WO2008041460A1 - Active oxygen scavenger, activating agent and collagen production promoter each comprising cacalol - Google Patents

Active oxygen scavenger, activating agent and collagen production promoter each comprising cacalol Download PDF

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Publication number
WO2008041460A1
WO2008041460A1 PCT/JP2007/067795 JP2007067795W WO2008041460A1 WO 2008041460 A1 WO2008041460 A1 WO 2008041460A1 JP 2007067795 W JP2007067795 W JP 2007067795W WO 2008041460 A1 WO2008041460 A1 WO 2008041460A1
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Prior art keywords
extract
plant
oil
roll
acid
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PCT/JP2007/067795
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French (fr)
Japanese (ja)
Inventor
Kazutoshi Shindo
Hiroaki Kitazawa
Masaru Kitagawa
Shuhei Yamamoto
Michiko Suzuki
Atsushi Sogabe
Original Assignee
Toyo Boseki Kabushiki Kaisha
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Priority to JP2008517667A priority Critical patent/JPWO2008041460A1/en
Publication of WO2008041460A1 publication Critical patent/WO2008041460A1/en

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/335—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin
    • A61K31/34—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin having five-membered rings with one oxygen as the only ring hetero atom, e.g. isosorbide
    • A61K31/343—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin having five-membered rings with one oxygen as the only ring hetero atom, e.g. isosorbide condensed with a carbocyclic ring, e.g. coumaran, bufuralol, befunolol, clobenfurol, amiodarone
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/49—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds
    • A61K8/4973—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with oxygen as the only hetero atom
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783—Angiosperms [Magnoliophyta]
    • A61K8/9789—Magnoliopsida [dicotyledons]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P39/00—General protective or antinoxious agents
    • A61P39/06—Free radical scavengers or antioxidants
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/08—Anti-ageing preparations
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D307/00—Heterocyclic compounds containing five-membered rings having one oxygen atom as the only ring hetero atom
    • C07D307/77—Heterocyclic compounds containing five-membered rings having one oxygen atom as the only ring hetero atom ortho- or peri-condensed with carbocyclic rings or ring systems
    • C07D307/92—Naphthofurans; Hydrogenated naphthofurans

Definitions

  • the first invention of the present invention relates to a drug excellent in active oxygen scavenging action. More details
  • a second invention of the present invention relates to an activator containing a power roll or an extract of the genus Cacalia, and in particular, a power calorie that is effective in activating skin fibroblasts and preventing aging. And cosmetics, quasi-drugs, pharmaceuticals, and foods and drinks containing extracts of plants belonging to the genus Cacalia.
  • a third invention of the present invention relates to a method for producing a plant extract containing a power roll.
  • a fourth invention of the present invention relates to a collagen production promoter comprising a plant extract containing a power roll as an active ingredient.
  • Reactive oxygen species generally react with components such as adjacent lipids, nucleic acids, and proteins in a living body that has high chemical reactivity, and cause oxidative damage that leads to various diseases.
  • Reactive oxygen causes collagen fiber cross-linking, DNA damage, tissue damage due to chain radical generation, skin and living body aging, inflammation, smooth muscle damage in myocardial infarction, deterioration of liver damage, brain tissue It is known to cause dementia due to the destruction of.
  • the reactive oxygen species scavenger having the activity of efficiently eliminating these active oxygen species is a component of components contained in the living body, foods, pharmaceuticals, agricultural chemicals, etc. It is useful as a protective agent against oxidative degradation and is expected to be used practically in the food industry, especially in the marine products, health foods, and nutritional foods, as well as in the fields of pharmaceuticals, agricultural chemicals, and cosmetics.
  • vitamins C, E, carotenoids and natural products such as dibutylhydroxytoluene, butylhydroxybisole and synthetic compounds are effective.
  • these substances only erase one or two reactive oxygen species, their active oxygen scavenging activity is not sufficient.
  • antioxidants have a problem in stability when blended with cosmetics or the like having low stability, and have poor practicality.
  • the power roll is one of the typical maple extract components, and 5,6,7,8-tetrahydrone-3,4,5-trimethylnaphtho [2,3-b] furan-9 -It is a non-polyphenolic fat-soluble substance with an all structure.
  • power rolls are also contained in extracts from plants other than Momijigasa. For example, Cacalia
  • Non-patent document 5 Adenostyloides (Non-patent document 5), Cacalia auriculata, Non-patent document 6), Ligularia virgaur ea (Non-patent document 7), Psacalium radulifolium (Non-patent document 3) and the like have been reported.
  • Non-patent Document 1 Power rolls are known to have DPPH (1, l-Diphenyl-2-picrylhydrazyl) scavenging ability (Non-patent Document 1), lipid peroxide inhibiting ability (Non-patent Document 2), etc. .
  • Non-patent Document 3 antibacterial action
  • Non-Patent Document 4 hypoglycemic action
  • the superoxide, hydrogen peroxide, and the scavenging action against active oxygen species such as singlet oxygen and the stability generated in the living body were not known at all.
  • the skin is composed of epidermal cells, fibroblasts, and extracellular matrices such as elastin and collagen that support these extracellular skin structures.
  • extracellular matrices such as elastin and collagen that support these extracellular skin structures.
  • moisture retention, flexibility, and elasticity are ensured by maintaining the homeostasis of the interaction between these skin tissues, and the skin has a firmness and wrinkles in appearance, and is maintained in a fresh state.
  • the extracellular matrix and fibroblast function are particularly weakened.
  • the skin's flexibility and moisturizing function are reduced, and the skin loses its habit and becomes rough. Aging symptoms such as wrinkles and dullness occur.
  • plant-derived activators include sesame seeds, peonies, chili peppers, toki, dokudami, bakumondo (Patent Document 4), almonds, dandelion, instantonito, sensyuyu, assembly, sohakhi, tonin, carrot, hop, mukuge, Extracts of gyokuinin (patent document 5), ginger family turmeric genus (patent document 6), and blossom genus Hanayasuri (patent document 7) are known. Some of these are used in quasi-drugs and cosmetics as activators and anti-aging agents, but no activators that exhibit satisfactory effects have been obtained.
  • Non-patent document 7 Psacalium radulifolium (Non-patent document 3) and the like have been reported.
  • Momijigasa (Cacalia delphiniifolia Sieb et Zucc) is also known as Sidke, Sidki, Sidkina, Shindoki, Kinoshita, Toukichi, Tokichina, Sudoke, Sitgi, Sudoke, and is widely distributed in Japan. It is eaten as a wild vegetable. Momijigasa extract is known to have a whitening effect (Patent Document 8).
  • One of the main components of Momijigasa is force carol.
  • Non-patent Document 2 It is known that a powerful roll has excellent antioxidant ability that suppresses lipid peroxidation (Non-patent Document 2). As the physiological action of force rolls, antibacterial action (Non-patent Document 3) and hypoglycemic action (Non-Patent Document 4) have been reported. However, the activation effect of the force roll on cells and the anti-aging effect were not known at all.
  • the skin is composed of epidermal cells, fibroblasts, and extracellular matrices such as elastin and collagen that support these extracellular skin structures.
  • extracellular matrices such as elastin and collagen that support these extracellular skin structures.
  • the interaction of these skin tissues maintains homeostasis, ensuring moisture retention, flexibility, and elasticity, and the skin is maintained in a fresh state with tension and wrinkles. It is.
  • aging, UV rays, dryness, stress, etc. cause a decrease in the function of the extracellular matrix and fibroblasts in particular, resulting in a decrease in skin flexibility and moisturizing function, and the skin loses its habits. Aging symptoms such as roughening, wrinkles and dullness occur.
  • plant-derived activators examples include sesame seeds, peonies, capsicum, toki, dokudami, batamando (patent document 4), almonds, dandelion, instantonitoko, senkyu, assembly, sohakhi, tonin, carrot, hop, mukuge, Extracts of gyokuinin (patent document 5), ginger family turmeric genus (patent document 6), and genus genus genus (patent document 7) are known.
  • plant secondary metabolites include essential oils, taste components, And alkaloids are known.
  • the “power roll” as used in the present invention is one of typical maple extract components, and includes 5, 6, 7, 8-tetrahydro 3, 4, 5-trimethyl naphtho [2, 3— b] Non-polyphenolic (non-flavonoid) plant secondary metabolite with furan 9-ol structure.
  • power rolls are also contained in extracts from plants other than Momijigasa.
  • Cacalia adenostyloides Non-Patent Document 5
  • Cacalia auriculata Non-Patent Document 6
  • Non-patent document 7 Psacalium radulifolium (Non-patent document 3) and the like have been reported. (Additional quotation from Invention 2)
  • Momijigasa is also known as Sidke, Sidki, Sidkina, Shindoki, Kinoshita, Toukichi, Tokichina, Sudoke, Sitogi, Sudoke, and is widely distributed in the country and is eaten as a wild vegetable. Yes.
  • a whitening effect is known for the extract of Japanese maple (Patent Document 8).
  • a powerful roll exists as one of the main components of the extract of Momijigasa, and it is known that the powerful roll has an excellent antioxidant ability to suppress lipid peroxidation! /, Ru (non-patent document 2).
  • As physiological effects of force rolls antibacterial action (Non-patent Document 3) and hypoglycemic action (Non-Patent Document 4) have been reported.
  • an extract obtained by extracting a plant with an organic solvent or the like often contains a large amount of chlorophyll, which is a pigment unique to photosynthetic organisms. Chlorophyll is readily converted chemically and enzymatically into degradation products such as pheophorbide. Since this chlorophyll degradation product is one of the substances causing photosensitivity that causes inflammation of the skin and the like by sunlight, it is necessary to remove chlorophyll and its degradation products from the extract (Non-patent Document 8). In addition, plant extracts that contain a lot of black-filled pigments are more susceptible to deterioration during storage due to sunlight, fluorescent light, etc. It was an important technology to remove the power as much as possible, more economically. However, the known Japanese maple extract is simply extracted with an organic solvent such as methanol and then concentrated under reduced pressure (Patent Document 8). There is no method for reducing the chlorophyll concentration and increasing the power roll concentration. It has never been known before.
  • a substance having an extracellular matrix production promoting action for example, a licorice leaf extract (patent document 9), a lotus germ extract (patent text) having an action of promoting collagen production. 10), hydrolyzed potato protein (Patent Document 11), bamboo leaf extract or hawthorn fruit extract (Patent Document 12), etc., some of which are used as a collagen production promoter in the pharmaceutical department.
  • Patent Document 9 a licorice leaf extract
  • a lotus germ extract having an action of promoting collagen production. 10
  • Patent Document 11 hydrolyzed potato protein
  • Patent Document 12 bamboo leaf extract or hawthorn fruit extract
  • Patent Document 1 Japanese Patent Laid-Open No. 62-84024
  • Patent Document 2 JP-A 63-188697
  • Patent Document 3 Japanese Patent Laid-Open No. 03-141299
  • Patent Document 4 Japanese Patent Laid-Open No. 10-45615
  • Patent Document 5 Japanese Patent Laid-Open No. 10-36279
  • Patent Document 6 Japanese Patent Publication No. 2004-75632
  • Patent Document 7 JP-A-2005-89375
  • Patent Document 8 Japanese Unexamined Patent Publication No. 05-345705
  • Patent Document 9 Japanese Unexamined Patent Publication No. 2000-191498
  • Patent Document 10 Japanese Unexamined Patent Application Publication No. 2002-29980
  • Patent Document 11 Japanese Unexamined Patent Publication No. 2005-263689
  • Patent Document 12 Japanese Unexamined Patent Publication No. 2006-160629
  • Non-patent literature l Revista de la sociedad Quimica de Mexico., 47, 160 (2003)
  • Non-patent literature 2 Bioscience Biotechnology and Biochemistry, 68, 1393 (2004)
  • Non-patent literature 3 Journal of Natural Products, 64, 432 ( 2001)
  • Non-Patent Document 4 Journal of Natural Products, 62, 1088 (1999)
  • Non-Patent Document 5 Chemical & Pharmaceutical Bulletin, 33, 4792 (1985)
  • Non-Patent Document 6 Khimiya Prirodnykh Soedienli, 5, 643 (1989)
  • Non-Patent Document 7 Phytochemistry, 30, 3132 (1991)
  • Non-Patent Document 8 Food & Food Ingredients J. Jpn, 172, 29 (1997)
  • FIG. 5 is a diagram showing the amount of power rolls in the precipitate when the water content of ethanol is changed.
  • FIG. 6 is a diagram showing the amount of power roll in the supernatant when the water content of ethanol is changed.
  • FIG. 7 shows a production scheme of Example 2.
  • FIG. 8 shows the results of confirming the collagen production promoting action on normal human skin fibroblasts by a plant extract containing a power roll.
  • the first invention of the present invention is to solve the conventional problems, to provide an active oxygen scavenger excellent in active oxygen scavenging action, and to provide a highly stable external preparation.
  • the second invention of the present invention is as follows.
  • the purpose of the second invention of the present invention is to provide an activator and an anti-aging agent that are excellent in activation and anti-aging effects on cells, and have safety sufficient to withstand long-term use. To provide cosmetics and quasi-drugs, pharmaceuticals, and foods and drinks.
  • Patent Document 8 the known Japanese maple extract is simply extracted with an organic solvent such as methanol and concentrated under reduced pressure. The concentration of chlorophyll is lowered to increase the strength roll concentration. This method has never been known.
  • the conventional collagen production promoting substance cannot be said to have a sufficient effect, and a new collagen production promoting substance that exhibits a satisfactory effect has been strongly desired.
  • the present invention has been made in view of the above-mentioned problems, and its purpose is to remove chlorophyll from a plant extract obtained from a plant containing a power roll and to increase the power roll concentration. Another object is to provide a method for producing a plant extract, and to provide a collagen production promoter that has a remarkable collagen production promoting action and at the same time has sufficient safety to withstand long-term use.
  • the present inventors have found that the power roll has an erasing action on any active oxygen species such as superoxide, hydrogen peroxide, and singlet oxygen. And having found that the power roll has excellent stability, the first invention of the present invention has been completed. That is, the first invention of the present invention has the following configuration.
  • the active oxygen scavenger characterized by eliminating at least one active oxygen selected from the group consisting of superoxide, hydrogen peroxide, and singlet oxygen.
  • a reactive oxygen scavenger characterized by being a power carol extracted from a plant of the genus Cacalia of (1 1) or (1 2).
  • a reactive oxygen scavenging method comprising scavenging at least one active oxygen selected from the group consisting of superoxide, hydrogen peroxide, and singlet oxygen.
  • (1-11) contains a power roll as an active ingredient
  • a reactive oxygen scavenging method comprising scavenging at least two kinds of active oxygen selected from the group consisting of superoxide, hydrogen peroxide, and singlet oxygen.
  • An active oxygen scavenging method characterized by scavenging superoxide, hydrogen peroxide, and singlet oxygen.
  • the present inventors have found that a maple extract is effective in activating cells, and have completed the second invention of the present invention. . That is, the second invention of the present invention includes the following inventions.
  • the asteraceae plant is a plant belonging to the genus Batrizo (2-1) or (
  • any of the above (2— ;!) to (2-5), the activator according to 1 or the anti-aging agent according to (2-6) A pharmaceutical product characterized by containing.
  • any one of the above (2— ;!) to (2-5), the activator according to item 1, or the anti-aging agent according to (2-6) A food or drink characterized by containing.
  • the present inventors have obtained a secondary extract obtained from a plant extract containing a power roll using an appropriate solvent, which contains the original power roll. It was found that the chlorophyll content was lower than that of the plant extract (third invention), and further, the plant extract containing strong calorie was found to have a collagen production promoting action (fourth invention).
  • the present inventors have completed the third and fourth inventions of the present invention. That is, the third and fourth inventions of the present invention include the following inventions.
  • the third invention of the present invention includes the following inventions.
  • the method includes a step of obtaining a secondary extract from a plant extract containing a power roll using a mixture of water and a hydrophilic organic solvent, and the secondary extract includes the power roll.
  • (3- 2) The plant extract containing a power roll according to (3-1), characterized in that the above steps are repeated until the content of chlorophyll in the secondary extract is below the desired number. Manufacturing method.
  • the hydrophilic organic solvent is selected from the group consisting of ethanol, acetone, isopropyl alcohol, propylene glycol, butylene glycol, and butanol.
  • the mixture of water and a hydrophilic organic solvent used in the step of obtaining the secondary extract is hydrous ethanol having an ethanol content of 45% or more (3
  • the mixture of water and a hydrophilic organic solvent used in the step of removing impurities is hydrous ethanol having an ethanol content of 40% or less.
  • the fourth invention of the present invention includes the following inventions.
  • a cosmetic comprising the collagen production promoter according to (4 10) or (4 11) as an active ingredient.
  • the first invention, the second invention, and the fourth invention have the following configurations.
  • a reactive oxygen scavenger characterized by being a force roll extracted from the power of the plant of the genus Cacalia belonging to the genus Calalia, (5-1) or (5-2).
  • the active oxygen scavenger which is the force Karolka S described in (5-1) or (5-2), a force roll produced by chemical synthesis.
  • a reactive oxygen scavenging method comprising scavenging at least one active oxygen selected from the group consisting of superoxide, hydrogen peroxide, and singlet oxygen.
  • An activator comprising an extract of a genus Cacalia as an active ingredient.
  • the extract of the genus Cacalia contains a force roll (5-1) 1) Activator.
  • a collagen production promoter characterized by being a power carol extracted from a plant of the genus Cacalia belonging to the power carolka described in (5-14).
  • a collagen production promoter characterized by being the force Karolka S described in (5-14), a force roll produced by chemical synthesis.
  • the first invention of the present invention it is possible to provide a highly stable active oxygen scavenger that exerts an effect on a plurality of active oxygen species of superoxide, hydrogen peroxide, and singlet oxygen. s can.
  • active oxygen scavenger of the first invention of the present invention other active oxygen scavengers show scavenging ability only for the active oxygen species in the part, whereas all of these three active oxygen species On the other hand, it has an erasing action. Normally, when a plurality of active oxygen species generated in a living body are used for erasing, it is necessary to use a combination of active oxygen scavengers capable of erasing each.
  • the power roll of the first invention of the present invention can be expected to obtain an effective effect only with the power roll, since it can erase a plurality of active oxygen species.
  • Use of the active oxygen scavenger for the human body and other foods and drinks, pharmaceuticals, cosmetics, whitening agents, fertilizers, feeds, skin external preparations, etc. provides an excellent antioxidant effect, particularly an improvement effect on the storage stability of the product. Antioxidant effect and various disease preventive effects can be obtained.
  • An extract of the Asteraceae plant used as an active ingredient of the activator according to the second invention of the present invention has a significant activation effect on skin fibroblasts. Therefore, the activator according to the second invention of the present invention exhibits an excellent effect as an anti-aging agent. In particular, plants that are eaten as wild plants among the plants belonging to the genus Morris are excellent in safety, and the activator according to the second invention of the present invention is sufficiently resistant to long-term use! / Have an effect
  • a plant extract containing a power roll having a low chlorophyll content can be produced at low cost without performing a complicated operation.
  • the collagen production promoter according to the fourth invention of the present invention is excellent in the effect of promoting collagen production and contains a safe power roll as an active ingredient. Therefore, it is effective when it can withstand long-term use!
  • the genus Cacalia of the present invention refers to a plant belonging to the genus Compositae.
  • the genus Cacalia is
  • the power roll refers to 5,6,7,8-tetrahydride-3,4,5-trimethylnaphtho [2,3-b] furan-9-ol It is a non-polyphenol compound having a structure.
  • the power roll of the present invention is a compound represented by the formula (I).
  • the power roll of the formula (I) that can be used in the first invention of the present invention is a natural product and its derivative, and a chemical compound as long as it meets the purpose of the first invention of the present invention. Either of them may be used. In particular, natural products and their derivatives can be conveniently used for the purposes of the present invention.
  • active oxygen examples include superoxide ( ⁇ ⁇ ichi), hydroxy radical ( ⁇ ⁇ ), hydrope
  • radical species LEO '
  • radical species such as nitric oxide (NO)
  • hydrogen peroxide H 2 O 2
  • Non-radical species such as OOH), hypochlorous acid (HOC1) and ozone (O 2).
  • the power roll has high! /, Active oxygen relative to a plurality of reactive oxygen species of superoxide, hydrogen peroxide, and singlet oxygen.
  • the present inventors have found an scavenging effect and nevertheless have provided an active oxygen scavenger having high stability.
  • a power roll has DPPH elimination ability, lipid peroxide inhibition ability and the like.
  • active oxygen species such as superoxide, hydrogen peroxide, and singlet oxygen that are actually generated in the living body. .
  • antioxidants have a problem in stability when blended with cosmetics or the like having low stability, and are not practical.
  • an active oxygen scavenger having a high active oxygen scavenging effect for a plurality of active oxygen species and having high stability can be provided.
  • the plant for obtaining the power roll of the first invention of the present invention is not particularly limited.
  • Asteraceae is preferable.
  • the Asteraceae plants include the genus Dendranthema, Aster, Calend dula, Callistephus, Carthamus, Cosmos, and Dahlia. ), Gazania, Gerbera, Helianth us, Senecio, Zinnia, Achillea, Ageratum, Ammobium, Daisies (Bellis), Centa urea, Cirsium, Coreopsis, Dimorp hotheca, Echinops, Felicia, Guilaldia (Gaillar dia), Miyako genus (Gymnaster), wheat genus (Helichrysum), Rhodanse genus (Heli pterum), riatris (Liatris), Rudbeckia, Stokesia, Tagetes, bats Powers such as (Cacalia), Miricacalia, and Syneilesis. Among them, the bat power S is desirable.
  • the plants of the genus Komorisou include bat Morisou, Momijigasa, Nikkomori, Tamabuki, Innuduna, Izkanikomori, Okanikomori, Taimingasa, Kushikomorisou, Tebacomomi Jigasa, Nishinoyama Taimingasa, Mikamimori Etc.
  • Momijigasa which is preferred by edible plants, is suitable.
  • Momijigasa is also known as Sidke, Sidoki, Sidkina, Shindoki, Kinoshita, Toukichi, Tokichina, Sudoke, Sitgi, Sudoke, and is widely distributed in the family Asteraceae. It is eaten as a wild vegetable.
  • the plant tissue for obtaining the power roll is not particularly limited, but can be obtained from the whole plant or a partial tissue.
  • Sites that can be obtained are not particularly limited, but include whole trees, flowers, buds, ovary, fruits, leaves, cotyledons, stems, buds, roots, seeds, dried seeds, embryos, germs, roots and the like.
  • the plant may be extracted from a raw state, a frozen state, or a lyophilized state.
  • the extraction operation is preferably performed from a freeze-dried state.
  • it may be crushed first.
  • a mixer, a homogenizer, a mortar or the like may be used.
  • the freeze-dried product may be crushed into powder after the freeze-drying operation.
  • the crushed plant body is extracted using a solvent.
  • Extraction methods include extraction by impregnation at room temperature, in a cooled or heated state, extraction using a distillation method such as steam distillation, compression method to obtain an extract by pressing, supercritical fluid or subcritical fluid
  • a distillation method such as steam distillation
  • compression method to obtain an extract by pressing
  • supercritical fluid or subcritical fluid may be used alone or in combination of two or more.
  • 5 to 100 ml of solvent is used for the finely cut raw material lg for 1 hour to 1 month, preferably 1 to 5 days, at room temperature or under heating. It is desirable.
  • the solvent used for the extraction is not particularly limited as long as the active ingredient is effectively extracted, but is water or an organic solvent miscible with water such as a lower alcohol such as ethanol, Alternatively, a mixed solution thereof, or an organic solvent immiscible with water, such as ethyl acetate, may be used.
  • Examples include monohydric alcohols such as dichloromethane, methanolol, isopropanol, isobutanol, n-hexanol, methinoreaminoreanolol, 2-ethynolebutanol, n-octyl alcohol, Glycerin, ethylene glycolate, ethylene glycol monomethino ethenore, propylene glycol nore, propylene glycol mono methino ethenore, propylene glycol nole mono chineno ethenore, triethylene glycol, 1,3-butylene glycol, Polyhydric alcohols such as hexylene glycol or derivatives thereof, ketones such as acetone, methyl ethyl ketone, methyl isobutyl ketone, methyl-n-propyl ketone, esters such as isopropyl acetate, ethyl ether, isopropyl ether , Ether
  • Examples include animal fats such as vegetable oils, beef tallow, pork tallow and whale oil.
  • a polar solvent to which inorganic salts such as phosphate buffered saline are added and a solvent to which a surfactant is added can be used, and there is no particular limitation.
  • extraction with either water and / or alcohol is particularly preferable.
  • the extraction operation may be performed in stages. For example, extraction is performed with an organic solvent that is miscible with water, such as a lower alcohol such as ethanol, and the mixture is concentrated with an evaporator. May be separated.
  • an organic solvent that is miscible with water such as a lower alcohol such as ethanol
  • the mixture is concentrated with an evaporator. May be separated.
  • components having a tyrosinase inhibitory action, a melanin production inhibitory action, and an antioxidant action are extracted in both the organic layer and the aqueous layer, and may be used for different purposes.
  • the components of the water layer can be expected to be used when an anti-oxidant that has an affinity for water is desired, such as facial cleansing applications, skin lotion applications, and use applications.
  • the force roll obtained in this way may be used as it is, as long as it does not lose its effect.
  • the obtained extract in order to increase the concentration of the force roll, which is an active ingredient in the extract, the obtained extract can be further concentrated, liquid-liquid distribution, adsorption chromatography, normal phase or reverse phase chromatography, etc., if desired. It is also possible to attach to the means.
  • the plant from which a powerful roll is obtained may be a processed plant product.
  • the processing methods include impregnation method, distillation method, squeezing method, ultrasonic method, supercritical fluid method using water, organic solvent, water and organic solvent mixture at low temperature, room temperature and warm conditions. Extract the extract using the subcritical fluid method. Furthermore, it includes processed products such as fermenting plants and extracts collected from plants. Examples include plant extracts and plant extracts.
  • a method such as 65, 319 (2005) can be exemplified.
  • a power roll obtained by chemical synthesis can also be suitably used as an active ingredient of the active oxygen scavenger according to the present invention.
  • the use form of the power roll according to the first invention of the present invention is arbitrary, for example, a refined product with an increased content of the power roll, which is an active ingredient, or an active ingredient as a crude extract. Some power rolls can be used as pure products.
  • the power roll according to the first invention of the present invention is added as an active oxygen scavenger to cosmetics, foods and drinks, pharmaceuticals, etc.
  • the effect expected by the compound is effective.
  • the total amount is from 0.0000000001 to 1%, preferably ⁇ 0.0001% to 0.01%.
  • the active oxygen scavenger in the first invention of the present invention includes, in addition to the essential power roll of the present invention, bases and additives that are usually used in cosmetics as shown below, for example, Oils and fats, waxes, mineral oils, fatty acids, alcohols, esters, metal soap, gum And water-soluble polymer compounds, surfactants, vitamins, amino acids, whitening agents, moisturizers, microbial culture metabolites, hydroxyacetic acid, inorganic pigments, UV absorbers, astringents, antibacterial agents, bactericides, disinfectants, hair It can be used in combination with agents, fragrances, pigments / colorants, sweeteners, nutrition enhancers, hair restorers, animal and plant extracts, antioxidants, anti-inflammatory agents and the like.
  • bases and additives that are usually used in cosmetics as shown below, for example, Oils and fats, waxes, mineral oils, fatty acids, alcohols, esters, metal soap, gum And water-soluble polymer compounds, surfactants, vitamins,
  • the fats and oils include apogad oil, almond oil, wikiyo oil, sesame oil, olive oil, orange oil, orange rafa oil, sesame oil, kaka-age ⁇ , power mitsule oil, power lot oil, cucumber oil, Beef tallow, beef tallow fatty acid, cucumber nut oil, safflower oil, soybean oil, camellia oil, corn oil, rapeseed oil, persic oil, castor oil, cottonseed oil, peanut oil, turtle oil, mink oil, egg yolk oil, cocoa butter, palm oil , Palm kernel oil, owl, coconut oil, beef tallow, lard, hydrogenated oil, hydrogenated castor oil.
  • waxes examples include beeswax, carnauba wax, whale wax, lanolin, liquid lanolin, reduced lanolin, hard lanolin, candelillaro, montan wax, shellac wax and the like.
  • mineral oils examples include liquid paraffin, petrolatum, paraffin, ozokelide, ceresin, microcrystalline wax, polyethylene powder, squalene, squalane, and pristane.
  • fatty acids include lauric acid, myristic acid, normitic acid, stearic acid, behenic acid, oleic acid, 12-hydroxystearic acid, undecylenic acid, tall oil, lanolin fatty acid, isononanoic acid, caproic acid
  • synthetic fatty acids such as 2-ethylbutanoic acid, isopentanoic acid, 2-methylpentanoic acid, 2-ethylhexanoic acid and isopentanoic acid.
  • alcohols include natural alcohols such as ethanol, isopropanol, laurino-leanolone, cetanol, stearino-leno-conole, oleino-leano-reconole, lanolinano-reconole, cholesterol, and phytosterol.
  • natural alcohols such as ethanol, isopropanol, laurino-leanolone, cetanol, stearino-leno-conole, oleino-leano-reconole, lanolinano-reconole, cholesterol, and phytosterol.
  • Synthetic alcohols such as 2-hexyl decanol, isostearyl decanol, 2-octyldodecanol, ethylene oxide, ethylene glycol, diethylene glycol, triethylene glycol, ethylene glycol monoethyl ether, ethylene glycol monobutyl ether , Diethylene glycol monomethylenoate, diethylene glycol monoethylenoate, polyethylene glycol, propylene oxide, propylene glycol, polypro Pyrene glycol, 1,3-butylenegri
  • Examples include cornore, glycerin, chinenoreanoleconore, pentaerythritol, sonorebitonore, mannitol, glucose, sucrose and other polyhydric alcohols.
  • Esters include isopropyl myristate, isopropyl palmitate, butyl stearate, hexyl laurate, myristyl myristate, oleyl oleate, decyl oleate, octyldodecyl myristate, hexyl decyl dimethyloctanoate, lactic acid
  • Examples include cetyl, myristyl lactate, jetyl phthalate, dibutyl phthalate, lanolin acetate, ethylene glycol monostearate, propylene glycol monostearate, and propylene glycol dioleate.
  • Examples of the metal soap include aluminum stearate, magnesium stearate, zinc stearate, calcium stearate, zinc palmitate, magnesium myristate, zinc laurate, and zinc undecylenate.
  • gums and water-soluble polymer compounds include gum arabic, benzoin rubber, dammar gum, guayata fat, Irish moss, kala gum, tragacanth gum, kilob gum, quinseed, agar, casein, dextrin, gelatin, pectin, Starch, carrageen, chitosan salt, sulfated chitin, phosphorylated chitin, alginic acid and its salt, hyaluronic acid and its salt, chondroitin sulfate, heparin, ethyl cellulose, methylcellulose
  • Rosenorelose crystalline senorelose, polyvinylenorenoleconole, polyvinino methinoreatenore, polybulol pyrrolidone, polybull metaaterylate, polyacrylic acid salt, polyalkylene oxide such as polyethylene oxide and polypropylene oxide or cross-linking thereof Polymers, carboxybule polymers, polyethyleneimine and the like can be mentioned.
  • Surfactants include anionic surfactants (carboxylates, sulfonates, sulfate esters, phosphate esters), cationic surfactants (ammine salts, quaternary ammonium salts), amphoteric surfactants.
  • Agents carboxylic acid type amphoteric surfactant, sulfate ester type amphoteric surfactant, sulfonic acid type amphoteric surfactant, phosphate ester type amphoteric surfactant), nonionic surfactant (ether type nonionic surfactant, Ether ester type nonionic surfactant , Ester type nonionic surfactant, block polymer type nonionic surfactant, nitrogen-containing type nonionic surfactant), other surfactants (natural surfactants, derivatives of protein hydrolysates, polymer surfactants) Agents, surfactants containing titanium 'key, fluorocarbon surfactants, and the like.
  • Vitamins include retinol, retinal (vitamin A1), dehydroretinal (vitamin A2), carotene, lycopene (provitamin A), and thiamin hydrochloride and thiamine sulfate (vitamin B group).
  • Vitamin B1 riboflavin (vitamin B2), pyridoxine (vitamin B6), cyanocobalamin (vitamin B12), folic acid, nicotinic acids, pantothenic acids, biotins, choline, inositols, ascorbic acid in the vitamin C group And its derivatives, in vitamin D group, ergocalciferol (vitamin D2), cholecalciferol (vitamin D3), dihydrotachysterol, in vitamin E group, tocopherol and its derivatives, ubiquinones, in vitamin K group , Huitnadione (vitamin K1), Menaquinone (vitamin K2), Me Dione (vitamin K3), and the like menadiol (vitamin K4).
  • Amino acids include Norin, leucine, isoleucine, threonine, methionine, phenylalanine, tryptophan, lysine, glycine, alanine, asparagine, glutamine, serine, cystine, cystine, tyrosine, proline, hydroxyproline, asparagine
  • acids glutamic acid, hydroxylysine, arginine, ornithine, histidine, and their amino acid derivatives such as sulfate, phosphate, nitrate, citrate, and pyrrolidone carboxylic acid.
  • a whitening agent L-ascorbic acid and its derivatives, hydroquinone and its derivatives, tranexamic acid, lucinol, edetic acid and its derivatives, and placenta extract, t-AMC HA, acerola extract, age extract, ellagic acid or its derivatives, Flaming extract, chamomile extract, force bud flower extract (urea), kiwi extract, glutathione, tocotrienol, ferulic acid, raspberry ketone, noresinol, walurshkis, pyridoxine dipalmitate, io, kojic acid or its derivatives, darcosamine or Derivatives thereof, hydroxy cinnoic acid or derivatives thereof, dartathione, arginium extract, gonon extract, sohakhi extract, psycho extract, boufu extract, garlic mushroom mycelium culture or extract thereof, linden extract, Peach leaf extract, age extract, ku
  • Moisturizers include sodium pyridonecarboxylate, glycol, glycerin, glucose, maltose , Maltitol, sucrose, fructose, xylitonor, sonorebitoren, manoletotriose, threitol, erythritole, amylolytic sugar-reduced alcohol, sonolebithonole, and polyhydric alcohols include ethylene glycol, 1,4-butylene glycol, diglycerin, triglycerin , Tetraglycerin, 1,3-butylene glycol, propylene glycol, dipropylene glycol, polyethylene glycol, 1,3-propanedio , Serine, glycine, threonine, alanine, collagen, hydrolyzed collagen, hydronectin, fibronectin, keratin, elastin, royal jelly, chondroitin sulfate heparin, glyce oral phospholipid,
  • microbial culture metabolites include yeast extract, zinc-containing yeast extract, germanium-containing yeast extract, selenium-containing yeast extract, magnesium-containing yeast extract, rice fermentation extract, Euglena extract, and skim milk powder.
  • yeast extract zinc-containing yeast extract
  • germanium-containing yeast extract germanium-containing yeast extract
  • selenium-containing yeast extract magnesium-containing yeast extract
  • rice fermentation extract Euglena extract
  • skim milk powder examples include lactic acid fermented products.
  • Examples of the ⁇ -hydroxy acid include glyconolic acid, citrate, malic acid, tartaric acid, and lactic acid.
  • anhydrous caustic acid magnesium silicate, talc, kaolin, bentonite, my strength, titanium mica, bismuth oxychloride, zirconium oxide, magnesium oxide, zinc oxide, titanium oxide, calcium carbonate
  • magnesium carbonate yellow iron oxide, red iron oxide, black iron oxide, gundiio, chromium oxide, chromium hydroxide, carbon black, calamine and the like.
  • an ultraviolet absorber ⁇ -aminobenzoic acid derivative, salicylic acid derivative, anthranilic acid derivative, coumarin derivative, amino acid compound, benzotriazole derivative, tetrazole derivative, imidazoline derivative, pyrimidine derivative, dioxane derivative
  • examples include camphor derivatives, furan derivatives, pyrone derivatives, nucleic acid derivatives, allantoin derivatives, nicotinic acid derivatives, vitamin B6 derivatives, oxybenzone, benzophenone, guaiazurene, shikonin, baicalin, noikalein, and berberine.
  • lactic acid, tartaric acid, succinic acid, succinic acid, allantoin, zinc chloride, zinc sulfate, zinc oxide, calamine, zinc p-phenol sulfonate, potassium aluminum sulfate, resorcin, secondary chloride examples thereof include iron and tannic acid.
  • ovata extract As an antibacterial agent, ovata extract, halocarban, chlorophenesine, lysozyme chloride, alkyldiaminoethylglycine hydrochloride, isopropylmethylphenol, thymol, hexacyclophene, berberine, thixolone, salicylic acid and their derivatives, Benzoic acid, sodium benzoate, paraoxybenzoic acid ester, parachloromethcresol, benzalkonium chloride, phenoxyethanol, isopropylmethylphenol, carboxylic acid, sorbic acid, potassium sorbate, hexaclonal fenphene, chlorhexidine chloride, trichloro Oral carbanilide, photosensitizer, bis (2-pyridinorethio 1-oxide) zinc, thianthol, hinokitiol, triclosan, trichlorohydroxydiphenenoleethenole,
  • a disinfectant for example, as a disinfectant, atalinol, io, benzalkonium chloride, benzethonium chloride, methyl rosaniline chloride, cresol monole, calcium dalconate, chlorhexidine dalconate, sulfamine, mercurochrome, latatoferrin or its addition Moisture breakdown products.
  • a hair preparation selenium disulfide, alkylisoquinolinium bromide, zinc pilichn, bifuenamine, thianthol, castari tincture, pepper tincture, pepper tincture, quinine hydrochloride, strong ammonia water
  • examples include potassium bromate, sodium bromate, and thiodaricholic acid.
  • fragrance natural animal fragrances such as Jiakou, Civet, Castrium, Ambergris, Anise essential oil, Angri essential oil, Iran essential oil, Iris essential oil, Wikiyo essential oil, Orange essential oil, Kananga essential oil, Power Laue essential oil , Cardamom essential oil, guayata wood essential oil , Cumin essential oil, black letter essential oil, key skin essential oil, cinnamon essential oil, geranium essential oil, copaiba balsam essential oil, coriandel essential oil, perilla essential oil, cedarwood essential oil, citronella essential oil, jasmine essential oil, gingergrass essential oil, cedar essential oil, spearmint essential oil, Western Hearts Power essential oil, Otsuki perfume essential oil, tuberose essential oil, clove essential oil, orange flower essential oil, winter green essential oil, trout balsam essential oil, Baturi lily essential oil, rose essential oil, palmarosa essential oil, cocoon essential oil, hiba essential oil, sandalwood essential oil,
  • a red cabbage dye for example, as a colorant / colorant, a red cabbage dye, a red rice dye, an akane dye, an anato dye, a squid ink dye, a turquoise dye, an enji dye, a krill dye, an amber dye, a caramel, a gold, a silver, a gardenia dye, Corn pigment, onion pigment, tamarind pigment, spirulina pigment, buckwheat whole plant pigment, cherry pigment, laver pigment, hibiscus pigment, grape juice pigment, marigo mulberry pigment, purple imo pigment, purple potato pigment, lac pigment, rutin, etc. Is mentioned.
  • sweeteners include sugar, sweet tea, fructose, arabinose, galactose, xylose, mannose, maltose, honey, glucose, miraculin, monelin and the like.
  • examples of the nutrient fortifier include shell calcined calcium, cyanobacamine, yeast, wheat germ, soybean germ, egg yolk powder, hemicellulose, heme iron and the like.
  • pentadecanoic acid glyceride coleus extract, gentian extract, macca extract, roya jelly extract, kumazasa extract, t-flavanone, 6-penzinoreaminopurine, assembly extract, carpronium chloride, minoxidil, finasteride , Adenosine, nicotinamide, mulberry root extract, dio extract, and 5-aminolevulinic acid.
  • Animal and plant extracts include Asenjak (Asen Yaku), Ashitaba, Acerola, Altea, Al Niki, avocado, Amachiya (Amacha), Aloe, Aloe Vera, Nettle, Ichiyo (Ginkgo Leaves, Ginkgo), Wikiyo (Ayaka) , Turmeric (Usukin), Uspasaishin (spicy), Japanese apricot (Plum), Vulture mouth oak, mulberry, Neubara (Nonjutsu), hikikoshi (life-prolonging grass), ogi (Twilight), Koganebana (Ogon), yamazakura (cherry skin) ), Kihada (Yellow), Oulen (Huang Ren), Ginseng (Ginseng), Oto Girisou (Little Cut Grass), Odorico Sou, Dutch Garashi, Orange, Itohimehagi (Distance), Tubo Dasa (Summer Blossom), Tsur
  • an ultraviolet absorber amyl benzoate, octyl paraaminobenzoate, ethylene glycol salicylate, phenyl salicylate, octyl salicylate, benzyl salicylate, butylphenyl salicylate, homomenthyl salicylate, benzyl quine, paramethoxykelate 2 —Ethoxyethyl, normethoxykelate octyl, diparamethoxykelate mono-2-ethyl glyceryl hexanoate, isopropyl paramethoxykelate, diisopropyl'diisopropylcinnamate ester mixture, urocanic acid, urocanic acid ethyl, hydroxy Methoxybenzophenone, hydroxymethoxybenzophenonesulfonic acid and its salts, dihydroxymethoxybenzophenone, dihydroxymethoxybenzophenone sodium disulfonate, Hydr
  • Antioxidants include ascorbic acid and its salts, stearic acid ester, tocopherol and its ester derivatives, nordihydroguasertenic acid, butylhydroxytoluene (BHT), butylhydroxyanisole (BHA), para Examples include hydroxyanisole, propyl gallate, sesamol, sesamorin, gossypol.
  • Anti-inflammatory agents include glycyrrhizic acid and its salts, glycyrrhetinic acid and its salts, isopropylaminocaproic acid and its salts, allantoin, lysozyme chloride, guaizlene, methyl salicylate, ⁇ -oryzanol, and antioxidants include ⁇ Carotene, 13 carotene, y carotene, lycopene, cryptoxanthine, noretin, zeaxanthin, isazexane Carotenoids such as tin, rhodoxanthine, capsanthin, crocetin; 1,4-diazacyclooctane, 2,5-dimethylfuran, 2-methylfuran, 2,5-diphenylfuran, 1,3-dipheninoreisobenzofuran, ⁇ -tocopherol , / 3 tocopherol, ⁇ tocopherol, d to
  • the active oxygen scavenger according to the first invention of the present invention can be used as an external preparation by blending it into cosmetics, quasi drugs, medical supplies, hygiene products, and pharmaceuticals.
  • the dosage form is not limited, and ampoules , Capsules, powders, granules, pills, tablets, solids, solutions, gels, bubbles, emulsions, creams, ointments, sheets, mousses, bath preparations, and the like.
  • cosmetics, quasi-drugs, medical products, hygiene products, and pharmaceuticals include, for example, internal use external preparations, lotions, emulsions, creams, ointments, lotions, oils, packs, etc.
  • Examples include refreshing agents, gargles, liquid odors, deodorants, sanitary products, sanitary cotton, and wet tissue.
  • the cosmetic composition containing the active oxygen scavenger of the present invention can also be used as an ointment or a poultice.
  • Examples of the foods and drinks include soft drinks, carbonated drinks, nutrition drinks, fruit drinks, lactic acid drinks, ice cream, ice sherbet, power, frozen ice confectionery, soba, udon, harusame, gyoza skin , Sweet potato skins, Chinese rice cakes, instant rice cakes, rice cakes, candy, gum, chocolate, tablets, snacks, biscuits, jelly, jams, creams, baked goods, pans, etc.
  • Seafood such as clams, tuna, sardines, shrimp, bonito, sano, whales, oysters, saury, squid, akagai, scallops, arabi, yu, ichura, tokobushi, and other marine products such as power, maboko, ham, sausage ⁇ Dairy products such as processed livestock foods, processed milk, fermented milk, salad oil, tempura oil, margarine, mayonnaise, shortening, whipped cream, dressing, etc.
  • Fat and oil processed foods, seasonings such as sauces, sauces, curry, stew, oyakodon, rice cakes, miscellaneous cooking, Chinese rice bowl, bonito, tempura, eel rice, hayashi rice, oden, mabodorf, beef bowl, meat sauce, egg soup,
  • Examples include retort bouch foods such as omelet rice, dumplings, shiyumai, non-nog, meat bonore, various forms of health / nutritional supplements, health functional foods, tablets, capsules, drinks, troches and the like.
  • the active oxygen scavenger according to the first invention of the present invention is preferably applied to humans, but is applied to animals other than humans as long as each effect can be expected. That's it.
  • the active oxygen scavenger of the first invention of the present invention is not particularly limited, but nonionic surfactant, lower alcohol, polyhydric alcohol, olive oil, squalane, fatty acid It can be used by dissolving in natural fats and oils.
  • Nonionic surfactants include, for example, sorbitan fatty acid esters (for example, sorbitan monooleate, sorbitan monoisostearate, sorbitan monolaurate, sorbitan monolaurate).
  • sorbitan fatty acid esters for example, sorbitan monooleate, sorbitan monoisostearate, sorbitan monolaurate, sorbitan monolaurate.
  • hydrophilic hydrophilic nonionic surfactant examples include ⁇ -sorbitan fatty acid esters (for example, ⁇ -sorbitan monooleate, ⁇ -sorbitan monostearate, ⁇ -sorbitan monooleate, ⁇ —Sorbit fatty acid esters (eg, ⁇ —Sorbit monolaurate, ⁇ —Sorbit monooleate, ⁇ ⁇ —Sorbit pentaoleate, ⁇ —Sorbit monostearate, etc.); Glycerin fatty acid esters (eg, ⁇ -glycerol monostearate, ⁇ -glycerol monoisostearate, ⁇ -monooleate, etc.); ⁇ -fatty acid esters (eg, ⁇ -distearate) , ⁇ —Monozi ⁇ ⁇ ⁇ -alkynole ethers (e.g., ⁇ -lauryl ether, ⁇ -oleyl ether, ⁇ -stearyl
  • Tetra POP-ethylene diamine amine condensates for example, Tetronic, etc.
  • POE-castor oil-cured castor Oil derivatives eg POE—castor oil, POE—hardened castor oil, POE—hardened castor oil monoisoss) AOETO, POE—hardened castor oil triisostearate, POE—hardened castor oil monopyroglutamic acid monoisostearic acid diester, POE—hardened castor oil maleic acid, etc.
  • POE honey bees' lanolin derivatives (eg, POE—sorbite beeswax etc.) ); Al Powered olamide (eg coconut oil fatty acid diethanolamide, lauric acid monoethanolamide, fatty acid isopropanolamide, etc.); POE propylene glycol fatty acid ester; POE-alkylamine; POE fatty acid amide; sucrose fatty acid ester; alkyl ethoxydimethylamine oxide
  • Examples of the lower alcohol include ethanol, propanol, isopropanol, isobutyl alcohol, t-butyl alcohol and the like.
  • polyhydric alcohol examples include dihydric alcohols (for example, ethylene glycol, propylene glycol, trimethylene glycol, 1,2-butylene glycol, 1,3-butylene glycolanol, tetramethylene glycolanol, 2, 3 Butylene glycolanol, pentamethylene glycolanol, 2 butene 1,4 dianol, hexylene glycolanol, octylene glycol, etc .; Trivalent alcohol (eg, glycerin, trimethylolpropane, etc.); Tetravalent alcohol (eg, 1, 2, 6 hexanetriol, etc.
  • dihydric alcohols for example, ethylene glycol, propylene glycol, trimethylene glycol, 1,2-butylene glycol, 1,3-butylene glycolanol, tetramethylene glycolanol, 2, 3 Butylene glycolanol, pentamethylene glycolanol, 2 butene 1,4 dianol, hexylene glycolanol,
  • pentaerythritol, etc. pentavalent alcohol
  • pentavalent alcohol eg, xylitol, etc.
  • hexavalent alcohol eg, sonorebitanol, mannitol, etc.
  • polyhydric alcohol polymer eg, diethylene glycol, diethylene glycol, etc.
  • divalent alcohol alkyl ethers eg, ethylene glycol monomethyl ether, ethylene glycol monomethenole
  • Etherenole ethylene glycol monobutinole etherenole, ethylene glycol mononomonomono etherenole, ethylene glycol mononomonohexenoreethenore, ethylene glyconoremono 2-methylenohexen
  • Dihydric alcohol ether esters for example, ethylene glycol monomethyl ether acetate, ethylene glycol monoethyl ether acetate, ethylene glyconomonomonobutylenoate acetate, ethylene glycol monomonoinoatenoate acetate, ethylene glyconoresazia
  • Bait ethyleneglycolenoresuccinate, diethylene glycol monoethyl ether acetate, diethylene Recall monobutyl etherate acetate, propylene glycol monomethinoate etherate acetate, propylene glyconomonoethylenoate acetate, propylene glycol monopropinoate acetate, propylene glycol monophenyl ether acetate, etc.
  • glycerin mono Alkyl ethers for example, minomino ethanol, cerakil alcohol, batyl alcohol, etc.
  • sugar alcohols for example, sonorebitole, multitonole, manoletotrios
  • oils avocado oil, olive oil, sesame oil, camellia oil, evening primrose oil, turtle oil, madame demian nut oil, corn oil, mink oil, rapeseed oil, egg yolk oil, persic oil, oat germ oil , Southern power oil, castor oil, Amani oil, safflower oil, cottonseed oil, Eno oil, soybean oil, peanut oil, tea seed oil, cocoa oil, rice bran oil, kiri oil, jojoba oil, cocoa butter, coconut oil, Animal and vegetable oils such as horse oil, palm oil, palm kernel oil, beef tallow, sheep fat, pork tallow, lanolin, whale wax, beeswax, carnauba roux, moclaw, candelilla roux, squalane, and hardened oils thereof.
  • Examples of higher fatty acids include lauric acid, myristic acid, normitic acid, oleic acid, linoleic acid, linolenic acid, stearic acid, behenic acid, 12-hydroxystearic acid, isostere.
  • Examples include aspartic acid, undecinic acid, tolic acid, eicosapentaenoic acid, docosahexaenoic acid.
  • Examples of the higher alcohols include laurinorenolechol, cetyl alcohol, stearinorenole monole, beheninoleanoreconole, myristinoleanore: 3-nore, reino reinorenorenore, ceto stearinorenoreconole, Jojobano Reconole, Lanolinano Reconole, Notino Reano Renore, 2-Desino Rete Tethecinore, Cholesterol Nore, Phytosterol Nore, Isostearinore Alcohol, etc.
  • Synthetic esters include, for example, cetyl octanoate, octyldodecyl myristate, isopropyl myristate, myristyl myristate, isopropyl palmitate, butyl stearate, hexyl laurate, decyl orenate, dimethyl octanoate, cetyl lactate, Examples include myristyl lactate.
  • the silicone include chain polysiloxanes such as dimethyl polysiloxane and methinorefinole polysiloxane, cyclic polysiloxanes such as decamethylenocyclopolysiloxane, and three-dimensional network structures such as silicone resins.
  • activation is intended to maintain or enhance cell function or activity. As a result, it is possible to suppress a decrease in cell function and cell activity, that is, to suppress cell aging. Therefore, “activator” is synonymous with “cell activator” and has utility as “anti-aging agent”.
  • activation, anti-aging in skin cells refers to reducing wrinkles and sagging of the skin by reducing the deterioration of the function of skin cells associated with the accumulation of structural changes in the substrata membrane due to aging and photoaging. This refers to the prevention and improvement of curing, etc., and maintaining the skin's elasticity and youthful healthy skin.
  • the activator according to the second invention of the present invention is not particularly limited as long as it contains an extract of an Asteraceae plant as an active ingredient, and among them, an activator contains an extract of a genus Camellia plant as an active ingredient. preferable.
  • the Asteraceae plants include the genus Dendranthema, Aster, Calend dula, Callistephus, Carthamus, Cosmos, and Dahlia. ), Gazania, Gerbera, Helianth us, Senecio, Zinnia, Achillea, Agratam Genus Ageratum), Kaizoic genus (Ammobium), Daisies (Bellis), Cageno urea, Cirsium, Coreopsis, Dimorp hotheca, Echinops , Felicia, Gaillar dia, Gymnaster, Helichrysum, Rholiperum, Liatris, Rudbeckia, Strokesia (Stokesia), Tagetes genus (Cacalia), moss genus (Miricacalia), genus Syneilesis, etc. Among them, bat genus power S is desirable.
  • the plants of the genus Komorisou include bat Morisou, Momijigasa, Nikkomori, Tamabuki, Innuduna, Izkanikomori, Okanikomori, Taimingasa, Kushikomorisou, Tebacomomijigasa, Nishinoyama Taimingasa, Mikamimori Etc.
  • Momijigasa which is preferred by edible plants, is suitable.
  • Momijigasa is also known as Sidke, Sidki, Sidkina, Shindoki, Kinoshita, Toukichi, Tokichina, Sudoke, Sitgi, Sudoke, and is a asteraceae plant widely distributed in Japan and eaten as a wild vegetable. Has been.
  • the extract used in the second invention of the present invention can be obtained using the whole plant of Asteraceae as a raw material. It is also possible to separate the plant body from large rhizomes, stems, and leaves to obtain each partial force and extract, and the extract may be obtained using a combination of each part as a raw material. Since the present inventors have found that among the roots, stems, and leaves of Momijigasa, particularly the leaves have many active ingredients, it is preferable to obtain an extract from the leaves as raw materials.
  • the plant of the Asteraceae plant may be used in a raw state, a frozen state, or a lyophilized state. Preferably, it is in a raw state.
  • the plant body When preparing an extract from a raw or frozen asteraceae plant, the plant body may be first crushed. A mixer, homogenizer, mortar, etc. can be used for crushing plants. The freeze-dried product may be crushed into powder after the freeze-drying operation.
  • a solvent may be used to obtain an extract from a plant.
  • the extraction method using a solvent includes a method of extraction by impregnation in a cooled or heated state at room temperature, a method of extraction using a distillation method such as steam distillation, a compression method of obtaining an extract by pressing, a supercritical fluid or Asia Methods such as extraction using a critical fluid can be suitably used, and these methods can be used alone or in combination of two or more.
  • extraction is performed at room temperature or under heating for 1 to 1 month, preferably 1 to 5 days, using 5 to 100 ml of solvent for crushed raw material lg.
  • the solvent used for the extraction is not particularly limited as long as the active ingredient can be effectively extracted, but is water, an organic solvent miscible with water, such as a lower alcohol such as ethanol, or A mixed solution thereof, or an organic solvent immiscible with water such as ethyl acetate can be mentioned.
  • monohydric alcohols such as methanol, isopropanol, isobutanol, n-hexanol, methylamyl alcohol, 2-ethylbutanol and n-octyl alcohol; glycerin, ethylene glycol, ethylene glycol mono A polyhydric alcohol such as methinorethenole, propylene glycolenole, propylene glycolenomonomethinoleatenore, propyleneglycolenomonoethylenoreatenore, triethyleneglycolenole, 1,3-butyleneglycolinole, hexylene glycol or the like Derivatives; Ketones such as acetone, methyl ethyl ketone, methyl isobutyl ketone, and methyl-n-propyl ketone; Esters such as isopropyl acetate; Ethyl ether, isopropyl ether, n-
  • Ethers such as squalene, petrolatum, paraffin wax, paraffin oil; vegetable oils such as olive oil, wheat germ oil, rice oil, sesame oil, mackerel damian nut oil, almond oil, coconut oil; beef fat, pig Examples thereof include animal fats such as fat and whale oil.
  • a polar solvent to which inorganic salts such as phosphate buffered saline are added and a solvent to which a surfactant is added can be used, and there is no particular limitation. Considering safety for use in food and drink, application to the human body including application to the skin, etc., it is particularly preferable to extract with water and / or alcohol! /.
  • the extraction operation may be performed step by step. For example, extraction is performed with an organic solvent that is miscible with water, such as a lower alcohol such as ethanol, the mixture is concentrated with an evaporator, and an organic solvent that is immiscible with water, such as ethyl acetate, and water are added. May be separated into layers.
