WO2008144831A1 - Procédé et appareil d'inspection de tissu - Google Patents

Procédé et appareil d'inspection de tissu Download PDF

Info

Publication number
WO2008144831A1
WO2008144831A1 PCT/AU2008/000768 AU2008000768W WO2008144831A1 WO 2008144831 A1 WO2008144831 A1 WO 2008144831A1 AU 2008000768 W AU2008000768 W AU 2008000768W WO 2008144831 A1 WO2008144831 A1 WO 2008144831A1
Authority
WO
WIPO (PCT)
Prior art keywords
light
light transmitter
delivery
collection
additional
Prior art date
Application number
PCT/AU2008/000768
Other languages
English (en)
Inventor
Damian Keith Bird
Original Assignee
Invision Medical Technologies Pty Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Invision Medical Technologies Pty Ltd filed Critical Invision Medical Technologies Pty Ltd
Publication of WO2008144831A1 publication Critical patent/WO2008144831A1/fr

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00—Measuring for diagnostic purposes; Identification of persons
    • A61B5/68—Arrangements of detecting, measuring or recording means, e.g. sensors, in relation to patient
    • A61B5/6846—Arrangements of detecting, measuring or recording means, e.g. sensors, in relation to patient specially adapted to be brought in contact with an internal body part, i.e. invasive
    • A61B5/6847—Arrangements of detecting, measuring or recording means, e.g. sensors, in relation to patient specially adapted to be brought in contact with an internal body part, i.e. invasive mounted on an invasive device
    • A61B5/6848—Needles
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00—Measuring for diagnostic purposes; Identification of persons
    • A61B5/0059—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence
    • A61B5/0071—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence by measuring fluorescence emission
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00—Measuring for diagnostic purposes; Identification of persons
    • A61B5/0059—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence
    • A61B5/0082—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence adapted for particular medical purposes
    • A61B5/0084—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence adapted for particular medical purposes for introduction into the body, e.g. by catheters
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00—Measuring for diagnostic purposes; Identification of persons
    • A61B5/0059—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence
    • A61B5/0082—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence adapted for particular medical purposes
    • A61B5/0091—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence adapted for particular medical purposes for mammography
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00—Measuring for diagnostic purposes; Identification of persons
    • A61B5/43—Detecting, measuring or recording for evaluating the reproductive systems
    • A61B5/4306—Detecting, measuring or recording for evaluating the reproductive systems for evaluating the female reproductive systems, e.g. gynaecological evaluations
    • A61B5/4312—Breast evaluation or disorder diagnosis
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B23/00—Telescopes, e.g. binoculars; Periscopes; Instruments for viewing the inside of hollow bodies; Viewfinders; Optical aiming or sighting devices
    • G02B23/24—Instruments or systems for viewing the inside of hollow bodies, e.g. fibrescopes
    • G02B23/2407—Optical details
    • G02B23/2461—Illumination
    • G02B23/2469—Illumination using optical fibres
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B23/00—Telescopes, e.g. binoculars; Periscopes; Instruments for viewing the inside of hollow bodies; Viewfinders; Optical aiming or sighting devices
    • G02B23/24—Instruments or systems for viewing the inside of hollow bodies, e.g. fibrescopes
    • G02B23/26—Instruments or systems for viewing the inside of hollow bodies, e.g. fibrescopes using light guides
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B6/00—Light guides; Structural details of arrangements comprising light guides and other optical elements, e.g. couplings
    • G02B6/04—Light guides; Structural details of arrangements comprising light guides and other optical elements, e.g. couplings formed by bundles of fibres
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00—Measuring for diagnostic purposes; Identification of persons
    • A61B5/0059—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence
    • A61B5/0075—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence by spectroscopy, i.e. measuring spectra, e.g. Raman spectroscopy, infrared absorption spectroscopy
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B6/00—Light guides; Structural details of arrangements comprising light guides and other optical elements, e.g. couplings
    • G02B6/24—Coupling light guides
    • G02B6/26—Optical coupling means
    • G02B6/28—Optical coupling means having data bus means, i.e. plural waveguides interconnected and providing an inherently bidirectional system by mixing and splitting signals
    • G02B6/2804—Optical coupling means having data bus means, i.e. plural waveguides interconnected and providing an inherently bidirectional system by mixing and splitting signals forming multipart couplers without wavelength selective elements, e.g. "T" couplers, star couplers
    • G02B6/2808—Optical coupling means having data bus means, i.e. plural waveguides interconnected and providing an inherently bidirectional system by mixing and splitting signals forming multipart couplers without wavelength selective elements, e.g. "T" couplers, star couplers using a mixing element which evenly distributes an input signal over a number of outputs
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B6/00—Light guides; Structural details of arrangements comprising light guides and other optical elements, e.g. couplings
    • G02B6/24—Coupling light guides
    • G02B6/26—Optical coupling means
    • G02B6/32—Optical coupling means having lens focusing means positioned between opposed fibre ends

