WO2008041244B1 - A method for the identification of potential biomarkers and drug targets in brain plasticity related disorders - Google Patents

A method for the identification of potential biomarkers and drug targets in brain plasticity related disorders

Info

Publication number
WO2008041244B1
WO2008041244B1 PCT/IN2007/000452 IN2007000452W WO2008041244B1 WO 2008041244 B1 WO2008041244 B1 WO 2008041244B1 IN 2007000452 W IN2007000452 W IN 2007000452W WO 2008041244 B1 WO2008041244 B1 WO 2008041244B1
Authority
WO
WIPO (PCT)
Prior art keywords
genes
epileptogenesis
fly
cgl
products
Prior art date
Application number
PCT/IN2007/000452
Other languages
French (fr)
Other versions
WO2008041244A3 (en
WO2008041244A2 (en
Inventor
Abhay Sharma
Priyanka Singh
Farhan, Md
Original Assignee
Council Scient Ind Res
Abhay Sharma
Priyanka Singh
Farhan, Md
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Council Scient Ind Res, Abhay Sharma, Priyanka Singh, Farhan, Md filed Critical Council Scient Ind Res
Priority to EP07827592A priority Critical patent/EP2076608A2/en
Publication of WO2008041244A2 publication Critical patent/WO2008041244A2/en
Publication of WO2008041244A3 publication Critical patent/WO2008041244A3/en
Publication of WO2008041244B1 publication Critical patent/WO2008041244B1/en
Priority to NO20091627A priority patent/NO20091627L/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/136—Screening for pharmacological compounds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The invention relates to identification of potential biomarkers and drug targets in brain plasticity related disorders. In particular, the invention describes a system level model of brain plasticity related disorders in the fruit fly Drosophila melanogaster, thereby identifying potential biomarkers and drug targets in epileptogenesis and associated conditions. The invention also provides a means of screening therapeutic agents against epilepsy and related disorders using gene expression profiling in Drosophila.

Claims

AMENDED CLAIMS received by the International Bureau on 11 August 2008 (11.08.08)
1. A method for identification of potential biomarkers for epileptogenesis and related brain plasticity disorders, said method consisting the steps of:
[a] treating Drosophila flies with either convulsant or antiepileptic drugs in a chronic manner;
[b] extracting RNA from the heads of treated flies of step [a] at several time-points secondary to drug treatments*
[c] generating microarray gene expression profiles specific for convulsant and antiepileptic drugs respectively using the RNA extracted from step [b];
[d] identifying genes that are up- and down-regulated by convulsant and antiepileptic drugs from the microarray of step [c];
[e] identifying genes which are down- and up-regulated respectively by convulsant and antiepileptic drugs in the fly model and the homologs down regulated in established rodent models of epilepsy development as candidate biomarkers of epileptogenesis.
2. A method for identification of potential drug targets against epileptogenesis and related brain plasticity disorders, said method consisting the steps of:
(a) comparing fly genes that are regulated by different antiepileptic drugs in Drosophila head after categorizing them on the basis of biological processes they represent;
(b) identifying fly genes that are similarly regulated by different antiepileptic drugs as potential drug targets;
(c) validating the candidature of the potential drug targets by confirming the biological processes they represent.
3. A method according to claim 1, wherein products of Drosophila genes Aldolase (CG6058) and Glutathione S transferase D4 (CGl 1512) are identified as biomarkers of kindling- epileptogenesis like plasticity in the fly model.
4. A method according to claim 1, wherein the products of homologs of fly genes CG6058 and CGl 1512 are identified as potential biomarkers of epileptogenesis and associated disorders.
43
5. A method according to claim 2, wherein products of Drosophila genes Glutamate oxaloacetate transaminase 2 (CG4233) and Sorbitol dehydrogenase 1 (CGl 982) are identified as targets of antiepileptic drugs in fly model of kindling-epileptogenesis like plasticity.
6. A method according to claim 2, wherein products of homologs of fly genes CG4233 and CGl 982 are identified as potential targets for developing drugs against epilepsy and related disorders.
7. Use of products of human homologs of fly genes CG6058 and CGl 1512 as biomarkers of epileptogenesis in at-risk individuals.
8. Use of products of human homologs of fly genes CG4233 and CGl 982 as drug targets for developing therapeutic agents against epilepsy and related brain disorders.
44
PCT/IN2007/000452 2006-10-05 2007-10-01 A method for the identification of potential biomarkers and drug targets in brain plasticity related disorders WO2008041244A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP07827592A EP2076608A2 (en) 2006-10-05 2007-10-01 A method for the identification of potential biomarkers and drug targets in brain plasticity related disorders
NO20091627A NO20091627L (en) 2006-10-05 2009-04-23 Procedure for Identifying Potential Biomarkers and Drug Templates in Brain Plasticity-Related Disorders

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IN2193DE2006 2006-10-05
IN2193/DEL/2006 2006-10-05

Publications (3)

Publication Number Publication Date
WO2008041244A2 WO2008041244A2 (en) 2008-04-10
WO2008041244A3 WO2008041244A3 (en) 2008-07-31
WO2008041244B1 true WO2008041244B1 (en) 2008-10-09

Family

ID=39198212

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IN2007/000452 WO2008041244A2 (en) 2006-10-05 2007-10-01 A method for the identification of potential biomarkers and drug targets in brain plasticity related disorders

Country Status (3)

Country Link
EP (1) EP2076608A2 (en)
NO (1) NO20091627L (en)
WO (1) WO2008041244A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108977549B (en) * 2018-07-04 2021-01-15 中国农业大学 Primer pair for detecting expression quantity of CG5210 gene of fruit fly

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6291739B1 (en) * 2000-03-24 2001-09-18 Council Of Scientific And Industrial Research Method for screening of potential anti-epileptic drugs using a Drosophila melanogaster model

Also Published As

Publication number Publication date
EP2076608A2 (en) 2009-07-08
WO2008041244A3 (en) 2008-07-31
WO2008041244A2 (en) 2008-04-10
NO20091627L (en) 2009-07-02

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