WO2008056325A3 - Process for animal species identification in samples with genetic material based on mitochondrial dna size variation - Google Patents

Process for animal species identification in samples with genetic material based on mitochondrial dna size variation Download PDF

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Publication number
WO2008056325A3
WO2008056325A3 PCT/IB2007/054509 IB2007054509W WO2008056325A3 WO 2008056325 A3 WO2008056325 A3 WO 2008056325A3 IB 2007054509 W IB2007054509 W IB 2007054509W WO 2008056325 A3 WO2008056325 A3 WO 2008056325A3
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WO
WIPO (PCT)
Prior art keywords
present
species identification
mitochondrial dna
animal species
dna
Prior art date
Application number
PCT/IB2007/054509
Other languages
French (fr)
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WO2008056325B1 (en
WO2008056325A2 (en
Inventor
Pereira Filipe Adao Macedo
Van Asch Barbara Joana Koehler
De Sousa Botelho De Rodrigues
Original Assignee
Univ Do Porto
Pereira Filipe Adao Macedo
Van Asch Barbara Joana Koehler
De Sousa Botelho De Rodrigues
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Univ Do Porto, Pereira Filipe Adao Macedo, Van Asch Barbara Joana Koehler, De Sousa Botelho De Rodrigues filed Critical Univ Do Porto
Publication of WO2008056325A2 publication Critical patent/WO2008056325A2/en
Publication of WO2008056325A3 publication Critical patent/WO2008056325A3/en
Publication of WO2008056325B1 publication Critical patent/WO2008056325B1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present invention refers to a process for animal species identification in biological samples with genetic material of animal origin, based on variations in the size of mitochondrial 12s and 16s ribosomal RNA gene sequences. The method of this invention comprises a panel of oligonucleotide PCR (Polymerase Chain Reaction) primers, labelled with fluorescent dyes, for multiplex PCR amplification of seven mitochondrial DNA regions with insertion\deletion polymorphisms, allowing fragment size detection and analysis by automated capillary electrophoresis or similar techniques. The species identification is achieved by comparing the target sample profile (DNA fragment sizes) with pre-established standard species-specific fragment sizes. With the present invention is possible to detect and identify animal species in highly degraded biological samples due to the analysis of mitochondrial DNA, present in many copies per cell. This invention is advantageous when high throughput sample analyses are needed such as for large-scale species identification procedures in forensics, food quality control, ancient DNA, paleogenetics and ecology studies. Another aspect of the present invention relates to a kit containing reagents needed to practice the above-referred method.
PCT/IB2007/054509 2006-11-06 2007-11-06 Process for animal species identification in samples with genetic material based on mitochondrial dna size variation WO2008056325A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
PT103599 2006-11-06
PT103599A PT103599B (en) 2006-11-06 2006-11-06 PROCESS OF IDENTIFICATION OF ANIMAL SPECIES IN SAMPLES CONTAINING GENETIC MATERIAL, BASED ON DETERMINATION OF THE SIZE OF MITOCHONDRIAL DNA SEQUENCES

Publications (3)

Publication Number Publication Date
WO2008056325A2 WO2008056325A2 (en) 2008-05-15
WO2008056325A3 true WO2008056325A3 (en) 2008-08-21
WO2008056325B1 WO2008056325B1 (en) 2008-11-13

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB2007/054509 WO2008056325A2 (en) 2006-11-06 2007-11-06 Process for animal species identification in samples with genetic material based on mitochondrial dna size variation

Country Status (2)

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PT (1) PT103599B (en)
WO (1) WO2008056325A2 (en)

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FR2975698B1 (en) * 2011-05-26 2013-05-17 Univ Grenoble 1 UNIVERSAL PRIMERS AND THEIR USE FOR DETECTION AND
FR2975699B1 (en) * 2011-05-26 2013-05-17 Univ Grenoble 1 UNIVERSAL PRIMERS AND THEIR USE FOR DETECTION AND
FR2975697B1 (en) 2011-05-26 2013-05-17 Univ Grenoble 1 UNIVERSAL PRIMERS AND THEIR USE FOR DETECTION AND
EP2636753B1 (en) * 2012-03-09 2018-07-25 City University of Hong Kong Method and means for identification of animal species
US10214780B2 (en) 2012-03-09 2019-02-26 City University Of Hong Kong Method and means for identification of animal species
ITTO20130501A1 (en) * 2013-06-18 2014-12-19 Consiglio Nazionale Ricerche PROCEDURE AND KIT FOR FOOD ANALYSIS
CN104774958B (en) * 2015-04-27 2017-06-06 山东省农业科学院生物技术研究中心 Differentiate the animal derived primer combination of probe thing of donkey, horse, fox, kit and multiple real time fluorescence quantifying PCR detection method
CN109439761A (en) * 2018-06-26 2019-03-08 中国计量大学 Application of the COI sequence in Rapid identification river Puffer and its fish product
US20210403997A1 (en) * 2018-12-18 2021-12-30 Ricoh Company, Ltd. Device, nucleic acid testing method and nucleic acid testing device, and gene testing method
CN110066878B (en) * 2019-03-20 2023-06-02 中山大学 DNA molecular identification method of earthworm medicinal material in Naoxintong preparation
CN110144406B (en) * 2019-04-12 2022-03-11 广西大学 Method for screening DNA bar codes of Kebao broiler chickens and application thereof
CN110628918A (en) * 2019-10-12 2019-12-31 北京市食品安全监控和风险评估中心（北京市食品检验所） Kit for detecting nutria-derived component in food and application thereof
CN110804664B (en) * 2019-11-26 2022-12-06 拱北海关技术中心 Primer pair and kit for identifying bark beetles in Meidiao and application of primer pair and kit
CN111118175A (en) * 2020-01-16 2020-05-08 四川省农业科学院植物保护研究所 Method for identifying citrus leaf miner by utilizing mitochondrial molecular marker
CN111690658B (en) * 2020-05-26 2023-12-22 长春海关技术中心 DNA extraction and identification method of oviductus ranae
CN112111580B (en) * 2020-09-08 2022-07-01 中国肉类食品综合研究中心 Identification method of Qingyu pig source components
CN112111579B (en) * 2020-09-08 2022-08-02 中国肉类食品综合研究中心 Identification method of Tan sheep derived components
CN112391480A (en) * 2020-11-17 2021-02-23 广东省科学院动物研究所 Mitochondrial marker-based assessment method for genetic diversity of ocellatus mystus population
CN113444814B (en) * 2021-08-17 2023-10-27 中国科学院西北高原生物研究所 Kit and method for identifying Chinese zokor
CN114317766A (en) * 2021-12-14 2022-04-12 浙江农林大学 Eurotium cristatum molecule identification method based on mitochondria CYTB gene
CN114990201B (en) * 2022-06-08 2024-05-03 东北林业大学 Feasibility assessment method based on fecal total DNA applied to different genetic analysis
CN116875702B (en) * 2023-03-07 2024-03-22 最高人民检察院检察技术信息研究中心 Primer group for identifying common animal species, kit and application thereof
CN116814809B (en) * 2023-08-04 2024-03-12 湖南农业大学 DNA PCR detection method for environment of rana spinosa

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Also Published As

Publication number Publication date
WO2008056325B1 (en) 2008-11-13
PT103599A (en) 2008-05-30
WO2008056325A2 (en) 2008-05-15
PT103599B (en) 2009-08-06

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