WO2008134830A1 - Procédé pour obtenir le lipide monophosphorylé a à partir de bordetella pertussis comme sous-produit de la production du vaccin cellulaire anti-coquelucheux - Google Patents

Procédé pour obtenir le lipide monophosphorylé a à partir de bordetella pertussis comme sous-produit de la production du vaccin cellulaire anti-coquelucheux Download PDF

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Publication number
WO2008134830A1
WO2008134830A1 PCT/BR2007/000073 BR2007000073W WO2008134830A1 WO 2008134830 A1 WO2008134830 A1 WO 2008134830A1 BR 2007000073 W BR2007000073 W BR 2007000073W WO 2008134830 A1 WO2008134830 A1 WO 2008134830A1
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WO
WIPO (PCT)
Prior art keywords
vaccine
bordetella pertussis
bpmpla
final bulk
product
Prior art date
Application number
PCT/BR2007/000073
Other languages
English (en)
Inventor
Isaias Raw
Flávia Saldanha KUBRUSLY
Dmitri Iourtov
Maria Aparecida Sakauchi
Fernanda Lúcio dos SANTOS
Elaine Darini
Noemi Furuyama
Sally Müller Affonso PRADO
Hisako Gondo Higashi
Sandra de Cássia DIAS
Original Assignee
Fundação Butantan
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Fundação Butantan filed Critical Fundação Butantan
Priority to BRPI0721393A priority Critical patent/BRPI0721393B8/pt
Priority to PCT/BR2007/000073 priority patent/WO2008134830A1/fr
Publication of WO2008134830A1 publication Critical patent/WO2008134830A1/fr

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/12—Viral antigens
    • A61K39/145—Orthomyxoviridae, e.g. influenza virus
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/12—Viral antigens
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/39—Medicinal preparations containing antigens or antibodies characterised by the immunostimulating additives, e.g. chemical adjuvants
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H13/00—Compounds containing saccharide radicals esterified by carbonic acid or derivatives thereof, or by organic acids, e.g. phosphonic acids
    • C07H13/02—Compounds containing saccharide radicals esterified by carbonic acid or derivatives thereof, or by organic acids, e.g. phosphonic acids by carboxylic acids
    • C07H13/04—Compounds containing saccharide radicals esterified by carbonic acid or derivatives thereof, or by organic acids, e.g. phosphonic acids by carboxylic acids having the esterifying carboxyl radicals attached to acyclic carbon atoms
    • C07H13/06—Fatty acids
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/555—Medicinal preparations containing antigens or antibodies characterised by a specific combination antigen/adjuvant
    • A61K2039/55511—Organic adjuvants
    • A61K2039/55572—Lipopolysaccharides; Lipid A; Monophosphoryl lipid A
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011—Details
    • C12N2760/16011—Orthomyxoviridae
    • C12N2760/16034—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Public Health (AREA)
  • Virology (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Pulmonology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

L'invention porte sur un procédé pour obtenir le Lipide monophosphorylé A à partir de Bordetella pertussis (BpMPLA), comme sous-produit de la production du vaccin cellulaire anticoquelucheux, en vue d'une utilisation dans des applications immunostimulantes telles qu'un adjuvant de vaccin, et, plus particulièrement, sur un procédé de purification de BpMPLA à partir du traitement d'un extrait alcoolique, d'un surnageant ou d'un filtrat habituellement rejeté provenant de la masse finale du vaccin contre Bordetella pertussis (sous-produit de la fabrication du vaccin principal). Ce procédé de purification de BpMPLA comprend le traitement d'un extrait alcoolique habituellement rejeté et provenant de la masse finale du vaccin contre Bordetella pertussis, les opérations consistant à soumettre cette masse finale à une colonne d'affinité et à soumettre la fraction éluée par détergent à une hydrolyse acide, suivie d'une neutralisation, d'une lyophilisation et d'une remise en suspension avec de l'eau pour injection plus un détergent doux. Le nouveau BpMPLA est utilisé dans des applications immunostimulantes, plus particulièrement, en tant qu'adjuvant de vaccin, et, de façon encore plus particulière, en tant qu'adjuvant pour le vaccin contre la grippe, avec des résultats prometteurs.
PCT/BR2007/000073 2007-03-22 2007-03-22 Procédé pour obtenir le lipide monophosphorylé a à partir de bordetella pertussis comme sous-produit de la production du vaccin cellulaire anti-coquelucheux WO2008134830A1 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
BRPI0721393A BRPI0721393B8 (pt) 2007-03-22 2007-03-22 método para obter lipídeo a monofosforilado da bordetella pertussis como um subproduto da produção de vacina celular contra coqueluche
PCT/BR2007/000073 WO2008134830A1 (fr) 2007-03-22 2007-03-22 Procédé pour obtenir le lipide monophosphorylé a à partir de bordetella pertussis comme sous-produit de la production du vaccin cellulaire anti-coquelucheux

