WO2008096368A3 - A novel process for production of recombinant human g-csf - Google Patents

A novel process for production of recombinant human g-csf Download PDF

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Publication number
WO2008096368A3
WO2008096368A3 PCT/IN2008/000067 IN2008000067W WO2008096368A3 WO 2008096368 A3 WO2008096368 A3 WO 2008096368A3 IN 2008000067 W IN2008000067 W IN 2008000067W WO 2008096368 A3 WO2008096368 A3 WO 2008096368A3
Authority
WO
WIPO (PCT)
Prior art keywords
csf
product yield
level
recombinant human
novel process
Prior art date
Application number
PCT/IN2008/000067
Other languages
French (fr)
Other versions
WO2008096368A2 (en
Inventor
Venkata Krishna Rao Dasari
Kali Satya Bhujanga Rao Adibhatla
Chowdary Nannapaneni Venkaiah
Original Assignee
Natco Pharma Ltd
Venkata Krishna Rao Dasari
Kali Satya Bhujanga Rao Adibhatla
Chowdary Nannapaneni Venkaiah
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Natco Pharma Ltd, Venkata Krishna Rao Dasari, Kali Satya Bhujanga Rao Adibhatla, Chowdary Nannapaneni Venkaiah filed Critical Natco Pharma Ltd
Publication of WO2008096368A2 publication Critical patent/WO2008096368A2/en
Publication of WO2008096368A3 publication Critical patent/WO2008096368A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/53—Colony-stimulating factor [CSF]
    • C07K14/535—Granulocyte CSF; Granulocyte-macrophage CSF

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biophysics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Toxicology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

The present invention relates to Recombinant human Granulocyte Colony Stimulating Factor (G-CSF) and describes a novel process for high-level expression, a simple, economically feasible, largely scalable production of rhG-CSF- from bacterial cells. The invention comprises of the development of transcriptional and translational conditions of G-CSF gene to improve the level of protein expression resulting in the increase of specific and volumetric product yield. The specific product yield in batch mode fermentation is 68mg/L/OD and the level of protein expression is 45% on total cellular protein. The volumetric product yield in fed batch mode fermentation is 4.2 g/L.
PCT/IN2008/000067 2007-02-05 2008-02-04 A novel process for production of recombinant human g-csf WO2008096368A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IN254CH2007 2007-02-05
IN254/CHE/2007 2007-02-05

Publications (2)

Publication Number Publication Date
WO2008096368A2 WO2008096368A2 (en) 2008-08-14
WO2008096368A3 true WO2008096368A3 (en) 2008-11-06

Family

ID=39682190

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IN2008/000067 WO2008096368A2 (en) 2007-02-05 2008-02-04 A novel process for production of recombinant human g-csf

Country Status (1)

Country Link
WO (1) WO2008096368A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011086447A2 (en) 2010-01-12 2011-07-21 Lupin Limited Fermentation process for the preparation of recombinant heterologous proteins
CN102154189B (en) * 2010-12-31 2015-11-11 鲁南制药集团股份有限公司 A kind of fermentation culture method of rhG-CSF recombinant bacterial strain
HUP1200171A1 (en) 2012-03-19 2013-09-30 Richter Gedeon Nyrt Methods for the production of polypeptides

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0256843A1 (en) * 1986-08-11 1988-02-24 Cetus Corporation Expression of g-csf and muteins thereof and their use
WO2004001056A1 (en) * 2002-06-24 2003-12-31 Dr. Reddy's Laboratories Ltd. Process for preparing g-csf

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0256843A1 (en) * 1986-08-11 1988-02-24 Cetus Corporation Expression of g-csf and muteins thereof and their use
WO2004001056A1 (en) * 2002-06-24 2003-12-31 Dr. Reddy's Laboratories Ltd. Process for preparing g-csf

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
"Abstract for E.coli competent cells Technical Bulletin, TB095", November 2006 (2006-11-01), XP002493701, Retrieved from the Internet <URL:www.promega.com/tbs/tb095/tb095.html> *
DEVLIN P E ET AL: "ALTERATION OF AMINO-TERMINAL CODONS OF HUMAN GRANULOCYTE-COLONY-STIMULATING FACTOR INCREASES EXPRESSION LEVELS AND ALLOWS EFFICIENT PROCESSING BY METHIONINE AMINOPEPTIDASE IN ESCHERICHIA COLI", GENE, ELSEVIER, AMSTERDAM, NL, vol. 65, no. 1, 1 January 1988 (1988-01-01), pages 13 - 22, XP001024074, ISSN: 0378-1119 *
NISHIKUBO ET AL: "Improved heterologous gene expression in Escherichia coli by optimization of the AT-content of codons immediately downstream of the initiation codon", JOURNAL OF BIOTECHNOLOGY, ELSEVIER SCIENCE PUBLISHERS, AMSTERDAM, NL, vol. 120, no. 4, 6 December 2005 (2005-12-06), pages 341 - 346, XP005168553, ISSN: 0168-1656 *
RAO DASARI V KRISHNA ET AL: "Optimization of the AT-content of codons immediately downstream of the initiation codon and evaluation of culture conditions for high-level expression of recombinant human G-CSF in Escherichia coli", MOLECULAR BIOTECHNOLOGY, vol. 38, no. 3, November 2007 (2007-11-01), pages 221 - 232, XP009104862, ISSN: 1073-6085 *
SRIVASTAVA P ET AL: "Overexpression and purification of recombinant human interferon alpha2b in Escherichia coli", PROTEIN EXPRESSION AND PURIFICATION, ACADEMIC PRESS, SAN DIEGO, CA, vol. 41, no. 2, 1 June 2005 (2005-06-01), pages 313 - 322, XP004875120, ISSN: 1046-5928 *
VOGES D ET AL: "Analyzing and enhancing mRNA translational efficiency in an Escherichia coli in vitro expression system", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, ACADEMIC PRESS INC. ORLANDO, FL, US, vol. 318, no. 2, 28 May 2004 (2004-05-28), pages 601 - 614, XP004505422, ISSN: 0006-291X *

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Publication number Publication date
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