  • an organic solvent that is miscible with water such as a lower alcohol such as ethanol
  • the mixture is concentrated with an evaporator
  • an organic solvent that is immiscible with water such as ethyl acetate
  • the extract of the Asteraceae plant thus obtained may be used as it is, and may be subjected to a purification treatment such as deodorization and decolorization as long as the effect is not lost. Also extract In order to increase the concentration of the active ingredient therein, the obtained extract can be further subjected to means such as concentration, liquid-liquid distribution, adsorption chromatography, normal phase or reverse phase chromatography.
  • These extracts, purified products or fractions thereof may be dried to solids by removing the solvent from these extracts, and may also be in a form solubilized in a solvent such as alcohol. Can be used in the form of an emulsion.
  • Power rolls are known to be mainly contained in the Asteraceae plants, in addition to Momigasa, Cacana adenostyloides ⁇ Cacalia
  • the activator according to the present invention preferably contains a power roll as an active ingredient.
  • the strength roll concentration in the extract can be measured using HPLC as described in Example 1, for example.
  • a refined product with an increased content of a power roll as an active ingredient or a pure power carol product as an active ingredient is also suitable as an active ingredient of the activator according to the second invention of the present invention.
  • the power roll can be chemically synthesized using talesol methyl ester as a raw material (see Chem. Lett., 1317 (1975)).
  • the power roll obtained by chemical synthesis can also be suitably used as an active ingredient of the activator according to the present invention.
  • An extract of the Asteraceae plant obtained as described above, a refined product with an increased content of power rolls, or a pure product of power rolls can be used as an activator as it is. It is preferable to combine with cosmetics, quasi-drugs, pharmaceuticals, and foods and drinks in the form of a composition.
  • the concentration of the extract of the Asteraceae plant is appropriately selected depending on the degree of absorption, degree of action, product form, frequency of use, etc., and is not particularly limited. It is sufficient to select the blending concentration within a range that does not impair the cell activation effect.
  • the force roll concentration is from 0.001 to 100,000 mg / ml, preferably from 0.00 to 10 ng / ml, more preferably Is 0. l to lng / ml.
  • a third invention of the present invention is [1] a method for producing a plant extract containing a power roll, and a fourth invention of the present invention is [2] a collagen production promoter.
  • the method for producing a plant extract containing a power roll according to the third invention of the present invention comprises a step of obtaining a secondary extract from a plant extract containing a power carol using a mixture of water and a hydrophilic organic solvent.
  • a plant extract containing a power roll used in the above process is acceptable. It is only necessary that the chlorophyll content of the secondary extract obtained after the implementation of the above process is reduced as compared with the chlorophyll content contained in the extract.
  • the above “step for obtaining a secondary extract from a plant extract containing a power roll using a mixture of water and a hydrophilic organic solvent” is hereinafter referred to as “step 1”.
  • the power roll is known to be the main component of the extract of Momijigasa and has a structure represented by the formula (I).
  • Momijigasa is a plant belonging to the genus Komorisou, and is widely distributed in Japan and eaten as a wild vegetable. It is known that power rolls are also contained in asteraceae plants other than Momigasa, for example, Cacalia
  • the "plant extract containing a power roll" used in step 1 is a plant extract, As long as it contains a tool. Specifically, the primary extract from the plant containing a power roll is mentioned, for example. The primary extract from plants usually contains a large amount of chlorophyll, so it can be reduced to chlorophyll content by applying it to Step 1, and the secondary extract with high power roll content. Can be obtained.
  • the secondary extract obtained by subjecting the primary extract from the plant to Step 1 may be subjected to Step 1 again as a "plant extract containing a force carol"! /.
  • the second secondary extract thus obtained has a lower chlorophyll content than the first secondary extract.
  • the target value of the chlorophyll content may be appropriately set according to the purpose of use of the plant extract containing the power roll finally obtained. In plant extracts containing force rolls, it is preferable to reduce the chlorophyll content as much as possible. Most preferably, it is below the detection limit when measured using a known chlorophyll concentration measurement method. Therefore, for example, the target numerical value of the chlorophyll content may be set as the detection limit value.
  • the chlorophyll concentration in the plant extract can be quantified by, for example, dissolving the plant extract in a suitable solvent and measuring the absorbance.
  • a method for obtaining a primary extract from a plant containing a power roll is not particularly limited, and a known method can be appropriately selected and used.
  • plant parts as raw materials may be separated from rhizomes, stems, and leaves that are larger than the whole plant body, and the respective parts may be used as raw materials. Further, a material obtained by appropriately combining the respective parts may be used as a raw material. Since the present inventors have found that there are many active ingredients in roots, stems, and leaves, particularly leaves, when using Momijigasa as a raw material, it is preferable to obtain an extract using leaves as a raw material.
  • the raw material plant may be in a raw state, a frozen state, or a lyophilized state. Preferably, it is in a raw state.
  • the plant body When preparing a primary extract from a raw or frozen plant body, the plant body may be first crushed. A mixer, a homogenizer, a mortar, etc. can be used for crushing a plant body. If freeze-dried, freeze-dry After the operation, the powder may be crushed.
  • Examples of a method for obtaining a primary extract from a plant include a method using a solvent.
  • the extraction method using a solvent includes a method of extraction by impregnation in a cooled or heated state at room temperature, a method of extraction using a distillation method such as steam distillation, a compression method of obtaining an extract by pressing, a supercritical fluid or Extraction methods using subcritical fluids can be suitably used, and these methods can be used alone or in combination of two or more.
  • a distillation method such as steam distillation
  • a compression method of obtaining an extract by pressing a supercritical fluid or Extraction methods using subcritical fluids
  • these methods can be used alone or in combination of two or more.
  • 5 to 100 ml of solvent is used for the crushed raw material lg, and extraction is performed at room temperature or under heating for 1 hour to 1 force for 1 month to 1 month, preferably 1 to 5 days.
  • the solvent used in the extraction is not particularly limited as long as it is a solvent from which the power roll, which is an active ingredient, can be effectively extracted, but water or water such as ethanol or a lower alcohol. Examples thereof include organic solvents that are miscible, or mixtures thereof, or organic solvents that are immiscible with water, such as ethyl acetate.
  • monohydric alcohols such as methanol, isopropanol, isobutanol monole, n-hexanol, methenoleaminoleanoreconole, 2-ethylbutanol, n-octyl alcohol; glycerin, ethylene glycol , Ethylene glycol monomethino ethenore, propylene glycol nore, propylene glycol eno mono ethino enore, propylene glycol eno mono chineno ethenore, triethylene glycol nore, 1,3 butylene glycol mononore, hexylene glycol, etc.
  • monohydric alcohols such as methanol, isopropanol, isobutanol monole, n-hexanol, methenoleaminoleanoreconole, 2-ethylbutanol, n-octyl alcohol
  • Ketones such as acetone, methyl ethyl ketone, methyl isobutyl ketone, methyl n propyl ketone; Esters such as isopropyl acetate; Ethyl ether, Isopropyl ether, n Butyl ether Ethers such as oil; hydrocarbons such as squalene, petrolatum, paraffin wax, paraffin oil; vegetable oils such as olive oil, wheat germ oil, rice oil, sesame oil, mackerel damian nut oil, almond oil, coconut oil; Examples include animal fats such as beef tallow, pork tallow and whale oil.
  • a polar solvent to which inorganic salts such as phosphate buffered saline are added and a solvent to which a surfactant is added can be used without any particular limitation. Considering safety for use in food and drink, application to the human body including application to the skin, etc., it is particularly preferable to extract with water and / or alcohol! /.
  • the extraction operation may be performed step by step! /.
  • extraction is performed with an organic solvent that is miscible with water, such as a lower alcohol such as ethanol, and the mixture is concentrated with an evaporator or the like.
  • An organic solvent immiscible with water, such as oxyethyl, and water may be added and separated into an organic solvent layer and an aqueous layer.
  • the primary extract thus obtained is preferably concentrated to dryness by removing the solvent.
  • step 1 Next, step 1 will be described.
  • Step 1 for example, a secondary extract is obtained using a mixture of water and a hydrophilic organic solvent with respect to a primary extract from a plant containing a power roll obtained as described above.
  • the plant extract containing the power roll used in step 1 is not limited to the primary extract from the plant, but also includes the secondary extract that has already been subjected to the extraction process in step 1. Is preferred.
  • hydrophilic organic solvent such as methanol, ethanol, propyl alcohol, I isopropyl alcohol, lower alcohols having 1 to 5 carbon atoms in butanol; acetone, lower aliphatic ketones such as methylcarbamoyl Ruechiruketon; 1, 3
  • examples include polyhydric alcohols having 2 to 5 carbon atoms such as butylene glycol, propylene glycol, and glycerin. These may be used alone or in combination of two or more. Good.
  • the hydrophilic organic solvent used for the secondary extraction is particularly considered ethanol, acetone, isopropyl alcohol, propylene glycol, It is preferable to use butylene glycol and butanol.
  • Moisture content, ie chlorophyll content in the plant extract containing the power roll that the chlorophyll content in the secondary extract was subjected to step 1 The water content of the mixture of water and hydrophilic organic solvent, which will be further reduced, varies depending on the type of hydrophilic organic solvent, the type of raw material plant, the part of the raw material plant used, etc. It is preferable to confirm it.
  • hydrous ethanol having an ethanol content of 45% or more, more preferably hydrous ethanol having an ethanol content of 50% to 70%, more preferably Hydrous ethanol with an ethanol content of 55% to 65%.
  • the first step is, for example, adding water and It can be carried out by adding a mixture of hydrophilic organic solvents and allowing to stand, and then collecting the supernatant.
  • Step 1 By performing Step 1, it is possible to obtain a plant extract having a high content of power rolls and a low content of chlorophyll at a low cost in terms of efficiency. Further, by repeating Step 1, a plant extract containing a power roll containing almost no chlorophyll can be obtained.
  • the chlorophyll concentration in the plant extract can be quantified by, for example, dissolving the plant extract in a suitable solvent and measuring the absorbance.
  • the obtained extract is further concentrated and liquid-liquid distributed as desired. It can also be subjected to means such as adsorption chromatography, normal phase or reverse phase chromatography.
  • a method for producing a plant extract containing a power roll according to the third invention of the present invention comprises a step 1 (secondary extraction from a plant extract containing a power roll using a mixture of water and a hydrophilic organic solvent.
  • a step 1 secondary extraction from a plant extract containing a power roll using a mixture of water and a hydrophilic organic solvent.
  • contaminants that are more hydrophilic than the power roll are removed from the plant extract containing the power roll using water or a mixture of water and a hydrophilic organic solvent. It is preferable to include a step of leaving. By adding this step, it is possible to obtain a plant extract containing a power roll in which both the chlorophyll content and the highly hydrophilic contaminant content are low.
  • Step 2 the above-mentioned “step of removing contaminants having a higher hydrophilicity than the power color from water or a mixture of water and a hydrophilic organic solvent from the plant extract containing the power roll”
  • Step 2 the above-mentioned “step of removing contaminants having a higher hydrophilicity than the power color from water or a mixture of water and a hydrophilic organic solvent from the plant extract containing the power roll”.
  • the “plant extract containing a power roll” used in Step 2 is a temporary extract obtained from a plant containing a power carol described above, or a secondary extraction that has already been subjected to the extraction process of Step 1. And an extract obtained by repeatedly performing the extraction process in Step 1.
  • the “plant extract containing a power roll” to be used in step 2 is a concentrated dry product from which the solvent has been removed.
  • Step 2 may be provided before Step 1 or after Step 1.
  • step 2 may be provided between the previous step 1 and the subsequent step 1.
  • the hydrophilic organic solvent suitably used in Step 2 is the same as that exemplified in the description of Step 1.
  • the preferred water content in the mixture of water and hydrophilic organic solvent that is, the water content of the mixture of water and hydrophilic organic solvent that can efficiently remove impurities that are more hydrophilic than the power roll is Therefore, it is preferable to preliminarily check the optimal water content each time.
  • hydrophilic solvent when ethanol is used as the hydrophilic solvent, it is preferable to use hydrous ethanol having an ethanol content of 40% or less, more preferably hydrous ethanol having an ethanol content of 10% to 30%, and even more preferably. It is hydrous ethanol with an ethanol content of 15% to 25%.
  • water containing no hydrophilic organic solvent can be used as the extraction solvent.
  • the second step for example, water or a mixture of water and a hydrophilic organic solvent is added to a plant extract containing a power roll used in this step, and the mixture is allowed to stand, and then the precipitate is recovered. Can be implemented. The removal of highly hydrophilic impurities from the power roll can be confirmed by the decrease in the weight of the extract.
  • the plant extract containing the power roll obtained through the first step or the plant extract containing the power roll obtained through the first step and the second step removes the solvent.
  • it can be made into a dried product, and further, it can be used in a form solubilized in a solvent such as alcohol or an emulsion.
  • the method for producing a plant extract containing a power roll according to the third invention of the present invention is applicable to a power plant that can be applied to any plant as long as it contains a power carol. Is preferred.
  • Asteraceae plants there are Arctoseca, Akinokirinso, Akinonogeshi, Azami,
  • Chrysanthemum genus Hydrobana genus, Greater thistle genus, Felicia genus, Fuchsia poppo genus, Fuchsia genus, Fuchus genus, Futana genus, Futama dandelion genus, Francis genus, Buddlesia genus, Ragweed genus, Helenium genus, Safflower genus Porimunia genus, Mamekamitsure
  • Misumigusa Mizuhimabori, Miyamamena, Muchasomoghi, Mugiwaragiku, Metakarakoma, Menamomi, Melanpodium, Mokuyungiku, Mokubiyakkou, Momijihaguma, Yakuno-gama, Amanagambipha Genus, wildflower genus, yuryopus genus, genus genus, mugwort genus, mugwort genus, dandelion genus, but it is not particularly limited to the family Asteraceae as long as it contains a power roll. Is preferred.
  • bats are bats, maples, powers, tamabuki, inuduna, izkanikomori, okanakumori, taimingasa, kushikoumoriso, tebacomomijigasa, nishinoyamataiminga, It is done.
  • Momijigasa which is preferred by edible plants, is suitable. The best mode for carrying out the fourth invention of the present invention will be described.
  • the collagen production promoter according to the fourth invention of the present invention only needs to contain a force roll as an active ingredient.
  • the power roll can be obtained as a plant extract containing the power roll by the production method according to the third aspect of the present invention.
  • a refined product with an increased content of a power roll as an active ingredient, or a pure power carol product as an active ingredient is also used as an active ingredient of the collagen production promoter according to the fourth invention of the present invention. It can be used suitably.
  • the power roll can be chemically synthesized using talesol methyl ester as a raw material.
  • a force roll obtained by chemical synthesis can also be suitably used as an active ingredient of the collagen production promoter according to the fourth invention of the present invention.
  • the plant extract containing a powerful roll by the production method according to the third aspect of the present invention described above can be used as a collagen production promoter as it is.
  • the concentration of the plant extract containing force carol is appropriately selected depending on the degree of absorption, degree of action, product form, frequency of use, etc., and is not particularly limited. It is sufficient to select the blending concentration within the range that does not impair the collagen production promoting action.
  • the force roll concentration is 0.001 to 100,000 ng / ml, preferably 0.01 to 10 ng / ml, more preferably 0. 01—Use 1 ng / ml.
  • the activator according to the second invention of the present invention and the collagen production promoter according to the fourth invention of the present invention are formulated into cosmetics, quasi-drugs, pharmaceuticals, and foods and drinks. It is preferable to carry it out in the form. The following forms will be described.
  • the dosage form is not limited, and ampules, capsules, powders, granules, pills, tablets, solids, liquids, gels, bubbles, emulsions, creams, ointments, sheets, mousses, bath preparations, etc. thing Can do.
  • cosmetics, quasi-drugs, and pharmaceuticals include, for example, internal and external pharmaceutical preparations, skin lotions, emulsions, creams, ointments, lotions, oils, packs, and other basic cosmetics, face wash, Skin cleansers, shampoos, rinses, hair treatments, hair creams, pomades, hair sprays, hair styling agents, permanents, hair nicks, hair dyes, hair restorations such as hair conditioners, cosmetics, foundations, white powder, funny hair, lipstick, Makeup cosmetics such as scarlet, eye shadow, eyeliner, mascara, eyebrows, eyelashes, cosmetics for finishing such as nail polish, perfumes, bath preparations, other toothpastes, mouth fresheners and mouthwashes Liquid odor / deodorant, hygiene products, sanitary cotton, wet tissue, etc.
  • examples of food and drink include soft drinks, carbonated drinks, nutrition drinks, fruit drinks, lactic acid drinks, ice cream, ice sherbet, power, frozen ice confectionery such as soba, udon , Harusame, Gyoza skin, Pomegranate skin, Chinese rice cake, instant rice cake, etc., rice cake, candy, gum, chocolate, tablet confectionery, snack confectionery, biscuit, jelly, jam, cream, baked confectionery, bread, etc.
  • the activator according to the second invention of the present invention or the collagen production promoter according to the fourth invention of the present invention can be suitably applied to humans. As long as it is applicable to animals other than humans.
  • the activator according to the second invention of the present invention or the collagen production promoter according to the fourth invention of the present invention is added to the asteraceae plant extract, which is an active ingredient, as required.
  • the effects of the second or fourth invention are not impaired, cosmetics, quasi drugs and pharmaceuticals
  • the ingredients and additives used in food and drink can be used in combination.
  • bases and additives usually used in cosmetics as shown below, such as fats and oils, waxes, mineral oils, fatty acids, alcohols, esters, metal soap, gum Quality and water-soluble polymer compounds, surfactants, vitamins, amino acids, whitening agents, moisturizers, microbial culture metabolites, hydroxy acids, inorganic pigments, UV absorbers, astringents, antibacterial agents, bactericides, disinfectants, It can be used in combination with hair preparations, fragrances, pigments / colorants, sweeteners, nutrition enhancers, hair restorers, animal and plant extracts, antioxidants, anti-inflammatory agents, and the like.
  • apogad oil apogad oil, almond oil, wikiyo oil, sesame oil, olive oil, orange oil, orange rafa oil, sesame oil, kaka-age ⁇ , power mitsule oil, power lot oil, cucumber Oil, beef tallow, beef tallow fatty acid, cucumber nut oil, safflower oil, soybean oil, camellia oil, corn oil, rapeseed oil, persic oil, castor oil, cottonseed oil, peanut oil, turtle oil, mink oil, egg yolk oil, cocoa butter, palm Oil, palm kernel oil, owl, coconut oil, beef tallow, lard, hydrogenated oil, hydrogenated castor oil.
  • waxes include beeswax, carnauba wax, whale wax, lanolin, liquid lanolin, reduced lanolin, hard lanolin, candelillaro, montan wax, shellac wax and the like.
  • mineral oils examples include liquid paraffin, petrolatum, paraffin, ozokelide, ceresin, microcrystalline wax, polyethylene powder, squalene, squalane, and pristane.
  • fatty acids natural fatty acids such as lauric acid, myristic acid, normitic acid, stearic acid, behenic acid, oleic acid, 12-hydroxystearic acid, undecylenic acid, tall oil, lanolin fatty acid, and isononanoic acid
  • synthetic fatty acids such as caproic acid, 2-ethylbutanoic acid, isopentanoic acid, 2-methylpentanoic acid, 2-ethylhexanoic acid and isopentanoic acid.
  • the alcohols include natural alcohols such as ethanol, isopropanol, lauryl alcohol, cetanol, stearinoreanoleconore, oleinoleanoreconole, lanolinanoreconole, cholesterol, phytosterol, etc.
  • Synthetic alcohols such as xyldecanol, isostearyl alcohol, 2-octyldodecanol, ethylene oxide, ethylene glycol, diethylene glycol, triethylene glycol, ethylene glycol Noremonochinenoatenore, ethyleneglycolenobutinorenoatenore, diethyleneglycol noremonomethinoreatenore, diethyleneglycolenolemonotinoreatenore, polyethyleneglycolinole, propyleneoxide, propyleneglycolole, polypropyleneglycol 1, 3-butyleneglycolone, glycerin, notinorenoreconole, pentaerythritole, sonorebitonore, mannitol, glucose, sucrose and other polyhydric alcohols.
  • esters isopropyl myristate, isopropyl palmitate, butyl stearate, hexyl laurate, myristyl myristate, oleyl oleate, decyl oleate, octyldodecyl myristate, hexyl decyl octanoate Nole, cetyl lactate, myristyl lactate, jetyl phthalate, dibutyl phthalate, lanolin acetate, ethylene glycol monostearate, propylene glycol monostearate, propylene glycol dioleate and the like.
  • Examples of the metal soap include aluminum stearate, magnesium stearate, zinc stearate, calcium stearate, zinc palmitate, magnesium myristate, zinc laurate, zinc undecylenate and the like.
  • gums and water-soluble polymer compounds include gum arabic, benzoin rubber, dammar gum, guayata fat, Irish moss, kala gum, tragacanth gum, kilob gum, quinseed, agar, casein, dextrin, gelatin, pectin, Starch, carrageen, chitosan salt, sulfated chitin, phosphorylated chitin, alginic acid and its salt, hyaluronic acid and its salt, chondroitin sulfate, heparin, ethyl cellulose, methylcellulose
  • Rosenorelose crystalline senorelose, polyvinylenorenoleconole, polyvinino methinoreatenore, polybulol pyrrolidone, polybull metaaterylate, polyacrylic acid salt, polyalkylene oxide such as polyethylene oxide and polypropylene oxide or cross-linking thereof Polymers, carboxybule polymers, polyethyleneimine and the like can be mentioned.
  • an anionic surfactant (carboxylate, sulfonate, sulfate ester salt, phosphate ester salt), a cationic surfactant (ammine salt, quaternary ammonia) Um salt), amphoteric surfactant (carboxylic acid type amphoteric surfactant, sulfate ester type amphoteric surfactant, sulfonic acid type amphoteric surfactant, phosphate ester type amphoteric surfactant), nonionic surfactant Agents (ether type nonionic surfactant, ether ester type nonionic surfactant, ester type nonionic surfactant, block polymer type nonionic surfactant, nitrogen-containing type nonionic surfactant), other interfaces Activating agents (natural surfactants, derivatives of protein hydrolysates, polymeric surfactants, surfactants containing titanium'-carbon, fluorocarbon surfactants, etc.).
  • vitamin A1 retinol
  • vitamin A2 dehydroretinal
  • vitamin A2 carotene
  • lycopene provitamin A
  • vitamin B group thiamine hydrochloride
  • thiamine sulfate Vitamin B1
  • riboflavin riboflavin
  • Vitamin B2 Vitamin B2
  • pyridoxine vitamin B6
  • cyanocobalamin vitamin B12
  • folic acid nicotinic acid
  • pantothenic acid biotin
  • biotin choline
  • inositol vitamin C group
  • vitamin D group In ergocalciferol (vitamin D2), cholecalciferol (vitamin D3), dihydrotachysterol, vitamin E group, tocopherol and its derivatives, ubiquinones, vitamin K group, futonadione (vitamin K1) Menaquinone (vitamin K2), menadione (vitamin K3), menadiol (vitamin K4), and the like.
  • amino acids include norine, leucine, isoleucine, threonine, methionine, phenylalanine, tryptophan, lysine, glycine, alanine, asparagine, glutamine, serine, cysteine, cystine, tyrosine, proline, hydroxyproline, and aspartic acid.
  • amino acid derivatives such as sulphates, phosphates, nitrates, citrates, pyrrolidone carboxylic acids, etc., and gnoretamic acid, hydroxylysine, anoleginine, norenitine and histidine.
  • whitening agents include ascorbic acid or a derivative thereof, io, placental hydrolyzate, ellagic acid or a derivative thereof, kojic acid or a derivative thereof, darcosamine or a derivative thereof, arbutin or a derivative thereof, hydroxykehiic acid or a derivative thereof Derivatives such as glutathione, A / Leni force extract, gonon extract, sohakuhi extract, psycho extract, boufeque kiss, mynentake mycelium culture or extract thereof, linden extract, peach leaf extract, Examples include AGE extract, KUJIN extract, juju extract, toki extract, rye quinin extract, brilliant leaf kiss, dai sai extract, bot pi extract, cucumber bean extract, marronnier extract, virgin togiri extract, and oil-soluble licorice extract.
  • hyaluronic acid polyglutamic acid, serine, glycine, threonine, alanine, collagen, hydrokeratin collagen, hydronectin, fibronectin, keratin, elastin, royal jelly, chondroitin sulfate heparin, Glyce mouth phospholipid, Glyce mouth phospholipid, Sphingo phospholipid, Sphingo glycolipid, Linoleic acid or its esters, Eicosapentaenoic acid or its esters, Pectin, Bifidobacterium fermented product, Lactic acid fermented product, Yeast extract, Ganoderma Mycelium culture or extract thereof, wheat germ oil, avogad oil, rice germ oil, jojoba oil, soybean phospholipid, ⁇ — oryzanol, belode oyster kiss, yoku yun extract, di sai extract, taiso extract, force is
  • extracts or extracts of animals, plants, or herbal medicines include Azenak (Asenyaku), Ashitaba, Acerola, Anolethea, Arni, Avocado, Amatya (Amacha), Aloe, Aloe Vera, Nettle, Ichiyo ( Ginkgo biloba, Ginkgo), Wikiyo (Yuka), Ukon (Usukin), Uspasai Shin (Spicy), Ume (Plum), Vulture, Ganoderma, Neubara (Yutoshi), Hikikoshi (Extended Life Grass), Ogi (Yellow) , Koganebana (Ogon), Chestnut cherry (cherry bark), Kihada (Yellow), Oren (Yellow), Ginseng (Ginseng), Otogirisou (Little Cut Grass), Odoricosou, Dutch Garlic, Orange, Itohimehagi (Distance), Ubbodasa Summer hay), Tsuru
  • microbial culture metabolites include yeast extract, zinc-containing yeast extract, germanium-containing yeast extract, selenium-containing yeast extract, magnesium-containing yeast extract, rice fermentation extract, Euglena extract, and lactic acid fermented milk powder.
  • examples of ⁇ -hydroxy acids include glyconolic acid, citrate, malic acid, tartaric acid, and lactic acid.
  • anhydrous caustic acid magnesium silicate, talc, kaolin, bentonite, my strength, mica titanium, bismuth oxychloride, zirconium oxide, magnesium oxide, zinc oxide, titanium oxide, calcium carbonate
  • magnesium carbonate yellow iron oxide, red iron oxide, black iron oxide, gundiio, chromium oxide, chromium hydroxide, carbon black, calamine and the like.
  • an ultraviolet absorber ⁇ -aminobenzoic acid derivative, salicylic acid derivative, anthranilic acid derivative, coumarin derivative, amino acid compound, benzotriazole derivative, tetrazole derivative, imidazoline derivative, pyrimidine derivative, dioxane derivative
  • examples include camphor derivatives, furan derivatives, pyrone derivatives, nucleic acid derivatives, allantoin derivatives, nicotinic acid derivatives, vitamin 6 derivatives, oxybenzone, benzophenone, guaiazurene, shikonin, baicalin, noikalein, and berberine.
  • astringents include lactic acid, tartaric acid, succinic acid, succinic acid, allantoin, lead chloride, zinc sulfate, zinc oxide, calamine, ⁇ -phenol sulfonate zinc, potassium aluminum sulfate, resorcin chloride, secondary chloride Examples thereof include iron and tannic acid.
  • antioxidants include ascorbic acid and its salts, stearic acid ester, tocopherol and its ester derivatives, nordihydroguasertenic acid, butylhydroxytoluene ( ⁇ ), butylhydroxydisole ( ⁇ ) , Parahydroxyanisole, propyl gallate, sesamol, sesamorin, gossypol and the like.
  • anti-inflammatory agents include ictamol, indomethacin, kaolin, salicylic acid, sodium salicylate, methyl salicylate, acetylsalicylic acid, diphenhydramine hydrochloride, d or dl-forcenfunole, hydrocortisone, guaiazulene, camazulene, maleic acid chloro
  • anti-inflammatory agents include ictamol, indomethacin, kaolin, salicylic acid, sodium salicylate, methyl salicylate, acetylsalicylic acid, diphenhydramine hydrochloride, d or dl-forcenfunole, hydrocortisone, guaiazulene, camazulene, maleic acid chloro
  • lucerilamine glycyrrhizic acid and its salt
  • glycyrrhetinic acid and its salt and the like.
  • disinfectants include atalinol, io, benzalkonium chloride, benzethonium chloride, methyl rosaniline chloride, cresol monole, calcium dalconate, chlorhexidine dalconate, sulfamine, mercurochrome, ratatoferrin or its addition. moisture Examples include scraps.
  • a hair preparation selenium disulfide, alkylisoquinolinium bromide, zinc pilichin, bifuenamine, thianthol, castari tincture, pepper tincture, pepper tincture, quinine hydrochloride, strong ammonia water
  • examples include potassium bromate, sodium bromate, and thiodaricholic acid.
  • perfumes natural animal perfumes such as Jiakou, Civet, Castrium, Ambergris, Anise Essential Oil, Angri Essential Oil, Iran Essential Oil, Iris Essential Oil, Wikiyo Essential Oil, Orange Essential Oil, Kananga Essential Oil, Power Laue Essential Oil , Cardamom essential oil, guayata wood essential oil, cumin essential oil, black letter essential oil, key skin essential oil, cinnamon essential oil, geranium essential oil, copaiba balsam essential oil, coriandel essential oil, perilla essential oil, cedarwood essential oil, citronella essential oil, jasmine essential oil, gingergrass essential oil, cedar essential oil , Spearmint essential oil, Western heart force essential oil, Otsuka perfume essential oil, tuberose essential oil, clove essential oil, orange flower essential oil, winter green essential oil, trout balsam essential oil, batile lily essential oil, rose essential oil, palmarosa essential oil, persimmon essential oil, hiba essential oil, Sandalwood essential oil, Petite
  • red cabbage dye red rice dye, akane dye, anato dye, squid ink dye, turmeric dye, chenju dye, krill dye, amber dye, caramel, gold, silver, gardenia dye, Corn pigment, onion pigment, tamarind pigment, spirulina pigment, buckwheat whole plant pigment, cherry pigment, laver pigment, hibiscus pigment, grape juice pigment, marigo mulberry pigment, purple imo pigment, purple potato pigment, lac pigment, rutin, etc. Is mentioned.
  • sweetener examples include sugar, sweet tea, fructose, arabinose, galactose, xylose, mannose, maltose, honey, glucose, miraculin, monelin and the like.
  • examples of the nutrient fortifier include shell calcined calcium, cyanobactamine, yeast, wheat germ, soybean germ, egg yolk powder, hemicellulose, heme iron and the like.
  • the activator according to the second invention of the present invention or the collagen production promoter according to the fourth invention of the present invention is one that is suitably applied to humans. As far as possible, it can also be applied to non-human animals.
  • the activator according to the second invention of the present invention or the collagen production promoter according to the fourth invention of the present invention is not particularly limited, but a nonionic surfactant or lower alcohol is not particularly limited. It can be used by dissolving in alcohol, polyhydric alcohol, or natural oils such as olive oil, squalane and fatty acids.
  • nonionic surfactant examples include sorbitan fatty acid esters (for example, sorbitan monooleate, sorbitan monoisostearate, sorbitan monolaurate, sorbitan monopalmitate, sorbitan monostearate, sorbitan).
  • sorbitan fatty acid esters for example, sorbitan monooleate, sorbitan monoisostearate, sorbitan monolaurate, sorbitan monopalmitate, sorbitan monostearate, sorbitan).
  • glycerin polyglycerin fatty acids eg mono cottonseed oil fatty acid glycerin, Glyceryl monoellate, glyceryl sesquioleate
  • hydrophilic hydrophilic nonionic surfactant examples include ⁇ -sorbitan fatty acid esters (for example, ⁇ -sorbitan monooleate, ⁇ -sorbitan monostearate, ⁇ -sorbitan monoo).
  • ⁇ sorbitan tetraoleate, etc .
  • ⁇ sorbite fatty acid esters eg, ⁇ —sorbite monolaurate, ⁇ —sorbite monooleate, ⁇ —sorbitol pentaoleate, ⁇ —sorbite monostearate, etc.
  • ⁇ -glycerin fatty acid esters eg, ⁇ -glycerol monostearate, ⁇ -glycerol monoisostearate, ⁇ -glycerol triisostearate, etc.
  • -fatty acid esters eg, ⁇ —Disteare , POE monodiolate, ethylene glycol distearate, etc .
  • POE alkynole ethers eg, POE lauryl ether, POE oleyl ether, POE-stearyl ether, POE behenyl ether, POE-2-Otachidodecyl ether, POE cholest
  • Tetra POP ethylene diamine condensates (eg, Tetronic etc.); POE castor oil hardened castor oil derivatives (eg, POE castor) Oil, POE hydrogenated castor oil, POE hydrogenated castor oil monoisostearate, POE Castor oil triisostearate, POE hydrogenated castor oil monopyroglutamic acid monoisostearic acid diester, POE hydrogenated castor oil maleic acid, etc.); POE beeswax 'lanolin derivatives (eg POE sorbite beeswax etc.); Coconut oil fatty acid diethanolamide, lauric acid monoethanolamide, fatty acid isopropanolamide, etc.); POE propylene glycol fatty acid ester; POE-alkylamine; POE fatty acid amide; sucrose fatty acid ester; alkylethoxydimethylamine oxide; trioleyl phosphoric acid, etc. Can be mentioned.
  • POE castor oil hardened castor oil derivatives
  • Examples of the lower alcohol include ethanol, propanol, isopropanol, isobutyl alcohol, t-butyl alcohol, and the like.
  • polyhydric alcohol examples include divalent alcohols (for example, ethylene glycol, propylene glycol, trimethylene glycol, 1,2-butylene glycol, 1,3-butylene glycolol, tetramethylene glycolol, 2, 3 Butylene glycolanol, pentamethylene glycolanol, 2 butene 1,4 dianol, hexylene glycolanol, octylene glycol, etc .; trivalent alcohol (eg, glycerin, trimethylolpropane, etc.); tetravalent alcohol (eg, 1, 2, 6 hexanetriol, etc.
  • divalent alcohols for example, ethylene glycol, propylene glycol, trimethylene glycol, 1,2-butylene glycol, 1,3-butylene glycolol, tetramethylene glycolol, 2, 3 Butylene glycolanol, pentamethylene glycolanol, 2 butene 1,4 dianol, hexylene glycolanol,
  • pentaerythritol, etc. pentavalent alcohol
  • pentavalent alcohol eg, xylitol, etc.
  • hexavalent alcohol eg, sonorebitanol, mannitol, etc.
  • polyhydric alcohol polymer eg, diethylene glycol, diethylene glycol, etc.
  • divalent Alcohol alkyl ethers for example, ethylene glycol monomethyl ether, ethylene glycol monoethyl enoate ethere, ethylene glyco mono mono butyl mono ether, ethylene glycol mono mono mono eno mono ether, Tenoré, Ethyleneglycolenomono 2-Methylenohexenoleethenore, Ethylene
  • oils avocado oil, olive oil, sesame oil, camellia oil, evening primrose oil, turtle oil, madame demian nut oil, corn oil, mink oil, rapeseed oil, egg yolk oil, persic oil, oat germ oil , Southern power oil, castor oil, Amani oil, safflower oil, cottonseed oil, Eno oil, soybean oil, peanut oil, tea seed oil, cocoa oil, rice bran oil, kiri oil, jojoba oil, cocoa butter, coconut oil, Animal and vegetable oils such as horse oil, palm oil, palm kernel oil, beef tallow, sheep fat, pork tallow, lanolin, whale wax, beeswax, carnauba roux, moclaw, candelilla roux, squalane, and hardened oils thereof.
  • Examples of higher fatty acids include lauric acid, myristic acid, normitic acid, oleic acid, linoleic acid, linolenic acid, stearic acid, behenic acid, 12-hydroxystearic acid, isostearic acid, undecinic acid, and toric acid. Eicosapentaenoic acid and docosahexaenoic acid.
  • Examples of the higher alcohol include laurino enorecol, cetyl alcohol, stearino enore monole, behenino enore enore, myristino enore: 3-nore, rei leino enore enore, ceto stearino enorenore, Jojobano Reconole, Lanolinano Reconole, Notino Reano Renore, 2-Desino Rete Tethecinore, Cholesterol Nore, Phytosterol Nore, Isostearinore Alcohol, etc.
  • Synthetic esters include, for example, cetyl octanoate, octyldodecyl myristate, isopropyl myristate, myristyl myristate, isopropyl palmitate, butyl stearate, hexyl laurate, decyl orenate, dimethyl octanoate, cetyl lactate, Examples include myristyl lactate.
  • the silicone include chain polysiloxanes such as dimethyl polysiloxane and methinorefinole polysiloxane, cyclic polysiloxanes such as decamethylenocyclopolysiloxane, and three-dimensional network structures such as silicone resins.
  • SOD Superoxide dismutase analysis kit WST (manufactured by Dojindo Molecular Technologies) was used to measure the superoxide elimination ability of the power roll. A specific method is described below. For the solutions not specifically described in the following method, those provided in the above measurement kit or those prepared according to the method of using the kit were used.
  • the power roll showed a superoxide elimination rate of 28% at a final concentration of ImM.
  • astaxanthin and vitamin E used as comparative products were precipitated in the reaction solution at the same concentration, and thus could not be evaluated. Superoxide scavenging action was not observed even at concentrations below that level.
  • the reagent solution mixed with 100 1 was preincubated at 37 ° C.
  • ImM hydrogen peroxide 100 1 and power roll solution 100 1 of each concentration were mixed, incubated at 37 ° C for 20 minutes, immediately mixed with the above reagent solution, and reacted at 37 ° C for 5 minutes.
  • Absorbance at 555 nm was measured with a microplate reader. As a control, the following conditions were also measured in the same manner.
  • the hydrogen peroxide scavenging rate of the sample was obtained by calculating the formula 100_ (S—B2) / B1 ⁇ 100.
  • S is A with hydrogen peroxide added 'sample added
  • Bl (Blank 1) is hydrogenated with hydrogen peroxide' A with MilliQ water added
  • B2 (Blank 2) is not added with hydrogen peroxide (Instead, MilliQ water added) 'Shows A for sample addition.
  • Astaxanthin was used as a substance to be compared. The results are shown in Figure 1.
  • the power roll showed a high hydrogen peroxide scavenging rate compared to wastaxanthin.
  • Vitamin E used as a comparative product was precipitated in the reaction solution at the same concentration, so it could not be evaluated, and no superoxide scavenging action was observed even at concentrations below that.
  • the singlet oxygen scavenging rate of the sample was obtained by calculating the formula 100_ (S—B2) / B1 ⁇ 100.
  • S is A with light irradiation and sample addition
  • Bl is A with light irradiation 'MilliQ water addition
  • B2 is A with light non-irradiation sample addition. Show.
  • Astaxanthin and vitamin E were used as comparative substances. The result is shown in figure 2. It was shown that the power roll has a higher singlet oxygen scavenging ability than vitamin E, although the scavenging rate is lower than that of wastaxanthin, which is known as a singlet oxygen scavenger.
  • Example 1 The strength roll was dissolved in butylene glycol at a concentration of 0.5% and allowed to stand at room temperature 'light place, room temperature' place, 4 ° C. Analysis was performed under the HPLC conditions described. The results are shown in Figure 3. It was found that the power roll showed high stability under any condition, and the active oxygen scavenger of the present invention maintained its effect for a long time.
  • a cosmetic liquid having the following composition was produced by a conventional method.
  • a serum containing no force roll was also produced by a conventional method.
  • Power roll (power roll concentration 0.01%) 1.0
  • Total amount of purified water is 100
  • An emulsion having the composition shown below was produced by a conventional method. Power roll as a control No emulsions were prepared by conventional methods.
  • Power roll (power roll concentration 0.01%) 1.0
  • Total amount of purified water is 100
  • a cream having the following composition was produced by a conventional method.
  • a cream containing no force roll was also produced by a conventional method.
  • Power roll (power roll concentration 0.01%) 1.0
  • Example 10 Preparation of maple extract
  • 250 g of Momijigasa was lyophilized and extracted with 500 ml of a mixture of methylene chloride and methanol 1: 1.
  • the concentration of the force roll was measured by HPLC analysis under the following conditions.
  • Normal human skin fibroblasts were cultured by a conventional method using a normal human skin fibroblast total kit (CA106K05, manufacturer: Cell Applications Inc. USA, importer / distributor: Toyobo Co., Ltd.).
  • an ethanol group (final concentration 0.5%) was provided.
  • an SDS-added group (final concentration: 0.1%) was provided to confirm that cell growth was inhibited by cytotoxic substances.
  • the medium was replaced with a medium containing 100 ⁇ g / mL of 3- (4,5-dimethyl-2-thiazolyl) -2,5-diphenyltetrazolium bromide (MTT) and cultured for 3 hours.
  • Formazan produced by ring-opening was extracted with 2-propanol and the absorbance at 550 nm was measured with a microplate reader. At the same time, the absorbance at 650 nm was measured as turbidity, and the cell activation effect was evaluated by the difference between the two measured values.
  • the maple extract has an excellent cell activation effect, and by applying the maple extract to the skin, it exhibits an extremely excellent anti-aging effect, It was suggested that skin wrinkles and sagging caused by aging, UV exposure, etc. can be effectively improved.
  • a cosmetic liquid having the following composition was produced by a conventional method.
  • a serum containing no Momijigasa extract was also produced by a conventional method.
  • Total amount of purified water is 100
  • An emulsion having the composition shown below was produced by a conventional method.
  • an emulsion containing no Momijigasa extract was also produced by a conventional method.
  • Total amount of purified water is 100
  • a cream having the following composition was produced by a conventional method.
  • a cream containing no Momijigasa extract was also produced by a conventional method.
  • Acid polyester 38.0 sodium edetate 0.03
  • Total amount of purified water is 100
  • a use test was conducted using the cream produced in Example 14 above.
  • the evaluation items were self-evaluation of the improvement effect on spots and kusumi.
  • a cream containing no Momijigasa extract was used blind.
  • five panelists aged 25 to 50 years used it for 1 month, and evaluated before and after use in three stages: improved, slightly improved, and no change.
  • the examples of the present invention were more effective in improving wrinkles and rough skin than the conventional comparative examples.
  • Example 15 Preparation of tartorol extract using ethanol with different water contents
  • 2 kg of Momijigasa leaves were lyophilized and stirred with a mixer to obtain 212 g of powder.
  • 4 L of ethanol was added to this powder, stirred for 12 hours, filtered to remove insoluble matters, and concentrated to obtain 16 g of a green primary extract.
  • 8 ml each of water or ethanol containing 10% to 90% water was added to 0. lg of this extract, and after sonication, the mixture was allowed to stand at 5 ° C. Centrifugation was performed at 7000 rpm for 15 minutes to separate the supernatant and the precipitate. The supernatant was used as it was, and the precipitate was dissolved in 8 ml of dimethylformamide to prepare a sample for measuring force rolls.
  • the concentration of the power roll in the sample was measured by HPLC analysis under the following conditions.
  • Fig. 5 shows the results of the power roll concentration in the sediment, and Fig. 5 shows the results of the power roll concentration in the supernatant.
  • Example 16 Production of plant extract containing force roll using 60% ethanol and 20% ethanol
  • the force roll concentration was measured by the method described in Example 15.
  • the plant extract (sample) containing force carol was the plant extract produced in Example 16.
  • the evaluation was performed according to the following procedure.
  • Normal human dermal fibroblasts were cultured by a conventional method using a normal human dermal fibroblast total kit (CA106K05, manufacturer: Cell Applications Inc. USA, import sales company: Toyobo Co., Ltd.).
  • the collagen production acceleration rate was determined by measuring a standard product with the above-mentioned ELISA kit, creating a calibration curve from the results, and determining the collagen production amount when the sample was added and the collagen production amount when no sample was added from the calibration curve.
  • the results are shown in FIG.
  • the horizontal axis represents the force roll concentration in the extract.
  • the collagen production activity (collagen production promoting effect) of human skin is more remarkable than that of the blank (ethanol) in the medium supplemented with the extract of plant (Momigasa) containing a powerful roll. It was recognized that it would increase. From this result, by applying a plant extract containing a power roll, the decline of fibroblasts with aging can be reduced, collagen production can be promoted, and the amount of collagen can be maintained. As a result, it is possible to effectively improve skin wrinkles and sagging caused by aging and exposure to ultraviolet rays.
  • the first invention of the present invention it is possible to provide an excellent active oxygen scavenger such as a plant-derived radical scavenging action and a lipid peroxidation inhibiting action.
  • the first invention of the present invention identifies an active ingredient of the active oxygen scavenging activity of a plant extract. By obtaining it as a more purified substance, it is possible to obtain a safer and more stable physiological effect. It is intended to be widely used as an active substance in cosmetics, functional foods, pharmaceuticals, etc.
  • the active oxygen scavenger of the first invention of the present invention is strong in a small amount! /, And has an active oxygen scavenging effect.
  • the active oxygen scavenger is used for foods and drinks, pharmaceuticals, cosmetics, whitening agents, fertilizers, feeds and the like. By using it as an external preparation for skin, etc., it is possible to obtain an antioxidative effect, in particular, an excellent antioxidative effect such as an effect of improving the storage stability of the product, and various disease preventive effects and antioxidant effects. Therefore, it is expected to greatly contribute to the industry.
  • the second invention of the present invention it is expected to have a cell activation action and an anti-aging action excellent in safety including a power roll, and a cosmetic / quasi-drug containing the cell activation and anti-aging ingredient as an active ingredient. It is expected to make a significant contribution to the industry because it can provide (external skin preparations, bath preparations, hair restorers, etc.), pharmaceuticals, and food and drinks.
  • the third invention of the present invention provides a method for efficiently removing chlorophyll from a plant extract containing a power roll, and greatly contributes to the cosmetics industry, pharmaceutical industry, food industry and the like. Touch with force S.
  • the collagen production promoter according to the fourth invention of the present invention is excellent in the effect of promoting collagen production and contains a safe power roll as an active ingredient. Therefore, it has the effect of sufficiently withstanding long-term use, and can greatly contribute to the cosmetics industry, pharmaceutical industry, food industry, and the like.

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Abstract

[PROBLEMS] To provide a active oxygen scavenger which has an excellent active oxygen scavenging activity, an activating agent or an anti-aging agent which has an excellent cell-activating activity and an excellent anti-aging activity, can stand long use satisfactorily and is safe, and a collagen production promoter which has a significant collagen production promoting activity, can stand long use satisfactorily, and is safe, and also provide a method for producing a plant extract having a high cacalol content by removing chlorophyll from a plant extract produced from a plant containing cacalol. [MEANS FOR SOLVING PROBLEMS] Disclosed are a active oxygen scavenger, an activating agent, an anti-aging agent and a collagen production promoter each comprising cacalol. Further disclosed is a method for producing a cacalol-containing plant extract, characterized by reducing the chlorophyll content of the cacalol-containing plant extract compared to that of the original extract by using a mixture composed of water and a hydrophilic organic solvent at a proper mixing ratio.

Description

明 細 書  Specification
力力ロールを含む活性酸素消去剤、賦活化剤、及びコラーゲン産生促進 剤  Active oxygen scavengers, activators, and collagen production promoters containing power rolls
技術分野  Technical field
[0001] 本発明の第 1の発明は、活性酸素消去作用に優れた薬剤に関する。さらに詳しくは The first invention of the present invention relates to a drug excellent in active oxygen scavenging action. More details
、力力ロールを有効成分とする活性酸素消去剤である。 It is an active oxygen scavenger having a power roll as an active ingredient.
[0002] 本発明の第 2の発明は、力力ロール又は Cacalia属植物の抽出物を含有する賦活化 剤に関するものであり、特に、皮膚繊維芽細胞を賦活化し、老化防止に有効な力カロ ール又は Cacalia属植物の抽出物を含有する化粧品、医薬部外品、医薬品、飲食品 に関するものである。  [0002] A second invention of the present invention relates to an activator containing a power roll or an extract of the genus Cacalia, and in particular, a power calorie that is effective in activating skin fibroblasts and preventing aging. And cosmetics, quasi-drugs, pharmaceuticals, and foods and drinks containing extracts of plants belonging to the genus Cacalia.
[0003] 本発明の第 3の発明は、力力ロールを含む植物抽出物の製造方法に関するもので ある。  [0003] A third invention of the present invention relates to a method for producing a plant extract containing a power roll.
[0004] 本発明の第 4の発明は、力力ロールを含む植物抽出物を有効成分として含有する コラーゲン産生促進剤に関するものである。  [0004] A fourth invention of the present invention relates to a collagen production promoter comprising a plant extract containing a power roll as an active ingredient.
背景技術  Background art
[0005] まず、第 1の発明の背景技術について説明する。  [0005] First, the background art of the first invention will be described.
[0006] 生物は、酸素を利用することによって生存に必要なエネルギーを効率的に得ている 。しかしながら、このようなエネルギー代謝のうち酸素が水に変換される過程で、中間 体として活性酸素種が生じる。  [0006] Living organisms efficiently obtain energy necessary for survival by using oxygen. However, reactive oxygen species are generated as intermediates in the process of oxygen being converted to water in such energy metabolism.
[0007] 生体内で発生する活性酸素としては、スーパーォキシドア二オンラジカル、過酸化 水素、ヒドロキシラジカル、一重項酸素が知られている。活性酸素種は、一般に化学 的反応性が高ぐ生体内で隣接する脂質や核酸、蛋白質等の成分と容易に反応し、 さまざまな疾病に繋がる酸化的障害をもたらす。活性酸素は、コラーゲン線維の架橋 や、 DNA損傷、連鎖的ラジカル発生による組織の損傷を引き起こし、皮膚や生体の 老化、炎症の惹起、心筋梗塞における平滑筋の損傷、肝障害の悪化、また脳組織の 破壊による痴呆の誘発などの原因となることが知られている。  [0007] As active oxygen generated in the living body, superoxide dione radical, hydrogen peroxide, hydroxy radical, and singlet oxygen are known. Reactive oxygen species generally react with components such as adjacent lipids, nucleic acids, and proteins in a living body that has high chemical reactivity, and cause oxidative damage that leads to various diseases. Reactive oxygen causes collagen fiber cross-linking, DNA damage, tissue damage due to chain radical generation, skin and living body aging, inflammation, smooth muscle damage in myocardial infarction, deterioration of liver damage, brain tissue It is known to cause dementia due to the destruction of.
[0008] 特に皮膚は紫外線など環境因子の刺激を直接受けるため、活性酸素が生成し易 V、器官であるから、活性酸素濃度の上昇が皮膚のしわ形成や皮膚の弾力性低下等 の老化、炎症、肌の色素沈着を起こす原因になるものと考えられている。そのため、 原因となる活性酸素を消去することは、健康で美しい肌を維持するためには重要で ある。 [0008] In particular, since the skin is directly stimulated by environmental factors such as ultraviolet rays, active oxygen is easily generated. V, because it is an organ, an increase in active oxygen concentration is thought to cause aging such as skin wrinkle formation and skin elasticity, inflammation, and skin pigmentation. Therefore, eliminating the causative active oxygen is important for maintaining healthy and beautiful skin.
[0009] このような活性酸素種の生体に対する毒性が明らかになるにつれ、これらを効率的 に消去する活性を有する活性酸素種消去剤は、生体内または食品や医薬品、農薬 等に含まれる成分の酸化的劣化の防御剤として有用であり、食品産業、特に水産加 ェ品、健康食品、栄養食品のほか、医薬品 ·農薬分野や化粧品分野において実利 的な利用が期待されているものである。  [0009] As the toxicity of such reactive oxygen species to the living body becomes clear, the reactive oxygen species scavenger having the activity of efficiently eliminating these active oxygen species is a component of components contained in the living body, foods, pharmaceuticals, agricultural chemicals, etc. It is useful as a protective agent against oxidative degradation and is expected to be used practically in the food industry, especially in the marine products, health foods, and nutritional foods, as well as in the fields of pharmaceuticals, agricultural chemicals, and cosmetics.
[0010] このような活性酸素消去作用を有する物質としては、ビタミン C、 E、カロテノイドといつ た天然物由来のもの、ジブチルヒドロキシトルエン、ブチルヒドロキシァ二ソールといつ た合成化合物が有効であることが確認されている。しかしながら、これらの物質は、 1 種または 2種の活性酸素種を消去するのみであるため、その活性酸素消去活性は十 分でない。また、一般に抗酸化剤は安定性が低ぐ化粧料などに配合した場合の安 定性に問題があり、実用性に乏しかった。  [0010] As substances having such an active oxygen scavenging action, vitamins C, E, carotenoids and natural products such as dibutylhydroxytoluene, butylhydroxybisole and synthetic compounds are effective. Has been confirmed. However, since these substances only erase one or two reactive oxygen species, their active oxygen scavenging activity is not sufficient. In general, antioxidants have a problem in stability when blended with cosmetics or the like having low stability, and have poor practicality.
[0011] 力力ロールは代表的なモミジガサ抽出物成分の一つであり、 5，6，7，8-テトラハイド 口- 3,4,5-トリメチルナフト [2，3-b]フラン- 9-オール構造を有する非ポリフエノール系の 脂溶性物質である。また、力力ロールはモミジガサ以外の植物の抽出物中にも含まれ ていることが知られている。例えば、 Cacalia  [0011] The power roll is one of the typical maple extract components, and 5,6,7,8-tetrahydrone-3,4,5-trimethylnaphtho [2,3-b] furan-9 -It is a non-polyphenolic fat-soluble substance with an all structure. In addition, it is known that power rolls are also contained in extracts from plants other than Momijigasa. For example, Cacalia
adenostyloides (非特許文献 5)、 Cacalia auriculata、非特許文献 6)、 Ligularia virgaur ea (非特許文献 7)、 Psacalium radulifolium (非特許文献 3)などが報告されている。  Adenostyloides (Non-patent document 5), Cacalia auriculata, Non-patent document 6), Ligularia virgaur ea (Non-patent document 7), Psacalium radulifolium (Non-patent document 3) and the like have been reported.
[0012] 力力ロールは、 DPPH (1 , l—Diphenyl— 2— Picrylhydrazyl)消去能（非特許文 献 1)、過酸化脂質抑制能 (非特許文献 2)などを有することが知られている。その他 の力力ロール類の生理作用としては、抗菌作用（非特許文献 3)、血糖低下作用（非 特許文献 4)が報告されている。し力もながら、生体内で発生するスーパーォキシド、 過酸化水素、一重項酸素等の活性酸素種に対する消去作用、および安定性につい てはまったく知られていなかった。  [0012] Power rolls are known to have DPPH (1, l-Diphenyl-2-picrylhydrazyl) scavenging ability (Non-patent Document 1), lipid peroxide inhibiting ability (Non-patent Document 2), etc. . As other physiological effects of force rolls, antibacterial action (Non-patent Document 3) and hypoglycemic action (Non-Patent Document 4) have been reported. However, the superoxide, hydrogen peroxide, and the scavenging action against active oxygen species such as singlet oxygen and the stability generated in the living body were not known at all.
[0013] 次に、第 2の発明の背景技術について説明する。 [0014] 個体老化やこれに伴って発生する各種の疾患などは、分裂するすべての細胞の老 化（分裂速度の低下、細胞機能の低下）と深く関わっている。例えば皮膚は、表皮細 胞、繊維芽細胞、およびこれら細胞外の皮膚構造を支持するエラスチン、コラーゲン 等の細胞外マトリックスによって構成されている。若い皮膚においては、これらの皮膚 組織の相互作用が恒常性を保つことによって水分保持、柔軟性、弾力性が確保され 、肌は外観的にも張りと艷があり、みずみずしい状態に維持される。ところ力 加齢や 紫外線、乾燥、ストレスなどによって特に細胞外マトリックスや繊維芽細胞の機能低下 力引き起こされ、その結果、皮膚の柔軟性、保湿機能は低下し、肌は張りゃ艷を失い 、荒れ、しわ、くすみなどの老化症状が発生する。 Next, the background art of the second invention will be described. [0014] Individual aging and the various diseases that accompany this are closely related to the aging of all cells that divide (decrease in the division rate and cell function). For example, the skin is composed of epidermal cells, fibroblasts, and extracellular matrices such as elastin and collagen that support these extracellular skin structures. In young skin, moisture retention, flexibility, and elasticity are ensured by maintaining the homeostasis of the interaction between these skin tissues, and the skin has a firmness and wrinkles in appearance, and is maintained in a fresh state. However, due to aging, ultraviolet rays, drying, stress, etc., the extracellular matrix and fibroblast function are particularly weakened. As a result, the skin's flexibility and moisturizing function are reduced, and the skin loses its habit and becomes rough. Aging symptoms such as wrinkles and dullness occur.
