WO2008133206A1 - Non-replicating paramyxoviridae virus vector - Google Patents

Non-replicating paramyxoviridae virus vector Download PDF

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Publication number
WO2008133206A1
WO2008133206A1 PCT/JP2008/057609 JP2008057609W WO2008133206A1 WO 2008133206 A1 WO2008133206 A1 WO 2008133206A1 JP 2008057609 W JP2008057609 W JP 2008057609W WO 2008133206 A1 WO2008133206 A1 WO 2008133206A1
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WO
WIPO (PCT)
Prior art keywords
gene
vector
replicating
sev
cell
Prior art date
Application number
PCT/JP2008/057609
Other languages
French (fr)
Japanese (ja)
Inventor
Makoto Inoue
Hiroshi Ban
Mamoru Hasegawa
Yutaka Hanazono
Yukiko Kishi
Original Assignee
Dnavec Corporation
Educational Foundation Jichi Medical University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dnavec Corporation, Educational Foundation Jichi Medical University filed Critical Dnavec Corporation
Publication of WO2008133206A1 publication Critical patent/WO2008133206A1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/60—Fusion polypeptide containing spectroscopic/fluorescent detection, e.g. green fluorescent protein [GFP]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/61—Fusion polypeptide containing an enzyme fusion for detection (lacZ, luciferase)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/10011—Adenoviridae
    • C12N2710/10041—Use of virus, viral particle or viral elements as a vector
    • C12N2710/10043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011—Details
    • C12N2760/18011—Paramyxoviridae
    • C12N2760/18811—Sendai virus
    • C12N2760/18851—Methods of production or purification of viral material
    • C12N2760/18852—Methods of production or purification of viral material relating to complementing cells and packaging systems for producing virus or viral particles
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/30—Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Virology (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

A non-replicating SeV vector (SeV/ΔP vector) can be successfully produced, which has the deletion of a gene for P-protein that is a protein constituting an RNP from genomic RNA or the inactivation of the gene. It is found that an SeV vector which has the deletion or inactivation of P gene and carries a marker gene such as a gene for GFP or luciferase can be used for achieving a high transfer efficiency of a foreign gene into a cell or a high expression efficiency of a foreign gene in a cell. It is also found that the vector has reduced cytotoxicity, because P gene is deleted or inactivated in the vector to cause the reduction of the amount of a virus-derived protein expressed in a host cell.
PCT/JP2008/057609 2007-04-19 2008-04-18 Non-replicating paramyxoviridae virus vector WO2008133206A1 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
JP2007-110547 2007-04-19
JP2007110547 2007-04-19
JP2007127421 2007-05-11
JP2007-127421 2007-05-11

Publications (1)

Publication Number Publication Date
WO2008133206A1 true WO2008133206A1 (en) 2008-11-06

Family

ID=39925660

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2008/057609 WO2008133206A1 (en) 2007-04-19 2008-04-18 Non-replicating paramyxoviridae virus vector

Country Status (1)

Country Link
WO (1) WO2008133206A1 (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010008054A1 (en) * 2008-07-16 2010-01-21 ディナベック株式会社 Method for production of reprogrammed cell using chromosomally unintegrated virus vector
WO2016125364A1 (en) * 2015-02-02 2016-08-11 株式会社Ｉｄファーマ Improved negative-strand rna viral vector

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2005065596A (en) * 2003-08-25 2005-03-17 Japan Science & Technology Agency Proliferation potency-deficient rabies virus
WO2007007921A1 (en) * 2005-07-13 2007-01-18 Kyushu University, National University Corporation Segmented genome type recombinant mononegavirus vector

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2005065596A (en) * 2003-08-25 2005-03-17 Japan Science & Technology Agency Proliferation potency-deficient rabies virus
WO2007007921A1 (en) * 2005-07-13 2007-01-18 Kyushu University, National University Corporation Segmented genome type recombinant mononegavirus vector

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
HANAZONO Y.: "Shinki Sendai Virus Vector o Mochiita Saitaiketsu Kansaibo Zofukuho no Kaihatsu", SHINKI SENDAI VIRUS VECTOR O MOCHIITA SAITAIKETSU KANSAIBO ZOFUKUHO NO KAIHATSU HEISEI 18 NENDO SOKATSU. BUNTAN KENKYU HOKOKUSHO, March 2007 (2007-03-01), pages 1 - 7 *
INOUE M.: "HoxB4 Idenshi o Tosao suru P Kessongata SeV Vector no Sakusei to Seino Hyoka ni Kansuru Kenkyu", SHINKI SENDAI VIRUS VECTOR O MOCHIITA SAITAIKETSU KANSAIBO ZOFUKUHO NO KAIHATSU HEISEI 18 NENDO SOKATSU. BUNTAN KENKYU HOKOKUSHO, March 2007 (2007-03-01), pages 8 - 11 *
MORIMOTO K.: "Virus Vector o Oyo shita Vaccine Kaihatsu Jinsokuka no Tameno Kibanteki Gijutsu Kaihatsu no Kenkyu", VIRUS VECTOR O OYO SHITA VACCINE KAIHATSU JINSOKUKA NO TAMENO KIBANTEKI GIJUTSU KAIHATSU NO KENKYU HEISEI 17 NENDO SOKATSU. BUNTAN KENKYU HOKOKUSHO, 2006, pages 1 - 15 *
WIEGAND M.A. ET AL.: "De novo synthesis of N and P proteins as a key step in Sendai virus gene expression", J. VIROL., vol. 81, no. 24, pages 13835 - 13844, XP002684869, DOI: doi:10.1128/JVI.00914-07 *

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010008054A1 (en) * 2008-07-16 2010-01-21 ディナベック株式会社 Method for production of reprogrammed cell using chromosomally unintegrated virus vector
US9127256B2 (en) 2008-07-16 2015-09-08 Dnavec Corporation Method for production of reprogrammed cell using chromosomally unintegrated virus vector
US9695445B2 (en) 2008-07-16 2017-07-04 Id Pharma Co., Ltd. Method for production of reprogrammed cell using chromosomally unintegrated virus vector
US11136594B2 (en) 2008-07-16 2021-10-05 Id Pharma Co., Ltd. Method for production of reprogrammed cell using chromosomally unintegrated virus vector
WO2016125364A1 (en) * 2015-02-02 2016-08-11 株式会社Ｉｄファーマ Improved negative-strand rna viral vector
JPWO2016125364A1 (en) * 2015-02-02 2017-11-30 株式会社Ｉｄファーマ Improved minus-strand RNA viral vector
JP2020195381A (en) * 2015-02-02 2020-12-10 株式会社Ｉｄファーマ Improved negative-strand RNA virus vector

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