Definitions

  • the present invention relates to a method and apparatus for inspecting tissue samples, such as in or from suspicious breast masses, and in particular to a fibre optic device for multiphoton excitation of fluorescence and collection of emitted fluorescence at a lesion site for indication of disease therein by time- resolved fluorescence measurements, such as prior to tissue biopsy.
  • a typical rigid endoscope can contain as many as 60 lens components for relaying the image from the inside of the body. Diameters of optical components can be 1 mm or less. Quantities sold by any one manufacturer range only between a few hundred and a few thousand endoscopes per year but production techniques have always contained an element of craft. Accelerating interest in the last decade in advanced endoscopes with superior brightness and resolution and smaller diameter have advanced the limits of technology in producing these lens systems cost- effectively. Most medical endoscopes are manufactured in Germany or Japan. Selfoc gradient-index rod lenses, which are manufactured primarily for table-top copiers, have proved to be important in the development of small diameter endoscopes.
  • FLIM time-resolved fluorescence analysis
  • FLIM fluorescence lifetime imaging'
  • FLIM provides an additional data dimension with opportunities for functional imaging and is a direct approach to all effects that involve energy transfer.
  • Typical examples are the mapping of cell parameters such as pH, ion concentrations or oxygen saturation by fluorescence quenching, and fluorescence resonance energy transfer between different fluorophores in the cell [4].
  • the technique also allows the difficulties associated with different fluorescent biomarkers in multi- stained samples (or with different natural fluorophores within the cells themselves) to be overcome by clearly distinguishing individual emitting species via their characteristic fluorescence lifetime.
  • the invention provides a method and apparatus for inspecting tissue.
  • the invention provides an apparatus for inspecting tissue, comprising: a light source of coherent light; a needle with a bore and a tip; a delivery light transmitter (typically comprising one — but possibly more — optical fibres) for transmitting light from the light source to an exit tip of the delivery light transmitter; a collection light transmitter (typically comprising one or more optical fibres) for collecting return light; and a photodetector; wherein a portion of the delivery light transmitter is located in the bore so that the exit tip can be introduced into a sample by inserting the tip of the needle into the sample and illuminating a volume of the sample with light from the light source, and a portion of the collection light transmitter is located in the bore to collect return light from the sample and transmit it to the photodetector.
  • the apparatus may comprise an additional delivery light transmitter for transmitting light from the light source to an exit tip of the additional delivery light transmitter, and an additional collection light transmitter for collecting return light, wherein a portion of the additional delivery light transmitter is located in the bore so that the exit tip of the additional delivery light transmitter is introduced into the sample with the exit tip of the delivery light transmitter, to illuminate an additional volume of the sample with light from the light source, and a portion of the additional collection light transmitter is located in the bore to collect return light from the sample and transmit it to the photodetector.
  • the light source may comprise a plurality of individual light sources (such as one for each delivery light transmitter). Also, still further delivery light transmitters and collection light transmitters may be provided, so that multiple volumes of the sample can be illuminated.
  • the apparatus may comprise an additional needle with a respective bore and tip, an additional delivery light transmitter for transmitting light from the light source to an exit tip of the additional delivery light transmitter, and an additional collection light transmitter for collecting return light, wherein a portion of the additional delivery light transmitter is located in the bore of the additional needle so that the exit tip of the additional delivery light transmitter can be introduced into a sample by inserting the tip of the additional needle into the sample and illuminating a volume of the sample with light from the light source, and a portion of the additional collection light transmitter is located in the bore of the additional needle to collect return light from the sample and transmit it to the photodetector.
  • the invention provides a method for inspecting tissue, comprising employing the apparatus described above to inspect and characterize a sample.
  • the method comprises: inserting a tip of a needle into a sample, the needle having a bore containing a portion of a (typically flexible) delivery light transmitter and a portion of a (typically flexible) collection light transmitter; transmitting light with the delivery light transmitter from a light source of coherent light to an exit tip of the delivery light transmitter to illuminate a volume of the sample with the light; collecting return light with the collection light transmitter; and transmitting the collected return light with the collection light transmitter to a photodetector.
  • the invention provides a device for use in a system for inspecting tissue, comprising: a needle with a bore and a tip; a delivery light transmitter optically couplable (such as with a flexible light transmitter) to a light source, for transmitting light from the light source to an exit tip of the delivery light transmitter; and a collection light transmitter optically couplable (such as with a flexible light transmitter) to a photodetector, for collecting return light; wherein the delivery light transmitter and the collection light transmitter are located in the bore so that the exit tip can be introduced into a sample by inserting the tip of the needle into the sample and illuminating a volume of the sample with light from the light source, and the collection light transmitter is located in the bore to collect return light from the sample and transmit the collected return light to the photodetector.
  • Figure 1 is a schematic diagram of an optical biopsy system according to an embodiment of the present invention.
  • Figure 2 is a schematic view of a detail of figure 1, showing the needle and a portion of the fibres of the system of figure 1 ;
  • Figure 4 is a schematic view of the exit ends of the collection fibres and photodetectors of the system of figure 1;
  • Figure 5 is a schematic diagram of an optical biopsy system according to another embodiment of the present invention.
  • Figure 6 is a schematic diagram of an optical biopsy system according to yet another embodiment of the present invention.
  • Figure 7 is a schematic diagram of an optical biopsy system according to a further embodiment of the present invention.
  • Figure 8A is a cross sectional view of the needle and enclosed fibres of the system of figure 7;
  • Figure 8B is a cross sectional view of an alternative arrangement of the needle and enclosed fibres of the system of figure 7; and Figure 8C is a cross sectional view of an alternative needle (with enclosed fibres) of the system of figure 7.
  • FIG. 1 is a schematic diagram of an optical biopsy system 10 according to an embodiment of the present invention.