[0015] 細胞レベルで老化防止や抑制を目的に賦活化剤、抗老化剤の探索が行われて!/、 る。例えば動物由来の賦活化剤としては、結合組織加水分解物（特許文献 1)、胸腺 •脾臓由来水溶性蛋白（特許文献 2)、牛胎盤エキス（特許文献 3)などが知られて!/ヽ る。植物由来の賦活化剤としては、ゴマ、サンャク、トウガラシ、トウキ、ドクダミ、バクモ ンドウ（特許文献 4)、アーモンド、セィヨウタンポポ、セィヨウニヮトコ、センキユウ、セン ブリ、ソゥハクヒ、トウニン、ニンジン、ホップ、ムクゲ、ョクイニン（特許文献 5)、ショウガ 科ゥコン属（特許文献 6)、ハナヤスリ科ハナヤスリ属（特許文献 7)の抽出物などが知 られている。これらの一部は賦活化剤、抗老化剤として医薬部外品や化粧品に利用 されているが、満足すべき作用効果を発揮する賦活化剤ゃ抗老化剤は得られていな い。  [0015] At the cellular level, searching for activators and anti-aging agents has been carried out for the purpose of preventing or suppressing aging! For example, as an animal-derived activator, connective tissue hydrolyzate (Patent Document 1), thymus • spleen-derived water-soluble protein (Patent Document 2), bovine placenta extract (Patent Document 3), etc. are known! / ヽThe Examples of plant-derived activators include sesame seeds, peonies, chili peppers, toki, dokudami, bakumondo (Patent Document 4), almonds, dandelion, seitonito, sensyuyu, assembly, sohakhi, tonin, carrot, hop, mukuge, Extracts of gyokuinin (patent document 5), ginger family turmeric genus (patent document 6), and blossom genus Hanayasuri (patent document 7) are known. Some of these are used in quasi-drugs and cosmetics as activators and anti-aging agents, but no activators that exhibit satisfactory effects have been obtained.
[0016] 植物二次代謝物には、ポリフエノール等の植物色素の他に精油、呈味成分、タン二 ン、アルカロイド等が知られている。「力力ロール」は代表的なモミジガサ抽出物成分 の一つであり、 5, 6 , 7, 8 テトラノヽィドロ一 3, 4, 5 トリメチノレナフト [2, 3— b]フラ ンー 9 オール構造を有する非ポリフエノール系（ノンフラボノイド系）の植物二次代 謝物である。また、力力ロールはモミジガサ以外の植物の抽出物中にも含まれている ことカ失口られてレヽる。 ί列え (ま、、 Cacalia adenostyloides ( 特許文献 5)、 Cacalia auricul ata (非特許文献 6)、 Ligularia  [0016] As plant secondary metabolites, essential oils, taste components, tannin, alkaloids and the like are known in addition to plant pigments such as polyphenol. “Strength roll” is one of the typical maple extract components. 5, 6, 7, 8 Tetranoidro 3, 4, 5 Trimethinorenaft [2, 3— b] furan 9 o It is a non-polyphenol (non-flavonoid) plant secondary metabolite with a structure. The power roll is also included in the extracts of plants other than Momijigasa, and it is lost. ί 列 (Ma, Cacalia adenostyloides (Patent Document 5), Cacalia auricul ata (Non-Patent Document 6), Ligularia
virgaurea (非特許文献 7)、 Psacalium radulifolium (非特許文献 3)などが報告されて いる。 [0017] モミジガサ（Cacalia delphiniifolia Sieb et Zucc)は、別名、シドケ、シドキ、シドキナ、 シンドキ、キノシタ、トウキチ、トウキチナ、スドケ、シトギ、スドケと呼ばれており、キク科 の植物で日本国内に広く分布し、山菜として食されている。モミジガサ抽出物には美 白作用があることが知られている（特許文献 8)。モミジガサの主成分の一つに力カロ ールが存在している。力力ロールは脂質過酸化を抑制する、優れた抗酸化能を有し ていることが知られている（非特許文献 2)。力力ロール類の生理作用としては、抗菌 作用（非特許文献 3)、血糖低下作用（非特許文献 4)が報告されてレ、る。しかし、力力 ロールの細胞に対する賦活化作用ゃ抗老化作用については全く知られていなかつ た。 virgaurea (Non-patent document 7), Psacalium radulifolium (Non-patent document 3) and the like have been reported. [0017] Momijigasa (Cacalia delphiniifolia Sieb et Zucc) is also known as Sidke, Sidki, Sidkina, Shindoki, Kinoshita, Toukichi, Tokichina, Sudoke, Sitgi, Sudoke, and is widely distributed in Japan. It is eaten as a wild vegetable. Momijigasa extract is known to have a whitening effect (Patent Document 8). One of the main components of Momijigasa is force carol. It is known that a powerful roll has excellent antioxidant ability that suppresses lipid peroxidation (Non-patent Document 2). As the physiological action of force rolls, antibacterial action (Non-patent Document 3) and hypoglycemic action (Non-Patent Document 4) have been reported. However, the activation effect of the force roll on cells and the anti-aging effect were not known at all.
[0018] 次に、第 3および第 4の発明の背景技術について説明する。  Next, background art of the third and fourth inventions will be described.
[0019] 個体老化やこれに伴って発生する各種の疾患などは、分裂するすべての細胞の老 化（分裂速度の低下、細胞機能の低下）と深く関わっている。例えば皮膚 (真皮およ び表皮）は、表皮細胞、繊維芽細胞、およびこれら細胞外の皮膚構造を支持するェ ラスチン、コラーゲン等の細胞外マトリックスによって構成されている。若い皮膚にお いては、これらの皮膚組織の相互作用が恒常性を保つことによって水分保持、柔軟 性、弾力性が確保され、肌は外観的にも張りと艷があり、みずみずしい状態に維持さ れる。ところカ、加齢や紫外線、乾燥、ストレスなどによって特に細胞外マトリックスや 繊維芽細胞の機能低下が引き起こされ、その結果、皮膚の柔軟性、保湿機能は低下 し、肌は張りゃ艷を失い、荒れ、しわ、くすみなどの老化症状が発生する。  [0019] Individual aging and the various diseases that accompany this are closely related to the aging of all cells that divide (decrease in division rate and cell function). For example, the skin (dermis and epidermis) is composed of epidermal cells, fibroblasts, and extracellular matrices such as elastin and collagen that support these extracellular skin structures. In young skin, the interaction of these skin tissues maintains homeostasis, ensuring moisture retention, flexibility, and elasticity, and the skin is maintained in a fresh state with tension and wrinkles. It is. However, aging, UV rays, dryness, stress, etc. cause a decrease in the function of the extracellular matrix and fibroblasts in particular, resulting in a decrease in skin flexibility and moisturizing function, and the skin loses its habits. Aging symptoms such as roughening, wrinkles and dullness occur.
[0020] 細胞レベルで老化防止や抑制を目的に賦活化剤、抗老化剤の探索が行われて!/、 る。例えば動物系由来の賦活化剤としては、結合組織加水分解物（特許文献 1)、胸 腺 ·脾臓由来水溶性蛋白（特許文献 2)、牛胎盤エキス（特許文献 3)などが知られて いる。植物系由来の賦活化剤としては、ゴマ、サンャク、トウガラシ、トウキ、ドクダミ、 バタモンドウ（特許文献 4)、アーモンド、セィヨウタンポポ、セィヨウニヮトコ、センキュ ゥ、センブリ、ソゥハクヒ、トウニン、ニンジン、ホップ、ムクゲ、ョクイニン（特許文献 5)、 ショウガ科ゥコン属（特許文献 6)、ハナヤスリ科ハナヤスリ属（特許文献 7)の抽出物 などが知られている。 [0020] At the cellular level, searching for activators and anti-aging agents has been carried out for the purpose of preventing or inhibiting aging! For example, connective tissue hydrolysates (patent document 1), thymus / spleen-derived water-soluble protein (patent document 2), bovine placenta extract (patent document 3), etc. are known as animal-derived activators. . Examples of plant-derived activators include sesame seeds, peonies, capsicum, toki, dokudami, batamando (patent document 4), almonds, dandelion, seitonitoko, senkyu, assembly, sohakhi, tonin, carrot, hop, mukuge, Extracts of gyokuinin (patent document 5), ginger family turmeric genus (patent document 6), and genus genus genus (patent document 7) are known.
[0021] 植物二次代謝物には、ポリフエノール等の植物色素の他に精油、呈味成分、タン二 ン、アルカロイド等が知られている。本発明で言うところの「力力ロール」は代表的なモ ミジガサ抽出物成分の一つであり、 5, 6, 7, 8—テトラハイドロー 3, 4, 5—トリメチル ナフト [2, 3— b]フラン 9 オール構造を有する非ポリフエノール系（ノンフラボノィ ド系）の植物二次代謝物である。また、力力ロールはモミジガサ以外の植物の抽出物 中にも含まれていることが知られている。例えば、 Cacalia adenostyloides (非特許文 献 5)、 Cacalia auriculata (非特許文献 6)、 Ligularia [0021] In addition to plant pigments such as polyphenols, plant secondary metabolites include essential oils, taste components, And alkaloids are known. The “power roll” as used in the present invention is one of typical maple extract components, and includes 5, 6, 7, 8-tetrahydro 3, 4, 5-trimethyl naphtho [2, 3— b] Non-polyphenolic (non-flavonoid) plant secondary metabolite with furan 9-ol structure. In addition, it is known that power rolls are also contained in extracts from plants other than Momijigasa. For example, Cacalia adenostyloides (Non-Patent Document 5), Cacalia auriculata (Non-Patent Document 6), Ligularia
virgaurea (非特許文献 7)、 Psacalium radulifolium (非特許文献 3)などが報告されて いる。 （発明 2より追加引用）  virgaurea (Non-patent document 7), Psacalium radulifolium (Non-patent document 3) and the like have been reported. (Additional quotation from Invention 2)
[0022] モミジガサは、別名、シドケ、シドキ、シドキナ、シンドキ、キノシタ、トウキチ、トウキチ ナ、スドケ、シトギ、スドケと呼ばれており、キク科の植物で国内に広く分布し山菜とし て食されている。モミジガサ抽出物には美白作用が知られている（特許文献 8)。モミ ジガサ抽出物の主成分の一つに力力ロールが存在しており、力力ロールは脂質過酸 化を抑制する優れた抗酸化能を有して!/、ることが知られて!/、る（非特許文献 2)。力力 ロール類の生理作用としては、抗菌作用（非特許文献 3)、血糖低下作用（非特許文 献 4)が報告されている。  [0022] Momijigasa is also known as Sidke, Sidki, Sidkina, Shindoki, Kinoshita, Toukichi, Tokichina, Sudoke, Sitogi, Sudoke, and is widely distributed in the country and is eaten as a wild vegetable. Yes. A whitening effect is known for the extract of Japanese maple (Patent Document 8). A powerful roll exists as one of the main components of the extract of Momijigasa, and it is known that the powerful roll has an excellent antioxidant ability to suppress lipid peroxidation! /, Ru (non-patent document 2). As physiological effects of force rolls, antibacterial action (Non-patent Document 3) and hypoglycemic action (Non-Patent Document 4) have been reported.
[0023] ここで、植物を有機溶媒等で抽出した抽出物は、光合成生物特有の色素であるク ロロフィルが多量に含有されていることが多い。クロロフィルは、化学的'酵素的にフエ オフオルバイド等の分解物に容易に変換される。このクロロフィル分解物は、太陽光 によって皮膚等の炎症を引き起こす光過敏症原因物質の一つであるため、クロロフィ ルおよびその分解物を抽出物から除去する必要がある（非特許文献 8)。また、クロ口 フィル系色素を多く含んだ植物抽出物は、緑色が強く商品価値が低いば力、りでなぐ 日光、蛍光灯などの光による保存中の劣化を受けやすいため、このクロロイル系色素 をより経済的に、し力、も極力除去することが重要な技術であった。しかるに、これまで 知られているモミジガサ抽出物はメタノール等の有機溶媒で単に抽出後、減圧濃縮 したものであり（特許文献 8)、クロロフィルの濃度を下げ、力力ロール濃度を高めるよう な方法はこれまでまったく知られていなかった。  [0023] Here, an extract obtained by extracting a plant with an organic solvent or the like often contains a large amount of chlorophyll, which is a pigment unique to photosynthetic organisms. Chlorophyll is readily converted chemically and enzymatically into degradation products such as pheophorbide. Since this chlorophyll degradation product is one of the substances causing photosensitivity that causes inflammation of the skin and the like by sunlight, it is necessary to remove chlorophyll and its degradation products from the extract (Non-patent Document 8). In addition, plant extracts that contain a lot of black-filled pigments are more susceptible to deterioration during storage due to sunlight, fluorescent light, etc. It was an important technology to remove the power as much as possible, more economically. However, the known Japanese maple extract is simply extracted with an organic solvent such as methanol and then concentrated under reduced pressure (Patent Document 8). There is no method for reducing the chlorophyll concentration and increasing the power roll concentration. It has never been known before.
[0024] 一方、細胞外マトリックス産生促進作用を有する物質としては、例えばコラーゲンを 産生促進する作用を有する、甘草葉抽出物 (特許文献 9)、ハス胚芽抽出物 (特許文 献 10)、加水分解バレイショタンパク（特許文献 11)、柿の葉抽出物またはサンザシ の果実抽出物（特許文献 12)などが知られており、これらの一部はコラーゲン産生促 進剤として医薬部外品や化粧品への応用が試みられているが、作用効果が十分とは 言えず、満足すべき作用効果を発揮する細胞外マトリックス産生促進剤は得られて いなかった。 [0024] On the other hand, as a substance having an extracellular matrix production promoting action, for example, a licorice leaf extract (patent document 9), a lotus germ extract (patent text) having an action of promoting collagen production. 10), hydrolyzed potato protein (Patent Document 11), bamboo leaf extract or hawthorn fruit extract (Patent Document 12), etc., some of which are used as a collagen production promoter in the pharmaceutical department. Although application to external products and cosmetics has been attempted, it cannot be said that the effect is sufficient, and an extracellular matrix production promoter that exhibits a satisfactory effect has not been obtained.
特許文献 1：特開昭 62— 84024号公報 Patent Document 1: Japanese Patent Laid-Open No. 62-84024
特許文献 2：特開昭 63— 188697号公報 Patent Document 2: JP-A 63-188697
特許文献 3：特開平 03— 141299号公報 Patent Document 3: Japanese Patent Laid-Open No. 03-141299
特許文献 4 :特開平 10— 45615号公報 Patent Document 4: Japanese Patent Laid-Open No. 10-45615
特許文献 5 :特開平 10— 36279号公報 Patent Document 5: Japanese Patent Laid-Open No. 10-36279
特許文献 6：特開 2004— 75632号公幸 Patent Document 6: Japanese Patent Publication No. 2004-75632
特許文献 7：特開 2005— 89375号公報 Patent Document 7: JP-A-2005-89375
特許文献 8：特開開平 05— 345705号公報 Patent Document 8: Japanese Unexamined Patent Publication No. 05-345705
特許文献 9 :特開 2000— 191498号公報 Patent Document 9: Japanese Unexamined Patent Publication No. 2000-191498
特許文献 10 :特開 2002— 29980号公報 Patent Document 10: Japanese Unexamined Patent Application Publication No. 2002-29980
特許文献 11 :特開 2005— 263689号公報 Patent Document 11: Japanese Unexamined Patent Publication No. 2005-263689
特許文献 12：特開 2006— 160629号公報 Patent Document 12: Japanese Unexamined Patent Publication No. 2006-160629
非特許文献 l : Revista de la sociedad Quimica de Mexico., 47, 160(2003) 非特許文献 2 : Bioscience Biotechnology and Biochemistry, 68， 1393(2004) 非特許文献 3 : Journal of Natural Products, 64， 432(2001) Non-patent literature l: Revista de la sociedad Quimica de Mexico., 47, 160 (2003) Non-patent literature 2: Bioscience Biotechnology and Biochemistry, 68, 1393 (2004) Non-patent literature 3: Journal of Natural Products, 64, 432 ( 2001)
非特許文献 4 : Journal of Natural Products, 62， 1088(1999) Non-Patent Document 4: Journal of Natural Products, 62, 1088 (1999)
非特許文献 5 : Chemical& Pharmaceutical Bulletin, 33， 4792(1985) Non-Patent Document 5: Chemical & Pharmaceutical Bulletin, 33, 4792 (1985)
非特許文献 6 : Khimiya Prirodnykh Soedienli, 5, 643(1989) Non-Patent Document 6: Khimiya Prirodnykh Soedienli, 5, 643 (1989)
非特許文献 7 : Phytochemistry, 30， 3132 (1991) Non-Patent Document 7: Phytochemistry, 30, 3132 (1991)
非特許文献 8 : Food& Food Ingredients J. Jpn, 172， 29(1997) Non-Patent Document 8: Food & Food Ingredients J. Jpn, 172, 29 (1997)
図面の簡単な説明 Brief Description of Drawings
[図 1]過酸化水素消去試験の結果  [Figure 1] Results of hydrogen peroxide elimination test
園 2]—重項酸素消去試験の結果 [図 3]安定性試験の結果 2] —Results of the triplet oxygen scavenging test [Figure 3] Results of stability test
[図 4]正常ヒト皮膚繊維芽細胞を用いたモミジガサ抽出物の細胞賦活作用を検討した  [Fig.4] Cell activation of Momijigasa extract using normal human skin fibroblasts was examined.
[図 5]エタノール含水率を変えたときの沈殿物中の力力ロール量を示す図である。 FIG. 5 is a diagram showing the amount of power rolls in the precipitate when the water content of ethanol is changed.
[図 6]エタノール含水率を変えたときの上清中の力力ロール量を示す図である。  FIG. 6 is a diagram showing the amount of power roll in the supernatant when the water content of ethanol is changed.
[図 7]実施例 2の製造スキームを示す図である。  FIG. 7 shows a production scheme of Example 2.
[図 8]力力ロールを含む植物抽出物による正常ヒト皮膚線維芽細胞に対するコラーゲ ン産生促進作用を確認した結果を示す図である。  FIG. 8 shows the results of confirming the collagen production promoting action on normal human skin fibroblasts by a plant extract containing a power roll.
発明の開示  Disclosure of the invention
発明が解決しょうとする課題  Problems to be solved by the invention
[0026] 本発明の第 1の発明は、従来の問題点を解決し、活性酸素消去作用に優れた活性 酸素消去剤を提供し、安定性の高い外用剤を提供することを目的とする。  [0026] The first invention of the present invention is to solve the conventional problems, to provide an active oxygen scavenger excellent in active oxygen scavenging action, and to provide a highly stable external preparation.
[0027] 本発明の第 2の発明は次の通りである。  [0027] The second invention of the present invention is as follows.
哺乳類、特にヒトに対して賦活化ゃ抗老化を付与することは極めて重要な課題であり 、動物由来、植物由来の賦活物質ゃ抗老化剤は各種見つかつている力 実際には 産業上利用可能な程度に十分かつ安定した効果は得られておらず、新規な賦活化 剤ゃ抗老化剤が探索されているのが現状である。従って、本発明の第 2の発明の目 的は、細胞に対する賦活化および抗老化効果に優れ、長期にわたる使用に十分に 耐え得る安全性を備えた賦活化剤および抗老化剤を提供し、これらを有効成分とし た化粧品 ·医薬部外品、医薬品、飲食品を提供することである。  Giving activation to mammals, especially humans, is an extremely important issue, and animal-derived and plant-derived activation substances have various potentials. Actually, they can be used industrially. A sufficient and stable effect has not been obtained, and a new activator or anti-aging agent is currently being searched. Therefore, the purpose of the second invention of the present invention is to provide an activator and an anti-aging agent that are excellent in activation and anti-aging effects on cells, and have safety sufficient to withstand long-term use. To provide cosmetics and quasi-drugs, pharmaceuticals, and foods and drinks.
[0028] 本発明の第 3および第 4の発明は次の通りである。  [0028] The third and fourth aspects of the present invention are as follows.
上述のように、これまで知られているモミジガサ抽出物はメタノール等の有機溶媒で 単に抽出後、減圧濃縮したものであり（特許文献 8)、クロロフィルの濃度を下げ、力力 ロール濃度を高めるような方法はこれまでまったく知られていなかった。  As described above, the known Japanese maple extract is simply extracted with an organic solvent such as methanol and concentrated under reduced pressure (Patent Document 8). The concentration of chlorophyll is lowered to increase the strength roll concentration. This method has never been known.
[0029] また、従来のコラーゲン産生促進物質は作用効果が十分とは言えず、満足すべき 作用効果を発揮する新たなコラーゲン産生促進物質が強く望まれていた。  [0029] In addition, the conventional collagen production promoting substance cannot be said to have a sufficient effect, and a new collagen production promoting substance that exhibits a satisfactory effect has been strongly desired.
[0030] 本発明は、上記の問題点に鑑みてなされたものであり、その目的は、力力ロールを 含む植物から得られる植物抽出物中のクロロフィルを除去し、力力ロール濃度の高い 植物抽出物の製造方法を提供すること、並びに、顕著なコラーゲン産生促進作用を 有すると同時に、長期にわたる使用に十分に耐え得る安全性を備えたコラーゲン産 生促進剤を提供することである。 [0030] The present invention has been made in view of the above-mentioned problems, and its purpose is to remove chlorophyll from a plant extract obtained from a plant containing a power roll and to increase the power roll concentration. Another object is to provide a method for producing a plant extract, and to provide a collagen production promoter that has a remarkable collagen production promoting action and at the same time has sufficient safety to withstand long-term use.
課題を解決するための手段  Means for solving the problem
[0031] 本発明者らは、上記の課題に鑑み鋭意研究を進めた結果、力力ロールが、スーパ ーォキシド、過酸化水素、および一重項酸素のいずれの活性酸素種に対しても消去 作用を有すること、および力力ロールが優れた安定性を有することを見出し、本発明 の第 1の発明を完成したものである。すなわち、本発明の第 1の発明は、以下のような 構成からなる。 [0031] As a result of diligent research in view of the above problems, the present inventors have found that the power roll has an erasing action on any active oxygen species such as superoxide, hydrogen peroxide, and singlet oxygen. And having found that the power roll has excellent stability, the first invention of the present invention has been completed. That is, the first invention of the present invention has the following configuration.
[0032] (1 - 1 )力力ロールを含有することを特徴とする活性酸素消去剤。  [0032] (1-1) An active oxygen scavenger characterized by containing a force roll.
[0033] (1 - 2)力力ロールを有効成分として含有し、 [0033] (1-2) containing a force roll as an active ingredient,
スーパーォキシド、過酸化水素、および一重項酸素からなる群から選択される少なく とも 1種以上の活性酸素を消去することを特徴とする（1 1)の活性酸素消去剤。  (11) The active oxygen scavenger characterized by eliminating at least one active oxygen selected from the group consisting of superoxide, hydrogen peroxide, and singlet oxygen.
[0034] (1 - 3) (1 1)または（1 2)の力カロールカ Cacalia属植物から抽出された力カロ ールであることを特徴とする活性酸素消去剤。 [0034] (1-3) A reactive oxygen scavenger characterized by being a power carol extracted from a plant of the genus Cacalia of (1 1) or (1 2).
[0035] (1 -4) (1 3)の Cacalia属植物が、モミジガサであることを特徴とする活性酸素消 去剤。 [0035] (1 -4) An active oxygen scavenger, wherein the Cacalia plant of (1 3) is a maple grass.
[0036] (1 - 5) (1 1)または（1 2)の力カロールカ S、化学合成により製造された力カロー ルであることを特徴とする活性酸素消去剤。  [0036] (1-5) (1 1) or (1 2) force Karolka S, an active oxygen scavenger characterized by being a force carol produced by chemical synthesis.
[0037] (1 - 6) (1一；!）〜（1 5)のいずれかの活性酸素消去剤を用いて、 [0037] (1-6) (1;;) using any one of active oxygen scavengers of (1 5),
スーパーォキシド、過酸化水素、および一重項酸素からなる群から選択される少なく とも 1種以上の活性酸素を消去することを特徴とする活性酸素消去方法。  A reactive oxygen scavenging method comprising scavenging at least one active oxygen selected from the group consisting of superoxide, hydrogen peroxide, and singlet oxygen.
[0038] (1 - 7) (1一；!）〜（1 5)のいずれかの活性酸素消去剤を有効成分として含有する 化粧品類。 [0038] (1-7) Cosmetics containing the active oxygen scavenger according to any one of (1 ;!) to (15) as an active ingredient.
[0039] (1 - 8) (1一；!）〜（1 5)のいずれかの活性酸素消去剤を有効成分として含有する 医薬部外品類。  [0039] (1-8) A quasi-drug containing an active oxygen scavenger according to any one of (1 ;!) to (15) as an active ingredient.
[0040] (1 - 9) (1一；!）〜（1 5)のいずれかの活性酸素消去剤を有効成分として含有する 飲食品類。 [0041] (1 - 10) (1一；!）〜（1 5)のいずれかの活性酸素消去剤を有効成分として含有す る医薬品類。 [0040] (1-9) Food / beverage products containing the active oxygen scavenger of any one of (1 ;!) to (15) as an active ingredient. [0041] (1-10) A pharmaceutical product comprising the active oxygen scavenger of any one of (1 ;!) to (15) as an active ingredient.
[0042] さらに、 [0042] Furthermore,
(1 - 11)力力ロールを有効成分として含有し、  (1-11) contains a power roll as an active ingredient,
スーパーォキシド、過酸化水素、および一重項酸素からなる群から選択される少なく とも 2種以上の活性酸素を消去することを特徴とする（1 1)の活性酸素消去剤。  The active oxygen scavenger according to (11), wherein at least two kinds of active oxygen selected from the group consisting of superoxide, hydrogen peroxide, and singlet oxygen are eliminated.
[0043] (1 - 12)力力ロールを有効成分として含有し、 [0043] (1-12) containing a force roll as an active ingredient,
スーパーォキシド、過酸化水素、および一重項酸素を消去することを特徴とする（1 It is characterized by eliminating superoxide, hydrogen peroxide, and singlet oxygen (1
1)の活性酸素消去剤。 1) Active oxygen scavenger.
[0044] さらに、 [0044] In addition,
(1 - 13) (1一；!）〜（1 5)の!/、ずれかの活性酸素消去剤を用いて、  (1-13) (1;;) to (15)! /, Using any active oxygen scavenger,
スーパーォキシド、過酸化水素、および一重項酸素からなる群から選択される少なく とも 2種以上の活性酸素を消去することを特徴とする活性酸素消去方法。  A reactive oxygen scavenging method comprising scavenging at least two kinds of active oxygen selected from the group consisting of superoxide, hydrogen peroxide, and singlet oxygen.
[0045] (1 - 14) (1一;!）〜（1 5)のいずれかの活性酸素消去剤を用いて、 [0045] (1-14) (1 1 ;!) to any one of (1 5) active oxygen scavenger,
スーパーォキシド、過酸化水素、および一重項酸素を消去することを特徴とする活性 酸素消去方法。  An active oxygen scavenging method characterized by scavenging superoxide, hydrogen peroxide, and singlet oxygen.
[0046] 本発明者らは上記の目的を達成すべく鋭意努力した結果、モミジガサ抽出物が細 胞の賦活化に有効であることを見出し、本発明の第 2の発明を完成させるに至った。 すなわち、本発明の第 2の発明は、以下の発明を包含する。  [0046] As a result of diligent efforts to achieve the above-mentioned object, the present inventors have found that a maple extract is effective in activating cells, and have completed the second invention of the present invention. . That is, the second invention of the present invention includes the following inventions.
[0047] (2- 1)キク科植物の抽出物を有効成分として含有することを特徴とする賦活化剤。 [0047] (2-1) An activator comprising an extract of an Asteraceae plant as an active ingredient.
[0048] (2- 2) 上記キク科植物の抽出物が力力ロールを含むことを特徴とする（2— 1)に記 載の賦活化剤。 [0048] (2-2) The activator according to (2-1), wherein the extract of the Asteraceae plant contains a power roll.
[0049] (2- 3) 上記キク科植物がコゥモリソゥ属植物であることを特徴とする（2— 1)または（ [0049] (2-3) The asteraceae plant is a plant belonging to the genus Batrizo (2-1) or (
2— 2)に記載の賦活化剤。 2—Activator according to 2).
[0050] (2-4) 上記コゥモリソゥ属植物がモミジガサであることを特徴とする（2— 3)に記載 の賦活化剤。 [0050] (2-4) The activator according to (2-3), wherein the plant of the genus Batridae is Momijigasa.
[0051] (2- 5)力力ロールを有効成分として含有することを特徴とする賦活化剤。  [0051] (2-5) An activator comprising a power roll as an active ingredient.
[0052] (2- 6) 上記（2—；!）〜（2— 5)のいずれ力、 1項に記載の賦活化剤を有効成分として 含有することを特徴とする抗老化剤。 [0052] (2-6) Any force of (2— ;!) to (2-5) above, with the activator according to item 1 as an active ingredient An anti-aging agent characterized by containing.
[0053] (2- 7) 上記（2—；!）〜（2— 5)のいずれ力、 1項に記載の賦活化剤、または（2— 6)に 記載の抗老化剤を有効成分として含有することを特徴とする外用剤。 [0053] (2-7) Any of the above (2— ;!) to (2-5), the activator according to item 1, or the anti-aging agent according to (2-6) as an active ingredient An external preparation characterized by containing.
[0054] (2- 8) 上記（2—；!）〜（2— 5)のいずれ力、 1項に記載の賦活化剤、または（2— 6)に 記載の抗老化剤を有効成分として含有することを特徴とする化粧品。 [0054] (2-8) As an active ingredient, any one of the above (2— ;!) to (2-5), the activator according to item 1, or the anti-aging agent according to (2-6) Cosmetics characterized by containing.
[0055] (2- 9) 上記（2—；!）〜（2— 5)のいずれ力、 1項に記載の賦活化剤、または（2— 6)に 記載の抗老化剤を有効成分として含有することを特徴とする医薬部外品。 [0055] (2-9) As an active ingredient, any one of the above (2— ;!) to (2-5), the activator according to item 1, or the anti-aging agent according to (2-6) A quasi-drug characterized by containing.
[0056] (2- 10) 上記（2—；!）〜（2— 5)のいずれ力、 1項に記載の賦活化剤、または（2— 6) に記載の抗老化剤を有効成分として含有することを特徴とする医薬品。 [0056] (2-10) As an active ingredient, any of the above (2— ;!) to (2-5), the activator according to 1 or the anti-aging agent according to (2-6) A pharmaceutical product characterized by containing.
[0057] (2- 11) 上記（2—；!）〜（2— 5)のいずれ力、 1項に記載の賦活化剤、または（2— 6) に記載の抗老化剤を有効成分として含有することを特徴とする飲食品。 [0057] (2-11) As an active ingredient, any one of the above (2— ;!) to (2-5), the activator according to item 1, or the anti-aging agent according to (2-6) A food or drink characterized by containing.
[0058] 本発明者らは上記の目的を達成すべく鋭意努力した結果、適切な溶媒を用いて力 力ロールを含む植物抽出物から得られる二次抽出物は、元の力力ロールを含む植物 抽出物よりクロロフィル含量が減少していること（第 3の発明）を見出し、さらに、力カロ ールを含む植物抽出物がコラーゲン産生促進作用を有すること（第 4の発明）を見出 し、本発明の第 3および第 4の発明を完成させるに至った。すなわち、本発明の第 3 および第 4の発明は、以下の発明を包含する。 [0058] As a result of diligent efforts to achieve the above object, the present inventors have obtained a secondary extract obtained from a plant extract containing a power roll using an appropriate solvent, which contains the original power roll. It was found that the chlorophyll content was lower than that of the plant extract (third invention), and further, the plant extract containing strong calorie was found to have a collagen production promoting action (fourth invention). The present inventors have completed the third and fourth inventions of the present invention. That is, the third and fourth inventions of the present invention include the following inventions.
[0059] 本発明の第 3の発明は、以下の発明を包含する。 [0059] The third invention of the present invention includes the following inventions.
[0060] (3- 1)力力ロールを含む植物抽出物から水および親水性有機溶媒の混合物を用 いて二次抽出物を得る工程を包含し、上記二次抽出物は上記力力ロールを含む植 物抽出物と比較してクロロフィル含量が減少していることを特徴とする力力ロールを含 む植物抽出物の製造方法。  (3-1) The method includes a step of obtaining a secondary extract from a plant extract containing a power roll using a mixture of water and a hydrophilic organic solvent, and the secondary extract includes the power roll. A method for producing a plant extract containing a power roll, wherein the chlorophyll content is reduced as compared with a plant extract containing the same.
[0061] (3- 2) 上記二次抽出物中のクロロフィル含量が目的の数 以下になるまで上記 工程を繰り返すことを特徴とする（3— 1)に記載の力力ロールを含む植物抽出物の製 造方法。  [0061] (3- 2) The plant extract containing a power roll according to (3-1), characterized in that the above steps are repeated until the content of chlorophyll in the secondary extract is below the desired number. Manufacturing method.
[0062] (3- 3)さらに、力力ロールを含む植物抽出物から、水、または、水および親水性有 機溶媒の混合物を用いて力力ロールより高親水性の夾雑物を除去する工程を包含 することを特徴とする（3— 1)または（3— 2)に記載の力力ロールを含む植物抽出物 の製造方法。 (3-3) Further, a step of removing impurities having higher hydrophilicity than the power roll from the plant extract containing the power roll using water or a mixture of water and a hydrophilic organic solvent. A plant extract containing a power roll according to (3-1) or (3-2), characterized in that Manufacturing method.
[0063] (3-4) 上記親水性有機溶媒が、エタノール、アセトン、イソプロピルアルコール、 プロピレングリコール、ブチレングリコール、およびブタノールからなる群より選ばれる [0063] (3-4) The hydrophilic organic solvent is selected from the group consisting of ethanol, acetone, isopropyl alcohol, propylene glycol, butylene glycol, and butanol.
1種または 2種以上の混合物であることを特徴とする（3—；!）〜（3— 3)のいずれかに 記載の力力ロールを含む植物抽出物の製造方法。 The method for producing a plant extract containing a power roll according to any one of (3— ;!) to (3-3), which is one or a mixture of two or more.
[0064] (3- 5) 上記二次抽出物を得る工程において用いられる水および親水性有機溶媒 の混合物が、エタノール含量 45%以上の含水エタノールであることを特徴とする（3[0064] (3-5) The mixture of water and a hydrophilic organic solvent used in the step of obtaining the secondary extract is hydrous ethanol having an ethanol content of 45% or more (3
- 1)に記載の力力ロールを含む植物抽出物の製造方法。 -The manufacturing method of the plant extract containing the power roll as described in 1).
[0065] (3- 6) 上記夾雑物を除去する工程において用いられる水および親水性有機溶媒 の混合物が、エタノール含量 40%以下の含水エタノールであることを特徴とする（3 3)に記載の力力ロールを含む植物抽出物の製造方法。 [0065] (3-6) The mixture of water and a hydrophilic organic solvent used in the step of removing impurities is hydrous ethanol having an ethanol content of 40% or less. A method for producing a plant extract containing a power roll.
[0066] (3- 7) 上記植物がキク科植物であることを特徴とする（3—；!）〜（3— 6)のいずれ かに記載の力カロールを含む植物抽出物の製造方法。 [0066] (3-7) The method for producing a plant extract containing a force carol according to any one of (3— ;!) to (3-6), wherein the plant is an Asteraceae plant.
[0067] (3- 8) 上記キク科植物がコゥモリソゥ属植物であることを特徴とする（3— 7)に記 載の力力ロールを含む植物抽出物の製造方法。 [0067] (3-8) The method for producing a plant extract containing a power roll as described in (3-7), wherein the Asteraceae plant is a plant belonging to the genus Batsoli.
[0068] (3- 9) 上記コゥモリソゥ属植物がモミジガサであることを特徴とする（3— 8)に記載 の力力ロールを含む植物抽出物の製造方法。 [0068] (3-9) The method for producing a plant extract containing a power roll according to (3-8), wherein the plant of the genus Batridae is Momijigasa.
[0069] 本発明の第 4の発明は、以下の発明を包含する。 [0069] The fourth invention of the present invention includes the following inventions.
[0070] (4- 10)力力ロールを含む植物抽出物を有効成分として含有するコラーゲン産生 促進剤。  [0070] (4-10) A collagen production promoter containing a plant extract containing a powerful roll as an active ingredient.
[0071] (4 11)力力ロールを有効成分として含有するコラーゲン産生促進剤。  [0071] (411) A collagen production promoter containing a power roll as an active ingredient.
[0072] (4- 12) 上記 (4 10)または (4 11)に記載のコラーゲン産生促進剤を有効成 分として含有する外用剤。  [0072] (4- 12) An external preparation containing the collagen production promoter according to (4 10) or (4 11) as an active ingredient.
[0073] (4- 13) 上記 (4 10)または (4 11)に記載のコラーゲン産生促進剤を有効成 分として含有する化粧品。 [0073] (4- 13) A cosmetic comprising the collagen production promoter according to (4 10) or (4 11) as an active ingredient.
[0074] (4- 14) 上記 (4 10)または (4 11)に記載のコラーゲン産生促進剤を有効成 分として含有する医薬部外品。 [0074] (4-14) A quasi-drug containing the collagen production promoter according to (4 10) or (4 11) as an active ingredient.
[0075] (4- 15) 上記 (4 10)または (4 11)に記載のコラーゲン産生促進剤を有効成 分として含有する医薬品。 [0075] (4- 15) The collagen production promoter according to (4 10) or (4 11) above is effectively produced. Pharmaceuticals contained as a fraction.
[0076] (4-16) 上記 (4 10)または (4 11)に記載のコラーゲン産生促進剤を有効成 分として含有する飲食品。 [0076] (4-16) A food and drink containing the collagen production promoter according to (4 10) or (4 11) as an active ingredient.
[0077] さらに、第 1の発明、第 2の発明、第 4の発明を言い換えると、以下のような構成からな [0077] Further, in other words, the first invention, the second invention, and the fourth invention have the following configurations.
[0078] (5-1) 力力ロールを含有することを特徴とする活性酸素消去剤。 [0078] (5-1) An active oxygen scavenger comprising a power roll.
[0079] (5-2) 力力ロールを有効成分として含有し、 [0079] (5-2) Contains a power roll as an active ingredient,
スーパーォキシド、過酸化水素、および一重項酸素からなる群から選択される少なく とも 1種以上の活性酸素を消去することを特徴とする（5— 1)の活性酸素消去剤。  The active oxygen scavenger according to (5-1), wherein at least one active oxygen selected from the group consisting of superoxide, hydrogen peroxide, and singlet oxygen is scavenged.
[0080] (5-3) (5— 1)または（5— 2)に記載の力カロールカ Cacalia属植物力、ら抽出され た力力ロールであることを特徴とする活性酸素消去剤。 [0080] (5-3) A reactive oxygen scavenger characterized by being a force roll extracted from the power of the plant of the genus Cacalia belonging to the genus Calalia, (5-1) or (5-2).
[0081] (5-4) (5— 3)に記載の Cacalia属植物力 モミジガサであることを特徴とする活性 酸素消去剤。 [0081] (5-4) An active oxygen scavenger characterized by being the Cacalia genus plant power Momijigasa described in (5-3).
[0082] (5-5) (5— 1)または（5— 2)に記載の力カロールカ S、化学合成により製造された 力力ロールであることを特徴とする活性酸素消去剤。  [0082] (5-5) The active oxygen scavenger, which is the force Karolka S described in (5-1) or (5-2), a force roll produced by chemical synthesis.
[0083] (5-6) (5—；!）〜（5— 5)のいずれかに記載の活性酸素消去剤を用いて、  [0083] (5-6) Using the active oxygen scavenger according to any one of (5— ;!) to (5-5),
スーパーォキシド、過酸化水素、および一重項酸素からなる群から選択される少なく とも 1種以上の活性酸素を消去することを特徴とする活性酸素消去方法。  A reactive oxygen scavenging method comprising scavenging at least one active oxygen selected from the group consisting of superoxide, hydrogen peroxide, and singlet oxygen.
[0084] (5-7)力力ロールを含有することを特徴とする賦活化剤。  [0084] (5-7) An activator comprising a power roll.
[0085] (5-8) (5— 7)に記載の力カロールカ Cacalia属植物から抽出された力力ロール であることを特徴とする賦活化剤。  [0085] (5-8) An activator characterized in that it is a power roll extracted from the plant of the genus Cacalia belonging to (5-7).
[0086] (5-9) (5— 8)に記載の Cacalia属植物力 モミジガサであることを特徴とする賦活 化剤。 [0086] (5-9) An activator characterized in that it is a Cacalia genus plant power as described in (5-8).
[0087] (5-10) (5— 7)に記載の力カロールカ 化学合成により製造された力力ロールで あることを特徴とする賦活化剤。  [0087] (5-10) An activating agent, which is a force roll produced by chemical synthesis of force kalorka described in (5-7).
[0088] (5-11) [0088] (5-11)
Cacalia属植物の抽出物を有効成分として含有することを特徴とする賦活化剤。  An activator comprising an extract of a genus Cacalia as an active ingredient.
[0089] (5-12) 上記 Cacalia属植物の抽出物が力力ロールを含むことを特徴とする（5— 1 1)の賦活化剤。 [0089] (5-12) The extract of the genus Cacalia contains a force roll (5-1) 1) Activator.
[0090] (5 - 13) 上記 Cacalia属植物がモミジガサであることを特徴とする（5— 11)または（5  [0090] (5-13) (5-11) or (5
12)の賦活化剤。  12) Activator.
[0091] (5 - 14) 力力ロールを含有することを特徴とするコラーゲン産生促進剤。  [0091] (5-14) A collagen production promoter characterized by containing a force roll.
[0092] (5 - 15) (5— 14)に記載の力カロールカ Cacalia属植物から抽出された力カロー ルであることを特徴とするコラーゲン産生促進剤。  [0092] (5-15) A collagen production promoter characterized by being a power carol extracted from a plant of the genus Cacalia belonging to the power carolka described in (5-14).
[0093] (5 - 16) (5— 15)に記載の Cacalia属植物力 S、モミジガサであることを特徴とするコ ラーゲン産生促進剤。 [0093] (5-16) A collagen production promoter characterized by Cacalia genus plant power S or Momijigasa described in (5-15).
[0094] (5 - 17) (5— 14)に記載の力カロールカ S、化学合成により製造された力力ロールで あることを特徴とするコラーゲン産生促進剤。  [0094] (5-17) A collagen production promoter characterized by being the force Karolka S described in (5-14), a force roll produced by chemical synthesis.
[0095] (5 - 18) (5— 1)の活性酸素消去剤、（5— 7)の賦活化剤、（5— 11)の賦活化剤、 および（5— 14)のコラーゲン産生促進剤からなる群から選択される少なくとも 1種以 上を有効成分として含有することを特徴とする抗老化剤。 [0095] (5-18) (5-1) Active oxygen scavenger, (5-7) activator, (5-11) activator, and (5-14) collagen production promoter An anti-aging agent comprising at least one selected from the group consisting of as active ingredients.
[0096] (5 - 19) (5—；!）〜（5— 5)のいずれかの活性酸素消去剤、（5— 7)〜（5— 13)の いずれかの賦活化剤、（5— 14)〜（5— 17)のいずれかのコラーゲン産生促進剤、ま たは（5— 18)の抗老化剤を有効成分として含有する外用剤。 [0096] (5-19) (5— ;!) to (5-5) active oxygen scavenger, (5-7) to (5-13) activator, (5 — An external preparation containing the collagen production promoter according to any one of 14) to (5-17) or the antiaging agent according to (5-18) as an active ingredient.
[0097] (5 - 20) (5— ;!）〜（5— 5)のいずれかの活性酸素消去剤、（5— 7)〜（5— 13)の いずれかの賦活化剤、（5— 14)〜（5— 17)のいずれかのコラーゲン産生促進剤、ま たは（5— 18)の抗老化剤を有効成分として含有する化粧品類。 [0097] (5-20) (5—;!) To (5-5) any active oxygen scavenger, (5-7) to (5-13) any activator, (5 — Cosmetics containing the collagen production promoter according to any one of 14) to (5-17) or the anti-aging agent according to (5-18) as an active ingredient.
[0098] (5 - 21) (5— ;!）〜（5— 5)のいずれかの活性酸素消去剤、（5— 7)〜（5— 13)の いずれかの賦活化剤、（5— 14)〜（5— 17)のいずれかのコラーゲン産生促進剤、ま たは（5— 18)の抗老化剤を有効成分として含有する医薬部外品類。 [0098] (5-21) (5—;!) To (5-5) active oxygen scavenger, (5-7) to (5-13) activator, (5 — Quasi-drugs containing the collagen production promoter of any one of 14) to (5-17) or the anti-aging agent of (5-18) as an active ingredient.
[0099] (5 - 22) (5—；!）〜（5— 5)のいずれかの活性酸素消去剤、（5— 7)〜（5— 13)の いずれかの賦活化剤、（5— 14)〜（5— 17)のいずれかのコラーゲン産生促進剤、ま たは（5— 18)の抗老化剤を有効成分として含有する飲食品類。 [0099] (5-22) (5— ;!) to (5-5) any active oxygen scavenger, (5-7) to any of (5-13) activator, (5 — Foods and beverages containing the collagen production promoter according to any one of 14) to (5-17) or the anti-aging agent according to (5-18) as an active ingredient.
[0100] (5 - 23) (5—；!）〜（5— 5)のいずれかの活性酸素消去剤、（5— 7)〜（5— 13)の いずれかの賦活化剤、（5— 14)〜（5— 17)のいずれかのコラーゲン産生促進剤、ま たは（5— 18)の抗老化剤を有効成分として含有する医薬品類。 発明の効果 [0100] (5-23) (5— ;!) to (5-5) active oxygen scavenger, (5-7) to (5-13) activator, (5 — Pharmaceuticals containing the collagen production promoter according to any one of 14) to (5-17) or the anti-aging agent according to (5-18) as an active ingredient. The invention's effect
[0101] 本発明の第 1の発明により、スーパーォキシド、過酸化水素、および一重項酸素の 複数の活性酸素種に対して効果を発揮する安定性の高い活性酸素消去剤を提供 すること力 sできる。本発明の第 1の発明の活性酸素消去剤は、他の活性酸素消去剤 がー部の活性酸素種に対してのみ消去能を示すのに対して、これら 3種類の活性酸 素種すべてに対して消去作用を有する。通常、生体内で発生する複数の活性酸素 種を消去使用とする場合には、それぞれを消去できる活性酸素消去剤を組み合わ せて用いる必要がある。本発明の第 1の発明の力力ロールは複数の活性酸素種を消 去できること力、ら、力力ロールのみで有効な効果を得ることが期待できる。該活性酸素 消去剤を人体やその他飲食物、医薬品、化粧品、美白剤、肥料、飼料や皮膚外用 剤等に使用することにより、抗酸化効果、特には製品の保存安定性の向上効果等の 優れた酸化防止効果や、各種疾病予防効果ゃ抗酸化効果等を得ることができる。  [0101] According to the first invention of the present invention, it is possible to provide a highly stable active oxygen scavenger that exerts an effect on a plurality of active oxygen species of superoxide, hydrogen peroxide, and singlet oxygen. s can. In the active oxygen scavenger of the first invention of the present invention, other active oxygen scavengers show scavenging ability only for the active oxygen species in the part, whereas all of these three active oxygen species On the other hand, it has an erasing action. Normally, when a plurality of active oxygen species generated in a living body are used for erasing, it is necessary to use a combination of active oxygen scavengers capable of erasing each. The power roll of the first invention of the present invention can be expected to obtain an effective effect only with the power roll, since it can erase a plurality of active oxygen species. Use of the active oxygen scavenger for the human body and other foods and drinks, pharmaceuticals, cosmetics, whitening agents, fertilizers, feeds, skin external preparations, etc. provides an excellent antioxidant effect, particularly an improvement effect on the storage stability of the product. Antioxidant effect and various disease preventive effects can be obtained.
[0102] 本発明の第 2の発明に係る賦活化剤の有効成分として用いるキク科植物の抽出物 は、皮膚繊維芽細胞に対して顕著な賦活作用を有している。したがって、本発明の 第 2の発明に係る賦活化剤は、抗老化剤として優れた効果を発揮する。特に、コゥモ リソゥ属植物の中で山菜として食されている植物は安全性に優れており、本発明の第 2の発明に係る賦活化剤は長期にわたる使用に十分に耐え得ると!/、う効果を奏する [0102] An extract of the Asteraceae plant used as an active ingredient of the activator according to the second invention of the present invention has a significant activation effect on skin fibroblasts. Therefore, the activator according to the second invention of the present invention exhibits an excellent effect as an anti-aging agent. In particular, plants that are eaten as wild plants among the plants belonging to the genus Morris are excellent in safety, and the activator according to the second invention of the present invention is sufficiently resistant to long-term use! / Have an effect
[0103] 本発明の第 3の発明に係る力力ロールを含む植物抽出物の製造方法を用いれば、 煩雑な操作を行うことなく低コストで、低クロロフィル含量の力力ロールを含む植物抽 出物を製造すること力 Sできる。 [0103] By using the method for producing a plant extract containing a power roll according to the third invention of the present invention, a plant extract containing a power roll having a low chlorophyll content can be produced at low cost without performing a complicated operation. The ability to manufacture things S.
[0104] また、本発明の第 4の発明に係るコラーゲン産生促進剤は，コラーゲン産生を促進 する効果に優れ、かつ、安全な力力ロールを有効成分としている。したがって、長期 にわたる使用に十分に耐え得ると!/、う効果を奏する。 [0104] Further, the collagen production promoter according to the fourth invention of the present invention is excellent in the effect of promoting collagen production and contains a safe power roll as an active ingredient. Therefore, it is effective when it can withstand long-term use!
発明を実施するための最良の形態  BEST MODE FOR CARRYING OUT THE INVENTION
[0105] 本発明の Cacalia属とは、キク科コゥモリソゥ属植物のことを指す。また、 Cacalia属は、[0105] The genus Cacalia of the present invention refers to a plant belonging to the genus Compositae. The genus Cacalia is
Parasenecio属と呼はれることもある。 Sometimes called Parasenecio genus.
[0106] まず、本発明の第 1の発明を実施するための最良の形態について説明する。 [0107] 本発明の第 1の発明において、力力ロールとは、 5，6，7，8-テトラハイド口- 3,4,5-トリメ チルナフト [2，3-b]フラン- 9-オール構造を有する非ポリフエノール系の化合物である。 本発明の力力ロールは式 (I)で示される化合物である。 [0106] First, the best mode for carrying out the first invention of the present invention will be described. [0107] In the first invention of the present invention, the power roll refers to 5,6,7,8-tetrahydride-3,4,5-trimethylnaphtho [2,3-b] furan-9-ol It is a non-polyphenol compound having a structure. The power roll of the present invention is a compound represented by the formula (I).
[0108] [化 1]  [0108] [Chemical 1]
Figure imgf000017_0001
Figure imgf000017_0001
[0109] 本発明の第 1の発明で使用できる式 (I)の力力ロールは、本発明の第 1の発明の目 的に沿うものであれば天然産物およびその誘導体、ならびに化学的合成物のいずれ であってもよい。特に、天然産物およびその誘導体が本発明の目的上都合よく使用 できる。 [0109] The power roll of the formula (I) that can be used in the first invention of the present invention is a natural product and its derivative, and a chemical compound as long as it meets the purpose of the first invention of the present invention. Either of them may be used. In particular, natural products and their derivatives can be conveniently used for the purposes of the present invention.
[0110] 活性酸素としては、スーパーォキシド（·〇一）、ヒドロキシラジカル（ΗΟ· )、ヒドロぺ  [0110] Examples of active oxygen include superoxide (· 〇ichi), hydroxy radical (ΗΟ ·), hydrope
2 ラジカル (LOO ' )、一酸化窒素（NO)などのラジカル種、および過酸化水素（H O )  2 radical species (LOO '), radical species such as nitric oxide (NO), and hydrogen peroxide (H 2 O 2)
2 2 twenty two
、一重項酸素 〇）、ペルォキシナイトライト（ONOO—）、脂質ヒドロペルォキシド (L , Singlet oxygen 〇), peroxynitrite (ONOO—), lipid hydroperoxide (L
2  2
OOH)、次亜塩素酸（HOC1)、オゾン（O )などのノンラジカル種が挙げられる。この  Non-radical species such as OOH), hypochlorous acid (HOC1) and ozone (O 2). this
3  Three
うち、スーパーォキシド（·〇 ―）、ヒドロキシラジカル (HO · )、過酸化水素（H O )お  Of these, superoxide (· ○ ―), hydroxy radical (HO ·), hydrogen peroxide (H 2 O)
2 2 2 よび一重項酸素 〇）は、狭義の活性酸素と呼ばれる。 [0111] これらの活性酸素力 生体に及ぼす影響としては、 DNA螺旋の部分開裂、連鎖的ラ ジカルの発生による組織の損傷などが挙げられる。その結果として、気管支喘息など のアレルギー反応の惹起；ヒスタミン放出による炎症の惹起；心筋梗塞、狭心症など の虚血性心疾患、劇症肝炎、脂肪肝などの肝臓障害などの疾患の悪化;脳梗塞など の脳血管障害、脳組織の破壊などによる痴呆の誘発;未熟児網膜症、肺気腫、糖尿 病、痛風、皮膚癌、妊娠中毒症など疾病の一因となることが知られている。 2 2 2 and singlet oxygen O) are called active oxygen in the narrow sense. [0111] These reactive oxygen forces have effects on the living body, including partial cleavage of DNA helix and tissue damage due to the generation of chain radicals. As a result, allergic reactions such as bronchial asthma are induced; inflammation is induced by histamine release; ischemic heart diseases such as myocardial infarction and angina pectoris; worsening diseases such as hepatic disorders such as fulminant hepatitis and fatty liver; brain Induction of dementia due to cerebrovascular disorders such as infarction and destruction of brain tissue; retinopathy of prematurity, emphysema, diabetes, gout, skin cancer, and pregnancy toxemia are known to contribute to diseases.
[0112] 本発明の第 1の発明の最も重要な開示は、力力ロールに、スーパーォキシド、過酸 化水素、および一重項酸素の複数の活性酸素種に対して高!/、活性酸素消去効果を 見いだし、それにも関わらず高い安定性を有する活性酸素消去剤を提供するに至つ たことである。従来、力力ロールには、 DPPH消去能、過酸化脂質抑制能などを有す ることが知られていた。しかし、 DPPH消去能、過酸化脂質抑制能があるからといつ て、実際に生体内で発生するスーパーォキシド、過酸化水素、一重項酸素等の活性 酸素種に対する消去作用があるとはかぎらない。また、一般に抗酸化剤は安定性が 低ぐ化粧料などに配合した場合の安定性に問題があり、実用性に乏しい。本発明 により、複数の活性酸素種に対し高い活性酸素消去効果があり、かつ安定性に高い 活性酸素消去剤を提供できるようになった。  [0112] The most important disclosure of the first invention of the present invention is that the power roll has high! /, Active oxygen relative to a plurality of reactive oxygen species of superoxide, hydrogen peroxide, and singlet oxygen. The present inventors have found an scavenging effect and nevertheless have provided an active oxygen scavenger having high stability. Conventionally, it has been known that a power roll has DPPH elimination ability, lipid peroxide inhibition ability and the like. However, because of DPPH scavenging ability and lipid peroxide inhibiting ability, it does not necessarily have a scavenging action on active oxygen species such as superoxide, hydrogen peroxide, and singlet oxygen that are actually generated in the living body. . In general, antioxidants have a problem in stability when blended with cosmetics or the like having low stability, and are not practical. According to the present invention, an active oxygen scavenger having a high active oxygen scavenging effect for a plurality of active oxygen species and having high stability can be provided.