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/BR2007/000073 WO2008134830A1 (fr) 2007-03-22 2007-03-22 Procédé pour obtenir le lipide monophosphorylé a à partir de bordetella pertussis comme sous-produit de la production du vaccin cellulaire anti-coquelucheux

Publications (1)

Publication Number Publication Date
WO2008134830A1 true WO2008134830A1 (fr) 2008-11-13

Family

ID=39943063

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/BR2007/000073 WO2008134830A1 (fr) 2007-03-22 2007-03-22 Procédé pour obtenir le lipide monophosphorylé a à partir de bordetella pertussis comme sous-produit de la production du vaccin cellulaire anti-coquelucheux

Country Status (2)

Country Link
BR (1) BRPI0721393B8 (fr)
WO (1) WO2008134830A1 (fr)

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2220211A (en) * 1988-06-29 1990-01-04 Ribi Immunochem Research Inc Modified lipopolysaccharides
WO1994019013A1 (fr) * 1993-02-19 1994-09-01 Smithkline Beecham Corporation Compositions de vaccin contre la grippe, contenant un lipide a de monophosphoryle desacyle en position 3-o
US6013640A (en) * 1998-08-21 2000-01-11 Ribi Immunochem Research, Inc. Phosphoglycolipid and methods for its use
WO2002078637A2 (fr) * 2001-03-30 2002-10-10 Corixa Corporation Procedes de production de 3-o-desactive-4'-lipide a monophosphoryl (3d-mla)
BR0303762A (pt) * 2003-08-29 2005-04-12 Xirlene Santos Salgueiro Processos de obtenção do lipopolissacarìdeo de bordetella pertussis e de obtenção e preparação do lipìdeo a monofosforilado de bordetella pertussis, e de uso do lipìdeo a monofosforilado de bordetella pertussus como adjuvante em imunizações e vacinas
BR0303416A (pt) * 2003-08-21 2005-05-10 Univ Fed Sao Paulo Unifesp Processos de obtenção do lipopolissacarìdeo de bordetella pertussis e de obtenção e preparação do lipìdeo-a monofosforilado de bordetella pertussis, uso do lipìdeo-a monofosforilado de bordetella pertussis e vacinas

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2220211A (en) * 1988-06-29 1990-01-04 Ribi Immunochem Research Inc Modified lipopolysaccharides
WO1994019013A1 (fr) * 1993-02-19 1994-09-01 Smithkline Beecham Corporation Compositions de vaccin contre la grippe, contenant un lipide a de monophosphoryle desacyle en position 3-o
US6013640A (en) * 1998-08-21 2000-01-11 Ribi Immunochem Research, Inc. Phosphoglycolipid and methods for its use
WO2002078637A2 (fr) * 2001-03-30 2002-10-10 Corixa Corporation Procedes de production de 3-o-desactive-4'-lipide a monophosphoryl (3d-mla)
BR0303416A (pt) * 2003-08-21 2005-05-10 Univ Fed Sao Paulo Unifesp Processos de obtenção do lipopolissacarìdeo de bordetella pertussis e de obtenção e preparação do lipìdeo-a monofosforilado de bordetella pertussis, uso do lipìdeo-a monofosforilado de bordetella pertussis e vacinas
BR0303762A (pt) * 2003-08-29 2005-04-12 Xirlene Santos Salgueiro Processos de obtenção do lipopolissacarìdeo de bordetella pertussis e de obtenção e preparação do lipìdeo a monofosforilado de bordetella pertussis, e de uso do lipìdeo a monofosforilado de bordetella pertussus como adjuvante em imunizações e vacinas

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
DATABASE CAPLUS [online] accession no. STN Database accession no. (2005:1197878) *
DATABASE CAPLUS [online] accession no. STN Database accession no. (2006:1266855) *
DATABASE CAPLUS [online] QURESHI N. ET AL.: "Monophosphoryl lipid A obtained from lipopolysaccharides of Salmonella minnesota R595. Purification of the dimethyl derivative by high performance liquid chromatography and complete structural determination", accession no. STN Database accession no. (1985:419515) *
DATABASE CAPLUS [online] QURESHI N. ET AL.: "Purification and structural determination of nontoxic lipid A obtained from the lipopolysaccharide of Salmonella typhimurium", accession no. STN Database accession no. (1982:576580) *
JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 257, no. 19, 1982, pages 11808 - 11815 *
JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 260, no. 9, 1985, pages 5271 - 5278 *

Also Published As

Publication number Publication date
BRPI0721393B8 (pt) 2022-09-06
BRPI0721393B1 (pt) 2020-08-18
BRPI0721393A2 (pt) 2013-01-01

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