  • the system 10 includes a pulsed coherent light source in the form of a femtosecond pulsed laser source 12 for providing illuminating light (which may be a single photon source, but in this embodiment is a two-photon source), neutral density filter 14 and a focussing lens 16 for focussing the illuminating light output by laser source 12.
  • a pulsed coherent light source in the form of a femtosecond pulsed laser source 12 for providing illuminating light (which may be a single photon source, but in this embodiment is a two-photon source)
  • neutral density filter 14 which may be a single photon source, but in this embodiment is a two-photon source
  • a focussing lens 16 for focussing the illuminating light output by laser source 12.
  • System 10 is arranged for performing two-photon excitation but, in other embodiments, single-photon excitation may be employed.
  • single-photon excitation may be employed.
  • certain pulsed laser diode sources that are capable of single photon excitation only may be employed.
  • Laser source 12 emits light in the approximate range of 700 to 900 nm at a pulse repetition rate in the approximate range of 75 to 82 MHz.
  • the neutral density filter 14 may be rotated to vary the power of illuminating light to be employed.
  • the system 10 further includes a low dispersion delivery optical fibre in the form of a zero-dispersion wavelength delivery photonic crystal fibre (PCF) 18 for receiving the illuminating light from focussing lens 16 and transmitting it to a sample located at its distal or exit end 20, a focussing element (not shown) attached to the delivery PCF 18 at its exit end 20 for focussing the illuminating light onto or into the sample and six high numerical aperture multimode collection fibres 22 (which may be standard multimode fibres but, in this embodiment, are PCFs) for collecting return fluorescent light from the sample.
  • PCF photonic crystal fibre
  • the focussing element may comprise, for example, a single lens, multiple lenses, or an exit portion of the delivery PCF 18 (which may be, for example, convex).
  • the delivery PCF 18 which may be, for example, convex.
  • it comprises one or more microlenses, such as gradient index rod lenses of numerical aperture matching closely (if not exactly) that of the delivery PCF 18, affixed rigidly to the delivery PCF 18 with a fibre optic resin of appropriate refractive index.
  • the system 10 also includes a conventional biopsy needle 24 (such as a 16G needle) in which the delivery PCF 18 and collection PCFs 22 are located for insertion into the sample.
  • a conventional biopsy needle 24 such as a 16G needle
  • the delivery PCF 18 and collection PCFs 22 are located for insertion into the sample.
  • needle 24 the portions of delivery PCF 18, collection PCFs 22 enclosed within needle 24 and the focussing element constitute a fibre probe. Insertion of the needle 24 into the sample inserts the bundle 26 of delivery PCF 18 and collection PCFs 22.
  • FIG 2 is a schematic, enlarged view 28 of needle 24 and delivery PCF 18 and collection PCFs 22.
  • Figure 3 is a cross section view 30 of the needle 24 and its contents: delivery PCF 18 and collection PCFs 22. It will be seen that the delivery PCF 18 is located within the six collection PCFs 22, which are hence closely packed.
  • Emitted two-photon excited fluorescence is collected by the collection PCFs 22 (at their entry ends adjacent to and around the exit end 20 of delivery PCF 18). As is apparent from figure 3, the centre-to-centre spacing between adjacent collection PCFs 22 is the diameter d of an individual fibre. Collected fluorescence traverses the collection PCFs 22
  • system 10 includes a fibre chuck 32 at the end of the collection PCFs 22 (and, in some embodiments, a positioning device for adjusting the position of the fibre chuck).
  • System 10 also includes one or more barrier filters 34 optically after the collection PCFs 22, for removing residual fundamental illumination, and then a photodetector 36 in the form of one or more photomultiplier tubes coupled to photon counting electronics 38 for counting or otherwise determining a measure of the number of returned photons.
  • system 10 includes six photomultiplier tubes (or other photodetectors), one for each of collection PCFs 22.
  • photodetector 36 comprises one or more single photon avalanche diodes (SPAD) detectors and a time-correlated single photon counting module.
  • SPAD single photon avalanche diodes
  • This arrangement is particularly adapted to providing sensitive detection of endogenous fluorescence lifetime signals in, for example, breast cancer lesions.
  • system 10 does not employ an optical coupler, but rather comprises an arrangement of individual fibres (delivery PCF 18 and collection PCFs 22).
  • Other embodiments may include a single mode 2 x 1 optical fibre coupler or a 2 x 1 (preferably double clad) PCF coupler, located at the point where the delivery PCF 18 and collection PCFs 22 meet in system 10.
  • needle 24 may contain only a single PCF for both delivering light to and collecting light from the sample, which would allow a needle with a smaller bore to be used.
  • a double clad PCF is provided from the coupler to the tip of the needle 24. A portion of the collected light is then directed by the coupler to the photodetector 36 upon its return from the sample.
  • additional returned fluorescence which enters the exit end 20 of delivery PCF 18, may be collected from delivery PCF 18 — such as with a beamsplitter, coupler or otherwise — and combined with that collected by collection PCFs 22 and passed to photodetector 36 (in some embodiments comprising one or more photomultiplier tubes or SPAD detectors), via barrier filter 34.
  • photodetector 36 in some embodiments comprising one or more photomultiplier tubes or SPAD detectors
  • Figure 4 is a schematic view of the arrangement 40 of the exit ends 42 of collections PCFs 22 (i.e. as held by fibre chuck 32).
  • the exit ends 42 are arranged linearly for convenient delivery of collected light 44 to one or more photodetectors, and in this embodiment an array 46 of six photodetectors or spectrometers 37 (collectively constituting photodetector 36).
  • barrier filter 34 may itself comprise a plurality of separate portions 35 of filter material, according to desired geometry or other constraints.
  • the collection PCFs 22 are spaced either by of (the diameter of each PCF 22), V3 d (i.e. two collection PCFs 22 separated by a third) or 2d (i.e. two collection PCFs 22 on opposite sides of delivery PCF 18).
  • V3 d i.e. two collection PCFs 22 separated by a third
  • 2d i.e. two collection PCFs 22 on opposite sides of delivery PCF 18.
  • this arrangement facilitates the positioning of the fibre probe (comprising needle 24 and enclosed portions of delivery and collection PCFs 18, 22), and hence a biopsy excision device introduced along the track of needle 24, closer to a suspicious site.
  • a first of collection PCFs 22 detects a time-domain signal indicative of suspect tissue but that signal is not apparent in the light collected from a second of collection PCFs 22 at a distance V3 d or 2dfrom the first, the operator could move the biopsy excision device along the track of needle 24 but in the direction of the first of these collection PCFs 22 and hence, in all likelihood, closer to the lesion.
  • the invention provides an optical biopsy system 50 that is similar to system 10 (and like references numerals have been used to indicate like features), but with a coherent light source in the form of a fibre laser 52. (Consequently, neutral density filter 14 and a focussing lens 16 of system 10 are omitted).