[0113] 本発明の第 1の発明の力力ロールを得るための植物は、特に限定されるものではな いが、例えば双子葉植物、単子葉植物、草本性植物、木本性植物、ゥリ科植物、ナ ス科植物、イネ科植物、アブラナ科植物、マメ科植物、ァオイ科植物、キク科植物、ァ カザ科植物、マメ科の植物などが挙げられる。  [0113] The plant for obtaining the power roll of the first invention of the present invention is not particularly limited. For example, dicotyledonous plants, monocotyledons, herbaceous plants, woody plants, uri Plants, NASA plants, Gramineae plants, Brassicaceae plants, Leguminous plants, Cyprinaceae plants, Asteraceae plants, Aceraceae plants, and Leguminous plants.
好ましくは、キク科植物がよい。  Asteraceae is preferable.
[0114] キク科植物としては、キク属（Dendranthema)、シォン属（Aster)、キンセンカ属（Calen dula)、ェゾギク属 (Callistephus)、ベニバナ属 (Carthamus)、コスモス属 (Cosmos)、 ダリァ属（Dahlia)、ガザニァ属（Gazania)、ガーベラ属（Gerbera)、ヒマヮリ属（Helianth us)、セネシォ属（Senecio)、ジニァ属（Zinnia)、ノコギリソゥ属 (Achillea)、アグラタム 属 Ageratum)、カイザイク属 (Ammobium)、ヒナギク属 (Bellis)、ャグノレマソゥ属（Centa urea)、ァザミ属 (Cirsium)、コレオプシス属 (Coreopsis)、ディモノレフォセォ属 (Dimorp hotheca)、ェキノプス属（Echinops)、フェリシア属 (Felicia)、ガイラルディア属（Gaillar dia)、ミヤコヮスレ属 (Gymnaster)、ムギワラギク属（Helichrysum)、ローダンセ属 (Heli pterum)、リアトリス属 (Liatris)、ルドべキア属（Rudbeckia)、ストケシァ属（Stokesia)、 タゲテス属（Tagetes)、コゥモリソゥ属 (Cacalia)、ォォモミジガサ属（Miricacalia)、ャブ レガサ属（Syneilesis)などが挙げられる力 その中でも、コゥモリソゥ属力 S望ましい。 [0114] The Asteraceae plants include the genus Dendranthema, Aster, Calend dula, Callistephus, Carthamus, Cosmos, and Dahlia. ), Gazania, Gerbera, Helianth us, Senecio, Zinnia, Achillea, Ageratum, Ammobium, Daisies (Bellis), Centa urea, Cirsium, Coreopsis, Dimorp hotheca, Echinops, Felicia, Guilaldia (Gaillar dia), Miyako genus (Gymnaster), wheat genus (Helichrysum), Rhodanse genus (Heli pterum), riatris (Liatris), Rudbeckia, Stokesia, Tagetes, bats Powers such as (Cacalia), Miricacalia, and Syneilesis. Among them, the bat power S is desirable.
[0115] コゥモリソゥ属植物としては、コゥモリソゥ、モミジガサ、力ニコゥモリ、タマブキ、ィヌド ゥナ、ィズカニコゥモリ、ォォカニコゥモリ、タイミンガサ、ックシコゥモリソゥ、テバコモミ ジガサ、ニシノヤマタイミンガサ、ミミコゥモリ、モミジコゥモリ、ヤマタィミンガサ、ョブス マソゥなどが挙げられる。その中でも、食用植物が好ましぐモミジガサが適している。 本発明の第 1の発明においてモミジガサとは、別名、シドケ、シドキ、シドキナ、シン ドキ、キノシタ、トウキチ、トウキチナ、スドケ、シトギ、スドケと呼ばれており、キク科の 植物で国内に広く分布し山菜として食されている。  [0115] The plants of the genus Komorisou include bat Morisou, Momijigasa, Nikkomori, Tamabuki, Innuduna, Izkanikomori, Okanikomori, Taimingasa, Kushikomorisou, Tebacomomi Jigasa, Nishinoyama Taimingasa, Mikamimori Etc. Among them, Momijigasa, which is preferred by edible plants, is suitable. In the first invention of the present invention, Momijigasa is also known as Sidke, Sidoki, Sidkina, Shindoki, Kinoshita, Toukichi, Tokichina, Sudoke, Sitgi, Sudoke, and is widely distributed in the family Asteraceae. It is eaten as a wild vegetable.
[0116] 力力ロールを得るための植物組織としては、特に限定はされないが、植物全体、あ るいは部分的な組織から得ることができる。得ること力 Sできる部位としては、特に限定 されないが全樹、花、蕾、子房、果実、葉、子葉、茎、芽、根、種子、乾燥種子、胚、 胚芽、根などである。本発明の第 1の発明では、好ましくは葉、茎、根から抽出操作を fiうのがよい。  [0116] The plant tissue for obtaining the power roll is not particularly limited, but can be obtained from the whole plant or a partial tissue. Sites that can be obtained are not particularly limited, but include whole trees, flowers, buds, ovary, fruits, leaves, cotyledons, stems, buds, roots, seeds, dried seeds, embryos, germs, roots and the like. In the first invention of the present invention, it is preferable to perform the extraction operation from leaves, stems and roots.
[0117] 力力ロールを植物から得るための方法としては、植物を生の状態、凍結された状態 、凍結乾燥された状態のいずれから抽出操作を行ってもよい。好ましくは、凍結乾燥 の状態から抽出作業を行うのがよい。生または凍結された植物から抽出作業を行う場 合には、まず、破砕をしても良い。破砕にはミキサー、ホモジナイザー、乳鉢などを用 いて破砕すればよい。凍結乾燥されたものは、凍結乾燥操作後、粉状に破砕しても 良い。  [0117] As a method for obtaining a power roll from a plant, the plant may be extracted from a raw state, a frozen state, or a lyophilized state. The extraction operation is preferably performed from a freeze-dried state. When extracting from raw or frozen plants, it may be crushed first. For crushing, a mixer, a homogenizer, a mortar or the like may be used. The freeze-dried product may be crushed into powder after the freeze-drying operation.
[0118] 破砕された植物体は溶媒を用いて、抽出操作を行う。抽出方法として、室温，冷却又 は加熱した状態で含浸させて抽出する方法、水蒸気蒸留などの蒸留法を用いて抽 出する方法、圧搾して抽出物を得る圧搾法、超臨界流体又は亜臨界流体を用いて 抽出する方法などを用いてもよぐこれらの方法を単独で、又は 2種以上を組み合わ せて抽出を行っても良い。好ましくは、細かく裁断した原料 lgに対して、 5〜100ml の溶媒を用い、 1時間から 1か月間、好ましくは 1〜5日間、室温或いは加熱下で行う ことが望ましい。 [0118] The crushed plant body is extracted using a solvent. Extraction methods include extraction by impregnation at room temperature, in a cooled or heated state, extraction using a distillation method such as steam distillation, compression method to obtain an extract by pressing, supercritical fluid or subcritical fluid These extraction methods using fluid may be used alone or in combination of two or more. Preferably, 5 to 100 ml of solvent is used for the finely cut raw material lg for 1 hour to 1 month, preferably 1 to 5 days, at room temperature or under heating. It is desirable.
[0119] 抽出に用いられる溶媒は、有効成分が効果的に抽出される溶媒であれば特に限定 されるものではないが、水または、エタノール等の低級アルコールのように水と混和 する有機溶媒、またはそれらの混液、または水と混和しない有機溶媒たとえば酢酸ェ チル等を用いてもよい。そのほかにも例を挙げるならば、ジクロロメタン、メタノーノレ， イソプロパノール，イソブタノール， n-へキサノーノレ，メチノレアミノレアノレコーノレ， 2-ェチ ノレブタノール， n-ォクチルアルコール等の 1価アルコール類、グリセリン，エチレングリ コーノレ，エチレングリコーノレモノメチノレエーテノレ，プロピレングリコーノレ，プロピレング リコーノレモノメチノレエーテノレ，プロピレングリコーノレモノェチノレエーテノレ， トリエチレン グリコール， 1,3-ブチレングリコール， へキシレングリコール等の多価アルコール又は その誘導体、アセトン，メチルェチルケトン，メチルイソブチルケトン，メチル -n-プロピ ルケトン等のケトン類，酢酸イソプロピル等のエステル類、ェチルエーテル，イソプロ ピルエーテル， n-ブチルエーテル等のエーテル類、スクヮラン，ワセリン，パラフィンヮ ッタス，ノ ラフィン油などの炭化水素類、ォリーブ油，小麦胚芽油，米油，ゴマ油，マ 力ダミアンナッツ油，アルモンド油，ヤシ油等の植物油脂、牛脂，豚脂，鯨油等の動 物油脂などが例示される。また、リン酸緩衝生理食塩水等の無機塩類を添加した極 性溶媒、界面活性剤を添加した溶媒を用いることもでき、特に限定されない。飲食物 等への使用、皮膚への塗布を含む人体への使用における安全性等を考えれば、特 に、水および/またはアルコールのいずれかにより抽出することが好ましい。  [0119] The solvent used for the extraction is not particularly limited as long as the active ingredient is effectively extracted, but is water or an organic solvent miscible with water such as a lower alcohol such as ethanol, Alternatively, a mixed solution thereof, or an organic solvent immiscible with water, such as ethyl acetate, may be used. Other examples include monohydric alcohols such as dichloromethane, methanolol, isopropanol, isobutanol, n-hexanol, methinoreaminoreanolol, 2-ethynolebutanol, n-octyl alcohol, Glycerin, ethylene glycolate, ethylene glycol monomethino ethenore, propylene glycol nore, propylene glycol mono methino ethenore, propylene glycol nole mono chineno ethenore, triethylene glycol, 1,3-butylene glycol, Polyhydric alcohols such as hexylene glycol or derivatives thereof, ketones such as acetone, methyl ethyl ketone, methyl isobutyl ketone, methyl-n-propyl ketone, esters such as isopropyl acetate, ethyl ether, isopropyl ether , Ethers such as n-butyl ether, hydrocarbons such as squalene, petrolatum, paraffin tussus, norafin oil, olive oil, wheat germ oil, rice oil, sesame oil, mussian damian nut oil, almond oil, coconut oil, etc. Examples include animal fats such as vegetable oils, beef tallow, pork tallow and whale oil. In addition, a polar solvent to which inorganic salts such as phosphate buffered saline are added and a solvent to which a surfactant is added can be used, and there is no particular limitation. In consideration of safety in use to foods and drinks, use on the human body including application to the skin, etc., extraction with either water and / or alcohol is particularly preferable.
[0120] また、段階的に抽出操作を行っても良い。たとえば、エタノール等の低級アルコー ルのように水と混和する有機溶媒で抽出操作を行い、エバポレーターなどで濃縮し、 水と混和しない有機溶媒たとえば酢酸ェチル等と水を加え、有機層と水層に分離し ても良い。この場合、有機層にも水層にもチロシナーゼ阻害作用、メラニン生成抑制 作用、抗酸化作用がある成分が抽出されるので、用途別に利用しても構わない。例 えば、水層の成分は、洗顔用途、化粧水用途及び入用剤用途など水と親和性が必 要な抗酸化剤が所望される場合に利用が期待でき、また、有機層のものはクリーム及 び乳液など油と親和性が必要な抗酸化剤が所望される場合に利用が期待できるが、 これらに限定されるものではない。 [0121] このようにして得られた力力ロールは、そのまま用いることもできる力 その効果を失わ ない範囲で、脱臭や脱色などの精製操作を加えたりして用いてもよい。また、抽出物 中の有効成分である力力ロールの濃度を高めるために、所望により、得られた抽出物 を更に、濃縮、液液分配、吸着クロマトグラフィー、順相もしくは逆相クロマトグラフィ 一等の手段に付すことも可能である。 [0120] The extraction operation may be performed in stages. For example, extraction is performed with an organic solvent that is miscible with water, such as a lower alcohol such as ethanol, and the mixture is concentrated with an evaporator. May be separated. In this case, components having a tyrosinase inhibitory action, a melanin production inhibitory action, and an antioxidant action are extracted in both the organic layer and the aqueous layer, and may be used for different purposes. For example, the components of the water layer can be expected to be used when an anti-oxidant that has an affinity for water is desired, such as facial cleansing applications, skin lotion applications, and use applications. Although it can be expected to be used when an antioxidant that requires an affinity for oil such as cream and milky milk is desired, it is not limited to these. [0121] The force roll obtained in this way may be used as it is, as long as it does not lose its effect. In addition, in order to increase the concentration of the force roll, which is an active ingredient in the extract, the obtained extract can be further concentrated, liquid-liquid distribution, adsorption chromatography, normal phase or reverse phase chromatography, etc., if desired. It is also possible to attach to the means.
[0122] 力力ロールを得る植物としては植物加工物であってもよい。その加工方法は、植物 を水、有機溶媒、水と有機溶媒の混合物などを用いて、低温、室温、加温条件下で の含浸法、蒸留法、圧搾法、超音波法、超臨界流体法、亜臨界流体法などで抽出物 を回収する。さらに植物や植物から回収した抽出物を発酵させるなどの加工処理した 加工物なども含まれる。例えば植物抽出物や植物エキスなどが挙げられる。  [0122] The plant from which a powerful roll is obtained may be a processed plant product. The processing methods include impregnation method, distillation method, squeezing method, ultrasonic method, supercritical fluid method using water, organic solvent, water and organic solvent mixture at low temperature, room temperature and warm conditions. Extract the extract using the subcritical fluid method. Furthermore, it includes processed products such as fermenting plants and extracts collected from plants. Examples include plant extracts and plant extracts.
[0123] 本発明の第 1の発明の力力ロールを得るための手段として、化学合成が挙げられる。  [0123] As a means for obtaining the power roll of the first invention of the present invention, chemical synthesis can be mentioned.
力力ロールの合成に関しては、 Chemical.  For the synthesis of force rolls, see Chemical.
Letters., 12, 1317(1975)、 Journal of chemical research., 2, 109(2007)、 Heterocycles  Letters., 12, 1317 (1975), Journal of chemical research., 2, 109 (2007), Heterocycles
65， 319(2005)などの方法が例示することができる。このように化学合成により得られる 力力ロールも本発明に係る活性酸素消去剤の有効成分として好適に用いることがで きる。 A method such as 65, 319 (2005) can be exemplified. Thus, a power roll obtained by chemical synthesis can also be suitably used as an active ingredient of the active oxygen scavenger according to the present invention.
[0124] 本発明の第 1の発明に係る力力ロールの使用形態は任意であり、例えば、粗抽出物 のまま、有効成分である力力ロールの含量を高めた精製品、あるいは有効成分である 力力ロール純品として使用することができる。  [0124] The use form of the power roll according to the first invention of the present invention is arbitrary, for example, a refined product with an increased content of the power roll, which is an active ingredient, or an active ingredient as a crude extract. Some power rolls can be used as pure products.
[0125] 本発明の第 1の発明に係る力力ロールを活性酸素消去剤として、化粧料、飲食品、 医薬品等に添加する際の添加量については、当該化合物によって期待される効果 が有効に発揮される量であれば良ぐ特に制限はない。例えば、化粧料や飲食品に 添カロする場合は、全体量の 0. 00000001〜； 1 %、好まし <は 0. 000001—0. 01 % 程度が適当である。  [0125] With regard to the addition amount when the power roll according to the first invention of the present invention is added as an active oxygen scavenger to cosmetics, foods and drinks, pharmaceuticals, etc., the effect expected by the compound is effective. There is no particular limitation as long as it is an amount to be exhibited. For example, in the case of supplementing with cosmetics and foods and beverages, it is appropriate that the total amount is from 0.0000000001 to 1%, preferably <0.0001% to 0.01%.
[0126] 本発明の第 1の発明における活性酸素消去剤は、本発明の必須の力力ロールの他 に、下記に示すような化粧品類などで通常使用される基剤、添加剤など、例えば油 脂類、ロウ類、鉱物油、脂肪酸類、アルコール類、エステル類、金属セッケン、ガム質 および水溶性高分子化合物、界面活性剤、ビタミン類、アミノ酸、美白剤、保湿剤、 微生物培養代謝産物、 ヒドロキシ酢酸、無機顔料、紫外線吸収剤、収斂剤、抗菌 剤、殺菌，消毒薬、頭髪用剤、香料、色素 ·着色剤、甘味料、栄養強化剤、育毛剤、 動植物抽出物、抗酸化剤、抗炎症剤等と併用して用いることができる。 [0126] The active oxygen scavenger in the first invention of the present invention includes, in addition to the essential power roll of the present invention, bases and additives that are usually used in cosmetics as shown below, for example, Oils and fats, waxes, mineral oils, fatty acids, alcohols, esters, metal soap, gum And water-soluble polymer compounds, surfactants, vitamins, amino acids, whitening agents, moisturizers, microbial culture metabolites, hydroxyacetic acid, inorganic pigments, UV absorbers, astringents, antibacterial agents, bactericides, disinfectants, hair It can be used in combination with agents, fragrances, pigments / colorants, sweeteners, nutrition enhancers, hair restorers, animal and plant extracts, antioxidants, anti-inflammatory agents and the like.
[0127] 油脂類としては、アポガド油、アルモンド油、ウイキヨゥ油、エゴマ油、ォリーブ油、ォ レンジ油、オレンジラファー油、ゴマ油、カカ才 β旨、力ミツレ油、力ロット油、キューカン バー油、牛脂、牛脂脂肪酸、ククイナッツ油、サフラワー油、大豆油、ツバキ油、トウモ ロコシ油、ナタネ油、パーシック油、ヒマシ油、綿実油、落花生油、タートル油，ミンク 油、卵黄油、カカオ脂、パーム油、パーム核油、モクロウ、ヤシ油、牛脂、豚脂、硬化 油、硬化ヒマシ油などが挙げられる。  [0127] The fats and oils include apogad oil, almond oil, wikiyo oil, sesame oil, olive oil, orange oil, orange rafa oil, sesame oil, kaka-age β, power mitsule oil, power lot oil, cucumber oil, Beef tallow, beef tallow fatty acid, cucumber nut oil, safflower oil, soybean oil, camellia oil, corn oil, rapeseed oil, persic oil, castor oil, cottonseed oil, peanut oil, turtle oil, mink oil, egg yolk oil, cocoa butter, palm oil , Palm kernel oil, owl, coconut oil, beef tallow, lard, hydrogenated oil, hydrogenated castor oil.
[0128] 例えば、ロウ類としては、ミツロウ、カルナバロウ、鯨ロウ、ラノリン、液状ラノリン、還 元ラノリン、硬質ラノリン、カンデリラロゥ、モンタンロウ、セラックロウなどが挙げられる。  [0128] Examples of waxes include beeswax, carnauba wax, whale wax, lanolin, liquid lanolin, reduced lanolin, hard lanolin, candelillaro, montan wax, shellac wax and the like.
[0129] 例えば、鉱物油としては、流動パラフィン、ワセリン、パラフィン、ォゾケライド、セレシ ン、マイクロクリスタンワックス、ポリエチレン末、スクワレン、スクヮラン、プリスタンなど が挙げられる。  [0129] Examples of mineral oils include liquid paraffin, petrolatum, paraffin, ozokelide, ceresin, microcrystalline wax, polyethylene powder, squalene, squalane, and pristane.
[0130] 脂肪酸類としては、ラウリン酸、ミリスチン酸、ノルミチン酸、ステアリン酸、ベヘン酸、 ォレイン酸、 12-ヒドロキシステアリン酸、ゥンデシレン酸、トール油、ラノリン脂肪酸な どの天然脂肪酸、イソノナン酸、カプロン酸、 2—ェチルブタン酸、イソペンタン酸、 2 ーメチルペンタン酸、 2—ェチルへキサン酸、イソペンタン酸などの合成脂肪酸が挙 げられる。  [0130] Examples of fatty acids include lauric acid, myristic acid, normitic acid, stearic acid, behenic acid, oleic acid, 12-hydroxystearic acid, undecylenic acid, tall oil, lanolin fatty acid, isononanoic acid, caproic acid And synthetic fatty acids such as 2-ethylbutanoic acid, isopentanoic acid, 2-methylpentanoic acid, 2-ethylhexanoic acid and isopentanoic acid.
[0131] アルコール類としては、エタノール、イソプロパノール、ラウリノレアノレコーノレ、セタノー ノレ、 ステアリノレアノレコーノレ、ォレイノレアノレコーノレ、ラノリンァノレコーノレ、コレステロ一ノレ 、フィトステロールなどの天然アルコーノレ、 2—へキシルデカノーノレ、イソステアリルァ ノレコーノレ、 2—オタチルドデカノールなどの合成アルコール、酸化エチレン、エチレン グリコーノレ、ジエチレングリコーノレ、トリエチレングリコーノレ、エチレングリコーノレモノエ チルエーテル、エチレングリコールモノブチルエーテル、ジエチレングリコールモノメ チノレエーテノレ、ジエチレングリコーノレモノェチノレエーテノレ、ポリエチレングリコーノレ、 酸化プロピレン、プロピレングリコーノレ、ポリプロピレングリコール、 1 , 3—ブチレングリ コーノレ、グリセリン、ノ チノレアノレコーノレ、ペンタエリトリトーノレ、ソノレビトーノレ、 マンニトー ノレ、ブドウ糖、ショ糖などの多価アルコール類などが挙げられる。 [0131] Examples of alcohols include natural alcohols such as ethanol, isopropanol, laurino-leanolone, cetanol, stearino-leno-conole, oleino-leano-reconole, lanolinano-reconole, cholesterol, and phytosterol. Synthetic alcohols such as 2-hexyl decanol, isostearyl decanol, 2-octyldodecanol, ethylene oxide, ethylene glycol, diethylene glycol, triethylene glycol, ethylene glycol monoethyl ether, ethylene glycol monobutyl ether , Diethylene glycol monomethylenoate, diethylene glycol monoethylenoate, polyethylene glycol, propylene oxide, propylene glycol, polypro Pyrene glycol, 1,3-butylenegri Examples include cornore, glycerin, chinenoreanoleconore, pentaerythritol, sonorebitonore, mannitol, glucose, sucrose and other polyhydric alcohols.
[0132] エステル類としては、ミリスチン酸イソプロピル、パルミチン酸イソプロピル、ステアリン 酸ブチル、ラウリン酸へキシル、ミリスチン酸ミリスチル、ォレイン酸ォレイル、ォレイン 酸デシル、ミリスチン酸オタチルドデシル、ジメチルオクタン酸へキシルデシル、乳酸 セチル、乳酸ミリスチル、フタル酸ジェチル、フタル酸ジブチル、酢酸ラノリン、モノス テアリン酸エチレングリコール、モノステアリン酸プロピレングリコール、ジォレイン酸 プロピレングリコールなどが挙げられる。  [0132] Esters include isopropyl myristate, isopropyl palmitate, butyl stearate, hexyl laurate, myristyl myristate, oleyl oleate, decyl oleate, octyldodecyl myristate, hexyl decyl dimethyloctanoate, lactic acid Examples include cetyl, myristyl lactate, jetyl phthalate, dibutyl phthalate, lanolin acetate, ethylene glycol monostearate, propylene glycol monostearate, and propylene glycol dioleate.
[0133] 例えば、金属セッケンとしては、ステアリン酸アルミニウム、ステアリン酸マグネシウム 、ステアリン酸亜鉛、ステアリン酸カルシウム、パルミチン酸亜鉛、ミリスチン酸マグネ シゥム、ラウリン酸亜鉛、ゥンデシレン酸亜鉛などが挙げられる。  [0133] Examples of the metal soap include aluminum stearate, magnesium stearate, zinc stearate, calcium stearate, zinc palmitate, magnesium myristate, zinc laurate, and zinc undecylenate.
[0134] 例えば、ガム質および水溶性高分子化合物としては、アラビアゴム、ベンゾインゴム 、ダンマルゴム、グアヤタ脂、アイルランド苔、カラャゴム、トラガントゴム、キヤロブゴム 、クインシード、寒天、カゼイン、デキストリン、ゼラチン、ぺクチン、デンプン、カラギー ン、キトサン塩、硫酸化キチン、リン酸化キチン、アルギン酸およびその塩、ヒアルロン 酸およびその塩、コンドロイチン硫酸、へパリン、ェチルセルロース、メチルセルロー  [0134] For example, gums and water-soluble polymer compounds include gum arabic, benzoin rubber, dammar gum, guayata fat, Irish moss, kala gum, tragacanth gum, kilob gum, quinseed, agar, casein, dextrin, gelatin, pectin, Starch, carrageen, chitosan salt, sulfated chitin, phosphorylated chitin, alginic acid and its salt, hyaluronic acid and its salt, chondroitin sulfate, heparin, ethyl cellulose, methylcellulose
ロセノレロース、結晶セノレロース、ポリビニノレアノレコーノレ、ポリビニノレメチノレエーテノレ、 ポリビュルピロリドン、ポリビュルメタアタリレート、ポリアクリル酸塩、ポリエチレンォキ サイドやポリプロピレンオキサイド等のポリアルキレンオキサイドまたはその架橋重合 物、カルボキシビュルポリマー、ポリエチレンィミンなどが挙げられる。 Rosenorelose, crystalline senorelose, polyvinylenorenoleconole, polyvinino methinoreatenore, polybulol pyrrolidone, polybull metaaterylate, polyacrylic acid salt, polyalkylene oxide such as polyethylene oxide and polypropylene oxide or cross-linking thereof Polymers, carboxybule polymers, polyethyleneimine and the like can be mentioned.
[0135] 界面活性剤としては、ァニオン界面活性剤（カルボン酸塩，スルホン酸塩，硫酸エス テル塩，リン酸エステル塩）、カチオン界面活性剤（ァミン塩， 四級アンモニゥム塩）、 両性界面活性剤（カルボン酸型両性界面活性剤，硫酸エステル型両性界面活性剤 ,スルホン酸型両性界面活性剤，リン酸エステル型両性界面活性剤）、非イオン界面 活性剤（エーテル型非イオン界面活性剤，エーテルエステル型非イオン界面活性剤 , エステル型非イオン界面活性剤，ブロックポリマー型非イオン界面活性剤，含窒素 型非イオン界面活性剤）、その他の界面活性剤 (天然界面活性剤，タンパク質加水 分解物の誘導体，高分子界面活性剤，チタン'ケィ素を含む界面活性剤，フッ化炭 素系界面活性剤などが挙げられる。 [0135] Surfactants include anionic surfactants (carboxylates, sulfonates, sulfate esters, phosphate esters), cationic surfactants (ammine salts, quaternary ammonium salts), amphoteric surfactants. Agent (carboxylic acid type amphoteric surfactant, sulfate ester type amphoteric surfactant, sulfonic acid type amphoteric surfactant, phosphate ester type amphoteric surfactant), nonionic surfactant (ether type nonionic surfactant, Ether ester type nonionic surfactant , Ester type nonionic surfactant, block polymer type nonionic surfactant, nitrogen-containing type nonionic surfactant), other surfactants (natural surfactants, derivatives of protein hydrolysates, polymer surfactants) Agents, surfactants containing titanium 'key, fluorocarbon surfactants, and the like.
[0136] ビタミン類としては、ビタミン Α群ではレチノール、レチナール（ビタミン A1)、デヒドロレ チナール（ビタミン A2)、カロチン、リコピン（プロビタミン A)、ビタミン B群では、チアミ ン塩酸塩、チアミン硫酸塩（ビタミン B1)、リボフラビン（ビタミン B2)、ピリドキシン（ビタ ミン B6)、シァノコバラミン（ビタミン B12)、葉酸類、ニコチン酸類、パントテン酸類、ビ ォチン類、コリン、イノシトール類、ビタミン C群では、ァスコルビン酸及びその誘導体 、ビタミン D群では、ェルゴカルシフエロール（ビタミン D2)、コレカルシフエロール（ビ タミン D3)、ジヒドロタキステロール、ビタミン E群では、トコフエロール及びその誘導体 、ュビキノン類、ビタミン K群では、フイトナジオン（ビタミン K1)、メナキノン（ビタミン K2 )、メナジオン（ビタミン K3)、メナジオール（ビタミン K4)などが挙げられる。  [0136] Vitamins include retinol, retinal (vitamin A1), dehydroretinal (vitamin A2), carotene, lycopene (provitamin A), and thiamin hydrochloride and thiamine sulfate (vitamin B group). Vitamin B1), riboflavin (vitamin B2), pyridoxine (vitamin B6), cyanocobalamin (vitamin B12), folic acid, nicotinic acids, pantothenic acids, biotins, choline, inositols, ascorbic acid in the vitamin C group And its derivatives, in vitamin D group, ergocalciferol (vitamin D2), cholecalciferol (vitamin D3), dihydrotachysterol, in vitamin E group, tocopherol and its derivatives, ubiquinones, in vitamin K group , Huitnadione (vitamin K1), Menaquinone (vitamin K2), Me Dione (vitamin K3), and the like menadiol (vitamin K4).
[0137] アミノ酸としては、ノ リン、ロイシン、イソロイシン、トレオニン、メチォニン、フエ二ルァラ ニン、トリプトファン、リジン、グリシン、ァラニン、ァスパラギン、グルタミン、セリン、シス ティン、シスチン、チロシン、プロリン、ヒドロキシプロリン、ァスパラギン酸、グルタミン 酸、ヒドロキシリジン、アルギニン、オル二チン、ヒスチジンなどや、それらの硫酸塩、リ ン酸塩、硝酸塩、クェン酸塩、あるいはピロリドンカルボン酸の如きアミノ酸誘導体な どが挙げられる。  [0137] Amino acids include Norin, leucine, isoleucine, threonine, methionine, phenylalanine, tryptophan, lysine, glycine, alanine, asparagine, glutamine, serine, cystine, cystine, tyrosine, proline, hydroxyproline, asparagine Examples include acids, glutamic acid, hydroxylysine, arginine, ornithine, histidine, and their amino acid derivatives such as sulfate, phosphate, nitrate, citrate, and pyrrolidone carboxylic acid.
[0138] 美白剤として、 Lーァスコルビン酸及びその誘導体、ハイドロキノン及びその誘導体、 トラネキサム酸、ルシノール、ェデト酸及びその誘導体、並びに胎盤抽出物、 t-AMC HA、ァセロラエキス、エイジッエキス、エラグ酸またはその誘導体、火辣エキス、カミ ッレエキス，力ミツレ花エキス ·（尿素）、キウイエキス、グルタチオン、トコトリェノール、 フェルラ酸、ラズベリーケトン、ノレシノール、ゥワウルシェキス、ジパルミチン酸ピリドキ シン、ィォゥ、コウジ酸またはその誘導体、ダルコサミンまたはその誘導体、ヒドロキシ ケィヒ酸またはその誘導体、ダルタチオン、アル二力エキス、ォゥゴンエキス、ソゥハク ヒエキス、サイコエキス、ボウフゥエキス、マンネンタケ菌糸体培養物またはその抽出 物、シナノキエキス、モモ葉エキス、エイジッエキス、クジンエキス、ジュエキス、トウキ エキス、ョクイユンエキス、カキ葉エキス、ダイ才ゥエキス、ボタンピエキス、ノヽマメリス エキス、マロニエエキス、オトギリソゥエキス、油溶性カンゾゥエキスなどが挙げられる 保湿剤として、ピリドンカルボン酸ナトリウム、グリコール、グリセリン、グルコース、マル トース、マルチトーノレ、ショ糖、フラクトース、キシリトーノレ、ソノレビトーノレ、マノレトトリオ一 ス、スレイトール、エリスリトーノレ、デンプン分解糖還元アルコール、ソノレビトーノレ、多 価アルコール類としては、エチレングリコール、 1 , 4ーブチレングリコール、ジグリセリ ン、トリグリセリン、テトラグリセリン、 1 , 3—ブチレングリコール、プロピレングリコール、 ジプロピレングリコール、ポリエチレングリコール、 1 , 3—プロパンジオール、その他セ リン、グリシン、スレオニン、ァラニン、コラーゲン、加水分解コラーゲン、ヒドロネクチン 、フイブロネクチン、ケラチン、エラスチン、ローヤルゼリー、コンドロイチン硫酸へパリ ン、グリセ口リン脂質、グリセ口糖脂質、スフインゴリン脂質、スフインゴ糖脂質、リノール 酸またはそのエステル類、エイコサペンタエン酸またはそのエステル類、ぺクチン、ビ フィズス菌発酵物、乳酸発酵物、酵母抽出物、レイシ菌糸体培養物またはその抽出 物、小麦胚芽油、アポガド油、米胚芽油、ホホバ油、ダイズリン脂質、 Ί オリザノー ノレ、ビロウドアオイエキス、ョタイニンエキス、ジォゥエキス、タイソゥエキス、カイソゥェ キス、キダチアロエエキス、ゴボウエキス、マンネンロウエキス、ァノレニ力エキス、 /j、麦 フスマ、ピロリドンカルボン酸、トマトエキス、ツバキ油、大豆リン脂質、ヒアルロン酸、ト レオニン、グリコール酸アンモニゥム、アルギン酸メチルシラノール、ョタイニン、トウキ エキス、トウキ根エキス、ダイス、エキス、アスパラガスエキス、 DNA— Na、 PCA-Na, R NA-Na,ァシタバエキス、ァスパラギン酸、アマチヤエキス、ァラニン、アルギニン、ァ ノレギン酸 Na、アルテアエキス、アロエべラエキスー、オイスタエキス、ォォムギバクガ エキス、カキ葉、加水分解ケラチン、加水分解コラーゲン、加水分解コンキォリン、カロ 水分解卵殻膜、加水分解卵白、加水分解シルク、加水分解ダイズタンパク、褐藻ェ キス、カリンエキス、キイチゴエキス、キシリトーノレ、キトサン、キユウリエキス、キユウリ果 実エキス、グアバ菓エキス、クィンスシードエキス、グリシン、グリセリン、グノレコース、 グレープフルーツエキス、グレープフルーツ果実エキス、タレマティスエキス、ゴボウ エキス、コメ発酵液、コンドロイチン硫酸 Na、魚コラーゲ入サンザシエキス、シィタケ エキス、ジォゥエキス、グリセリン、シスチン、システィン、スギナエキス、ゼニァォイエ キス、セリン、ソノレビトーノレ、ダイズタンパク、トマトエキス、乳酸 Na、乳酸桿菌、ダイズ 醱酵エキス、尿素仰/ノバラエキス'アーモンド浬、コーン油、ハチミツ、ヒアルロン酸 Na、フクノエキス、ベタイン、へチマエキス、マノレチトーノレ、マノレトース、マンニトーノレ、 ユリエキス、ラタトフエリン、リシン、リンゴエキス、レンゲソゥエキス、ローヤノレゼリー、ラ ノリン脂肪酸ポリエチレングリコール 1000、ラノリン脂肪酸イソプロピル、ミリスチン酸ィ ソプロピル、ミリスチン酸イソブチル、へキシルデカノール、乳酸ミリスチル、ラノリン脂 肪酸、トリカプリノレグリセリノレ、ォレイルアルコール、ォレイン酸オタチルドデシル、ォレ イン酸デシル、還元ラノリン、オタチルドデカノール、アーモンド油、アボカド油、オリ ーブ油、ォレイン酸、オレンジラフィー油、カカオ β旨、力ロットエキス、ゴマ油、サザン 力油、サフラワー油、ジヒドロコレステロール、スクヮラン、ステアリン酸コレステリル、セ ラミド 2、月見草油、ヒマシ油、ヒマヮリ油、セフニド 3、ヒマヮリ種子油ハイブリッドヒマヮ リ油、フイトスフインゴシン、ブドウ種子油、ホホバ油、ホホバ種子油、ミネラルオイル、 ミンク油、マカデミアナッツ油、メドウフォーム油、ユーリ油、ユーカリ葉油、ラノリン、リ ノール酸、ローズヒップ油、ワセリン及びポリグルタミン酸などが挙げられる。 [0138] As a whitening agent, L-ascorbic acid and its derivatives, hydroquinone and its derivatives, tranexamic acid, lucinol, edetic acid and its derivatives, and placenta extract, t-AMC HA, acerola extract, age extract, ellagic acid or its derivatives, Flaming extract, chamomile extract, force bud flower extract (urea), kiwi extract, glutathione, tocotrienol, ferulic acid, raspberry ketone, noresinol, walurshkis, pyridoxine dipalmitate, io, kojic acid or its derivatives, darcosamine or Derivatives thereof, hydroxy cinnoic acid or derivatives thereof, dartathione, arginium extract, gonon extract, sohakhi extract, psycho extract, boufu extract, garlic mushroom mycelium culture or extract thereof, linden extract, Peach leaf extract, age extract, kujin extract, juju extract, touki Extracts, Yakuyyun Extract, Oyster Leaf Extract, Dai Ou Extract, Buttonpi Extract, Nomadame Extract, Maronier Extract, Hypericum Extract, Oil Soluble Extract, etc. Moisturizers include sodium pyridonecarboxylate, glycol, glycerin, glucose, maltose , Maltitol, sucrose, fructose, xylitonor, sonorebitoren, manoletotriose, threitol, erythritole, amylolytic sugar-reduced alcohol, sonolebithonole, and polyhydric alcohols include ethylene glycol, 1,4-butylene glycol, diglycerin, triglycerin , Tetraglycerin, 1,3-butylene glycol, propylene glycol, dipropylene glycol, polyethylene glycol, 1,3-propanedio , Serine, glycine, threonine, alanine, collagen, hydrolyzed collagen, hydronectin, fibronectin, keratin, elastin, royal jelly, chondroitin sulfate heparin, glyce oral phospholipid, glyce oral glycolipid, sphingophospholipid, sphingo Glycolipid, linoleic acid or its ester, eicosapentaenoic acid or its ester, pectin, bifidobacteria fermentation product, lactic acid fermentation product, yeast extract, litchi mycelium culture or its extract, wheat germ oil, apogado oil, rice germ oil, jojoba oil, soy phospholipids, Ί Orizano Norre, Below de mallow extract, Yotainin'ekisu, Jiouekisu, Taisouekisu, Kaisowe kiss, Kidachiaroe extract, burdock root extract, Ganoderma wax extract, Anoreni force extract, / j, Fusuma, pyrrolidone carboxylic acid, tomato extract, camellia oil, soybean phospholipid, hyaluronic acid, threonine, ammonium glycolate, methyl silanol alginate, otainin, touki extract, toki root extract, dice, extract, asparagus extract, DNA-Na , PCA-Na, RNA-Na, ashitaba extract, aspartate, amatya extract, alanine, arginine, anoreginate Na, artea extract, aloe vera extract, oyster extract, barley leaf extract, oyster leaf, hydrolyzed keratin, hydrolyzed collagen, Hydrolyzed conchiolin, caro Hydrolyzed eggshell membrane, hydrolyzed egg white, hydrolyzed silk, hydrolyzed soy protein, brown algae echex, karin extract, raspberry extract, xylitol, chitosan, cucumber extract, cucumber fruit extract, guava confectionery Vinegar, Quinn scan seed extract, glycine, glycerin, Gunorekosu, grapefruit extract, grapefruit fruit extract, sauce Matisse extract, burdock root extract, fermented rice liquor, chondroitin sulfate Na, fish collagen input hawthorn extract, Shiitake Extract, Zio extract, Glycerin, Cystine, Cystine, Horsetail extract, Zeniaiekis, Serine, Sonorbitonore, Soy protein, Tomato extract, Lactic acid Na, Lactobacillus, Soybean fermented extract, Urea / Novara extract 'Almond mushroom, Corn oil, Honey, Hyaluronic acid Na, fukuno extract, betaine, hechima extract, manoletotonole, manoletos, mannito nore, lily extract, ratatoferrin, ricin, apple extract, lotus root extract, royano jelly, lanolin fatty acid polyethylene glycol 1000, lanolin fatty acid isopropyl, myristic acid isopropyl, myristate Isobutyl, hexyldecanol, myristyl lactate, lanolin fatty acid, tricaprinoleglycerinole, oleyl alcohol, oleate oleate Sil, decyl oleate, reduced lanolin, otatildodecanol, almond oil, avocado oil, olive oil, oleic acid, orange raffie oil, cacao beta, power lot extract, sesame oil, southern power oil, safflower Oil, dihydrocholesterol, squalane, cholesteryl stearate, ceramide 2, evening primrose oil, castor oil, castor oil, cefnide 3, castor seed oil hybrid castor oil, phytosphingosin, grape seed oil, jojoba oil, jojoba seed Examples include oil, mineral oil, mink oil, macadamia nut oil, meadow foam oil, yuri oil, eucalyptus leaf oil, lanolin, linoleic acid, rosehip oil, petrolatum and polyglutamic acid.
[0140] 例えば、微生物培養代謝物としては、酵母エキス、亜鉛含有酵母エキス、ゲルマ二 ゥム含有酵母エキス、セレン含有酵母エキス、マグネシウム含有酵母エキス、米醱酵 エキス、ユーグレナ抽出物、脱脂粉乳の乳酸発酵物などが挙げられる。 [0140] For example, microbial culture metabolites include yeast extract, zinc-containing yeast extract, germanium-containing yeast extract, selenium-containing yeast extract, magnesium-containing yeast extract, rice fermentation extract, Euglena extract, and skim milk powder. Examples include lactic acid fermented products.
[0141] 例えば、 α—ヒドロキシ酸としては、グリコーノレ酸、クェン酸、リンゴ酸、酒石酸、乳 酸などが挙げられる。  [0141] Examples of the α-hydroxy acid include glyconolic acid, citrate, malic acid, tartaric acid, and lactic acid.
[0142] 例えば、無機顔料としては、無水ケィ酸、ケィ酸マグネシウム、タルク、カオリン、ベ ントナイト、マイ力、雲母チタン、ォキシ塩化ビスマス、酸化ジルコニウム、酸化マグネ シゥム、酸化亜鉛、酸化チタン、炭酸カルシウム、炭酸マグネシウム、黄酸化鉄、ベン ガラ、黒酸化鉄、グンジヨウ、酸化クロム、水酸化クロム、カーボンブラック、カラミンな どが挙げられる。  [0142] For example, as inorganic pigments, anhydrous caustic acid, magnesium silicate, talc, kaolin, bentonite, my strength, titanium mica, bismuth oxychloride, zirconium oxide, magnesium oxide, zinc oxide, titanium oxide, calcium carbonate Magnesium carbonate, yellow iron oxide, red iron oxide, black iron oxide, gundiio, chromium oxide, chromium hydroxide, carbon black, calamine and the like.
[0143] 例えば、紫外線吸収剤としては、 ρ—ァミノ安息香酸誘導体、サルチル酸誘導体、 アントラニル酸誘導体、クマリン誘導体、アミノ酸系化合物、ベンゾトリアゾール誘導 体、テトラゾール誘導体、イミダゾリン誘導体、ピリミジン誘導体、ジォキサン誘導体、 カンファー誘導体、フラン誘導体、ピロン誘導体、核酸誘導体、アラントイン誘導体、 ニコチン酸誘導体、ビタミン B6誘導体、ォキシベンゾン、ベンゾフエノン、グアイァズレ ン、シコニン、バイカリン、ノイカレイン、ベルべリンなどが挙げられる。 [0143] For example, as an ultraviolet absorber, ρ-aminobenzoic acid derivative, salicylic acid derivative, anthranilic acid derivative, coumarin derivative, amino acid compound, benzotriazole derivative, tetrazole derivative, imidazoline derivative, pyrimidine derivative, dioxane derivative, Examples include camphor derivatives, furan derivatives, pyrone derivatives, nucleic acid derivatives, allantoin derivatives, nicotinic acid derivatives, vitamin B6 derivatives, oxybenzone, benzophenone, guaiazurene, shikonin, baicalin, noikalein, and berberine.
[0144] 例えば、収斂剤としては、乳酸、酒石酸、コハク酸、クェン酸、アラントイン、塩化亜 鉛、硫酸亜鉛、酸化亜鉛、カラミン、 p—フエノールスルホン酸亜鉛、硫酸アルミニウム カリウム、レソルシン、塩化第二鉄、タンニン酸などが挙げられる。  [0144] For example, as astringents, lactic acid, tartaric acid, succinic acid, succinic acid, allantoin, zinc chloride, zinc sulfate, zinc oxide, calamine, zinc p-phenol sulfonate, potassium aluminum sulfate, resorcin, secondary chloride Examples thereof include iron and tannic acid.
[0145] 抗菌剤として、ォゥバタ抽出液、ハロカルバン、クロロフヱネシン、塩化リゾチーム、塩 酸アルキルジアミノエチルグリシン、イソプロピルメチルフエノール、チモール、へキサ クロ口フェン、ベルべリン、チォキソロン、サリチル酸およびそれらの誘導体、安息香 酸、安息香酸ナトリウム、パラォキシ安息香酸エステル、パラクロルメタクレゾール、塩 化ベンザルコニゥム、フエノキシエタノール、イソプロピルメチルフエノール、石炭酸、 ソルビン酸、ソルビン酸カリウム、へキサクロ口フェン、塩化クロルへキシジン、トリクロ口 カルバニリド、感光素、ビス（2—ピリジノレチォー 1ーォキシド）亜鉛、チアントール、ヒノ キチォ一ノレ、トリクロサン、トリクロロヒドロキシジフエニノレエーテノレ、クロノレへキシジン ダルコン酸塩、フエノキシエタノール、レゾルシン、ァズレン、サリチル酸、ジンクピリチ オン、モノニトログアヤコールナトリウム、ウイキヨウエキス、サンショウエキス、塩化セチ ルピリジニゥム、塩化べンゼトニゥム及びゥンデシレン酸誘導体などが挙げられる。  [0145] As an antibacterial agent, ovata extract, halocarban, chlorophenesine, lysozyme chloride, alkyldiaminoethylglycine hydrochloride, isopropylmethylphenol, thymol, hexacyclophene, berberine, thixolone, salicylic acid and their derivatives, Benzoic acid, sodium benzoate, paraoxybenzoic acid ester, parachloromethcresol, benzalkonium chloride, phenoxyethanol, isopropylmethylphenol, carboxylic acid, sorbic acid, potassium sorbate, hexaclonal fenphene, chlorhexidine chloride, trichloro Oral carbanilide, photosensitizer, bis (2-pyridinorethio 1-oxide) zinc, thianthol, hinokitiol, triclosan, trichlorohydroxydiphenenoleethenole, chronorehexidine Rukon salts, phenoxyethanol, resorcinol, Azuren, salicylic, Jinkupirichi on, mononitroguayacol sodium, Huy dexterity extract, pepper extract, chloride cetirizine Rupirijiniumu, such as downy chloride Nzetoniumu and Undeshiren acid derivatives.
[0146] 例えば、殺菌 '消毒薬としては、アタリノール、ィォゥ、塩化ベンザルコニゥム、塩化 ベンゼトニゥム、塩化メチルロザ二リン、クレゾ一ノレ、ダルコン酸カルシウム、ダルコン 酸クロルへキシジン、スルファミン、マーキュロクロム、ラタトフエリンまたはその加水分 解物などが挙げられる。  [0146] For example, as a disinfectant, atalinol, io, benzalkonium chloride, benzethonium chloride, methyl rosaniline chloride, cresol monole, calcium dalconate, chlorhexidine dalconate, sulfamine, mercurochrome, latatoferrin or its addition Moisture breakdown products.
[0147] 例えば、頭髪用剤としては、二硫化セレン、臭化アルキルイソキノリニゥム液、ジンク ピリチ才ン、ビフエナミン、チアントール、カスタリチンキ、ショウキヨウチンキ、トウガラシ チンキ、塩酸キニーネ、強アンモニア水、臭素酸カリウム、臭素酸ナトリウム、チォダリ コール酸などが挙げられる。  [0147] For example, as a hair preparation, selenium disulfide, alkylisoquinolinium bromide, zinc pilichn, bifuenamine, thianthol, castari tincture, pepper tincture, pepper tincture, quinine hydrochloride, strong ammonia water, Examples include potassium bromate, sodium bromate, and thiodaricholic acid.
[0148] 例えば、香料としては、ジヤコゥ、シベット、カストリウム、アンバーグリスなどの天然 動物性香料、ァニス精油、アングリ力精油、イラン精油、ィリス精油、ウイキヨゥ精油、 オレンジ精油、カナンガ精油、力ラウエ一精油、カルダモン精油、グアヤタウッド精油 、クミン精油、黒文字精油、ケィ皮精油、シンナモン精油、ゲラニゥム精油、コパイバ バルサム精油、コリアンデル精油、シソ精油、シダーウッド精油、シトロネラ精油、ジャ スミン精油、ジンジャーグラス精油、杉精油、スペアミント精油、西洋ハツ力精油、大茴 香精油、チュべローズ精油、丁字精油、橙花精油、冬緑精油、トルーバルサム精油、 バチユリ一精油、バラ精油、パルマローザ精油、檜精油、ヒバ精油、白檀精油、プチ ダレン精油、べィ精油、べチバ精油、ベルガモット精油、ペルーバルサム精油、ボア ドローズ精油、芳樟精油、マンダリン精油、ユーカリ精油、ライム精油、ラベンダー精 油、リナロェ精油、レモングラス精油、レモン精油、ローズマリー精油、和種ハツ力精 油などの植物性香料、その他合成香料などが挙げられる。 [0148] For example, as a fragrance, natural animal fragrances such as Jiakou, Civet, Castrium, Ambergris, Anise essential oil, Angri essential oil, Iran essential oil, Iris essential oil, Wikiyo essential oil, Orange essential oil, Kananga essential oil, Power Laue essential oil , Cardamom essential oil, guayata wood essential oil , Cumin essential oil, black letter essential oil, key skin essential oil, cinnamon essential oil, geranium essential oil, copaiba balsam essential oil, coriandel essential oil, perilla essential oil, cedarwood essential oil, citronella essential oil, jasmine essential oil, gingergrass essential oil, cedar essential oil, spearmint essential oil, Western Hearts Power essential oil, Otsuki perfume essential oil, tuberose essential oil, clove essential oil, orange flower essential oil, winter green essential oil, trout balsam essential oil, Baturi lily essential oil, rose essential oil, palmarosa essential oil, cocoon essential oil, hiba essential oil, sandalwood essential oil, petit darren essential oil , Essential oil, vetiva essential oil, bergamot essential oil, peru balsam essential oil, bored rose essential oil, mellow essential oil, mandarin essential oil, eucalyptus essential oil, lime essential oil, lavender essential oil, linaloe essential oil, lemongrass essential oil, lemon essential oil, rosemary essential oil , Vegetable flavors such as Japanese beetroot oil, other synthetic flavors, etc. And the like.
[0149] 例えば、色素 ·着色剤としては、赤キャベツ色素、赤米色素、ァカネ色素、アナトー 色素、イカスミ色素、ゥコン色素、ェンジュ色素、ォキアミ色素、柿色素、カラメル、金 、銀、クチナシ色素、コーン色素、タマネギ色素、タマリンド色素、スピルリナ色素、ソ バ全草色素、チェリー色素、海苔色素、ハイビスカス色素、ブドウ果汁色素、マリーゴ 一ルド色素、紫ィモ色素、紫ャマイモ色素、ラック色素、ルチンなどが挙げられる。  [0149] For example, as a colorant / colorant, a red cabbage dye, a red rice dye, an akane dye, an anato dye, a squid ink dye, a turquoise dye, an enji dye, a krill dye, an amber dye, a caramel, a gold, a silver, a gardenia dye, Corn pigment, onion pigment, tamarind pigment, spirulina pigment, buckwheat whole plant pigment, cherry pigment, laver pigment, hibiscus pigment, grape juice pigment, marigo mulberry pigment, purple imo pigment, purple potato pigment, lac pigment, rutin, etc. Is mentioned.
[0150] 例えば、甘味料としては、砂糖、甘茶、果糖、ァラビノース、ガラクトース、キシロース 、マンノース、麦芽糖、蜂蜜、ブドウ糖、ミラクリン、モネリンなどが挙げられる。  [0150] Examples of sweeteners include sugar, sweet tea, fructose, arabinose, galactose, xylose, mannose, maltose, honey, glucose, miraculin, monelin and the like.
[0151] 例えば、栄養強化剤としては、貝殻焼成カルシウム、シァノコラバミン、酵母、小麦 胚芽、大豆胚芽、卵黄粉末、へミセルロース、ヘム鉄などが挙げられる。  [0151] For example, examples of the nutrient fortifier include shell calcined calcium, cyanobacamine, yeast, wheat germ, soybean germ, egg yolk powder, hemicellulose, heme iron and the like.
[0152] 育毛剤としては、ペンタデカン酸グリセリド、コレウスエキス、ゲンチアナエキス、マッカ サエキス、ローヤノレゼリーエキス、クマザサエキス、 t-フラバノン、 6-ペンジノレアミノプリ ン、センブリエキス、塩化カルプロニゥム、ミノキシジル、フィナステリド、アデノシン、二 コチン酸アミド、桑の根エキス、ジォゥエキス、 5-アミノレブリン酸などが挙げられる。  [0152] As the hair restorer, pentadecanoic acid glyceride, coleus extract, gentian extract, macca extract, roya jelly extract, kumazasa extract, t-flavanone, 6-penzinoreaminopurine, assembly extract, carpronium chloride, minoxidil, finasteride , Adenosine, nicotinamide, mulberry root extract, dio extract, and 5-aminolevulinic acid.
