WO2008081457A3 - Methods of isolating cardiac stem cells, banking and uses thereof - Google Patents

Methods of isolating cardiac stem cells, banking and uses thereof Download PDF

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Publication number
WO2008081457A3
WO2008081457A3 PCT/IL2008/000026 IL2008000026W WO2008081457A3 WO 2008081457 A3 WO2008081457 A3 WO 2008081457A3 IL 2008000026 W IL2008000026 W IL 2008000026W WO 2008081457 A3 WO2008081457 A3 WO 2008081457A3
Authority
WO
WIPO (PCT)
Prior art keywords
stem cells
cardiac stem
banking
methods
isolating cardiac
Prior art date
Application number
PCT/IL2008/000026
Other languages
French (fr)
Other versions
WO2008081457A2 (en
Inventor
Ayelet Itzhaki-Alfia
Israel Barbash
Jonathan Leor
Original Assignee
Univ Ramot
Tel Hashomer Medical Res Infrastructure & Services Ltd
Ayelet Itzhaki-Alfia
Israel Barbash
Jonathan Leor
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Ramot, Tel Hashomer Medical Res Infrastructure & Services Ltd, Ayelet Itzhaki-Alfia, Israel Barbash, Jonathan Leor filed Critical Univ Ramot
Publication of WO2008081457A2 publication Critical patent/WO2008081457A2/en
Publication of WO2008081457A3 publication Critical patent/WO2008081457A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/34—Muscles; Smooth muscle cells; Heart; Cardiac stem cells; Myoblasts; Myocytes; Cardiomyocytes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0657—Cardiomyocytes; Heart cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2509/00—Methods for the dissociation of cells, e.g. specific use of enzymes

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Developmental Biology & Embryology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Rheumatology (AREA)
  • Cardiology (AREA)
  • Virology (AREA)
  • Immunology (AREA)
  • Vascular Medicine (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

A method of isolating cardiac stem cells is disclosed. The method comprises contacting a tissue which comprises the cardiac stem cells with a composition which comprises dispase II under conditions sufficient to induce cell dissociation. Banks of the isolated cardiac stem cells are also disclosed.
PCT/IL2008/000026 2007-01-04 2008-01-03 Methods of isolating cardiac stem cells, banking and uses thereof WO2008081457A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US87838907P 2007-01-04 2007-01-04
US60/878,389 2007-01-04

Publications (2)

Publication Number Publication Date
WO2008081457A2 WO2008081457A2 (en) 2008-07-10
WO2008081457A3 true WO2008081457A3 (en) 2008-12-31

Family

ID=39414935

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IL2008/000026 WO2008081457A2 (en) 2007-01-04 2008-01-03 Methods of isolating cardiac stem cells, banking and uses thereof

Country Status (1)

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WO (1) WO2008081457A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201304831D0 (en) 2013-03-15 2013-05-01 Coretherapix Slu Adult cardiac stem cell population
US9987310B2 (en) 2013-11-27 2018-06-05 University Of Louisville Research Foundation, Inc. Cardiac progenitor cells and methods of use therefor
US20150328263A1 (en) 2014-05-14 2015-11-19 University Of Maryland, Baltimore Cardiac stem cells for cardiac repair
CN114938630A (en) * 2019-11-15 2022-08-23 尼奥普罗根公司 Immortalized cardiac stem cells for cardiac repair

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999049015A2 (en) * 1998-03-23 1999-09-30 Zymogenetics, Inc. Cardiac-derived stem cells
WO2005012510A1 (en) * 2003-07-31 2005-02-10 Università Degli Studi Di Roma 'la Sapienza ' Method for the isolation and expansion of cardiac stem cells from biopsy

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999049015A2 (en) * 1998-03-23 1999-09-30 Zymogenetics, Inc. Cardiac-derived stem cells
WO2005012510A1 (en) * 2003-07-31 2005-02-10 Università Degli Studi Di Roma 'la Sapienza ' Method for the isolation and expansion of cardiac stem cells from biopsy

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
BELTRAMI ANTONIO P ET AL: "Adult cardiac stem cells are multipotent and support myocardial regeneration", CELL, CELL PRESS, CAMBRIDGE, NA, US, vol. 114, no. 6, 19 September 2003 (2003-09-19), pages 763 - 776, XP002304004, ISSN: 0092-8674 *
LAUGWITZ KARL-LUDWIG ET AL: "Postnatal isl1+ cardioblasts enter fully differentiated cardiomyocyte lineages", 20050210, vol. 433, no. 7026, 10 February 2005 (2005-02-10), pages 647 - 653, XP002477756 *
YAMADA ET AL: "Cardiac progenitor cells in brown adipose tissue repaired damaged myocardium", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, ACADEMIC PRESS INC. ORLANDO, FL, US, vol. 342, no. 2, 7 April 2006 (2006-04-07), pages 662 - 670, XP005300600, ISSN: 0006-291X *

Also Published As

Publication number Publication date
WO2008081457A2 (en) 2008-07-10

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