WO2008139421A3 - Electrical transduction method and device for the detection of biorecognition events in biomolecular interaction processes for genome/proteome analysis - Google Patents
Electrical transduction method and device for the detection of biorecognition events in biomolecular interaction processes for genome/proteome analysis Download PDFInfo
- Publication number
- WO2008139421A3 WO2008139421A3 PCT/IB2008/051900 IB2008051900W WO2008139421A3 WO 2008139421 A3 WO2008139421 A3 WO 2008139421A3 IB 2008051900 W IB2008051900 W IB 2008051900W WO 2008139421 A3 WO2008139421 A3 WO 2008139421A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- biorecognition
- electrodes
- conductive
- nanojunction
- interact
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 4
- 230000003993 interaction Effects 0.000 title abstract 2
- 108010026552 Proteome Proteins 0.000 title 1
- 238000001514 detection method Methods 0.000 title 1
- 230000026683 transduction Effects 0.000 title 1
- 238000010361 transduction Methods 0.000 title 1
- 239000011370 conductive nanoparticle Substances 0.000 abstract 3
- 239000002105 nanoparticle Substances 0.000 abstract 3
- 230000008878 coupling Effects 0.000 abstract 2
- 238000010168 coupling process Methods 0.000 abstract 2
- 238000005859 coupling reaction Methods 0.000 abstract 2
- 239000003068 molecular probe Substances 0.000 abstract 2
- 239000000523 sample Substances 0.000 abstract 2
Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y5/00—Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y10/00—Nanotechnology for information processing, storage or transmission, e.g. quantum computing or single electron logic
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6825—Nucleic acid detection involving sensors
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
- G01N27/30—Electrodes, e.g. test electrodes; Half-cells
- G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
- G01N27/3275—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction
- G01N27/3278—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction involving nanosized elements, e.g. nanogaps or nanoparticles
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54366—Apparatus specially adapted for solid-phase testing
- G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
- G01N33/5438—Electrodes
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/585—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with a particulate label, e.g. coloured latex
- G01N33/587—Nanoparticles
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- H—ELECTRICITY
- H10—SEMICONDUCTOR DEVICES; ELECTRIC SOLID-STATE DEVICES NOT OTHERWISE PROVIDED FOR
- H10K—ORGANIC ELECTRIC SOLID-STATE DEVICES
- H10K10/00—Organic devices specially adapted for rectifying, amplifying, oscillating or switching; Organic capacitors or resistors having potential barriers
- H10K10/701—Organic molecular electronic devices
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- H—ELECTRICITY
- H10—SEMICONDUCTOR DEVICES; ELECTRIC SOLID-STATE DEVICES NOT OTHERWISE PROVIDED FOR
- H10K—ORGANIC ELECTRIC SOLID-STATE DEVICES
- H10K85/00—Organic materials used in the body or electrodes of devices covered by this subclass
- H10K85/761—Biomolecules or bio-macromolecules, e.g. proteins, chlorophyl, lipids or enzymes
Landscapes
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Nanotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Urology & Nephrology (AREA)
- Biotechnology (AREA)
- Hematology (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Organic Chemistry (AREA)
- Pathology (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Wood Science & Technology (AREA)
- Food Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Cell Biology (AREA)
- General Engineering & Computer Science (AREA)
- Crystallography & Structural Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Materials Engineering (AREA)
- Electrochemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Pharmacology & Pharmacy (AREA)
- Medical Informatics (AREA)
- Genetics & Genomics (AREA)
- Mathematical Physics (AREA)
- Theoretical Computer Science (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analyzing Materials By The Use Of Magnetic Means (AREA)
Abstract
A method is described for detecting biorecognition events in biomolecular interaction processes, including the provision, on at least one biorecognition site, of molecular probes (P) adapted to interact with target molecular species (T) which are coupled to conductive nanoparticles (NP), in which the probes (P) are associated with a nanojunction transducer device (12) comprising a pair of conductive electrodes (22, 24) separated by a nanometric gap of the same order of magnitude as the dimensions of said conductive nanoparticles (NP), adapted to detect a condition of coupling between at least one molecular probe (P) and a specimen of a target species (T) which interact with each other. A biorecognition event includes the coupling of a probe (P) to at least one specimen of the target species (T) and results in the positioning of the conductive nanoparticle (NP) coupled to the target species (T) (by being directly conjugated with the latter or with a signal molecule which can interact with it) between the electrodes (22, 24) of the nanojunction device (12), with the consequent creation of a conductive path between the electrodes. The occurrence of biorecognition events is evaluated according to the intensity of the current which is made to flow between the electrodes (22, 24) of the nanojunction device (12) by the application of a potential difference.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