[0153] 動植物抽出物としては、ァセンャク（阿仙薬）、ァシタバ、ァセロラ、アルテア、アル二 力、アボカド、アマチヤ (甘茶）、アロエ、アロエベラ、イラクサ、イチヨウ (銀杏葉，銀杏） 、ウイキヨゥ（茴香）、ゥコン (鬱金）、ウスパサイシン (細辛）、ウメ（烏梅）、ゥラジ口ガシ、 ゥワウルシ、ノイバラ（営実）、ヒキォコシ (延命草）、ォウギ (黄耆）、コガネバナ (ォゥゴ ン）、ャマザクラ (桜皮）、キハダ (黄柏）、ォゥレン (黄連）、ォタネニンジン (人参）、オト ギリソゥ（弟切草）、ォドリコソゥ、オランダガラシ、オレンジ、イトヒメハギ（遠志）、ゥッボ ダサ (夏枯草）、ツルドクダミ (何首烏）、ェンジュ (槐花）、ョモギ (ガイ葉）、ガジュッ（ 莪朮）、クズ (葛根）、カノコソゥ（吉草根）、力ミツレ、キカラスゥリ（瓜呂根）、力ワラョモ ギ（茵チン蒿）、カンゾゥ（甘草）、フキタンポポ (款冬花，款冬葉）、キイチゴ、キウイ果 実、キキヨウ (桔梗）、キク (菊花）、キササゲ (梓実）、ミカン属植物果実 (枳実）、タチバ ナ (橘皮)、キユウリ、ウドまたはシシウド (羌活，独活）、アンズ (杏仁）、クコ（地骨皮，枸 杞子，枸杞葉）、クララ（苦参）、タスノキ、クマザサ、グレープフルーツ果実、ュッケィ（ 桂皮）、ケィガイ (ケィガイ）、ェビスダサ (決明子）、マルバアサガオ又はアサガオ (ケ ン牛子）、ベニバナ（紅花）、ゴバイシ (五倍子）、コンフリー、コパイバ、クチナシ（山梔 子）、ゲンチアナ、ホオノキ (厚朴）、ヒナタイノコズチ（牛膝）、ゴシュュ（呉茱萸）、ゴボ ゥ、チョウセンゴミシ (五味子）、米、米ぬか、コムギ、ミシマサイコ（柴胡）、サフラン、サ ボンソゥ、サンザシ（山ザ子）、サンショウ（山椒）、サルビア、サンシチニンジン（三七 人参）、シィタケ (椎茸）、ジォゥ（地黄）、シクンシ (使君子）、ムラサキ (紫根)、シソ (紫 蘇葉，紫蘇子）、カキ (柿蒂）、シャクャク (芍薬）、ォォバコ（車前子，車前草）、ショウ ガ（生姜）、ショウブ（菖蒲）、トウネズミモチ（女貞子）、シモッケソゥ、シラカバ、スイカ ズラ（金銀花，忍冬）、セィヨウキヅタ、セィヨウノコギリソゥ、セィヨウニヮトコ、ァズキ（ 赤小豆）、ニヮトコ (接骨木）、ゼニァオイ、センキユウ (川キユウ）、センプリ（当薬）、ク ヮ（桑白皮，桑葉）、ナツメ（大棗）、ダイズ、タラノキ、チクセッニンジン (竹節人参）、 ハナスゲ (知母）、ヮレモコゥ（地楡）、ドクダミ（十薬）、フユムシナツクサタケ (冬虫夏 草）、トウガラシ、ホオズキ (登呂根）、タチジヤコゥソゥ、リヨクチャ（緑茶）、コウチヤ（紅 茶）、チヨウジ (丁子）、ゥンシユウミカン（陳皮）、ツバキ、ッボクサ、トウガラシ (番椒）、 トウキ（当帰）、トウキンセン力、ダイダイ (橙皮）、ヮレモコゥ（地楡）、トウモロコシ (南蛮 毛）、トチュウ（杜仲，杜仲葉）、トマト、ナンテン (南天実）、ユンユタ (大サン）、ォォム ギ (麦芽）、ハクセン（白蘚皮）、ジヤノヒゲ (麦門冬）、パセリ、バタタ、ハツ力（薄荷）、 ハマメリス、バラ、ビヮ葉 (枇杷葉）、マツホド（茯リヨウ）、ブドウまたはその葉、へチマ、 ボダイジュ、ボタン (牡丹皮）、ホップ、マイカイ（マイ瑰花）、松葉、マロニエ、マンネン ロウ、ムクロジ、メリッサ、メリロート、ボケ (木瓜）、モヤシ、モモ（桃仁，桃葉）、ヒォウギ (射干）、ビンロウジュ (檳ロウ子）、メハジキ（益母草）、ャグルマギク、ユキノシタ（虎耳 草）、ャマモモ（楊梅皮）、ヤシャブシ（矢車）、ノ、トムギ（ョクイニン）、モウコョモギ、ャ マョモギ、ラベンダー、リンゴ果実、マンネンタケ（霊芝）、レモン果実、レンギヨゥ（連 翹）、レンゲソゥ、ゲンノショウコ（老鸛草）、ハシリドコロ（ロート根）、鶏トサ力、牛.人の 胎盤抽出物、豚'牛の胃、十二指腸、或いは腸の抽出物若しくはその分解物、水溶 性コラーゲン、水溶性コラーゲン誘導体、コラーゲン加水分解物、エラスチン、エラス チン加水分解物、水溶性エラスチン誘導体、シルク蛋白、シルク蛋白分解物、牛血 球蛋白分解物などが挙げられる。 [0153] Animal and plant extracts include Asenjak (Asen Yaku), Ashitaba, Acerola, Altea, Al Niki, Avocado, Amachiya (Amacha), Aloe, Aloe Vera, Nettle, Ichiyo (Ginkgo Leaves, Ginkgo), Wikiyo (Ayaka) , Turmeric (Usukin), Uspasaishin (spicy), Japanese apricot (Plum), Vulture mouth oak, mulberry, Neubara (Nonjutsu), hikikoshi (life-prolonging grass), ogi (Twilight), Koganebana (Ogon), yamazakura (cherry skin) ), Kihada (Yellow), Oulen (Huang Ren), Ginseng (Ginseng), Oto Girisou (Little Cut Grass), Odorico Sou, Dutch Garashi, Orange, Itohimehagi (Distance), Tubo Dasa (Summer Blossom), Tsurudokudami (What's Necklace), Yenju (Sweet Flower), Momogi (Gai Leaf), Gajutsu (Kaki), Kuzu (Kazune), Kanokosou (Valentine Root), Force Mitsule, Kikarasuri (Gurone), Power Warabimogi (bamboo chin), licorice (licorice), licorice poppo (section winter flower, section winter leaf), raspberry, kiwi fruit, kikiyou (kikyo), chrysanthemum (chrysanthemum flower), yellow sage (fruit) ), Tachibana (Tachibana bark), Japanese cucumber, Udo or Shishiudo (Akihito, Akihito), Apricot (Apricot jelly), Cucumber (Bone husk, Samurai coconut, Bamboo leaf), Clara (Bitter), Tasoki, Kumazasa, Grapefruit Fruit, Ukkei, Keyai, Yebisuda (Keriko), Malva morning glory or morning glory (Ken beef), safflower (safflower), Gobaishi (penta), Comfrey, Copaiba, Cutina (Yamasuko), Gentiana, Honoki (Koh-Pak), Hinata Taikozuchi (cow knee), Goshu (Kurean), Gobo, Korean ginger (Gomiko), Rice, Rice bran, Wheat, Mishima Saiko (Saihu), Saffron, Sabon Sou, Hawthorn (Yamazako), Salamander, Salvia, Sancynin carrot (37 carrots), Shitake (Shibai), Jioh (geo yellow), Sikunshi (Amiko), Murasaki (Purple), Shiso ( Shiso leaves, shisoko), oysters (柿蒂), peonies (glaze), psyllium (car front child, car in front of grass), ginger (ginger), shobu (、), gerbil mochi (Sadako), Shimokesou, birch, Watermelon Zula (Golden Silver Flower, Shinobu Winter), Seyio Ivy, Seyoko Sawtooth, Seyi Yoneko, Azuki (Red Azuki), Niytoko (Bonewood), Zeniaoi, Senkiyu (River Kiyu) , Senpuri (Drug), ク ヮ (mulberry white skin, mulberry leaf), jujube (Daisatsu), soybean, taranoki, Chikusenninjin (Bamboo ginseng), Hanasuge (knowing mother), ヮ Remokou (district), Dokudami ( 10 medicines), Fuyumushinatsusatake (Cycium caterpillar), capsicum, physalis (Torone), Tachijiyakousou, ryokuku (green tea), kouchiya (crimson tea), chiyouji (clove), seaweed mikan (Chinese skin), camellia, dillosa, Capsicum (Sanban), Touki (Toshiki), Tokinsen force, Daidai (Orange peel), Tomo Remokou (gem), Corn (Nantou), Tomato, Nanten (Nan Tenji), Yun Utah (Large sun), omugi (malt), hakusen (white birch bark), jiyanohige (barley winter), parsley, scallops, heart force (thin load), hamamelis, roses, bibi (leaf), matsuhod (Lily), grape or its leaves, loofah, bodaiju, button (peony skin), hops, maikai (my buds), pine needles, marronnier, mannen wax, mukuroji, melissa, melirot, bokeh (trees), sprout, Peach (Peach seed, Peach leaf), Panther (spouted), Betel-rouge (Camellia), Mejijiki (beneficial herb), Pomegranate, Yukinoshita (Tiger ear grass), Prunus (Plum), Yashabushi (Yagar), No, Tomagi (Yokuinin) , Moukomomogi Mugwort, lavender, apple fruit, garlic mushroom (reishi), lemon fruit, forsythia, rengesou, gennoshouko (old-growth grass), ashridokoro (roth root), chicken tossa power, cattle. Human placenta extract, pig 'Bovine stomach, duodenum, or intestinal extract or its degradation product, water-soluble collagen, water-soluble collagen derivative, collagen hydrolyzate, elastin, elastin hydrolysate, water-soluble elastin derivative, silk protein, silk protein degradation And bovine blood cell protein degradation products.
[0154] 紫外線吸収剤として、安息香酸ァミル、パラアミノ安息香酸ォクチル、サリチル酸ェチ レングリコール、サリチル酸フエニル、サリチル酸ォクチル、サリチル酸ベンジル、サリ チル酸ブチルフエニル、サリチル酸ホモメンチル、ケィ皮酸ベンジル、パラメトキシケ ィ皮酸 2—エトキシェチル、ノ ラメトキシケィ皮酸ォクチル、ジパラメトキシケィ皮酸モ ノ 2—ェチルへキサン酸グリセリル、パラメトキシケィ皮酸イソプロピル、ジイソプロピル 'ジイソプロピルケイ皮酸エステル混合物、ゥロカニン酸、ゥロカニン酸ェチル、ヒドロ キシメトキシベンゾフエノン、ヒドロキシメトキシベンゾフエノンスルホン酸およびその塩 、ジヒドロキシメトキシベンゾフエノン、ジヒドロキシメトキシベンゾフエノンジスルフォン 酸ナトリウム、ジヒドロキシベンゾフエノン、テトラヒドロキシベンゾフエノン、 4— tert— ブチルー 4 'ーメトキシージベンゾィルメタン、 2、 4、 6 トリア二リノ p— (カルポー 2 ' ェチルへキシルー 1 ' ォキシ） 1、 3、 5 トリアジン、 2-(2-ヒドロキシ -5-メチルフ ェニル）ベンゾトリァゾール、パラジメチルァミノ安息香酸 2-ェチルへキシル、パラアミ ノ安息香酸ェチル、トプチルメトキシジペンゾィルメタン、ォキシベンゾン - 1、グアイァ ズレンスルホン酸ェチル、酸化亜鉛、シノキサートなどが挙げられる。  [0154] As an ultraviolet absorber, amyl benzoate, octyl paraaminobenzoate, ethylene glycol salicylate, phenyl salicylate, octyl salicylate, benzyl salicylate, butylphenyl salicylate, homomenthyl salicylate, benzyl quine, paramethoxykelate 2 —Ethoxyethyl, normethoxykelate octyl, diparamethoxykelate mono-2-ethyl glyceryl hexanoate, isopropyl paramethoxykelate, diisopropyl'diisopropylcinnamate ester mixture, urocanic acid, urocanic acid ethyl, hydroxy Methoxybenzophenone, hydroxymethoxybenzophenonesulfonic acid and its salts, dihydroxymethoxybenzophenone, dihydroxymethoxybenzophenone sodium disulfonate, Hydroxybenzophenone, Tetrahydroxybenzophenone, 4— tert—Butyl-4′-methoxydibenzoylmethane, 2, 4, 6 Trianilino p— (Carpo 2 ”Ethylhexylone 1′oxy) 1, 3 , 5 Triazine, 2- (2-hydroxy-5-methylphenyl) benzotriazole, 2-dimethylethyl paradimethylaminobenzoate, Ethyl paraaminobenzoate, Topylmethoxydipenzylmethane, Oxybenzone- 1, guaizulene sulfonate ethyl, zinc oxide, sinoxate and the like.
[0155] 抗酸化剤としては、ァスコルビン酸及びその塩、ステアリン酸エステル、トコフエロール 及びそのエステル誘導体、ノルジヒドログァセレテン酸、ブチルヒドロキシトルエン（B HT)、ブチルヒドロキシァ二ソール（BHA)、パラヒドロキシァニソール、没食子酸プロ ピル、セサモール、セサモリン、ゴシポールなどが挙げられる。  [0155] Antioxidants include ascorbic acid and its salts, stearic acid ester, tocopherol and its ester derivatives, nordihydroguasertenic acid, butylhydroxytoluene (BHT), butylhydroxyanisole (BHA), para Examples include hydroxyanisole, propyl gallate, sesamol, sesamorin, gossypol.
[0156] 抗炎症剤としては、グリチルリチン酸及びその塩、グリチルレチン酸及びその塩、イソ プロピルアミノカプロン酸及びその塩、アラントイン、塩化リゾチーム、グアイァズレン、 サリチル酸メチル、 γ—オリザノール、抗酸化剤としては、 αカロチン、 13カロチン、 yカロチン、リコピン、クリプトキサンチン、ノレティン、ゼアキサンチン、イソゼアキサン チン、ロドキサンチン、カプサンチン、クロセチン等のカロチノイド； 1 , 4ージァザシクロ オクタン、 2, 5—ジメチルフラン、 2—メチルフラン、 2, 5—ジフエ二ルフラン、 1 , 3— ジフエニノレイソべンゾフラン、 αトコフェローノレ、 /3トコフェローノレ、 γトコフェローノレ、 d トコフエロール、ヒスチジン、トリプトファン、メチォニン、ァラニン又はそのアルキルエス テノレ；ジブチルヒドロキシトルエン、ブチルヒドロキシァニソール、ァスコルビン酸、タン ニン酸、ェピカテキン、ェピカロカテキン、ェピカテキンガレート、ェピカロ力テキンガ レート等のタンニン類、ルチン等のフラボノイド、その他没食子酸プロピル、ァスタキ サンチン、カロチン、トコフエロール、ァスコルビン酸、ェデト酸四ナトリウム、エリソル ビン酸、酢酸トコフエロール、酢酸レチノール、ジビチルヒドロキシトルエン、ステアリン 酸ァスコルビル、パルミチン酸ァスコルビル、メチルシラノールジォレイルトコフェロー ル '無水ケィ酸混合物、ニコチン酸ベンジル、感光素 401号、ァスパラギン酸、アデノ シン三リン酸 2Na [0156] Anti-inflammatory agents include glycyrrhizic acid and its salts, glycyrrhetinic acid and its salts, isopropylaminocaproic acid and its salts, allantoin, lysozyme chloride, guaizlene, methyl salicylate, γ-oryzanol, and antioxidants include α Carotene, 13 carotene, y carotene, lycopene, cryptoxanthine, noretin, zeaxanthin, isazexane Carotenoids such as tin, rhodoxanthine, capsanthin, crocetin; 1,4-diazacyclooctane, 2,5-dimethylfuran, 2-methylfuran, 2,5-diphenylfuran, 1,3-dipheninoreisobenzofuran, α-tocopherol , / 3 tocopherol, γ tocopherol, d tocopherol, histidine, tryptophan, methionine, alanine or its alkyl ester; dibutylhydroxytoluene, butylhydroxyanisole, ascorbic acid, tannic acid, epicatechin, epicarocatechin , Tannins such as epicatechin gallate and epicarote techin gallate, flavonoids such as rutin, propyl gallate, astaxanthin, carotene, tocopherol, ascorbic acid, tetrasodium edetate, erythorbium Acid, tocopherol acetate, retinol acetate, dibityl hydroxytoluene, ascorbyl stearate, ascorbyl palmitate, methylsilanoldiolatecoferol 'mixture of carboxylic acid anhydride, benzyl nicotinate, photosensitizer 401, aspartic acid, adenosine Triphosphate 2Na
、ァミノ酪酸、ウイキヨウエキス、オランダ力ラシエキス、カフェイン、クロレラエキス、サ フランエキス、ショウキヨウエキス、ダイズエキス、タイソゥエキス、葉酸、レチノール、パ ルミチン酸レチノ一ノレ、イノシトーノレ、ゥコンエキス、オリザノーノレ、カロチン、力ロットェ キス、コムギ ¾牙エキス、センキユウエキス、チンピエキス、トウキエキス、トウキ根ェキ ス、ドダミエキス、トコフェローノレ、ニコチン酸トコフェローノレ、ボタンエキス、エノレゴカ ルシフエロール、ジカプリル酸ピリドキシン、バチルアルコール、ステアリン酸ダリチル レチュル、グリチルリチン酸、グリチルレチン酸、塩酸ジフェンヒドラミン、塩化リゾチ一 ム、アミノカプロン酸、レイシエキス、ョタイニン、メリロートエキス、ボタンエキス、トウキ エキス、トウキ根エキス、センキユウエキス、ゲンノショコエキス、アラントイン、ァノレニ力 エキス、ァノレニ力花エキス、ォゥゴンエキス、ォゥレンエキス、ォドリコソゥエキス、ガマ 穂エキス、力ミツレエキス、カラミン、力ワラョモギエキス、甘草エキス、グアイァズレン、 クチナシエキス、クマザサエキス、グリチルリチン酸 2K、グリチルレチン酸ステアリル、 ゲンチアナエキス、紅茶エキス、コンフリーエキス、コンフリー葉エキス、酢酸トコフエ ロール、サリチル酸メチル、酸化亜鉛、シコンエキス，ムラサキ根エキス、シソエキス、 シソ葉エキス、シモッケソゥエキス、シャクャクエキス、スィカズラエキス、セージエキス 、セィヨウキズタエキス、セィヨウニヮトコエキス、セィヨウノコギリソゥエキス、センブリエ キス、ソゥハクヒエキス、トウキンセン力エキス、ピリドキシン HC1、ビヮ葉エキス、フユボ ダイジュエキス、モモ葉.果実エキス、ャグルマギクエキス、ユキノシタエキス、ョモギ エキス、レタスエキス、ローマ力ミツレエキス、ヮレモコゥエキス及び力カローノレ、ポリア ミンなどが挙げられる。 , Aminobutyric acid, wikiweed extract, Dutch power pear extract, caffeine, chlorella extract, saffron extract, ginger extract, soybean extract, taisou extract, folic acid, retinol, retinoic acid palmitate, inositole, turmeric extract, oryzanol, carotene, force Lotte kiss, wheat ¾ tusks extract, citrus extract, chimpi extract, toki extract, toki root extract, dodami extract, tocopherol, tocopherol nicotinate, button extract, enolegocalciferol, pyridoxine dicaprylate, batyl alcohol, stearic acid Darityl lettuce, glycyrrhizic acid, glycyrrhetinic acid, diphenhydramine hydrochloride, lysozyme chloride, aminocaproic acid, litchi extract, otatin, melilot extract, button Extract, Toki Extract, Toki Root Extract, Senkiyu Extract, Gennoshoko Extract, Allantoin, Anoleni Force Extract, Anoleni Force Flower Extract, Ogon Extract, Oren Extract, Odorikoso Extract, Gama Ear Extract, Force Mitsuru Extract, Calamine, Force Waragigi Extract, Licorice extract, guaiazulene, gardenia extract, kumazasa extract, glycyrrhizic acid 2K, stearyl glycyrrhetinate, gentian extract, tea extract, comfrey extract, comfrey leaf extract, tocopherol acetate, methyl salicylate, zinc oxide, cicon extract, purple root extract, Perilla extract, Perilla leaf extract, Shimokke-so extract, Peonies extract, Cypricot extract, Sage extract, Syringa extract, Syona nitoco extract, Syeo mushroom soe Vinegar, Senburie Kiss, Sohakuhi extract, Tokinsen extract, Pyridoxine HC1, Biyaku leaf extract, Fuyubo Daiju extract, Peach leaf. Min etc. are mentioned.
[0157] その他、ホルモン類、金属イオン封鎖剤、 pH調整剤、キレート剤、防腐'防バイ剤、 清涼剤、安定化剤、乳化剤、動 ·植物性蛋白質およびその分解物、動 ·植物性多糖 類およびその分解物、動'植物性糖蛋白質およびその分解物、血流促進剤、消炎剤 *抗アレルギー剤、細胞賦活剤、角質溶解剤、創傷治療剤、増泡剤、増粘剤、口腔 用剤、消臭 ·脱臭剤、苦味料、調味料、酵素などが挙げられる。  [0157] Others, hormones, sequestering agents, pH adjusters, chelating agents, antiseptics, antibacterial agents, fresheners, stabilizers, emulsifiers, animal / plant proteins and their degradation products, animal / plant polysaccharides And its degradation products, animal and plant glycoproteins and their degradation products, blood flow promoters, anti-inflammatory agents * Anti-allergic agents, cell activators, keratolytic agents, wound treatment agents, foam enhancers, thickeners, oral cavity Preparations, deodorants / deodorants, bitters, seasonings, enzymes, etc.
[0158] 本発明の第 1の発明に係る活性酸素消去剤は、外用剤として、化粧品、医薬部外 品、医療用品、衛生用品、医薬品に配合して実施することができる。 [0158] The active oxygen scavenger according to the first invention of the present invention can be used as an external preparation by blending it into cosmetics, quasi drugs, medical supplies, hygiene products, and pharmaceuticals.
本発明の第 1の発明に係る活性酸素消去剤を外用剤として、化粧品、医薬部外品 、医療用品、衛生用品、医薬品、飲食品の形態で実施する場合、剤形は限定されず 、アンプル、カプセル、粉末、顆粒、丸剤、錠剤、固形剤、液剤、ゲル、気泡、乳液、 クリーム、軟膏、シート、ムース、浴用剤など多様なものとすることができる。  When the active oxygen scavenger according to the first invention of the present invention is used as an external preparation in the form of cosmetics, quasi-drugs, medical supplies, hygiene products, pharmaceuticals, foods and drinks, the dosage form is not limited, and ampoules , Capsules, powders, granules, pills, tablets, solids, solutions, gels, bubbles, emulsions, creams, ointments, sheets, mousses, bath preparations, and the like.
[0159] 化粧品、医薬部外品、医薬品の形態で実施する場合、外用剤とすることが好適で ある。また、食用とされているキク科植物の抽出物は経口摂取も可能であるため、外 用剤に限定されず、内用剤、飲食品としてもよい。 [0159] When implemented in the form of cosmetics, quasi-drugs, and pharmaceuticals, it is preferable to use external preparations. In addition, the extracts of the Asteraceae plants that are considered to be edible can be taken orally, so they are not limited to external preparations but may be internal preparations and foods and drinks.
[0160] 具体的には、化粧品、医薬部外品、医療用品、衛生用品、医薬品としては、例え ば内用'外用薬用製剤、化粧水、乳液、クリーム、軟膏、ローション、オイル、パックな どの基礎化粧料、洗顔料や皮膚洗浄料、シャンプー、リンス、ヘアートリートメント、へ ァクリーム、ポマード、ヘアスプレー、整髪料、パーマ剤、ヘアートニック、染毛料、育 毛'養毛料などの頭髪化粧料、ファンデーション、白粉、おしろい、口紅、頰紅、アイ シャドウ、アイライナー、マスカラ、眉墨、まつ毛などのメークアップ化粧料、美爪料な どの仕上げ用化粧料、香水類、浴用剤、その他、歯磨き類、口中清涼剤'含嗽剤、液 臭 ·防臭防止剤、衛生用品、衛生綿類、ウエットティシュなどが挙げられる。より具体 的には、化粧石鹼、シャンプー、洗顔料、リンス、アイクリーム、アイシャドウ、クリーム' 乳液、化粧水、香水、おしろい、化粧油、頭髪用化粧品、染毛料、練香水、パウダー 、 ノ ック、クレンジングクリーム、ひげそり用クリーム、びげそり用ローション、 日焼けォ ィノレ、 日焼け止め才ィノレ、 日焼けローション、 日焼け止めローション、 日焼けクリーム、 日焼け止めクリーム、ファンデーション、粉末香水、ほお紅、マスカラ、眉墨、爪タリー ム、美爪エナメル、美爪エナメル除去液、洗毛料、浴用化粧品、口紅、リップクリーム 、アイライナー、歯磨き、デオドラント剤、オーデコロン、養毛剤および育毛剤などとし て使用すること力できる。また、本発明の活性酸素消去剤を含有する化粧品組成物 は、軟膏剤や湿布剤などとして使用することもできる。 [0160] Specifically, cosmetics, quasi-drugs, medical products, hygiene products, and pharmaceuticals include, for example, internal use external preparations, lotions, emulsions, creams, ointments, lotions, oils, packs, etc. Basic cosmetics, facial cleansers and skin cleansers, shampoos, rinses, hair treatments, hair creams, pomades, hair sprays, hair straighteners, permanents, hair nicks, hair dyes, hair growth cosmetics, foundations , White powder, funny, lipstick, scarlet, eye shadow, eyeliner, mascara, eyebrows, eyelashes and other makeup cosmetics, beauty nails, and other finishing cosmetics, perfumes, bath preparations, toothpaste, in the mouth Examples include refreshing agents, gargles, liquid odors, deodorants, sanitary products, sanitary cotton, and wet tissue. More specifically, cosmetic stone shampoo, shampoo, face wash, rinse, eye cream, eye shadow, cream 'milky lotion, lotion, perfume, funny, cosmetic oil, hair cosmetics, hair dye, perfume, powder , Knock, cleansing cream, shave cream, beard shave lotion, sunscreen, sunscreen, sunscreen lotion, sunscreen lotion, sun cream, sunscreen cream, foundation, powdered perfume, blusher, mascara, eyebrow It can be used as a nail cream, nail enamel, nail enamel remover, hair wash, bath cosmetic, lipstick, lip balm, eyeliner, toothpaste, deodorant, eau de cologne, hair nourishing agent and hair restorer. The cosmetic composition containing the active oxygen scavenger of the present invention can also be used as an ointment or a poultice.
[0161] 飲食品類としては、例えば清涼飲料、炭酸飲料、栄養飲料、果実飲料、乳酸飲料等 の飲料、アイスクリーム、アイスシャーベット、力、き氷等の冷菓、そば、うどん、はるさめ 、ぎょうざの皮、しゅうまいの皮、中華麵、即席麵等の麵類、飴、キャンディー、ガム、 チョコレート、錠菓、スナック菓子、ビスケット、ゼリー、ジャム、クリーム、焼き菓子、パ ン等の菓子類、力二、サケ、アサリ、マグロ、イワシ、ェビ、カツォ、サノ 、クジラ、カキ、 サンマ、イカ、ァカガイ、ホタテ、ァヮビ、ゥュ、イクラ、トコブシ等の水産物、力、まぼこ、 ハム、ソーセージ等の水産 ·畜産加工食品、加工乳、発酵乳等の乳製品、サラダ油、 てんぷら油、マーガリン、マヨネーズ、ショートニング、ホイップクリーム、ドレッシング等 の油脂及び油脂加工食品、ソース、たれ等の調味料、カレー、シチュー、親子丼、お 粥、雑炊、中華丼、かつ丼、天丼、うな丼、ハヤシライス、おでん、マーボドーフ、牛丼 、ミートソース、玉子スープ、オムライス、餃子、シユーマイ、ノヽンノ ーグ、ミートボーノレ 等のレトルトバウチ食品、種々の形態の健康 ·栄養補助食品、保健機能食品、錠剤、 カプセル剤、ドリンク剤、トローチなどが挙げられる。  [0161] Examples of the foods and drinks include soft drinks, carbonated drinks, nutrition drinks, fruit drinks, lactic acid drinks, ice cream, ice sherbet, power, frozen ice confectionery, soba, udon, harusame, gyoza skin , Sweet potato skins, Chinese rice cakes, instant rice cakes, rice cakes, candy, gum, chocolate, tablets, snacks, biscuits, jelly, jams, creams, baked goods, pans, etc. , Seafood such as clams, tuna, sardines, shrimp, bonito, sano, whales, oysters, saury, squid, akagai, scallops, arabi, yu, ichura, tokobushi, and other marine products such as power, maboko, ham, sausage · Dairy products such as processed livestock foods, processed milk, fermented milk, salad oil, tempura oil, margarine, mayonnaise, shortening, whipped cream, dressing, etc. Fat and oil processed foods, seasonings such as sauces, sauces, curry, stew, oyakodon, rice cakes, miscellaneous cooking, Chinese rice bowl, bonito, tempura, eel rice, hayashi rice, oden, mabodorf, beef bowl, meat sauce, egg soup, Examples include retort bouch foods such as omelet rice, dumplings, shiyumai, non-nog, meat bonore, various forms of health / nutritional supplements, health functional foods, tablets, capsules, drinks, troches and the like.
[0162] 本発明の第 1の発明に係る活性酸素消去剤は、ヒトに対して好適に適用されるもの であるが、それぞれの作用効果が期待できる限り、ヒト以外の動物に対して適用する ことあでさる。  [0162] The active oxygen scavenger according to the first invention of the present invention is preferably applied to humans, but is applied to animals other than humans as long as each effect can be expected. That's it.
[0163] 本発明の第 1の発明の活性酸素消去剤は、特に限定されるものではないが、非ィ オン性の界面活性剤や低級アルコール、多価アルコール、あるいはォリーブ油、スク ヮラン、脂肪酸などの天然油脂に溶解して用いることができる。  [0163] The active oxygen scavenger of the first invention of the present invention is not particularly limited, but nonionic surfactant, lower alcohol, polyhydric alcohol, olive oil, squalane, fatty acid It can be used by dissolving in natural fats and oils.
[0164] 非イオン界面活性剤としては、例えば、ソルビタン脂肪酸エステル類 (例えば、ソル ビタンモノォレエート、ソルビタンモノイソステアレート、ソルビタンモノラウレート、ソル ビタンモノパルミテート、ソルビタンモノステアレート、ソルビタンセスキォレエート、ソ ノレビタントリオレエート、ペンター 2—ェチルへキシル酸ジグリセロールソルビタン、テ トラー 2—ェチルへキシル酸ジグリセロールソルビタン等）；グリセリンポリグリセリン脂 肪酸類 (例えば、モノ綿実油脂肪酸グリセリン、モノエル力酸グリセリン、セスキォレイ ン酸グリセリン、モノステアリン酸グリセリン、 α , α，ーォレイン酸ピログルタミン酸ダリ セリン、モノステアリン酸グリセリンリンゴ酸等）；プロピレングリコール脂肪酸エステル 類（例えば、モノステアリン酸プロピレングリコール等）；硬化ヒマシ油誘導体；グリセリ ンアルキルエーテル等が挙げられる。 [0164] Nonionic surfactants include, for example, sorbitan fatty acid esters (for example, sorbitan monooleate, sorbitan monoisostearate, sorbitan monolaurate, sorbitan monolaurate). Vitan monopalmitate, sorbitan monostearate, sorbitan sesquioleate, sonorebitane trioleate, pentater 2-diethylhexyl glycerol disorbate sorbitan, tetra 2-hexyl hexyl diglycerol sorbitan, etc.); glycerin polyglycerin Fatty acids (for example, mono cottonseed oil fatty acid glycerin, monoergic acid glycerin, glyceryl sesquioleate, glyceryl monostearate, α, α, -oleic acid pyroglutamate dariserin, monostearic glycerin malic acid, etc.); propylene glycol fatty acid ester (For example, propylene glycol monostearate); hardened castor oil derivative; glycerin alkyl ether and the like.
ΡΟΕ系の親水性非イオン界面活性剤としては、例えば、 ΡΟΕ—ソルビタン脂肪酸ェ ステル類（例えば、 ΡΟΕ—ソルビタンモノォレエート、 ΡΟΕ—ソルビタンモノステアレ ート、 ΡΟΕ—ソルビタンモノォレート、 ΡΟΕ—ソルビタンテトラオレエート等）； ΡΟΕソ ルビット脂肪酸エステル類（例えば、 ΡΟΕ—ソルビットモノラウレート、 ΡΟΕ—ソルビッ トモノォレエート、 ΡΟΕ—ソルビットペンタォレエート、 ΡΟΕ—ソルビットモノステアレ ート等）； ΡΟΕ—グリセリン脂肪酸エステル類（例えば、 ΡΟΕ—グリセリンモノステアレ ート、 ΡΟΕ—グリセリンモノイソステアレート、 ΡΟΕ—グリセリントリイソステアレート等 の ΡΟΕ—モノォレエート等）； ΡΟΕ—脂肪酸エステル類（例えば、 ΡΟΕ—ジステアレ ート、 ΡΟΕ—モノジォレエート、ジステアリン酸エチレングリコール等）； ΡΟΕ—アルキ ノレエーテル類（例えば、 ΡΟΕ—ラウリルエーテル、 ΡΟΕ—ォレイルエーテル、 ΡΟΕ ーステアリルエーテル、 ΡΟΕ—べへニルエーテル、 ΡΟΕ— 2—オタチルドデシルェ 一テル、 ΡΟΕ—コレスタノールエーテル等）；プル口ニック型類（例えば、プル口ニック 等）； ΡΟΕ· POP—アルキルエーテル類（例えば、 ΡΟΕ· POP—セチルエーテル、 P OE · POP— 2—デシルテトラデシルエーテル、 POE · POP—モノブチルエーテル、 POE'POP—水添ラノリン、 POE'POP—グリセリンエーテル等）；テトラ POE.テトラ POP—エチレンジァミン縮合物類（例えば、テトロニック等）； POE—ヒマシ油硬化ヒ マシ油誘導体（例えば、 POE—ヒマシ油、 POE—硬化ヒマシ油、 POE—硬化ヒマシ 油モノイソステアレート、 POE—硬化ヒマシ油トリイソステアレート、 POE—硬化ヒマシ 油モノピログルタミン酸モノイソステアリン酸ジエステル、 POE—硬化ヒマシ油マレイ ン酸等）； POE—ミツロウ'ラノリン誘導体（例えば、 POE—ソルビットミツロウ等）；アル 力ノールアミド（例えば、ヤシ油脂肪酸ジエタノールアミド、ラウリン酸モノエタノールァ ミド、脂肪酸イソプロパノールアミド等）； POE プロピレングリコール脂肪酸エステル ； POE -アルキルアミン； POE 脂肪酸アミド；ショ糖脂肪酸エステル；アルキルエト キシジメチルアミンォキシド；トリオレィルリン酸等が挙げられる。 Examples of the hydrophilic hydrophilic nonionic surfactant include 、 -sorbitan fatty acid esters (for example, ΡΟΕ-sorbitan monooleate, ΡΟΕ-sorbitan monostearate, ΡΟΕ-sorbitan monooleate, ΡΟΕ —Sorbit fatty acid esters (eg, ΡΟΕ—Sorbit monolaurate, ΡΟΕ—Sorbit monooleate, ソ ル —Sorbit pentaoleate, ΡΟΕ—Sorbit monostearate, etc.); Glycerin fatty acid esters (eg, ΡΟΕ-glycerol monostearate, ΡΟΕ-glycerol monoisostearate, ΡΟΕ-monooleate, etc.); ΡΟΕ-fatty acid esters (eg, ΡΟΕ-distearate) , ΡΟΕ—Monoziエ ー ト -alkynole ethers (e.g., ΡΟΕ-lauryl ether, ΡΟΕ-oleyl ether, ΡΟΕ-stearyl ether, ΡΟΕ-behenyl ether, ΡΟΕ-2-octyldodecyl ether, ΡΟΕ-cholestanol ether, etc.); pull mouth nick type (eg, pull mouth nick, etc.); ΡΟΕ · POP-alkyl ethers (eg, ΡΟΕ · POP-cetyl ether, P OE · POP-2-decyl tetradecyl ether) POE · POP-monobutyl ether, POE'POP-hydrogenated lanolin, POE'POP-glycerin ether, etc.); Tetra POE. Tetra POP-ethylene diamine amine condensates (for example, Tetronic, etc.); POE-castor oil-cured castor Oil derivatives (eg POE—castor oil, POE—hardened castor oil, POE—hardened castor oil monoisoss) AOETO, POE—hardened castor oil triisostearate, POE—hardened castor oil monopyroglutamic acid monoisostearic acid diester, POE—hardened castor oil maleic acid, etc.); POE—honey bees' lanolin derivatives (eg, POE—sorbite beeswax etc.) ); Al Powered olamide (eg coconut oil fatty acid diethanolamide, lauric acid monoethanolamide, fatty acid isopropanolamide, etc.); POE propylene glycol fatty acid ester; POE-alkylamine; POE fatty acid amide; sucrose fatty acid ester; alkyl ethoxydimethylamine oxide Trioleyl phosphate and the like.
[0166] 低級アルコールとしては、例えば、エタノール、プロパノール、イソプロパノール、イソ ブチルアルコール、 t ブチルアルコール等が挙げられる。  [0166] Examples of the lower alcohol include ethanol, propanol, isopropanol, isobutyl alcohol, t-butyl alcohol and the like.
[0167] 多価アルコールとしては、例えば、 2価のアルコール（例えば、エチレングリコール、 プロピレングリコール、トリメチレングリコール、 1 , 2—ブチレングリコール、 1 , 3—ブチ レングリコーノレ、テトラメチレングリコーノレ、 2, 3 ブチレングリコーノレ、ペンタメチレン グリコーノレ、 2 ブテン 1 , 4 ジォーノレ、へキシレングリコーノレ、オタチレングリコー ル等）；3価のアルコール（例えば、グリセリン、トリメチロールプロパン等）；4価アルコ ール（例えば、 1 , 2, 6 へキサントリオール等のペンタエリスリトール等）； 5価アルコ ール（例えば、キシリトール等）；6価アルコール（例えば、ソノレビトーノレ、マンニトーノレ 等）；多価アルコール重合体（例えば、ジエチレングリコール、ジプロピレングリコール 、トリエチレングリコール、ポリプロピレングリコール、テトラエチレンダリコール、ジグリ セリン、ポリエチレングリコール、トリグリセリン、テトラグリセリン、ポリグリセリン等）；2価 のアルコールアルキルエーテル類（例えば、エチレングリコールモノメチルエーテル、 エチレングリコーノレモノェチノレエーテノレ、エチレングリコーノレモノブチノレエーテノレ、ェ チレングリコ一ノレモノフエニノレエーテノレ、エチレングリコ一ノレモノへキシノレエーテノレ、 エチレングリコーノレモノ 2—メチノレへキシノレエーテノレ、エチレングリコーノレイソアミノレェ ーテノレ、エチレングリコーノレべンジノレエーテノレ、エチレングリコーノレイソプロピノレエ一 テノレ、エチレングリコーノレジメチノレエーテノレ、エチレングリコーノレジェチノレエーテノレ、 エチレングリコールジブチルエーテル等）； 2価アルコールアルキルエーテル類（例え ば、ジエチレングリコーノレモノメチノレエーテノレ、ジエチレングリコーノレモノェチノレエー テル、ジエチレングリコールモノブチルエーテル、ジエチレングリコールジメチルエー テノレ、ジエチレングリコーノレジェチノレエーテノレ、ジエチレングリコーノレブチノレエーテ ノレ、ジエチレングリコーノレメチノレエチノレエーテノレ、トリエチレングリコーノレモノメチノレエ ーテノレ、トリエチレングリコーノレモノェチノレエーテノレ、プロピレングリコーノレモノメチノレ エーテノレ、プロピレングリコーノレモノェチノレエーテノレ、プロピレングリコ一ノレモノブチ ノレエーテノレ、プロピレングリコーノレイソプロピノレエーテノレ、ジプロピレングリコ一ノレメチ ノレエーテノレ、ジプロピレングリコーノレエチノレエーテノレ、ジプロピレングリコーノレブチノレ エーテル等）；2価アルコールエーテルエステル（例えば、エチレングリコールモノメチ ルエーテルアセテート、エチレングリコールモノェチルエーテルアセテート、エチレン グリコーノレモノブチノレエーテノレアセテート、エチレングリコーノレモノフエニノレエーテノレ アセテート、エチレングリコーノレジアジべート、エチレングリコーノレジサクシネート、ジ エチレングリコールモノェチルエーテルアセテート、ジエチレングリコールモノブチル エーテノレアセテート、プロピレングリコーノレモノメチノレエーテノレアセテート、プロピレン グリコーノレモノェチノレエーテノレアセテート、プロピレングリコーノレモノプロピノレエーテ ルアセテート、プロピレングリコールモノフエニルエーテルアセテート等）；グリセリンモ ノアルキルエーテル（例えば、キミノレァノレコーノレ、セラキルアルコール、バチルアルコ ール等）；糖アルコール（例えば、ソノレビトーノレ、マルチトーノレ、マノレトトリオース、マン 二トーノレ、ショ糖、エリトリトーノレ、グルコース、フルクトース、デンプン分解糖、マルトー ス、キシリトース、デンプン分解糖還元アルコール等）；グリソリッド；テトラハイド口フル フリノレアノレコーノレ； POE テトラハイド口フルフリルアルコール； POP ブチルエーテ ノレ； POP · POE ブチノレエーテノレ；トリポリォキシプロピレングリセリンエーテノレ； POP -グリセリンエーテル； POP -グリセリンエーテルリン酸； POP · POE -ペンタンエリス リトールエーテル、ポリグリセリン等が挙げられる。 [0167] Examples of the polyhydric alcohol include dihydric alcohols (for example, ethylene glycol, propylene glycol, trimethylene glycol, 1,2-butylene glycol, 1,3-butylene glycolanol, tetramethylene glycolanol, 2, 3 Butylene glycolanol, pentamethylene glycolanol, 2 butene 1,4 dianol, hexylene glycolanol, octylene glycol, etc .; Trivalent alcohol (eg, glycerin, trimethylolpropane, etc.); Tetravalent alcohol (eg, 1, 2, 6 hexanetriol, etc. pentaerythritol, etc.); pentavalent alcohol (eg, xylitol, etc.); hexavalent alcohol (eg, sonorebitanol, mannitol, etc.); polyhydric alcohol polymer (eg, diethylene glycol, diethylene glycol, etc.) Propylene glycol Ethylene glycol, polypropylene glycol, tetraethylenedaricol, diglycerin, polyethylene glycol, triglycerin, tetraglycerin, polyglycerin, etc.); divalent alcohol alkyl ethers (eg, ethylene glycol monomethyl ether, ethylene glycol monomethenole) Etherenole, ethylene glycol monobutinole etherenole, ethylene glycol mononomonomono etherenole, ethylene glycol mononomonohexenoreethenore, ethylene glyconoremono 2-methylenohexenoreethenore, ethylene glyconoreisoamino Retenore, Ethyleneglycolenozenoreethenore, Ethyleneglycolenoisopropynoleate, Tenole, Ethyleneglycolenoresinechinoreetenore, Ethi Di-glycol alcohol monoether ether, diethylene glycol monobutyl ether, diethylene glycol monobutyl ether, diethylene glycol monobutyl ether, diethylene glycol dibutyl ether, diethylene glycol monobutyl ether, diethylene glycol monobutyl ether, diethylene glycol Dimethyl glycolate, diethylene glycolateletenoleatenore, diethyleneglycololebutinoreatenore, diethyleneglycolenolemethinoreethinoreatenore, triethyleneglycolenomonomethinoreatenore, triethyleneglycolenolemonotinore Tenoré, propylene glycolenomonomonochinole Ethereol, Propylene glycol monoreino enoate, Propylene glycol monoremonobutenore, Propylene glycol monoisopropinoreate nore, Dipropylene glycol monoremethinoreate nore, Dipropylene glyconoreino enoreateore, Dipropylene glyconore noetinore ether, etc. ); Dihydric alcohol ether esters (for example, ethylene glycol monomethyl ether acetate, ethylene glycol monoethyl ether acetate, ethylene glyconomonomonobutylenoate acetate, ethylene glycol monomonoinoatenoate acetate, ethylene glyconoresazia) Bait, ethyleneglycolenoresuccinate, diethylene glycol monoethyl ether acetate, diethylene Recall monobutyl etherate acetate, propylene glycol monomethinoate etherate acetate, propylene glyconomonoethylenoate acetate, propylene glycol monopropinoate acetate, propylene glycol monophenyl ether acetate, etc.); glycerin mono Alkyl ethers (for example, minomino ethanol, cerakil alcohol, batyl alcohol, etc.); sugar alcohols (for example, sonorebitole, multitonole, manoletotriose, mannitol, sucrose, erythritole, glucose, fructose, starch degradation Sugar, maltose, xylitol, amylolytic sugar-reduced alcohol, etc.); Glysolid; Tetrahide Mouth Full Flenorea Noreconore; POE Tetra High Oral furfuryl alcohol; POP Butyl ether Nole; POP · POE Butinole etherate; Tripolyoxypropylene glycerol etherol; POP-Glycerine ether; POP-Glycerine ether phosphate; POP · POE-Pentane erythritol ether, polyglycerol etc. Can be mentioned.
[0168] 油類としては、アボカド油、ォリーブ油、ゴマ油、ツバキ油、月見草油、タートル油、 マ力デミアンナッツ油、トウモロコシ油、ミンク油、ナタネ油、卵黄油、パーシック油、小 麦胚芽油、サザン力油、ヒマシ油、アマ二油、サフラワー油、綿実油、エノ油、大豆油 、落花生油、茶実油、カャ油、コメヌ力油、キリ油、ホホバ油、カカオ脂、ヤシ油、馬油 、パーム油、パーム核油、牛脂、羊脂、豚脂、ラノリン、鯨ロウ、ミツロウ、カルナウバロ ゥ、モクロウ、キャンデリラロゥ、スクヮラン等の動植物油及びその硬化油。流動バラフ イン、ワセリン等の鉱物油、トリパルミチン酸グリセリン等の合成トリグリセリンがある。  [0168] As oils, avocado oil, olive oil, sesame oil, camellia oil, evening primrose oil, turtle oil, madame demian nut oil, corn oil, mink oil, rapeseed oil, egg yolk oil, persic oil, oat germ oil , Southern power oil, castor oil, Amani oil, safflower oil, cottonseed oil, Eno oil, soybean oil, peanut oil, tea seed oil, cocoa oil, rice bran oil, kiri oil, jojoba oil, cocoa butter, coconut oil, Animal and vegetable oils such as horse oil, palm oil, palm kernel oil, beef tallow, sheep fat, pork tallow, lanolin, whale wax, beeswax, carnauba roux, moclaw, candelilla roux, squalane, and hardened oils thereof. There are fluidized paraffin, mineral oil such as petrolatum, and synthetic triglycerin such as glycerin tripalmitate.
[0169] 高級脂肪酸としては、例えばラウリン酸、ミリスチン酸、ノ ルミチン酸、ォレイン酸、リ ノール酸、リノレン酸、ステアリン酸、ベヘン酸、 12—ヒドロキシステアリン酸、イソステ アリン酸、ゥンデシン酸、トール酸、エイコサペンタエン酸、ドコサへキサェン酸などが ある。高級アルコールとしては、例えば、ラウリノレアノレコール、セチルアルコール、ステ ァリノレアノレ 一ノレ、ベへニノレアノレコーノレ、ミリスチノレアノレ: 3—ノレ、才レイノレアノレコーノレ 、セトステアリノレアノレコーノレ、ホホバァノレコーノレ、ラノリンァノレコーノレ、ノ チノレアノレコー ノレ、 2—デシノレテトラテセシノーノレ、コレステロ一ノレ、フィトステロ一ノレ、イソステアリノレ アルコール等がある。合成エステルとしては、例えば、オクタン酸セチル、ミリスチン酸 オタチルドデシル、ミリスチン酸イソプロピル、ミリスチン酸ミリスチル、パルミチン酸イソ プロピル、ステアリン酸プチル、ラウリン酸へキシル、オレンィ酸デシル、ジメチルォク タン酸、乳酸セチル、乳酸ミリスチル等がある。シリコーンとしては、例えば、ジメチル ポリシロキサン、メチノレフエニノレポリシロキサン等の鎖状ポリシロキサン、デカメチノレシ クロポリシロキサン等の環状ポリシロキサン、シリコーン樹脂等の三次元網目構造のも の等がある。 [0169] Examples of higher fatty acids include lauric acid, myristic acid, normitic acid, oleic acid, linoleic acid, linolenic acid, stearic acid, behenic acid, 12-hydroxystearic acid, isostere. Examples include aspartic acid, undecinic acid, tolic acid, eicosapentaenoic acid, docosahexaenoic acid. Examples of the higher alcohols include laurinorenolechol, cetyl alcohol, stearinorenole monole, beheninoleanoreconole, myristinoleanore: 3-nore, reino reinorenorenore, ceto stearinorenoreconole, Jojobano Reconole, Lanolinano Reconole, Notino Reano Renore, 2-Desino Rete Tethecinore, Cholesterol Nore, Phytosterol Nore, Isostearinore Alcohol, etc. Synthetic esters include, for example, cetyl octanoate, octyldodecyl myristate, isopropyl myristate, myristyl myristate, isopropyl palmitate, butyl stearate, hexyl laurate, decyl orenate, dimethyl octanoate, cetyl lactate, Examples include myristyl lactate. Examples of the silicone include chain polysiloxanes such as dimethyl polysiloxane and methinorefinole polysiloxane, cyclic polysiloxanes such as decamethylenocyclopolysiloxane, and three-dimensional network structures such as silicone resins.
[0170] 次に、本発明の第 2の発明を実施するための最良の形態について説明する。  Next, the best mode for carrying out the second invention of the present invention will be described.
[0171] 本明細書において「賦活化」とは、細胞機能や細胞活性を維持または亢進させるこ とが意図される。その結果、細胞機能や細胞活性の低下を抑制、すなわち細胞の老 化を抑制すること力 Sできる。したがって、「賦活化剤」は「細胞賦活剤」と同義であり、「 抗老化剤」としての有用性を持つ。 [0171] As used herein, "activation" is intended to maintain or enhance cell function or activity. As a result, it is possible to suppress a decrease in cell function and cell activity, that is, to suppress cell aging. Therefore, “activator” is synonymous with “cell activator” and has utility as “anti-aging agent”.
[0172] 例えば、皮膚細胞における「賦活化、抗老化」とは、加齢や光老化による基低膜の 構造変化の蓄積に伴う皮膚細胞の機能低下を小さくすることで、皮膚のしわ、たるみ 、硬化等を防止、改善して弾力のある若々しい健康な肌の状態を維持することなどを 指している。 [0172] For example, “activation, anti-aging” in skin cells refers to reducing wrinkles and sagging of the skin by reducing the deterioration of the function of skin cells associated with the accumulation of structural changes in the substrata membrane due to aging and photoaging. This refers to the prevention and improvement of curing, etc., and maintaining the skin's elasticity and youthful healthy skin.
[0173] 本発明の第 2の発明に係る賦活化剤は、キク科植物の抽出物を有効成分として含 有するものであればよぐ中でもコゥモリソゥ属植物の抽出物を有効成分として含有 するものが好ましい。  [0173] The activator according to the second invention of the present invention is not particularly limited as long as it contains an extract of an Asteraceae plant as an active ingredient, and among them, an activator contains an extract of a genus Camellia plant as an active ingredient. preferable.
[0174] キク科植物としては、キク属（Dendranthema)、シォン属（Aster)、キンセンカ属（Calen dula)、ェゾギク属 (Callistephus)、ベニバナ属 (Carthamus)、コスモス属 (Cosmos)、 ダリァ属（Dahlia)、ガザニァ属（Gazania)、ガーベラ属（Gerbera)、ヒマヮリ属（Helianth us)、セネシォ属（Senecio)、ジニァ属（Zinnia)、ノコギリソゥ属 (Achillea)、アグラタム 属 Ageratum)、カイザイク属 (Ammobium)、ヒナギク属 (Bellis)、ャグノレマソゥ属（Centa urea)、ァザミ属 (Cirsium)、コレオプシス属 (Coreopsis)、ディモノレフォセォ属 (Dimorp hotheca)、ェキノプス属（Echinops)、フェリシア属 (Felicia)、ガイラルディア属（Gaillar dia)、ミヤコヮスレ属 (Gymnaster)、ムギワラギク属（Helichrysum)、ローダンセ属 (Heli pterum)、リアトリス属 (Liatris)、ルドべキア属（Rudbeckia)、ストケシァ属（Stokesia)、 タゲテス属（Tagetes)、コゥモリソゥ属 (Cacalia)、ォォモミジガサ属（Miricacalia)、ャブ レガサ属（Syneilesis)などが挙げられる力 その中でも、コゥモリソゥ属力 S望ましい。 [0174] The Asteraceae plants include the genus Dendranthema, Aster, Calend dula, Callistephus, Carthamus, Cosmos, and Dahlia. ), Gazania, Gerbera, Helianth us, Senecio, Zinnia, Achillea, Agratam Genus Ageratum), Kaizoic genus (Ammobium), Daisies (Bellis), Cageno urea, Cirsium, Coreopsis, Dimorp hotheca, Echinops , Felicia, Gaillar dia, Gymnaster, Helichrysum, Rholiperum, Liatris, Rudbeckia, Strokesia (Stokesia), Tagetes genus (Cacalia), moss genus (Miricacalia), genus Syneilesis, etc. Among them, bat genus power S is desirable.
[0175] コゥモリソゥ属植物としては、コゥモリソゥ、モミジガサ、力ニコゥモリ、タマブキ、ィヌド ゥナ、ィズカニコゥモリ、ォォカニコゥモリ、タイミンガサ、ックシコゥモリソゥ、テバコモミ ジガサ、ニシノヤマタイミンガサ、ミミコゥモリ、モミジコゥモリ、ヤマタィミンガサ、ョブス マソゥなどが挙げられる。その中でも、食用植物が好ましぐモミジガサが適している。  [0175] The plants of the genus Komorisou include bat Morisou, Momijigasa, Nikkomori, Tamabuki, Innuduna, Izkanikomori, Okanikomori, Taimingasa, Kushikomorisou, Tebacomomijigasa, Nishinoyama Taimingasa, Mikamimori Etc. Among them, Momijigasa, which is preferred by edible plants, is suitable.
[0176] モミジガサは、別名、シドケ、シドキ、シドキナ、シンドキ、キノシタ、トウキチ、トウキチ ナ、スドケ、シトギ、スドケと呼ばれており、キク科の植物で日本国内に広く分布し、山 菜として食されている。  [0176] Momijigasa is also known as Sidke, Sidki, Sidkina, Shindoki, Kinoshita, Toukichi, Tokichina, Sudoke, Sitgi, Sudoke, and is a asteraceae plant widely distributed in Japan and eaten as a wild vegetable. Has been.
[0177] 本発明の第 2の発明に用いられる抽出物は、キク科植物の植物体全体を原料とし て得ることができる。また、植物体を大きぐ根茎、茎、葉と分別してそれぞれの部分 力、ら抽出物を得ることも可能であり、それぞれの部分を適宜組み合わせたものを原料 として抽出物を得てもよい。本発明者らは、モミジガサの根、茎、葉のうち、特に葉に 有効成分が多く存在することを見出しているので、葉を原料として抽出物を得ること が好ましい。  [0177] The extract used in the second invention of the present invention can be obtained using the whole plant of Asteraceae as a raw material. It is also possible to separate the plant body from large rhizomes, stems, and leaves to obtain each partial force and extract, and the extract may be obtained using a combination of each part as a raw material. Since the present inventors have found that among the roots, stems, and leaves of Momijigasa, particularly the leaves have many active ingredients, it is preferable to obtain an extract from the leaves as raw materials.
[0178] キク科植物の植物体は、生の状態、凍結された状態、凍結乾燥された状態のいず れを用いてもよい。好ましくは、生の状態である。生または凍結されたキク科植物から 抽出物を調製する場合には、まず、植物体を破砕してもよい。植物体の破砕にはミキ サー、ホモジナイザー、乳鉢などを用いることができる。凍結乾燥されたものは、凍結 乾燥操作後、粉状に破砕してもよい。  [0178] The plant of the Asteraceae plant may be used in a raw state, a frozen state, or a lyophilized state. Preferably, it is in a raw state. When preparing an extract from a raw or frozen asteraceae plant, the plant body may be first crushed. A mixer, homogenizer, mortar, etc. can be used for crushing plants. The freeze-dried product may be crushed into powder after the freeze-drying operation.
[0179] 植物体から抽出物を得るには溶媒を用いればよい。溶媒を用いる抽出方法として は、室温、冷却または加熱した状態で含浸させて抽出する方法、水蒸気蒸留などの 蒸留法を用いて抽出する方法、圧搾して抽出物を得る圧搾法、超臨界流体または亜 臨界流体を用いて抽出する方法などを好適に用いることができ、これらの方法を単独 で、または 2種以上を組み合わせて抽出を行うことができる。好ましくは、破砕した原 料 lgに対して、 5〜； 100mlの溶媒を用い、 1時間から 1か月間、好ましくは 1〜5日間 、室温あるいは加熱下で抽出を行う。 [0179] A solvent may be used to obtain an extract from a plant. The extraction method using a solvent includes a method of extraction by impregnation in a cooled or heated state at room temperature, a method of extraction using a distillation method such as steam distillation, a compression method of obtaining an extract by pressing, a supercritical fluid or Asia Methods such as extraction using a critical fluid can be suitably used, and these methods can be used alone or in combination of two or more. Preferably, extraction is performed at room temperature or under heating for 1 to 1 month, preferably 1 to 5 days, using 5 to 100 ml of solvent for crushed raw material lg.
