WO2009028421A1 - セリシンを用いたトランスフェクションデバイス - Google Patents

セリシンを用いたトランスフェクションデバイス Download PDF

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Publication number
WO2009028421A1
WO2009028421A1 PCT/JP2008/065027 JP2008065027W WO2009028421A1 WO 2009028421 A1 WO2009028421 A1 WO 2009028421A1 JP 2008065027 W JP2008065027 W JP 2008065027W WO 2009028421 A1 WO2009028421 A1 WO 2009028421A1
Authority
WO
WIPO (PCT)
Prior art keywords
sericin
device utilizing
transfection device
hydrolysate
utilizing sericin
Prior art date
Application number
PCT/JP2008/065027
Other languages
English (en)
French (fr)
Inventor
Yoshiji Fujita
Tomohiro Yoshikawa
Original Assignee
Cytopathfinder, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cytopathfinder, Inc. filed Critical Cytopathfinder, Inc.
Priority to CA2697658A priority Critical patent/CA2697658A1/en
Priority to EP08828531.7A priority patent/EP2194116B1/en
Priority to US12/674,836 priority patent/US20110165664A1/en
Priority to JP2009530086A priority patent/JP5172845B2/ja
Priority to AU2008292613A priority patent/AU2008292613B2/en
Publication of WO2009028421A1 publication Critical patent/WO2009028421A1/ja

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/87Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111General methods applicable to biologically active non-coding nucleic acids
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00Structure or type of the nucleic acid
    • C12N2310/10Type of nucleic acid
    • C12N2310/14Type of nucleic acid interfering N.A.
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2320/00Applications; Uses
    • C12N2320/30Special therapeutic applications
    • C12N2320/32Special delivery means, e.g. tissue-specific

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

 遺伝子試料、遺伝子デリバリー材料に加え、セリシン、その加水分解物又はそれらの化学修飾体からなる群から選択される少なくとも1種の化合物を含む混合物1を固相基材3に固着してあるトランスフェクションデバイス。
PCT/JP2008/065027 2007-08-24 2008-08-22 セリシンを用いたトランスフェクションデバイス WO2009028421A1 (ja)

Priority Applications (5)

Application Number Priority Date Filing Date Title
CA2697658A CA2697658A1 (en) 2007-08-24 2008-08-22 Transfection device utilizing sericin
EP08828531.7A EP2194116B1 (en) 2007-08-24 2008-08-22 Transfection device utilizing sericin hydrolysate
US12/674,836 US20110165664A1 (en) 2007-08-24 2008-08-22 Transfection device utilizing sericin
JP2009530086A JP5172845B2 (ja) 2007-08-24 2008-08-22 セリシンを用いたトランスフェクションデバイス
AU2008292613A AU2008292613B2 (en) 2007-08-24 2008-08-22 Transfection device utilizing sericin

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007-218733 2007-08-24
JP2007218733 2007-08-24

Publications (1)

Publication Number Publication Date
WO2009028421A1 true WO2009028421A1 (ja) 2009-03-05

Family

ID=40387143

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2008/065027 WO2009028421A1 (ja) 2007-08-24 2008-08-22 セリシンを用いたトランスフェクションデバイス

Country Status (6)

Country Link
US (1) US20110165664A1 (ja)
EP (1) EP2194116B1 (ja)
JP (1) JP5172845B2 (ja)
AU (1) AU2008292613B2 (ja)
CA (1) CA2697658A1 (ja)
WO (1) WO2009028421A1 (ja)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2015005431A1 (ja) 2013-07-12 2015-01-15 石原産業株式会社 細胞への遺伝子導入用組成物
CN112626013A (zh) * 2020-12-22 2021-04-09 哈尔滨赛信生物科技开发有限公司 一种无血清培养基及其应用

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10081816B1 (en) 2014-07-03 2018-09-25 Nant Holdings Ip, Llc Mechanical transfection devices and methods
US10760040B1 (en) 2014-07-03 2020-09-01 NanoCav, LLC Mechanical transfection devices and methods

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH11243948A (ja) * 1998-03-02 1999-09-14 Natl Inst Of Sericultural & Entomological Science 動物細胞増殖用の細胞培養床基材及びその調製方法
JP2003509060A (ja) * 1999-09-17 2003-03-11 ホワイトヘッド・インスティテュート・フォー・バイオメディカル・リサーチ 逆トランスフェクション方法
US6905878B2 (en) 2002-12-19 2005-06-14 The Scripps Research Institute DNA array for high throughput solid-phase transfection and method for producing the same

