WO2008093530A1 - 大腸癌病期検出方法 - Google Patents

大腸癌病期検出方法 Download PDF

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Publication number
WO2008093530A1
WO2008093530A1 PCT/JP2008/050327 JP2008050327W WO2008093530A1 WO 2008093530 A1 WO2008093530 A1 WO 2008093530A1 JP 2008050327 W JP2008050327 W JP 2008050327W WO 2008093530 A1 WO2008093530 A1 WO 2008093530A1
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WO
WIPO (PCT)
Prior art keywords
clinical stage
colorectal cancer
cdna
cox
detecting
Prior art date
Application number
PCT/JP2008/050327
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English (en)
French (fr)
Inventor
Shigeru Kanaoka
Original Assignee
National University Corporation, Hamamatsu University School Of Medicine
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by National University Corporation, Hamamatsu University School Of Medicine filed Critical National University Corporation, Hamamatsu University School Of Medicine
Priority to JP2008556040A priority Critical patent/JPWO2008093530A1/ja
Publication of WO2008093530A1 publication Critical patent/WO2008093530A1/ja

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/112—Disease subtyping, staging or classification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Hospice & Palliative Care (AREA)
  • Biophysics (AREA)
  • Oncology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

　大腸癌の病期の診断のためのＣＯＸ－２の検出方法、及びＣＯＸ－２を含む大腸癌の病期の診断用キットの提供。 　工程：ａ）糞便を採取し、凍結する工程、ｂ）凍結した糞便をＲＮＡ分解酵素阻害剤の存在下で均質化し、懸濁物を調製する工程、ｃ）得られたＲＮＡを抽出するための試料から、ＲＮＡを抽出する工程、ｄ）抽出されたＲＮＡを逆転写し、ｃＤＮＡを得る工程、ｅ）得られたｃＤＮＡを増幅する工程、ｆ）増幅されたｃＤＮＡを検出する工程、ｇ）検出されたｃＤＮＡ量を定量する工程及びｈ）得られたｃＤＮＡ量が大腸癌のいずれの病期に属するかを決定する工程を含む、大腸癌の病期の診断のためのＣＯＸ－２の検出方法。
PCT/JP2008/050327 2007-01-30 2008-01-15 大腸癌病期検出方法 WO2008093530A1 (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2008556040A JPWO2008093530A1 (ja) 2007-01-30 2008-01-15 大腸癌病期検出方法

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007019235 2007-01-30
JP2007-019235 2007-01-30

Publications (1)

Publication Number Publication Date
WO2008093530A1 true WO2008093530A1 (ja) 2008-08-07

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ID=39673844

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2008/050327 WO2008093530A1 (ja) 2007-01-30 2008-01-15 大腸癌病期検出方法

Country Status (2)

Country Link
JP (1) JPWO2008093530A1 (ja)
WO (1) WO2008093530A1 (ja)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11479820B2 (en) 2016-10-27 2022-10-25 Geneoscopy, Inc. Detection method using eukaryotic cells
US11479824B2 (en) 2018-06-01 2022-10-25 Geneoscopy, Inc. Detection method for cancer using RNA biomarkers

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004083856A1 (ja) * 2003-03-19 2004-09-30 Hamamatsu Foundation For Science And Technology Promotion 大腸癌マーカー検出方法

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004083856A1 (ja) * 2003-03-19 2004-09-30 Hamamatsu Foundation For Science And Technology Promotion 大腸癌マーカー検出方法

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11479820B2 (en) 2016-10-27 2022-10-25 Geneoscopy, Inc. Detection method using eukaryotic cells
US11479824B2 (en) 2018-06-01 2022-10-25 Geneoscopy, Inc. Detection method for cancer using RNA biomarkers

Also Published As

Publication number Publication date
JPWO2008093530A1 (ja) 2010-05-20

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