WO2009060961A1 - ウエストナイルウイルスワクチンおよびその製造方法 - Google Patents

ウエストナイルウイルスワクチンおよびその製造方法 Download PDF

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Publication number
WO2009060961A1
WO2009060961A1 PCT/JP2008/070354 JP2008070354W WO2009060961A1 WO 2009060961 A1 WO2009060961 A1 WO 2009060961A1 JP 2008070354 W JP2008070354 W JP 2008070354W WO 2009060961 A1 WO2009060961 A1 WO 2009060961A1
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WO
WIPO (PCT)
Prior art keywords
wnv
vlp
secreting
nucleic acid
acid encoding
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PCT/JP2008/070354
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English (en)
French (fr)
Inventor
Asato Kojima
Hidehiro Takahashi
Toyokazu Ishikawa
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Japan As Represented By The Director-General Of National Institute Of Infectious Diseases
The Research Foundation For Microbial Diseases Of Osaka University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Japan As Represented By The Director-General Of National Institute Of Infectious Diseases, The Research Foundation For Microbial Diseases Of Osaka University filed Critical Japan As Represented By The Director-General Of National Institute Of Infectious Diseases
Priority to CA2704556A priority Critical patent/CA2704556A1/en
Priority to US12/741,479 priority patent/US8795687B2/en
Priority to AU2008325537A priority patent/AU2008325537B2/en
Priority to EP08846317.9A priority patent/EP2221368B1/en
Priority to JP2009540105A priority patent/JP5448836B2/ja
Priority to CN200880115257A priority patent/CN101855349A/zh
Publication of WO2009060961A1 publication Critical patent/WO2009060961A1/ja
Priority to HK11101725.7A priority patent/HK1147773A1/xx
Priority to US14/070,948 priority patent/US20140065180A1/en

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/12—Viral antigens
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/7088—Compounds having three or more nucleosides or nucleotides
    • A61K31/711—Natural deoxyribonucleic acids, i.e. containing only 2'-deoxyriboses attached to adenine, guanine, cytosine or thymine and having 3'-5' phosphodiester links
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A61P31/14—Antivirals for RNA viruses
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
    • A61K2039/525—Virus
    • A61K2039/5258—Virus-like particles
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/24011—Flaviviridae
    • C12N2770/24111—Flavivirus, e.g. yellow fever virus, dengue, JEV
    • C12N2770/24122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/24011—Flaviviridae
    • C12N2770/24111—Flavivirus, e.g. yellow fever virus, dengue, JEV
    • C12N2770/24134—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Virology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Epidemiology (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oncology (AREA)
  • Communicable Diseases (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

　本発明は、ウエストナイルウイルス（ＷＮＶ）由来のシグナル配列を改変したウイルス様粒子（ＶＬＰ）高分泌生産シグナルペプチド、該シグナルペプチド、ｐｒＭ蛋白質およびＥ蛋白質をコードする核酸を含むＷＮＶのＶＬＰ分泌発現ベクター、該ベクターを導入したＷＮＰのＶＬＰ高分泌生産動物細胞株、該細胞株によって得られるＷＮＶのＶＬＰを有効成分とするＷＮＶワクチン、並びに上記ＶＬＰ分泌発現ベクターを有効成分とするＷＮＶのＤＮＡワクチンを提供する。
PCT/JP2008/070354 2007-11-04 2008-11-07 ウエストナイルウイルスワクチンおよびその製造方法 WO2009060961A1 (ja)

Priority Applications (8)

Application Number Priority Date Filing Date Title
CA2704556A CA2704556A1 (en) 2007-11-07 2008-11-07 West nile virus vaccine, and method for production thereof
US12/741,479 US8795687B2 (en) 2007-11-07 2008-11-07 West Nile virus prM-E signal peptide facilitating the efficient assembly, maturation, and release of virus-like particles (VLPs)
AU2008325537A AU2008325537B2 (en) 2007-11-07 2008-11-07 West nile virus vaccine, and method for production thereof
EP08846317.9A EP2221368B1 (en) 2007-11-07 2008-11-07 West nile virus vaccine, and method for production thereof
JP2009540105A JP5448836B2 (ja) 2007-11-07 2008-11-07 ウエストナイルウイルスワクチンおよびその製造方法
CN200880115257A CN101855349A (zh) 2007-11-07 2008-11-07 西尼罗病毒疫苗及其生产方法
HK11101725.7A HK1147773A1 (en) 2007-11-07 2011-02-22 West nile virus vaccine, and method for production thereof
US14/070,948 US20140065180A1 (en) 2007-11-04 2013-11-04 West nile virus vaccine, and method for production thereof