[0180] 抽出に用いる溶媒は、有効成分が効果的に抽出される溶媒であれば特に限定され るものではないが、水、またはエタノール等の低級アルコールのように水と混和する 有機溶媒、またはそれらの混液、または酢酸ェチル等の水と混和しない有機溶媒が 挙げられる。具体的には、例えば、メタノール，イソプロパノール，イソブタノール， n— へキサノール，メチルァミルアルコール， 2—ェチルブタノ一ノレ， n—ォクチルアルコ ール等の 1価アルコール類；グリセリン，エチレングリコール，エチレングリコールモノメ チノレエーテノレ，プロピレングリコーノレ，プロピレングリコーノレモノメチノレエーテノレ，プロ ピレングリコーノレモノェチノレエーテノレ， トリエチレングリコーノレ， 1 , 3—ブチレングリコ 一ノレ， へキシレングリコール等の多価アルコールまたはその誘導体；アセトン，メチル ェチルケトン，メチルイソブチルケトン，メチルー n—プロピルケトン等のケトン類；酢酸 イソプロピル等のエステル類；ェチルエーテル，イソプロピルエーテル， n—ブチルェ 一テル等のエーテル類；スクヮラン，ワセリン，パラフィンワックス，パラフィン油などの 炭化水素類;ォリーブ油，小麦胚芽油，米油，ゴマ油，マ力ダミアンナッツ油，アルモ ンド油，ヤシ油等の植物油脂；牛脂，豚脂，鯨油等の動物油脂などが例示される。ま た、リン酸緩衝生理食塩水等の無機塩類を添加した極性溶媒、界面活性剤を添加し た溶媒を用いることもでき、特に限定されない。飲食物等への使用、皮膚への塗布を 含む人体への適用における安全性等を考えれば、特に、水および/またはアルコー ルの!/、ずれかにより抽出することが好まし!/、。  [0180] The solvent used for the extraction is not particularly limited as long as the active ingredient can be effectively extracted, but is water, an organic solvent miscible with water, such as a lower alcohol such as ethanol, or A mixed solution thereof, or an organic solvent immiscible with water such as ethyl acetate can be mentioned. Specifically, monohydric alcohols such as methanol, isopropanol, isobutanol, n-hexanol, methylamyl alcohol, 2-ethylbutanol and n-octyl alcohol; glycerin, ethylene glycol, ethylene glycol mono A polyhydric alcohol such as methinorethenole, propylene glycolenole, propylene glycolenomonomethinoleatenore, propyleneglycolenomonoethylenoreatenore, triethyleneglycolenole, 1,3-butyleneglycolinole, hexylene glycol or the like Derivatives; Ketones such as acetone, methyl ethyl ketone, methyl isobutyl ketone, and methyl-n-propyl ketone; Esters such as isopropyl acetate; Ethyl ether, isopropyl ether, n-butyl ether, etc. Ethers; hydrocarbons such as squalene, petrolatum, paraffin wax, paraffin oil; vegetable oils such as olive oil, wheat germ oil, rice oil, sesame oil, mackerel damian nut oil, almond oil, coconut oil; beef fat, pig Examples thereof include animal fats such as fat and whale oil. In addition, a polar solvent to which inorganic salts such as phosphate buffered saline are added and a solvent to which a surfactant is added can be used, and there is no particular limitation. Considering safety for use in food and drink, application to the human body including application to the skin, etc., it is particularly preferable to extract with water and / or alcohol! /.
[0181] また、段階的に抽出操作を行ってもよい。例えば、エタノール等の低級アルコール のように水と混和する有機溶媒で抽出操作を行い、エバポレーターなどで濃縮し、酢 酸ェチル等の水と混和しない有機溶媒と水とを加え、有機溶媒層と水層に分離して あよい。  [0181] The extraction operation may be performed step by step. For example, extraction is performed with an organic solvent that is miscible with water, such as a lower alcohol such as ethanol, the mixture is concentrated with an evaporator, and an organic solvent that is immiscible with water, such as ethyl acetate, and water are added. May be separated into layers.
[0182] このようにして得られたキク科植物の抽出物は、そのまま用いることもできる力 その 効果を失わない範囲で、脱臭や脱色などの精製処理を加えてもよい。また、抽出物 中の有効成分の濃度を高めるために、得られた抽出物を、さらに濃縮、液液分配、吸 着クロマトグラフィー、順相もしくは逆相クロマトグラフィー等の手段に付すことも可能 である。 The extract of the Asteraceae plant thus obtained may be used as it is, and may be subjected to a purification treatment such as deodorization and decolorization as long as the effect is not lost. Also extract In order to increase the concentration of the active ingredient therein, the obtained extract can be further subjected to means such as concentration, liquid-liquid distribution, adsorption chromatography, normal phase or reverse phase chromatography.
[0183] 例えば、キク科植物を凍結乾燥し、塩化メチレンとメタノール 1： 1の混液 500mlで 抽出し、得られた抽出物をへキサン：酢酸ェチル = 20： 1を用!/、てシリカゲルカラムク 口マトグラフィ一で精製してもよレ、。  [0183] For example, freeze-dry Asteraceae plants and extract with 500 ml of a mixture of methylene chloride and methanol 1: 1, and use the resulting extract with hexane: ethyl acetate = 20: 1! /, Silica gel column You can refine it with the mouthmatography.
[0184] これらの抽出物やその精製物または分画物は、これらから溶媒を除去することによ つて乾固物とすることもでき、さらに、アルコールなどの溶媒に可溶化した形態、ある いは乳剤の形態で用いることがで る。 [0184] These extracts, purified products or fractions thereof may be dried to solids by removing the solvent from these extracts, and may also be in a form solubilized in a solvent such as alcohol. Can be used in the form of an emulsion.
[0185] キク科植物がコゥモリソゥ属のモミジガサである場合、抽出物の主成分は、式 (I) [0185] When the Asteraceae plant is the genus Compositae, the main component of the extract is the formula (I)
[0186] [化 2] [0186] [Chemical 2]
Figure imgf000040_0001
Figure imgf000040_0001
[0187] で表される構造を有する力力ロールである。 [0187] A force roll having a structure represented by:
[0188] 力力ロールは主にキク科植物に含まれていることが知られており、モミジガサ以外に 、 Cacana adenostyloides^ Cacalia  [0188] Power rolls are known to be mainly contained in the Asteraceae plants, in addition to Momigasa, Cacana adenostyloides ^ Cacalia
auriculata、 Ligularia virgaureaに含まれていることが報告されている。また、これら以 外の植物（例えば、 Psacalium It is reported to be contained in auriculata and Ligularia virgaurea. In addition, more than these Outside plants (eg Psacalium)
radulifolium)にも含まれて!/、ることが報告されて!/、る。  radulifolium)! /, reported to be! /
[0189] 本発明者らは、モミジガサ抽出物の力力ロール画分に強い細胞賦活作用があること を見出している。したがって、本発明に係る賦活化剤は力力ロールを有効成分として 含有することが好ましい。抽出物中の力力ロール濃度は、例えば実施例 1に記載のよ うに HPLCを用いて測定することができる。 [0189] The present inventors have found that the powerful roll fraction of the maple extract has a strong cell activation effect. Therefore, the activator according to the present invention preferably contains a power roll as an active ingredient. The strength roll concentration in the extract can be measured using HPLC as described in Example 1, for example.
[0190] 有効成分である力力ロールの含量を高めた精製品、あるいは有効成分である力カロ ール純品としても、本発明の第 2の発明に係る賦活化剤の有効成分として好適に用 いること力 Sでさる。 [0190] A refined product with an increased content of a power roll as an active ingredient or a pure power carol product as an active ingredient is also suitable as an active ingredient of the activator according to the second invention of the present invention. Use force S.
[0191] また、力力ロールはタレゾールメチルエステルを原料として化学合成することができ る（Chem. Lett., 1317 (1975)を参照）。このように化学合成により得られる力力ロール も本発明に係る賦活化剤の有効成分として好適に用いることができる。  [0191] In addition, the power roll can be chemically synthesized using talesol methyl ester as a raw material (see Chem. Lett., 1317 (1975)). Thus, the power roll obtained by chemical synthesis can also be suitably used as an active ingredient of the activator according to the present invention.
[0192] 上述のようにして得られるキク科植物の抽出物、力力ロールの含量を高めた精製品 、または力力ロール純品は、そのまま賦活化剤として利用することも可能である力 化 粧品、医薬部外品、医薬品、飲食品に配合して組成物の形態で実施することが好ま しい。キク科植物の抽出物を配合する濃度は、吸収程度、作用程度、製品形態、使 用頻度などによって適宜選択され、特に限定されるのもではない。細胞賦活化作用 を損なわない範囲で配合濃度を選択すればよぐ通常は、力力ロール濃度として 0. 0 01〜； 100, 000mg/ml、好ましくは 0.；!〜 10ng/ml、より好ましくは 0. l ~ lng/ mlである。  [0192] An extract of the Asteraceae plant obtained as described above, a refined product with an increased content of power rolls, or a pure product of power rolls can be used as an activator as it is. It is preferable to combine with cosmetics, quasi-drugs, pharmaceuticals, and foods and drinks in the form of a composition. The concentration of the extract of the Asteraceae plant is appropriately selected depending on the degree of absorption, degree of action, product form, frequency of use, etc., and is not particularly limited. It is sufficient to select the blending concentration within a range that does not impair the cell activation effect. Usually, the force roll concentration is from 0.001 to 100,000 mg / ml, preferably from 0.00 to 10 ng / ml, more preferably Is 0. l to lng / ml.
[0193] 次に、本発明の第 3および第 4の発明を実施するための最良の形態について説明す る。本発明の第 3の発明は〔1〕力力ロールを含む植物抽出物の製造方法であり、本 発明の第 4の発明は〔2〕コラーゲン産生促進剤である。  Next, the best mode for carrying out the third and fourth inventions of the present invention will be described. A third invention of the present invention is [1] a method for producing a plant extract containing a power roll, and a fourth invention of the present invention is [2] a collagen production promoter.
[0194] 本発明の第 3の発明を実施するための最良の形態について説明する。  [0194] The best mode for carrying out the third invention of the present invention will be described.
[0195] 〔1〕力力ロールを含む植物抽出物の製造方法  [0195] [1] Method for producing plant extract containing powerful roll
本発明の第 3の発明に係る力力ロールを含む植物抽出物の製造方法は、力カロー ルを含む植物抽出物から水および親水性有機溶媒の混合物を用いて二次抽出物を 得る工程を包含するものであればよぐ上記工程に供される力力ロールを含む植物抽 出物に含まれるクロロフィル含量と比較して、上記工程実施後に得られた二次抽出 物のクロロフィル含量が減少していればよい。なお、説明の便宜上、上記「力力ロール を含む植物抽出物から水および親水性有機溶媒の混合物を用いて二次抽出物を得 る工程」を、以下「工程 1」と称する。 The method for producing a plant extract containing a power roll according to the third invention of the present invention comprises a step of obtaining a secondary extract from a plant extract containing a power carol using a mixture of water and a hydrophilic organic solvent. A plant extract containing a power roll used in the above process is acceptable. It is only necessary that the chlorophyll content of the secondary extract obtained after the implementation of the above process is reduced as compared with the chlorophyll content contained in the extract. For convenience of explanation, the above “step for obtaining a secondary extract from a plant extract containing a power roll using a mixture of water and a hydrophilic organic solvent” is hereinafter referred to as “step 1”.
[0196] ここで、力力ロールについて説明する。 [0196] Here, the power roll will be described.
[0197] 力力ロールは、モミジガサの抽出物の主成分であることが知られており、式（I)で表 される構造を有している。  [0197] The power roll is known to be the main component of the extract of Momijigasa and has a structure represented by the formula (I).
[0198] [化 3] [0198] [Chemical 3]
Figure imgf000042_0001
Figure imgf000042_0001
[0199] モミジガサは、キク科コゥモリソゥ属の植物であり、 日本国内に広く分布して山菜とし て食されている。力力ロールはモミジガサ以外のキク科植物にも含まれていることが知 られており、例えば、 Cacalia [0199] Momijigasa is a plant belonging to the genus Komorisou, and is widely distributed in Japan and eaten as a wild vegetable. It is known that power rolls are also contained in asteraceae plants other than Momigasa, for example, Cacalia
adenostyloides、 Cacalia auriculata、 Ligularia virgaureaに含 れ飞いることカ幸告 れ ている。また、これら以外の植物（例えば、 Psacalium  It is reported to be included in adenostyloides, Cacalia auriculata, Ligularia virgaurea. In addition, plants other than these (for example, Psacalium
radulifolium)にも含まれて!/、ることが報告されて!/、る。  radulifolium)! /, reported to be! /
[0200] 工程 1に供される「力力ロールを含む植物抽出物」は、植物抽出物であって、力カロ ールを含むものであれば限定されない。具体的には、例えば、力力ロールを含む植 物からの一次抽出物が挙げられる。植物からの一次抽出物には通常多量のクロロフ ィルが含まれているので、これを工程 1に供することによりクロロフィル含量を減少させ ることができ、力力ロール含率の高い二次抽出物を得ることができる。 [0200] The "plant extract containing a power roll" used in step 1 is a plant extract, As long as it contains a tool. Specifically, the primary extract from the plant containing a power roll is mentioned, for example. The primary extract from plants usually contains a large amount of chlorophyll, so it can be reduced to chlorophyll content by applying it to Step 1, and the secondary extract with high power roll content. Can be obtained.
[0201] また、植物からの一次抽出物を工程 1に供して得られた二次抽出物を、「力カロー ルを含む植物抽出物」として再度工程 1に供してもよ!/、。これにより得られる二度目の 二次抽出物は、一度目の二次抽出物よりクロロフィル含量が減少している。このように 、工程 1で得られる二次抽出物中のクロロフィル含量が目的の数値以下になるまでェ 程 1を繰り返すことが好ましい。  [0201] Alternatively, the secondary extract obtained by subjecting the primary extract from the plant to Step 1 may be subjected to Step 1 again as a "plant extract containing a force carol"! /. The second secondary extract thus obtained has a lower chlorophyll content than the first secondary extract. Thus, it is preferable to repeat step 1 until the chlorophyll content in the secondary extract obtained in step 1 is less than the target value.
[0202] クロロフィル含量の目的の数値は、最終的に得られる力力ロールを含む植物抽出物 の利用目的に応じて適宜設定すればよい。力力ロールを含む植物抽出物において はクロロフィル含量を可能な限り減少させることが好ましぐ最も好ましくは、公知のク ロロフィル濃度測定法を用いて測定した場合に検出限界以下となることである。した がって、例えば、クロロフィル含量の目的の数値を検出限界値に設定すればよい。植 物抽出物中のクロロフィル濃度は、例えば、植物中抽出物を適当な溶媒に溶解し、 その吸光度を測定することにより定量できる。  [0202] The target value of the chlorophyll content may be appropriately set according to the purpose of use of the plant extract containing the power roll finally obtained. In plant extracts containing force rolls, it is preferable to reduce the chlorophyll content as much as possible. Most preferably, it is below the detection limit when measured using a known chlorophyll concentration measurement method. Therefore, for example, the target numerical value of the chlorophyll content may be set as the detection limit value. The chlorophyll concentration in the plant extract can be quantified by, for example, dissolving the plant extract in a suitable solvent and measuring the absorbance.
[0203] 以下、力力ロールを含む植物から一次抽出物を得る方法について説明する。  [0203] Hereinafter, a method for obtaining a primary extract from a plant containing a power roll will be described.
[0204] 力力ロールを含む植物から一次抽出物を得る方法は、特に限定されず、公知の方 法を適宜選択して用いることができる。  [0204] A method for obtaining a primary extract from a plant containing a power roll is not particularly limited, and a known method can be appropriately selected and used.
[0205] まず、原料とする植物の部分は、植物体全体としてもよぐ植物体を大きぐ根茎、 茎、葉と分別してそれぞれの部分を原料としてもよい。また、それぞれの部分を適宜 組み合わせたものを原料としてもよい。本発明者らは、モミジガサを原料とする場合、 根、茎、葉のうち特に葉に有効成分が多く存在することを見出しているので、葉を原 料として抽出物を得ることが好ましい。  [0205] First, plant parts as raw materials may be separated from rhizomes, stems, and leaves that are larger than the whole plant body, and the respective parts may be used as raw materials. Further, a material obtained by appropriately combining the respective parts may be used as a raw material. Since the present inventors have found that there are many active ingredients in roots, stems, and leaves, particularly leaves, when using Momijigasa as a raw material, it is preferable to obtain an extract using leaves as a raw material.
[0206] 原料の植物体は、生の状態、凍結された状態、凍結乾燥された状態のいずれを用 いてもよい。好ましくは、生の状態である。生または凍結された植物体から一次抽出 物を調製する場合には、まず、植物体を破砕してもよい。植物体の破砕にはミキサー 、ホモジナイザー、乳鉢などを用いることができる。凍結乾燥されたものは、凍結乾燥 操作後、粉状に破砕してもよい。 [0206] The raw material plant may be in a raw state, a frozen state, or a lyophilized state. Preferably, it is in a raw state. When preparing a primary extract from a raw or frozen plant body, the plant body may be first crushed. A mixer, a homogenizer, a mortar, etc. can be used for crushing a plant body. If freeze-dried, freeze-dry After the operation, the powder may be crushed.
[0207] 植物体から一次抽出物を得る方法として、溶媒を用いる方法を挙げることができる。  [0207] Examples of a method for obtaining a primary extract from a plant include a method using a solvent.
溶媒を用いる抽出方法としては、室温、冷却または加熱した状態で含浸させて抽出 する方法、水蒸気蒸留などの蒸留法を用いて抽出する方法、圧搾して抽出物を得る 圧搾法、超臨界流体または亜臨界流体を用いて抽出する方法などを好適に用いるこ とができ、これらの方法を単独で、または 2種以上を組み合わせて抽出を行うことがで きる。好ましくは、破砕した原料 lgに対して、 5〜； 100mlの溶媒を用い、 1時間から 1 力、月間、好ましくは 1〜5日間、室温あるいは加熱下で抽出を行う。  The extraction method using a solvent includes a method of extraction by impregnation in a cooled or heated state at room temperature, a method of extraction using a distillation method such as steam distillation, a compression method of obtaining an extract by pressing, a supercritical fluid or Extraction methods using subcritical fluids can be suitably used, and these methods can be used alone or in combination of two or more. Preferably, 5 to 100 ml of solvent is used for the crushed raw material lg, and extraction is performed at room temperature or under heating for 1 hour to 1 force for 1 month to 1 month, preferably 1 to 5 days.
[0208] 抽出に用いる溶媒は、有効成分である力力ロールが効果的に抽出される溶媒であ れば特に限定されるものではないが、水、またはエタノール等の低級アルコールのよ うに水と混和する有機溶媒、またはそれらの混液、または酢酸ェチル等の水と混和し ない有機溶媒が挙げられる。具体的には、例えば、メタノール，イソプロパノール，ィ ソブタノ一ノレ， n へキサノーノレ，メチノレアミノレアノレコーノレ， 2—ェチルブタノ一ノレ， n ーォクチルアルコール等の 1価アルコール類；グリセリン，エチレングリコール，ェチレ ングリコーノレモノメチノレエーテノレ，プロピレングリコーノレ，プロピレングリコーノレモノメチ ノレエーテノレ，プロピレングリコーノレモノェチノレエーテノレ， トリエチレングリコーノレ， 1 , 3 ブチレングリコ一ノレ，へキシレングリコール等の多価アルコールまたはその誘導体； アセトン，メチルェチルケトン，メチルイソブチルケトン，メチルー n プロピルケトン等 のケトン類；酢酸イソプロピル等のエステル類；ェチルエーテル，イソプロピルエーテ ノレ， n ブチルエーテル等のエーテル類；スクヮラン， ワセリン，パラフィンワックス，パ ラフィン油などの炭化水素類;ォリーブ油，小麦胚芽油，米油，ゴマ油，マ力ダミアン ナッツ油，アルモンド油，ヤシ油等の植物油脂；牛脂，豚脂，鯨油等の動物油脂など が例示される。また、リン酸緩衝生理食塩水等の無機塩類を添加した極性溶媒、界 面活性剤を添加した溶媒を用いることもでき、特に限定されない。飲食物等への使用 、皮膚への塗布を含む人体への適用における安全性等を考えれば、特に、水および /またはアルコールの!/、ずれかにより抽出することが好まし!/、。  [0208] The solvent used in the extraction is not particularly limited as long as it is a solvent from which the power roll, which is an active ingredient, can be effectively extracted, but water or water such as ethanol or a lower alcohol. Examples thereof include organic solvents that are miscible, or mixtures thereof, or organic solvents that are immiscible with water, such as ethyl acetate. Specifically, for example, monohydric alcohols such as methanol, isopropanol, isobutanol monole, n-hexanol, methenoleaminoleanoreconole, 2-ethylbutanol, n-octyl alcohol; glycerin, ethylene glycol , Ethylene glycol monomethino ethenore, propylene glycol nore, propylene glycol eno mono ethino enore, propylene glycol eno mono chineno ethenore, triethylene glycol nore, 1,3 butylene glycol mononore, hexylene glycol, etc. Polyhydric alcohol or its derivatives; Ketones such as acetone, methyl ethyl ketone, methyl isobutyl ketone, methyl n propyl ketone; Esters such as isopropyl acetate; Ethyl ether, Isopropyl ether, n Butyl ether Ethers such as oil; hydrocarbons such as squalene, petrolatum, paraffin wax, paraffin oil; vegetable oils such as olive oil, wheat germ oil, rice oil, sesame oil, mackerel damian nut oil, almond oil, coconut oil; Examples include animal fats such as beef tallow, pork tallow and whale oil. In addition, a polar solvent to which inorganic salts such as phosphate buffered saline are added and a solvent to which a surfactant is added can be used without any particular limitation. Considering safety for use in food and drink, application to the human body including application to the skin, etc., it is particularly preferable to extract with water and / or alcohol! /.
[0209] また、段階的に抽出操作を行ってもよ!/、。例えば、エタノール等の低級アルコール のように水と混和する有機溶媒で抽出操作を行い、エバポレーターなどで濃縮し、酢 酸ェチル等の水と混和しない有機溶媒と水とを加え、有機溶媒層と水層に分離して あよい。 [0209] The extraction operation may be performed step by step! /. For example, extraction is performed with an organic solvent that is miscible with water, such as a lower alcohol such as ethanol, and the mixture is concentrated with an evaporator or the like. An organic solvent immiscible with water, such as oxyethyl, and water may be added and separated into an organic solvent layer and an aqueous layer.
[0210] このようにして得られた一次抽出物は、溶媒を除去することによって濃縮乾固物と することが好ましい。  [0210] The primary extract thus obtained is preferably concentrated to dryness by removing the solvent.
[0211] 次に、工程 1について説明する。 [0211] Next, step 1 will be described.
[0212] 工程 1では、例えば上述のようにして得られた力力ロールを含む植物からの一次抽 出物に対して、水および親水性有機溶媒の混合物を用いて二次抽出物を得る。なお 、上述のように、工程 1に供される力力ロールを含む植物抽出物は、植物からの一次 抽出物に限定されず、既に工程 1の抽出処理を施した二次抽出物も対象として好適 である。  [0212] In Step 1, for example, a secondary extract is obtained using a mixture of water and a hydrophilic organic solvent with respect to a primary extract from a plant containing a power roll obtained as described above. As described above, the plant extract containing the power roll used in step 1 is not limited to the primary extract from the plant, but also includes the secondary extract that has already been subjected to the extraction process in step 1. Is preferred.
[0213] 親水性有機溶媒としては、例えば、メタノール、エタノール、プロピルアルコール、ィ ソプロピルアルコール、ブタノール等の炭素数 1〜5の低級アルコール；アセトン、メチ ルェチルケトン等の低級脂肪族ケトン； 1 , 3ーブチレングリコール、プロピレングリコ ール、グリセリン等の炭素数 2〜5の多価アルコールなどが挙げられ、これらは 1種単 独で使用してもよいし、 2種以上併用して使用してもよい。なお、一次抽出に用いる溶 媒と同様に、二次抽出に用いる親水性有機溶媒についても、人体への適用における 安全性等を考慮して、特に、エタノール、アセトン、イソプロピルアルコール、プロピレ ングリコール、ブチレングリコール、ブタノールを用いることが好ましい。 [0213] Examples of the hydrophilic organic solvent, such as methanol, ethanol, propyl alcohol, I isopropyl alcohol, lower alcohols having 1 to 5 carbon atoms in butanol; acetone, lower aliphatic ketones such as methylcarbamoyl Ruechiruketon; 1, 3 Examples include polyhydric alcohols having 2 to 5 carbon atoms such as butylene glycol, propylene glycol, and glycerin. These may be used alone or in combination of two or more. Good. As with the solvent used for the primary extraction, the hydrophilic organic solvent used for the secondary extraction is particularly considered ethanol, acetone, isopropyl alcohol, propylene glycol, It is preferable to use butylene glycol and butanol.
[0214] 水および親水性有機溶媒の混合物における好まし!/、含水率、すなわち、二次抽出 物中のクロロフィル含量が工程 1に供した力力ロールを含む植物抽出物中のクロロフ ィル含量より減少するようになる水および親水性有機溶媒の混合物の含水率は、親 水性有機溶媒の種類、原料植物の種類、原料植物体の使用部分等によって異なる ので、その都度最適な含水率を予備的に確認することが好ましい。  [0214] Preferable in a mixture of water and hydrophilic organic solvent! /, Moisture content, ie chlorophyll content in the plant extract containing the power roll that the chlorophyll content in the secondary extract was subjected to step 1 The water content of the mixture of water and hydrophilic organic solvent, which will be further reduced, varies depending on the type of hydrophilic organic solvent, the type of raw material plant, the part of the raw material plant used, etc. It is preferable to confirm it.
[0215] 例えば、親水性溶媒にエタノールを用いた場合、エタノール含量が 45%以上の含 水エタノールを用いることが好ましぐより好ましくはエタノール含量が 50%〜70%の 含水エタノール、さらに好ましくはエタノール含量が 55%〜65%の含水エタノールで ある。 [0215] For example, when ethanol is used as the hydrophilic solvent, it is preferable to use hydrous ethanol having an ethanol content of 45% or more, more preferably hydrous ethanol having an ethanol content of 50% to 70%, more preferably Hydrous ethanol with an ethanol content of 55% to 65%.
[0216] 第 1工程は、例えば、第 1工程に供される力力ロールを含む植物抽出物に水および 親水性有機溶媒の混合物を加えて静置後、上清を回収することにより実施できる。 [0216] The first step is, for example, adding water and It can be carried out by adding a mixture of hydrophilic organic solvents and allowing to stand, and then collecting the supernatant.
[0217] 工程 1を行うことにより、力力ロールの含有率が高くクロロフィルの含有率が低い植 物抽出物を効率よぐ低コストで得ること力 Sできる。また、工程 1を繰り返すことにより、 クロロフィルをほとんど含有しない力力ロールを含む植物抽出物を得ることができる。 なお、植物抽出物中のクロロフィル濃度は、例えば、植物中抽出物を適当な溶媒に 溶解し、その吸光度を測定することにより定量できる。 [0217] By performing Step 1, it is possible to obtain a plant extract having a high content of power rolls and a low content of chlorophyll at a low cost in terms of efficiency. Further, by repeating Step 1, a plant extract containing a power roll containing almost no chlorophyll can be obtained. The chlorophyll concentration in the plant extract can be quantified by, for example, dissolving the plant extract in a suitable solvent and measuring the absorbance.
[0218] また、さらに得られた力力ロールを含む抽出物中の有効成分の濃度を高め、完全に クロロフィルを除去するときは、所望により、得られた抽出物をさらに、濃縮、液液分配 、吸着クロマトグラフィー、順相もしくは逆相クロマトグラフィー等の手段に付すことも 可能である。 [0218] Further, when the concentration of the active ingredient in the extract containing the force roll obtained is further increased and chlorophyll is completely removed, the obtained extract is further concentrated and liquid-liquid distributed as desired. It can also be subjected to means such as adsorption chromatography, normal phase or reverse phase chromatography.
[0219] 本発明の第 3の発明に係る力力ロールを含む植物抽出物の製造方法は、工程 1 (力 力ロールを含む植物抽出物から水および親水性有機溶媒の混合物を用いて二次抽 出物を得る工程）に加えて、さらに、力力ロールを含む植物抽出物から、水、または、 水および親水性有機溶媒の混合物を用いて力力ロールより高親水性の夾雑物を除 去する工程を包含することが好ましい。この工程を加えることにより、クロロフィル含量 および高親水性夾雑物含量がいずれも低い力力ロールを含む植物抽出物を得ること 力できる。高親水性夾雑物含量が低いと力力ロール純度が高くなり、安定したプレミツ タス品を調製できるという利点がある。なお、説明の便宜上、上記「力力ロールを含む 植物抽出物から、水、または、水および親水性有機溶媒の混合物を用いて力カロー ルより高親水性の夾雑物を除去する工程」を、以下「工程 2」と称する。  [0219] A method for producing a plant extract containing a power roll according to the third invention of the present invention comprises a step 1 (secondary extraction from a plant extract containing a power roll using a mixture of water and a hydrophilic organic solvent. In addition to the step of obtaining the extract), contaminants that are more hydrophilic than the power roll are removed from the plant extract containing the power roll using water or a mixture of water and a hydrophilic organic solvent. It is preferable to include a step of leaving. By adding this step, it is possible to obtain a plant extract containing a power roll in which both the chlorophyll content and the highly hydrophilic contaminant content are low. When the content of highly hydrophilic contaminants is low, the strength of the power roll is increased, and there is an advantage that a stable premitus product can be prepared. For convenience of explanation, the above-mentioned “step of removing contaminants having a higher hydrophilicity than the power color from water or a mixture of water and a hydrophilic organic solvent from the plant extract containing the power roll” Hereinafter referred to as “Step 2”.
[0220] 工程 2に供される「力力ロールを含む植物抽出物」としては、既に説明した力カロー ルを含む植物から得た一時抽出物、既に工程 1の抽出処理を施した二次抽出物、さ らに工程 1の抽出処理を繰り返し施した抽出物を挙げることができる。なお、工程 2で は、得られた抽出物中に高親水性の夾雑物が含まれるので、これを除いた残渣を回 収することになる。したがって、工程 2に供される「力力ロールを含む植物抽出物」は 溶媒を除去した濃縮乾固物であることが好ましい。  [0220] The “plant extract containing a power roll” used in Step 2 is a temporary extract obtained from a plant containing a power carol described above, or a secondary extraction that has already been subjected to the extraction process of Step 1. And an extract obtained by repeatedly performing the extraction process in Step 1. In step 2, since highly hydrophilic impurities are contained in the obtained extract, the residue excluding this is collected. Therefore, it is preferable that the “plant extract containing a power roll” to be used in step 2 is a concentrated dry product from which the solvent has been removed.
[0221] 工程 2は工程 1の前段に設けてもよぐ工程 1の後段に設けてもよい。また、工程 1を 繰り返す場合には、先の工程 1と後の工程 1の間に工程 2を設けてもよい。工程 2では クロロフィル含量を減少させることは困難であることから、工程 1の後段に工程 2を設 けることが好ましい。 [0221] Step 2 may be provided before Step 1 or after Step 1. When step 1 is repeated, step 2 may be provided between the previous step 1 and the subsequent step 1. In process 2 Since it is difficult to reduce the chlorophyll content, it is preferable to provide step 2 after step 1.
[0222] 工程 2で好適に用いられる親水性有機溶媒は、工程 1の説明にお!/、て例示したも のと同じである。水および親水性有機溶媒の混合物における好ましい含水率、すな わち、力力ロールより高親水性の夾雑物を効率よく除去できる水および親水性有機 溶媒の混合物の含水率は、親水性有機溶媒の種類、原料植物の種類、原料植物体 の使用部分等によって異なるので、その都度最適な含水率を予備的に確認すること が好ましい。  [0222] The hydrophilic organic solvent suitably used in Step 2 is the same as that exemplified in the description of Step 1. The preferred water content in the mixture of water and hydrophilic organic solvent, that is, the water content of the mixture of water and hydrophilic organic solvent that can efficiently remove impurities that are more hydrophilic than the power roll is Therefore, it is preferable to preliminarily check the optimal water content each time.
[0223] 例えば、親水性溶媒にエタノールを用いた場合、エタノール含量が 40%以下の含 水エタノールを用いることが好ましぐより好ましくはエタノール含量が 10%〜30%の 含水エタノール、さらに好ましくはエタノール含量が 15%〜25%の含水エタノールで ある。なお、工程 2では、親水性有機溶媒を含まない水を抽出溶媒として用いること ができる。  [0223] For example, when ethanol is used as the hydrophilic solvent, it is preferable to use hydrous ethanol having an ethanol content of 40% or less, more preferably hydrous ethanol having an ethanol content of 10% to 30%, and even more preferably. It is hydrous ethanol with an ethanol content of 15% to 25%. In step 2, water containing no hydrophilic organic solvent can be used as the extraction solvent.
[0224] 第 2工程は、例えば、この工程に供される力力ロールを含む植物抽出物に水、また は、水および親水性有機溶媒の混合物を加えて静置後、沈殿物を回収することによ り実施できる。また、力力ロールより高親水性の夾雑物が除去されたことは、抽出物重 量の減少により確認することができる。  [0224] In the second step, for example, water or a mixture of water and a hydrophilic organic solvent is added to a plant extract containing a power roll used in this step, and the mixture is allowed to stand, and then the precipitate is recovered. Can be implemented. The removal of highly hydrophilic impurities from the power roll can be confirmed by the decrease in the weight of the extract.
[0225] 上記第 1工程を経て得られた力力ロールを含む植物抽出物、または、上記第 1工程 および第 2工程を経て得られた力力ロールを含む植物抽出物は、溶媒を除去すること によって乾固物とすることができ、さらに、アルコールなどの溶媒に可溶化した形態、 あるいは乳剤の形態で用いることができる。  [0225] The plant extract containing the power roll obtained through the first step or the plant extract containing the power roll obtained through the first step and the second step removes the solvent. Thus, it can be made into a dried product, and further, it can be used in a form solubilized in a solvent such as alcohol or an emulsion.
[0226] 本発明の第 3の発明に係る力力ロールを含む植物抽出物の製造方法は、力カロー ルを含む植物であればどのような植物にも適用できる力 キク科植物に適用すること が好ましい。キク科植物としては、アークトセカ属、アキノキリンソゥ属、アキノノゲシ属 、ァザミ属、  [0226] The method for producing a plant extract containing a power roll according to the third invention of the present invention is applicable to a power plant that can be applied to any plant as long as it contains a power carol. Is preferred. As for Asteraceae plants, there are Arctoseca, Akinokirinso, Akinonogeshi, Azami,
ァゼトウナ属、アフリカキンセンカ属、ァレチアザミ属、ァヮユキギク属、ィズハハコ属、 ゥサギギク属、ウスべニニガナ属、ウスユキソゥ属、ェゾギク属、ェゾコゥゾリナ属、ェ ゾノチチコダサ属、才才ァザミ属、才才センボンャリ属、才才ハンゴンソゥ属、ォォヒレ ァザミ属、才才モミジガサ属、ォグルマ属、オケラ属、ォナモミ属、ォニタビラコ属、ォ ランダセン二チ属、力イザイク属、カツコゥァザミ属、力ミツレモドキ属、ガザ二ァ属、ガ ンクビソゥ属、キオン属、キクニガナ属、キク属、キッネアザミ属、キリンギク属、キンセ ンカ属、クサャッデ属、ケントラタルム属、コゥォゥソゥ属、コゥゾリナ属、コゥモリソゥ属 、コゥャボウキ属、コケセンボンギク属、コゴメギク属、コスモス属、コレオステフス属、 ゴボウ属、ゴマギク属、サヮギク属、サンシチソゥ属、シオン属、シカギク属、シユウブ ンソゥ属、シユンギク属、ショウジヨウハグマ属、スイラン属、ステビア属、ストケシァ属、 センダンダサ属、センボンャリ属、タカサゴトキンソゥ属、タカサブロウ属、タケダグサ 属、タムラソゥ属、タンポポモドキ属、タンボポ属、ダリァ属、チョウセンァザミ属、ツル ハグマ属、ッヮブキ属、トウヒレン属、トキンソゥ属、ニガナ属、ヌマダイコン属、ノース ポールギク属、ノゲシ属、ノコギリソゥ属、ノブキ属、ハゴロモギク属、ハナカンザシ属、 ノヽナヮギク属、ノヽネミギク属、ハハコグサ属、ノヽマギク属、ハマグルマ属、ハルシャギ ク属、バラモンジン属、バルサムギク属、ヒゴタイ属、ヒナギク属、ヒマヮリ属、ヒメキクタ ビラコ属、ヒメジョオン属、ヒャクニチソゥ属、ヒョドリバナ属、ヒレアザミ属、フェリキア属 、フキタンポポ属、フキ属、フクォゥソゥ属、フタナミソゥ属、フタマタタンポポ属、フラン スギク属、ブクリヨウサイ属、ブタクサ属、へレニウム属、ベニバナポロギク属、ベニバ ナ属、ポリムニァ属、マメカミツレ Azatouna, African calendula, Aletiazami, Ayuyukigi, Izhahako, Usagigi, Usubenigana, Usukikisou, Yezogiku, Ezokouzorina, Ezonotikodosa, Talent Genus, ohile Genus genus, genus maple, genus ogurma, okera, onamomi, onitabiraco, olandasenji, genus izaiku, katsura-zami, genus genus genus, genus genus genus, genus genus, chion Genus, chrysanthemum, quince thistle, giraffe, kinsengka, kushydede, centratarum, kosou, kozolina, komorisou, kobobuki, koshsenbongi, kogomegi, kosmos , Sageki genus, Sansitoshi, Zion genus, Sikagiku genus, Syubunsou genus, Syungyoku genus, Dwarf sage bear genus, Suiran genus, Stevia genus, Stroke genus, Sendanda genus, Sembonari genus, Takasagotokin genus, Takasago genus , Ta Rasou, Dandelion, Tambopo, Daria, Datura, Crane, Beard, Spruce, Tokinsou, Nana, Nudadai, North Pole, Nogeshi, Yokogi, Nobuki, Chrysanthemum spp., Hankanzashi spp., Prunus spp., Pepper spp., Pleurotus spp., Papaver spp., Prunus spp., Harshagp, Baramondin, Balsam, Pleurotus spp., Chrysanthemum spp. Chrysanthemum genus, Hydrobana genus, Greater thistle genus, Felicia genus, Fuchsia poppo genus, Fuchsia genus, Fuchus genus, Futana genus, Futama dandelion genus, Francis genus, Buddlesia genus, Ragweed genus, Helenium genus, Safflower genus Porimunia genus, Mamekamitsure
属、ミコシギク属、 Genus, wormwood,
ミスミグサ属、ミズヒマヮリ属、ミヤマョメナ属、ムカショモギ属、ムギワラギク属、メタカラ コゥ属、メナモミ属、メランポディウム属、モクシユンギク属、モクビヤッコゥ属、モミジハ グマ属、ャグノレマギク属、ャナギタンポポ属、ャブタビラコ属、ャブレガサ属、ヤマハ ハコ属、ャマボクチ属、ユリォプス属、ユリネア属、ョモギギク属、ョモギ属、ヮダンノキ 属が挙げられるが、力力ロールを含有する植物であれば特にキク科に限るものではな いが、その中でもコゥモリソゥ属植物が好ましい。 Misumigusa, Mizuhimabori, Miyamamena, Muchasomoghi, Mugiwaragiku, Metakarakoma, Menamomi, Melanpodium, Mokuyungiku, Mokubiyakkou, Momijihaguma, Yakuno-gama, Amanagambipha Genus, wildflower genus, yuryopus genus, genus genus, mugwort genus, mugwort genus, dandelion genus, but it is not particularly limited to the family Asteraceae as long as it contains a power roll. Is preferred.
コゥモリソゥ属植物としては、コゥモリソゥ、モミジガサ、力ニコゥモリ、タマブキ、ィヌド ゥナ、ィズカニコゥモリ、ォォカニコゥモリ、タイミンガサ、ックシコゥモリソゥ、テバコモミ ジガサ、ニシノヤマタイミンガサ、ミミコゥモリ、モミジコゥモリ、ヤマタィミンガサ、ョブス マソゥなどが挙げられる。その中でも、食用植物が好ましぐモミジガサが適している。 [0228] 本発明の第 4の発明を実施するための最良の形態について説明する。 Among the genus bats are bats, maples, powers, tamabuki, inuduna, izkanikomori, okanakumori, taimingasa, kushikoumoriso, tebacomomijigasa, nishinoyamataiminga, It is done. Among them, Momijigasa, which is preferred by edible plants, is suitable. The best mode for carrying out the fourth invention of the present invention will be described.
[0229] 〔2〕コラーゲン産生促進剤 [0229] [2] Collagen production promoter
本発明の第 4の発明に係るコラーゲン産生促進剤は力力ロールを有効成分として 含有するものであればよい。力力ロールは上述の本発明の第 3の発明に係る製造方 法により力力ロールを含む植物抽出物として得ることができる。  The collagen production promoter according to the fourth invention of the present invention only needs to contain a force roll as an active ingredient. The power roll can be obtained as a plant extract containing the power roll by the production method according to the third aspect of the present invention.
[0230] 有効成分である力力ロールの含量を高めた精製品、あるいは有効成分である力カロ ール純品としても、本発明の第 4の発明に係るコラーゲン産生促進剤の有効成分とし て好適に用いることができる。 [0230] A refined product with an increased content of a power roll as an active ingredient, or a pure power carol product as an active ingredient is also used as an active ingredient of the collagen production promoter according to the fourth invention of the present invention. It can be used suitably.
[0231] また、力力ロールはタレゾールメチルエステルを原料として化学合成することができる [0231] The power roll can be chemically synthesized using talesol methyl ester as a raw material.
(Chem. Lett. , 1317 (1975)を参照）。このように化学合成により得られる力力ロールも 本発明の第 4の発明に係るコラーゲン産生促進剤の有効成分として好適に用いるこ と力 Sできる。  (See Chem. Lett., 1317 (1975)). Thus, a force roll obtained by chemical synthesis can also be suitably used as an active ingredient of the collagen production promoter according to the fourth invention of the present invention.
[0232] 上述の本発明の第 3の発明に係る製造方法により力力ロールを含む植物抽出物は 、そのままコラーゲン産生促進剤として利用することも可能である力 化粧品、医薬部 外品、医薬品、飲食品に配合して組成物の形態で実施することが好ましい。力カロー ルを含む植物抽出物を配合する濃度は、吸収程度、作用程度、製品形態、使用頻 度などによって適宜選択され、特に限定されるのもではない。コラーゲン産生促進作 用を損なわない範囲で配合濃度を選択すればよぐ通常は、力力ロール濃度として 0 . 001— 100, 000ng/ml、好ましくは 0. 01 ~ 10ng/ml,より好ましくは 0. 01— 1 ng/ mlでめる。  [0232] The plant extract containing a powerful roll by the production method according to the third aspect of the present invention described above can be used as a collagen production promoter as it is. Cosmetics, quasi drugs, pharmaceuticals, It is preferable to implement in the form of a composition by blending with food or drink. The concentration of the plant extract containing force carol is appropriately selected depending on the degree of absorption, degree of action, product form, frequency of use, etc., and is not particularly limited. It is sufficient to select the blending concentration within the range that does not impair the collagen production promoting action. Normally, the force roll concentration is 0.001 to 100,000 ng / ml, preferably 0.01 to 10 ng / ml, more preferably 0. 01—Use 1 ng / ml.
[0233] 本発明の第 2の発明に係る賦活化剤、および本発明の第 4の発明に係るコラーゲン 産生促進剤は、化粧品、医薬部外品、医薬品、飲食品に配合して組成物の形態で 実施することが好ましレ、。以下のその形態にっレ、て説明する。  [0233] The activator according to the second invention of the present invention and the collagen production promoter according to the fourth invention of the present invention are formulated into cosmetics, quasi-drugs, pharmaceuticals, and foods and drinks. It is preferable to carry it out in the form. The following forms will be described.
[0234] 化粧品、医薬部外品、医薬品の形態で実施する場合、外用剤とすることが好適で ある。また、食用とされているキク科植物の抽出物は経口摂取も可能であるため、外 用剤に限定されず、内用剤、飲食品としてもよい。 [0234] When implemented in the form of cosmetics, quasi-drugs, and pharmaceuticals, it is preferable to use external preparations. In addition, the extracts of the Asteraceae plants that are considered to be edible can be taken orally, so they are not limited to external preparations but may be internal preparations and foods and drinks.
[0235] 剤形は限定されず、アンプル、カプセル、粉末、顆粒、丸剤、錠剤、固形剤、液剤、 ゲル、気泡、乳液、クリーム、軟膏、シート、ムース、浴用剤など多様なものとすること ができる。 [0235] The dosage form is not limited, and ampules, capsules, powders, granules, pills, tablets, solids, liquids, gels, bubbles, emulsions, creams, ointments, sheets, mousses, bath preparations, etc. thing Can do.
[0236] 具体的には、化粧品、医薬部外品、医薬品としては、例えば内用 ·外用薬用製剤、 化粧水、乳液、クリーム、軟膏、ローション、オイル、パックなどの基礎化粧料、洗顔料 や皮膚洗浄料、シャンプー、リンス、ヘアートリートメント、ヘアクリーム、ポマード、へ ァスプレー、整髪料、パーマ剤、ヘアートニック、染毛料、育毛 '養毛料などの頭髪化 粧料、ファンデーション、白粉、おしろい、口紅、頰紅、アイシャドウ、アイライナー、マ スカラ、眉墨、まつ毛などのメークアップ化粧料、美爪料などの仕上げ用化粧料、香 水類、浴用剤、その他、歯磨き類、口中清涼剤 ·含嗽剤、液臭 ·防臭防止剤、衛生用 品、衛生綿類、ウエットティシュなどが挙げられる。  [0236] Specifically, cosmetics, quasi-drugs, and pharmaceuticals include, for example, internal and external pharmaceutical preparations, skin lotions, emulsions, creams, ointments, lotions, oils, packs, and other basic cosmetics, face wash, Skin cleansers, shampoos, rinses, hair treatments, hair creams, pomades, hair sprays, hair styling agents, permanents, hair nicks, hair dyes, hair restorations such as hair conditioners, cosmetics, foundations, white powder, funny hair, lipstick, Makeup cosmetics such as scarlet, eye shadow, eyeliner, mascara, eyebrows, eyelashes, cosmetics for finishing such as nail polish, perfumes, bath preparations, other toothpastes, mouth fresheners and mouthwashes Liquid odor / deodorant, hygiene products, sanitary cotton, wet tissue, etc.
[0237] 具体的には、飲食品としては、例えば清涼飲料、炭酸飲料、栄養飲料、果実飲料、 乳酸飲料等の飲料、アイスクリーム、アイスシャーベット、力、き氷等の冷菓、そば、うど ん、はるさめ、ぎょうざの皮、しゅうまいの皮、中華麵、即席麵等の麵類、飴、キャンデ ィー、ガム、チョコレート、錠菓、スナック菓子、ビスケット、ゼリー、ジャム、クリーム、焼 き菓子、パン等の菓子類、力二、サケ、アサリ、マグロ、イワシ、ェビ、カツォ、サノ 、ク ジラ、カキ、サンマ、イカ、ァカガイ、ホタテ、ァヮビ、ゥュ、イクラ、トコブシ等の水産物 、かまぼこ、ハム、ソーセージ等の水産'畜産加工食品、加工乳、発酵乳等の乳製品 、サラダ油、てんぷら油、マーガリン、マヨネーズ、ショートニング、ホイップクリーム、ド レッシング等の油脂および油脂加工食品、ソース、たれ等の調味料、カレー、シチュ 一、親子丼、お粥、雑炊、中華丼、かつ丼、天丼、うな丼、ノ、ヤシライス、おでん、マ ーボドーフ、牛丼、ミートソース、玉子スープ、オムライス、餃子、シユーマイ、ハンバ ーグ、ミートボール等のレトルトバウチ食品、種々の形態の健康 ·栄養補助食品、保 健機能食品、錠剤、カプセル剤、ドリンク剤、トローチなどが挙げられる。  [0237] Specifically, examples of food and drink include soft drinks, carbonated drinks, nutrition drinks, fruit drinks, lactic acid drinks, ice cream, ice sherbet, power, frozen ice confectionery such as soba, udon , Harusame, Gyoza skin, Pomegranate skin, Chinese rice cake, instant rice cake, etc., rice cake, candy, gum, chocolate, tablet confectionery, snack confectionery, biscuit, jelly, jam, cream, baked confectionery, bread, etc. Confectionery, wisteria, salmon, clams, tuna, sardines, shrimp, bonito, sano, whales, oysters, saury, squid, squid, scallops, scallops, abalone, yu, ichura, tokobushi and other seafood, kamaboko, ham , Fishery products such as sausages, livestock processed foods, processed milk, dairy products such as fermented milk, salad oil, tempura oil, margarine, mayonnaise, shortening, whipped cream, dressin Oil and fat processed foods, seasonings such as sauces, sauces, curry, stew, oyakodon, rice cakes, miscellaneous cooking, Chinese rice cakes, and rice cakes, tempura, eel rice, rice, rice rice, oden, mabodorf, gyudon , Meat sauces, egg soup, omelet rice, dumplings, yumai, hamburg, meatballs and other retort bouch foods, various forms of health and nutritional supplements, health functional foods, tablets, capsules, drinks, troches, etc. Can be mentioned.
[0238] 本発明の第 2の発明に係る賦活化剤または本発明の第 4の発明に係るコラーゲン 産生促進剤はヒトに対して好適に適用されるものである力 それぞれの作用効果が 期待できる限り、ヒト以外の動物に対して適用することもできる。  [0238] The activator according to the second invention of the present invention or the collagen production promoter according to the fourth invention of the present invention can be suitably applied to humans. As long as it is applicable to animals other than humans.
[0239] 本発明の第 2の発明に係る賦活化剤または本発明の第 4の発明に係るコラーゲン 産生促進剤は、有効成分であるキク科植物の抽出物に加え、必要に応じて本発明の 第 2または第 4の発明の効果を損なわない範囲内で、化粧品、医薬部外品、医薬品 、飲食品に使用される成分や添加剤を併用して配合することができる。 [0239] The activator according to the second invention of the present invention or the collagen production promoter according to the fourth invention of the present invention is added to the asteraceae plant extract, which is an active ingredient, as required. As long as the effects of the second or fourth invention are not impaired, cosmetics, quasi drugs and pharmaceuticals The ingredients and additives used in food and drink can be used in combination.
[0240] 例えば、下記に示すような化粧品類などで通常使用される基剤、添加剤など、例え ば油脂類、ロウ類、鉱物油、脂肪酸類、アルコール類、エステル類、金属セッケン、ガ ム質および水溶性高分子化合物、界面活性剤、ビタミン類、アミノ酸、美白剤、保湿 剤、微生物培養代謝産物、 ヒドロキシ酸、無機顔料、紫外線吸収剤、収斂剤、抗 菌剤、殺菌，消毒薬、頭髪用剤、香料、色素 ·着色剤、甘味料、栄養強化剤、育毛剤 、動植物抽出物、抗酸化剤、抗炎症剤等と併用して用いることができる。  [0240] For example, bases and additives usually used in cosmetics as shown below, such as fats and oils, waxes, mineral oils, fatty acids, alcohols, esters, metal soap, gum Quality and water-soluble polymer compounds, surfactants, vitamins, amino acids, whitening agents, moisturizers, microbial culture metabolites, hydroxy acids, inorganic pigments, UV absorbers, astringents, antibacterial agents, bactericides, disinfectants, It can be used in combination with hair preparations, fragrances, pigments / colorants, sweeteners, nutrition enhancers, hair restorers, animal and plant extracts, antioxidants, anti-inflammatory agents, and the like.
[0241] 例えば、油脂類としては、アポガド油、アルモンド油、ウイキヨゥ油、エゴマ油、オリー ブ油、オレンジ油、オレンジラファー油、ゴマ油、カカ才 β旨、力ミツレ油、力ロット油、キ ユーカンバー油、牛脂、牛脂脂肪酸、ククイナッツ油、サフラワー油、大豆油、ツバキ 油、トウモロコシ油、ナタネ油、パーシック油、ヒマシ油、綿実油、落花生油、タートル 油、ミンク油、卵黄油、カカオ脂、パーム油、パーム核油、モクロウ、ヤシ油、牛脂、豚 脂、硬化油、硬化ヒマシ油などが挙げられる。  [0241] For example, as fats and oils, apogad oil, almond oil, wikiyo oil, sesame oil, olive oil, orange oil, orange rafa oil, sesame oil, kaka-age β, power mitsule oil, power lot oil, cucumber Oil, beef tallow, beef tallow fatty acid, cucumber nut oil, safflower oil, soybean oil, camellia oil, corn oil, rapeseed oil, persic oil, castor oil, cottonseed oil, peanut oil, turtle oil, mink oil, egg yolk oil, cocoa butter, palm Oil, palm kernel oil, owl, coconut oil, beef tallow, lard, hydrogenated oil, hydrogenated castor oil.
[0242] 例えば、ロウ類としては、ミツロウ、カルナバロウ、鯨ロウ、ラノリン、液状ラノリン、還 元ラノリン、硬質ラノリン、カンデリラロゥ、モンタンロウ、セラックロウなどが挙げられる。  [0242] For example, waxes include beeswax, carnauba wax, whale wax, lanolin, liquid lanolin, reduced lanolin, hard lanolin, candelillaro, montan wax, shellac wax and the like.
[0243] 例えば、鉱物油としては、流動パラフィン、ワセリン、パラフィン、ォゾケライド、セレシ ン、マイクロクリスタンワックス、ポリエチレン末、スクワレン、スクヮラン、プリスタンなど が挙げられる。  [0243] Examples of mineral oils include liquid paraffin, petrolatum, paraffin, ozokelide, ceresin, microcrystalline wax, polyethylene powder, squalene, squalane, and pristane.
[0244] 例えば、脂肪酸類としては、ラウリン酸、ミリスチン酸、ノ ルミチン酸、ステアリン酸、 ベヘン酸、ォレイン酸、 12-ヒドロキシステアリン酸、ゥンデシレン酸、トール油、 ラノリン脂肪酸などの天然脂肪酸、イソノナン酸、カプロン酸、 2—ェチルブタン酸、ィ ソペンタン酸、 2—メチルペンタン酸、 2—ェチルへキサン酸、イソペンタン酸などの合 成脂肪酸が挙げられる。  [0244] For example, as fatty acids, natural fatty acids such as lauric acid, myristic acid, normitic acid, stearic acid, behenic acid, oleic acid, 12-hydroxystearic acid, undecylenic acid, tall oil, lanolin fatty acid, and isononanoic acid And synthetic fatty acids such as caproic acid, 2-ethylbutanoic acid, isopentanoic acid, 2-methylpentanoic acid, 2-ethylhexanoic acid and isopentanoic acid.
[0245] 例えば、アルコール類としては、エタノール、イソプロパノール、ラウリルアルコール 、セタノーノレ、ステアリノレアノレコーノレ、ォレイノレアノレコーノレ、ラノリンァノレコーノレ、 コレス テロール、フィトステロールなどの天然アルコーノレ、 2—へキシルデカノーノレ、イソステ ァリルアルコール、 2—オタチルドデカノールなどの合成アルコーノレ、酸化エチレン、 エチレングリコーノレ、ジエチレングリコーノレ、トリエチレングリコーノレ、エチレングリコー ノレモノェチノレエーテノレ、エチレングリコーノレモノブチノレエーテノレ、ジエチレングリコー ノレモノメチノレエーテノレ、ジエチレングリコ一ノレモノェチノレエーテノレ、ポリエチレングリコ 一ノレ、酸化プロピレン、プロピレングリコーノレ、ポリプロピレングリコール、 1 , 3—ブチ レングリコーノレ、グリセリン、ノ チノレアノレコーノレ、ペンタエリトリトーノレ、ソノレビトーノレ、マ ンニトール、ブドウ糖、ショ糖などの多価アルコール類などが挙げられる。 [0245] For example, the alcohols include natural alcohols such as ethanol, isopropanol, lauryl alcohol, cetanol, stearinoreanoleconore, oleinoleanoreconole, lanolinanoreconole, cholesterol, phytosterol, etc. Synthetic alcohols such as xyldecanol, isostearyl alcohol, 2-octyldodecanol, ethylene oxide, ethylene glycol, diethylene glycol, triethylene glycol, ethylene glycol Noremonochinenoatenore, ethyleneglycolenobutinorenoatenore, diethyleneglycol noremonomethinoreatenore, diethyleneglycolenolemonotinoreatenore, polyethyleneglycolinole, propyleneoxide, propyleneglycolole, polypropyleneglycol 1, 3-butyleneglycolone, glycerin, notinorenoreconole, pentaerythritole, sonorebitonore, mannitol, glucose, sucrose and other polyhydric alcohols.