  • Fibre laser 52 allows a less expensive and more compact system to be provided.
  • Ultrashort-pulsed fibre lasers capable of producing sub 500 femtosecond pulses have been reported [17, 18] (though this is usually achieved through soliton generation around the 1.5 ⁇ m wavelength).
  • Efficient frequency doubling techniques have been implemented that provide a more suitable operating wavelength of 768 nm for two-photon excitation [19]. Such an arrangement may have comparatively low peak power, but may be suitable for some applications.
  • a fibre bundle based collection system is provided.
  • Fibre bundle based systems have been proposed [20, 21 , 22, 24] for in vivo applications as, in combination with galvanometric scanning mirrors, it is possible to scan the proximal end of a bundle of single-mode fibres to reconstruct an image from the detected signal at the distal end.
  • the practical advantage comes at the expense of axial resolution [23] since, in general, fibre-bundle based imaging systems have a comparatively poor optical sectioning ability.
  • a hybrid detection system that provides the simultaneous operation of time-resolved multiphoton fluorescence endomicroscopy and another imaging modality.
  • One potential imaging modality that would be suitable for such a hybrid is second harmonic generation (SHG).
  • SHG second harmonic generation
  • the tip of biopsy needle 24 can be located in a tissue to be screened for abnormalities, and the collected data (indicative of the collected return light) compared with a library of comparable data from tissue known to have been normal.
  • the tip of biopsy needle 24 can be located successively in comparable tissue at different sites (possibly in different subjects) where at least one of those sites is thought to be healthy.
  • FIG. 6 is a schematic diagram of an optical biopsy system 60 according to an embodiment of the present invention.
  • System 60 essentially comprises system 10 (and like references numerals have been used to indicate like features), but with the addition of a second low dispersion delivery optical fibre 18', a second biopsy needle 24', and a second set of multimode collection fibres 22' (which may be standard multimode fibres but, in this embodiment, are PCFs).
  • Fibre chuck 32 thus — holds the exit ends of first and second sets of collection PCFs 22, 22', and photodetector 36 comprises an array of twelve photodetectors or spectrometers each corresponding to one of the collection fibres. The output of photodetector 36 is again input into photon counting electronics 38.
  • System 60 includes a optical coupler 62 for use as a beamsplitter, located optically after focussing lens 16. (Other beamsplitters may alternatively be employed, such as a right-angle partially reflecting prism.) Coupler 62 splits the light from pulsed laser source 12 equally into its two downstream arms, which are coupled respectively to (first) delivery optical fibre 18 and second delivery optical fibre 18'.
  • first and second delivery optical fibres 18, 18' and first and second sets of collection PCFs 22, 22' are provided (cf. system 60 of figure 6), but with a single biopsy needle.
  • Figure 7 is a schematic diagram of an optical biopsy system 70 according to an embodiment of the present invention.
  • System 70 essentially comprises many of the features of system 60 and like references numerals have been used to indicate like features.
  • System 70 thus has an optical coupler 62 for splitting the light from pulsed laser source 12 equally into its two downstream arms, which are coupled respectively to first delivery optical fibre 18 and second delivery optical fibre 18'.
  • the fibre bundle 72 comprising first delivery optical fibre 18, second delivery optical fibre 18', first collection PCFs 22 and second collection PCFs 22' are inserted into a single biopsy needle 74.
  • Figure 8A is a cross section view 80 of needle 74 and its contents: first delivery PCF 18, second delivery PCF 18', first collection multimode fibres 22 PCFs 22 and second collection multimode fibres 22'.
  • first collection fibres 22 and second collection fibres 22' are all PCFs, but in other embodiments may be standard multimode fibres.
  • first delivery PCF 18 is located within the first six collection PCFs 22, which are hence closely packed, and that second delivery PCF 18' is located within the second six collection PCFs 22', which are also closely packed.
  • first delivery PCF 18 illuminates a respective volume of the sample, and emitted two-photon excited fluorescence is collected by the respective collection PCFs 22,22' (at their entry ends adjacent to and around the respective exit ends of delivery PCFs 18,18').
  • the centre-to-centre spacing between adjacent collection PCFs 22,22' is the diameter d of an individual fibre.
  • the respective illuminated volumes have centres separated by 3d.
  • this separation can be increased — according to other embodiments — as desired, to increase collection efficiency.
  • one or more separating members can be located between the respective collection PCFs 22,22', such as in the form of one or more additional fibres.
  • Figure 8B is a cross section view 90 of an alternative needle 92 and its contents; needle 92 is comparable to needle 74 of figure 8A, and is also for use with system 70 of figure 7.
  • an intermediate, spacing fibre 94 is located between the respective collection PCFs 22,22' to increase the separate of delivery PCFs 18,18' and hence of the illuminated volumes.
  • needle 92 may, in some embodiments, contain a portion of a fibre bundle of which seven adjacent fibres - generally located at or near the wall of needle 92 - constitute first delivery PCF 18 and first collection PCFs 22, and another seven adjacent fibres - generally located at or near a diametrically opposite wall of needle 92 - constitute second delivery PCF 18' and second collection PCFs 22'.
  • Other fibres in the bundle can either merely serve to securely locate the delivery and collection fibres, or - in some embodiments - constitute further sets of delivery and collection fibres.
  • a needle for use with system 70 of figure 7, a needle is provided with two bores, each for receiving a respective bundle of delivery and collection fibres.
  • Figure 8C is a cross section view 100 of an alternative needle 102 (for use with system 70 of figure 7) and its contents. Needle 102 has two bores 104, 104'; the first bore 104 encloses first delivery PCF 18 and first collection PCFs 22, while the second bore 104' encloses second delivery PCF 18' and second collection PCFs 22'.
  • the needle may be provided with additional bores, each enclosing respective delivery and collection fibres.
  • collection PCFs 22, 22' may collect light from both volumes excited by delivery PCFs 18,18'.
  • the diagnostic signal may be said to be mixed.
  • delivery PCF 18 illuminates a volume at the boundary between normal and malignant tissue
  • delivery PCF 18' illuminates only normal tissue
  • the user would be prompted to move needle 74, 92 towards the malignant tissue until the signals more closely matched or were indicative of malignant tissue in all collection fibres (particularly those collection fibres between delivery PCFs 18, 18').
  • the user would have located a suitable and possibly optimal location to take a biopsy, as he or she could have greater confidence that the biopsy would be made into malignant tissue.

