WO2008149807A1 - Es細胞の分化誘導方法 - Google Patents

Es細胞の分化誘導方法 Download PDF

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Publication number
WO2008149807A1
WO2008149807A1 PCT/JP2008/060029 JP2008060029W WO2008149807A1 WO 2008149807 A1 WO2008149807 A1 WO 2008149807A1 JP 2008060029 W JP2008060029 W JP 2008060029W WO 2008149807 A1 WO2008149807 A1 WO 2008149807A1
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cell
differentiation
induction
hepatocyte
culturing
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Ceased
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PCT/JP2008/060029
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English (en)
French (fr)
Inventor
Shoen Kume
Nobuaki Shiraki
Kahoko Umeda
Kazuhiko Kume
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Kumamoto University NUC
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Kumamoto University NUC
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Priority to EP08777040.0A priority Critical patent/EP2169051B1/en
Priority to JP2009517844A priority patent/JP5124820B2/ja
Priority to US12/602,120 priority patent/US8309353B2/en
Publication of WO2008149807A1 publication Critical patent/WO2008149807A1/ja
Anticipated expiration legal-status Critical
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/067—Hepatocytes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5073—Stem cells
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/12—Hepatocyte growth factor [HGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/16—Activin; Inhibin; Mullerian inhibiting substance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/237—Oncostatin M [OSM]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/38—Hormones with nuclear receptors
    • C12N2501/39—Steroid hormones
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    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/25—Urinary tract cells, renal cells
    • C12N2502/256—Renal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • Hematology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
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  • General Health & Medical Sciences (AREA)
  • Food Science & Technology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Toxicology (AREA)
  • General Engineering & Computer Science (AREA)
  • Developmental Biology & Embryology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

　本発明の課題は、ES細胞を確実に肝細胞に分化させるためのシステムを確立することで ある。本発明によれば、M15細胞の存在下で哺乳動物由来のES細胞を、アクチビン、及び bFGFの存在下で培養した後に、デキサメタゾン、HGF、及びオンコスタチンMの存在下で培 養することを含む、ES細胞から肝細胞へと分化誘導する方法が提供される。
PCT/JP2008/060029 2007-05-30 2008-05-30 Es細胞の分化誘導方法 Ceased WO2008149807A1 (ja)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP08777040.0A EP2169051B1 (en) 2007-05-30 2008-05-30 Method for induction of differentiation of es cell
JP2009517844A JP5124820B2 (ja) 2007-05-30 2008-05-30 Ｅｓ細胞の分化誘導方法
US12/602,120 US8309353B2 (en) 2007-05-30 2008-05-30 Method for induction of differentiation of ES cell

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007143225 2007-05-30
JP2007-143225 2007-05-30

Publications (1)

Publication Number Publication Date
WO2008149807A1 true WO2008149807A1 (ja) 2008-12-11

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ID=40093625

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PCT/JP2008/060029 Ceased WO2008149807A1 (ja) 2007-05-30 2008-05-30 Es細胞の分化誘導方法

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US (1) US8309353B2 (ja)
EP (1) EP2169051B1 (ja)
JP (1) JP5124820B2 (ja)
WO (1) WO2008149807A1 (ja)

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2010172225A (ja) * 2009-01-28 2010-08-12 Natl Inst Of Radiological Sciences 幹細胞におけるＴｅｘ１９遺伝子の発現量の変動に基づき該幹細胞での多能性又は分化能を判定又は検出する方法
JP2011115161A (ja) * 2009-11-05 2011-06-16 Kumamoto Univ 細胞の分化誘導方法
WO2012056997A1 (ja) 2010-10-28 2012-05-03 国立大学法人熊本大学 多能性幹細胞の分化誘導効率を改善するための方法及び培地
EP2457998A4 (en) * 2009-07-23 2013-08-21 Beijing Huayuanbochuang Technology Co Ltd METHODS FOR OBTAINING HEPATIC CELLS, HEPATIC ENDODERM CELLS, AND HEPATIC PRECURSOR CELLS BY INDUCING THE DIFFERENTIATION
JP2013538038A (ja) * 2010-08-13 2013-10-10 国立大学法人京都大学 多能性幹細胞から生殖細胞への分化誘導方法
WO2014199622A1 (ja) * 2013-06-10 2014-12-18 株式会社クラレ 組織構造体及びその作製方法
JP2015186471A (ja) * 2014-03-26 2015-10-29 ジーイー・ヘルスケア・ユーケイ・リミテッド 細胞分化方法
WO2018207789A1 (ja) * 2017-05-09 2018-11-15 国立大学法人京都大学 疾患ｉＰＳ細胞を用いた神経毒性評価モデル系及びその使用
WO2019208713A1 (ja) 2018-04-26 2019-10-31 国立大学法人東京工業大学 多能性幹細胞の分化促進方法

