WO2008111161A1 - 間葉系幹細胞の均質性識別方法、および、その方法を用いて単離された均質な間葉系幹細胞 - Google Patents

間葉系幹細胞の均質性識別方法、および、その方法を用いて単離された均質な間葉系幹細胞 Download PDF

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Publication number
WO2008111161A1
WO2008111161A1 PCT/JP2007/054869 JP2007054869W WO2008111161A1 WO 2008111161 A1 WO2008111161 A1 WO 2008111161A1 JP 2007054869 W JP2007054869 W JP 2007054869W WO 2008111161 A1 WO2008111161 A1 WO 2008111161A1
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WO
WIPO (PCT)
Prior art keywords
mesenchymal stem
stem cells
differentiation
uniformity
determining
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/JP2007/054869
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English (en)
French (fr)
Inventor
Yukio Kato
Masami Kanawa
Akira Igarashi
Maiko Hara
Koichiro Tsuji
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Hiroshima University NUC
Two Cells Co Ltd
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Hiroshima University NUC
Two Cells Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Hiroshima University NUC, Two Cells Co Ltd filed Critical Hiroshima University NUC
Priority to PCT/JP2007/054869 priority Critical patent/WO2008111161A1/ja
Publication of WO2008111161A1 publication Critical patent/WO2008111161A1/ja
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6881—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for tissue or cell typing, e.g. human leukocyte antigen [HLA] probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

継代培養された間葉系幹細胞が脂肪分化、骨分化及び軟骨分化の３つの方向に分化する分化能を有し、分化誘導しない限りいずれの分化もしない細胞（多機能間葉系幹細胞）であるか否かを識別することができる間葉系幹細胞の均質性識別方法を提供すること。本発明は、間葉系幹細胞の均質性を決定する方法であって、（ａ）間葉系幹細胞の母集団から複数のクローンを作製する工程；（ｂ）そのクローン及びその母集団について、所定の遺伝子群に関するmRNA発現強度を決定する工程；（ｃ）工程（ｂ）において決定されたmRNA発現強度と遺伝子群との関係を図式化する工程；および（ｄ）工程（ｃ）において取得された図式から該母集団の均質性を判別する工程、を包含する方法を提供する。
PCT/JP2007/054869 2007-03-12 2007-03-12 間葉系幹細胞の均質性識別方法、および、その方法を用いて単離された均質な間葉系幹細胞 Ceased WO2008111161A1 (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PCT/JP2007/054869 WO2008111161A1 (ja) 2007-03-12 2007-03-12 間葉系幹細胞の均質性識別方法、および、その方法を用いて単離された均質な間葉系幹細胞

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/JP2007/054869 WO2008111161A1 (ja) 2007-03-12 2007-03-12 間葉系幹細胞の均質性識別方法、および、その方法を用いて単離された均質な間葉系幹細胞

Publications (1)

Publication Number Publication Date
WO2008111161A1 true WO2008111161A1 (ja) 2008-09-18

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Family Applications (1)

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PCT/JP2007/054869 Ceased WO2008111161A1 (ja) 2007-03-12 2007-03-12 間葉系幹細胞の均質性識別方法、および、その方法を用いて単離された均質な間葉系幹細胞

Country Status (1)

Country Link
WO (1) WO2008111161A1 (ja)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2014176364A (ja) * 2013-03-15 2014-09-25 Olympus Corp 幹細胞の解析方法
JP2016067311A (ja) * 2014-09-30 2016-05-09 株式会社ジーシー 軟骨分化培養液、及び軟骨組織
JP2018502556A (ja) * 2014-12-05 2018-02-01 メリディジェン・バイオテック・カンパニー・リミテッドＭｅｒｉｄｉｇｅｎ Ｂｉｏｔｅｃｈ Ｃｏ．， Ｌｔｄ． 間葉系幹細胞を区別する方法
CN110494556A (zh) * 2017-04-03 2019-11-22 株式会社钟化 包含间充质系干细胞的细胞群、其制造方法、以及医药组合物

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
"GeneChip Human Genome Array Series, Data Sheet", AFFYMETRIX, INC., 2003, XP003022966 *
ISHII M. ET AL.: "Molecular markers distinguish bone marrow mesenchymal stem cells from fibroblasts", BIOCHEM. BIOPHYS. RES. COMMUN., vol. 332, no. 1, 2005, pages 297 - 303, XP004892979 *
KATO Y. ET AL.: "Hito Kanyokei Kansaibo Baiyo to Hinshitsu Kanri no Strategy", BIOTECHNOLOGY JOURNAL, vol. 6, no. 6, 2006, pages 693 - 696, XP003022964 *
KATO Y. ET AL.: "II. Rinsho ni Muketa Oyo Nankotsu Shufuku ni Mochiirareru Kanyokei Kansaibo no Kihonteki Seishitsu (Characterization of transplantable mesenchymal stem cells that are useful for cartilage regeneration)", KANSETSU GEKA, vol. 25, no. 4, 2006, pages 63 - 69, XP003022963 *
SEGOE K. ET AL.: "Kanyokei Kansaibo no Tanpaku Marker Cytokine Teiryo ni yoru Approach", JOURNAL OF JAPANESE SOCIETY OF BONE MORPHOMETRY, vol. 16, no. 3, 2006, pages 67, XP003022965 *

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2014176364A (ja) * 2013-03-15 2014-09-25 Olympus Corp 幹細胞の解析方法
JP2016067311A (ja) * 2014-09-30 2016-05-09 株式会社ジーシー 軟骨分化培養液、及び軟骨組織
JP2018502556A (ja) * 2014-12-05 2018-02-01 メリディジェン・バイオテック・カンパニー・リミテッドＭｅｒｉｄｉｇｅｎ Ｂｉｏｔｅｃｈ Ｃｏ．， Ｌｔｄ． 間葉系幹細胞を区別する方法
CN110494556A (zh) * 2017-04-03 2019-11-22 株式会社钟化 包含间充质系干细胞的细胞群、其制造方法、以及医药组合物
CN110494556B (zh) * 2017-04-03 2024-04-30 株式会社钟化 包含间充质系干细胞的细胞群、其制造方法、以及医药组合物

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WO2008111161A1 (ja) 間葉系幹細胞の均質性識別方法、および、その方法を用いて単離された均質な間葉系幹細胞
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