WO2009031228A1 - Method of detecting viral antigen and kit for detecting viral antigen - Google Patents

Method of detecting viral antigen and kit for detecting viral antigen Download PDF

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Publication number
WO2009031228A1
WO2009031228A1 PCT/JP2007/067419 JP2007067419W WO2009031228A1 WO 2009031228 A1 WO2009031228 A1 WO 2009031228A1 JP 2007067419 W JP2007067419 W JP 2007067419W WO 2009031228 A1 WO2009031228 A1 WO 2009031228A1
Authority
WO
WIPO (PCT)
Prior art keywords
antibody
viral antigen
detected
captured
detecting
Prior art date
Application number
PCT/JP2007/067419
Other languages
French (fr)
Japanese (ja)
Inventor
Reiko Tanaka
Original Assignee
Kuni Enterprise, Inc.
Tts Hien Company
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kuni Enterprise, Inc., Tts Hien Company filed Critical Kuni Enterprise, Inc.
Priority to PCT/JP2007/067419 priority Critical patent/WO2009031228A1/en
Priority to JP2009531156A priority patent/JPWO2009031351A1/en
Priority to PCT/JP2008/060870 priority patent/WO2009031351A1/en
Priority to TW097133413A priority patent/TW200933148A/en
Publication of WO2009031228A1 publication Critical patent/WO2009031228A1/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56983—Viruses

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Urology & Nephrology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Pathology (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

[PROBLEMS] To provide a method of detecting a viral antigen, whereby the viral antigen can be detected at a high accuracy while causing little false positive reaction even in the case of using a captured antibody and a detected antibody with nonhuman origins; and a kit for detecting a viral antigen. [MEANS FOR SOLVING PROBLEMS] A method of detecting a viral antigen contained in a human specimen, which contains an antibody against foreign immunoglobulin, comprising immobilizing a captured antibody with a nonhuman origin on a support and labeling a detected antibody with a nonhuman origin with a labeling agent. Next, the following steps are conducted either simultaneously or in this order, i.e., the step of adding an inhibitory antibody having the same epitope as an epitope, which is an epitope being common to the captured antibody and the detected antibody and recognized by the antibody against foreign immunoglobulin, to the human specimen and allowing it to bind to the antibody against foreign immunoglobulin; and the step of reacting the human specimen with the captured antibody and the detected antibody to thereby allow the captured antibody and the detected antibody to bind to the viral antigen. Subsequently, the labeling agent in the detected antibody bound to the viral antigen is detected. Since no bridge is formed between the captured antibody and the detected antibody, little false positive reaction arises.
PCT/JP2007/067419 2007-09-06 2007-09-06 Method of detecting viral antigen and kit for detecting viral antigen WO2009031228A1 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
PCT/JP2007/067419 WO2009031228A1 (en) 2007-09-06 2007-09-06 Method of detecting viral antigen and kit for detecting viral antigen
JP2009531156A JPWO2009031351A1 (en) 2007-09-06 2008-06-13 Viral antigen detection method and viral antigen detection kit
PCT/JP2008/060870 WO2009031351A1 (en) 2007-09-06 2008-06-13 Method of detecting viral antigen and kit for detecting viral antigen
TW097133413A TW200933148A (en) 2007-09-06 2008-09-01 Method of detecting viral antigen and kit for detecting viral antigen

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/JP2007/067419 WO2009031228A1 (en) 2007-09-06 2007-09-06 Method of detecting viral antigen and kit for detecting viral antigen

Publications (1)

Publication Number Publication Date
WO2009031228A1 true WO2009031228A1 (en) 2009-03-12

Family

ID=40428551

Family Applications (2)

Application Number Title Priority Date Filing Date
PCT/JP2007/067419 WO2009031228A1 (en) 2007-09-06 2007-09-06 Method of detecting viral antigen and kit for detecting viral antigen
PCT/JP2008/060870 WO2009031351A1 (en) 2007-09-06 2008-06-13 Method of detecting viral antigen and kit for detecting viral antigen

Family Applications After (1)

Application Number Title Priority Date Filing Date
PCT/JP2008/060870 WO2009031351A1 (en) 2007-09-06 2008-06-13 Method of detecting viral antigen and kit for detecting viral antigen

Country Status (3)

Country Link
JP (1) JPWO2009031351A1 (en)
TW (1) TW200933148A (en)
WO (2) WO2009031228A1 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115932269B (en) * 2022-12-26 2023-11-07 江阴天泽医学检验实验室有限公司 Method for carrying out antigen quantitative detection by adopting immunochromatography colloidal gold reagent

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6312960A (en) * 1986-07-04 1988-01-20 Green Cross Corp:The Aqueous solvent for immunological test
WO2006126287A1 (en) * 2005-05-27 2006-11-30 Masami Moriyama Method for detecting hiv-1 and kit to be used for the method

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6312960A (en) * 1986-07-04 1988-01-20 Green Cross Corp:The Aqueous solvent for immunological test
WO2006126287A1 (en) * 2005-05-27 2006-11-30 Masami Moriyama Method for detecting hiv-1 and kit to be used for the method

Also Published As

Publication number Publication date
JPWO2009031351A1 (en) 2011-01-27
WO2009031351A1 (en) 2009-03-12
TW200933148A (en) 2009-08-01

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