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Molecular Biology (AREA)
  • Veterinary Medicine (AREA)
  • Biophysics (AREA)
  • Pathology (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Public Health (AREA)
  • Medical Informatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Surgery (AREA)
  • Animal Behavior & Ethology (AREA)
  • Optics & Photonics (AREA)
  • General Physics & Mathematics (AREA)
  • Astronomy & Astrophysics (AREA)
  • Gynecology & Obstetrics (AREA)
  • Reproductive Health (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

L'invention concerne un appareil d'inspection de tissu, comprenant une source lumineuse ayant une lumière cohérente, une aiguille avec un alésage et une pointe, un émetteur de lumière de distribution pour émettre une lumière depuis la source lumineuse vers une pointe de sortie de l'émetteur de lumière de distribution, un émetteur de lumière de collecte pour collecter une lumière de retour, et un photodétecteur. Une partie de l'émetteur de lumière de distribution est située dans l'alésage de telle sorte que la pointe de sortie peut être introduite dans un échantillon par introduction de la pointe de l'aiguille dans l'échantillon et par éclairage d'un volume de l'échantillon avec la lumière provenant de la source lumineuse, et une partie de l'émetteur de lumière de collecte est située dans l'alésage pour collecter la lumière de retour provenant de l'échantillon et transmettre la lumière de retour collectée au photodétecteur.
PCT/AU2008/000768 2007-05-30 2008-05-30 Procédé et appareil d'inspection de tissu WO2008144831A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US94093307P 2007-05-30 2007-05-30
US60/940,933 2007-05-30

Publications (1)

Publication Number Publication Date
WO2008144831A1 true WO2008144831A1 (fr) 2008-12-04

Family

ID=40074464

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/AU2008/000768 WO2008144831A1 (fr) 2007-05-30 2008-05-30 Procédé et appareil d'inspection de tissu

Country Status (1)

Country Link
WO (1) WO2008144831A1 (fr)

