WO2008100424A3 - Animals and cells with genomic target sites for transposase-mediated transgenesis - Google Patents

Animals and cells with genomic target sites for transposase-mediated transgenesis Download PDF

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Publication number
WO2008100424A3
WO2008100424A3 PCT/US2008/001686 US2008001686W WO2008100424A3 WO 2008100424 A3 WO2008100424 A3 WO 2008100424A3 US 2008001686 W US2008001686 W US 2008001686W WO 2008100424 A3 WO2008100424 A3 WO 2008100424A3
Authority
WO
WIPO (PCT)
Prior art keywords
transposase
recognition
target site
animals
cells
Prior art date
Application number
PCT/US2008/001686
Other languages
French (fr)
Other versions
WO2008100424A2 (en
Inventor
Stefan Moisyadi
Joseph M. Kaminski
Pawel Pelczar
Original Assignee
University Of Hawaii
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University Of Hawaii filed Critical University Of Hawaii
Publication of WO2008100424A2 publication Critical patent/WO2008100424A2/en
Publication of WO2008100424A3 publication Critical patent/WO2008100424A3/en

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Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
    • A01K67/027—New breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
    • A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
    • A01K67/0333—Genetically modified invertebrates, e.g. transgenic, polyploid
    • A01K67/0337—Genetically modified Arthropods
    • A01K67/0339—Genetically modified insects, e.g. Drosophila melanogaster, medfly
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/105—Murine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/70—Invertebrates
    • A01K2227/706—Insects, e.g. Drosophila melanogaster, medfly
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/80—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/90—Vectors containing a transposable element

Abstract

Animals and cells with genomic target sites for transposase-mediated transgenesis are provided herein. In some embodiments, a transgenic animal containing in its genome a specific transgene target site is provided. In some embodiments, in a transgenic animal containing in its genome a specific transgene target site, the specific transgene target site can include one or more nucleotide sequence repeats that can include one or more transposon insertion sequences capable of recognition by a transposase enzyme and one or more recognition sequences for a DNA-binding polypeptide. In some embodiments, in a transgenic animal containing in its genome a specific transgene target site, the target site can include one or more nucleotide sequence repeats that include one or more transposon insertion sequences capable of recognition by a transposase enzyme and one or more recognition sequences for a DNA binding polypeptide that can be a chimeric polypeptide. In some embodiments, the recognition sequences for a DNA binding polypeptide can be recognition sequence for a transposase enzyme with one or more DNA binding domains.
PCT/US2008/001686 2007-02-09 2008-02-09 Animals and cells with genomic target sites for transposase-mediated transgenesis WO2008100424A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US90061107P 2007-02-09 2007-02-09
US60/900,611 2007-02-09

Publications (2)

Publication Number Publication Date
WO2008100424A2 WO2008100424A2 (en) 2008-08-21
WO2008100424A3 true WO2008100424A3 (en) 2010-04-08

Family

ID=39690672

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2008/001686 WO2008100424A2 (en) 2007-02-09 2008-02-09 Animals and cells with genomic target sites for transposase-mediated transgenesis

Country Status (1)

Country Link
WO (1) WO2008100424A2 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SG177247A1 (en) 2009-06-11 2012-02-28 Kek High Energy Accelerator Process for production of protein
WO2018098671A1 (en) * 2016-11-30 2018-06-07 China Agricultural University A method for crispr library screening
CN108118027B (en) * 2018-01-08 2021-12-10 内蒙古农业大学 Sheep oocyte in-vitro 'two-stage' maturation method, and pre-incubation liquid and kit thereof
CN109970871A (en) * 2019-04-28 2019-07-05 广州威生医药科技有限公司 Long-acting reorganization FSH fusion protein, preparation method and its application in sow timing semen deposition
CN115397984A (en) * 2019-11-22 2022-11-25 旗舰先锋创新Vi有限责任公司 Recombinase compositions and methods of use
KR20230005865A (en) 2020-05-04 2023-01-10 살리오젠 테라퓨틱스 인코포레이티드 potential-based therapy

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060210977A1 (en) * 2002-07-24 2006-09-21 Kaminski Joseph M Transposon-based vectors and methods of nucleic acid integration

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060210977A1 (en) * 2002-07-24 2006-09-21 Kaminski Joseph M Transposon-based vectors and methods of nucleic acid integration

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
ALFRED M. HANDLER ET AL.: "The lepidopteran transposon vector, piggyBac, mediates germ-line transformation in the Mediterranean fruit fly", PROC NATL ACAD SCI USA, vol. 95, no. 13, 23 June 1998 (1998-06-23), pages 7520 - 7525 *
ERIC T. SHINOHARA ET AL.: "Active integration: new strategies for transgenesis", TRANSGENIC RES., vol. 16, 6 March 2007 (2007-03-06), pages 333 - 339, XP019509133, DOI: doi:10.1007/s11248-007-9077-z *
K. J. MARAGATHAVALLY ET AL.: "Chimeric Mos1 and piggyBac transposases result in sitedirected integration.", THE FASEB JOURNAL., vol. 20, 20 September 2006 (2006-09-20), pages E1188 - E1195 *

Also Published As

Publication number Publication date
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