WO2008130027A1 - Improved bacterial two-hybrid assay - Google Patents

Improved bacterial two-hybrid assay Download PDF

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Publication number
WO2008130027A1
WO2008130027A1 PCT/JP2008/057587 JP2008057587W WO2008130027A1 WO 2008130027 A1 WO2008130027 A1 WO 2008130027A1 JP 2008057587 W JP2008057587 W JP 2008057587W WO 2008130027 A1 WO2008130027 A1 WO 2008130027A1
Authority
WO
WIPO (PCT)
Prior art keywords
hybrid assay
bacterial
substance
improved bacterial
interacting
Prior art date
Application number
PCT/JP2008/057587
Other languages
French (fr)
Japanese (ja)
Inventor
Takachika Azuma
Akikazu Murakami
Original Assignee
Kyokuto Pharmaceutical Industrial Co., Ltd.
Tokyo University Of Science
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kyokuto Pharmaceutical Industrial Co., Ltd., Tokyo University Of Science filed Critical Kyokuto Pharmaceutical Industrial Co., Ltd.
Priority to JP2009510863A priority Critical patent/JPWO2008130027A1/en
Publication of WO2008130027A1 publication Critical patent/WO2008130027A1/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1055—Protein x Protein interaction, e.g. two hybrid selection
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value
    • G01N2500/04—Screening involving studying the effect of compounds C directly on molecule A (e.g. C are potential ligands for a receptor A, or potential substrates for an enzyme A)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Virology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Disclosed is a method for rapidly and efficiently screening a substance (e.g., an antibody, a peptide) capable of interacting with a target substance. Specifically disclosed are: a novel bacterial two-hybrid assay utilizing the gene transfer by a phage; and a screening method comprising a combination of the bacterial two-hybrid assay with a phage display system. The bacterial two-hybrid assay and the screening method enable to rapidly and efficiently screen a substance (e.g., an antibody, a peptide) capable of interacting with a target substance.
PCT/JP2008/057587 2007-04-18 2008-04-18 Improved bacterial two-hybrid assay WO2008130027A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2009510863A JPWO2008130027A1 (en) 2007-04-18 2008-04-18 Improved bacterial two-hybrid method

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007109391 2007-04-18
JP2007-109391 2007-04-18

Publications (1)

Publication Number Publication Date
WO2008130027A1 true WO2008130027A1 (en) 2008-10-30

Family

ID=39875549

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2008/057587 WO2008130027A1 (en) 2007-04-18 2008-04-18 Improved bacterial two-hybrid assay

Country Status (2)

Country Link
JP (1) JPWO2008130027A1 (en)
WO (1) WO2008130027A1 (en)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2002000272A (en) * 2000-06-21 2002-01-08 Mitsubishi-Tokyo Pharmaceuticals Inc Expression vector for phage display method, and its utilization
WO2004016782A1 (en) * 2002-08-16 2004-02-26 The University Of Tokyo Method of assaying interaction between proteins
JP2005323522A (en) * 2004-05-13 2005-11-24 Kazusa Dna Kenkyusho Escherichia coli two-hybrid system by means of liquid culture

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2002000272A (en) * 2000-06-21 2002-01-08 Mitsubishi-Tokyo Pharmaceuticals Inc Expression vector for phage display method, and its utilization
WO2004016782A1 (en) * 2002-08-16 2004-02-26 The University Of Tokyo Method of assaying interaction between proteins
JP2005323522A (en) * 2004-05-13 2005-11-24 Kazusa Dna Kenkyusho Escherichia coli two-hybrid system by means of liquid culture

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
ALLEN J.B. ET AL.: "Finding prospective partners in the library: the two-hybrid system and phage display find a match", TRENDS BIOCHEM. SCI., vol. 20, no. 12, 1995, pages 511 - 516, XP000198723 *
BAIR C.L. ET AL.: "A phage display system designed to detect and study protein-protein interactions", MOL. MICROBIOL., vol. 67, no. 4, February 2008 (2008-02-01), pages 719 - 728, XP003021951 *
FAZI B. ET AL.: "Unusual Binding Properties of the SH3 Domain of the Yeast Actin-binding Protein Abp1", J. BIOL. CHEM., vol. 277, no. 7, 2002, pages 5290 - 5298, XP003021949 *
GERMINO F.J. ET AL.: "Screening for in vivo protein-protein interactions", PROC. NATL. ACAD. SCI. U.S.A., vol. 90, no. 3, 1993, pages 933 - 937, XP002095757 *
MOSSNER E. ET AL.: "Fast Selection of Antibodies without Antigen Purification: Adaptation of the Protein Fragment Complementation Assay to Select Antigen-Antibody Pairs", J. MOL. BIOL., vol. 308, 2001, pages 115 - 122, XP004479173 *
PORTNER-TALIANA A. ET AL.: "In vivo selection of single-chain antibodies using a yeast two-hybrid system", J. IMMUNOL. METHODS, vol. 238, 2000, pages 161 - 172, XP004195472 *
ZHANG L. ET AL.: "Molecular Profiling of Heart Endothelial Cells", CIRCULATION, vol. 112, 2005, pages 1601 - 1611, XP003021950 *

Also Published As

Publication number Publication date
JPWO2008130027A1 (en) 2010-07-22

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