Cited By (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8385695B2 (en) 2009-11-23 2013-02-26 Corning Incorporated Optical fiber imaging system and method for generating fluorescence imaging
WO2015005999A1 (fr) * 2013-07-09 2015-01-15 Siemens Energy, Inc. Système et procédé d'acquisition d'images par fibre optique convenant à une utilisation dans des moteurs à turbine
WO2015113113A1 (fr) * 2014-02-03 2015-08-06 The University Of Western Australia Dispositif médical à introduire dans une matière pour obtenir un échantillon de matière, et son procédé
US10158038B1 (en) 2018-05-17 2018-12-18 Hi Llc Fast-gated photodetector architectures comprising dual voltage sources with a switch configuration
US10340408B1 (en) 2018-05-17 2019-07-02 Hi Llc Non-invasive wearable brain interface systems including a headgear and a plurality of self-contained photodetector units configured to removably attach to the headgear
US10515993B2 (en) 2018-05-17 2019-12-24 Hi Llc Stacked photodetector assemblies
US10868207B1 (en) 2019-06-06 2020-12-15 Hi Llc Photodetector systems with low-power time-to-digital converter architectures to determine an arrival time of photon at a photodetector based on event detection time window
US11006876B2 (en) 2018-12-21 2021-05-18 Hi Llc Biofeedback for awareness and modulation of mental state using a non-invasive brain interface system and method
US11081611B2 (en) 2019-05-21 2021-08-03 Hi Llc Photodetector architectures for efficient fast-gating comprising a control system controlling a current drawn by an array of photodetectors with a single photon avalanche diode
US11096620B1 (en) 2020-02-21 2021-08-24 Hi Llc Wearable module assemblies for an optical measurement system
US11187575B2 (en) 2020-03-20 2021-11-30 Hi Llc High density optical measurement systems with minimal number of light sources
US11213245B2 (en) 2018-06-20 2022-01-04 Hi Llc Spatial and temporal-based diffusive correlation spectroscopy systems and methods
US11213206B2 (en) 2018-07-17 2022-01-04 Hi Llc Non-invasive measurement systems with single-photon counting camera
US11245404B2 (en) 2020-03-20 2022-02-08 Hi Llc Phase lock loop circuit based signal generation in an optical measurement system
US11515014B2 (en) 2020-02-21 2022-11-29 Hi Llc Methods and systems for initiating and conducting a customized computer-enabled brain research study
US11607132B2 (en) 2020-03-20 2023-03-21 Hi Llc Temporal resolution control for temporal point spread function generation in an optical measurement system
US11630310B2 (en) 2020-02-21 2023-04-18 Hi Llc Wearable devices and wearable assemblies with adjustable positioning for use in an optical measurement system
US11645483B2 (en) 2020-03-20 2023-05-09 Hi Llc Phase lock loop circuit based adjustment of a measurement time window in an optical measurement system
US11771362B2 (en) 2020-02-21 2023-10-03 Hi Llc Integrated detector assemblies for a wearable module of an optical measurement system
US11813041B2 (en) 2019-05-06 2023-11-14 Hi Llc Photodetector architectures for time-correlated single photon counting
US11819311B2 (en) 2020-03-20 2023-11-21 Hi Llc Maintaining consistent photodetector sensitivity in an optical measurement system
US11857348B2 (en) 2020-03-20 2024-01-02 Hi Llc Techniques for determining a timing uncertainty of a component of an optical measurement system
US11864867B2 (en) 2020-03-20 2024-01-09 Hi Llc Control circuit for a light source in an optical measurement system by applying voltage with a first polarity to start an emission of a light pulse and applying voltage with a second polarity to stop the emission of the light pulse
US11877825B2 (en) 2020-03-20 2024-01-23 Hi Llc Device enumeration in an optical measurement system
US11883181B2 (en) 2020-02-21 2024-01-30 Hi Llc Multimodal wearable measurement systems and methods
US11903676B2 (en) 2020-03-20 2024-02-20 Hi Llc Photodetector calibration of an optical measurement system
US11950879B2 (en) 2020-02-21 2024-04-09 Hi Llc Estimation of source-detector separation in an optical measurement system
US11969259B2 (en) 2020-02-21 2024-04-30 Hi Llc Detector assemblies for a wearable module of an optical measurement system and including spring-loaded light-receiving members
US12029558B2 (en) 2020-02-21 2024-07-09 Hi Llc Time domain-based optical measurement systems and methods configured to measure absolute properties of tissue
US12059262B2 (en) 2020-03-20 2024-08-13 Hi Llc Maintaining consistent photodetector sensitivity in an optical measurement system
US12059270B2 (en) 2020-04-24 2024-08-13 Hi Llc Systems and methods for noise removal in an optical measurement system
US12085789B2 (en) 2020-03-20 2024-09-10 Hi Llc Bias voltage generation in an optical measurement system

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001059423A2 (fr) * 2000-02-08 2001-08-16 Cornell Research Foundation, Inc. Excitation multiphotonique par fibres optiques pour spectroscopie par fluorescence
US20010055462A1 (en) * 2000-06-19 2001-12-27 Seibel Eric J. Medical imaging, diagnosis, and therapy using a scanning single optical fiber system
AU2003204717A1 (en) * 2002-09-30 2004-04-22 Swinburne University Of Technology Apparatus
WO2005031435A1 (fr) * 2003-09-22 2005-04-07 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Micro-endoscope confocal comprenant des fibres optiques a diametre decroissant
WO2005082225A1 (fr) * 2004-02-27 2005-09-09 Optiscan Pty Ltd Element optique
WO2006076772A1 (fr) * 2005-01-21 2006-07-27 Optiscan Pty Ltd Endomicroscope à foyer commun avec faisceau de fibres
WO2006076759A1 (fr) * 2005-01-21 2006-07-27 Optiscan Pty Ltd Faisceau de fibres pour endomicroscopie de contact

