WO2008127193A8 - Metalled sirnas for facilitated targeting of suboptimal mrna sequences - Google Patents

Metalled sirnas for facilitated targeting of suboptimal mrna sequences Download PDF

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Publication number
WO2008127193A8
WO2008127193A8 PCT/SE2008/050436 SE2008050436W WO2008127193A8 WO 2008127193 A8 WO2008127193 A8 WO 2008127193A8 SE 2008050436 W SE2008050436 W SE 2008050436W WO 2008127193 A8 WO2008127193 A8 WO 2008127193A8
Authority
WO
WIPO (PCT)
Prior art keywords
sirnas
targeting
metalled
suboptimal
facilitated
Prior art date
Application number
PCT/SE2008/050436
Other languages
French (fr)
Other versions
WO2008127193A1 (en
Inventor
Sofi Elmroth
Original Assignee
Forskarpatent I Syd Ab
Sofi Elmroth
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Forskarpatent I Syd Ab, Sofi Elmroth filed Critical Forskarpatent I Syd Ab
Publication of WO2008127193A1 publication Critical patent/WO2008127193A1/en
Publication of WO2008127193A8 publication Critical patent/WO2008127193A8/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A61P35/04—Antineoplastic agents specific for metastasis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering N.A.
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/33—Chemical structure of the base
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2320/00—Applications; Uses
    • C12N2320/50—Methods for regulating/modulating their activity

Abstract

The present invention relates to siRNAs with sense (passenger) strands deviating from that of a target gene by having introduced metallated bases in the sense (passenger) strand, as well as a method for locally decreasing the melting temperature and concomitant structural change of siRNA, and a method for targeting mRNA regions.
PCT/SE2008/050436 2007-04-17 2008-04-17 Metalled sirnas for facilitated targeting of suboptimal mrna sequences WO2008127193A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
SE0700944 2007-04-17
SE0700944-2 2007-04-17

Publications (2)

Publication Number Publication Date
WO2008127193A1 WO2008127193A1 (en) 2008-10-23
WO2008127193A8 true WO2008127193A8 (en) 2009-07-23

Family

ID=39864180

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/SE2008/050436 WO2008127193A1 (en) 2007-04-17 2008-04-17 Metalled sirnas for facilitated targeting of suboptimal mrna sequences

Country Status (1)

Country Link
WO (1) WO2008127193A1 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014170786A1 (en) 2013-04-17 2014-10-23 Pfizer Inc. N-piperidin-3-ylbenzamide derivatives for treating cardiovascular diseases

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005062937A2 (en) * 2003-12-22 2005-07-14 University Of Massachusetts Methods and compositions for enhancing the efficacy and specificity of single and double blunt-ended sirna
WO2005116226A2 (en) * 2004-05-24 2005-12-08 Midatech Ltd Nanoparticles comprising rna ligands

Also Published As

Publication number Publication date
WO2008127193A1 (en) 2008-10-23

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