[0246] 例えば、エステル類としては、ミリスチン酸イソプロピル、パルミチン酸イソプロピル、 ステアリン酸プチル、ラウリン酸へキシル、ミリスチン酸ミリスチル、ォレイン酸ォレイル 、ォレイン酸デシル、ミリスチン酸オタチルドデシル、ジメチルオクタン酸へキシルデシ ノレ、乳酸セチル、乳酸ミリスチル、フタル酸ジェチル、フタル酸ジブチル、酢酸ラノリン 、モノステアリン酸エチレングリコール、モノステアリン酸プロピレングリコール、ジォレ イン酸プロピレングリコールなどが挙げられる。 [0246] For example, as esters, isopropyl myristate, isopropyl palmitate, butyl stearate, hexyl laurate, myristyl myristate, oleyl oleate, decyl oleate, octyldodecyl myristate, hexyl decyl octanoate Nole, cetyl lactate, myristyl lactate, jetyl phthalate, dibutyl phthalate, lanolin acetate, ethylene glycol monostearate, propylene glycol monostearate, propylene glycol dioleate and the like.
[0247] 例えば、金属セッケンとしては、ステアリン酸アルミニウム、ステアリン酸マグネシウム 、ステアリン酸亜鉛、ステアリン酸カルシウム、パルミチン酸亜鉛、ミリスチン酸マグネ シゥム、ラウリン酸亜鉛、ゥンデシレン酸亜鉛などが挙げられる。  [0247] Examples of the metal soap include aluminum stearate, magnesium stearate, zinc stearate, calcium stearate, zinc palmitate, magnesium myristate, zinc laurate, zinc undecylenate and the like.
[0248] 例えば、ガム質および水溶性高分子化合物としては、アラビアゴム、ベンゾインゴム 、ダンマルゴム、グアヤタ脂、アイルランド苔、カラャゴム、トラガントゴム、キヤロブゴム 、クインシード、寒天、カゼイン、デキストリン、ゼラチン、ぺクチン、デンプン、カラギー ン、キトサン塩、硫酸化キチン、リン酸化キチン、アルギン酸およびその塩、ヒアルロン 酸およびその塩、コンドロイチン硫酸、へパリン、ェチルセルロース、メチルセルロー  [0248] For example, gums and water-soluble polymer compounds include gum arabic, benzoin rubber, dammar gum, guayata fat, Irish moss, kala gum, tragacanth gum, kilob gum, quinseed, agar, casein, dextrin, gelatin, pectin, Starch, carrageen, chitosan salt, sulfated chitin, phosphorylated chitin, alginic acid and its salt, hyaluronic acid and its salt, chondroitin sulfate, heparin, ethyl cellulose, methylcellulose
ロセノレロース、結晶セノレロース、ポリビニノレアノレコーノレ、ポリビニノレメチノレエーテノレ、 ポリビュルピロリドン、ポリビュルメタアタリレート、ポリアクリル酸塩、ポリエチレンォキ サイドやポリプロピレンオキサイド等のポリアルキレンオキサイドまたはその架橋重合 物、カルボキシビュルポリマー、ポリエチレンィミンなどが挙げられる。 Rosenorelose, crystalline senorelose, polyvinylenorenoleconole, polyvinino methinoreatenore, polybulol pyrrolidone, polybull metaaterylate, polyacrylic acid salt, polyalkylene oxide such as polyethylene oxide and polypropylene oxide or cross-linking thereof Polymers, carboxybule polymers, polyethyleneimine and the like can be mentioned.
[0249] 例えば、界面活性剤としては、ァニオン界面活性剤（カルボン酸塩、スルホン酸塩、 硫酸エステル塩、リン酸エステル塩）、カチオン界面活性剤（ァミン塩、四級アンモニ ゥム塩）、両性界面活性剤（カルボン酸型両性界面活性剤、硫酸エステル型両性界 面活性剤、スルホン酸型両性界面活性剤、リン酸エステル型両性界面活性剤）、非ィ オン界面活性剤（エーテル型非イオン界面活性剤、エーテルエステル型非イオン界 面活性剤、エステル型非イオン界面活性剤、ブロックポリマー型非イオン界面活性剤 、含窒素型非イオン界面活性剤）、その他の界面活性剤 (天然界面活性剤、タンパク 質加水分解物の誘導体、高分子界面活性剤、チタン'ケィ素を含む界面活性剤、フ ッ化炭素系界面活性剤などが挙げられる。 [0249] For example, as the surfactant, an anionic surfactant (carboxylate, sulfonate, sulfate ester salt, phosphate ester salt), a cationic surfactant (ammine salt, quaternary ammonia) Um salt), amphoteric surfactant (carboxylic acid type amphoteric surfactant, sulfate ester type amphoteric surfactant, sulfonic acid type amphoteric surfactant, phosphate ester type amphoteric surfactant), nonionic surfactant Agents (ether type nonionic surfactant, ether ester type nonionic surfactant, ester type nonionic surfactant, block polymer type nonionic surfactant, nitrogen-containing type nonionic surfactant), other interfaces Activating agents (natural surfactants, derivatives of protein hydrolysates, polymeric surfactants, surfactants containing titanium'-carbon, fluorocarbon surfactants, etc.).
[0250] 例えば、ビタミン類としては、ビタミン A群ではレチノール、レチナール（ビタミン A1) 、デヒドロレチナール（ビタミン A2)、カロチン、リコピン（プロビタミン A)、ビタミン B群 では、チアミン塩酸塩、チアミン硫酸塩（ビタミン B1)、リボフラビン（  [0250] For example, as vitamins, retinol, retinal (vitamin A1), dehydroretinal (vitamin A2), carotene, lycopene (provitamin A), vitamin B group, thiamine hydrochloride, thiamine sulfate (Vitamin B1), riboflavin (
ビタミン B2)、ピリドキシン（ビタミン B6)、シァノコバラミン（ビタミン B12)、葉酸類、二 コチン酸類、パントテン酸類、ビォチン類、コリン、イノシトール類、ビタミン C群では、 ァスコルビン酸およびその誘導体、ビタミン D群では、ェルゴカルシフエロール（ビタミ ン D2)、コレカルシフエロール（ビタミン D3)、ジヒドロタキステロール、ビタミン E群で は、トコフエロールおよびその誘導体、ュビキノン類、ビタミン K群では、フイトナジオン (ビタミン K1)、メナキノン（ビタミン K2)、メナジオン（ビタミン K3)、メナジオール（ビタ ミン K4)などが挙げられる。  Vitamin B2), pyridoxine (vitamin B6), cyanocobalamin (vitamin B12), folic acid, nicotinic acid, pantothenic acid, biotin, choline, inositol, vitamin C group, ascorbic acid and its derivatives, vitamin D group In ergocalciferol (vitamin D2), cholecalciferol (vitamin D3), dihydrotachysterol, vitamin E group, tocopherol and its derivatives, ubiquinones, vitamin K group, futonadione (vitamin K1) Menaquinone (vitamin K2), menadione (vitamin K3), menadiol (vitamin K4), and the like.
[0251] 例えば、アミノ酸としては、ノ リン、ロイシン、イソロイシン、トレオニン、メチォニン、フ ェニルァラニン、トリプトファン、リジン、グリシン、ァラニン、ァスパラギン、グルタミン、 セリン、システィン、シスチン、チロシン、プロリン、ヒドロキシプロリン、ァスパラギン酸、 グノレタミン酸、ヒドロキシリジン、ァノレギニン、才ノレニチン、ヒスチジンなどや、それらの 硫酸塩、リン酸塩、硝酸塩、クェン酸塩、あるいはピロリドンカルボン酸の如きアミノ酸 誘導体などが挙げられる。  [0251] For example, amino acids include norine, leucine, isoleucine, threonine, methionine, phenylalanine, tryptophan, lysine, glycine, alanine, asparagine, glutamine, serine, cysteine, cystine, tyrosine, proline, hydroxyproline, and aspartic acid. And amino acid derivatives such as sulphates, phosphates, nitrates, citrates, pyrrolidone carboxylic acids, etc., and gnoretamic acid, hydroxylysine, anoleginine, norenitine and histidine.
[0252] 例えば、美白剤としては、ァスコルビン酸またはその誘導体、ィォゥ、胎盤加水分解 物、エラグ酸またはその誘導体、コウジ酸またはその誘導体、ダルコサミンまたはその 誘導体、アルブチンまたはその誘導体、ヒドロキシケィヒ酸またはその誘導体、グルタ チォン、ァ /レニ力エキス、ォゥゴンエキス、ソゥハクヒエキス、サイコエキス、ボウフゥェ キス、マンネンタケ菌糸体培養物またはその抽出物、シナノキエキス、モモ葉エキス、 エイジッエキス、クジンエキス、ジュエキス、トウキエキス、ョクイニンエキス、力キ葉ェ キス、ダイ才ゥエキス、ボタンピエキス、ノヽマメリスエキス、マロニエエキス、才トギリソゥ エキス、油溶性カンゾゥエキスなどが挙げられる。 [0252] For example, whitening agents include ascorbic acid or a derivative thereof, io, placental hydrolyzate, ellagic acid or a derivative thereof, kojic acid or a derivative thereof, darcosamine or a derivative thereof, arbutin or a derivative thereof, hydroxykehiic acid or a derivative thereof Derivatives such as glutathione, A / Leni force extract, gonon extract, sohakuhi extract, psycho extract, boufeque kiss, mynentake mycelium culture or extract thereof, linden extract, peach leaf extract, Examples include AGE extract, KUJIN extract, juju extract, toki extract, rye quinin extract, brilliant leaf kiss, dai sai extract, bot pi extract, cucumber bean extract, marronnier extract, virgin togiri extract, and oil-soluble licorice extract.
[0253] 例えば、保湿剤としては、ヒアルロン酸、ポリグルタミン酸、セリン、グリシン、スレオニ ン、ァラニン、コラーゲン、加水分角早コラーゲン、ヒドロネクチン、フイブロネクチン、ケ ラチン、エラスチン、ローヤルゼリー、コンドロイチン硫酸へパリン、グリセ口リン脂質、 グリセ口糖脂質、スフインゴリン脂質、スフインゴ糖脂質、リノール酸またはそのエステ ル類、エイコサペンタエン酸またはそのエステル類、ぺクチン、ビフィズス菌発酵物、 乳酸発酵物、酵母抽出物、レイシ菌糸体培養物またはその抽出物、小麦胚芽油、ァ ボガド油、米胚芽油、ホホバ油、ダイズリン脂質、 γ —オリザノール、ビロウドアオイェ キス、ョクイユンエキス、ジ才ゥエキス、タイソゥエキス、力イソゥエキス、キダチアロェェ キス、ゴボウエキス、マンネンロウエキス、アル二力エキス、小麦フスマなどが挙げられ [0253] For example, as a moisturizing agent, hyaluronic acid, polyglutamic acid, serine, glycine, threonine, alanine, collagen, hydrokeratin collagen, hydronectin, fibronectin, keratin, elastin, royal jelly, chondroitin sulfate heparin, Glyce mouth phospholipid, Glyce mouth phospholipid, Sphingo phospholipid, Sphingo glycolipid, Linoleic acid or its esters, Eicosapentaenoic acid or its esters, Pectin, Bifidobacterium fermented product, Lactic acid fermented product, Yeast extract, Ganoderma Mycelium culture or extract thereof, wheat germ oil, avogad oil, rice germ oil, jojoba oil, soybean phospholipid, γ — oryzanol, belode oyster kiss, yoku yun extract, di sai extract, taiso extract, force iso extract, kidachi araje kiss, Burdock Extracts, mannen wax extract, al niki extract, wheat bran etc.
[0254] 例えば、動物あるいは植物、生薬の抽出物やエキスとしては、ァセンャク（阿仙薬） 、ァシタバ、ァセロラ、ァノレテア、アル二力、アボカド、アマチヤ（甘茶）、アロエ、アロエ ベラ、イラクサ、イチヨウ (銀杏葉、銀杏）、ウイキヨゥ（茴香）、ゥコン (鬱金）、ウスパサイ シン (細辛）、ウメ（烏梅）、ゥラジ口ガシ、ゥワウルシ、ノイバラ（営実）、ヒキォコシ (延命 草）、ォウギ (黄耆）、コガネバナ (ォゥゴン）、ャマザクラ (桜皮）、キハダ (黄柏）、ォゥ レン (黄連）、ォタネニンジン（人参）、オトギリソゥ（弟切草）、ォドリコソゥ、オランダガラ シ、オレンジ、イトヒメハギ（遠志）、ゥッボダサ（夏枯草）、ツルドクダミ（何首烏）、ェン ジュ (槐花）、ョモギ (ガイ葉）、ガジュッ (莪朮）、クズ (葛根）、カノコソゥ（吉草根）、カミ ッレ、キカラスゥリ（瓜呂根）、力ワラョモギ（茵チン蒿）、カンゾゥ（甘草）、フキタンポポ (款冬花、款冬葉）、キイチゴ、キウイ果実、キキヨウ (桔梗）、キク (菊花）、キササゲ（ 梓実）、ミカン属植物果実 (枳実）、タチバナ (橘皮)、キユウリ、ウドまたはシシウド (羌活 、独活）、アンズ (杏仁）、クコ（地骨皮、枸杞子、枸杞葉）、クララ（苦参）、タスノキ、ク マザサ、グレープフルーツ果実、二ッケィ (桂皮）、ケィガイ (ケィガイ）、ェビスダサ (決 明子）、マルバアサガオまたはアサガオ (ケン牛子）、ベニバナ（紅花）、ゴバイシ（五 倍子）、コンフリー、コパイバ、クチナシ（山梔子）、ゲンチアナ、ホオノキ (厚朴）、ヒナ タイノコズチ（牛膝）、ゴシュュ（呉茱萸）、ゴボウ、チョウセンゴミシ (五味子）、米、米ぬ 力、、コムギ、ミシマサイコ（柴胡）、サフラン、サボンソゥ、サンザシ（山ザ子）、サンショウ (山椒）、サルビア、サンシチニンジン（三七人参）、シィタケ (椎茸）、ジォゥ（地黄）、 シクンシ (使君子）、ムラサキ (紫根)、シソ (紫蘇葉、紫蘇子）、カキ (柿蒂）、シャクャク（ 芍薬）、ォォバコ（車前子、車前草）、ショウガ (生姜）、ショウブ (菖蒲）、トウネズミモチ (女貞子）、シモッケソゥ、シラカバ、スイカズラ（金銀花、忍冬）、セィヨウキヅタ、セィ ヨウノコギリソゥ、セィヨウニヮトコ、ァズキ（赤小豆）、ニヮトコ（接骨木）、ゼニァオイ、セ ンキユウ (川キユウ）、センプリ（当薬）、クヮ（桑白皮、桑葉）、ナツメ (大棗）、ダイズ、タ ラノキ、チクセッニンジン (竹節人参）、ハナスゲ (知母）、ヮレモコゥ（地楡）、ドクダミ（ 十薬）、フユムシナツクサタケ (冬虫夏草）、トウガラシ、ホオズキ (登呂根）、タチジヤコ ゥソゥ、リヨクチャ（緑茶）、コウチヤ (紅茶）、チヨウジ (丁子）、ゥンシユウミカン (陳皮）、 ツバキ、ッボクサ、トウガラシ (番椒）、トウキ（当帰）、トウキンセン力、ダイダイ (橙皮）、 ヮレモコゥ（地楡）、トウモロコシ（南蛮毛）、トチュウ（杜仲、杜仲葉）、トマト、ナンテン（ 南天実）、ニンニク (大サン）、ォォムギ (麦芽）、ハクセン（白蘚皮）、ジヤノヒゲ (麦門 冬）、パセリ、バタタ、ハツ力（薄荷）、ハマメリス、バラ、ビヮ葉 (枇杷葉）、マツホド（茯リ ヨウ）、ブドウまたはその葉、へチマ、ボダイジュ、ボタン (牡丹皮）、ホップ、マイカイ（ マイ瑰花）、松葉、マロニエ、マンネンロウ、ムクロジ、メリッサ、メリロート、ボケ（木瓜） 、モヤシ、モモ（桃仁、桃葉）、ヒォウギ (射干）、ビンロウジュ（檳ロウ子）、メハジキ（益 母草）、ャグルマギク、ユキノシタ（虎耳草）、ャマモモ（楊梅皮）、ヤシャブシ (矢車）、 ノヽトムギ（ョクイニン）、モウコョモギ、ャマョモギ、ラベンダー、リンゴ果実、マンネンタ ケ（霊芝）、レモン果実、レンギヨゥ（連翹）、レンゲソゥ、ゲンノショウコ（老鸛草）、ハシ リドコ口（ロート根）、鶏トサ力、牛'人の胎盤抽出物、豚'牛の胃、十二指腸、或いは腸 の抽出物若しくはその分解物、水溶性コラーゲン、水溶性コラーゲン誘導体、コラー ゲン加水分解物、エラスチン、エラスチン加水分解物、水溶性エラスチン誘導体、シ ルク蛋白、シルク蛋白分解物、牛血球蛋白分解物などが挙げられる。 [0254] For example, extracts or extracts of animals, plants, or herbal medicines include Azenak (Asenyaku), Ashitaba, Acerola, Anolethea, Arni, Avocado, Amatya (Amacha), Aloe, Aloe Vera, Nettle, Ichiyo ( Ginkgo biloba, Ginkgo), Wikiyo (Yuka), Ukon (Usukin), Uspasai Shin (Spicy), Ume (Plum), Vulture, Ganoderma, Neubara (Yutoshi), Hikikoshi (Extended Life Grass), Ogi (Yellow) , Koganebana (Ogon), Chestnut cherry (cherry bark), Kihada (Yellow), Oren (Yellow), Ginseng (Ginseng), Otogirisou (Little Cut Grass), Odoricosou, Dutch Garlic, Orange, Itohimehagi (Distance), Ubbodasa Summer hay), Tsurudukudami (what neck moth), Yen Ju (槐花), Momogi (guy leaf), Gajutsu (莪 朮), Kudzu (Kazune), Kano Kosou (Kisakune), Camille, Kikarasuri (Gorne), Power Warorogomi (Ginseng Chin), Ganzo (Lancho), Fukidan Popo (Chifu Winter Flower, Chou Fuyu), Raspberry, Kiwi Fruit, Kikiyo (Kikyo), Chrysanthemum (Chrysanthemum flower), Catalpa (Cultivation), Citrus fruit (Cultivation), Tachibana (Tachibana bark), Cucumber, Udo or Shishiudo (Cultivation, Independence), Apricot (Apricot jelly), Guco Leaf), Clara (bitter ginseng), Tasnoki, Kumazasa, grapefruit fruit, Nikkei (Kai-Kai), Kaigai (Kai-Gai), Yebisuda (Keiko), Malva morning glory or morning glory (Ken cow), safflower (Safflower), Gobaishi ( Quintuplet), comfrey, copaiba, gardenia (mountain lion), gentian, honoki (shoku), chick Tainokozuchi (Gyu-knee), Goshu (Kurean), Burdock, Korean corn (Gomiko), Rice, Rice bran, Wheat, Mishimasaiko (Shibahu), Saffron, Savonso, Hawthorn (Yamazako), Sansho ), Salvia, Sancynin carrot (37 ginseng), Shitake (shiitake), Jio (jihuang), Sikunshi (messenger), Murasaki (purple root), Perilla (purple leaf, Shisoko), Oyster (salmon), Peonies (Glaze), wobco (car pup, car front grass), ginger (ginger), shobu (cage), horned mochi (woman Sadako), shimokso, birch, honeysuckle (gold and silver flowers, Shinobu), seiyokita, seiyo nokogirisou, seiyozuki (Red azuki beans), Nito-toko (bone grafts), Zeniaoi, Senkiyu (river kiyu), Senpuri (this medicine), cucumber (mulberry white skin, mulberry leaves), jujube (Daegu), Soybean, Taranki, Chikusenninjin (Takebushi Ginseng), Hanasuge (My mother), Samurai Remokoku (Domestic), Dokudami (Decay), Fuyuminatsusakutake (Cycium Cordyceps), Capsicum, Japanese physalis (Tororoot) ), Tachijiyako Usou, Ryokucha (green tea), Kouchiya (tea), Chiyouji (chome), Unshiyumikan (Chen), Camellia, Duckweed, Capsicum (Banban), Touki (Toshiki), Tokinsen power, Daidai (orange peel),ヮ Remokō (corn), corn (southern cocoon), eucommia (shunchu, tsunakaba), tomato, nanten (southern tenmi), garlic (large sun), barley (malt), hakusen (white husk), jiyahige (makumon) Winter), Parsley, Batata, Heart force (light load), Hamamelis, Rose, Bi-leaf (leaf), Matsuhodo, Grape or its leaves, Loofah, Bodai , Buttons (peony skin), hops, maikai (my buds), pine needles, marronnier, mannenrou, mukuroji, melissa, merirot, bokeh, coconut palm, peaches (peach seeds, peach leaves), hyogi (shooting), areca檳 Rouko), Mejijiki (beneficial mother grass), cornflower, yukinoshita (tiger ear grass), octopus ginseng, yashabushi (arrow wheel), cypress cypress (mushroom), mugwort moth, cinnamon moth, lavender, apple fruit, mannentake ( Ganoderma), lemon fruit, forsythia, rengesou, geno shoko (old-growth grass), hashidoko mouth (roth root), chicken toss, cow's placenta extract, pig's stomach, duodenum, or Intestinal extract or its degradation product, water-soluble collagen, water-soluble collagen derivative, collagen hydrolyzate, elastin, elastin Water decomposition products, water-soluble elastin derivative, shea torque protein, silk protein hydrolyzate, and the like bovine hemocyte protein hydrolyzate.
例えば、微生物培養代謝物としては、酵母エキス、亜鉛含有酵母エキス、ゲルマ二 ゥム含有酵母エキス、セレン含有酵母エキス、マグネシウム含有酵母エキス、米醱酵 エキス、ユーグレナ抽出物、脱脂粉乳の乳酸発酵物などが挙げられる。 [0256] 例えば、 α —ヒドロキシ酸としては、グリコーノレ酸、クェン酸、リンゴ酸、酒石酸、乳 酸などが挙げられる。 For example, microbial culture metabolites include yeast extract, zinc-containing yeast extract, germanium-containing yeast extract, selenium-containing yeast extract, magnesium-containing yeast extract, rice fermentation extract, Euglena extract, and lactic acid fermented milk powder. Etc. [0256] For example, examples of α-hydroxy acids include glyconolic acid, citrate, malic acid, tartaric acid, and lactic acid.
[0257] 例えば、無機顔料としては、無水ケィ酸、ケィ酸マグネシウム、タルク、カオリン、ベ ントナイト、マイ力、雲母チタン、ォキシ塩化ビスマス、酸化ジルコニウム、酸化マグネ シゥム、酸化亜鉛、酸化チタン、炭酸カルシウム、炭酸マグネシウム、黄酸化鉄、ベン ガラ、黒酸化鉄、グンジヨウ、酸化クロム、水酸化クロム、カーボンブラック、カラミンな どが挙げられる。  [0257] For example, as inorganic pigments, anhydrous caustic acid, magnesium silicate, talc, kaolin, bentonite, my strength, mica titanium, bismuth oxychloride, zirconium oxide, magnesium oxide, zinc oxide, titanium oxide, calcium carbonate Magnesium carbonate, yellow iron oxide, red iron oxide, black iron oxide, gundiio, chromium oxide, chromium hydroxide, carbon black, calamine and the like.
[0258] 例えば、紫外線吸収剤としては、 ρ—ァミノ安息香酸誘導体、サルチル酸誘導体、 アントラニル酸誘導体、クマリン誘導体、アミノ酸系化合物、ベンゾトリアゾール誘導 体、テトラゾール誘導体、イミダゾリン誘導体、ピリミジン誘導体、ジォキサン誘導体、 カンファー誘導体、フラン誘導体、ピロン誘導体、核酸誘導体、アラントイン誘導体、 ニコチン酸誘導体、ビタミン Β6誘導体、ォキシベンゾン、ベンゾフエノン、グアイァズレ ン、シコニン、バイカリン、ノイカレイン、ベルべリンなどが挙げられる。  [0258] For example, as an ultraviolet absorber, ρ-aminobenzoic acid derivative, salicylic acid derivative, anthranilic acid derivative, coumarin derivative, amino acid compound, benzotriazole derivative, tetrazole derivative, imidazoline derivative, pyrimidine derivative, dioxane derivative, Examples include camphor derivatives, furan derivatives, pyrone derivatives, nucleic acid derivatives, allantoin derivatives, nicotinic acid derivatives, vitamin 6 derivatives, oxybenzone, benzophenone, guaiazurene, shikonin, baicalin, noikalein, and berberine.
[0259] 例えば、収斂剤としては、乳酸、酒石酸、コハク酸、クェン酸、アラントイン、塩化亜 鉛、硫酸亜鉛、酸化亜鉛、カラミン、 ρ—フエノールスルホン酸亜鉛、硫酸アルミニウム カリウム、レソルシン、塩化第二鉄、タンニン酸などが挙げられる。  [0259] For example, astringents include lactic acid, tartaric acid, succinic acid, succinic acid, allantoin, lead chloride, zinc sulfate, zinc oxide, calamine, ρ-phenol sulfonate zinc, potassium aluminum sulfate, resorcin chloride, secondary chloride Examples thereof include iron and tannic acid.
[0260] 例えば、抗酸化剤としては、ァスコルビン酸およびその塩、ステアリン酸エステル、ト コフエロールおよびそのエステル誘導体、ノルジヒドログァセレテン酸、ブチルヒドロキ シトルエン（ΒΗΤ)、ブチルヒドロキシァ二ソール（ΒΗΑ)、パラヒドロキシァニソール、 没食子酸プロピル、セサモール、セサモリン、ゴシポールなどが挙げられる。  [0260] For example, antioxidants include ascorbic acid and its salts, stearic acid ester, tocopherol and its ester derivatives, nordihydroguasertenic acid, butylhydroxytoluene (ΒΗΤ), butylhydroxydisole (ΒΗΑ) , Parahydroxyanisole, propyl gallate, sesamol, sesamorin, gossypol and the like.
[0261] 例えば、抗炎症剤としては、ィクタモール、インドメタシン、カオリン、サリチル酸、サ リチル酸ナトリウム、サリチル酸メチル、ァセチルサリチル酸、塩酸ジフェンヒドラミン、 dまたは dl—力ンフノレ、ヒドロコルチゾン、グアイァズレン、カマズレン、マレイン酸クロ ルフエ二ラミン、グリチルリチン酸およびその塩、グリチルレチン酸およびその塩など が挙げられる。  [0261] For example, anti-inflammatory agents include ictamol, indomethacin, kaolin, salicylic acid, sodium salicylate, methyl salicylate, acetylsalicylic acid, diphenhydramine hydrochloride, d or dl-forcenfunole, hydrocortisone, guaiazulene, camazulene, maleic acid chloro Examples thereof include lucerilamine, glycyrrhizic acid and its salt, glycyrrhetinic acid and its salt, and the like.
[0262] 例えば、殺菌 '消毒薬としては、アタリノール、ィォゥ、塩化ベンザルコニゥム、塩化 ベンゼトニゥム、塩化メチルロザ二リン、クレゾ一ノレ、ダルコン酸カルシウム、ダルコン 酸クロルへキシジン、スルファミン、マーキュロクロム、ラタトフエリンまたはその加水分 解物などが挙げられる。 [0262] For example, disinfectants include atalinol, io, benzalkonium chloride, benzethonium chloride, methyl rosaniline chloride, cresol monole, calcium dalconate, chlorhexidine dalconate, sulfamine, mercurochrome, ratatoferrin or its addition. moisture Examples include scraps.
[0263] 例えば、頭髪用剤としては、二硫化セレン、臭化アルキルイソキノリニゥム液、ジンク ピリチ才ン、ビフエナミン、チアントール、カスタリチンキ、ショウキヨウチンキ、トウガラシ チンキ、塩酸キニーネ、強アンモニア水、臭素酸カリウム、臭素酸ナトリウム、チォダリ コール酸などが挙げられる。  [0263] For example, as a hair preparation, selenium disulfide, alkylisoquinolinium bromide, zinc pilichin, bifuenamine, thianthol, castari tincture, pepper tincture, pepper tincture, quinine hydrochloride, strong ammonia water, Examples include potassium bromate, sodium bromate, and thiodaricholic acid.
[0264] 例えば、香料としては、ジヤコゥ、シベット、カストリウム、アンバーグリスなどの天然 動物性香料、ァニス精油、アングリ力精油、イラン精油、ィリス精油、ウイキヨゥ精油、 オレンジ精油、カナンガ精油、力ラウエ一精油、カルダモン精油、グアヤタウッド精油 、クミン精油、黒文字精油、ケィ皮精油、シンナモン精油、ゲラニゥム精油、コパイバ バルサム精油、コリアンデル精油、シソ精油、シダーウッド精油、シトロネラ精油、ジャ スミン精油、ジンジャーグラス精油、杉精油、スペアミント精油、西洋ハツ力精油、大茴 香精油、チュべローズ精油、丁字精油、橙花精油、冬緑精油、トルーバルサム精油、 バチユリ一精油、バラ精油、パルマローザ精油、檜精油、ヒバ精油、白檀精油、プチ ダレン精油、べィ精油、べチバ精油、ベルガモット精油、ペルーバルサム精油、ボア ドローズ精油、芳樟精油、マンダリン精油、ユーカリ精油、ライム精油、ラベンダー精 油、リナロェ精油、レモングラス精油、レモン精油、ローズマリー精油、和種ハツ力精 油などの植物性香料、その他合成香料などが挙げられる。  For example, as perfumes, natural animal perfumes such as Jiakou, Civet, Castrium, Ambergris, Anise Essential Oil, Angri Essential Oil, Iran Essential Oil, Iris Essential Oil, Wikiyo Essential Oil, Orange Essential Oil, Kananga Essential Oil, Power Laue Essential Oil , Cardamom essential oil, guayata wood essential oil, cumin essential oil, black letter essential oil, key skin essential oil, cinnamon essential oil, geranium essential oil, copaiba balsam essential oil, coriandel essential oil, perilla essential oil, cedarwood essential oil, citronella essential oil, jasmine essential oil, gingergrass essential oil, cedar essential oil , Spearmint essential oil, Western heart force essential oil, Otsuka perfume essential oil, tuberose essential oil, clove essential oil, orange flower essential oil, winter green essential oil, trout balsam essential oil, batile lily essential oil, rose essential oil, palmarosa essential oil, persimmon essential oil, hiba essential oil, Sandalwood essential oil, Petite Daren essential oil, ve essential oil, vetiver Oil, bergamot essential oil, Peruvian balsam essential oil, bored rose essential oil, mandarin essential oil, mandarin essential oil, eucalyptus essential oil, lime essential oil, lavender essential oil, linaloe essential oil, lemongrass essential oil, lemon essential oil, rosemary essential oil, Japanese varieties essential oil And other synthetic flavors.
[0265] 例えば、色素 ·着色剤としては、赤キャベツ色素、赤米色素、ァカネ色素、アナトー 色素、イカスミ色素、ゥコン色素、ェンジュ色素、ォキアミ色素、柿色素、カラメル、金 、銀、クチナシ色素、コーン色素、タマネギ色素、タマリンド色素、スピルリナ色素、ソ バ全草色素、チェリー色素、海苔色素、ハイビスカス色素、ブドウ果汁色素、マリーゴ 一ルド色素、紫ィモ色素、紫ャマイモ色素、ラック色素、ルチンなどが挙げられる。  [0265] For example, as a colorant / colorant, red cabbage dye, red rice dye, akane dye, anato dye, squid ink dye, turmeric dye, chenju dye, krill dye, amber dye, caramel, gold, silver, gardenia dye, Corn pigment, onion pigment, tamarind pigment, spirulina pigment, buckwheat whole plant pigment, cherry pigment, laver pigment, hibiscus pigment, grape juice pigment, marigo mulberry pigment, purple imo pigment, purple potato pigment, lac pigment, rutin, etc. Is mentioned.
[0266] 例えば、甘味料としては、砂糖、甘茶、果糖、ァラビノース、ガラクトース、キシロース 、マンノース、麦芽糖、蜂蜜、ブドウ糖、ミラクリン、モネリンなどが挙げられる。  [0266] Examples of the sweetener include sugar, sweet tea, fructose, arabinose, galactose, xylose, mannose, maltose, honey, glucose, miraculin, monelin and the like.
[0267] 例えば、栄養強化剤としては、貝殻焼成カルシウム、シァノコラバミン、酵母、小麦 胚芽、大豆胚芽、卵黄粉末、へミセルロース、ヘム鉄などが挙げられる。  [0267] For example, examples of the nutrient fortifier include shell calcined calcium, cyanobactamine, yeast, wheat germ, soybean germ, egg yolk powder, hemicellulose, heme iron and the like.
[0268] その他、ホルモン類、金属イオン封鎖剤、 pH調整剤、キレート剤、防腐 .防バイ剤、 清涼剤、安定化剤、乳化剤、動 ·植物性蛋白質およびその分解物、動 ·植物性多糖 類およびその分解物、動'植物性糖蛋白質およびその分解物、血流促進剤、消炎剤 *抗アレルギー剤、細胞賦活剤、角質溶解剤、創傷治療剤、増泡剤、増粘剤、口腔 用剤、消臭 ·脱臭剤、苦味料、調味料、酵素などが挙げられる。 [0268] Others, hormones, sequestering agents, pH adjusters, chelating agents, antiseptics, antibacterial agents, refreshing agents, stabilizers, emulsifiers, animal / plant proteins and their degradation products, animal / plant polysaccharides And its degradation products, animal and plant glycoproteins and their degradation products, blood flow promoters, anti-inflammatory agents * Anti-allergic agents, cell activators, keratolytic agents, wound treatment agents, foam enhancers, thickeners, oral cavity Preparations, deodorants / deodorants, bitters, seasonings, enzymes, etc.
[0269] 本発明の第 2の発明に係る賦活化剤または本発明の第 4の発明に係るコラーゲン 産生促進剤は、ヒトに対して好適に適用されるものである力 それぞれの作用効果が 期待できる限り、ヒト以外の動物に対して適用することもできる。  [0269] The activator according to the second invention of the present invention or the collagen production promoter according to the fourth invention of the present invention is one that is suitably applied to humans. As far as possible, it can also be applied to non-human animals.
[0270] 本発明の第 2の発明に係る賦活化剤または本発明の第 4の発明に係るコラーゲン 産生促進剤は、特に限定されるものではないが、非イオン性の界面活性剤や低級ァ ルコール、多価アルコール、あるいはォリーブ油、スクヮラン、脂肪酸などの天然油脂 に溶解して用いることができる。  [0270] The activator according to the second invention of the present invention or the collagen production promoter according to the fourth invention of the present invention is not particularly limited, but a nonionic surfactant or lower alcohol is not particularly limited. It can be used by dissolving in alcohol, polyhydric alcohol, or natural oils such as olive oil, squalane and fatty acids.
[0271] 非イオン界面活性剤としては、例えば、ソルビタン脂肪酸エステル類 (例えば、ソル ビタンモノォレエート、ソルビタンモノイソステアレート、ソルビタンモノラウレート、ソル ビタンモノパルミテート、ソルビタンモノステアレート、ソルビタンセスキォレエート、ソ ノレビタントリオレエート、ペンター 2—ェチルへキシル酸ジグリセロールソルビタン、テ トラー 2—ェチルへキシル酸ジグリセロールソルビタン等）；グリセリンポリグリセリン脂 肪酸類 (例えば、モノ綿実油脂肪酸グリセリン、モノエル力酸グリセリン、セスキォレイ ン酸グリセリン、モノステアリン酸グリセリン、 α , α，ーォレイン酸ピログルタミン酸ダリ セリン、モノステアリン酸グリセリンリンゴ酸等）；プロピレングリコール脂肪酸エステル 類（例えば、モノステアリン酸プロピレングリコール等）；硬化ヒマシ油誘導体；グリセリ ンアルキルエーテル等が挙げられる。  [0271] Examples of the nonionic surfactant include sorbitan fatty acid esters (for example, sorbitan monooleate, sorbitan monoisostearate, sorbitan monolaurate, sorbitan monopalmitate, sorbitan monostearate, sorbitan). Sesquioleate, sonolebitan trioleate, pentater 2-ethyl hexyl diglycerol sorbitan, tetra 2-ethyl hexyl diglycerol sorbitan, etc.); glycerin polyglycerin fatty acids (eg mono cottonseed oil fatty acid glycerin, Glyceryl monoellate, glyceryl sesquioleate, glyceryl monostearate, α, α, oleic acid pyroglutamate darice, monostearate glycerin malate, etc.); propylene glycol fatty acid esters (eg For example, propylene glycol monostearate); hardened castor oil derivative; glycerin alkyl ether and the like.
[0272] ΡΟΕ系の親水性非イオン界面活性剤としては、例えば、 ΡΟΕ—ソルビタン脂肪酸ェ ステル類（例えば、 ΡΟΕ—ソルビタンモノォレエート、 ΡΟΕ—ソルビタンモノステアレ ート、 ΡΟΕ—ソルビタンモノォレート、 ΡΟΕ—ソルビタンテトラオレエート等）； ΡΟΕソ ルビット脂肪酸エステル類（例えば、 ΡΟΕ—ソルビットモノラウレート、 ΡΟΕ—ソルビッ トモノォレエート、 ΡΟΕ—ソルビットペンタォレエート、 ΡΟΕ—ソルビットモノステアレ ート等）； ΡΟΕ—グリセリン脂肪酸エステル類（例えば、 ΡΟΕ—グリセリンモノステアレ ート、 ΡΟΕ—グリセリンモノイソステアレート、 ΡΟΕ—グリセリントリイソステアレート等 の ΡΟΕ—モノォレエート等）； ΡΟΕ—脂肪酸エステル類（例えば、 ΡΟΕ—ジステアレ ート、 POE モノジォレエート、ジステアリン酸エチレングリコール等）； POE アルキ ノレエーテル類（例えば、 POE ラウリルエーテル、 POE ォレイルエーテル、 POE ーステアリルエーテル、 POE べへニルエーテル、 POE— 2—オタチルドデシルェ 一テル、 POE コレスタノールエーテル等）；プル口ニック型類（例えば、プル口ニック 等）； ΡΟΕ· POP アルキルエーテル類（例えば、 ΡΟΕ· POP セチルエーテル、 P OE · POP— 2—デシルテトラデシルエーテル、 POE · POP—モノブチルエーテル、 POE'POP 水添ラノリン、 POE'POP グリセリンエーテル等）；テトラ POE.テトラ POP エチレンジァミン縮合物類（例えば、テトロニック等）； POE ヒマシ油硬化ヒ マシ油誘導体（例えば、 POE ヒマシ油、 POE 硬化ヒマシ油、 POE 硬化ヒマシ 油モノイソステアレート、 POE 硬化ヒマシ油トリイソステアレート、 POE 硬化ヒマシ 油モノピログルタミン酸モノイソステアリン酸ジエステル、 POE 硬化ヒマシ油マレイ ン酸等）； POE ミツロウ'ラノリン誘導体（例えば、 POE ソルビットミツロウ等）；アル 力ノールアミド（例えば、ヤシ油脂肪酸ジエタノールアミド、ラウリン酸モノエタノールァ ミド、脂肪酸イソプロパノールアミド等）； POE プロピレングリコール脂肪酸エステル ； POE -アルキルアミン； POE 脂肪酸アミド；ショ糖脂肪酸エステル；アルキルエト キシジメチルアミンォキシド；トリオレィルリン酸等が挙げられる。 [0272] Examples of the hydrophilic hydrophilic nonionic surfactant include 、 -sorbitan fatty acid esters (for example, ΡΟΕ-sorbitan monooleate, ΡΟΕ-sorbitan monostearate, ΡΟΕ-sorbitan monoo). 、, sorbitan tetraoleate, etc .; ΡΟΕ sorbite fatty acid esters (eg, ΡΟΕ—sorbite monolaurate, ΡΟΕ—sorbite monooleate, ΡΟΕ—sorbitol pentaoleate, ΡΟΕ—sorbite monostearate, etc.) ΡΟΕ-glycerin fatty acid esters (eg, ΡΟΕ-glycerol monostearate, ΡΟΕ-glycerol monoisostearate, ΡΟΕ-glycerol triisostearate, etc.); ； -fatty acid esters (eg, ΡΟΕ—Disteare , POE monodiolate, ethylene glycol distearate, etc .; POE alkynole ethers (eg, POE lauryl ether, POE oleyl ether, POE-stearyl ether, POE behenyl ether, POE-2-Otachidodecyl ether, POE cholestanol ether, etc.); Pull-neck type (eg, pull-mouth nick), etc .; ΡΟΕ · POP alkyl ethers (eg, ΡΟΕ · POP cetyl ether, P OE · POP-2-decyl tetradecyl ether, POE · POP-monobutyl ether, POE'POP hydrogenated lanolin, POE'POP glycerin ether, etc.); Tetra POE. Tetra POP ethylene diamine condensates (eg, Tetronic etc.); POE castor oil hardened castor oil derivatives (eg, POE castor) Oil, POE hydrogenated castor oil, POE hydrogenated castor oil monoisostearate, POE Castor oil triisostearate, POE hydrogenated castor oil monopyroglutamic acid monoisostearic acid diester, POE hydrogenated castor oil maleic acid, etc.); POE beeswax 'lanolin derivatives (eg POE sorbite beeswax etc.); Coconut oil fatty acid diethanolamide, lauric acid monoethanolamide, fatty acid isopropanolamide, etc.); POE propylene glycol fatty acid ester; POE-alkylamine; POE fatty acid amide; sucrose fatty acid ester; alkylethoxydimethylamine oxide; trioleyl phosphoric acid, etc. Can be mentioned.
[0273] 低級アルコールとしては、例えば、エタノール、プロパノール、イソプロパノール、イソ ブチルアルコール、 t ブチルアルコール等が挙げられる。  [0273] Examples of the lower alcohol include ethanol, propanol, isopropanol, isobutyl alcohol, t-butyl alcohol, and the like.
[0274] 多価アルコールとしては、例えば、 2価のアルコール（例えば、エチレングリコール、 プロピレングリコール、トリメチレングリコール、 1 , 2—ブチレングリコール、 1 , 3—ブチ レングリコーノレ、テトラメチレングリコーノレ、 2, 3 ブチレングリコーノレ、ペンタメチレン グリコーノレ、 2 ブテン 1 , 4 ジォーノレ、へキシレングリコーノレ、オタチレングリコー ル等）；3価のアルコール（例えば、グリセリン、トリメチロールプロパン等）；4価アルコ ール（例えば、 1 , 2, 6 へキサントリオール等のペンタエリスリトール等）； 5価アルコ ール（例えば、キシリトール等）；6価アルコール（例えば、ソノレビトーノレ、マンニトーノレ 等）；多価アルコール重合体（例えば、ジエチレングリコール、ジプロピレングリコール 、トリエチレングリコール、ポリプロピレングリコール、テトラエチレンダリコール、ジグリ セリン、ポリエチレングリコール、トリグリセリン、テトラグリセリン、ポリグリセリン等）；2価 のアルコールアルキルエーテル類（例えば、エチレングリコールモノメチルエーテル、 エチレングリコーノレモノェチノレエーテノレ、エチレングリコーノレモノブチノレエーテノレ、ェ チレングリコ一ノレモノフエニノレエーテノレ、エチレングリコ一ノレモノへキシノレエーテノレ、 エチレングリコーノレモノ 2—メチノレへキシノレエーテノレ、エチレングリコーノレイソアミノレェ ーテノレ、エチレングリコーノレべンジノレエーテノレ、エチレングリコーノレイソプロピノレエ一 テノレ、エチレングリコーノレジメチノレエーテノレ、エチレングリコーノレジェチノレエーテノレ、 エチレングリコールジブチルエーテル等）； 2価アルコールアルキルエーテル類（例え ば、ジエチレングリコーノレモノメチノレエーテノレ、ジエチレングリコーノレモノェチノレエー テル、ジエチレングリコールモノブチルエーテル、ジエチレングリコールジメチルエー テノレ、ジエチレングリコーノレジェチノレエーテノレ、ジエチレングリコーノレブチノレエーテ ノレ、ジエチレングリコーノレメチノレエチノレエーテノレ、 トリエチレングリコーノレモノメチノレエ ーテノレ、トリエチレングリコーノレモノェチノレエーテノレ、プロピレングリコーノレモノメチノレ エーテノレ、プロピレングリコーノレモノェチノレエーテノレ、プロピレングリコ一ノレモノブチ ノレエーテノレ、プロピレングリコーノレイソプロピノレエーテノレ、ジプロピレングリコ一ノレメチ ノレエーテノレ、ジプロピレングリコーノレエチノレエーテノレ、ジプロピレングリコーノレブチノレ エーテル等）；2価アルコールエーテルエステル（例えば、エチレングリコールモノメチ ルエーテルアセテート、エチレングリコールモノェチルエーテルアセテート、エチレン グリコーノレモノブチノレエーテノレアセテート、エチレングリコーノレモノフエニノレエーテノレ アセテート、エチレングリコーノレジアジべート、エチレングリコーノレジサクシネート、ジ エチレングリコールモノェチルエーテルアセテート、ジエチレングリコールモノブチル エーテノレアセテート、プロピレングリコーノレモノメチノレエーテノレアセテート、プロピレン グリコーノレモノェチノレエーテノレアセテート、プロピレングリコーノレモノプロピノレエーテ ルアセテート、プロピレングリコールモノフエニルエーテルアセテート等）；グリセリンモ ノアルキルエーテル（例えば、キミノレァノレコーノレ、セラキルアルコール、バチルアルコ ール等）；糖アルコール（例えば、ソノレビトーノレ、マルチトーノレ、マノレトトリオース、マン 二トーノレ、ショ糖、エリトリトーノレ、グルコース、フルクトース、デンプン分解糖、マルトー ス、キシリトース、デンプン分解糖還元アルコール等）；グリソリッド；テトラハイド口フル フリノレアノレコーノレ； POE—テトラハイド口フルフリルアルコール； POP—ブチルエーテ ノレ； POP · POE ブチノレエーテノレ；トリポリォキシプロピレングリセリンエーテノレ； POP -グリセリンエーテル； POP -グリセリンエーテルリン酸； POP · POE -ペンタンエリス リトールエーテル、ポリグリセリン等が挙げられる。 [0274] Examples of the polyhydric alcohol include divalent alcohols (for example, ethylene glycol, propylene glycol, trimethylene glycol, 1,2-butylene glycol, 1,3-butylene glycolol, tetramethylene glycolol, 2, 3 Butylene glycolanol, pentamethylene glycolanol, 2 butene 1,4 dianol, hexylene glycolanol, octylene glycol, etc .; trivalent alcohol (eg, glycerin, trimethylolpropane, etc.); tetravalent alcohol (eg, 1, 2, 6 hexanetriol, etc. pentaerythritol, etc.); pentavalent alcohol (eg, xylitol, etc.); hexavalent alcohol (eg, sonorebitanol, mannitol, etc.); polyhydric alcohol polymer (eg, diethylene glycol, diethylene glycol, etc.) Propylene glycol Ethylene glycol, polypropylene glycol, tetraethylene da recall, diglycidyl serine, polyethylene glycol, triglycerol, tetraglycerol, polyglycerol, etc.); divalent Alcohol alkyl ethers (for example, ethylene glycol monomethyl ether, ethylene glycol monoethyl enoate ethere, ethylene glyco mono mono butyl mono ether, ethylene glycol mono mono mono eno mono ether, Tenoré, Ethyleneglycolenomono 2-Methylenohexenoleethenore, Ethyleneglycolenoisoaminolethenore, Ethyleneglycolenovenenoleatenore, Ethyleneglycolenoisopropinoleene Tenole, Ethyleneglycolenoresininotenolate, ethylene Glycolic leseno chinenoate ethere, ethylene glycol dibutyl ether, etc.); Dihydric alcohol alkyl ethers (eg diethylene gluconole monomethino reetenol, di Tylene glycol monoethyl ether, diethylene glycol monobutyl ether, diethylene glycol dimethyl ether, diethylene glycol noretino chienore, diethylene glycol nole butino noate, diethylene glycol nole methino retinoate nore, triethylene glycol nore Monomethylenotenole, Triethyleneglycolenomonotenoleetenore, Propyleneglycololemonomethinoreatenore, Propyleneglycolenomonotenoleatenore, Propyleneglycolenolenomonobutyinoreatenore, Propyleneglycolenoisopropinoateenore, Di Propylene glycol monoremethinoreatenore, dipropyleneglycolenoretinoleatere, dipropyleneglycolenorebutinore Dihydric alcohol ether esters (for example, ethylene glycol monomethyl ether acetate, ethylene glycol monoethyl ether acetate, ethylene glycol monobutino oleate acetate, ethylene glycol mono mono enoate acetate, ethylene glycol eno) Residibate, Ethylene Glycono Resin Succinate, Diethylene Glycol Monoethyl Ether Acetate, Diethylene Glycol Monobutyl Etherenoacetate, Propylene Glyconore Monomethinoatenoreacetate, Propylene Glyconoremonoethylenoleetenoate Acetate, Propylene glycol monopropynolate acetate, propylene glycol monophenyl ether acetate, etc.); Monoalkyl ethers (eg, minomino ethanol, cerakil alcohol, batyl alcohol, etc.); sugar alcohols (eg, sonolebitonore, multitonole, manoletotriose, mannitotonole, sucrose, erythritole, glucose, fructose, Starch-degrading sugar, maltose, xylitol, starch-degrading sugar reducing alcohol, etc.); Glysolid; Tetrahide mouth full frinorenoreconole; POE—Tetrahydride mouth furfuryl alcohol; POP—Butyl ether POP · POE butinoreethenore; tripolyoxypropylene glycerol etherol; POP-glycerin ether; POP-glycerin ether phosphate; POP · POE-pentane erythritol ether, polyglycerin and the like.
[0275] 油類としては、アボカド油、ォリーブ油、ゴマ油、ツバキ油、月見草油、タートル油、 マ力デミアンナッツ油、トウモロコシ油、ミンク油、ナタネ油、卵黄油、パーシック油、小 麦胚芽油、サザン力油、ヒマシ油、アマ二油、サフラワー油、綿実油、エノ油、大豆油 、落花生油、茶実油、カャ油、コメヌ力油、キリ油、ホホバ油、カカオ脂、ヤシ油、馬油 、パーム油、パーム核油、牛脂、羊脂、豚脂、ラノリン、鯨ロウ、ミツロウ、カルナウバロ ゥ、モクロウ、キャンデリラロゥ、スクヮラン等の動植物油及びその硬化油。流動バラフ イン、ワセリン等の鉱物油、トリパルミチン酸グリセリン等の合成トリグリセリンがある。  [0275] As oils, avocado oil, olive oil, sesame oil, camellia oil, evening primrose oil, turtle oil, madame demian nut oil, corn oil, mink oil, rapeseed oil, egg yolk oil, persic oil, oat germ oil , Southern power oil, castor oil, Amani oil, safflower oil, cottonseed oil, Eno oil, soybean oil, peanut oil, tea seed oil, cocoa oil, rice bran oil, kiri oil, jojoba oil, cocoa butter, coconut oil, Animal and vegetable oils such as horse oil, palm oil, palm kernel oil, beef tallow, sheep fat, pork tallow, lanolin, whale wax, beeswax, carnauba roux, moclaw, candelilla roux, squalane, and hardened oils thereof. There are fluidized paraffin, mineral oil such as petrolatum, and synthetic triglycerin such as glycerin tripalmitate.
[0276] 高級脂肪酸としては、例えばラウリン酸、ミリスチン酸、ノ ルミチン酸、ォレイン酸、リ ノール酸、リノレン酸、ステアリン酸、ベヘン酸、 12—ヒドロキシステアリン酸、イソステ アリン酸、ゥンデシン酸、トール酸、エイコサペンタエン酸、ドコサへキサェン酸などが ある。高級アルコールとしては、例えば、ラウリノレアノレコール、セチルアルコール、ステ ァリノレアノレ 一ノレ、ベへニノレアノレコーノレ、ミリスチノレアノレ: 3—ノレ、才レイノレアノレコーノレ 、セトステアリノレアノレコーノレ、ホホバァノレコーノレ、ラノリンァノレコーノレ、ノ チノレアノレコー ノレ、 2—デシノレテトラテセシノーノレ、コレステロ一ノレ、フィトステロ一ノレ、イソステアリノレ アルコール等がある。合成エステルとしては、例えば、オクタン酸セチル、ミリスチン酸 オタチルドデシル、ミリスチン酸イソプロピル、ミリスチン酸ミリスチル、パルミチン酸イソ プロピル、ステアリン酸プチル、ラウリン酸へキシル、オレンィ酸デシル、ジメチルォク タン酸、乳酸セチル、乳酸ミリスチル等がある。シリコーンとしては、例えば、ジメチル ポリシロキサン、メチノレフエニノレポリシロキサン等の鎖状ポリシロキサン、デカメチノレシ クロポリシロキサン等の環状ポリシロキサン、シリコーン樹脂等の三次元網目構造のも の等がある。  [0276] Examples of higher fatty acids include lauric acid, myristic acid, normitic acid, oleic acid, linoleic acid, linolenic acid, stearic acid, behenic acid, 12-hydroxystearic acid, isostearic acid, undecinic acid, and toric acid. Eicosapentaenoic acid and docosahexaenoic acid. Examples of the higher alcohol include laurino enorecol, cetyl alcohol, stearino enore monole, behenino enore enore, myristino enore: 3-nore, rei leino enore enore, ceto stearino enorenore, Jojobano Reconole, Lanolinano Reconole, Notino Reano Renore, 2-Desino Rete Tethecinore, Cholesterol Nore, Phytosterol Nore, Isostearinore Alcohol, etc. Synthetic esters include, for example, cetyl octanoate, octyldodecyl myristate, isopropyl myristate, myristyl myristate, isopropyl palmitate, butyl stearate, hexyl laurate, decyl orenate, dimethyl octanoate, cetyl lactate, Examples include myristyl lactate. Examples of the silicone include chain polysiloxanes such as dimethyl polysiloxane and methinorefinole polysiloxane, cyclic polysiloxanes such as decamethylenocyclopolysiloxane, and three-dimensional network structures such as silicone resins.
[0277] なお、発明を実施するための最良の形態の項においてなした具体的な実施態様お よび以下の実施例は、あくまでも、本発明の技術内容を明らかにするものであって、 そのような具体例にのみ限定して狭義に解釈されるべきものではなぐ当業者は、本 発明の精神および添付の特許請求の範囲内で変更して実施することができる。 実施例 [0277] It should be noted that the specific embodiments and the following examples made in the section of the best mode for carrying out the invention are intended to clarify the technical contents of the present invention to the last. Those skilled in the art, which should not be construed as narrowly limited to these specific examples, can make modifications within the spirit of the present invention and the scope of the appended claims. Example
[0278] 以下に第 1の発明の実施例を示して本発明を具体的に説明する力 本発明は実施 例に限定されるものではない。  [0278] The ability of the present invention to specifically describe the present invention with reference to the following examples of the first invention is not limited to the examples.
