WO2008120763A1 - ラン科植物の形質転換方法 - Google Patents

ラン科植物の形質転換方法 Download PDF

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Publication number
WO2008120763A1
WO2008120763A1 PCT/JP2008/056227 JP2008056227W WO2008120763A1 WO 2008120763 A1 WO2008120763 A1 WO 2008120763A1 JP 2008056227 W JP2008056227 W JP 2008056227W WO 2008120763 A1 WO2008120763 A1 WO 2008120763A1
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WO
WIPO (PCT)
Prior art keywords
family orchidaceae
plant
shoot
orchidaceae
family
Prior art date
Application number
PCT/JP2008/056227
Other languages
English (en)
French (fr)
Inventor
Yasunori Machida
Endang Semiarti
Chiyoko Machida
Original Assignee
National University Corporation Nagoya University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by National University Corporation Nagoya University filed Critical National University Corporation Nagoya University
Priority to JP2009507543A priority Critical patent/JPWO2008120763A1/ja
Publication of WO2008120763A1 publication Critical patent/WO2008120763A1/ja

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8205—Agrobacterium mediated transformation

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

本発明は、ラン科植物の形質転換体を効率的に取得するラン科植物の形質転換体の作製方法を提供することを目的とする。この目的のため、本発明では、所望の遺伝子が導入されたラン科植物組織を準備し、ラン科植物組織においてクラス１ＫＮＯＸ遺伝子の発現を促進して多芽体前駆体又は多芽体を誘導し、前記多芽体前駆体又は前記多芽体に形成されるシュート前駆体又はシュートから前記ラン科植物の個体を再生するようにする。
PCT/JP2008/056227 2007-03-31 2008-03-28 ラン科植物の形質転換方法 WO2008120763A1 (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2009507543A JPWO2008120763A1 (ja) 2007-03-31 2008-03-28 ラン科植物の形質転換方法

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007095868 2007-03-31
JP2007-095868 2007-03-31

Publications (1)

Publication Number Publication Date
WO2008120763A1 true WO2008120763A1 (ja) 2008-10-09

Family

ID=39808343

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2008/056227 WO2008120763A1 (ja) 2007-03-31 2008-03-28 ラン科植物の形質転換方法

Country Status (2)

Country Link
JP (1) JPWO2008120763A1 (ja)
WO (1) WO2008120763A1 (ja)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108719063A (zh) * 2018-05-16 2018-11-02 芜湖市三山区绿色食品产业协会 用于蝴蝶兰组织培养的组织培养基及其制备方法和应用
CN111197023A (zh) * 2018-11-16 2020-05-26 深圳华大生命科学研究院 一种兰科植物原生质体的制备及转化方法和应用

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113151353B (zh) * 2021-06-04 2022-04-22 中国农业科学院蔬菜花卉研究所 一种百合LlKnat13基因克隆及其在控制百合珠芽形成中的应用方法

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
LUO K. ET AL.: "The maize knotted1 gene is an effective positive selectable marker gene for Agrobacterium-mediated tobacco transformation", PLANT CELL REP., vol. 25, no. 5, 2006, pages 403 - 409, XP019335573 *
MISHIBA K. ET AL.: "Agrobacterium-mediated transformation of Phalaenopsis by targeting protocorms at an early stage after germination", PLANT CELL REP., vol. 24, 2005, pages 297 - 303, XP019335489 *
SEMIARTI E. ET AL.: "Agrobacterium-mediated transformation of the wild orchid species Phalaenopsis amabilis", PLANT BIOTECHNOL., vol. 24, no. 3, June 2007 (2007-06-01), pages 265 - 272 *
SEMIARTI E. ET AL.: "Shoot development programs in Phalaenopsis amabilis orchild plant", DAI 48 KAI PROCEEDINGS OF THE ANNUAL MEETING OF THE JAPANESE SOCIETY OF PLANT PHYSIOLOGISTS, 15 March 2007 (2007-03-15), pages 91 + ABSTR. NO. 1AA12(012) *
TEMJENSANGBA ET AL.: "Regeneration and mass multiplication of Arachnis labrosa (Lindl. ex Paxt.) Rechb: A rare and threatened orchid", CURR. SCI., vol. 88, 2005, pages 1966 - 1969 *
YU H. ET AL.: "Agrobacterium-mediated transformation of a Dendrobium orchid with the class 1 knox gene DOH1", PLANT CELL REP., vol. 20, 2001, pages 301 - 305 *
YU H. ET AL.: "DOH1, a class 1 knox gene, is required for maintenance of the basic plant architecture and floral transition in orchid", PLANT CELL, vol. 12, 2000, pages 2143 - 2159 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108719063A (zh) * 2018-05-16 2018-11-02 芜湖市三山区绿色食品产业协会 用于蝴蝶兰组织培养的组织培养基及其制备方法和应用
CN111197023A (zh) * 2018-11-16 2020-05-26 深圳华大生命科学研究院 一种兰科植物原生质体的制备及转化方法和应用

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Publication number Publication date
JPWO2008120763A1 (ja) 2010-07-15

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