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001059423A2 (fr) * 2000-02-08 2001-08-16 Cornell Research Foundation, Inc. Excitation multiphotonique par fibres optiques pour spectroscopie par fluorescence
US20010055462A1 (en) * 2000-06-19 2001-12-27 Seibel Eric J. Medical imaging, diagnosis, and therapy using a scanning single optical fiber system
AU2003204717A1 (en) * 2002-09-30 2004-04-22 Swinburne University Of Technology Apparatus
WO2005031435A1 (fr) * 2003-09-22 2005-04-07 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Micro-endoscope confocal comprenant des fibres optiques a diametre decroissant
WO2005082225A1 (fr) * 2004-02-27 2005-09-09 Optiscan Pty Ltd Element optique
WO2006076772A1 (fr) * 2005-01-21 2006-07-27 Optiscan Pty Ltd Endomicroscope à foyer commun avec faisceau de fibres
WO2006076759A1 (fr) * 2005-01-21 2006-07-27 Optiscan Pty Ltd Faisceau de fibres pour endomicroscopie de contact

Cited By (44)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8385695B2 (en) 2009-11-23 2013-02-26 Corning Incorporated Optical fiber imaging system and method for generating fluorescence imaging
WO2015005999A1 (fr) * 2013-07-09 2015-01-15 Siemens Energy, Inc. Système et procédé d'acquisition d'images par fibre optique convenant à une utilisation dans des moteurs à turbine
CN105378539A (zh) * 2013-07-09 2016-03-02 西门子能源公司 适合于在涡轮发动机中使用的用于基于光纤的图像获取的系统和方法
US9654741B2 (en) 2013-07-09 2017-05-16 Siemens Energy, Inc. System and method for optical fiber based image acquisition suitable for use in turbine engines
DE112014003206B4 (de) 2013-07-09 2023-03-02 Siemens Energy, Inc. System und Verfahren zur glasfaserbasierten Bilderfassung mit Eignung für den Einsatz in Turbinenmotoren
WO2015113113A1 (fr) * 2014-02-03 2015-08-06 The University Of Western Australia Dispositif médical à introduire dans une matière pour obtenir un échantillon de matière, et son procédé
US10052086B2 (en) 2014-02-03 2018-08-21 The University Of Western Australia Medical device for insertion into a material to obtain a material sample and a method thereof
US10672936B2 (en) 2018-05-17 2020-06-02 Hi Llc Wearable systems with fast-gated photodetector architectures having a single photon avalanche diode and capacitor
US11004998B2 (en) 2018-05-17 2021-05-11 Hi Llc Wearable brain interface systems including a headgear and a plurality of photodetector units
US10515993B2 (en) 2018-05-17 2019-12-24 Hi Llc Stacked photodetector assemblies
US10340408B1 (en) 2018-05-17 2019-07-02 Hi Llc Non-invasive wearable brain interface systems including a headgear and a plurality of self-contained photodetector units configured to removably attach to the headgear
US10672935B2 (en) 2018-05-17 2020-06-02 Hi Llc Non-invasive wearable brain interface systems including a headgear and a plurality of self-contained photodetector units
US10847563B2 (en) 2018-05-17 2020-11-24 Hi Llc Wearable systems with stacked photodetector assemblies
US11437538B2 (en) 2018-05-17 2022-09-06 Hi Llc Wearable brain interface systems including a headgear and a plurality of photodetector units each housing a photodetector configured to be controlled by a master control unit
US10424683B1 (en) 2018-05-17 2019-09-24 Hi Llc Photodetector comprising a single photon avalanche diode and a capacitor
US10158038B1 (en) 2018-05-17 2018-12-18 Hi Llc Fast-gated photodetector architectures comprising dual voltage sources with a switch configuration
US11213245B2 (en) 2018-06-20 2022-01-04 Hi Llc Spatial and temporal-based diffusive correlation spectroscopy systems and methods
US11213206B2 (en) 2018-07-17 2022-01-04 Hi Llc Non-invasive measurement systems with single-photon counting camera
US11006876B2 (en) 2018-12-21 2021-05-18 Hi Llc Biofeedback for awareness and modulation of mental state using a non-invasive brain interface system and method
US11903713B2 (en) 2018-12-21 2024-02-20 Hi Llc Biofeedback for awareness and modulation of mental state using a non-invasive brain interface system and method
US11813041B2 (en) 2019-05-06 2023-11-14 Hi Llc Photodetector architectures for time-correlated single photon counting
US11081611B2 (en) 2019-05-21 2021-08-03 Hi Llc Photodetector architectures for efficient fast-gating comprising a control system controlling a current drawn by an array of photodetectors with a single photon avalanche diode
US11398578B2 (en) 2019-06-06 2022-07-26 Hi Llc Photodetector systems with low-power time-to-digital converter architectures to determine an arrival time of photon at a photodetector based on event detection time window
US10868207B1 (en) 2019-06-06 2020-12-15 Hi Llc Photodetector systems with low-power time-to-digital converter architectures to determine an arrival time of photon at a photodetector based on event detection time window
US11883181B2 (en) 2020-02-21 2024-01-30 Hi Llc Multimodal wearable measurement systems and methods
US11515014B2 (en) 2020-02-21 2022-11-29 Hi Llc Methods and systems for initiating and conducting a customized computer-enabled brain research study
US12029558B2 (en) 2020-02-21 2024-07-09 Hi Llc Time domain-based optical measurement systems and methods configured to measure absolute properties of tissue
US11969259B2 (en) 2020-02-21 2024-04-30 Hi Llc Detector assemblies for a wearable module of an optical measurement system and including spring-loaded light-receiving members
US11630310B2 (en) 2020-02-21 2023-04-18 Hi Llc Wearable devices and wearable assemblies with adjustable positioning for use in an optical measurement system
US11950879B2 (en) 2020-02-21 2024-04-09 Hi Llc Estimation of source-detector separation in an optical measurement system
US11771362B2 (en) 2020-02-21 2023-10-03 Hi Llc Integrated detector assemblies for a wearable module of an optical measurement system
US11096620B1 (en) 2020-02-21 2021-08-24 Hi Llc Wearable module assemblies for an optical measurement system
US11645483B2 (en) 2020-03-20 2023-05-09 Hi Llc Phase lock loop circuit based adjustment of a measurement time window in an optical measurement system
US11864867B2 (en) 2020-03-20 2024-01-09 Hi Llc Control circuit for a light source in an optical measurement system by applying voltage with a first polarity to start an emission of a light pulse and applying voltage with a second polarity to stop the emission of the light pulse
US11877825B2 (en) 2020-03-20 2024-01-23 Hi Llc Device enumeration in an optical measurement system
US11857348B2 (en) 2020-03-20 2024-01-02 Hi Llc Techniques for determining a timing uncertainty of a component of an optical measurement system
US11903676B2 (en) 2020-03-20 2024-02-20 Hi Llc Photodetector calibration of an optical measurement system
US11819311B2 (en) 2020-03-20 2023-11-21 Hi Llc Maintaining consistent photodetector sensitivity in an optical measurement system
US11245404B2 (en) 2020-03-20 2022-02-08 Hi Llc Phase lock loop circuit based signal generation in an optical measurement system
US11607132B2 (en) 2020-03-20 2023-03-21 Hi Llc Temporal resolution control for temporal point spread function generation in an optical measurement system
US11187575B2 (en) 2020-03-20 2021-11-30 Hi Llc High density optical measurement systems with minimal number of light sources
US12059262B2 (en) 2020-03-20 2024-08-13 Hi Llc Maintaining consistent photodetector sensitivity in an optical measurement system
US12085789B2 (en) 2020-03-20 2024-09-10 Hi Llc Bias voltage generation in an optical measurement system
US12059270B2 (en) 2020-04-24 2024-08-13 Hi Llc Systems and methods for noise removal in an optical measurement system