IT000341A ITTO20070341A1 (en) | 2007-05-15 | 2007-05-15 | PROCEDURE AND DEVICE FOR ELECTRIC TRANSDUCTION FOR THE DETECTION OF BIO-RECOGNITION EVENTS IN BIOMOLECULAR INTERACTION PROCESSES FOR GENOMIC / PROTEOMIC ANALYSIS |
ITTO2007A000341 | 2007-05-15 |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2008139421A2 WO2008139421A2 (en) | 2008-11-20 |
WO2008139421A3 true WO2008139421A3 (en) | 2009-03-26 |
Family
ID=39968023
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/IB2008/051900 WO2008139421A2 (en) | 2007-05-15 | 2008-05-14 | Electrical transduction method and device for the detection of biorecognition events in biomolecular interaction processes for genome/proteome analysis |
Country Status (2)
Country | Link |
---|---|
IT (1) | ITTO20070341A1 (en) |
WO (1) | WO2008139421A2 (en) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP7174065B2 (en) * | 2018-04-17 | 2022-11-17 | コリア リサーチ インスティテュート オブ ケミカル テクノロジー | Multiwell electrode-based biosensor |
Citations (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5191784A (en) * | 1989-12-28 | 1993-03-09 | Siemens Corporate Research, Inc. | Opto-electronic gas sensor |
WO2001044501A2 (en) * | 1999-12-13 | 2001-06-21 | november Aktiengesellschaft Gesellschaft für Molekulare Medizin | Method and device for detecting and quantifying biomolecules |
WO2001073123A2 (en) * | 2000-03-28 | 2001-10-04 | Nanosphere Inc. | Nanoparticles having oligonucleotides attached thereto and uses therefor |
EP1231214A2 (en) * | 2001-02-01 | 2002-08-14 | Hitachi Software Engineering Co., Ltd. | Biopolymer detector |
WO2004036217A1 (en) * | 2002-10-12 | 2004-04-29 | Fujitsu Limited | Semiconductor base structure for molecular electronics and molecular electronic-based biosensor devices and a method for producing such a semiconductor base structure |
WO2005008450A2 (en) * | 2003-03-28 | 2005-01-27 | The Regents Of The University Of California | Method and apparatus for nanogap device and array |
WO2005108612A2 (en) * | 2003-11-28 | 2005-11-17 | Genorx, Inc. | Nanoscale biosensor device, system and technique |
WO2006067276A1 (en) * | 2004-12-23 | 2006-06-29 | Nanolab Systems Oy | Utilization of nanowires in chemical and biological analysis |
-
2007
- 2007-05-15 IT IT000341A patent/ITTO20070341A1/en unknown
-
2008
- 2008-05-14 WO PCT/IB2008/051900 patent/WO2008139421A2/en active Application Filing
Patent Citations (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5191784A (en) * | 1989-12-28 | 1993-03-09 | Siemens Corporate Research, Inc. | Opto-electronic gas sensor |
WO2001044501A2 (en) * | 1999-12-13 | 2001-06-21 | november Aktiengesellschaft Gesellschaft für Molekulare Medizin | Method and device for detecting and quantifying biomolecules |
WO2001073123A2 (en) * | 2000-03-28 | 2001-10-04 | Nanosphere Inc. | Nanoparticles having oligonucleotides attached thereto and uses therefor |
EP1231214A2 (en) * | 2001-02-01 | 2002-08-14 | Hitachi Software Engineering Co., Ltd. | Biopolymer detector |
WO2004036217A1 (en) * | 2002-10-12 | 2004-04-29 | Fujitsu Limited | Semiconductor base structure for molecular electronics and molecular electronic-based biosensor devices and a method for producing such a semiconductor base structure |
WO2005008450A2 (en) * | 2003-03-28 | 2005-01-27 | The Regents Of The University Of California | Method and apparatus for nanogap device and array |
WO2005108612A2 (en) * | 2003-11-28 | 2005-11-17 | Genorx, Inc. | Nanoscale biosensor device, system and technique |
WO2006067276A1 (en) * | 2004-12-23 | 2006-06-29 | Nanolab Systems Oy | Utilization of nanowires in chemical and biological analysis |
Non-Patent Citations (2)
Title |
---|
SEBASTIAN M LUBER ET AL: "Nanometre spaced electrodes on a cleaved AlGaAs surface; Nanometre spaced electrodes on a cleaved AlGaAs surface", NANOTECHNOLOGY, IOP, BRISTOL, GB, vol. 16, no. 8, 1 August 2005 (2005-08-01), pages 1182 - 1185, XP020091195, ISSN: 0957-4484 * |
TREMBLAY F ET AL: "DESIGN NOTE; Hopping in a low-mobility GaAs-AlGaAs heterojunction in the limit of low electronic concentrations", JOURNAL OF PHYSICS: CONDENSED MATTER, INSTITUTE OF PHYSICS PUBLISHING, BRISTOL, GB, vol. 2, no. 35, 3 September 1990 (1990-09-03), pages 7367 - 7371, XP020058664, ISSN: 0953-8984 * |
Also Published As
Publication number | Publication date |
---|---|
WO2008139421A2 (en) | 2008-11-20 |
ITTO20070341A1 (en) | 2008-11-16 |
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