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9617517B2 (en) 2011-05-02 2017-04-11 National University Corporation Kumamoto University Small chemical compound which promotes induction of differentiation of stem cells into insulin-producing cells and method for inducing differentiation of stem cells into insulin-producing cells using said small chemical compound
US10023838B2 (en) 2013-03-01 2018-07-17 Kyoto University Method of inducing differentiation from pluripotent stem cells to germ cells
CA3175071A1 (en) 2020-03-11 2021-09-16 Bit Bio Limited Method of generating hepatic cells

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006126574A1 (ja) 2005-05-24 2006-11-30 Kumamoto University Es細胞の分化誘導方法

Patent Citations (1)

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Cited By (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2010172225A (ja) * 2009-01-28 2010-08-12 Natl Inst Of Radiological Sciences 幹細胞におけるＴｅｘ１９遺伝子の発現量の変動に基づき該幹細胞での多能性又は分化能を判定又は検出する方法
EP2457998A4 (en) * 2009-07-23 2013-08-21 Beijing Huayuanbochuang Technology Co Ltd METHODS FOR OBTAINING HEPATIC CELLS, HEPATIC ENDODERM CELLS, AND HEPATIC PRECURSOR CELLS BY INDUCING THE DIFFERENTIATION
JP2011115161A (ja) * 2009-11-05 2011-06-16 Kumamoto Univ 細胞の分化誘導方法
JP2013538038A (ja) * 2010-08-13 2013-10-10 国立大学法人京都大学 多能性幹細胞から生殖細胞への分化誘導方法
WO2012056997A1 (ja) 2010-10-28 2012-05-03 国立大学法人熊本大学 多能性幹細胞の分化誘導効率を改善するための方法及び培地
US10000739B2 (en) 2010-10-28 2018-06-19 National University Corporation Kumamoto University Method and culture medium for improving pluripotent stem cell differentiation inducing efficiency
JPWO2014199622A1 (ja) * 2013-06-10 2017-02-23 株式会社クラレ 組織構造体及びその作製方法
CN105283545A (zh) * 2013-06-10 2016-01-27 株式会社可乐丽 组织结构体及其制作方法
WO2014199622A1 (ja) * 2013-06-10 2014-12-18 株式会社クラレ 組織構造体及びその作製方法
CN105283545B (zh) * 2013-06-10 2019-12-10 株式会社可乐丽 组织结构体及其制作方法
US11060065B2 (en) 2013-06-10 2021-07-13 Corning Incorporated Tissue structure and preparation method thereof
JP2015186471A (ja) * 2014-03-26 2015-10-29 ジーイー・ヘルスケア・ユーケイ・リミテッド 細胞分化方法
WO2018207789A1 (ja) * 2017-05-09 2018-11-15 国立大学法人京都大学 疾患ｉＰＳ細胞を用いた神経毒性評価モデル系及びその使用
JPWO2018207789A1 (ja) * 2017-05-09 2020-03-12 国立大学法人京都大学 疾患ｉＰＳ細胞を用いた神経毒性評価モデル系及びその使用
JP7030343B2 (ja) 2017-05-09 2022-03-07 国立大学法人京都大学 疾患ｉＰＳ細胞を用いた神経毒性評価モデル系及びその使用
WO2019208713A1 (ja) 2018-04-26 2019-10-31 国立大学法人東京工業大学 多能性幹細胞の分化促進方法

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EP2169051A4 (en) 2010-09-01
JPWO2008149807A1 (ja) 2010-08-26
US8309353B2 (en) 2012-11-13
EP2169051B1 (en) 2016-02-10
EP2169051A1 (en) 2010-03-31
JP5124820B2 (ja) 2013-01-23
US20120058491A1 (en) 2012-03-08

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