Similar Documents

Publication Publication Date Title
WO2008144831A1 (fr) Procédé et appareil d'inspection de tissu
US8553337B2 (en) Multi-path, multi-magnification, non-confocal fluorescence emission endoscopy apparatus and methods
US7702381B2 (en) Optical fiber delivery and collection method for biological applications such as multiphoton microscopy, spectroscopy, and endoscopy
US8705184B2 (en) Multi-path, multi-magnification, non-confocal fluorescence emission endoscopy apparatus and methods
US9615748B2 (en) Endoscopic biopsy apparatus, system and method
CN106037831B (zh) 实现增强信噪比的多光子光学成像的光纤光学装置
US20090326385A1 (en) Obtaining optical tissue properties
US20130324858A1 (en) Multi-path, multi-magnification, non-confocal fluorescence emission endoscopy apparatus and methods
US20230333360A1 (en) Point-Of-Care Microscope for Real-Time Acquisition of Volumetric Histological Images In Vivo
JP6389828B2 (ja) 医用撮像装置と共に使用するための光学装置
Vega et al. Triple-modality co-registered endoscope featuring wide-field reflectance imaging, and high-resolution multiphoton and optical coherence microscopy
US8923955B2 (en) Use of a system for imaging by fiber-optic confocal fluorescence in vivo in situ, system and method for imaging by fiber-optic confocal fluorescence in vivo in situ
CN110916728A (zh) 基于光纤传输型荧光寿命引导的穿刺活检方法及装置
JP2017508573A (ja) 材料に挿入して材料検体を採取するための医療装置およびその方法
US7885011B1 (en) Objective for optical imaging systems
Weinigel et al. Clinical multiphoton endoscopy with FLIM capability
Camli et al. Two photon imaging probe with highly efficient autofluorescence collection at high scattering and deep imaging conditions
Rivera et al. Multiphoton endoscopes for in vivo imaging of unstained tissues
Chen Fiber-optic Raman Sensing on Tissue Identification and Bladder Cancer Diagnosis
RU51847U1 (ru) Насадка на световод
Zvyagin Multiphoton endoscopy
Civiac Confocal Microendoscopy
McMullen Improving the contrast of two-photon microscopy and the application of fluorescence methods to the detection of tuberculosis
Pierce et al. Clinical evaluation of a high-resolution microendoscope for early diagnosis of cancer
Huland et al. CLINICAL ENDOSCOPY/MULTIPHOTON IMAGING/FIBER OPTICS: Multiphoton endoscopy for clinical applications

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 08748024

Country of ref document: EP

Kind code of ref document: A1

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 08748024

Country of ref document: EP

Kind code of ref document: A1