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH11243949A (ja) * 1998-03-03 1999-09-14 Toyobo Co Ltd Pqqを補欠分子族とするグルコースデヒドロゲナーゼおよびその製造方法
JP3256210B2 (ja) * 2000-01-18 2002-02-12 百樹 中川 繊維処理剤及び繊維の処理方法
US20040058407A1 (en) * 2001-04-10 2004-03-25 Miller Scott E. Reactor systems having a light-interacting component
JP4047176B2 (ja) * 2001-04-17 2008-02-13 セーレン株式会社 培地添加剤および動物細胞培養用培地
WO2002096978A1 (fr) * 2001-05-30 2002-12-05 Keiichi Miyamoto Elastine reticulee et son procede de production
US20090269825A1 (en) * 2005-11-11 2009-10-29 Toyo Boseki Kabushiki Kaisha Method for stabilization of biological molecule and composition

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH11243948A (ja) * 1998-03-02 1999-09-14 Natl Inst Of Sericultural & Entomological Science 動物細胞増殖用の細胞培養床基材及びその調製方法
JP2003509060A (ja) * 1999-09-17 2003-03-11 ホワイトヘッド・インスティテュート・フォー・バイオメディカル・リサーチ 逆トランスフェクション方法
US6544790B1 (en) 1999-09-17 2003-04-08 Whitehead Institute For Biomedical Research Reverse transfection method
US6905878B2 (en) 2002-12-19 2005-06-14 The Scripps Research Institute DNA array for high throughput solid-phase transfection and method for producing the same
JP2006511229A (ja) * 2002-12-19 2006-04-06 ザ スクリップス リサーチ インスティテュート 高処理能力の固相トランスフェクション用dnaアレイ及びその製造方法

Non-Patent Citations (14)

* Cited by examiner, † Cited by third party
Title
E.UCHIMURA ET AL.: "On-chip transfection of PC 12 cells based on the rational understanding of the role of ECM molecules: efficient, non-viral transfection ofPC12 cells using collagen IV", NEUROSCIENCE LETT., vol. 378, 2005, pages 40 - 43
HONMA K ET AL: "Atelocollagen-based gene transfer in cells allows high-throughput screening of gene functions", BIOCHEM. BIOPHYS. RES. COMMUN., vol. 289, 2001, pages 1075 - 1081, XP002957375 *
J.ZIAUDDIN; D.M.SABATINI: "Microarrays of cells expressed defined cDNAs", NATURE, vol. 411, 2001, pages 107 - 110
K.HONMA ET AL.: "Atelocollagen-based gene transfer in cells allows high-throughput screening of gene functions", BIOCHEM.BIOPHYS.RES.COMMU., vol. 289, 2001, pages 1075 - 1081
MOLECULAR CELL, vol. 12, 2003, pages 627 - 637
OCHIYA T ET AL: "New delivery system for plasmis DNA in vivo using atelocollagen as a carrier material", NAT. MED., vol. 5, 1999, pages 707 - 710, XP008131971 *
R.Z.WU ET AL.: "Cell-biological applications of transfected-cell microarrays", TRENDS IN CELL BIOLOGY, vol. 12, 2002, pages 485 - 488
See also references of EP2194116A4
T.OCHIYA ET AL.: "New delivery system for plasmid DNA in vivo using atelocollagen as a carrier material: the Minipellet", NATURE MED., vol. 5, 1998, pages 707 - 710
T.YOSHIKAWA ET AL.: "Transfection microarray of human mesenchymal stem cells and on-chip siRNA gene knockdown", J.CONTROLLED RELEASE, vol. 96, 2004, pages 227 - 232
UCHIMURA E ET AL: "On-chip transfection of PC12 cells based on the rational understanding of the role of eCM molecules: efficient, non-viral transfection of PC12 cells using collagen IV", NEUROSCI. LETT., vol. 378, 2005, pages 40 - 43, XP004778690 *
WU RZ ET AL: "Cell-biological applications of transfected-cell microarrays", TRENDS CELL BIOL., vol. 12, 2002, pages 485 - 488, XP001184158 *
YOSHIKAWA T ET AL: "Transfection microarray of human mesenchymal stem cells and on-chip siRNA gene knockdown", J. CONTROL RELEASE, vol. 96, 2004, pages 227 - 232, XP004502172 *
ZIAUDDIN J AND SABATINI DM: "Microarrays of cells expressing defined cDNAs", NATURE, vol. 411, 2001, pages 107 - 110, XP002963625 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2015005431A1 (ja) 2013-07-12 2015-01-15 石原産業株式会社 細胞への遺伝子導入用組成物
JPWO2015005431A1 (ja) * 2013-07-12 2017-03-02 石原産業株式会社 細胞への遺伝子導入用組成物
CN112626013A (zh) * 2020-12-22 2021-04-09 哈尔滨赛信生物科技开发有限公司 一种无血清培养基及其应用

Also Published As

Publication number Publication date
AU2008292613B2 (en) 2013-08-15
CA2697658A1 (en) 2009-03-05
EP2194116A4 (en) 2013-02-20
EP2194116A1 (en) 2010-06-09
US20110165664A1 (en) 2011-07-07
JP5172845B2 (ja) 2013-03-27
JPWO2009028421A1 (ja) 2010-12-02
AU2008292613A1 (en) 2009-03-05
EP2194116B1 (en) 2014-08-06

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