WO2009037950A1 - ヒト変異型K-ras（K-rasV12）遺伝子をコンディショナルに発現するトランスジェニック非ヒト哺乳動物及びその用途 - Google Patents

ヒト変異型K-ras（K-rasV12）遺伝子をコンディショナルに発現するトランスジェニック非ヒト哺乳動物及びその用途 Download PDF

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Publication number
WO2009037950A1
WO2009037950A1 PCT/JP2008/065379 JP2008065379W WO2009037950A1 WO 2009037950 A1 WO2009037950 A1 WO 2009037950A1 JP 2008065379 W JP2008065379 W JP 2008065379W WO 2009037950 A1 WO2009037950 A1 WO 2009037950A1
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WIPO (PCT)
Prior art keywords
human
transgenic non
rasv12
gene
human mammal
Prior art date
Application number
PCT/JP2008/065379
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English (en)
French (fr)
Inventor
Hiroyuki Tsuda
Katsumi Fukamachi
David B. Alexander
Original Assignee
Nagoya City University
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Publication date
Application filed by Nagoya City University filed Critical Nagoya City University
Priority to JP2009533096A priority Critical patent/JP5761914B2/ja
Publication of WO2009037950A1 publication Critical patent/WO2009037950A1/ja

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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/82—Translation products from oncogenes
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/20—Animal model comprising regulated expression system
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/03—Animal model, e.g. for test or diseases
    • A01K2267/0331—Animal model for proliferative diseases

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Environmental Sciences (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Oncology (AREA)
  • Veterinary Medicine (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

　短期間で所望の組織・器官に癌を発生させることが可能なモデル動物（非ヒト哺乳動物）及びその用途を提供することを課題とする。部位特異的組換え酵素の作用を受けたときにヒト変異型K-ras（K-rasV12）遺伝子を発現する発現カセットがゲノム中に組み込まれているトランスジェニック非ヒト哺乳動物が提供される。また、当該トランスジェニック非ヒト哺乳動物を用いたスクリーニング方法が提供される。
PCT/JP2008/065379 2007-09-20 2008-08-28 ヒト変異型K-ras（K-rasV12）遺伝子をコンディショナルに発現するトランスジェニック非ヒト哺乳動物及びその用途 WO2009037950A1 (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2009533096A JP5761914B2 (ja) 2007-09-20 2008-08-28 ヒト変異型Ｋ−ｒａｓ（Ｋ−ｒａｓＶ１２）遺伝子をコンディショナルに発現するトランスジェニック非ヒト哺乳動物及びその用途

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007-243313 2007-09-20
JP2007243313 2007-09-20

Publications (1)

Publication Number Publication Date
WO2009037950A1 true WO2009037950A1 (ja) 2009-03-26

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PCT/JP2008/065379 WO2009037950A1 (ja) 2007-09-20 2008-08-28 ヒト変異型K-ras（K-rasV12）遺伝子をコンディショナルに発現するトランスジェニック非ヒト哺乳動物及びその用途

Country Status (2)

Country Link
JP (1) JP5761914B2 (ja)
WO (1) WO2009037950A1 (ja)

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2005503143A (ja) * 2001-07-27 2005-02-03 ワイス Ｋｖベータ１．１サブユニットの非機能性Ｎ末端を含んでいるノックイントランスジェニック哺乳動物

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2372542A1 (en) * 2001-02-20 2002-08-20 Pfizer Products Inc. Transgenic animals containing a dominant negative mutant form of the p85 subunit of pi-3 kinase

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2005503143A (ja) * 2001-07-27 2005-02-03 ワイス Ｋｖベータ１．１サブユニットの非機能性Ｎ末端を含んでいるノックイントランスジェニック哺乳動物

Non-Patent Citations (9)

* Cited by examiner, † Cited by third party
Title
"31st Annual Meeting Society for Neuroscience's, Society for Neuroscience Abstract", 2001, article KWAK, S.P. ET AL.: "Creation and characterization of KVbeta1.1 knock-in mice lacking the N-terminus necessary for rapid inactivation" *
DREISSIGACKER, U. ET AL.: "Oncogenic K-Ras down-regulates Rac1 and RhoA activity and enhances migration and invasion of pancreatic carcinoma cells through activation of p38", CELL.SIGNAL., vol. 18, 2006, pages 1156 - 1168 *
GUERRA, C. ET AL.: "Tumor induction by an endogenous K-ras oncogene is highly dependent on cellular context.", CANCER CELL, vol. 4, August 2003 (2003-08-01), pages 111 - 120 *
HAMEYER, D. ET AL.: "Toxicity of ligand- dependent Cre recomninases and generation of a conditional Cre deleter mouse allowing mosaic recombination in peripheral tissues.", PHYSIOL. GENOM., vol. 31, 24 April 2007 (2007-04-24), pages 32 - 41 *
LUO, F. ET AL.: "Conditional expression of mutated K-ras accelerates intestinal tumorigenesis in Msh2-deficient mice", ONCOGENE, vol. 26, no. 3, 5 February 2007 (2007-02-05), pages 4415 - 4427 *
SANSOM, O.J. ET AL.: "Loss of Apc allows phenotypic manifestation of the transforming properties of an endogenous K-ras oncogene in vivo", PROC.NATL.ACAD.SCI.USA, vol. 103, no. 38, 19 September 2006 (2006-09-19), pages 14122 - 14127 *
SHEN, S. ET AL.: "Overexpression of the human VPAC2 receptor in the suprachiasmatic nucleus alters the circadian phenotype of mice.", PROC.NATL.ACAD.SCI.USA, vol. 97, no. 21, 2000, pages 11575 - 11580 *
SHONOBU UEDA ET AL.: "Hito Hen'igata c-Ha-ras Conditional Transgenic Rat ni Okeru Hatsugansei no Kento", DAI 92 KAI JAPANESE SOCIETY OF PATHOLOGY SOKAI YOKOSHU, 4 April 2003 (2003-04-04), pages 321 *
UEDA, S. ET AL.: "Ductal origin of pancreatic adenocarcinomas induced by conditional activation of a human Ha-ras oncogene in rat pancreas", CARCINOGENESIS, vol. 27, no. 12, 2006, pages 2497 - 2510 *

Also Published As

Publication number Publication date
JP5761914B2 (ja) 2015-08-12
JPWO2009037950A1 (ja) 2011-01-06

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