[0279] (実施例 1)力力ロールの製造 [0279] (Example 1) Production of force roll
〔実施例 1：モミジガサ抽出物の調製〕  [Example 1: Preparation of maple extract]
モミジガサ 2kgを凍結乾燥し、エタノール 4Lで抽出した。一次抽出物 16gに 60%ェ タノール 500mlを添加し、超音波処理 15分後、冷蔵庫でー晚静置した。 7000rpm で 15分間遠心分離し、沈殿物と分離した後の上清を濃縮し乾固物 8gを得た。得られ た抽出物をへキサン：酢酸ェチル = 20： 1を用いてシリカゲルカラムクロマトグラフィー で精製し、 HPLC純度 95%の力力ロールを得た。力力ロールの濃度は、以下に示す条 件で HPLC分析により測定した。この精製した力力ロールを、本発明の活性酸素消 去剤として用いた。  Momijigasa 2 kg was lyophilized and extracted with 4 L of ethanol. To 16 g of the primary extract, 500 ml of 60% ethanol was added, and after 15 minutes of sonication, the mixture was allowed to stand in a refrigerator. Centrifugation was performed at 7000 rpm for 15 minutes, and the supernatant after separation from the precipitate was concentrated to obtain 8 g of a dried product. The obtained extract was purified by silica gel column chromatography using hexane: ethyl acetate = 20: 1 to obtain a powerful roll having an HPLC purity of 95%. The strength roll concentration was measured by HPLC analysis under the following conditions. This purified force roll was used as the active oxygen scavenger of the present invention.
[0280] カラム： XterraMSC18、流速： lml/min、溶媒： 5%ァセトニトリル 20mMリン酸（ A)から 95%ァセトニトリル 20mMリン酸（B)へのグラジェント、検出：ダイオードアレイ デテクター 200— 700nm、カラム温度：室温。  [0280] Column: XterraMSC18, flow rate: lml / min, solvent: gradient from 5% acetonitrile 20 mM phosphoric acid (A) to 95% acetonitrile 20 mM phosphoric acid (B), detection: diode array detector 200—700 nm, column temperature :room temperature.
[0281] (実施例 2)スーパーォキシド消去能の測定  [0281] (Example 2) Measurement of superoxide erasing ability
スーパーォキシドデイスムターゼ（SOD)分析キット WST(Dojindo Molecular Tec hnologies社製）を用いて、力力ロールのスーパーォキシドの消去能について測定した 。具体的な方法を以下に記載する。なお、以下の方法で特に記載のない溶液等に ついては、上記測定キットに備わっているもの、或いは該キットの使用方法に従って 調整したものを使用した。  Superoxide dismutase (SOD) analysis kit WST (manufactured by Dojindo Molecular Technologies) was used to measure the superoxide elimination ability of the power roll. A specific method is described below. For the solutions not specifically described in the following method, those provided in the above measurement kit or those prepared according to the method of using the kit were used.
[0282] 96ウェルマイク口プレートに、各濃度の力力ロール溶液を各ゥエルに 20 μ L添加した 。次に、各ゥエルにテトラゾリゥム塩溶液を 200 L加え、よく混合した。その後、各ゥ エルに酵素溶液を 20 L添加し、直ちに 37°Cで 20分間インキュベートした後、マイク 口プレートリーダーで 450nmの吸光度を測定した。サンプルのスーパーォキシド消 去率は、式〔（B1— B3) (S-B2) ] / (B1-B3) X 100を計算することによって求めた。 ここで、 Bl (blankl)は酵素溶液添加 'MilliQ水添加での A を、 S (sample)は酵素溶  [0282] To a 96-well microphone opening plate, 20 μL of each strength roll solution was added to each well. Next, 200 L of tetrazolium salt solution was added to each well and mixed well. Thereafter, 20 L of the enzyme solution was added to each well and immediately incubated at 37 ° C for 20 minutes, and then the absorbance at 450 nm was measured with a microphone plate reader. The superoxide removal rate of the sample was determined by calculating the formula [(B1-B3) (S-B2)] / (B1-B3) X100. Here, Bl (blankl) is enzyme solution added 'A with MilliQ water addition, and S (sample) is enzyme solution added.
450  450
液添加 ·サンプル添加での A を、 B2 (Blank2)は酵素溶液無添加（代わりに酵素溶 液の希釈液を添加） ·サンプル添加での A を、 B3 (Blank3)は酵素溶液無添加（代わ りに酵素溶液の希釈液を添加） 'MilliQ水添加での A を示す。 Addition of liquid · A in sample addition, B2 (Blank2) no enzyme solution added (instead of enzyme solution) • Add A to the sample. B3 (Blank3) indicates no addition of enzyme solution (add dilution of enzyme solution instead).
[0283] 結果、力力ロールは終濃度 ImMで 28%のスーパーォキシド消去率を示した。一方、 比較品として用いたァスタキサンチン、ビタミン Eは同濃度では反応液中で析出する ため、評価できず、それ以下の析出しない濃度でもスーパーォキシド消去作用は見 られなかった。  [0283] As a result, the power roll showed a superoxide elimination rate of 28% at a final concentration of ImM. On the other hand, astaxanthin and vitamin E used as comparative products were precipitated in the reaction solution at the same concentration, and thus could not be evaluated. Superoxide scavenging action was not observed even at concentrations below that level.
[0284] (実施例 3)過酸化水素消去能の測定  [0284] (Example 3) Measurement of hydrogen peroxide scavenging ability
0.2Mリン酸緩衝液 (ρΗ6·0) 500 1、 13mM 4-ァミノアンチピリン 100 1、 9.6mM T OOSIOO^ U lOU/mlペルォキシダーゼ  0.2 M phosphate buffer (ρΗ6.0) 500 1, 13 mM 4-aminominopyrine 100 1, 9.6 mM T OOSIOO ^ U lOU / ml peroxidase
100 1を混和した試薬液を 37°Cでプレインキュペートした。 ImM過酸化水素 100 1と 各濃度の力力ロール溶液 100 1を混和し、 37°Cで 20分間インキュベートし、直ちに 上記の試薬液と混和し、 37°Cで 5分間反応させた。マイクロプレートリーダーで 555nm の吸光度を測定した。なお、コントロールとして、下記の条件についても同様の方法 で測定した。  The reagent solution mixed with 100 1 was preincubated at 37 ° C. ImM hydrogen peroxide 100 1 and power roll solution 100 1 of each concentration were mixed, incubated at 37 ° C for 20 minutes, immediately mixed with the above reagent solution, and reacted at 37 ° C for 5 minutes. Absorbance at 555 nm was measured with a microplate reader. As a control, the following conditions were also measured in the same manner.
サンプルの過酸化水素消去率は、式 100_ (S— B2) /B1 X 100を計算することによって 求めた。  The hydrogen peroxide scavenging rate of the sample was obtained by calculating the formula 100_ (S—B2) / B1 × 100.
ここで、 S (sample)は過酸化水素添加'サンプル添加での A を、 Bl (Blank 1)は過酸 化水素添加 'MilliQ水添加での A を、 B2 (Blank2)は過酸化水素無添加（代わりに M illiQ水添加）'サンプル添加での A を示す。  Here, S (sample) is A with hydrogen peroxide added 'sample added, Bl (Blank 1) is hydrogenated with hydrogen peroxide' A with MilliQ water added, B2 (Blank 2) is not added with hydrogen peroxide (Instead, MilliQ water added) 'Shows A for sample addition.
[0285] 比較対象物質として、ァスタキサンチンを用いた。結果を図 1に示す。力力ロールに はァスタキサンチンと比較して高い過酸化水素消去率が見られた。一方、比較品とし て用いたビタミン Eは同濃度では反応液中で析出するため、評価できず、それ以下 の析出しない濃度でもスーパーォキシド消去作用は見られなかった。  [0285] Astaxanthin was used as a substance to be compared. The results are shown in Figure 1. The power roll showed a high hydrogen peroxide scavenging rate compared to wastaxanthin. On the other hand, Vitamin E used as a comparative product was precipitated in the reaction solution at the same concentration, so it could not be evaluated, and no superoxide scavenging action was observed even at concentrations below that.
[0286] (実施例 4)一重項酸素消去能の測定  [0286] (Example 4) Measurement of singlet oxygen scavenging ability
一重項酸素消去能の測定は文献（Biotechnology Letters., 21， 265 (1999))に記載 の方法に準じて実施した。  Singlet oxygen scavenging ability was measured according to the method described in the literature (Biotechnology Letters., 21, 265 (1999)).
[0287] 0.05mMメチレンブルー溶液 100 1、 2.4Mリノ一ノレ酸 25 1、力力ロール溶液 100  [0287] 0.05 mM methylene blue solution 100 1, 2.4 M linolenic acid 25 1, force roll solution 100
H 1、エタノール 775 H 1を混和し、 25°C、 7，0001uxの条件下で 3h反応させた。反応後、 反応液を 30倍に希釈し、 Abs235を測定した。なお、コントロールとして、下記の条件 につ!/、ても同様の方法で測定した。 H 1 and ethanol 775 H 1 were mixed and reacted at 25 ° C. and 7,0001 ux for 3 hours. After the reaction The reaction solution was diluted 30 times and Abs235 was measured. As a control, the same conditions were used for the following conditions!
サンプルの一重項酸素消去率は、式 100_ (S— B2) /B1 X 100を計算することによって 求めた。  The singlet oxygen scavenging rate of the sample was obtained by calculating the formula 100_ (S—B2) / B1 × 100.
ここで、 S (sample)は光照射 ·サンプル添加での A を、 Bl (Blank 1)は光照射 'MilliQ 水添加での A を、 B2 (Blank2)は光無照射'サンプル添加での A を示す。  Where S (sample) is A with light irradiation and sample addition, Bl (Blank 1) is A with light irradiation 'MilliQ water addition, and B2 (Blank2) is A with light non-irradiation sample addition. Show.
[0288] 比較対象物質として、ァスタキサンチン、ビタミン Eを用いた。結果を図 2に示す。力力 ロールは、一重項酸素消去剤として知られるァスタキサンチンと比較すると消去率は 低いが、ビタミン Eよりも高い一重項酸素消去能を有することが示された。  [0288] Astaxanthin and vitamin E were used as comparative substances. The result is shown in figure 2. It was shown that the power roll has a higher singlet oxygen scavenging ability than vitamin E, although the scavenging rate is lower than that of wastaxanthin, which is known as a singlet oxygen scavenger.
[0289] (実施例 5)安定性試験  [Example 5] Stability test
力力ロールを 0. 5%濃度でブチレングリコールに溶解し、室温'明所、室温'喑所、 4°C .喑所に 3ヶ月間静置し、力力ロールの安定性を実施例 1記載の HPLC条件で解 析した。結果を図 3に示す。力力ロールはいずれの条件でも高い安定性を示し、本発 明の活性酸素消去剤は長期にわたりその効果を維持することがわかった。  Example 1 The strength roll was dissolved in butylene glycol at a concentration of 0.5% and allowed to stand at room temperature 'light place, room temperature' place, 4 ° C. Analysis was performed under the HPLC conditions described. The results are shown in Figure 3. It was found that the power roll showed high stability under any condition, and the active oxygen scavenger of the present invention maintained its effect for a long time.
[0290] (実施例 6)美容液の製造  [Example 6] Manufacture of serum
以下に示す組成の美容液を常法により製造した。コントロールとして、力力ロールを 含まない美容液も常法により製造した。  A cosmetic liquid having the following composition was produced by a conventional method. As a control, a serum containing no force roll was also produced by a conventional method.
[0291] (組成） （重量％)  [0291] (Composition) (wt%)
ソノレビット 4. 0  Sonorebit 4.0
ジプロピレングリコール 6. 0  Dipropylene glycol 6.0
ポジエチレングリコーノレ 1500 5. 0  Positive ethylene glycolate 1500 5. 0
POE (20)ォレイルアルコールエーテル 0. 5  POE (20) oleyl alcohol ether 0.5
ショ糖脂肪酸エステル 0. 2  Sucrose fatty acid ester 0.2
メチノレセノレロース 0. 2  Methinoresenorelose 0.2
力力ロール（力力ロール濃度 0.01%) 1. 0  Power roll (power roll concentration 0.01%) 1.0
精製水 全体で 100となる量  Total amount of purified water is 100
[0292] (実施例 7)乳液の製造  [0292] (Example 7) Production of emulsion
以下に示す組成の乳液を常法により製造した。コントロールとして、力力ロールを ない乳液も常法により製造した。 An emulsion having the composition shown below was produced by a conventional method. Power roll as a control No emulsions were prepared by conventional methods.
[0293] (組成） （重量％) [0293] (Composition) (wt%)
グリセリノレエーテノレ 1. 5  Griselinoleetenore 1.5
ショ糖脂肪酸エステル 1. 5  Sucrose fatty acid ester 1.5
1. 0  Ten
7. 5  7.5
ジプロピレングリコール 5. 0  Dipropylene glycol 5.0
力力ロール（力力ロール濃度 0.01%) 1. 0  Power roll (power roll concentration 0.01%) 1.0
精製水 全体で 100となる量  Total amount of purified water is 100
[0294] (実施例 8)クリームの製造  [Example 8] Production of cream
以下に示す組成のクリームを常法により製造した。コントロールとして、力力ロールを 含まないクリームも常法により製造した。  A cream having the following composition was produced by a conventional method. As a control, a cream containing no force roll was also produced by a conventional method.
[0295] (組成） [0295] (Composition)
プロピレングリコーノレ 6. 0  Propylene glycolate 6.0
フタル酸、： 19. 0  Phthalic acid, 19.0
5. 0  5. 0
5. 0  5. 0
12. 0  12. 0
酸ポリエ： 38. 0  Acid polyester: 38.0
ェデト酸ナトリウム 0. 03  Sodium edetate 0.03
力力ロール（力力ロール濃度 0.01%) 1. 0  Power roll (power roll concentration 0.01%) 1.0
精製水 全体で 100となる  Purified water total 100
[0296] (実施例 9)官能評価  [Example 9] Sensory evaluation
(試験方法）  (Test method)
実施例 6〜8を用いて官能評価を行った。なお、力力ロールを含まない比較例も同 時に評価した。官能評価は、 22〜45才の 10人をパネルとして、 1日 2回， 3力月間に わたって適量を洗顔後に塗布した。塗布による美肌及び皮膚老化防止効果を下記 の基準により評価した。 [0297] 美肌効果： Sensory evaluation was performed using Examples 6-8. A comparative example not including a force roll was also evaluated at the same time. For sensory evaluation, 10 persons aged 22 to 45 years were applied as panels, and an appropriate amount was applied twice a day for 3 months after face washing. The effects of beautification and skin aging prevention by application were evaluated according to the following criteria. [0297] Skin softening effect:
〔評価〕 〔内容〕  [Evaluation] [Contents]
有効肌のくすみが目立たなくなった  Effective skin dullness is not noticeable
やや有効肌のくすみがあまり目立たなくなった  Slightly less effective skin dullness
無効使用前後で変化なし  No change before and after invalid use
[0298] 皮膚老化防止効果： [0298] Skin aging prevention effect:
〔評価〕 〔内容〕  [Evaluation] [Contents]
有効肌のはり、つやが改善された  Effective skin elasticity and gloss improved
やや有効肌のはり、つやが少し改善された  Slightly improved skin elasticity and gloss
無効使用前後で変化なし  No change before and after invalid use
[0299] 官能評価の結果として、各項目における評価者の数を表 1に示した。表 1の結果に 示される如ぐ本発明品の活性酸素消去剤を皮膚に適用することにより、肌のくすみ 等を押え、美しい肌とするとともに、肌のはり、つやが改善され、皮膚の老化を防止す ることが明力、となった。 [0299] As a result of sensory evaluation, the number of evaluators in each item is shown in Table 1. By applying the active oxygen scavenger of the product of the present invention as shown in the results of Table 1 to the skin, skin dullness, etc. can be suppressed to make the skin beautiful and the skin tone and gloss improved, and the skin aging It became clear that preventing this.
[0300] [表 1] [0300] [Table 1]
Figure imgf000066_0001
Figure imgf000066_0001
[0301] 力力ロールを含まない比較例より実施例の方力 8割以上のパネルが皮膚の弾力性 が回復し、シヮの軽減が認められたと回答しており、顕著な皮膚の老化症状の回復 効果がある [0301] More than 80% of the panel of the example compared to the comparative example that did not include a force roll, the skin elasticity was recovered and the wrinkles were relieved. Has a recovery effect
ことが示された。  It was shown that.
[0302] 以下に第 2の発明の実施例を示して本発明をより具体的に説明するが、これらは単 なる例示であって、本発明の範囲を何ら限定するものではない。  [0302] Hereinafter, the present invention will be described more specifically with reference to examples of the second invention. However, these are merely examples, and do not limit the scope of the present invention.
[0303] 〔実施例 10 :モミジガサ抽出物の調製〕 モミジガサ 250gを凍結乾燥し、塩化メチレンとメタノール 1： 1の混液 500mlで抽出 した。得られた抽出物をへキサン：酢酸ェチル = 20 : 1を用いてシリカゲルカラムクロ マトグラフィ一で精製し、力力ロール画分を得た。力力ロールの濃度は、以下に示す 条件で HPLC分析により測定した。 [0303] [Example 10: Preparation of maple extract] 250 g of Momijigasa was lyophilized and extracted with 500 ml of a mixture of methylene chloride and methanol 1: 1. The obtained extract was purified by silica gel column chromatography using hexane: ethyl acetate = 20: 1 to obtain a force roll fraction. The concentration of the force roll was measured by HPLC analysis under the following conditions.
[0304] カラム： XterraMSC18、流速： lml/min、溶媒： 5%ァセトニトリル 20mMリン酸（ A)から 95%ァセトニトリル 20mMリン酸（B)へのグラジェント、検出：ダイオードアレイ デテクター 200— 700nm、カラム温度：室温。  [0304] Column: XterraMSC18, flow rate: lml / min, solvent: gradient from 5% acetonitrile 20 mM phosphoric acid (A) to 95% acetonitrile 20 mM phosphoric acid (B), detection: diode array detector 200—700 nm, column temperature :room temperature.
[0305] 〔実施例 11：正常ヒト皮膚繊維芽細胞を用いた細胞賦活作用の評価〕  [Example 11: Evaluation of cell activation using normal human dermal fibroblasts]
正常ヒト皮膚繊維芽細胞トータルキット（CA106K05、製造元：セルアプリケイショ ンズインク USA、輸入販売元：東洋紡績株式会社)を用いて、常法により正常ヒト皮 膚繊維芽細胞を培養した。  Normal human skin fibroblasts were cultured by a conventional method using a normal human skin fibroblast total kit (CA106K05, manufacturer: Cell Applications Inc. USA, importer / distributor: Toyobo Co., Ltd.).
[0306] 正常ヒト皮膚繊維芽細胞を 1ゥエル当たり 2· 0 X 104個となるように 48穴マイクロプ レートに播種した。播種培地には、ダルベッコ改変イーグル培地（DMEM)に 10% のゥシ胎児血清を添加したものを用いた。 37°C、二酸化炭素濃度 5vol%中にて 24 時間培養後、力力ロールの終濃度が 0. 001ng/ml〜； 100000ng/mlとなるように モミジガサ抽出物を添加した試験培地に交換し、さらに 48時間培養した。なお、モミ ジガサ抽出物はエタノールに溶解後、エタノールで段階希釈し、エタノールの終濃度 がすべて 0. 5%となるように各培地に添加した。溶媒対照として、エタノール群（終濃 度 0. 5%)を設けた。また、細胞毒性物質により細胞増殖が阻害されることを確認す るために SDS添加群（終濃度 0· 1 %)を設けた。次いで 3—（4, 5—ジメチルー 2— チアゾリル） - 2, 5—ジフエニルテトラゾリゥムブロミド（MTT)を 100 μ g/mL含有す る培地に交換して 3時間培養し、テトラゾリゥム環の開環により生じるフオルマザンを 2 —プロパノールにて抽出し、マイクロプレートリーダーにて 550nmの吸光度を測定し た。同時に濁度として 650nmにおける吸光度を測定し、両測定値の差により細胞賦 活作用を評価した。 [0306] Normal human dermal fibroblasts were seeded in a 48-well microplate so that there were 2 · 0 × 10 4 per well. The seeding medium used was Dulbecco's modified Eagle medium (DMEM) supplemented with 10% urchin fetal serum. After culturing at 37 ° C in a carbon dioxide concentration of 5 vol% for 24 hours, replace the test roll with Momijigasa extract so that the final concentration of the power roll is 0.001 ng / ml to 100000 ng / ml. The cells were further cultured for 48 hours. The maple extract was dissolved in ethanol, serially diluted with ethanol, and added to each medium so that the final concentration of ethanol was 0.5%. As a solvent control, an ethanol group (final concentration 0.5%) was provided. In addition, an SDS-added group (final concentration: 0.1%) was provided to confirm that cell growth was inhibited by cytotoxic substances. Next, the medium was replaced with a medium containing 100 μg / mL of 3- (4,5-dimethyl-2-thiazolyl) -2,5-diphenyltetrazolium bromide (MTT) and cultured for 3 hours. Formazan produced by ring-opening was extracted with 2-propanol and the absorbance at 550 nm was measured with a microplate reader. At the same time, the absorbance at 650 nm was measured as turbidity, and the cell activation effect was evaluated by the difference between the two measured values.
[0307] 評価結果を、エタノール群 (溶媒対照群）における細胞賦活作用を 100とした相対 ィ直にて図 4に示した。  [0307] The evaluation results are shown in Fig. 4 as a relative straightness with the cell activation effect in the ethanol group (solvent control group) taken as 100.
[0308] 図 4から明らかなように、モミジガサ抽出物添加群では、いずれの濃度においても 正常ヒト皮膚繊維芽細胞に対してエタノール群より高い細胞賦活作用を示した。特に 、力力ロールの終濃度が lng/ml、 0. Ing/mlとなるようにモミジガサ抽出物を添加 した場合には、エタノール群と比較して、有意に高い細胞賦活作用が見られた。なか でも lng/ml群は、 80%以上の有意な細胞賦活作用が認められた。さらに高濃度を 添加した場合には、細胞賦活作用は低下したが細胞毒性は全く見られな力 た。一 方、 1 %のソディウムドデシルサルフェート（SDS)を添加した群では、細胞毒性により 細胞の生存率が 20%に低下した。 [0308] As is apparent from Fig. 4, in the maple extract added group, at any concentration. It showed higher cell activation than normal ethanol on normal human skin fibroblasts. In particular, when Momijigasa extract was added so that the final concentration of the power roll was 1 ng / ml and 0. Ing / ml, a significantly higher cell activating effect was seen compared to the ethanol group. In particular, the lng / ml group showed a significant cell activation effect of 80% or more. When a higher concentration was added, the cell activation effect was reduced, but no cytotoxicity was observed. On the other hand, in the group supplemented with 1% sodium dodecyl sulfate (SDS), cell viability decreased to 20% due to cytotoxicity.
[0309] この結果から、モミジガサ抽出物は、優れた細胞賦活作用を有することが明ら力、とな り、モミジガサ抽出物を肌に適用することにより、極めて優れた老化防止効果を発揮 し、加齢、紫外線曝露等により生じる皮膚のしわ、たるみ等を効果的に改善できること が示唆された。 [0309] From this result, it can be clearly seen that the maple extract has an excellent cell activation effect, and by applying the maple extract to the skin, it exhibits an extremely excellent anti-aging effect, It was suggested that skin wrinkles and sagging caused by aging, UV exposure, etc. can be effectively improved.
[0310] 〔実施例 12：美容液の製造〕 [0310] [Example 12: Production of serum]
以下に示す組成の美容液を常法により製造した。コントロールとして、モミジガサ柚 出物を含まない美容液も常法により製造した。  A cosmetic liquid having the following composition was produced by a conventional method. As a control, a serum containing no Momijigasa extract was also produced by a conventional method.
(組成） （重量％)  (Composition) (wt%)
4. 0  4. 0
ジプロピレングリコーノレ 6. 0  Dipropylene glycol 6.0
ポリエチレングリコーノレ 1500 5. 0  Polyethylene glycol nore 1500 5. 0
POE (20)ォレイルアルコールエーテル 0. 5  POE (20) oleyl alcohol ether 0.5
ショ糖脂肪酸： 0. 2  Sucrose fatty acids: 0.2
メチノレセノレロース 0. 2  Methinoresenorelose 0.2
モミジガサ抽出物 0. 1  Maple extract 0.1
精製水 全体で 100となる量  Total amount of purified water is 100
〔実施例 13 :乳液の製造〕  Example 13: Production of emulsion
以下に示す組成の乳液を常法により製造した。コントロールとして、モミジガサ抽出 物を含まない乳液も常法により製造した。  An emulsion having the composition shown below was produced by a conventional method. As a control, an emulsion containing no Momijigasa extract was also produced by a conventional method.
(組成） （重量％)  (Composition) (wt%)
エーテノレ 1. 5 ショ糖脂肪酸エステル 1. 5 Etenore 1.5 Sucrose fatty acid ester 1.5
1. 0  Ten
7. 5  7.5
ジプロピレングリコール 5. 0  Dipropylene glycol 5.0
モミジガサ抽出物 0. 1  Maple extract 0.1
精製水 全体で 100となる量  Total amount of purified water is 100
〔実施例 14 :クリームの製造〕  Example 14: Production of cream
以下に示す組成のクリームを常法により製造した。コントロールとして、モミジガサ柚 出物を含まないクリームも常法により製造した。  A cream having the following composition was produced by a conventional method. As a control, a cream containing no Momijigasa extract was also produced by a conventional method.
(組成） (重量％)  (Composition) (wt%)
プロピレングリコ 6. 0  Propylene glycol 6.0
フタル酸、： 19. 0  Phthalic acid, 19.0
5. 0  5. 0
5. 0  5. 0
12. 0  12. 0
酸ポリエ： 38. 0 ェデト酸ナトリウム 0. 03  Acid polyester: 38.0 sodium edetate 0.03
モミジガサ抽出物 0. 1  Maple extract 0.1
精製水 全体で 100となる量  Total amount of purified water is 100
〔実施例 15 :使用試験〕  (Example 15: Use test)
上記実施例 14で製造したクリームを用いて使用試験を実施した。評価項目はシミ、 クスミについて改善効果を自己評価した。比較例として、モミジガサ抽出物を配合し ないクリームをブラインドで使用した。本クリームを用いて 25歳〜 50歳のパネラー 5名 に 1ヶ月使用してもらい、使用前後の評価を、改善した、やや改善した、変化なしの 三段階で評価した。  A use test was conducted using the cream produced in Example 14 above. The evaluation items were self-evaluation of the improvement effect on spots and kusumi. As a comparative example, a cream containing no Momijigasa extract was used blind. Using this cream, five panelists aged 25 to 50 years used it for 1 month, and evaluated before and after use in three stages: improved, slightly improved, and no change.
[0311] 結果を表 2に示した。表 2より明らかなように、モミジガサ抽出物を配合したクリーム を使用した場合、たるみ、くすみといつた皮膚老化に関する評価に改善が見られた。  [0311] The results are shown in Table 2. As can be seen from Table 2, when the cream containing Momijigasa extract was used, there was an improvement in the evaluation of sagging, dullness, and when skin aging occurred.
[0312] [表 2] 比較例 実施例 改香 2 [0312] [Table 2] Comparative Example Example Aroma 2
やや改 1 2 変化無し 4 1 計 5 5  Slightly modified 1 2 No change 4 1 Total 5 5
[0313] 次に、上記処方を用いて使用試験を行い、シヮ，肌荒れについて改善効果を評価し た。比較例として、モミジガサ抽出物を配合しないクリームをブラインドで使用した。本 クリームを用いて 25歳〜 50歳のパネラー 10名に 1ヶ月使用してもらい、使用前後の 評価を、皮膚症状の指標として、シヮ，肌荒れについて、「改善」， 「やや改善」， 「変 化なし」の三段階で評価し、表 3に結果を示した。 [0313] Next, a use test was conducted using the above-mentioned formulation, and the effect of improving wrinkles and rough skin was evaluated. As a comparative example, a cream containing no Momijigasa extract was used blind. Using this cream, 10 panelists between the ages of 25 and 50 used it for 1 month, and the evaluation before and after use was used as an index of skin symptom, with “improved”, “slightly improved”, “ The results were evaluated in three stages, “No change”, and the results are shown in Table 3.
[0314] [表 3]  [0314] [Table 3]
Figure imgf000070_0001
Figure imgf000070_0001
[0315] 表 3より、シヮ，肌荒れについて、モミジガサ抽出物を含有しない比較例使用群におい ては、 7割以上のパネラーに改善は認められな力、つた力 モミジガサ抽出物を配合し た実施例使用群においては、 7割以上のパネラーに改善が認められた。 [0315] From Table 3, for wrinkles and rough skin, in the comparative use group that does not contain Momijigasa extract, more than 70% of panelists showed improvement in power and strength. In the example use group, more than 70% of panelists improved.
[0316] 以上のように、本発明の実施例においては、従来の比較例よりも、シヮ、及び肌荒の 改善に優れた効果を有してレ、た。  [0316] As described above, the examples of the present invention were more effective in improving wrinkles and rough skin than the conventional comparative examples.
[0317] 以下に第 3および第 4の発明の実施例を示して本発明をより具体的に説明するが、 これらは単なる例示であって、本発明の範囲を何ら限定するものではない。  [0317] Hereinafter, the present invention will be described more specifically with reference to Examples of the third and fourth inventions. However, these are merely examples, and do not limit the scope of the present invention.
[0318] 〔実施例 15：含水率の異なるエタノールを用レ、た力カロール抽出物の調製〕 モミジガサの葉 2Kgを凍結乾燥し、ミキサーで撹拌して粉末 212gを得た。この粉末 にエタノール 4Lを加え 12時間撹拌し、濾過して不溶物を除去後、濃縮し緑色の一 次抽出物 16gを得た。この抽出物 0. lgに水あるいは 10%〜90%の水を含むエタノ ールをそれぞれ 8ml加え、超音波処理後、 5°Cでー晚静置した。 7000rpmで 15分 間遠心分離し、上清と沈殿物に分けた。上清はそのまま、沈殿物はジメチルホルムァ ミド 8mlに溶解して、力力ロール測定用サンプルとした。 [0318] [Example 15: Preparation of tartorol extract using ethanol with different water contents] 2 kg of Momijigasa leaves were lyophilized and stirred with a mixer to obtain 212 g of powder. 4 L of ethanol was added to this powder, stirred for 12 hours, filtered to remove insoluble matters, and concentrated to obtain 16 g of a green primary extract. 8 ml each of water or ethanol containing 10% to 90% water was added to 0. lg of this extract, and after sonication, the mixture was allowed to stand at 5 ° C. Centrifugation was performed at 7000 rpm for 15 minutes to separate the supernatant and the precipitate. The supernatant was used as it was, and the precipitate was dissolved in 8 ml of dimethylformamide to prepare a sample for measuring force rolls.
[0319] サンプル中の力力ロールの濃度は、以下に示す条件で HPLC分析により測定した 。カラム： XterraMSC18、流速： lml/min、溶媒： 5%ァセトニトリル 20mMリン酸（ A)から 95%ァセトニトリル 20mMリン酸（B)へのグラジェント、検出：ダイオードアレイ デテクター 200— 700nm、カラム温度：室温、インジェクション量： 20〃 1。  [0319] The concentration of the power roll in the sample was measured by HPLC analysis under the following conditions. Column: XterraMSC18, flow rate: lml / min, solvent: gradient from 5% acetonitrile 20 mM phosphoric acid (A) to 95% acetonitrile 20 mM phosphoric acid (B), detection: diode array detector 200-700 nm, column temperature: room temperature, Injection volume: 20〃1.
[0320] 沈殿物中の力力ロール濃度の結果を図 5に、上清中の力力ロール濃度の結果を図  [0320] Fig. 5 shows the results of the power roll concentration in the sediment, and Fig. 5 shows the results of the power roll concentration in the supernatant.
6に示した。図 5および図 6から分かるように、エタノール濃度 50%〜90%において は、力力ロールは上清から回収され、 0%〜30%においては沈殿物として回収された 。このこと力、ら、 50%〜； 100%エタノール水溶液で処理することにより、極性が低いク ロロフィル等と力カロールを分離でき、 0 %〜 30 %エタノール水溶液で処理することに より極性が高い物質と分離できることが明らかとなった。  Shown in 6. As can be seen from FIG. 5 and FIG. 6, the force roll was recovered from the supernatant at an ethanol concentration of 50% to 90%, and was recovered as a precipitate at 0% to 30%. This force, et al., 50% or more; By treating with 100% ethanol aqueous solution, chlorophyll and the like having low polarity can be separated from force carol, and by treating with 0% to 30% ethanol aqueous solution, a substance having high polarity It became clear that it can be separated.
[0321] 〔実施例 16 60%エタノールと 20%エタノールを用いた力力ロールを含む植物抽 出物の製造〕  [Example 16: Production of plant extract containing force roll using 60% ethanol and 20% ethanol]
実施例 15と同様の方法で抽出した一次抽出物 16gに 60%エタノール 500mlを添 加し、超音波処理 15分後、冷蔵庫でー晚静置した。 7000rpmで 15分間遠心分離し 、沈殿物と分離した後の上清を濃縮し乾固物 8gを得た。上清および沈殿物中の力力 ロール含量とクロロフィル含量を測定したところ上清中には力力ロール 1132mg、クロ 口フィル 3mg、沈殿物中には力力ロール 294mg、クロロフィノレ 718mgとなり、上清を 回収して濃縮乾固することで、低クロロフィル含量かつ高力力ロール含量のモミジガ サ抽出物を得られることが分力、つた。  To 16 g of the primary extract extracted in the same manner as in Example 15, 500 ml of 60% ethanol was added, and after 15 minutes of sonication, the mixture was allowed to stand in a refrigerator. Centrifugation was carried out at 7000 rpm for 15 minutes, and the supernatant after separation from the precipitate was concentrated to obtain 8 g of a dried product. The force roll content and chlorophyll content in the supernatant and the precipitate were measured. The supernatant was 1132 mg power roll and 3 mg black mouth fill, and 294 mg power roll and 718 mg chlorofinole in the precipitate. By collecting and concentrating to dryness, it was possible to obtain a maple extract with a low chlorophyll content and a high strength roll content.
[0322] 次いで、得られた 60%エタノール抽出上清の乾固物に 20%エタノール 500mlを添 加し、超音波処理後、冷蔵庫でー晚静置した。 7000rpmで 15分間遠心分離し、沈 殿物 1. 5gを得た。上清および沈殿物中の力力ロール含量とクロロフィル含量を測定 したところ上清中には力力ロール 67mg、クロロフィル 0. lmg、沈殿物中には力カロ 一ノレ 728mg、クロロフィル 3mgとなり、力力ロールより高親水性の夾雑物（極性物質） を除去した力力ロール抽出物を得ることができた。 [0322] Next, 500 ml of 20% ethanol was added to the dried solid of the obtained 60% ethanol extraction supernatant, and after sonication, it was allowed to stand in a refrigerator. Centrifugation was performed at 7000 rpm for 15 minutes to obtain 1.5 g of a sediment. Measure power roll content and chlorophyll content in supernatant and sediment As a result, in the supernatant, power roll 67 mg, chlorophyll 0.1 mg, and in the precipitate power caro monore 728 mg, chlorophyll 3 mg, which removes highly hydrophilic impurities (polar substances) from the power roll. A force roll extract could be obtained.
[0323] なお、本実施例 16のスキームを図 7に示した。 [0323] The scheme of Example 16 is shown in FIG.
[0324] 力力ロール濃度は、実施例 15に記載の方法で測定した。クロロフィルの濃度は、抽 出物をアセトンに溶解し、 660nmと 642. 5nmの吸光度を測定することにより、全クロ ロフィノレ量（mg/l) = 7. 12D + D に従って算出した。  [0324] The force roll concentration was measured by the method described in Example 15. The concentration of chlorophyll was calculated according to the total amount of chlorofinole (mg / l) = 7.12D + D by dissolving the extract in acetone and measuring the absorbance at 660 nm and 642.5 nm.
660 642. 5  660 642. 5
[0325] 〔実施例 17 力力ロールを含む植物抽出物によるコラーゲン産生能の評価〕  [Example 17: Evaluation of collagen production ability by plant extract containing powerful roll]
力カロールを含む植物抽出物 (試料）は実施例 16で製造された植物抽出物を用レ、 た。評価は、以下の手順で行った。  The plant extract (sample) containing force carol was the plant extract produced in Example 16. The evaluation was performed according to the following procedure.
[0326] 正常ヒト皮膚繊維芽細胞トータルキット（CA106K05、製造元：セルアプリケイショ ンズインク USA、輸入販売元：東洋紡績株式会社)を用いて、常法により正常ヒト皮 膚繊維芽細胞を培養した。  [0326] Normal human dermal fibroblasts were cultured by a conventional method using a normal human dermal fibroblast total kit (CA106K05, manufacturer: Cell Applications Inc. USA, import sales company: Toyobo Co., Ltd.).
[0327] 正常ヒト皮膚線維芽細胞を 1ゥエル当たり 1 · 0 X 105個となるように 48穴マイクロプ レートに播種した。播種培地には、ダルベッコ改変イーグル培地（DMEM)に 1 %の ゥシ胎児血清を添加したものを用いた。 37°C、二酸化炭素濃度 5vol%中にて 24時 間培養後、 PBS (—）で 2回洗浄した後、力力ロール濃度 0、 0. 01および 0. lng/m 1の試料を添加した無血清培地に交換し、さらに 50時間同条件にて培養した。またブ ランクとして、エタノールを同一容量添加した無血清培地に交換し、さらに 50時間同 条件にて培養した。培養上清から、ヒト線維芽細胞が産生する I型プロコラーゲン C末 ヘプナ卜 (Procollagen typel carboxyterminal propeptide： PIP)を、 Procollagen typ e [0327] Normal human dermal fibroblasts were seeded in a 48-well microplate at 1 · 0 × 10 5 per well. The seeding medium used was Dulbecco's modified Eagle medium (DMEM) supplemented with 1% urchin fetal serum. After 24 hours of incubation at 37 ° C and carbon dioxide concentration of 5 vol%, after washing twice with PBS (—), samples with force roll concentrations of 0, 0.01 and 0.1 ng / m 1 were added. The serum-free medium was replaced, and the cells were further cultured under the same conditions for 50 hours. In addition, as a blank, the medium was replaced with a serum-free medium supplemented with the same volume of ethanol, and further cultured under the same conditions for 50 hours. Procollagen typel carboxyterminal propeptide (PIP) produced by human fibroblasts from the culture supernatant
I C-peptide (PIP) EIA Kit (TaKaRa)で測定した。コラーゲン産生促進率は、標準品を 上記 ELISAキットにて測定し、その結果から検量線を作成、その検量線から試料添 加時のコラーゲン産生量および試料無添加時のコラーゲン産生量を求めた。  It was measured with IC-peptide (PIP) EIA Kit (TaKaRa). The collagen production acceleration rate was determined by measuring a standard product with the above-mentioned ELISA kit, creating a calibration curve from the results, and determining the collagen production amount when the sample was added and the collagen production amount when no sample was added from the calibration curve.
[0328] 結果を図 8に示した。横軸は抽出物中の力力ロール濃度を示す。図 8から明らかな ように、力力ロールを含む植物（モミジガサ）抽出物を添加した培地では、ヒト皮膚のコ ラーゲン産生活性（コラーゲン産生促進作用）がブランク（エタノール）に比べて顕著 に高まることが認められた。この結果から、力力ロールを含む植物抽出物を適用する ことにより、加齢に伴う繊維芽細胞の衰えが軽減し、コラーゲンの産生を促進してコラ 一ゲン量を維持することができる。その結果、加齢、紫外線曝露等により生じる皮膚 のしわ、たるみ等を効果的に改善することができる。 The results are shown in FIG. The horizontal axis represents the force roll concentration in the extract. As is clear from FIG. 8, the collagen production activity (collagen production promoting effect) of human skin is more remarkable than that of the blank (ethanol) in the medium supplemented with the extract of plant (Momigasa) containing a powerful roll. It was recognized that it would increase. From this result, by applying a plant extract containing a power roll, the decline of fibroblasts with aging can be reduced, collagen production can be promoted, and the amount of collagen can be maintained. As a result, it is possible to effectively improve skin wrinkles and sagging caused by aging and exposure to ultraviolet rays.
産業上の利用可能性  Industrial applicability
[0329] 本発明の第 1の発明により、植物由来のラジカル消去作用、脂質過酸化抑制作用 等の優れた活性酸素消去剤を提供することができる。また、本発明の第 1の発明は、 植物抽出物の活性酸素消去活性の有効成分を同定しており、より純化された物質と して取得することで、より安全で有効性の安定した生理活性物質として、化粧品、機 能性食品、医薬品等に幅広く利用することを目的とする。本発明の第 1の発明の活 性酸素消去剤は微量で強!/、活性酸素消去効果を有しており、該活性酸素消去剤を 飲食物、医薬品、化粧品、美白剤、肥料、飼料や皮膚外用剤等に使用することにより 、抗酸化効果、特には製品の保存安定性の向上効果等の優れた酸化防止効果や、 各種疾病予防効果ゃ抗酸化効果等を得ることができる。従って、産業界に大きく寄 与することが期待される。  [0329] According to the first invention of the present invention, it is possible to provide an excellent active oxygen scavenger such as a plant-derived radical scavenging action and a lipid peroxidation inhibiting action. In addition, the first invention of the present invention identifies an active ingredient of the active oxygen scavenging activity of a plant extract. By obtaining it as a more purified substance, it is possible to obtain a safer and more stable physiological effect. It is intended to be widely used as an active substance in cosmetics, functional foods, pharmaceuticals, etc. The active oxygen scavenger of the first invention of the present invention is strong in a small amount! /, And has an active oxygen scavenging effect. The active oxygen scavenger is used for foods and drinks, pharmaceuticals, cosmetics, whitening agents, fertilizers, feeds and the like. By using it as an external preparation for skin, etc., it is possible to obtain an antioxidative effect, in particular, an excellent antioxidative effect such as an effect of improving the storage stability of the product, and various disease preventive effects and antioxidant effects. Therefore, it is expected to greatly contribute to the industry.
[0330] 本発明の第 2の発明により、力力ロールを含む安全性に優れた細胞賦活作用、抗 老化作用が期待でき、細胞賦活および抗老化成分を有効成分とした化粧品 ·医薬部 外品（皮膚外用剤、浴用剤、育毛剤等）、医薬品、飲食品を提供することできることか ら、産業界に大きく寄与することが期待される。  [0330] According to the second invention of the present invention, it is expected to have a cell activation action and an anti-aging action excellent in safety including a power roll, and a cosmetic / quasi-drug containing the cell activation and anti-aging ingredient as an active ingredient. It is expected to make a significant contribution to the industry because it can provide (external skin preparations, bath preparations, hair restorers, etc.), pharmaceuticals, and food and drinks.
[0331] 本発明の第 3の発明は、力力ロールを含む植物抽出物からクロロフィルを効率よく除 去する方法を提供するものであり、化粧品産業、医薬品産業、食品産業などに大きく 寄与すること力 Sでさる。  [0331] The third invention of the present invention provides a method for efficiently removing chlorophyll from a plant extract containing a power roll, and greatly contributes to the cosmetics industry, pharmaceutical industry, food industry and the like. Touch with force S.
[0332] 本発明の第 4の発明に係るコラーゲン産生促進剤は，コラーゲン産生を促進する効 果に優れ、かつ、安全な力力ロールを有効成分としている。したがって、長期にわたる 使用に十分に耐え得るという効果を奏し、化粧品産業、医薬品産業、食品産業など に大きく寄与することカできる。  [0332] The collagen production promoter according to the fourth invention of the present invention is excellent in the effect of promoting collagen production and contains a safe power roll as an active ingredient. Therefore, it has the effect of sufficiently withstanding long-term use, and can greatly contribute to the cosmetics industry, pharmaceutical industry, food industry, and the like.

Claims

請求の範囲  The scope of the claims
[I] 力力ロールを含有することを特徴とする活性酸素消去剤。  [I] An active oxygen scavenger containing a power roll.
[2] 力力ロールを有効成分として含有し、 [2] containing a power roll as an active ingredient,
スーパーォキシド、過酸化水素、および一重項酸素からなる群から選択される少なく とも 1種以上の活性酸素を消去することを特徴とする請求項 1に記載の活性酸素消去 剤。  2. The active oxygen scavenger according to claim 1, wherein at least one active oxygen selected from the group consisting of superoxide, hydrogen peroxide, and singlet oxygen is scavenged.
[3] 請求項 1または 2に記載の力カロールカ S、 Cacalia属植物から抽出された力力ロールで あることを特徴とする活性酸素消去剤。  [3] A reactive oxygen scavenger, which is a powerful roll extracted from the plant of the genus Caloruka S or Cacalia according to claim 1 or 2.
[4] 請求項 3に記載の Cacalia属植物力 モミジガサであることを特徴とする活性酸素消去 剤。 [4] An active oxygen scavenger, which is the Cacalia genus plant power of claim 3.
[5] 請求項 1または 2に記載の力カロールカ 、化学合成により製造された力力ロールであ ることを特徴とする活性酸素消去剤。  [5] The active oxygen scavenger according to claim 1 or 2, wherein the active oxygen scavenger is a power roll manufactured by chemical synthesis.
[6] 請求項 1〜5のいずれかに記載の活性酸素消去剤を用いて、  [6] Using the active oxygen scavenger according to any one of claims 1 to 5,
スーパーォキシド、過酸化水素、および一重項酸素からなる群から選択される少なく とも 1種以上の活性酸素を消去することを特徴とする活性酸素消去方法。  A reactive oxygen scavenging method comprising scavenging at least one active oxygen selected from the group consisting of superoxide, hydrogen peroxide, and singlet oxygen.
[7] 力力ロールを含有することを特徴とする賦活化剤。  [7] An activator comprising a power roll.
[8] 請求項 7に記載の力カロールカ S、 Cacalia属植物から抽出された力力ロールであること を特徴とする賦活化剤。  [8] The activator characterized in that it is a force roll extracted from the plant of the genus Calorca S or Cacalia according to claim 7.
[9] 請求項 8に記載の Cacalia属植物力 モミジガサであることを特徴とする賦活化剤。 [9] The activator according to claim 8, wherein the activator is Cacalia spp.
[10] 請求項 7に記載の力カロールカ S、化学合成により製造された力力ロールであることを 特徴とする賦活化剤。 [10] The activator according to claim 7, wherein the activator is a force roll produced by chemical synthesis.
[I I] Cacalia属植物の抽出物を有効成分として含有することを特徴とする賦活化剤。  [I I] An activator comprising an extract of a genus Cacalia as an active ingredient.
[12] 上記 Cacalia属植物の抽出物が力力ロールを含むことを特徴とする請求項 11に記載 の賦活化剤。  [12] The activator according to claim 11, wherein the extract of the genus Cacalia contains a force roll.
[13] 上記 Cacalia属植物がモミジガサであることを特徴とする請求項 11または請求項 12 に記載の賦活化剤。  [13] The activator according to claim 11 or 12, wherein the plant belonging to the genus Cacalia is Maplesa.
[14] 力力ロールを含有することを特徴とするコラーゲン産生促進剤。 [14] A collagen production promoter comprising a force roll.
[15] 請求項 14に記載の力カロールカ S、 Cacalia属植物から抽出された力力ロールであるこ とを特徴とするコラーゲン産生促進剤。 [15] The force Kalorka S according to claim 14, which is a force roll extracted from a plant belonging to the genus Cacalia. Collagen production promoter characterized by these.
[16] 請求項 15に記載の Cacalia属植物力 モミジガサであることを特徴とするコラーゲン産 生促進剤。 [16] A collagen production promoter according to claim 15, which is the genus Cacalia plant power Momijigasa.
[17] 請求項 14に記載の力カロールカ S、化学合成により製造された力力ロールであることを 特徴とするコラーゲン産生促進剤。  [17] The collagen production promoter according to claim 14, which is a force roll produced by chemical synthesis.
[18] 請求項 1に記載の活性酸素消去剤、請求項 7に記載の賦活化剤、請求項 11に記載 の賦活化剤、および請求項 14に記載のコラーゲン産生促進剤からなる群から選択さ れる少なくとも 1種以上を有効成分として含有することを特徴とする抗老化剤。  [18] Selected from the group consisting of the active oxygen scavenger according to claim 1, the activator according to claim 7, the activator according to claim 11, and the collagen production promoter according to claim 14. An anti-aging agent comprising at least one selected from the above as an active ingredient.
[19] 請求項 1〜5のいずれかに記載の活性酸素消去剤、請求項 7〜； 13のいずれかに記 載の賦活化剤、請求項 14〜； 17のいずれかに記載のコラーゲン産生促進剤、または 請求項 18に記載の抗老化剤を有効成分として含有する外用剤。  [19] The active oxygen scavenger according to any one of claims 1 to 5, the activator according to any one of claims 7 to 13 and the collagen production according to any one of claims 14 to 17 An external preparation containing the accelerator or the anti-aging agent according to claim 18 as an active ingredient.
[20] 請求項 1〜5のいずれかに記載の活性酸素消去剤、請求項 7〜； 13のいずれかに記 載の賦活化剤、請求項 14〜； 17のいずれかに記載のコラーゲン産生促進剤、または 請求項 18に記載の抗老化剤を有効成分として含有する化粧品類。  [20] The active oxygen scavenger according to any one of claims 1 to 5, the activator according to any one of claims 7 to 13 and the collagen production according to any one of claims 14 to 17 A cosmetic comprising the accelerator or the anti-aging agent according to claim 18 as an active ingredient.
[21] 請求項 1〜5のいずれかに記載の活性酸素消去剤、請求項 7〜； 13のいずれかに記 載の賦活化剤、請求項 14〜； 17のいずれかに記載のコラーゲン産生促進剤、または 請求項 18に記載の抗老化剤を有効成分として含有する医薬部外品類。  [21] The active oxygen scavenger according to any one of claims 1 to 5, the activator according to any one of claims 7 to 13 and the collagen production according to any one of claims 14 to 17 A quasi-drug containing the accelerator or the anti-aging agent according to claim 18 as an active ingredient.
[22] 請求項 1〜5のいずれかに記載の活性酸素消去剤、請求項 7〜； 13のいずれかに記 載の賦活化剤、請求項 14〜； 17のいずれかに記載のコラーゲン産生促進剤、または 請求項 18に記載の抗老化剤を有効成分として含有する飲食品類。  [22] The active oxygen scavenger according to any one of claims 1 to 5, the activator according to any one of claims 7 to 13 and the collagen production according to any one of claims 14 to 17 Food / beverage products containing the accelerator or the anti-aging agent according to claim 18 as an active ingredient.
[23] 請求項 1〜5のいずれかに記載の活性酸素消去剤、請求項 7〜； 13のいずれかに記 載の賦活化剤、請求項 14〜； 17のいずれかに記載のコラーゲン産生促進剤、または 請求項 18に記載の抗老化剤を有効成分として含有する医薬品類。  [23] The active oxygen scavenger according to any one of claims 1 to 5, the activator according to any one of claims 7 to 13 and the collagen production according to any one of claims 14 to 17 A pharmaceutical comprising the accelerator or the anti-aging agent according to claim 18 as an active ingredient.
[24] 力力ロールを含む植物抽出物から水および親水性有機溶媒の混合物を用いて二 次抽出物を得る工程を包含し、  [24] including a step of obtaining a secondary extract from a plant extract containing a power roll using a mixture of water and a hydrophilic organic solvent,
上記二次抽出物は上記力力ロールを含む植物抽出物と比較してクロロフィル含量 が減少していることを特徴とする力力ロールを含む植物抽出物の製造方法。  The method for producing a plant extract containing a power roll, wherein the secondary extract has a reduced chlorophyll content as compared with the plant extract containing the power roll.
[25] 上記二次抽出物中のクロロフィル含量が目的の数値以下になるまで上記工程を繰 り返すことを特徴とする請求項 24に記載の力力ロールを含む植物抽出物の製造方法 [25] Repeat the above steps until the chlorophyll content in the secondary extract is below the target value. The method for producing a plant extract containing a power roll according to claim 24,
[26] さらに、力力ロールを含む植物抽出物から、水、または、水および親水性有機溶媒 の混合物を用いて力力ロールより高親水性の夾雑物を除去する工程を包含すること を特徴とする請求項 24または 25に記載の力力ロールを含む植物抽出物の製造方法 [26] The method further comprises the step of removing contaminants having higher hydrophilicity than the power roll from the plant extract containing the power roll using water or a mixture of water and a hydrophilic organic solvent. A method for producing a plant extract containing the power roll according to claim 24 or 25.
[27] 上記親水性有機溶媒が、エタノール、アセトン、イソプロピルアルコール、プロピレン グリコール、ブチレングリコール、およびブタノールからなる群より選ばれる 1種または 2種以上の混合物であることを特徴とする請求項 24 26のいずれか 1項に記載の力 力ロールを含む植物抽出物の製造方法。 [27] The hydrophilic organic solvent is one or a mixture of two or more selected from the group consisting of ethanol, acetone, isopropyl alcohol, propylene glycol, butylene glycol, and butanol. The manufacturing method of the plant extract containing the power roll of any one of these.
[28] 上記二次抽出物を得る工程にお!/、て用いられる水および親水性有機溶媒の混合 物力 S、エタノール含量 45%以上の含水エタノールであることを特徴とする請求項 24 に記載の力力ロールを含む植物抽出物の製造方法。  [28] The mixture of water and hydrophilic organic solvent used in the step of obtaining the secondary extract! /, And hydrous ethanol having an ethanol content of 45% or more. The manufacturing method of the plant extract containing a power roll.
[29] 上記夾雑物を除去する工程にお!/、て用いられる水および親水性有機溶媒の混合 物力 S、エタノール含量 40%以下の含水エタノールであることを特徴とする請求項 26 に記載の力力ロールを含む植物抽出物の製造方法。 [29] The mixture of water and a hydrophilic organic solvent used in the step of removing impurities! /, And hydrous ethanol having an ethanol content of 40% or less. A method for producing a plant extract containing a power roll.
[30] 上記植物がキク科植物であることを特徴とする請求項 24 29のいずれか 1項に記 載の力力ロールを含む植物抽出物の製造方法。 [30] The method for producing a plant extract containing the power roll according to any one of [24], wherein the plant is an Asteraceae plant.
[31] 上記キク科植物が Cacalia属植物であることを特徴とする請求項 30に記載の力カロ ールを含む植物抽出物の製造方法。 31. The method for producing a plant extract containing a force carol according to claim 30, wherein the Asteraceae plant is a Cacalia genus plant.
[32] 上記 Cacalia属植物がモミジガサであることを特徴とする請求項 31に記載の力カロ ールを含む植物抽出物の製造方法。 [32] The method for producing a plant extract containing hard carol according to [31], wherein the Cacalia genus plant is Momijigasa.
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JP2008308436A (en) * 2007-06-14 2008-12-25 Maruzen Pharmaceut Co Ltd Antioxidant, anti-aging agent, anti-inflammatory agent, skin cosmetic, and food/drink for beauty culture
JP2010059065A (en) * 2008-09-02 2010-03-18 Asuka Yume:Kk Slimming cosmetic
JP2010195726A (en) * 2009-02-26 2010-09-09 Noevir Co Ltd Dermatopontin production promoter
JP2011213699A (en) * 2010-04-02 2011-10-27 Kinki Univ Skin care external preparation having collagen production promotion action obtained from daisy inflorescence
WO2013118887A1 (en) * 2012-02-09 2013-08-15 国立大学法人秋田大学 Whitening composition having melanin-inhibiting and mitf-inhibiting effects and anticancer agent
JP2013163645A (en) * 2012-02-09 2013-08-22 Akita Univ Composition for skin-whitening and anti-cancer agent having melanin inhibition and mitf inhibitory action
JP2014198683A (en) * 2013-03-29 2014-10-23 国立大学法人秋田大学 Microphthalmia-associated transcription factor inhibiting agent, melanogenesis inhibiting agent, cosmetic composition and anticancer agent
CN103431264A (en) * 2013-07-18 2013-12-11 浙江劲膳美生物科技有限公司 Special diet edible for people with specific endowment constitution
JP2015196655A (en) * 2014-03-31 2015-11-09 小林製薬株式会社 Composition containing vitamin b6
CN104792909A (en) * 2015-04-23 2015-07-22 合肥伟海讴斯医药科技有限公司 Method for distinguishing genuine medicinal material Huangshan florists chrysanthemum from counterfeit callistephus chinensis

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