.03 micromolar (diluting 0.02 mM stock into appropriate volumes), 0.1, 0.3 micromolar (diluting 0.2 mM stock into appropriate volumes), 1, 3 micromolar (diluting 2 mM stock into appropriate volumes), and/or 10, 30, 100 micromolar (diluting 20 mM stock into appropriate volumes).
  • Voltage-clamp recordings from Xenopus oocytes Two electrode voltage-clamp recordings were made 2-7 days post-injection. Oocytes were placed in a dual-track plexiglass recording chamber with a single perfusion line that splits in a Y-configuration to perfuse two oocytes. Dual recordings were made at room temperature using two Warner OC725B two- electrode voltage clamp amplifiers, arranged as recommended by the manufacturer. Glass microelectrodes (1-10 Megaohms) were filled with 300 mM KCl (voltage electrode) or 3 M KCl (current electrode). The bath clamps communicated across silver chloride wires placed into each side of the recording chamber, both of which were assumed to be at a reference potential of 0 mV.
  • Oocytes were perfused with a solution comprised of (in mM) 90 NaCl, 1 KCl, 10 HEPES, and 0.5 BaCl2, pH 7.3, and held at -40 mV.
  • Final concentrations for control application of glutamate (100 micromolar) plus glycine (50 micromolar) were achieved by adding appropriate volume from 100 and 30 mM stock solutions, respectively.
  • 10 micromolar final EDTA was obtained by adding a 1: 1000 dilution of 10 mM EDTA, in order to chelate contaminant divalent ions such as Zn2+. External pH was adjusted to either 6.9 or 7.6.
  • Dose response curves were obtained by applying in successive fashion maximal glutamate and glycine, followed by glutamate/glycine plus variable concentrations of antagonist. Dose response curves consisting of 4 to 6 concentrations were obtained in this manner.
  • the baseline leak current at -40 mV was measured before and after recording, and the full recording linearly corrected for any change in leak current.
  • Oocytes with glutamate- evoked responses smaller than 100 nA at pH 7.6 or 50 nA at pH 6.9 were not included.
  • the level of inhibition by applied antagonist was expressed as a percent of the initial glutamate response, and averaged together across oocytes from a single frog. Each experiment consisted of recordings at each pH from 3 to 10 oocytes obtained from a single frog.
  • the average percent responses at each of 4 to 8 antagonist concentrations were fitted by the logistic equation, (100-min)/(l+([conc]/IC50) nH ) + min, where min is the residual percent response in saturating antagonist, IC50 is the concentration of antagonist that causes half of the achievable inhibition, and nH is a slope factor describing steepness of the inhibitory curve.
  • Min was constrained to be greater than or equal to 0.
  • min was set to 0.
  • the IC50 values obtained at pH 7.6 and 6.9 were expressed as a ratio and averaged together to determine the mean shift in IC50.
  • Example 4 Determination of neuroprotection in an in vivo model of transient focal ischemia
  • Transient Focal Ischemia was induced by intraluminal middle cerebral artery (MCA) occlusion with a monofilament suture. Briefly, male C57BL/6 mice (3-5 months old, The Jackson Laboratory) were anesthetized with 2% isofiurane in 98% 02. The rectal temperature was controlled at 37°C (range 36.5-37.5) with a homeothermic blanket. Relative changes in regional cerebral blood flow were monitored with a laser Doppler flowmeter (Perimed). To do this the probe was glued directly to the skull 2 mm posterior and 4-6 mm lateral of the bregma.
  • the infarct area of each section was measured using NIH IMAGE (Scion Corporation, Beta 4.0.2 release) and multiplied by the section thickness to give the infarct volume of that section.
  • the density slice option in NIH IMAGE was used to segment the images based on the intensity determined as 70% or 75% of that in the contralateral undamaged cortex. This standard was maintained throughout the analysis in all animals, and only objects at this intensity were highlighted for area measurement.
  • the area of the lesion, as identified by digitally identified threshold reductions in TTC staining, was manually outlined. A ratio of the contralateral to ipsilateral hemisphere section volume was multiplied by the corresponding infarct section volume to correct for edema.