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007-290169 2007-11-07
JP2007290169 2007-11-07

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US12/741,479 A-371-Of-International US8795687B2 (en) 2007-11-07 2008-11-07 West Nile virus prM-E signal peptide facilitating the efficient assembly, maturation, and release of virus-like particles (VLPs)
US14/070,948 Division US20140065180A1 (en) 2007-11-04 2013-11-04 West nile virus vaccine, and method for production thereof

Publications (1)

Publication Number Publication Date
WO2009060961A1 true WO2009060961A1 (ja) 2009-05-14

Family

ID=40625846

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2008/070354 WO2009060961A1 (ja) 2007-11-04 2008-11-07 ウエストナイルウイルスワクチンおよびその製造方法

Country Status (9)

Country Link
US (2) US8795687B2 (ja)
EP (1) EP2221368B1 (ja)
JP (1) JP5448836B2 (ja)
CN (1) CN101855349A (ja)
AU (1) AU2008325537B2 (ja)
CA (1) CA2704556A1 (ja)
HK (1) HK1147773A1 (ja)
SG (1) SG188127A1 (ja)
WO (1) WO2009060961A1 (ja)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106754982A (zh) * 2016-12-14 2017-05-31 中国农业科学院哈尔滨兽医研究所 表达绿色荧光蛋白的限制性复制西尼罗病毒系统及其应用

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102183637A (zh) * 2011-01-25 2011-09-14 中国检验检疫科学研究院 检测人西尼罗病毒IgG抗体的间接ELISA试剂盒及其检测方法
US11434261B2 (en) 2016-07-27 2022-09-06 Hawaii Biotech Inc. Optimized Zika virus envelope gene and expression thereof
RU2642312C1 (ru) * 2016-09-20 2018-01-24 Федеральное государственное бюджетное военное образовательное учреждение высшего образования Военно-медицинская академия им. С.М. Кирова Министерства обороны Российской Федерации (ВМедА) Способ профилактики и лечения опасных нейровирусных инфекций
CN108950086A (zh) * 2018-08-29 2018-12-07 浙江国际旅行卫生保健中心（浙江出入境检验检疫局口岸门诊部） 基于raa荧光法检测西尼罗病毒的引物探针组及检测试剂盒

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JP2004065118A (ja) 2002-08-07 2004-03-04 Handai Biseibutsubiyou Kenkyukai 日本脳炎ウイルス抗原及びその製造方法

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AU2002211490A1 (en) * 2000-10-04 2002-04-15 The Trustees Of The University Of Pennsylvania Compositions and methods of using capsid protein from flaviviruses and pestiviruses
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WO2007103565A2 (en) * 2006-03-09 2007-09-13 The Government Of The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Wnv dna vaccine incorporating a cmv/r backbone and expressing wnv prm and e proteins

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106754982A (zh) * 2016-12-14 2017-05-31 中国农业科学院哈尔滨兽医研究所 表达绿色荧光蛋白的限制性复制西尼罗病毒系统及其应用
CN106754982B (zh) * 2016-12-14 2021-03-09 中国农业科学院哈尔滨兽医研究所 表达绿色荧光蛋白的限制性复制西尼罗病毒系统及其应用

Also Published As

Publication number Publication date
JPWO2009060961A1 (ja) 2011-03-24
AU2008325537A1 (en) 2009-05-14
EP2221368A4 (en) 2010-11-24
CN101855349A (zh) 2010-10-06
US20100247559A1 (en) 2010-09-30
US20140065180A1 (en) 2014-03-06
SG188127A1 (en) 2013-03-28
AU2008325537B2 (en) 2014-07-24
HK1147773A1 (en) 2011-08-19
JP5448836B2 (ja) 2014-03-19
CA2704556A1 (en) 2009-05-14
US8795687B2 (en) 2014-08-05
EP2221368B1 (en) 2014-03-26
EP2221368A1 (en) 2010-08-25

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