WO2008110019A1 - Préparation d'échantillons cliniques sur une plate-forme microfluidique - Google Patents

Préparation d'échantillons cliniques sur une plate-forme microfluidique Download PDF

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Publication number
WO2008110019A1
WO2008110019A1 PCT/CA2008/000562 CA2008000562W WO2008110019A1 WO 2008110019 A1 WO2008110019 A1 WO 2008110019A1 CA 2008000562 W CA2008000562 W CA 2008000562W WO 2008110019 A1 WO2008110019 A1 WO 2008110019A1
Authority
WO
WIPO (PCT)
Prior art keywords
microfluidic
particle
beads
interest
microfluidic device
Prior art date
Application number
PCT/CA2008/000562
Other languages
English (en)
Inventor
Christopher Backhouse
Linda Pilarski
Jeeshan Chowdhury
Govind Kaigala
John Booth
Original Assignee
The Governors Of The University Of Alberta
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by The Governors Of The University Of Alberta filed Critical The Governors Of The University Of Alberta
Priority to CA002680558A priority Critical patent/CA2680558A1/fr
Publication of WO2008110019A1 publication Critical patent/WO2008110019A1/fr

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0887—Laminated structure
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/043—Moving fluids with specific forces or mechanical means specific forces magnetic forces

Definitions

  • the present invention pertains to the field of clinical sample preparation for use in microfluidic or other diagnostic devices.
  • the present invention provides for the addition of a solute to the fluid medium to Increase viscosity of said fluid medium, wherein the solute Is chosen so as not to substantially interfere with later biochemical reactions with the molecules of interest
  • the solute is sucrose of a concentration of 20-35% w/v, in a preferred embodiment the solute is 25% sucrose w/v.
  • FIGURE 1 shows a representation of the microfluidic chip (glass-glass microchip with channels etched on only one of the surface, and then irreversibly bonded to the second un- patterned glass surface) used to perform the sample purification of the present invention
  • FIGURE 2 shows a representation of the cross-sectional view of the micofiuidic channels in a microfluidic chip with "trenches"
  • the nucleic acid bound to the beads is migrated though the chip, thus effectively separating the nucleic acid from the cell debris and other introduced chemical constitutes (e.g. Protease -K) within the input chamber.
  • the migration of the beads through the controlled movement of the magnetic field maxima, performs a dual function of removing nucleic acids from cell debris and removal from cellular components which would have an adverse effect on a subsequent reaction, including but not limited to, PCR.
  • the input and output wells are filled to the same level with fluid, thus minimizing potential pressure driven flows through the chip.
  • the movement of the beads is primarily by the change in location of the magnetic maxima and not due to the bulk flow of the fluid.
  • a negative pressure gradient is established, relative to the directional flow of the magnetic beads, which may further assist in the removal of compounds having an adverse effect on later reactions or processes Alternatively, the beads may remain immobilized while a fluid flow past the beads occurs.
  • the microfluidic channels are pre-coated.
  • Bovine Serum Albumin (BSA) in distilled water (1mg/mL) is injected through the microfluidic channels and wells such that all channels are filled.
  • the solution is allowed to reside in the microfluidic device for 5 minutes, at a temperature of 18 0 C to 23 0 C.
  • the BSA solution is removed and the channels and wells are dried with pressurized air. This pre-coating significantly enhances bead movement through the microfluidic channels.

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Fluid Mechanics (AREA)
  • Dispersion Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

Le génotypage d'échantillons cliniques (par exemple, sang entier et écouvillons buccaux) pour un diagnostic moléculaire est typiquement effectué dans des laboratoires utilisant un équipement spécialisé par des opérateurs entraînés. La présente invention concerne une approche microfluidique intégrée dans laquelle une approche de lyse cellulaire, de nettoyage de molécules d'intérêt, d'isolation/purification de molécules d'intérêt et de transport de molécules d'intérêt est démontrée. Un aimant est focalisé à l'intérieur du canal microfluidique et facilite le mouvement de bille magnétique qui lie les molécules d'intérêt. L'invention concerne également un mode opératoire qui est développé pour réutiliser de façon indéfinie les puces sans dégradation notable de la performance.
PCT/CA2008/000562 2007-03-13 2008-03-13 Préparation d'échantillons cliniques sur une plate-forme microfluidique WO2008110019A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CA002680558A CA2680558A1 (fr) 2007-03-13 2008-03-13 Preparation d'echantillons cliniques sur une plate-forme microfluidique

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US90650307P 2007-03-13 2007-03-13
US60/906,503 2007-03-13

Publications (1)

Publication Number Publication Date
WO2008110019A1 true WO2008110019A1 (fr) 2008-09-18

Family

ID=39758971

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CA2008/000562 WO2008110019A1 (fr) 2007-03-13 2008-03-13 Préparation d'échantillons cliniques sur une plate-forme microfluidique

Country Status (2)

Country Link
CA (1) CA2680558A1 (fr)
WO (1) WO2008110019A1 (fr)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20120077267A1 (en) * 2010-09-28 2012-03-29 Samsung Electronics Co., Ltd. Device and method of separating cells by using magnetic force
US9062342B2 (en) 2012-03-16 2015-06-23 Stat-Diagnostica & Innovation, S.L. Test cartridge with integrated transfer module
US11219895B2 (en) 2019-07-03 2022-01-11 King Abdulaziz University Blood analysis cartridge

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2417341A1 (fr) * 2000-08-08 2002-02-14 Jing Cheng Techniques de manipulations de fragments dans des systemes microfluidiques
US6767706B2 (en) * 2000-06-05 2004-07-27 California Institute Of Technology Integrated active flux microfluidic devices and methods

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6767706B2 (en) * 2000-06-05 2004-07-27 California Institute Of Technology Integrated active flux microfluidic devices and methods
CA2417341A1 (fr) * 2000-08-08 2002-02-14 Jing Cheng Techniques de manipulations de fragments dans des systemes microfluidiques

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20120077267A1 (en) * 2010-09-28 2012-03-29 Samsung Electronics Co., Ltd. Device and method of separating cells by using magnetic force
US8993340B2 (en) * 2010-09-28 2015-03-31 Samsung Electronics Co., Ltd. Device and method of separating cells by using magnetic force
US9062342B2 (en) 2012-03-16 2015-06-23 Stat-Diagnostica & Innovation, S.L. Test cartridge with integrated transfer module
US9334528B2 (en) 2012-03-16 2016-05-10 Stat-Diagnostica & Innovation, S.L. Test cartridge with integrated transfer module
US9757725B2 (en) 2012-03-16 2017-09-12 Stat-Diagnostica & Innovation, S.L. Test cartridge with integrated transfer module
US9914119B2 (en) 2012-03-16 2018-03-13 Stat-Diagnostica & Innovation, S.L. Test cartridge with integrated transfer module
US11219895B2 (en) 2019-07-03 2022-01-11 King Abdulaziz University Blood analysis cartridge

Also Published As

Publication number Publication date
CA2680558A1 (fr) 2008-09-18

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