  • Infarct volume was determined by summing the infarct area times section thickness for all sections. At least 12 animals were included in each measurement. For some experiments, the regions of damage were directly measreud by circling freehand the region of reduced staining. Identical results were obtained with the two procedures.
  • Intraperitoneal administration of pH-dependent NMDA receptor antagonists C57B1/6 mice received an intraperitoneal (IP) injection of 93-4, 93-5, 93-8, 93-31, 93-40 30 min before MCA occlusion surgery.
  • IP intraperitoneal
  • a 30 mg/ml stock solution in 50% DMSO was prepared by adding 30 mg of compound into 0.5 ml of DMSO followed by addition of 0.5 ml of 0.9% saline with vortexing.
  • the working solution for the IP injection solution was 3 mg/ml in 0.9% saline (50% v/v DMSO), and was prepared by transferring 0.2 ml of the stock solution into a new tube and adding 0.9 ml of DMSO and 0.9 ml of 0.9% saline with vortexing. 3-30 mg/kg final dose was administered to mice, with injection volume varying depending on animal weight and desired dose.
  • mice received a small volume intracerebroventricular (ICV) injection of NMDA antagonist (93-5, 93-97, 93-31, 93-41, 93-43) or appropriate vehicle prior to surgery. Initially a 20 mM stock solution in 100% DMSO was prepared for all drugs. Five microliters of this stock solution was transferred to a new tube and 45 microliters of DMSO added for drugs 93-41, 93-43 with vortexing.
  • ICV intracerebroventricular
  • phosphate buffered saline PBS, 0.9% NaCl, pH 7.4, Sigma 1000-3
  • PBS phosphate buffered saline
  • 5 microliters of 20 mM DMSO stock solution was transferred to a new tube and 15 ul of DMSO added with vortexing.
  • To this solution 180 microliters of PBS was added to give a working solution of 0.5 mM drug in 10% v/v DMSO.
  • DMSO was substituted for 20 mM drag in DMSO.
  • mice All ICV injections were made into the right ventricle (2 mm posterior and 1 mm lateral of the bregma, needle inserted 3 mm) of male C57BL/6 mice (3-5 months old, The Jackson Laboratory) 30 min before MCA occlusion surgery. Mice were killed 24 h after MCA occlusion surgery and the lesion was identified and analyzed as described above. Mice with subarachnoid hemorrhage were identified by appearance of blood clot in excess of ⁇ 1 mm at base of skull, and were excluded. Results
  • Figure 2 illustrates the comparison of the in vitro potency boost of Compounds 93-97, 93-43, 93-5, 93-41, and 93-31 at pH 6.9 vs 7.6 versus tissue infarct volume following ICV administration of these agents.
  • the data represents the % of infarct volume determined for vehicle injected controls and potency boost measured as described above.
  • the grey shadowed area indicates the area which defines the identified bounds of the criteria for superior drug performance.
  • the drugs which fall within the bounds are those that have a mean (not error bars) within the grey blocked area.
  • the infarct volume was measured in C57B1/6 mice following a transient focal ischemic event as described above for each compound.
  • Compounds 93-97, 93-43, 93-5, 93- 41 and 93-31 were applied intracerebroventricularly (ICV; solid circles) as described above. Error bars are standard error of the mean (SEM).
  • the potency boosts at pH 6.9 vs 7.6 for Compounds 93-5, 93-31, 93-41, 93-43, and 93-97 were calculated as described herein for oocytes expressing NR1/NR2B receptors.
  • Figure 3 illustrates the comparison of the in vitro potency boost of Compounds 93-4, 93-5, 93-8, 93-31, 93-40 at pH 6.9 vs 7.6 versus tissue infarct volume.
  • the data represents the actual infarct volume expressed as percent of that in vehicle injected control animals and potency boost was calculated as described above.
  • the grey shadowed area indicates the area which defines the identified bounds of the criteria for superior drug performance.
  • the drugs which fall within the bounds are those that have a mean (not error bars) within the grey blocked area.
  • infarct volume was measured in C57B1/6 mice following a transient focal ischemic event as described above for each compound.
  • Drug was applied by intraperitoneal injection (IP) as described above. Error bars are SEM.
