WO2008090290A3 - Milieu de culture synthétique chimiquement défini - Google Patents

Milieu de culture synthétique chimiquement défini Download PDF

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Publication number
WO2008090290A3
WO2008090290A3 PCT/FR2007/002099 FR2007002099W WO2008090290A3 WO 2008090290 A3 WO2008090290 A3 WO 2008090290A3 FR 2007002099 W FR2007002099 W FR 2007002099W WO 2008090290 A3 WO2008090290 A3 WO 2008090290A3
Authority
WO
WIPO (PCT)
Prior art keywords
culture medium
defined synthetic
chemically defined
synthetic culture
chemically
Prior art date
Application number
PCT/FR2007/002099
Other languages
English (en)
Other versions
WO2008090290A2 (fr
Inventor
Haridon Rene L
Original Assignee
Agronomique Inst Nat Rech
Haridon Rene L
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Agronomique Inst Nat Rech, Haridon Rene L filed Critical Agronomique Inst Nat Rech
Publication of WO2008090290A2 publication Critical patent/WO2008090290A2/fr
Publication of WO2008090290A3 publication Critical patent/WO2008090290A3/fr

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018Culture media for cell or tissue culture
    • C12N5/005Protein-free medium
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00Specific components of cell culture medium
    • C12N2500/30Organic components
    • C12N2500/32Amino acids
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00Specific components of cell culture medium
    • C12N2500/30Organic components
    • C12N2500/34Sugars
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00Specific components of cell culture medium
    • C12N2500/30Organic components
    • C12N2500/38Vitamins
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00Genetically modified cells
    • C12N2510/02Cells for production

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

L'invention concerne un milieu de culture cellulaire synthétique, chimiquement défini, de préférence sans sérum et aprotéique, une méthode de culture de cellules dans ce milieu synthétique, chimiquement défini, et une méthode de production de protéines recombinantes, en particulier d'anticorps monoclonaux, d'interféron gamma ou de virus.
PCT/FR2007/002099 2006-12-19 2007-12-18 Milieu de culture synthétique chimiquement défini WO2008090290A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR0611090 2006-12-19
FR0611090A FR2910024B1 (fr) 2006-12-19 2006-12-19 Milieu de culture synthetique chimiquement defini.

Publications (2)

Publication Number Publication Date
WO2008090290A2 WO2008090290A2 (fr) 2008-07-31
WO2008090290A3 true WO2008090290A3 (fr) 2008-09-18

Family

ID=38068823

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/FR2007/002099 WO2008090290A2 (fr) 2006-12-19 2007-12-18 Milieu de culture synthétique chimiquement défini

Country Status (2)

Country Link
FR (1) FR2910024B1 (fr)
WO (1) WO2008090290A2 (fr)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SG185037A1 (en) 2010-04-26 2012-11-29 Novartis Ag Improved cell culture medium
EP3736326B1 (fr) * 2015-04-01 2023-05-10 Boehringer Ingelheim International GmbH Milieu de culture de cellules
WO2017106783A1 (fr) * 2015-12-17 2017-06-22 Life Technologies Corporation Pastilles utilisées en culture cellulaire et leurs procédés de fabrication
GB2584472B (en) * 2019-06-05 2021-10-13 Yara Uk Ltd Chemical composition for seed treatment
CN113528601B (zh) * 2020-04-13 2023-08-04 菲鹏生物股份有限公司 一种细胞培养方法
CN113755449A (zh) * 2021-09-30 2021-12-07 南京天纵易康生物科技股份有限公司 一种提高杂交瘤细胞存活率的营养补充剂、培养基及培养方法

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0076647A2 (fr) * 1981-10-02 1983-04-13 HANA Biologics Incorporated Milieux de culture pour cellules issues du système immun

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0076647A2 (fr) * 1981-10-02 1983-04-13 HANA Biologics Incorporated Milieux de culture pour cellules issues du système immun

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
DUCOMMUN PAUL ET AL: "The role of vitamins and amino acids on hybridoma growth and monoclonal antibody production", CYTOTECHNOLOGY, vol. 37, no. 2, 2001, pages 65 - 73, XP002436584, ISSN: 0920-9069 *
GONG XIANGHUI ET AL: "Fed-batch culture optimization of a growth-associated hybridoma cell line in chemically defined protein-free media", CYTOTECHNOLOGY, vol. 52, no. 1, September 2006 (2006-09-01), pages 25 - 38, XP002436585, ISSN: 0920-9069 *

Also Published As

Publication number Publication date
WO2008090290A2 (fr) 2008-07-31
FR2910024B1 (fr) 2009-03-27
FR2910024A1 (fr) 2008-06-20

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