WO2009037839A1 - 細胞内に存在する被検物質の検出方法および検出キット - Google Patents

細胞内に存在する被検物質の検出方法および検出キット Download PDF

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Publication number
WO2009037839A1
WO2009037839A1 PCT/JP2008/002574 JP2008002574W WO2009037839A1 WO 2009037839 A1 WO2009037839 A1 WO 2009037839A1 JP 2008002574 W JP2008002574 W JP 2008002574W WO 2009037839 A1 WO2009037839 A1 WO 2009037839A1
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WIPO (PCT)
Prior art keywords
cell
substance
incorporated
labeled
detection
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PCT/JP2008/002574
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English (en)
French (fr)
Inventor
Kenichi Osada
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St. Marianna University School Of Medicine
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Priority to JP2009533049A priority Critical patent/JPWO2009037839A1/ja
Publication of WO2009037839A1 publication Critical patent/WO2009037839A1/ja

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
    • G01N33/5035—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects on sub-cellular localization

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  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Toxicology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

　本発明は、細胞内に取り込まれた被検物質を詳細かつ簡便に検出できる検出方法に関する。アミノ基またはイミノ基を有する被検物質をＮＢＤ－Ｆ（４－フルオロ－７－ニトロベンゾフラザン）で標識し、標識物質を細胞内に取り込ませる。細胞内に取り込まれた標識物質からの蛍光を検出することで、細胞内に取りこまれた被検物質を詳細かつ簡便に観察することができる。本発明は、例えば、薬物や神経伝達物質などの作用機序の研究などに有用である。
PCT/JP2008/002574 2007-09-18 2008-09-18 細胞内に存在する被検物質の検出方法および検出キット WO2009037839A1 (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2009533049A JPWO2009037839A1 (ja) 2007-09-18 2008-09-18 細胞内に存在する被検物質の検出方法および検出キット

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007241010 2007-09-18
JP2007-241010 2007-09-18

Publications (1)

Publication Number Publication Date
WO2009037839A1 true WO2009037839A1 (ja) 2009-03-26

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PCT/JP2008/002574 WO2009037839A1 (ja) 2007-09-18 2008-09-18 細胞内に存在する被検物質の検出方法および検出キット

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JP (1) JPWO2009037839A1 (ja)
WO (1) WO2009037839A1 (ja)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2019078357A1 (ja) * 2017-10-20 2019-04-25 中外製薬株式会社 細胞への分子の取り込みを測定する方法
CN110646392A (zh) * 2019-09-30 2020-01-03 重庆大学 一种基于碳点的双发射比率荧光探针、制备方法及在多巴胺检测中的应用

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
DURSINA, BEATRICE ET AL.: "Identification and Specificity Profiling of Protein Prenyltransferase Inhibitors Using New Fluorescent Phosphoisoprenoids.", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, vol. 128, no. 9, 2006, pages 2822 - 2835 *
IMAI KAZUHIRO ET AL.: "Dynamic analytical chemistry: A trial study of the interaction of fluorogenic reagents with living chinese hamster ovary cells.", ANALYTICA CHIMICA ACTA, vol. 223, 1989, pages 299 - 308, XP026724704, DOI: doi:10.1016/S0003-2670(00)84095-7 *
PAGANO, RICHARD E. ET AL.: "Defining lipid transport pathways in animal cells.", SCIENCE, vol. 229, no. 4718, 1985, pages 1051 - 1057, XP002965545, DOI: doi:10.1126/science.4035344 *
TETSURO TAKAMATSU, KEIKO O MOCHIITA BIOIMAGING NO KISO - SEITAI LEVEL DENO KINO BUNSHI KAISEKI NI MUKETE -, WAKARU JIKKEN IGAKU SERIES BIOIMAGING GA WAKARU SAIBONAI BUNSHI O KANSATSU SURU TAYO NA GIJUTSU TO SONO GENRI, 2005, pages 10 - 19 *
WATANABE YOSHIHIKO ET AL.: "Pre- column labelling for high-performance liquid chromatography of amino acids with 7-fluoro- 4-nitrobenzo-2-oxa-1,3-diazole and its application to protein hydrolysates.", JOURNAL OF CHROMATOGRAPHY, vol. 239, 1982, pages 723 - 732, XP026538806, DOI: doi:10.1016/S0021-9673(00)82032-4 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2019078357A1 (ja) * 2017-10-20 2019-04-25 中外製薬株式会社 細胞への分子の取り込みを測定する方法
CN110646392A (zh) * 2019-09-30 2020-01-03 重庆大学 一种基于碳点的双发射比率荧光探针、制备方法及在多巴胺检测中的应用
CN110646392B (zh) * 2019-09-30 2020-11-03 重庆大学 碳点基双发射比率荧光探针在多巴胺检测中的应用

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JPWO2009037839A1 (ja) 2011-01-06

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