  • Infarct volume was inferred from the percent reduction in infarct volume for IP administration compared to paired controls. This was calculated as the product of the infarct volume expressed as percent of control infarct induced by drug in an independent experiment and the mean control infarct volume (mm3) for ICV experiments, which is shown as solid line (broken lines show mean control infarct+- SEM).
  • Figure 4 compares the in vitro potency boost at NR1/NR2A and NR1/NR2B of known compounds at pH 6.9 vs 7.6 versus percent control tissue infarct volume.
  • the grey shadowed area indicates the area which defines the identified bounds of the criteria for superior drug performance.
  • the drugs which fall within the bounds are those that have a mean (not error bars) within the grey blocked area.
  • the potency boosts at pH 6.9 vs 7.6 for all compounds were calculated as described above for oocytes expressing either NR1/NR2A or NR1/NR2B receptors (see Table 3 and 4 for summary of numbers of experiments).
  • the pH boost for ifenprodil (IF), CP 101,606 (CP) were determined from them literature (Mott et al., 1998).
  • mice examined for infarct volume The number of mice examined for infarct volume is shown in Table 3.
  • potency boost measurements on NRl-la/NR2B receptors the number of frogs used and the largest number of oocytes tested at a single concentration at pH 6.9 and pH 7.6 are shown in Table 3.
  • IC50 at each pH multiple concentrations of each drug were tested.
  • potency boost measurements on NRl-la/NR2A receptors the number of frogs used and the largest number of oocytes tested at a single concentration at pH 6.9 and pH 7.6 are shown in Table 4.
  • Table 3 Number of repetitions of each experiment for data from NR1/NR2B presented in Figures 1,2,3,4.
  • Table 4 Number of repetitions of each experiment for data from NRl/NR2A presented in Figure 4.
  • Figure 1 represents a composite of Figures 2, 3 and 4. It illustrates that of the 24 compounds tested, 20 compound (83%) fall outside the area of the invention (denoted by the shaded area), indicating that over 80% of compounds tested fail to meet the identified standard for superior in vivo therapy.
  • the grey shadowed area indicates the area that defines the identified bounds of the criteria for superior drug performance.
  • the drugs which fall within the bounds are those that have a mean (not error bars) within the grey blocked area.
  • the mean of Compounds 93-4, 93-5, 93-41, 93-31 fall within the shaded area for NR1/NR2B.
  • the mean of (-) MK801and ketamine fall within the shaded area for NR1/NR2A ( Figure 4).
  • infarct volume was measured in C57B1/6 mice following a transient focal ischemic event as described above for compounds indicated by symbols.
  • Drug was applied intracerebroventricularly (ICV; squares) or by intraperitoneal injection (IP; circles) as described above. Error bars are SEM.
  • Infarct volume was directly measured as percent of the control infarct volume for IP administration compared to paired controls. Control is shown as solid line (broken lines show mean control infarct +/- SEM).
  • Open symbols show the reduction in infarct volume by administration of CNSl 102 (CN, aptiganel or Cerestat, Dawson et al., 2001), dextromethorphan (DM, Steinberg et al., 1995), dextrorphan (DX; Steinberg et al., 1995), levomethorphan (LM; Steinberg et al., 1995), (S) ketamine (KT; Proescholdt et al., 2001), memantine (MM; Culmsee et al. 2004), ifenprodil (IF, Dawson et al. 2001), CP 101,606 (CP; Yang et al.
  • Example 5 Evaluation in an in vivo model of neuropathic pain Methods
  • mice Male Sprague-Dawley rats (Hsd:Sprague-Dawley® TM SD® TM , Harlan, Indianapolis, Indiana, U.S.A.) weighing 100 ⁇ 10 g on surgery day and 250 ⁇ 10 g on testing day were housed three per cage. Animals had free access to food and water and were maintained on a 12:12h light/dark schedule. The animal colony was maintained at 21 0 C and 60% humidity. All experiments were conducted in accordance with the International Association for the Study of Pain guidelines and were approved by the University of Minnesota Animal Care and Use Committee.
  • Drugs and dosing solutions The drugs were dissolved in 1 % v/v DMSO and 66% v/v PEG 400 in distilled water. Compounds were administered by i.p. route.
