WO2009028294A1 - Mutant lipase and use thereof - Google Patents

Mutant lipase and use thereof Download PDF

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Publication number
WO2009028294A1
WO2009028294A1 PCT/JP2008/063748 JP2008063748W WO2009028294A1 WO 2009028294 A1 WO2009028294 A1 WO 2009028294A1 JP 2008063748 W JP2008063748 W JP 2008063748W WO 2009028294 A1 WO2009028294 A1 WO 2009028294A1
Authority
WO
WIPO (PCT)
Prior art keywords
lipase
produced
producing
substance
glycerol
Prior art date
Application number
PCT/JP2008/063748
Other languages
French (fr)
Japanese (ja)
Inventor
Yuya Kodama
Nobuhisa Shimba
Yoshihiro Usuda
Eiichiro Suzuki
Original Assignee
Ajinomoto Co., Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ajinomoto Co., Inc. filed Critical Ajinomoto Co., Inc.
Priority to BRPI0816114-3A2A priority Critical patent/BRPI0816114A2/en
Publication of WO2009028294A1 publication Critical patent/WO2009028294A1/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/32—Processes using, or culture media containing, lower alkanols, i.e. C1 to C6
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/08—Lysine; Diaminopimelic acid; Threonine; Valine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/22—Tryptophan; Tyrosine; Phenylalanine; 3,4-Dihydroxyphenylalanine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/18—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic polyhydric
    • C12P7/20—Glycerol

Abstract

Disclosed is a mutant lipase which is produced by introducing a mutation into a lipase produced by a microorganism belonging to the genus Cryptococcus, Gibberella, Ustilago or the like so that the enzymatic activity of the lipase is increased. The mutant lipase is allowed to act on an oil-and-fat, thereby producing glycerol. Glycerol thus produced is added to a culture medium as a carbon source, and a microorganism capable of producing a substance of interest (e.g., an amino acid) is cultured in the culture medium, thereby producing the substance.
PCT/JP2008/063748 2007-08-27 2008-07-31 Mutant lipase and use thereof WO2009028294A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
BRPI0816114-3A2A BRPI0816114A2 (en) 2007-08-27 2008-07-31 Mutant lipase, DNA, and, methods for producing a mutant lipase, glycerol, and a target substance.

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007220204 2007-08-27
JP2007-220204 2007-08-27

Publications (1)

Publication Number Publication Date
WO2009028294A1 true WO2009028294A1 (en) 2009-03-05

Family

ID=40387021

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2008/063748 WO2009028294A1 (en) 2007-08-27 2008-07-31 Mutant lipase and use thereof

Country Status (2)

Country Link
BR (1) BRPI0816114A2 (en)
WO (1) WO2009028294A1 (en)

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2004073123A (en) * 2002-08-20 2004-03-11 National Research Inst Of Brewing Lipase cs2 gene
JP2004075905A (en) * 2002-08-20 2004-03-11 National Research Inst Of Brewing Agent for decomposing biodegradable plastic and decomposing method
JP3507860B2 (en) * 2000-03-13 2004-03-15 独立行政法人酒類総合研究所 New yeast-derived lipase CS2
WO2006078039A1 (en) * 2005-01-18 2006-07-27 Ajinomoto Co., Inc. L-amino acid producing microorganism and a method for producing l-amino acid

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3507860B2 (en) * 2000-03-13 2004-03-15 独立行政法人酒類総合研究所 New yeast-derived lipase CS2
JP2004073123A (en) * 2002-08-20 2004-03-11 National Research Inst Of Brewing Lipase cs2 gene
JP2004075905A (en) * 2002-08-20 2004-03-11 National Research Inst Of Brewing Agent for decomposing biodegradable plastic and decomposing method
WO2006078039A1 (en) * 2005-01-18 2006-07-27 Ajinomoto Co., Inc. L-amino acid producing microorganism and a method for producing l-amino acid

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
KANIMI N.R. ET AL.: "Lipase catalyzed methanolysis of vegetable oils in aqueous medium by cryptococcus spp. S-2 process", BIOCHEMISTRY, vol. 37, no. 4, 2001, pages 405 - 410 *
KANIMI N.R. ET AL.: "Production,purification and characterization of an extracellular lipase from the yeast,cryptococcus sp. S-2 process", BIOCHEMISTRY, vol. 36, no. 4, 2000, pages 317 - 324 *
KAZUO MASAKI ET AL.: "Shinki koso ni yoru seibunkaisei plastic no bunkai", POLYMER PREPRINTS, vol. 55, no. 2, 2006, JAPAN, pages 5670 - 5671(3Z03) *
MASAKI K. ET AL.: "Cutinase-like enzyme from the yeast Cryptococcus sp.strain S-2 hydrolyzes polylactic acid and other biodegradeble plastics", APPL.ENVIRON.MICROBIOL., vol. 71, no. 11, 2005, pages 7548 - 7550 *

Also Published As

Publication number Publication date
BRPI0816114A2 (en) 2014-10-14

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