  • Compound 93-31 appeared to attenuate mechanical allodynia in the SNL model of neuropathic pain when administered i.p. at 100 mg/kg.
  • Compound 93-97 failed to attenuate mechanical allodynia in SNL rats at the doses tested (30 and 100 mg/kg) in this study.
  • Compound 93-31 (100 mg/kg) appeared to have a faster onset (30 min) and shorter duration of action (60 min) than did the reference compound gabapentin (100 mg/kg).
  • the peak threshold observed in animals treated with the 100 mg/kg dose of Compound 93-31 was approximately half of that seen in the normal paw. Assuming complete reversal may be achieved with higher doses of Compound 93-31, this suggests the ED50 is approximately 100 mg/kg.
  • Example 6 pH Dependence of Selected Compound A series of n-alkyl derivatives were tested for pH dependence.
  • Example 7 Human vs. rat receptor cDNA in determining the IC50 for antagonism of NMD A-NR2B containing receptors in the Xenopus oocyte assay.

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Abstract

L'invention concerne des procédés pour l'identification d'un composé qui est utile pour traiter ou prévenir un trouble qui réduit le pH dans une région d'un tissu affecté comprenant l'évaluation de la différence de pouvoir, ou augmentation de pouvoir, du composé à un pH physiologique par rapport à un pH induit par un trouble dans une cellule qui exprime un récepteur de NMDA humain. L'évaluation de l'augmentation de pouvoir peut comprendre la mesure d'une CI50 d'un composé à un pH physiologique et à un pH induit par un trouble (« l'augmentation de pouvoir ») jusqu'à ce qu'un intervalle de confiance de 95 % pour l'augmentation de pouvoir ne change pas de plus de 15 % avec l'ajout d'une nouvelle expérience, où les mesures sont répétées au moins 5 fois. Les procédés peuvent être utilisés pour la sélection d'antagonistes du récepteur de NMDA sûrs pour le traitement ou la prévention d'un trouble humain qui réduit le pH dans une région d'un tissu affecté. De tels troubles comprennent, sans s'y limiter, la douleur neuropathique, l'ischémie, la maladie de Parkinson, l'épilepsie et les lésions cérébrales traumatiques.
PCT/US2008/082660 2007-11-06 2008-11-06 Procédés d'identification d'antagonistes du récepteur de nmda sûrs WO2009061935A2 (fr)

Priority Applications (11)

Application Number Priority Date Filing Date Title
EA201070571A EA201070571A1 (ru) 2007-11-06 2008-11-06 Улучшенные способы идентификации антагонистов nmda-рецептора
CN2008801240927A CN101918832A (zh) 2007-11-06 2008-11-06 鉴定安全的nmda受体拮抗剂的方法
CA2704475A CA2704475A1 (fr) 2007-11-06 2008-11-06 Procedes d'identification des antagonistes des recepteurs de nmda ameliores
AU2008323877A AU2008323877A1 (en) 2007-11-06 2008-11-06 Methods of identifying safe NMDA receptor antagonists
EP08847388A EP2212694A4 (fr) 2007-11-06 2008-11-06 Procédés d'identification d'antagonistes du récepteur de nmda sûrs
JP2010532343A JP2011503013A (ja) 2007-11-06 2008-11-06 安全なｎｍｄａ受容体拮抗薬を同定する方法
BRPI0820406-3A BRPI0820406A2 (pt) 2007-11-06 2008-11-06 Métodos para identificar aumento de antagonistas do receptor nmda
MX2010004971A MX2010004971A (es) 2007-11-06 2008-11-06 Metodos de identificacion de antagonistas seguros del receptor nmda.
IL205432A IL205432A0 (en) 2007-11-06 2010-04-29 Method of identigfying safe nmda receptor antagonists
US12/772,798 US20100272648A1 (en) 2008-11-06 2010-05-03 Methods of Identifying Improved NMDA Receptor Antagonists
ZA2010/03724A ZA201003724B (en) 2007-11-06 2010-05-25 Methods of identifying safe nmda receptor antagonists

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CN111960514A (zh) * 2020-08-28 2020-11-20 浙江浙能技术研究院有限公司 一种用于脱硫废水浓缩的电渗析智能控制系统及方法

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